RchiOBHm_Chr6g0259521

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000590
Physical Location & Seq
Reverse (-)
14747055 .. 14748699
1645 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23271

Sequence Viewer

Length: 1056 bp
ATGGGAGAGGTTGATCCAGCTTTCATCCAAGATCCTGAGCACAGGCCGAAACTCTCCATCATCAAAGCTGAAGGCATACCATCGATTGACCTCTCTCCAGTAACCTTGCAAGATTTCATTTCCTACCCCAAATCCATTGAAGGTGTTGTCAGAGAAATAGGGAATGCATGCAAGGAGTGGGGGTTCTTCCAAGTGATCAACCATGGGGTGCCATTGGAGAAGCTCCATAGAATTCAGGCTGCTGCTAGGAAGTTCTTTGCACAGCCTCTGGAGGATAAGAGGAAGATCAGGAGAGATGAAAGAAACGCATTGGGGTACTTTGACACCGAGAGCACCAAGAATATCCGAGACTGGAAGGAGGTGTTTGATTTCTCAATGGAAGACACTACTTTAGTCCCAGCTTCACCTGATCCCGAGGACAAGGAAGAGGCAACATGGACTAACCAATGGCCTGAGTACCCTCCAGAATTAAGGGAAGCATGTGAAGAATATACTCAGGAAGTAGAGAAGCTTGCTCTAAAGTTATTAGGACTTATAGCCAGGAGCCTAGGCTTGCCAGAAAACAGGTTCAACAGCTACTTCAAAGACCAAAGCACTCGTATCAGACTCAATCACTATCCCCCTTGCCCTTCCCCTCAGTTAGCTCTTGGTGTAGGTCGCCACAAGGATGGTGGTGCTTTAACCGTGCTAGCTCAAGATGAAATTGGTGGATTGGAAGTGAAGAGAAAGACAGATGGAGAGTGGATTCGGGTTCAACCTACACCAAATGCCTATATCATCAATGTTGGTGATATTATGCAGGTTTGGAGCAATGATAGATATGAGAGTGTTGAGCACAGGGTGATGGTGGACTCAGAGAAGGAAAGGTTTTCCATTCCTTTCTTCTTCAATCCAGCACACTACACACTAGTGAAGCCTGTGGAGGAGCTGATCGATGAACAAAACCCTGCCAAGTACAGGCCTTACAGCTGGGGAAAGTTTATGACTCACAGAAGACTCAGTAATTTCAAGAAGCTCGGTGTTGACAACATCCAAATATATGATTTTAGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

351

Amino Acids

40.67

Weight (kDa)

5.64

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 26 - 152 6.6e-31 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 201 - 298 7.4e-34 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19000 AT3G19000
fragaria_vesca FvH4_2g07071 FvH4_2g07080 FvH4_2g07080 FvH4_2g07081 FvH4_2g07082 FvH4_2g07082
malus_domestica MD05G1074000.v1.1 MD10G1088100.v1.1 MD10G1088200.v1.1 MD14G1207600.v1.1
prunus_persica Prupe.8G114100_v2.0.a1 Prupe.8G114200_v2.0.a1 Prupe.8G114300_v2.0.a1 Prupe.8G114500_v2.0.a1 Prupe.8G114800_v2.0.a1 Prupe.8G115100_v2.0.a1
pyrus_communis pycom05g06640 pycom05g06660 pycom05g06670 pycom10g07050
rosa_chinensis RchiOBHm_Chr6g0244141 RchiOBHm_Chr6g0259511 RchiOBHm_Chr6g0259521 RchiOBHm_Chr6g0259531 RchiOBHm_Chr6g0259551 RchiOBHm_Chr6g0259561
rosa_laevigata RLG00000014522 RLG00000014523 RLG00000014524 RLG00000014525 RLG00000014526 RLG00000014527
rosa_multiflora Rmu_ssc0000050.1_g000019 Rmu_ssc0000050.1_g000020 Rmu_ssc0000050.1_g000026 Rmu_ssc0000050.1_g000027 Rmu_ssc0000050.1_g000028
rosa_roxburghii Rroxscaffold_7G00206780 Rroxscaffold_7G00206790 Rroxscaffold_7G00206850 Rroxscaffold_7G00206860 Rroxscaffold_7G00206870 Rroxscaffold_7G00206880
rosa_rugosa Rorug05G0589100 Rorug05G0589200.1 Rorug05G0589300 Rorug05G0589400 Rorug05G0589500 Rorug05G0589600
rosa_samantha Rh4CG096000 Rh5BG231700 Rh6AG104900 Rh6AG105000 Rh6AG105100 Rh6AG105500 Rh6AG105600 Rh6BG098500 Rh6BG098700 Rh6BG098800 Rh6BG099000 Rh6BG099100 Rh6CG094200 Rh6CG094300 Rh6CG094400 Rh6CG094500 Rh6CG094600 Rh6DG088300 Rh6DG088400 Rh6DG088500 Rh6DG088600 Rh6DG088700
rosa_wichuraiana Rw0G014270 Rw0G014280 Rw0G014290 Rw6G009080 Rw6G009090 Rw6G009100 Rw6G009110 Rw6G009120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 790
AccB1I GGYRCC 1 cut(s) 208
AclWI GGATC 3 cut(s) 8, 26, 404
AcsI RAATTY 1 cut(s) 231
AcuI CTGAAG 1 cut(s) 90
AdeI CACNNNGTG 1 cut(s) 841
AfaI GTAC 3 cut(s) 317, 458, 956
AfiI CCNNNNNNNGG 1 cut(s) 957
AgsI TTSAA 6 cut(s) 140, 571, 583, 755, 889, 1009
AhlI ACTAGT 1 cut(s) 907
AjnI CCWGG 1 cut(s) 539
AleI CACNNNNGTG 1 cut(s) 908
AloI GAACNNNNNNTCC 2 cut(s) 167, 199
Alw21I GWGCWC 3 cut(s) 42, 335, 837
Alw26I GTCTC 1 cut(s) 342
AlwI GGATC 3 cut(s) 8, 26, 404
AlwNI CAGNNNCTG 1 cut(s) 268
Ama87I CYCGRG 1 cut(s) 413
AoxI GGCC 3 cut(s) 44, 449, 959
ApeKI GCWGC 2 cut(s) 239, 242
ApoI RAATTY 1 cut(s) 231
AspA2I CCTAGG 1 cut(s) 547
AsuHPI GGTGA 3 cut(s) 396, 800, 853
AsuNHI GCTAGC 1 cut(s) 688
AvaI CYCGRG 1 cut(s) 413
AvrII CCTAGG 1 cut(s) 547
BanI GGYRCC 1 cut(s) 208
BbsI GAAGAC 2 cut(s) 387, 1000
Bbv12I GWGCWC 3 cut(s) 42, 335, 837
BbvI GCAGC 2 cut(s) 226, 229
BccI CCATC 5 cut(s) 65, 88, 662, 728, 838
BciT130I CCWGG 1 cut(s) 541
BclI TGATCA 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 342
BcuI ACTAGT 1 cut(s) 907
BfaI CTAG 4 cut(s) 246, 548, 689, 908
BfuAI ACCTGC 1 cut(s) 790
BisI GCNGC 2 cut(s) 240, 243
BlnI CCTAGG 1 cut(s) 547
BlsI GCNGC 2 cut(s) 241, 244
Bme1390I CCNGG 1 cut(s) 541
BmeT110I CYCGRG 1 cut(s) 413
BmiI GGNNCC 2 cut(s) 210, 545
BmrFI CCNGG 1 cut(s) 541
BmtI GCTAGC 1 cut(s) 692
BpiI GAAGAC 2 cut(s) 387, 1000
BpmI CTGGAG 3 cut(s) 81, 290, 447
Bpu10I CCTNAGC 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 678
Bsa29I ATCGAT 2 cut(s) 83, 933
BsaJI CCNNGG 3 cut(s) 202, 414, 547
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 1 cut(s) 957
Bse1I ACTGG 2 cut(s) 98, 356
Bse3DI GCAATG 1 cut(s) 817
BseBI CCWGG 1 cut(s) 541
BseCI ATCGAT 2 cut(s) 83, 933
BseDI CCNNGG 3 cut(s) 202, 414, 547
BseGI GGATG 3 cut(s) 24, 673, 1029
BseLI CCNNNNNNNGG 1 cut(s) 957
BseMI GCAATG 1 cut(s) 817
BseMII CTCAG 6 cut(s) 27, 444, 509, 650, 867, 1012
BseNI ACTGG 2 cut(s) 98, 356
BseRI GAGGAG 1 cut(s) 938
BseXI GCAGC 2 cut(s) 226, 229
BseYI CCCAGC 2 cut(s) 397, 969
BshFI GGCC 3 cut(s) 46, 451, 961
BshNI GGYRCC 1 cut(s) 208
BshVI ATCGAT 2 cut(s) 83, 933
BsiHKAI GWGCWC 3 cut(s) 42, 335, 837
BsiHKCI CYCGRG 1 cut(s) 413
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 1 cut(s) 957
BsmAI GTCTC 1 cut(s) 342
BsmFI GGGAC 1 cut(s) 380
BsmI GAATGC 1 cut(s) 169
BsnI GGCC 3 cut(s) 46, 451, 961
BsoBI CYCGRG 1 cut(s) 413
Bsp1286I GDGCHC 3 cut(s) 42, 335, 837
Bsp143I GATC 6 cut(s) 13, 31, 195, 285, 409, 930
Bsp19I CCATGG 1 cut(s) 202
BspANI GGCC 3 cut(s) 46, 451, 961
BspCNI CTCAG 6 cut(s) 28, 445, 508, 649, 866, 1011
BspDI ATCGAT 2 cut(s) 83, 933
BspLI GGNNCC 2 cut(s) 210, 545
BspMI ACCTGC 1 cut(s) 790
BspOI GCTAGC 1 cut(s) 692
BspPI GGATC 3 cut(s) 8, 26, 404
BspT107I GGYRCC 1 cut(s) 208
BsrDI GCAATG 1 cut(s) 817
BsrI ACTGG 2 cut(s) 98, 356
BssECI CCNNGG 3 cut(s) 202, 414, 547
BssMI GATC 6 cut(s) 13, 31, 195, 285, 409, 930
BssT1I CCWWGG 2 cut(s) 202, 547
Bst2UI CCWGG 1 cut(s) 541
Bst4CI ACNGT 1 cut(s) 685
Bst6I CTCTTC 2 cut(s) 420, 716
BstC8I GCNNGC 4 cut(s) 169, 513, 554, 690
BstDEI CTNAG 6 cut(s) 36, 453, 495, 636, 853, 998
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 3 cut(s) 24, 673, 1029
BstKTI GATC 6 cut(s) 16, 34, 198, 288, 412, 933
BstMAI GTCTC 1 cut(s) 342
BstMBI GATC 6 cut(s) 13, 31, 195, 285, 409, 930
BstNI CCWGG 1 cut(s) 541
BstNSI RCATGY 2 cut(s) 171, 483
BstSCI CCNGG 1 cut(s) 539
BstV1I GCAGC 2 cut(s) 226, 229
BstV2I GAAGAC 2 cut(s) 387, 1000
BstX2I RGATCY 1 cut(s) 31
BstXI CCANNNNNNTGG 1 cut(s) 668
BstYI RGATCY 1 cut(s) 31
Bsu15I ATCGAT 2 cut(s) 83, 933
BsuRI GGCC 3 cut(s) 46, 451, 961
BsuTUI ATCGAT 2 cut(s) 83, 933
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 3 cut(s) 24, 673, 1029
BveI ACCTGC 1 cut(s) 790
Cac8I GCNNGC 4 cut(s) 169, 513, 554, 690
CaiI CAGNNNCTG 1 cut(s) 268
ClaI ATCGAT 2 cut(s) 83, 933
Csp6I GTAC 3 cut(s) 316, 457, 955
CspCI CAANNNNNGTGG 2 cut(s) 652, 687
CviAII CATG 4 cut(s) 168, 203, 435, 480
CviQI GTAC 3 cut(s) 316, 457, 955
DdeI CTNAG 6 cut(s) 36, 453, 495, 636, 853, 998
DpnI GATC 6 cut(s) 15, 33, 197, 287, 411, 932
DpnII GATC 6 cut(s) 13, 31, 195, 285, 409, 930
DraIII CACNNNGTG 1 cut(s) 841
Eam1104I CTCTTC 2 cut(s) 420, 716
EarI CTCTTC 2 cut(s) 420, 716
Eco130I CCWWGG 2 cut(s) 202, 547
Eco147I AGGCCT 1 cut(s) 961
Eco57I CTGAAG 1 cut(s) 90
Eco88I CYCGRG 1 cut(s) 413
EcoRI GAATTC 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 539
EcoT14I CCWWGG 2 cut(s) 202, 547
EcoT22I ATGCAT 1 cut(s) 169
ErhI CCWWGG 2 cut(s) 202, 547
FaeI CATG 4 cut(s) 171, 206, 438, 483
FaqI GGGAC 1 cut(s) 380
FatI CATG 4 cut(s) 167, 202, 434, 479
FbaI TGATCA 1 cut(s) 195
Fnu4HI GCNGC 2 cut(s) 240, 243
FokI GGATG 3 cut(s) 11, 680, 1016
Fsp4HI GCNGC 2 cut(s) 240, 243
FspBI CTAG 4 cut(s) 246, 548, 689, 908
GluI GCNGC 2 cut(s) 240, 243
GsaI CCCAGC 2 cut(s) 401, 973
GsuI CTGGAG 3 cut(s) 81, 290, 447
HaeIII GGCC 3 cut(s) 46, 451, 961
Hin1II CATG 4 cut(s) 171, 206, 438, 483
HincII GTYRAC 1 cut(s) 1024
HindII GTYRAC 1 cut(s) 1024
HindIII AAGCTT 1 cut(s) 509
HinfI GANTC 5 cut(s) 606, 745, 851, 985, 996
HphI GGTGA 3 cut(s) 396, 800, 853
Hpy166II GTNNAC 2 cut(s) 850, 1024
Hpy188I TCNGA 4 cut(s) 152, 347, 605, 856
Hpy188III TCNNGA 8 cut(s) 35, 269, 289, 413, 464, 497, 695, 1009
Hpy8I GTNNAC 2 cut(s) 850, 1024
HpyAV CCTTC 5 cut(s) 65, 134, 349, 639, 853
HpyCH4III ACNGT 1 cut(s) 685
HpyCH4V TGCA 5 cut(s) 109, 167, 171, 260, 799
HpyF3I CTNAG 6 cut(s) 36, 453, 495, 636, 853, 998
Hsp92II CATG 4 cut(s) 171, 206, 438, 483
Ksp22I TGATCA 1 cut(s) 195
Kzo9I GATC 6 cut(s) 13, 31, 195, 285, 409, 930
LmnI GCTCC 4 cut(s) 228, 543, 807, 925
Lsp1109I GCAGC 2 cut(s) 226, 229
MaeI CTAG 4 cut(s) 246, 548, 689, 908
MaeIII GTNAC 1 cut(s) 100
MalI GATC 6 cut(s) 15, 33, 197, 287, 411, 932
MboI GATC 6 cut(s) 13, 31, 195, 285, 409, 930
MboII GAAGA 9 cut(s) 178, 295, 392, 437, 497, 733, 874, 877, 1005
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 3 cut(s) 42, 335, 837
MluCI AATT 4 cut(s) 231, 467, 702, 1003
MlyI GAGTC 4 cut(s) 600, 845, 979, 990
Mph1103I ATGCAT 1 cut(s) 169
MseI TTAA 2 cut(s) 470, 680
MslI CAYNNNNRTG 2 cut(s) 666, 908
MspA1I CMGCKG 1 cut(s) 969
MspR9I CCNGG 1 cut(s) 541
Mva1269I GAATGC 1 cut(s) 169
MvaI CCWGG 1 cut(s) 541
NcoI CCATGG 1 cut(s) 202
NdeII GATC 6 cut(s) 13, 31, 195, 285, 409, 930
NheI GCTAGC 1 cut(s) 688
NlaIII CATG 4 cut(s) 171, 206, 438, 483
NlaIV GGNNCC 2 cut(s) 210, 545
NsiI ATGCAT 1 cut(s) 169
NspI RCATGY 2 cut(s) 171, 483
OliI CACNNNNGTG 1 cut(s) 908
PaeI GCATGC 1 cut(s) 171
PceI AGGCCT 1 cut(s) 961
PctI GAATGC 1 cut(s) 169
PfeI GAWTC 1 cut(s) 745
PkrI GCNGC 2 cut(s) 241, 244
PleI GAGTC 4 cut(s) 600, 845, 979, 990
PpsI GAGTC 4 cut(s) 600, 845, 979, 990
Psp6I CCWGG 1 cut(s) 539
PspFI CCCAGC 2 cut(s) 397, 969
PspGI CCWGG 1 cut(s) 539
PspN4I GGNNCC 2 cut(s) 210, 545
PstNI CAGNNNCTG 1 cut(s) 268
PsuI RGATCY 1 cut(s) 31
PvuII CAGCTG 1 cut(s) 969
RsaI GTAC 3 cut(s) 317, 458, 956
RsaNI GTAC 3 cut(s) 316, 457, 955
RseI CAYNNNNRTG 2 cut(s) 666, 908
SaqAI TTAA 2 cut(s) 470, 680
SatI GCNGC 2 cut(s) 240, 243
Sau3AI GATC 6 cut(s) 13, 31, 195, 285, 409, 930
SchI GAGTC 4 cut(s) 600, 845, 979, 990
ScrFI CCNGG 1 cut(s) 541
SduI GDGCHC 3 cut(s) 42, 335, 837
SmiMI CAYNNNNRTG 2 cut(s) 666, 908
SmlI CTYRAG 1 cut(s) 693
SmoI CTYRAG 1 cut(s) 693
SpeI ACTAGT 1 cut(s) 907
SphI GCATGC 1 cut(s) 171
Sse9I AATT 4 cut(s) 231, 467, 702, 1003
SseBI AGGCCT 1 cut(s) 961
SspMI CTAG 4 cut(s) 246, 548, 689, 908
StuI AGGCCT 1 cut(s) 961
StyD4I CCNGG 1 cut(s) 539
StyI CCWWGG 2 cut(s) 202, 547
TaaI ACNGT 1 cut(s) 685
TaqI TCGA 2 cut(s) 83, 933
TasI AATT 4 cut(s) 231, 467, 702, 1003
TatI WGTACW 1 cut(s) 954
TfiI GAWTC 1 cut(s) 745
Tru1I TTAA 2 cut(s) 470, 680
Tru9I TTAA 2 cut(s) 470, 680
TseI GCWGC 2 cut(s) 239, 242
TspDTI ATGAA 5 cut(s) 13, 106, 312, 714, 951
XapI RAATTY 1 cut(s) 231
XceI RCATGY 2 cut(s) 171, 483
XcmI CCANNNNNNNNNTGG 1 cut(s) 668
XmaJI CCTAGG 1 cut(s) 547
XspI CTAG 4 cut(s) 246, 548, 689, 908
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.