RchiOBHm_Chr6g0259531

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000590
Physical Location & Seq
Forward (+)
14748701 .. 14751242
2542 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23272

Sequence Viewer

Length: 1071 bp
ATGGGAGAAGTTGATCCAGCTTTCATCCAAGACCCTGAACACAGACCTAAACTCTCCATCATCGAACCTGAAGGCATACCATTGATAGACCTCTCTCCATTAAGCTCCCCAGACTCCATTTCTGACCCTAAAGCCATTGAAGTGCTTGTTAGAGAAATAGGGAATGCATGCAAGAACTGGGGCTTCTTCCAAGTGATCAACCATGGAGTGCCACTGGAAAAGCGCAAAAAGATTGATACTGCGGCCAGGAAGTTCTTTGCTCAGCCTTTGGAGGAGAAGAGAAAGATTAGGAGGGATGAAAAATGTGTGGTAGGTTACTATGACACTGAGCATACCAAAAATGTCAGAGACTGGAAGGAGGTGTTTGATTTCCTTGTGGAGGAGCCTACATTAGTACCCTCCTCGACTGAGCCTGATGACAAGGAGGAGACACAGTGGTTTAATCAATGGCCTGAGAACCCCCCTGAATTAAGGGAGGTGCTTGAAGAATATTCTCAAGAAGTAGAAAAGCTATCTCTAAAGTTGATGGGACTAATTGCGTTGAGCCTAGGCTTGCCAGAAGACAGGTTCAAAGGCTACTTCAAGGACCAAACCAGTTTTATCAGACTCAATCACTATCCACCTTGCCCTTCCCCTCAGTTAGCACTCGGTGTTGGGCGCCACAAGGATGGTGGTGCCTTAACTGTACTGTCTCAAGATGAGGTTGGAGGATTGGAGGTGAAGCGAAAAACAGATGGAGAGTGGATTCGGGTCAGACCTACACCAAATGCCTATATCATCAATGTTGGTGACATTATTCAGGTATGGAGCAACGATAAATATGAGAGTGTGGAACATAGGGTGATGGTAAACTCAGAGAAGGAGAGGTTTTCTGTTCCCTTCTTTCTTAACCCAGCACACTACACAGAAGTGAAGCCTTTGGAGGAGCTCACCAATAAACAAAACCCTGCCAAGTATAGGCCCTATAGCTGGGGCAAGTTTCTGACTCGCAGAAAGCTCAGTAATTTCAAGAAACTCAAAGCTGAAAACATCCAAATCTATCATTTCAGGGTTTCAGATCAAGCGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

356

Amino Acids

41.19

Weight (kDa)

6.0

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 26 - 153 3.1e-32 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 199 - 298 2.7e-34 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19000 AT3G19000
fragaria_vesca FvH4_2g07071 FvH4_2g07080 FvH4_2g07080 FvH4_2g07081 FvH4_2g07082 FvH4_2g07082
malus_domestica MD05G1074000.v1.1 MD10G1088100.v1.1 MD10G1088200.v1.1 MD14G1207600.v1.1
prunus_persica Prupe.8G114100_v2.0.a1 Prupe.8G114200_v2.0.a1 Prupe.8G114300_v2.0.a1 Prupe.8G114500_v2.0.a1 Prupe.8G114800_v2.0.a1 Prupe.8G115100_v2.0.a1
pyrus_communis pycom05g06640 pycom05g06660 pycom05g06670 pycom10g07050
rosa_chinensis RchiOBHm_Chr6g0244141 RchiOBHm_Chr6g0259511 RchiOBHm_Chr6g0259521 RchiOBHm_Chr6g0259531 RchiOBHm_Chr6g0259551 RchiOBHm_Chr6g0259561
rosa_laevigata RLG00000014522 RLG00000014523 RLG00000014524 RLG00000014525 RLG00000014526 RLG00000014527
rosa_multiflora Rmu_ssc0000050.1_g000019 Rmu_ssc0000050.1_g000020 Rmu_ssc0000050.1_g000026 Rmu_ssc0000050.1_g000027 Rmu_ssc0000050.1_g000028
rosa_roxburghii Rroxscaffold_7G00206780 Rroxscaffold_7G00206790 Rroxscaffold_7G00206850 Rroxscaffold_7G00206860 Rroxscaffold_7G00206870 Rroxscaffold_7G00206880
rosa_rugosa Rorug05G0589100 Rorug05G0589200.1 Rorug05G0589300 Rorug05G0589400 Rorug05G0589500 Rorug05G0589600
rosa_samantha Rh4CG096000 Rh5BG231700 Rh6AG104900 Rh6AG105000 Rh6AG105100 Rh6AG105500 Rh6AG105600 Rh6BG098500 Rh6BG098700 Rh6BG098800 Rh6BG099000 Rh6BG099100 Rh6CG094200 Rh6CG094300 Rh6CG094400 Rh6CG094500 Rh6CG094600 Rh6DG088300 Rh6DG088400 Rh6DG088500 Rh6DG088600 Rh6DG088700
rosa_wichuraiana Rw0G014270 Rw0G014280 Rw0G014290 Rw6G009080 Rw6G009090 Rw6G009100 Rw6G009110 Rw6G009120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 657, 674
AciI CCGC 1 cut(s) 242
AclWI GGATC 1 cut(s) 8
AcoI YGGCCR 1 cut(s) 243
AcuI CTGAAG 1 cut(s) 90
AcyI GRCGYC 1 cut(s) 658
AdeI CACNNNGTG 1 cut(s) 650
AfaI GTAC 2 cut(s) 396, 687
AfiI CCNNNNNNNGG 3 cut(s) 379, 957, 969
AgsI TTSAA 5 cut(s) 140, 485, 571, 583, 1009
AjnI CCWGG 1 cut(s) 245
AleI CACNNNNGTG 1 cut(s) 908
AluBI AGCT 7 cut(s) 20, 105, 511, 928, 969, 997, 1022
AluI AGCT 7 cut(s) 20, 105, 511, 928, 969, 997, 1022
Alw21I GWGCWC 1 cut(s) 930
Alw26I GTCTC 3 cut(s) 342, 422, 696
AlwI GGATC 1 cut(s) 8
AlwNI CAGNNNCTG 1 cut(s) 351
AoxI GGCC 3 cut(s) 243, 449, 959
AspA2I CCTAGG 1 cut(s) 547
AspLEI GCGC 2 cut(s) 225, 660
AspS9I GGNCC 2 cut(s) 586, 960
AsuHPI GGTGA 4 cut(s) 730, 800, 853, 922
AvaII GGWCC 1 cut(s) 586
AvrII CCTAGG 1 cut(s) 547
BaeI ACNNNNGTAYC 2 cut(s) 378, 411
BanI GGYRCC 2 cut(s) 657, 674
BanII GRGCYC 1 cut(s) 930
BbsI GAAGAC 1 cut(s) 567
Bbv12I GWGCWC 1 cut(s) 930
BccI CCATC 5 cut(s) 65, 520, 662, 728, 838
BciT130I CCWGG 1 cut(s) 247
BclI TGATCA 1 cut(s) 195
BcoDI GTCTC 3 cut(s) 342, 422, 696
BfaI CTAG 1 cut(s) 548
BfmI CTRYAG 1 cut(s) 964
BfoI RGCGCY 1 cut(s) 661
BisI GCNGC 1 cut(s) 243
BlnI CCTAGG 1 cut(s) 547
BlpI GCTNAGC 1 cut(s) 261
BlsI GCNGC 1 cut(s) 244
Bme1390I CCNGG 1 cut(s) 247
Bme18I GGWCC 1 cut(s) 586
BmgT120I GGNCC 2 cut(s) 586, 960
BmiI GGNNCC 3 cut(s) 384, 659, 676
BmrFI CCNGG 1 cut(s) 247
BmrI ACTGGG 1 cut(s) 187
BmuI ACTGGG 1 cut(s) 187
BpiI GAAGAC 1 cut(s) 567
Bpu1102I GCTNAGC 1 cut(s) 261
BpuEI CTTGAG 2 cut(s) 480, 678
BsaHI GRCGYC 1 cut(s) 658
BsaJI CCNNGG 2 cut(s) 202, 547
BsaXI ACNNNNNCTCC 2 cut(s) 419, 449
Bsc4I CCNNNNNNNGG 3 cut(s) 379, 957, 969
Bse1I ACTGG 4 cut(s) 182, 219, 356, 594
BseBI CCWGG 1 cut(s) 247
BseDI CCNNGG 2 cut(s) 202, 547
BseGI GGATG 4 cut(s) 24, 301, 673, 1029
BseLI CCNNNNNNNGG 3 cut(s) 379, 957, 969
BseMII CTCAG 7 cut(s) 275, 318, 399, 444, 650, 867, 1012
BseNI ACTGG 4 cut(s) 182, 219, 356, 594
BseRI GAGGAG 5 cut(s) 287, 391, 395, 440, 938
BseYI CCCAGC 2 cut(s) 892, 969
BshFI GGCC 3 cut(s) 245, 451, 961
BshNI GGYRCC 2 cut(s) 657, 674
BsiHKAI GWGCWC 1 cut(s) 930
BslFI GGGAC 1 cut(s) 543
BslI CCNNNNNNNGG 3 cut(s) 379, 957, 969
BsmAI GTCTC 3 cut(s) 342, 422, 696
BsmFI GGGAC 1 cut(s) 543
BsmI GAATGC 1 cut(s) 169
BsnI GGCC 3 cut(s) 245, 451, 961
Bsp1286I GDGCHC 1 cut(s) 930
Bsp143I GATC 3 cut(s) 13, 195, 1057
Bsp1720I GCTNAGC 1 cut(s) 261
Bsp19I CCATGG 1 cut(s) 202
BspACI CCGC 1 cut(s) 242
BspANI GGCC 3 cut(s) 245, 451, 961
BspCNI CTCAG 7 cut(s) 274, 319, 400, 445, 649, 866, 1011
BspLI GGNNCC 3 cut(s) 384, 659, 676
BspPI GGATC 1 cut(s) 8
BspT107I GGYRCC 2 cut(s) 657, 674
BsrI ACTGG 4 cut(s) 182, 219, 356, 594
BssECI CCNNGG 2 cut(s) 202, 547
BssMI GATC 3 cut(s) 13, 195, 1057
BssNI GRCGYC 1 cut(s) 658
BssT1I CCWWGG 2 cut(s) 202, 547
Bst2UI CCWGG 1 cut(s) 247
Bst4CI ACNGT 3 cut(s) 435, 685, 690
Bst6I CTCTTC 1 cut(s) 272
BstACI GRCGYC 1 cut(s) 658
BstC8I GCNNGC 2 cut(s) 169, 554
BstDEI CTNAG 7 cut(s) 261, 327, 408, 453, 636, 853, 998
BstDSI CCRYGG 1 cut(s) 202
BstENI CCTNNNNNAGG 1 cut(s) 377
BstF5I GGATG 4 cut(s) 24, 301, 673, 1029
BstH2I RGCGCY 1 cut(s) 661
BstHHI GCGC 2 cut(s) 225, 660
BstKTI GATC 3 cut(s) 16, 198, 1060
BstMAI GTCTC 3 cut(s) 342, 422, 696
BstMBI GATC 3 cut(s) 13, 195, 1057
BstNI CCWGG 1 cut(s) 247
BstNSI RCATGY 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 245
BstSFI CTRYAG 1 cut(s) 964
BstV2I GAAGAC 1 cut(s) 567
BstXI CCANNNNNNTGG 1 cut(s) 668
BsuRI GGCC 3 cut(s) 245, 451, 961
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 4 cut(s) 24, 301, 673, 1029
BtsIMutI CAGTG 3 cut(s) 212, 324, 440
Cac8I GCNNGC 2 cut(s) 169, 554
CaiI CAGNNNCTG 1 cut(s) 351
CfoI GCGC 2 cut(s) 225, 660
Cfr13I GGNCC 2 cut(s) 586, 960
Csp6I GTAC 2 cut(s) 395, 686
CspCI CAANNNNNGTGG 2 cut(s) 652, 687
CviAII CATG 2 cut(s) 168, 203
CviQI GTAC 2 cut(s) 395, 686
DdeI CTNAG 7 cut(s) 261, 327, 408, 453, 636, 853, 998
DinI GGCGCC 1 cut(s) 659
DpnI GATC 3 cut(s) 15, 197, 1059
DpnII GATC 3 cut(s) 13, 195, 1057
DraIII CACNNNGTG 1 cut(s) 650
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 272
EarI CTCTTC 1 cut(s) 272
Ecl136II GAGCTC 1 cut(s) 928
Eco130I CCWWGG 2 cut(s) 202, 547
Eco24I GRGCYC 1 cut(s) 930
Eco47I GGWCC 1 cut(s) 586
Eco53kI GAGCTC 1 cut(s) 928
Eco57I CTGAAG 1 cut(s) 90
EcoICRI GAGCTC 1 cut(s) 928
EcoNI CCTNNNNNAGG 1 cut(s) 377
EcoO109I RGGNCCY 1 cut(s) 960
EcoRII CCWGG 1 cut(s) 245
EcoT14I CCWWGG 2 cut(s) 202, 547
EcoT22I ATGCAT 1 cut(s) 169
EcoT38I GRGCYC 1 cut(s) 930
EgeI GGCGCC 1 cut(s) 659
EheI GGCGCC 1 cut(s) 659
ErhI CCWWGG 2 cut(s) 202, 547
FaeI CATG 2 cut(s) 171, 206
FaqI GGGAC 1 cut(s) 543
FatI CATG 2 cut(s) 167, 202
FbaI TGATCA 1 cut(s) 195
Fnu4HI GCNGC 1 cut(s) 243
FokI GGATG 4 cut(s) 11, 308, 680, 1016
FriOI GRGCYC 1 cut(s) 930
Fsp4HI GCNGC 1 cut(s) 243
FspBI CTAG 1 cut(s) 548
GlaI GCGC 2 cut(s) 224, 659
GluI GCNGC 1 cut(s) 243
GsaI CCCAGC 2 cut(s) 896, 973
HaeII RGCGCY 1 cut(s) 661
HaeIII GGCC 3 cut(s) 245, 451, 961
HhaI GCGC 2 cut(s) 225, 660
Hin1I GRCGYC 1 cut(s) 658
Hin1II CATG 2 cut(s) 171, 206
Hin6I GCGC 2 cut(s) 223, 658
HinP1I GCGC 2 cut(s) 223, 658
HinfI GANTC 4 cut(s) 113, 606, 745, 985
HphI GGTGA 4 cut(s) 730, 800, 853, 922
Hpy166II GTNNAC 1 cut(s) 850
Hpy188I TCNGA 7 cut(s) 124, 347, 605, 755, 856, 984, 1057
Hpy188III TCNNGA 3 cut(s) 497, 695, 1009
Hpy8I GTNNAC 1 cut(s) 850
HpyAV CCTTC 5 cut(s) 65, 349, 639, 853, 889
HpyCH4III ACNGT 3 cut(s) 435, 685, 690
HpyCH4V TGCA 2 cut(s) 167, 171
HpyF3I CTNAG 7 cut(s) 261, 327, 408, 453, 636, 853, 998
Hsp92I GRCGYC 1 cut(s) 658
Hsp92II CATG 2 cut(s) 171, 206
HspAI GCGC 2 cut(s) 223, 658
KasI GGCGCC 1 cut(s) 657
Ksp22I TGATCA 1 cut(s) 195
Kzo9I GATC 3 cut(s) 13, 195, 1057
LmnI GCTCC 4 cut(s) 110, 382, 807, 925
MaeI CTAG 1 cut(s) 548
MaeIII GTNAC 2 cut(s) 314, 788
MalI GATC 3 cut(s) 15, 197, 1059
MboI GATC 3 cut(s) 13, 195, 1057
MboII GAAGA 4 cut(s) 178, 289, 497, 572
MhlI GDGCHC 1 cut(s) 930
MluCI AATT 3 cut(s) 467, 534, 1003
Mly113I GGCGCC 1 cut(s) 658
MlyI GAGTC 3 cut(s) 107, 600, 979
MmeI TCCRAC 1 cut(s) 685
Mph1103I ATGCAT 1 cut(s) 169
MseI TTAA 5 cut(s) 101, 441, 470, 680, 888
MslI CAYNNNNRTG 3 cut(s) 140, 666, 908
MspR9I CCNGG 1 cut(s) 247
Mva1269I GAATGC 1 cut(s) 169
MvaI CCWGG 1 cut(s) 247
NarI GGCGCC 1 cut(s) 658
NcoI CCATGG 1 cut(s) 202
NdeII GATC 3 cut(s) 13, 195, 1057
NlaIII CATG 2 cut(s) 171, 206
NlaIV GGNNCC 3 cut(s) 384, 659, 676
NmuCI GTSAC 1 cut(s) 788
NsiI ATGCAT 1 cut(s) 169
NspI RCATGY 1 cut(s) 171
OliI CACNNNNGTG 1 cut(s) 908
PaeI GCATGC 1 cut(s) 171
PctI GAATGC 1 cut(s) 169
PfeI GAWTC 1 cut(s) 745
PkrI GCNGC 1 cut(s) 244
PleI GAGTC 3 cut(s) 107, 600, 979
PluTI GGCGCC 1 cut(s) 661
PpsI GAGTC 3 cut(s) 107, 600, 979
Psp124BI GAGCTC 1 cut(s) 930
Psp6I CCWGG 1 cut(s) 245
PspFI CCCAGC 2 cut(s) 892, 969
PspGI CCWGG 1 cut(s) 245
PspN4I GGNNCC 3 cut(s) 384, 659, 676
PspPI GGNCC 2 cut(s) 586, 960
PstNI CAGNNNCTG 1 cut(s) 351
RsaI GTAC 2 cut(s) 396, 687
RsaNI GTAC 2 cut(s) 395, 686
RseI CAYNNNNRTG 3 cut(s) 140, 666, 908
SacI GAGCTC 1 cut(s) 930
SaqAI TTAA 5 cut(s) 101, 441, 470, 680, 888
SatI GCNGC 1 cut(s) 243
Sau3AI GATC 3 cut(s) 13, 195, 1057
Sau96I GGNCC 2 cut(s) 586, 960
SchI GAGTC 3 cut(s) 107, 600, 979
ScrFI CCNGG 1 cut(s) 247
SduI GDGCHC 1 cut(s) 930
SfcI CTRYAG 1 cut(s) 964
SfoI GGCGCC 1 cut(s) 659
SinI GGWCC 1 cut(s) 586
SmiMI CAYNNNNRTG 3 cut(s) 140, 666, 908
SmlI CTYRAG 2 cut(s) 495, 693
SmoI CTYRAG 2 cut(s) 495, 693
SphI GCATGC 1 cut(s) 171
Sse9I AATT 3 cut(s) 467, 534, 1003
SsiI CCGC 1 cut(s) 242
SspDI GGCGCC 1 cut(s) 657
SspI AATATT 1 cut(s) 491
SspMI CTAG 1 cut(s) 548
SstI GAGCTC 1 cut(s) 930
StyD4I CCNGG 1 cut(s) 245
StyI CCWWGG 2 cut(s) 202, 547
TaaI ACNGT 3 cut(s) 435, 685, 690
TaqI TCGA 2 cut(s) 63, 404
TasI AATT 3 cut(s) 467, 534, 1003
TatI WGTACW 1 cut(s) 685
TauI GCSGC 1 cut(s) 245
TfiI GAWTC 1 cut(s) 745
Tru1I TTAA 5 cut(s) 101, 441, 470, 680, 888
Tru9I TTAA 5 cut(s) 101, 441, 470, 680, 888
TscAI CASTG 3 cut(s) 219, 331, 440
TseFI GTSAC 1 cut(s) 788
Tsp45I GTSAC 1 cut(s) 788
TspDTI ATGAA 2 cut(s) 13, 312
TspRI CASTG 3 cut(s) 219, 331, 440
VpaK11BI GGWCC 1 cut(s) 586
XagI CCTNNNNNAGG 1 cut(s) 377
XceI RCATGY 1 cut(s) 171
XcmI CCANNNNNNNNNTGG 1 cut(s) 668
XmaJI CCTAGG 1 cut(s) 547
XspI CTAG 1 cut(s) 548
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.