Rw6G009080

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000590
Physical Location & Seq
Reverse (-)
15288546 .. 15290070
1525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G009080.1

Sequence Viewer

Length: 1017 bp
ATGGGAGAAGTTGACCCAGCTTTCATACAACACCCTGAACACAGGCCCAAACTCTCCATAATCAAAGCCGAAGGCATACCATTAATTGACCTCTCTGCAATAAACTCTCCAAACTCCATCTCTGACCCAGAAGATATTGAAAGAATTGTTAGAGAAGTGGGCAATGCATGCAAGGAATGGGGGTTCTTCCAAGTAATCAACCATGGGGTGTCATCAGATAAGCTCCAAAAGATTGAGGCTTCTGCTAAAAAGTTCTTTGCTCTGCCTTTGGAGGAGAAGAGGAAGATTAGGAGAGACCTGAACAATGTGTTGGGGTACTATGACACTGAGAACACCAAGAATATTCGGGACTGGAAGGAGGTGTTTGATTTTGATGTGGAGGACCCTACTTTAGTCCCAGCCTCACCTGATCCTGAGGACGACGAAGAGACTGAGTGGACTAATCAATGGCCTGAGTATCCCCCAGAAATGAGGGAGGCATGTCAAGAATATGCTCGAGAAGTTGAAAAGCTAGCTCTAAAGTTGATGGGACTTATTGCCCTAAGTCTGGGCTTGCCGGAAGACAGACTCAATCACTATCCACCTTGCCCTTCCCCGGAGTTAGCCCTTGGTGTTGGTCGCCACAAGGATGGTGGCGCTCTAACTGTGCTAGCTCAAGATGATGTTGGAGGATTGGAAGTGAAGAGAAAAACAGATGGAGAGTGGGTTAGGGTAAATCCCACCCCAAATGCTTATATCATCAACCTTGGTGACACTCTTCAGGTTTGGAGCAATGAGAGATATGAGAGTACAGAACACAGGGTGATGGTGAATTCAGAGAAGGAAAGGTTTTCTATCCCGTACTTCCTTGAGCCAGCACACTACACCATAATAAAGCCCTTGGAAGAGCTGATCAATGAACAAAATCCTGCAAAGTATAGGCCATACAGCTGGGGCAAGTTTACGACTCACAGAAAGCTGAGTAATTTCAAGAAACTCAATGTTGAAAACATCCAAATTCATGATTTCAGGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

338

Amino Acids

38.93

Weight (kDa)

5.12

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 26 - 152 2.7e-33 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 189 - 285 1.2e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19000 AT3G19000
fragaria_vesca FvH4_2g07071 FvH4_2g07080 FvH4_2g07080 FvH4_2g07081 FvH4_2g07082 FvH4_2g07082
malus_domestica MD05G1074000.v1.1 MD10G1088100.v1.1 MD10G1088200.v1.1 MD14G1207600.v1.1
prunus_persica Prupe.8G114100_v2.0.a1 Prupe.8G114200_v2.0.a1 Prupe.8G114300_v2.0.a1 Prupe.8G114500_v2.0.a1 Prupe.8G114800_v2.0.a1 Prupe.8G115100_v2.0.a1
pyrus_communis pycom05g06640 pycom05g06660 pycom05g06670 pycom10g07050
rosa_chinensis RchiOBHm_Chr6g0244141 RchiOBHm_Chr6g0259511 RchiOBHm_Chr6g0259521 RchiOBHm_Chr6g0259531 RchiOBHm_Chr6g0259551 RchiOBHm_Chr6g0259561
rosa_laevigata RLG00000014522 RLG00000014523 RLG00000014524 RLG00000014525 RLG00000014526 RLG00000014527
rosa_multiflora Rmu_ssc0000050.1_g000019 Rmu_ssc0000050.1_g000020 Rmu_ssc0000050.1_g000026 Rmu_ssc0000050.1_g000027 Rmu_ssc0000050.1_g000028
rosa_roxburghii Rroxscaffold_7G00206780 Rroxscaffold_7G00206790 Rroxscaffold_7G00206850 Rroxscaffold_7G00206860 Rroxscaffold_7G00206870 Rroxscaffold_7G00206880
rosa_rugosa Rorug05G0589100 Rorug05G0589200.1 Rorug05G0589300 Rorug05G0589400 Rorug05G0589500 Rorug05G0589600
rosa_samantha Rh4CG096000 Rh5BG231700 Rh6AG104900 Rh6AG105000 Rh6AG105100 Rh6AG105500 Rh6AG105600 Rh6BG098500 Rh6BG098700 Rh6BG098800 Rh6BG099000 Rh6BG099100 Rh6CG094200 Rh6CG094300 Rh6CG094400 Rh6CG094500 Rh6CG094600 Rh6DG088300 Rh6DG088400 Rh6DG088500 Rh6DG088600 Rh6DG088700
rosa_wichuraiana Rw0G014270 Rw0G014280 Rw0G014290 Rw6G009080 Rw6G009090 Rw6G009100 Rw6G009110 Rw6G009120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 404
AcsI RAATTY 2 cut(s) 811, 996
AcuI CTGAAG 1 cut(s) 743
AdeI CACNNNGTG 1 cut(s) 802
AfaI GTAC 3 cut(s) 317, 790, 842
AfiI CCNNNNNNNGG 2 cut(s) 547, 595
AgsI TTSAA 4 cut(s) 140, 506, 970, 986
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AloI GAACNNNNNNTCC 2 cut(s) 167, 199
AluBI AGCT 8 cut(s) 20, 223, 511, 515, 653, 889, 930, 958
AluI AGCT 8 cut(s) 20, 223, 511, 515, 653, 889, 930, 958
Alw26I GTCTC 2 cut(s) 288, 422
AlwI GGATC 1 cut(s) 404
Ama87I CYCGRG 1 cut(s) 495
AoxI GGCC 3 cut(s) 44, 449, 920
ApoI RAATTY 2 cut(s) 811, 996
AseI ATTAAT 1 cut(s) 83
AspLEI GCGC 1 cut(s) 638
AspS9I GGNCC 2 cut(s) 45, 382
AsuC2I CCSGG 1 cut(s) 596
AsuHPI GGTGA 4 cut(s) 396, 761, 814, 820
AsuNHI GCTAGC 2 cut(s) 511, 649
AvaI CYCGRG 1 cut(s) 495
AvaII GGWCC 1 cut(s) 382
AxyI CCTNAGG 1 cut(s) 414
BbsI GAAGAC 1 cut(s) 567
BccI CCATC 5 cut(s) 125, 520, 623, 689, 799
BciVI GTATCC 1 cut(s) 468
BclI TGATCA 1 cut(s) 891
BcnI CCSGG 1 cut(s) 596
BcoDI GTCTC 2 cut(s) 288, 422
BfaI CTAG 2 cut(s) 512, 650
BfoI RGCGCY 1 cut(s) 639
BfuI GTATCC 1 cut(s) 468
Bme1390I CCNGG 1 cut(s) 596
Bme18I GGWCC 1 cut(s) 382
BmeT110I CYCGRG 1 cut(s) 495
BmgT120I GGNCC 2 cut(s) 45, 382
BmiI GGNNCC 1 cut(s) 384
BmrFI CCNGG 1 cut(s) 596
BmtI GCTAGC 2 cut(s) 515, 653
BpiI GAAGAC 1 cut(s) 567
BpuEI CTTGAG 2 cut(s) 639, 869
BpuMI CCSGG 1 cut(s) 596
BsaI GGTCTC 1 cut(s) 288
BsaJI CCNNGG 5 cut(s) 202, 594, 607, 745, 879
BsaXI ACNNNNNCTCC 2 cut(s) 690, 720
Bsc4I CCNNNNNNNGG 2 cut(s) 547, 595
Bse1I ACTGG 1 cut(s) 356
Bse21I CCTNAGG 1 cut(s) 414
Bse3DI GCAATG 2 cut(s) 169, 778
BseDI CCNNGG 5 cut(s) 202, 594, 607, 745, 879
BseGI GGATG 2 cut(s) 634, 990
BseLI CCNNNNNNNGG 2 cut(s) 547, 595
BseMI GCAATG 2 cut(s) 169, 778
BseMII CTCAG 5 cut(s) 318, 405, 423, 444, 950
BseNI ACTGG 1 cut(s) 356
BseRI GAGGAG 1 cut(s) 287
BseYI CCCAGC 3 cut(s) 16, 397, 930
BshFI GGCC 3 cut(s) 46, 451, 922
BsiHKCI CYCGRG 1 cut(s) 495
BsiSI CCGG 2 cut(s) 557, 596
BslFI GGGAC 3 cut(s) 362, 380, 543
BslI CCNNNNNNNGG 2 cut(s) 547, 595
BsmAI GTCTC 2 cut(s) 288, 422
BsmFI GGGAC 3 cut(s) 362, 380, 543
BsnI GGCC 3 cut(s) 46, 451, 922
Bso31I GGTCTC 1 cut(s) 288
BsoBI CYCGRG 1 cut(s) 495
Bsp143I GATC 2 cut(s) 409, 891
Bsp19I CCATGG 1 cut(s) 202
BspANI GGCC 3 cut(s) 46, 451, 922
BspCNI CTCAG 5 cut(s) 319, 406, 424, 445, 951
BspHI TCATGA 1 cut(s) 1000
BspLI GGNNCC 1 cut(s) 384
BspOI GCTAGC 2 cut(s) 515, 653
BspPI GGATC 1 cut(s) 404
BspQI GCTCTTC 1 cut(s) 879
BspTNI GGTCTC 1 cut(s) 288
BsrDI GCAATG 2 cut(s) 169, 778
BsrI ACTGG 1 cut(s) 356
BssECI CCNNGG 5 cut(s) 202, 594, 607, 745, 879
BssMI GATC 2 cut(s) 409, 891
BssT1I CCWWGG 4 cut(s) 202, 607, 745, 879
Bst4CI ACNGT 1 cut(s) 646
Bst6I CTCTTC 5 cut(s) 272, 420, 677, 762, 879
BstAPI GCANNNNNTGC 1 cut(s) 168
BstC8I GCNNGC 5 cut(s) 169, 513, 554, 651, 855
BstDEI CTNAG 6 cut(s) 327, 414, 432, 453, 542, 959
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 2 cut(s) 634, 990
BstH2I RGCGCY 1 cut(s) 639
BstHHI GCGC 1 cut(s) 638
BstKTI GATC 2 cut(s) 412, 894
BstMAI GTCTC 2 cut(s) 288, 422
BstMBI GATC 2 cut(s) 409, 891
BstMWI GCNNNNNNNGC 1 cut(s) 168
BstNSI RCATGY 2 cut(s) 171, 483
BstSCI CCNGG 1 cut(s) 594
BstV2I GAAGAC 1 cut(s) 567
BstXI CCANNNNNNTGG 2 cut(s) 629, 930
Bsu36I CCTNAGG 1 cut(s) 414
BsuI GTATCC 1 cut(s) 468
BsuRI GGCC 3 cut(s) 46, 451, 922
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 2 cut(s) 634, 990
BtsIMutI CAGTG 1 cut(s) 324
Cac8I GCNNGC 5 cut(s) 169, 513, 554, 651, 855
CciI TCATGA 1 cut(s) 1000
CfoI GCGC 1 cut(s) 638
Cfr13I GGNCC 2 cut(s) 45, 382
Csp6I GTAC 3 cut(s) 316, 789, 841
CspCI CAANNNNNGTGG 2 cut(s) 613, 648
CviAII CATG 4 cut(s) 168, 203, 480, 1001
CviQI GTAC 3 cut(s) 316, 789, 841
DdeI CTNAG 6 cut(s) 327, 414, 432, 453, 542, 959
DpnI GATC 2 cut(s) 411, 893
DpnII GATC 2 cut(s) 409, 891
DraIII CACNNNGTG 1 cut(s) 802
Eam1104I CTCTTC 5 cut(s) 272, 420, 677, 762, 879
EarI CTCTTC 5 cut(s) 272, 420, 677, 762, 879
Eco130I CCWWGG 4 cut(s) 202, 607, 745, 879
Eco31I GGTCTC 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 743
Eco81I CCTNAGG 1 cut(s) 414
Eco88I CYCGRG 1 cut(s) 495
EcoO109I RGGNCCY 1 cut(s) 382
EcoRI GAATTC 1 cut(s) 811
EcoT14I CCWWGG 4 cut(s) 202, 607, 745, 879
EcoT22I ATGCAT 1 cut(s) 169
ErhI CCWWGG 4 cut(s) 202, 607, 745, 879
FaeI CATG 4 cut(s) 171, 206, 483, 1004
FaqI GGGAC 3 cut(s) 362, 380, 543
FatI CATG 4 cut(s) 167, 202, 479, 1000
FbaI TGATCA 1 cut(s) 891
FokI GGATG 2 cut(s) 641, 977
FspBI CTAG 2 cut(s) 512, 650
GlaI GCGC 1 cut(s) 637
GsaI CCCAGC 3 cut(s) 20, 401, 934
HaeII RGCGCY 1 cut(s) 639
HaeIII GGCC 3 cut(s) 46, 451, 922
HapII CCGG 2 cut(s) 557, 596
HhaI GCGC 1 cut(s) 638
Hin1II CATG 4 cut(s) 171, 206, 483, 1004
Hin6I GCGC 1 cut(s) 636
HinP1I GCGC 1 cut(s) 636
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 2 cut(s) 567, 946
HpaII CCGG 2 cut(s) 557, 596
HphI GGTGA 4 cut(s) 396, 761, 814, 820
Hpy166II GTNNAC 3 cut(s) 13, 438, 942
Hpy188I TCNGA 3 cut(s) 124, 217, 817
Hpy188III TCNNGA 8 cut(s) 347, 413, 485, 497, 656, 970, 1001, 1009
Hpy8I GTNNAC 3 cut(s) 13, 438, 942
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 4 cut(s) 65, 349, 600, 814
HpyCH4III ACNGT 1 cut(s) 646
HpyCH4V TGCA 4 cut(s) 98, 167, 171, 911
HpyF10VI GCNNNNNNNGC 1 cut(s) 168
HpyF3I CTNAG 6 cut(s) 327, 414, 432, 453, 542, 959
Hsp92II CATG 4 cut(s) 171, 206, 483, 1004
HspAI GCGC 1 cut(s) 636
Ksp22I TGATCA 1 cut(s) 891
Kzo9I GATC 2 cut(s) 409, 891
LguI GCTCTTC 1 cut(s) 879
LmnI GCTCC 2 cut(s) 228, 768
MaeI CTAG 2 cut(s) 512, 650
MaeIII GTNAC 1 cut(s) 749
MalI GATC 2 cut(s) 411, 893
MboI GATC 2 cut(s) 409, 891
MboII GAAGA 9 cut(s) 143, 178, 289, 295, 437, 572, 694, 749, 896
MluCI AATT 5 cut(s) 84, 144, 811, 964, 996
MlyI GAGTC 2 cut(s) 561, 940
MmeI TCCRAC 1 cut(s) 646
Mph1103I ATGCAT 1 cut(s) 169
MseI TTAA 1 cut(s) 83
MslI CAYNNNNRTG 1 cut(s) 627
MspA1I CMGCKG 1 cut(s) 930
MspI CCGG 2 cut(s) 557, 596
MspR9I CCNGG 1 cut(s) 596
MwoI GCNNNNNNNGC 1 cut(s) 168
NciI CCSGG 1 cut(s) 596
NcoI CCATGG 1 cut(s) 202
NdeII GATC 2 cut(s) 409, 891
NheI GCTAGC 2 cut(s) 511, 649
NlaIII CATG 4 cut(s) 171, 206, 483, 1004
NlaIV GGNNCC 1 cut(s) 384
NmuCI GTSAC 1 cut(s) 749
NsiI ATGCAT 1 cut(s) 169
NspI RCATGY 2 cut(s) 171, 483
PaeI GCATGC 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 495
PagI TCATGA 1 cut(s) 1000
PciSI GCTCTTC 1 cut(s) 879
PleI GAGTC 2 cut(s) 561, 940
PpsI GAGTC 2 cut(s) 561, 940
PpuMI RGGWCCY 1 cut(s) 382
PshBI ATTAAT 1 cut(s) 83
Psp5II RGGWCCY 1 cut(s) 382
PspFI CCCAGC 3 cut(s) 16, 397, 930
PspN4I GGNNCC 1 cut(s) 384
PspPI GGNCC 2 cut(s) 45, 382
PspPPI RGGWCCY 1 cut(s) 382
PvuII CAGCTG 1 cut(s) 930
RsaI GTAC 3 cut(s) 317, 790, 842
RsaNI GTAC 3 cut(s) 316, 789, 841
RseI CAYNNNNRTG 1 cut(s) 627
SapI GCTCTTC 1 cut(s) 879
SaqAI TTAA 1 cut(s) 83
Sau3AI GATC 2 cut(s) 409, 891
Sau96I GGNCC 2 cut(s) 45, 382
SchI GAGTC 2 cut(s) 561, 940
ScrFI CCNGG 1 cut(s) 596
Sfr274I CTCGAG 1 cut(s) 495
SinI GGWCC 1 cut(s) 382
SlaI CTCGAG 1 cut(s) 495
SmiMI CAYNNNNRTG 1 cut(s) 627
SmlI CTYRAG 3 cut(s) 495, 654, 848
SmoI CTYRAG 3 cut(s) 495, 654, 848
SphI GCATGC 1 cut(s) 171
Sse9I AATT 5 cut(s) 84, 144, 811, 964, 996
SspI AATATT 1 cut(s) 343
SspMI CTAG 2 cut(s) 512, 650
StyD4I CCNGG 1 cut(s) 594
StyI CCWWGG 4 cut(s) 202, 607, 745, 879
TaaI ACNGT 1 cut(s) 646
TaqI TCGA 1 cut(s) 496
TasI AATT 5 cut(s) 84, 144, 811, 964, 996
TatI WGTACW 1 cut(s) 788
Tru1I TTAA 1 cut(s) 83
Tru9I TTAA 1 cut(s) 83
TscAI CASTG 1 cut(s) 331
TseFI GTSAC 1 cut(s) 749
Tsp45I GTSAC 1 cut(s) 749
TspDTI ATGAA 3 cut(s) 13, 912, 989
TspRI CASTG 1 cut(s) 331
VpaK11BI GGWCC 1 cut(s) 382
VspI ATTAAT 1 cut(s) 83
XapI RAATTY 2 cut(s) 811, 996
XceI RCATGY 2 cut(s) 171, 483
XcmI CCANNNNNNNNNTGG 1 cut(s) 629
XhoI CTCGAG 1 cut(s) 495
XspI CTAG 2 cut(s) 512, 650
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.