Rw0G014280

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00641
OG0000590
Physical Location & Seq
Reverse (-)
4391 .. 5690
1300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G014280.1

Sequence Viewer

Length: 954 bp
ATGGGAGAGGTTGATCCAGCTTTCATCCAAGATCCTGAGCACAGGCCGAAACATCCTCATCATCGAATAACCTTGCAACGATTTCATTCCTACCCCAAATCCATTGAAGGTGTTGTCAGAGAAATAGGGAATGCATGCAAGGAGTGGGGGTTCTTCCAAGTGATCAACCATGGGGCTGCTGCTAGGAAGTTCTTTGCACAGCCTCTGGAGGATAAGAGGAAGATCAGGAGAGATGAAAGAAACGCATTGGGGTACTTTGACACCGAGAACACTACTTTAGTCCCAGCTTCAGCTGATCCCGAGGACAAGGAAGAGGCAACATGGACTAACCAATGGCCTGAGTATCCTCCAGAATTAAGGGAAGCATGTGAAGAATATACTCAGGAAGTAGAAAAGCTTGCTCTAAAGTTATTAGGACTTATAGCCAGGAGTCTAGGCTTGCCAGGAAACAGGTTCAACAGCTACTTCAAAGACCAAAGCACTCGTATCAGACTCAATCACTATCCCCCTTGCCCTTCCCCTCAGTTAGCTCTTGGTGTTGGTCGCCACAAGGATGGTGGTGCTTTAACCGTCCTAGCTCAAGATGAGGTTGGTGGATTGGAAGTGAAGAGAAAGACAGATGGAGAGTGGATTCGGGTTAAACCTACACCAAATGCCTATATCATCAATGTTGGCGATATTATGCAGGTTTGGAGCAATGATAGATATGAGAGTGTTGAGCACAGGGTGATGGTGGACTCAGAGAAGGAAAGGTTTTCCATTCCTTTCTTCTTTAATCCAGCACACTACACACTAGTGAAGCCTGTGGAGGAGCTGATCGATGAACAAAACCCTGCCAAGTACAGGCCTTACAGCTGGGGAAAGTTTATGAGAAAGTTTATGACTCACAGAAGACTCAGTAATTTCAAGAAGCTCGGTGTTGACAACATCCAAATATATGATTTTAGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

317

Amino Acids

36.9

Weight (kDa)

7.75

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 24 - 101 1.4e-12 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 163 - 260 2.2e-34 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19000 AT3G19000
fragaria_vesca FvH4_2g07071 FvH4_2g07080 FvH4_2g07080 FvH4_2g07081 FvH4_2g07082 FvH4_2g07082
malus_domestica MD05G1074000.v1.1 MD10G1088100.v1.1 MD10G1088200.v1.1 MD14G1207600.v1.1
prunus_persica Prupe.8G114100_v2.0.a1 Prupe.8G114200_v2.0.a1 Prupe.8G114300_v2.0.a1 Prupe.8G114500_v2.0.a1 Prupe.8G114800_v2.0.a1 Prupe.8G115100_v2.0.a1
pyrus_communis pycom05g06640 pycom05g06660 pycom05g06670 pycom10g07050
rosa_chinensis RchiOBHm_Chr6g0244141 RchiOBHm_Chr6g0259511 RchiOBHm_Chr6g0259521 RchiOBHm_Chr6g0259531 RchiOBHm_Chr6g0259551 RchiOBHm_Chr6g0259561
rosa_laevigata RLG00000014522 RLG00000014523 RLG00000014524 RLG00000014525 RLG00000014526 RLG00000014527
rosa_multiflora Rmu_ssc0000050.1_g000019 Rmu_ssc0000050.1_g000020 Rmu_ssc0000050.1_g000026 Rmu_ssc0000050.1_g000027 Rmu_ssc0000050.1_g000028
rosa_roxburghii Rroxscaffold_7G00206780 Rroxscaffold_7G00206790 Rroxscaffold_7G00206850 Rroxscaffold_7G00206860 Rroxscaffold_7G00206870 Rroxscaffold_7G00206880
rosa_rugosa Rorug05G0589100 Rorug05G0589200.1 Rorug05G0589300 Rorug05G0589400 Rorug05G0589500 Rorug05G0589600
rosa_samantha Rh4CG096000 Rh5BG231700 Rh6AG104900 Rh6AG105000 Rh6AG105100 Rh6AG105500 Rh6AG105600 Rh6BG098500 Rh6BG098700 Rh6BG098800 Rh6BG099000 Rh6BG099100 Rh6CG094200 Rh6CG094300 Rh6CG094400 Rh6CG094500 Rh6CG094600 Rh6DG088300 Rh6DG088400 Rh6DG088500 Rh6DG088600 Rh6DG088700
rosa_wichuraiana Rw0G014270 Rw0G014280 Rw0G014290 Rw6G009080 Rw6G009090 Rw6G009100 Rw6G009110 Rw6G009120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 676
AclWI GGATC 3 cut(s) 8, 26, 290
AcuI CTGAAG 1 cut(s) 273
AdeI CACNNNGTG 1 cut(s) 727
AfaI GTAC 2 cut(s) 254, 842
AfiI CCNNNNNNNGG 1 cut(s) 843
AgsI TTSAA 4 cut(s) 107, 457, 469, 907
AhlI ACTAGT 1 cut(s) 793
AjnI CCWGG 2 cut(s) 425, 442
AleI CACNNNNGTG 1 cut(s) 794
AloI GAACNNNNNNTCC 2 cut(s) 134, 166
Alw21I GWGCWC 2 cut(s) 42, 723
AlwI GGATC 3 cut(s) 8, 26, 290
AlwNI CAGNNNCTG 1 cut(s) 205
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 3 cut(s) 44, 335, 845
ApeKI GCWGC 2 cut(s) 176, 179
AsuHPI GGTGA 1 cut(s) 739
AvaI CYCGRG 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 898
Bbv12I GWGCWC 2 cut(s) 42, 723
BbvI GCAGC 2 cut(s) 163, 166
BccI CCATC 3 cut(s) 548, 614, 724
BciT130I CCWGG 2 cut(s) 427, 444
BciVI GTATCC 1 cut(s) 354
BclI TGATCA 1 cut(s) 162
BcuI ACTAGT 1 cut(s) 793
BfaI CTAG 4 cut(s) 183, 434, 575, 794
BfuAI ACCTGC 1 cut(s) 676
BfuI GTATCC 1 cut(s) 354
BisI GCNGC 2 cut(s) 177, 180
BlsI GCNGC 2 cut(s) 178, 181
Bme1390I CCNGG 2 cut(s) 427, 444
BmeT110I CYCGRG 1 cut(s) 299
BmrFI CCNGG 2 cut(s) 427, 444
BpiI GAAGAC 1 cut(s) 898
BpmI CTGGAG 2 cut(s) 227, 333
Bpu10I CCTNAGC 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 564
Bsa29I ATCGAT 1 cut(s) 819
BsaJI CCNNGG 2 cut(s) 169, 300
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 1 cut(s) 843
Bse3DI GCAATG 1 cut(s) 703
BseBI CCWGG 2 cut(s) 427, 444
BseCI ATCGAT 1 cut(s) 819
BseDI CCNNGG 2 cut(s) 169, 300
BseGI GGATG 4 cut(s) 24, 52, 559, 927
BseLI CCNNNNNNNGG 1 cut(s) 843
BseMI GCAATG 1 cut(s) 703
BseMII CTCAG 6 cut(s) 27, 330, 395, 536, 753, 910
BseRI GAGGAG 1 cut(s) 824
BseXI GCAGC 2 cut(s) 163, 166
BseYI CCCAGC 2 cut(s) 283, 855
BshFI GGCC 3 cut(s) 46, 337, 847
BshVI ATCGAT 1 cut(s) 819
BsiHKAI GWGCWC 2 cut(s) 42, 723
BsiHKCI CYCGRG 1 cut(s) 299
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 1 cut(s) 843
BsmFI GGGAC 1 cut(s) 266
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 3 cut(s) 46, 337, 847
BsoBI CYCGRG 1 cut(s) 299
Bsp1286I GDGCHC 2 cut(s) 42, 723
Bsp143I GATC 6 cut(s) 13, 31, 162, 222, 295, 816
Bsp19I CCATGG 1 cut(s) 169
BspANI GGCC 3 cut(s) 46, 337, 847
BspCNI CTCAG 6 cut(s) 28, 331, 394, 535, 752, 909
BspDI ATCGAT 1 cut(s) 819
BspMI ACCTGC 1 cut(s) 676
BspPI GGATC 3 cut(s) 8, 26, 290
BsrDI GCAATG 1 cut(s) 703
BssECI CCNNGG 2 cut(s) 169, 300
BssMI GATC 6 cut(s) 13, 31, 162, 222, 295, 816
BssT1I CCWWGG 1 cut(s) 169
Bst2UI CCWGG 2 cut(s) 427, 444
Bst4CI ACNGT 1 cut(s) 571
Bst6I CTCTTC 2 cut(s) 306, 602
BstC8I GCNNGC 3 cut(s) 136, 399, 440
BstDEI CTNAG 6 cut(s) 36, 339, 381, 522, 739, 896
BstDSI CCRYGG 1 cut(s) 169
BstF5I GGATG 4 cut(s) 24, 52, 559, 927
BstKTI GATC 6 cut(s) 16, 34, 165, 225, 298, 819
BstMBI GATC 6 cut(s) 13, 31, 162, 222, 295, 816
BstNI CCWGG 2 cut(s) 427, 444
BstNSI RCATGY 2 cut(s) 138, 369
BstSCI CCNGG 2 cut(s) 425, 442
BstV1I GCAGC 2 cut(s) 163, 166
BstV2I GAAGAC 1 cut(s) 898
BstX2I RGATCY 1 cut(s) 31
BstXI CCANNNNNNTGG 1 cut(s) 554
BstYI RGATCY 1 cut(s) 31
Bsu15I ATCGAT 1 cut(s) 819
BsuI GTATCC 1 cut(s) 354
BsuRI GGCC 3 cut(s) 46, 337, 847
BsuTUI ATCGAT 1 cut(s) 819
BtgI CCRYGG 1 cut(s) 169
BtsCI GGATG 4 cut(s) 24, 52, 559, 927
BveI ACCTGC 1 cut(s) 676
Cac8I GCNNGC 3 cut(s) 136, 399, 440
CaiI CAGNNNCTG 1 cut(s) 205
ClaI ATCGAT 1 cut(s) 819
Csp6I GTAC 2 cut(s) 253, 841
CspCI CAANNNNNGTGG 2 cut(s) 538, 573
CviAII CATG 4 cut(s) 135, 170, 321, 366
CviQI GTAC 2 cut(s) 253, 841
DdeI CTNAG 6 cut(s) 36, 339, 381, 522, 739, 896
DpnI GATC 6 cut(s) 15, 33, 164, 224, 297, 818
DpnII GATC 6 cut(s) 13, 31, 162, 222, 295, 816
DraIII CACNNNGTG 1 cut(s) 727
Eam1104I CTCTTC 2 cut(s) 306, 602
EarI CTCTTC 2 cut(s) 306, 602
Eco130I CCWWGG 1 cut(s) 169
Eco147I AGGCCT 1 cut(s) 847
Eco57I CTGAAG 1 cut(s) 273
Eco88I CYCGRG 1 cut(s) 299
EcoRII CCWGG 2 cut(s) 425, 442
EcoT14I CCWWGG 1 cut(s) 169
EcoT22I ATGCAT 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 169
FaeI CATG 4 cut(s) 138, 173, 324, 369
FaqI GGGAC 1 cut(s) 266
FatI CATG 4 cut(s) 134, 169, 320, 365
FbaI TGATCA 1 cut(s) 162
Fnu4HI GCNGC 2 cut(s) 177, 180
FokI GGATG 4 cut(s) 11, 39, 566, 914
Fsp4HI GCNGC 2 cut(s) 177, 180
FspBI CTAG 4 cut(s) 183, 434, 575, 794
GluI GCNGC 2 cut(s) 177, 180
GsaI CCCAGC 2 cut(s) 287, 859
GsuI CTGGAG 2 cut(s) 227, 333
HaeIII GGCC 3 cut(s) 46, 337, 847
Hin1II CATG 4 cut(s) 138, 173, 324, 369
HincII GTYRAC 1 cut(s) 922
HindII GTYRAC 1 cut(s) 922
HindIII AAGCTT 1 cut(s) 395
HinfI GANTC 6 cut(s) 430, 492, 631, 737, 883, 894
HphI GGTGA 1 cut(s) 739
Hpy166II GTNNAC 2 cut(s) 736, 922
Hpy188I TCNGA 3 cut(s) 119, 491, 742
Hpy188III TCNNGA 8 cut(s) 35, 206, 226, 299, 350, 383, 581, 907
Hpy8I GTNNAC 2 cut(s) 736, 922
HpyAV CCTTC 3 cut(s) 101, 525, 739
HpyCH4III ACNGT 1 cut(s) 571
HpyCH4V TGCA 5 cut(s) 76, 134, 138, 197, 685
HpyF3I CTNAG 6 cut(s) 36, 339, 381, 522, 739, 896
Hsp92II CATG 4 cut(s) 138, 173, 324, 369
Ksp22I TGATCA 1 cut(s) 162
Kzo9I GATC 6 cut(s) 13, 31, 162, 222, 295, 816
LmnI GCTCC 2 cut(s) 693, 811
Lsp1109I GCAGC 2 cut(s) 163, 166
MaeI CTAG 4 cut(s) 183, 434, 575, 794
MalI GATC 6 cut(s) 15, 33, 164, 224, 297, 818
MboI GATC 6 cut(s) 13, 31, 162, 222, 295, 816
MboII GAAGA 7 cut(s) 145, 232, 323, 383, 619, 760, 903
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 2 cut(s) 42, 723
MluCI AATT 2 cut(s) 353, 901
MlyI GAGTC 5 cut(s) 439, 486, 731, 877, 888
Mph1103I ATGCAT 1 cut(s) 136
MseI TTAA 4 cut(s) 356, 566, 639, 774
MslI CAYNNNNRTG 2 cut(s) 552, 794
MspA1I CMGCKG 2 cut(s) 293, 855
MspR9I CCNGG 2 cut(s) 427, 444
Mva1269I GAATGC 1 cut(s) 136
MvaI CCWGG 2 cut(s) 427, 444
NcoI CCATGG 1 cut(s) 169
NdeII GATC 6 cut(s) 13, 31, 162, 222, 295, 816
NlaIII CATG 4 cut(s) 138, 173, 324, 369
NsiI ATGCAT 1 cut(s) 136
NspI RCATGY 2 cut(s) 138, 369
OliI CACNNNNGTG 1 cut(s) 794
PaeI GCATGC 1 cut(s) 138
PceI AGGCCT 1 cut(s) 847
PctI GAATGC 1 cut(s) 136
PfeI GAWTC 1 cut(s) 631
PkrI GCNGC 2 cut(s) 178, 181
PleI GAGTC 5 cut(s) 438, 486, 731, 877, 888
PpsI GAGTC 5 cut(s) 438, 486, 731, 877, 888
Psp6I CCWGG 2 cut(s) 425, 442
PspFI CCCAGC 2 cut(s) 283, 855
PspGI CCWGG 2 cut(s) 425, 442
PstNI CAGNNNCTG 1 cut(s) 205
PsuI RGATCY 1 cut(s) 31
PvuII CAGCTG 2 cut(s) 293, 855
RsaI GTAC 2 cut(s) 254, 842
RsaNI GTAC 2 cut(s) 253, 841
RseI CAYNNNNRTG 2 cut(s) 552, 794
SaqAI TTAA 4 cut(s) 356, 566, 639, 774
SatI GCNGC 2 cut(s) 177, 180
Sau3AI GATC 6 cut(s) 13, 31, 162, 222, 295, 816
SchI GAGTC 5 cut(s) 439, 486, 731, 877, 888
ScrFI CCNGG 2 cut(s) 427, 444
SduI GDGCHC 2 cut(s) 42, 723
SmiMI CAYNNNNRTG 2 cut(s) 552, 794
SmlI CTYRAG 1 cut(s) 579
SmoI CTYRAG 1 cut(s) 579
SpeI ACTAGT 1 cut(s) 793
SphI GCATGC 1 cut(s) 138
Sse9I AATT 2 cut(s) 353, 901
SseBI AGGCCT 1 cut(s) 847
SspMI CTAG 4 cut(s) 183, 434, 575, 794
StuI AGGCCT 1 cut(s) 847
StyD4I CCNGG 2 cut(s) 425, 442
StyI CCWWGG 1 cut(s) 169
TaaI ACNGT 1 cut(s) 571
TaqI TCGA 2 cut(s) 64, 819
TasI AATT 2 cut(s) 353, 901
TatI WGTACW 1 cut(s) 840
TfiI GAWTC 1 cut(s) 631
Tru1I TTAA 4 cut(s) 356, 566, 639, 774
Tru9I TTAA 4 cut(s) 356, 566, 639, 774
TseI GCWGC 2 cut(s) 176, 179
TspDTI ATGAA 4 cut(s) 13, 74, 249, 837
XceI RCATGY 2 cut(s) 138, 369
XcmI CCANNNNNNNNNTGG 1 cut(s) 554
XspI CTAG 4 cut(s) 183, 434, 575, 794
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.