Rroxscaffold_7G00206780

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000590
Physical Location & Seq
Reverse (-)
56523359 .. 56525935
2577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00206780.1

Sequence Viewer

Length: 921 bp
ATGGAAGACACTACTCTAGTCCCAGCTTCAGCTGTTCCCAAGAACAAGGAAGAGGCAACATGGACTAACCAATGGCCTGAGTATCCTCCAGAATTAAGGGAAGCATGTGAAGAATATACTCAGGAAGTAGAGAAACTTGCTCTGAAGTTATTAGGACTTATAGCCAGGAGCCTAGGCTTGCCAGAAAACAGAGAAGTGGGCAATGCATGCAAGGAATGGGGGTTCTTCCAAGTAATCAACCATGGGGTGTCATCAGATAAGCTCCAAAAGATTGAGGCTTCTGCTAAAAAGTTCTTTGCTCTGCCTTTGGAGGAGAAGAGGAAGATTAGGAGAGACCTGAACAATGTGTTGGGGTACTTTGACACTGAGAACACCAAGAATATTCGGGACTGGAAAGAGGGGTTTGACTTTGATGTGGAGGACCCTACTTTAACTGCCATGACTAGGGAGGCATGTCAAGAATATGCTAGAGAAGTTGAAAAGCTTGCTCTAAAGTTGATGGGACTTATTGCCTTAAGTCTGGGCTTGCCAAAAGACAGGTTCAAAGGCTACTTCAAAGACCAAACCTCTCGTATCAGACTCAATCACTATCCACCTTTCCCTTCCCCGGAGTTAGCCCTTGGTGTTGGTCGCCACAAGGATGGTGGCGCTCTAACTGTGCTAGCTCAACATGATGTTGGAGGATTGGAAGTGAAGAGAAAAACAGATGGAGAGTGGGTTAGGGTTTGGAGCAATGAGAGATATGAGAGTACAGAACACAGGGTGATGGTGAATTCAGAGAAGGAAAGGTTTTCTATCCCGTACTTCCTTGAGCCAGCACACTACACCATAATAAAGCCCTTGGAAGAGCTGATCATGAACAAAATCTTGCAAAGTATAGGCCATACAGCTGGGGCAAGTTTACGACTCACAGAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

306

Amino Acids

35.02

Weight (kDa)

5.92

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 64 - 143 9.4e-26 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 191 - 272 4.8e-18 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19000 AT3G19000
fragaria_vesca FvH4_2g07071 FvH4_2g07080 FvH4_2g07080 FvH4_2g07081 FvH4_2g07082 FvH4_2g07082
malus_domestica MD05G1074000.v1.1 MD10G1088100.v1.1 MD10G1088200.v1.1 MD14G1207600.v1.1
prunus_persica Prupe.8G114100_v2.0.a1 Prupe.8G114200_v2.0.a1 Prupe.8G114300_v2.0.a1 Prupe.8G114500_v2.0.a1 Prupe.8G114800_v2.0.a1 Prupe.8G115100_v2.0.a1
pyrus_communis pycom05g06640 pycom05g06660 pycom05g06670 pycom10g07050
rosa_chinensis RchiOBHm_Chr6g0244141 RchiOBHm_Chr6g0259511 RchiOBHm_Chr6g0259521 RchiOBHm_Chr6g0259531 RchiOBHm_Chr6g0259551 RchiOBHm_Chr6g0259561
rosa_laevigata RLG00000014522 RLG00000014523 RLG00000014524 RLG00000014525 RLG00000014526 RLG00000014527
rosa_multiflora Rmu_ssc0000050.1_g000019 Rmu_ssc0000050.1_g000020 Rmu_ssc0000050.1_g000026 Rmu_ssc0000050.1_g000027 Rmu_ssc0000050.1_g000028
rosa_roxburghii Rroxscaffold_7G00206780 Rroxscaffold_7G00206790 Rroxscaffold_7G00206850 Rroxscaffold_7G00206860 Rroxscaffold_7G00206870 Rroxscaffold_7G00206880
rosa_rugosa Rorug05G0589100 Rorug05G0589200.1 Rorug05G0589300 Rorug05G0589400 Rorug05G0589500 Rorug05G0589600
rosa_samantha Rh4CG096000 Rh5BG231700 Rh6AG104900 Rh6AG105000 Rh6AG105100 Rh6AG105500 Rh6AG105600 Rh6BG098500 Rh6BG098700 Rh6BG098800 Rh6BG099000 Rh6BG099100 Rh6CG094200 Rh6CG094300 Rh6CG094400 Rh6CG094500 Rh6CG094600 Rh6DG088300 Rh6DG088400 Rh6DG088500 Rh6DG088600 Rh6DG088700
rosa_wichuraiana Rw0G014270 Rw0G014280 Rw0G014290 Rw6G009080 Rw6G009090 Rw6G009100 Rw6G009110 Rw6G009120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 772
AcuI CTGAAG 2 cut(s) 12, 164
AdeI CACNNNGTG 1 cut(s) 763
AfaI GTAC 3 cut(s) 356, 751, 803
AfiI CCNNNNNNNGG 2 cut(s) 444, 607
AflII CTTAAG 1 cut(s) 514
AgsI TTSAA 3 cut(s) 479, 544, 556
AjnI CCWGG 1 cut(s) 164
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
AloI GAACNNNNNNTCC 2 cut(s) 206, 238
AluBI AGCT 8 cut(s) 26, 32, 262, 484, 665, 850, 890, 918
AluI AGCT 8 cut(s) 26, 32, 262, 484, 665, 850, 890, 918
Alw26I GTCTC 1 cut(s) 327
AoxI GGCC 2 cut(s) 74, 880
ApoI RAATTY 1 cut(s) 772
AspA2I CCTAGG 1 cut(s) 172
AspLEI GCGC 1 cut(s) 650
AspS9I GGNCC 1 cut(s) 421
AsuC2I CCSGG 1 cut(s) 608
AsuHPI GGTGA 2 cut(s) 775, 781
AsuNHI GCTAGC 1 cut(s) 661
AvaII GGWCC 1 cut(s) 421
AvrII CCTAGG 1 cut(s) 172
BbsI GAAGAC 1 cut(s) 12
BccI CCATC 4 cut(s) 493, 635, 701, 760
BciT130I CCWGG 1 cut(s) 166
BciVI GTATCC 1 cut(s) 93
BclI TGATCA 1 cut(s) 852
BcnI CCSGG 1 cut(s) 608
BcoDI GTCTC 1 cut(s) 327
BfaI CTAG 5 cut(s) 17, 173, 444, 468, 662
BfoI RGCGCY 1 cut(s) 651
BfrI CTTAAG 1 cut(s) 514
BfuI GTATCC 1 cut(s) 93
BlnI CCTAGG 1 cut(s) 172
Bme1390I CCNGG 2 cut(s) 166, 608
Bme18I GGWCC 1 cut(s) 421
BmgT120I GGNCC 1 cut(s) 421
BmiI GGNNCC 2 cut(s) 170, 423
BmrFI CCNGG 2 cut(s) 166, 608
BmtI GCTAGC 1 cut(s) 665
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 830
BpuMI CCSGG 1 cut(s) 608
BsaI GGTCTC 1 cut(s) 327
BsaJI CCNNGG 5 cut(s) 172, 241, 606, 619, 840
BsaXI ACNNNNNCTCC 2 cut(s) 702, 732
Bsc4I CCNNNNNNNGG 2 cut(s) 444, 607
Bse1I ACTGG 1 cut(s) 395
Bse3DI GCAATG 2 cut(s) 208, 739
BseBI CCWGG 1 cut(s) 166
BseDI CCNNGG 5 cut(s) 172, 241, 606, 619, 840
BseGI GGATG 1 cut(s) 646
BseLI CCNNNNNNNGG 2 cut(s) 444, 607
BseMI GCAATG 2 cut(s) 208, 739
BseMII CTCAG 3 cut(s) 69, 134, 357
BseNI ACTGG 1 cut(s) 395
BseRI GAGGAG 1 cut(s) 326
BseYI CCCAGC 2 cut(s) 22, 890
BshFI GGCC 2 cut(s) 76, 882
BsiSI CCGG 1 cut(s) 608
BslFI GGGAC 3 cut(s) 5, 401, 516
BslI CCNNNNNNNGG 2 cut(s) 444, 607
BsmAI GTCTC 1 cut(s) 327
BsmFI GGGAC 3 cut(s) 5, 401, 516
BsnI GGCC 2 cut(s) 76, 882
Bso31I GGTCTC 1 cut(s) 327
Bsp143I GATC 1 cut(s) 852
Bsp19I CCATGG 1 cut(s) 241
BspANI GGCC 2 cut(s) 76, 882
BspCNI CTCAG 3 cut(s) 70, 133, 358
BspHI TCATGA 1 cut(s) 855
BspLI GGNNCC 2 cut(s) 170, 423
BspOI GCTAGC 1 cut(s) 665
BspQI GCTCTTC 1 cut(s) 840
BspTI CTTAAG 1 cut(s) 514
BspTNI GGTCTC 1 cut(s) 327
BsrDI GCAATG 2 cut(s) 208, 739
BsrI ACTGG 1 cut(s) 395
BssECI CCNNGG 5 cut(s) 172, 241, 606, 619, 840
BssMI GATC 1 cut(s) 852
BssT1I CCWWGG 4 cut(s) 172, 241, 619, 840
Bst2UI CCWGG 1 cut(s) 166
Bst4CI ACNGT 1 cut(s) 658
Bst6I CTCTTC 4 cut(s) 45, 311, 689, 840
BstAFI CTTAAG 1 cut(s) 514
BstAPI GCANNNNNTGC 1 cut(s) 207
BstC8I GCNNGC 6 cut(s) 179, 208, 486, 527, 663, 816
BstDEI CTNAG 3 cut(s) 78, 120, 366
BstDSI CCRYGG 1 cut(s) 241
BstF5I GGATG 1 cut(s) 646
BstH2I RGCGCY 1 cut(s) 651
BstHHI GCGC 1 cut(s) 650
BstKTI GATC 1 cut(s) 855
BstMAI GTCTC 1 cut(s) 327
BstMBI GATC 1 cut(s) 852
BstMWI GCNNNNNNNGC 1 cut(s) 207
BstNI CCWGG 1 cut(s) 166
BstNSI RCATGY 3 cut(s) 108, 210, 456
BstSCI CCNGG 2 cut(s) 164, 606
BstV2I GAAGAC 1 cut(s) 12
BstXI CCANNNNNNTGG 2 cut(s) 641, 890
BsuI GTATCC 1 cut(s) 93
BsuRI GGCC 2 cut(s) 76, 882
BtgI CCRYGG 1 cut(s) 241
BtsCI GGATG 1 cut(s) 646
BtsIMutI CAGTG 1 cut(s) 363
Cac8I GCNNGC 6 cut(s) 179, 208, 486, 527, 663, 816
CciI TCATGA 1 cut(s) 855
CfoI GCGC 1 cut(s) 650
Cfr13I GGNCC 1 cut(s) 421
Csp6I GTAC 3 cut(s) 355, 750, 802
CspCI CAANNNNNGTGG 2 cut(s) 625, 660
CviAII CATG 8 cut(s) 60, 105, 207, 242, 439, 453, 671, 856
CviQI GTAC 3 cut(s) 355, 750, 802
DdeI CTNAG 3 cut(s) 78, 120, 366
DpnI GATC 1 cut(s) 854
DpnII GATC 1 cut(s) 852
DraIII CACNNNGTG 1 cut(s) 763
Eam1104I CTCTTC 4 cut(s) 45, 311, 689, 840
EarI CTCTTC 4 cut(s) 45, 311, 689, 840
Eco130I CCWWGG 4 cut(s) 172, 241, 619, 840
Eco31I GGTCTC 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 421
Eco57I CTGAAG 2 cut(s) 12, 164
EcoO109I RGGNCCY 1 cut(s) 421
EcoRI GAATTC 1 cut(s) 772
EcoRII CCWGG 1 cut(s) 164
EcoT14I CCWWGG 4 cut(s) 172, 241, 619, 840
EcoT22I ATGCAT 1 cut(s) 208
ErhI CCWWGG 4 cut(s) 172, 241, 619, 840
FaeI CATG 8 cut(s) 63, 108, 210, 245, 442, 456, 674, 859
FaqI GGGAC 3 cut(s) 5, 401, 516
FatI CATG 8 cut(s) 59, 104, 206, 241, 438, 452, 670, 855
FbaI TGATCA 1 cut(s) 852
FokI GGATG 1 cut(s) 653
FspBI CTAG 5 cut(s) 17, 173, 444, 468, 662
GlaI GCGC 1 cut(s) 649
GsaI CCCAGC 2 cut(s) 26, 894
GsuI CTGGAG 1 cut(s) 72
HaeII RGCGCY 1 cut(s) 651
HaeIII GGCC 2 cut(s) 76, 882
HapII CCGG 1 cut(s) 608
HhaI GCGC 1 cut(s) 650
Hin1II CATG 8 cut(s) 63, 108, 210, 245, 442, 456, 674, 859
Hin6I GCGC 1 cut(s) 648
HinP1I GCGC 1 cut(s) 648
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 2 cut(s) 579, 906
HpaII CCGG 1 cut(s) 608
HphI GGTGA 2 cut(s) 775, 781
Hpy166II GTNNAC 1 cut(s) 902
Hpy188I TCNGA 4 cut(s) 144, 256, 578, 778
Hpy188III TCNNGA 5 cut(s) 89, 122, 386, 458, 856
Hpy8I GTNNAC 1 cut(s) 902
HpyAV CCTTC 2 cut(s) 612, 775
HpyCH4III ACNGT 1 cut(s) 658
HpyCH4V TGCA 3 cut(s) 206, 210, 871
HpyF10VI GCNNNNNNNGC 1 cut(s) 207
HpyF3I CTNAG 3 cut(s) 78, 120, 366
Hsp92II CATG 8 cut(s) 63, 108, 210, 245, 442, 456, 674, 859
HspAI GCGC 1 cut(s) 648
Ksp22I TGATCA 1 cut(s) 852
Kzo9I GATC 1 cut(s) 852
LguI GCTCTTC 1 cut(s) 840
LmnI GCTCC 3 cut(s) 168, 267, 729
MaeI CTAG 5 cut(s) 17, 173, 444, 468, 662
MalI GATC 1 cut(s) 854
MboI GATC 1 cut(s) 852
MboII GAAGA 8 cut(s) 17, 62, 122, 217, 328, 334, 706, 857
MluCI AATT 2 cut(s) 92, 772
MlyI GAGTC 2 cut(s) 573, 900
MmeI TCCRAC 1 cut(s) 658
Mph1103I ATGCAT 1 cut(s) 208
MseI TTAA 3 cut(s) 95, 431, 515
MslI CAYNNNNRTG 1 cut(s) 639
MspA1I CMGCKG 2 cut(s) 32, 890
MspCI CTTAAG 1 cut(s) 514
MspI CCGG 1 cut(s) 608
MspR9I CCNGG 2 cut(s) 166, 608
MvaI CCWGG 1 cut(s) 166
MwoI GCNNNNNNNGC 1 cut(s) 207
NciI CCSGG 1 cut(s) 608
NcoI CCATGG 1 cut(s) 241
NdeII GATC 1 cut(s) 852
NheI GCTAGC 1 cut(s) 661
NlaIII CATG 8 cut(s) 63, 108, 210, 245, 442, 456, 674, 859
NlaIV GGNNCC 2 cut(s) 170, 423
NsiI ATGCAT 1 cut(s) 208
NspI RCATGY 3 cut(s) 108, 210, 456
PaeI GCATGC 1 cut(s) 210
PagI TCATGA 1 cut(s) 855
PciSI GCTCTTC 1 cut(s) 840
PleI GAGTC 2 cut(s) 573, 900
PpsI GAGTC 2 cut(s) 573, 900
PpuMI RGGWCCY 1 cut(s) 421
Psp5II RGGWCCY 1 cut(s) 421
Psp6I CCWGG 1 cut(s) 164
PspFI CCCAGC 2 cut(s) 22, 890
PspGI CCWGG 1 cut(s) 164
PspN4I GGNNCC 2 cut(s) 170, 423
PspPI GGNCC 1 cut(s) 421
PspPPI RGGWCCY 1 cut(s) 421
PvuII CAGCTG 2 cut(s) 32, 890
RsaI GTAC 3 cut(s) 356, 751, 803
RsaNI GTAC 3 cut(s) 355, 750, 802
RseI CAYNNNNRTG 1 cut(s) 639
SapI GCTCTTC 1 cut(s) 840
SaqAI TTAA 3 cut(s) 95, 431, 515
Sau3AI GATC 1 cut(s) 852
Sau96I GGNCC 1 cut(s) 421
SchI GAGTC 2 cut(s) 573, 900
ScrFI CCNGG 2 cut(s) 166, 608
SinI GGWCC 1 cut(s) 421
SmiMI CAYNNNNRTG 1 cut(s) 639
SmlI CTYRAG 2 cut(s) 514, 809
SmoI CTYRAG 2 cut(s) 514, 809
SphI GCATGC 1 cut(s) 210
Sse9I AATT 2 cut(s) 92, 772
SspI AATATT 1 cut(s) 382
SspMI CTAG 5 cut(s) 17, 173, 444, 468, 662
StyD4I CCNGG 2 cut(s) 164, 606
StyI CCWWGG 4 cut(s) 172, 241, 619, 840
TaaI ACNGT 1 cut(s) 658
TasI AATT 2 cut(s) 92, 772
TatI WGTACW 1 cut(s) 749
Tru1I TTAA 3 cut(s) 95, 431, 515
Tru9I TTAA 3 cut(s) 95, 431, 515
TscAI CASTG 1 cut(s) 370
TspDTI ATGAA 1 cut(s) 872
TspRI CASTG 1 cut(s) 370
Vha464I CTTAAG 1 cut(s) 514
VpaK11BI GGWCC 1 cut(s) 421
XapI RAATTY 1 cut(s) 772
XceI RCATGY 3 cut(s) 108, 210, 456
XcmI CCANNNNNNNNNTGG 1 cut(s) 641
XmaJI CCTAGG 1 cut(s) 172
XspI CTAG 5 cut(s) 17, 173, 444, 468, 662
Zsp2I ATGCAT 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.