Rw0G014290

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00641
OG0000590
Physical Location & Seq
Forward (+)
6601 .. 8151
1551 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G014290.1

Sequence Viewer

Length: 996 bp
ATGGGAGAAGTTGATCCAGCTTTCATCCAAGACCCTGAACTAGACCTAAAACTCTCCATCATCGAACCTGAAGGCATACCATTGATAGACCTCTCTCCATTAACGCTCCCAGACTCCATTTCTGACCCTAAAGCCATTAAAGTGCTTCGCAAAAAGATTGATACTGCGGCCAGGAAGTTCTTTGCTCAGCCTTTGGAGGAGAAGAGAAAGATTAGGAGGGATGAAAAATGTGTGGTAGGTTACTATGACACTGAGCATAACAAAAATGTTAGAGACTGGAAGGAGGTGTTTGATTTCCTTGTGGAGGAGCCTACATTAGTACCCTCCTCGACTGAGCCTGATGACAAGGAGGAGACACAGTGGTTTAATCAATGGCCTGAGAACCCCCCTGAATTAAGGGAGGTGCTTGAAGAATATTCTCAAGAAGTAGAAAAGCTATCTCTAAAGTTGATGGGACTAATTGCGTTGAGCCTGGGCTTGCCAGAAGACAGGTTCAAAGGCTACTTCAAGGACCAAACCAGTTTTATCAGACTCAATCACTATCCACCTTGCCCTTCCCCTCAGTTAGCACTCGGTGTTGGGCGCCACAAGGATGGTGGTGCCTTAACTGTACTGGCTCAAGATGAGGTTGGAGGATTGGAGGTGAAGCGAAAAACAGATGGAGAGTGGATTCGGGTCAGACCTACACCAAATGCCTATATCATCAATGTTGGTGACATTATTCAGGTATGGAGCAACGATAAATATGAGAGTGTGGAACATAGGGTGATGGTAAACTCAGAGAAGGAGAGGTTTTCTGTTCCCTTCTTTCTTAACCCAGCACACTACACAGAAGTGAAGCCTTTGGAGGAGCTCACCAATAAACAAAACCCTGCTAAGTATAGGCCCTATAGCTGGGGCAAGTTTCTGACTCTCAGAAAGCTCAGTAATTTCAAGAAACTCAAAGCTGAAAACATCCAAATCTATCATTTCAGGGTTTCAGATCAAGCGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

331

Amino Acids

38.3

Weight (kDa)

5.6

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 49 - 128 2e-12 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 174 - 273 3.1e-34 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19000 AT3G19000
fragaria_vesca FvH4_2g07071 FvH4_2g07080 FvH4_2g07080 FvH4_2g07081 FvH4_2g07082 FvH4_2g07082
malus_domestica MD05G1074000.v1.1 MD10G1088100.v1.1 MD10G1088200.v1.1 MD14G1207600.v1.1
prunus_persica Prupe.8G114100_v2.0.a1 Prupe.8G114200_v2.0.a1 Prupe.8G114300_v2.0.a1 Prupe.8G114500_v2.0.a1 Prupe.8G114800_v2.0.a1 Prupe.8G115100_v2.0.a1
pyrus_communis pycom05g06640 pycom05g06660 pycom05g06670 pycom10g07050
rosa_chinensis RchiOBHm_Chr6g0244141 RchiOBHm_Chr6g0259511 RchiOBHm_Chr6g0259521 RchiOBHm_Chr6g0259531 RchiOBHm_Chr6g0259551 RchiOBHm_Chr6g0259561
rosa_laevigata RLG00000014522 RLG00000014523 RLG00000014524 RLG00000014525 RLG00000014526 RLG00000014527
rosa_multiflora Rmu_ssc0000050.1_g000019 Rmu_ssc0000050.1_g000020 Rmu_ssc0000050.1_g000026 Rmu_ssc0000050.1_g000027 Rmu_ssc0000050.1_g000028
rosa_roxburghii Rroxscaffold_7G00206780 Rroxscaffold_7G00206790 Rroxscaffold_7G00206850 Rroxscaffold_7G00206860 Rroxscaffold_7G00206870 Rroxscaffold_7G00206880
rosa_rugosa Rorug05G0589100 Rorug05G0589200.1 Rorug05G0589300 Rorug05G0589400 Rorug05G0589500 Rorug05G0589600
rosa_samantha Rh4CG096000 Rh5BG231700 Rh6AG104900 Rh6AG105000 Rh6AG105100 Rh6AG105500 Rh6AG105600 Rh6BG098500 Rh6BG098700 Rh6BG098800 Rh6BG099000 Rh6BG099100 Rh6CG094200 Rh6CG094300 Rh6CG094400 Rh6CG094500 Rh6CG094600 Rh6DG088300 Rh6DG088400 Rh6DG088500 Rh6DG088600 Rh6DG088700
rosa_wichuraiana Rw0G014270 Rw0G014280 Rw0G014290 Rw6G009080 Rw6G009090 Rw6G009100 Rw6G009110 Rw6G009120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 582, 599
AciI CCGC 1 cut(s) 167
AclWI GGATC 1 cut(s) 8
AcoI YGGCCR 1 cut(s) 168
AcuI CTGAAG 1 cut(s) 90
AcyI GRCGYC 1 cut(s) 583
AdeI CACNNNGTG 1 cut(s) 575
AfaI GTAC 2 cut(s) 321, 612
AfiI CCNNNNNNNGG 2 cut(s) 304, 894
AgsI TTSAA 4 cut(s) 410, 496, 508, 934
AjnI CCWGG 2 cut(s) 170, 471
AleI CACNNNNGTG 1 cut(s) 833
AluBI AGCT 6 cut(s) 20, 436, 853, 894, 922, 947
AluI AGCT 6 cut(s) 20, 436, 853, 894, 922, 947
Alw21I GWGCWC 1 cut(s) 855
Alw26I GTCTC 2 cut(s) 267, 347
AlwI GGATC 1 cut(s) 8
AoxI GGCC 3 cut(s) 168, 374, 884
AspLEI GCGC 1 cut(s) 585
AspS9I GGNCC 2 cut(s) 511, 885
AsuHPI GGTGA 4 cut(s) 655, 725, 778, 847
AvaII GGWCC 1 cut(s) 511
BaeI ACNNNNGTAYC 2 cut(s) 303, 336
BanI GGYRCC 2 cut(s) 582, 599
BanII GRGCYC 1 cut(s) 855
BbsI GAAGAC 1 cut(s) 492
Bbv12I GWGCWC 1 cut(s) 855
BccI CCATC 5 cut(s) 65, 445, 587, 653, 763
BciT130I CCWGG 2 cut(s) 172, 473
BcoDI GTCTC 2 cut(s) 267, 347
BfaI CTAG 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 889
BfoI RGCGCY 1 cut(s) 586
BisI GCNGC 1 cut(s) 168
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 1 cut(s) 169
Bme1390I CCNGG 2 cut(s) 172, 473
Bme18I GGWCC 1 cut(s) 511
BmgT120I GGNCC 2 cut(s) 511, 885
BmiI GGNNCC 3 cut(s) 309, 584, 601
BmrFI CCNGG 2 cut(s) 172, 473
BpiI GAAGAC 1 cut(s) 492
Bpu1102I GCTNAGC 1 cut(s) 186
BpuEI CTTGAG 2 cut(s) 405, 603
BsaHI GRCGYC 1 cut(s) 583
BsaJI CCNNGG 1 cut(s) 472
BsaXI ACNNNNNCTCC 2 cut(s) 344, 374
Bsc4I CCNNNNNNNGG 2 cut(s) 304, 894
Bse1I ACTGG 3 cut(s) 281, 519, 618
BseBI CCWGG 2 cut(s) 172, 473
BseDI CCNNGG 1 cut(s) 472
BseGI GGATG 4 cut(s) 24, 226, 598, 954
BseLI CCNNNNNNNGG 2 cut(s) 304, 894
BseMII CTCAG 8 cut(s) 200, 243, 324, 369, 575, 792, 928, 937
BseNI ACTGG 3 cut(s) 281, 519, 618
BseRI GAGGAG 5 cut(s) 212, 316, 320, 365, 863
BseYI CCCAGC 2 cut(s) 817, 894
BshFI GGCC 3 cut(s) 170, 376, 886
BshNI GGYRCC 2 cut(s) 582, 599
BsiHKAI GWGCWC 1 cut(s) 855
BslFI GGGAC 1 cut(s) 468
BslI CCNNNNNNNGG 2 cut(s) 304, 894
BsmAI GTCTC 2 cut(s) 267, 347
BsmFI GGGAC 1 cut(s) 468
BsnI GGCC 3 cut(s) 170, 376, 886
Bsp1286I GDGCHC 1 cut(s) 855
Bsp143I GATC 2 cut(s) 13, 982
Bsp1720I GCTNAGC 1 cut(s) 186
BspACI CCGC 1 cut(s) 167
BspANI GGCC 3 cut(s) 170, 376, 886
BspCNI CTCAG 8 cut(s) 199, 244, 325, 370, 574, 791, 927, 936
BspLI GGNNCC 3 cut(s) 309, 584, 601
BspPI GGATC 1 cut(s) 8
BspT107I GGYRCC 2 cut(s) 582, 599
BsrI ACTGG 3 cut(s) 281, 519, 618
BssECI CCNNGG 1 cut(s) 472
BssMI GATC 2 cut(s) 13, 982
BssNI GRCGYC 1 cut(s) 583
Bst2UI CCWGG 2 cut(s) 172, 473
Bst4CI ACNGT 2 cut(s) 360, 610
Bst6I CTCTTC 1 cut(s) 197
BstACI GRCGYC 1 cut(s) 583
BstC8I GCNNGC 1 cut(s) 479
BstDEI CTNAG 9 cut(s) 186, 252, 333, 378, 561, 778, 876, 914, 923
BstENI CCTNNNNNAGG 1 cut(s) 302
BstF5I GGATG 4 cut(s) 24, 226, 598, 954
BstH2I RGCGCY 1 cut(s) 586
BstHHI GCGC 1 cut(s) 585
BstKTI GATC 2 cut(s) 16, 985
BstMAI GTCTC 2 cut(s) 267, 347
BstMBI GATC 2 cut(s) 13, 982
BstNI CCWGG 2 cut(s) 172, 473
BstSCI CCNGG 2 cut(s) 170, 471
BstSFI CTRYAG 1 cut(s) 889
BstV2I GAAGAC 1 cut(s) 492
BstXI CCANNNNNNTGG 1 cut(s) 593
BsuRI GGCC 3 cut(s) 170, 376, 886
BtsCI GGATG 4 cut(s) 24, 226, 598, 954
BtsIMutI CAGTG 2 cut(s) 249, 365
Cac8I GCNNGC 1 cut(s) 479
CfoI GCGC 1 cut(s) 585
Cfr13I GGNCC 2 cut(s) 511, 885
Csp6I GTAC 2 cut(s) 320, 611
CspCI CAANNNNNGTGG 2 cut(s) 577, 612
CviQI GTAC 2 cut(s) 320, 611
DdeI CTNAG 9 cut(s) 186, 252, 333, 378, 561, 778, 876, 914, 923
DinI GGCGCC 1 cut(s) 584
DpnI GATC 2 cut(s) 15, 984
DpnII GATC 2 cut(s) 13, 982
DraIII CACNNNGTG 1 cut(s) 575
EaeI YGGCCR 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
Ecl136II GAGCTC 1 cut(s) 853
Eco24I GRGCYC 1 cut(s) 855
Eco47I GGWCC 1 cut(s) 511
Eco53kI GAGCTC 1 cut(s) 853
Eco57I CTGAAG 1 cut(s) 90
EcoICRI GAGCTC 1 cut(s) 853
EcoNI CCTNNNNNAGG 1 cut(s) 302
EcoO109I RGGNCCY 1 cut(s) 885
EcoRII CCWGG 2 cut(s) 170, 471
EcoT38I GRGCYC 1 cut(s) 855
EgeI GGCGCC 1 cut(s) 584
EheI GGCGCC 1 cut(s) 584
FaiI YATR 9 cut(s) 77, 246, 258, 699, 730, 747, 762, 882, 891
FaqI GGGAC 1 cut(s) 468
Fnu4HI GCNGC 1 cut(s) 168
FokI GGATG 4 cut(s) 11, 233, 605, 941
FriOI GRGCYC 1 cut(s) 855
Fsp4HI GCNGC 1 cut(s) 168
FspBI CTAG 1 cut(s) 41
GlaI GCGC 1 cut(s) 584
GluI GCNGC 1 cut(s) 168
GsaI CCCAGC 2 cut(s) 821, 898
HaeII RGCGCY 1 cut(s) 586
HaeIII GGCC 3 cut(s) 170, 376, 886
HhaI GCGC 1 cut(s) 585
Hin1I GRCGYC 1 cut(s) 583
Hin6I GCGC 1 cut(s) 583
HinP1I GCGC 1 cut(s) 583
HinfI GANTC 4 cut(s) 113, 531, 670, 910
HphI GGTGA 4 cut(s) 655, 725, 778, 847
Hpy166II GTNNAC 1 cut(s) 775
Hpy188I TCNGA 7 cut(s) 124, 530, 680, 781, 909, 917, 982
Hpy188III TCNNGA 3 cut(s) 422, 620, 934
Hpy8I GTNNAC 1 cut(s) 775
HpyAV CCTTC 5 cut(s) 65, 274, 564, 778, 814
HpyCH4III ACNGT 2 cut(s) 360, 610
HpyF3I CTNAG 9 cut(s) 186, 252, 333, 378, 561, 778, 876, 914, 923
Hsp92I GRCGYC 1 cut(s) 583
HspAI GCGC 1 cut(s) 583
KasI GGCGCC 1 cut(s) 582
Kzo9I GATC 2 cut(s) 13, 982
LmnI GCTCC 4 cut(s) 111, 307, 732, 850
MaeI CTAG 1 cut(s) 41
MaeIII GTNAC 2 cut(s) 239, 713
MalI GATC 2 cut(s) 15, 984
MboI GATC 2 cut(s) 13, 982
MboII GAAGA 3 cut(s) 214, 422, 497
MhlI GDGCHC 1 cut(s) 855
MluCI AATT 3 cut(s) 392, 459, 928
Mly113I GGCGCC 1 cut(s) 583
MlyI GAGTC 3 cut(s) 107, 525, 904
MmeI TCCRAC 1 cut(s) 610
MseI TTAA 6 cut(s) 101, 138, 366, 395, 605, 813
MslI CAYNNNNRTG 3 cut(s) 140, 591, 833
MspR9I CCNGG 2 cut(s) 172, 473
MvaI CCWGG 2 cut(s) 172, 473
NarI GGCGCC 1 cut(s) 583
NdeII GATC 2 cut(s) 13, 982
NlaIV GGNNCC 3 cut(s) 309, 584, 601
NmuCI GTSAC 1 cut(s) 713
OliI CACNNNNGTG 1 cut(s) 833
PfeI GAWTC 1 cut(s) 670
PkrI GCNGC 1 cut(s) 169
PleI GAGTC 3 cut(s) 107, 525, 904
PluTI GGCGCC 1 cut(s) 586
PpsI GAGTC 3 cut(s) 107, 525, 904
Psp124BI GAGCTC 1 cut(s) 855
Psp6I CCWGG 2 cut(s) 170, 471
PspFI CCCAGC 2 cut(s) 817, 894
PspGI CCWGG 2 cut(s) 170, 471
PspN4I GGNNCC 3 cut(s) 309, 584, 601
PspPI GGNCC 2 cut(s) 511, 885
RsaI GTAC 2 cut(s) 321, 612
RsaNI GTAC 2 cut(s) 320, 611
RseI CAYNNNNRTG 3 cut(s) 140, 591, 833
SacI GAGCTC 1 cut(s) 855
SaqAI TTAA 6 cut(s) 101, 138, 366, 395, 605, 813
SatI GCNGC 1 cut(s) 168
Sau3AI GATC 2 cut(s) 13, 982
Sau96I GGNCC 2 cut(s) 511, 885
SchI GAGTC 3 cut(s) 107, 525, 904
ScrFI CCNGG 2 cut(s) 172, 473
SduI GDGCHC 1 cut(s) 855
SfcI CTRYAG 1 cut(s) 889
SfoI GGCGCC 1 cut(s) 584
SinI GGWCC 1 cut(s) 511
SmiMI CAYNNNNRTG 3 cut(s) 140, 591, 833
SmlI CTYRAG 2 cut(s) 420, 618
SmoI CTYRAG 2 cut(s) 420, 618
Sse9I AATT 3 cut(s) 392, 459, 928
SsiI CCGC 1 cut(s) 167
SspDI GGCGCC 1 cut(s) 582
SspI AATATT 1 cut(s) 416
SspMI CTAG 1 cut(s) 41
SstI GAGCTC 1 cut(s) 855
StyD4I CCNGG 2 cut(s) 170, 471
TaaI ACNGT 2 cut(s) 360, 610
TaqI TCGA 2 cut(s) 63, 329
TasI AATT 3 cut(s) 392, 459, 928
TatI WGTACW 1 cut(s) 610
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 1 cut(s) 670
Tru1I TTAA 6 cut(s) 101, 138, 366, 395, 605, 813
Tru9I TTAA 6 cut(s) 101, 138, 366, 395, 605, 813
TscAI CASTG 2 cut(s) 256, 365
TseFI GTSAC 1 cut(s) 713
Tsp45I GTSAC 1 cut(s) 713
TspDTI ATGAA 2 cut(s) 13, 237
TspRI CASTG 2 cut(s) 256, 365
VpaK11BI GGWCC 1 cut(s) 511
XagI CCTNNNNNAGG 1 cut(s) 302
XcmI CCANNNNNNNNNTGG 1 cut(s) 593
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.