MD14G1207600.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000590
Physical Location & Seq
Forward (+)
29464497 .. 29465903
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1207600.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGGGAGAGGTTGATCCAGCTTTCATCCAAGACCCCGAGCACAGGCCAAAACTCTCCGCCACCGAAGTCGAAGGCATACCGTTGATCGACCTCTCTCTGATAAACTCTCCACACTCCGTTACCGACCCCAGAGTCTTTGAAGGGCTTGTTGCAGAAATAGGCAATGCATGCAAGGACTGGGGTTTCTTCCAAGTGATCAACCACGGGGTTCTGTTGGAGAAGCGCCAAAGGATCGAAGCTGCTGCTCGGAAGTTCTTTGCGCTGCCTTTGGAGGAGAAGAGGAAGATTAGAAGGGATGAAGGGAATGTGTTGGGTTATTATGATACCGAGCATACCAAGAATGTCAGGGACTGGAAGGAGGTGTTTGATTTCACTGTGGAAGAGCCTACGTTAGTCCCGGCTTCGCCTGACCCGGAGGACACGGAAGAGACGGAGTGGTATAACCAATGGCCGGAGTATCCTCCTGAACTAAGGAATGCTTGTGAAGAATATGCTCGAGAAGTCGAAAATCTAGCTCTAAAGTTGATGGGATTAATTGCCTTGGGCCTAGGCTTGCCGAAAGACAGGTTCAACAGCTACTTCAAAAACCAAACCAGTTTTCTCAGACTCAACCACTATCCGCCTTGCCCTTCACCTCAGTTAGCTCTCGGCGTTGGCCGGCACAAGGATGGCGGTGCTTTGACAGTGCTGGCTCAGGATGATGTTGGAGGGCTGGAAGTGAAGAGAAAAACAGACGGAGTGTGGATACGGGTTAAACCTACCCCAAACGCCTTTATCATCAATGTCGGTGACGTTATTCAGGTTTGGAGCAACGAGAGATACGAAAGCGTGGAGCACAGGGCGATGGTGAATTCGGAGAAGGAAAGGTTTTCCATTCCCTACTTCCTCAACCCAGCTCACTACACACAAGTCAAGCCATTGGAGGAGCTGATCAATGAACAAAACCCTGCTAAGTACAAGCCCTACTGCTGGGGAAAGTTTATTACTCACAGAAAGCTCAGTAACTTCAAGAAGCTTAACACCGAAAACATCCAGATTTCTCATTTCAAAATATCGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

354

Amino Acids

40.62

Weight (kDa)

5.69

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 26 - 152 9.8e-32 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 199 - 298 3.7e-34 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19000 AT3G19000
fragaria_vesca FvH4_2g07071 FvH4_2g07080 FvH4_2g07080 FvH4_2g07081 FvH4_2g07082 FvH4_2g07082
malus_domestica MD05G1074000.v1.1 MD10G1088100.v1.1 MD10G1088200.v1.1 MD14G1207600.v1.1
prunus_persica Prupe.8G114100_v2.0.a1 Prupe.8G114200_v2.0.a1 Prupe.8G114300_v2.0.a1 Prupe.8G114500_v2.0.a1 Prupe.8G114800_v2.0.a1 Prupe.8G115100_v2.0.a1
pyrus_communis pycom05g06640 pycom05g06660 pycom05g06670 pycom10g07050
rosa_chinensis RchiOBHm_Chr6g0244141 RchiOBHm_Chr6g0259511 RchiOBHm_Chr6g0259521 RchiOBHm_Chr6g0259531 RchiOBHm_Chr6g0259551 RchiOBHm_Chr6g0259561
rosa_laevigata RLG00000014522 RLG00000014523 RLG00000014524 RLG00000014525 RLG00000014526 RLG00000014527
rosa_multiflora Rmu_ssc0000050.1_g000019 Rmu_ssc0000050.1_g000020 Rmu_ssc0000050.1_g000026 Rmu_ssc0000050.1_g000027 Rmu_ssc0000050.1_g000028
rosa_roxburghii Rroxscaffold_7G00206780 Rroxscaffold_7G00206790 Rroxscaffold_7G00206850 Rroxscaffold_7G00206860 Rroxscaffold_7G00206870 Rroxscaffold_7G00206880
rosa_rugosa Rorug05G0589100 Rorug05G0589200.1 Rorug05G0589300 Rorug05G0589400 Rorug05G0589500 Rorug05G0589600
rosa_samantha Rh4CG096000 Rh5BG231700 Rh6AG104900 Rh6AG105000 Rh6AG105100 Rh6AG105500 Rh6AG105600 Rh6BG098500 Rh6BG098700 Rh6BG098800 Rh6BG099000 Rh6BG099100 Rh6CG094200 Rh6CG094300 Rh6CG094400 Rh6CG094500 Rh6CG094600 Rh6DG088300 Rh6DG088400 Rh6DG088500 Rh6DG088600 Rh6DG088700
rosa_wichuraiana Rw0G014270 Rw0G014280 Rw0G014290 Rw6G009080 Rw6G009090 Rw6G009100 Rw6G009110 Rw6G009120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 131
AciI CCGC 3 cut(s) 57, 620, 672
AclWI GGATC 2 cut(s) 8, 239
AcoI YGGCCR 2 cut(s) 449, 655
AcsI RAATTY 1 cut(s) 850
AfaI GTAC 1 cut(s) 956
AfiI CCNNNNNNNGG 4 cut(s) 42, 451, 664, 969
AgsI TTSAA 5 cut(s) 140, 571, 583, 1009, 1048
AjuI GAANNNNNNNTTGG 2 cut(s) 582, 614
AluBI AGCT 9 cut(s) 20, 239, 515, 576, 644, 896, 928, 997, 1015
AluI AGCT 9 cut(s) 20, 239, 515, 576, 644, 896, 928, 997, 1015
Alw21I GWGCWC 2 cut(s) 42, 837
Alw26I GTCTC 1 cut(s) 422
AlwI GGATC 2 cut(s) 8, 239
AlwNI CAGNNNCTG 1 cut(s) 351
Ama87I CYCGRG 2 cut(s) 35, 495
AoxI GGCC 4 cut(s) 44, 449, 544, 655
ApeKI GCWGC 3 cut(s) 239, 242, 262
ApoI RAATTY 1 cut(s) 850
AseI ATTAAT 1 cut(s) 533
AspA2I CCTAGG 1 cut(s) 547
AspLEI GCGC 2 cut(s) 225, 262
AspS9I GGNCC 1 cut(s) 544
AsuC2I CCSGG 2 cut(s) 398, 413
AsuHPI GGTGA 3 cut(s) 624, 800, 859
AvaI CYCGRG 2 cut(s) 35, 495
AvrII CCTAGG 1 cut(s) 547
Bbv12I GWGCWC 2 cut(s) 42, 837
BbvI GCAGC 3 cut(s) 226, 229, 249
BccI CCATC 3 cut(s) 520, 662, 838
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BciVI GTATCC 2 cut(s) 468, 738
BclI TGATCA 2 cut(s) 195, 930
BcnI CCSGG 2 cut(s) 398, 413
BcoDI GTCTC 1 cut(s) 422
BfaI CTAG 2 cut(s) 512, 548
BfoI RGCGCY 1 cut(s) 226
BfuI GTATCC 2 cut(s) 468, 738
BisI GCNGC 3 cut(s) 240, 243, 263
BlnI CCTAGG 1 cut(s) 547
BlsI GCNGC 3 cut(s) 241, 244, 264
Bme1390I CCNGG 2 cut(s) 398, 413
BmeT110I CYCGRG 2 cut(s) 35, 495
BmgT120I GGNCC 1 cut(s) 544
BmrFI CCNGG 2 cut(s) 398, 413
BmrI ACTGGG 1 cut(s) 187
BmuI ACTGGG 1 cut(s) 187
Bpu10I CCTNAGC 1 cut(s) 693
BpuMI CCSGG 2 cut(s) 398, 413
BsaJI CCNNGG 3 cut(s) 202, 540, 547
Bsc4I CCNNNNNNNGG 4 cut(s) 42, 451, 664, 969
Bse118I RCCGGY 1 cut(s) 657
Bse1I ACTGG 3 cut(s) 182, 356, 594
Bse3DI GCAATG 1 cut(s) 169
BseDI CCNNGG 3 cut(s) 202, 540, 547
BseGI GGATG 5 cut(s) 24, 301, 673, 703, 1029
BseLI CCNNNNNNNGG 4 cut(s) 42, 451, 664, 969
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 4 cut(s) 616, 650, 707, 1012
BseNI ACTGG 3 cut(s) 182, 356, 594
BseRI GAGGAG 2 cut(s) 287, 938
BseXI GCAGC 3 cut(s) 226, 229, 249
BseYI CCCAGC 2 cut(s) 892, 969
BshFI GGCC 4 cut(s) 46, 451, 546, 657
BsiHKAI GWGCWC 2 cut(s) 42, 837
BsiHKCI CYCGRG 2 cut(s) 35, 495
BsiSI CCGG 4 cut(s) 398, 413, 452, 658
BslFI GGGAC 2 cut(s) 362, 380
BslI CCNNNNNNNGG 4 cut(s) 42, 451, 664, 969
BsmAI GTCTC 1 cut(s) 422
BsmBI CGTCTC 1 cut(s) 422
BsmFI GGGAC 2 cut(s) 362, 380
BsmI GAATGC 1 cut(s) 481
BsnI GGCC 4 cut(s) 46, 451, 546, 657
BsoBI CYCGRG 2 cut(s) 35, 495
Bsp1286I GDGCHC 2 cut(s) 42, 837
Bsp143I GATC 5 cut(s) 13, 84, 195, 231, 930
BspACI CCGC 3 cut(s) 57, 620, 672
BspANI GGCC 4 cut(s) 46, 451, 546, 657
BspCNI CTCAG 4 cut(s) 615, 649, 706, 1011
BspPI GGATC 2 cut(s) 8, 239
BspQI GCTCTTC 1 cut(s) 375
BsrDI GCAATG 1 cut(s) 169
BsrFI RCCGGY 1 cut(s) 657
BsrI ACTGG 3 cut(s) 182, 356, 594
BssAI RCCGGY 1 cut(s) 657
BssECI CCNNGG 3 cut(s) 202, 540, 547
BssMI GATC 5 cut(s) 13, 84, 195, 231, 930
BssT1I CCWWGG 2 cut(s) 540, 547
Bst4CI ACNGT 3 cut(s) 81, 376, 685
Bst6I CTCTTC 4 cut(s) 272, 375, 420, 716
BstAPI GCANNNNNTGC 1 cut(s) 168
BstC8I GCNNGC 4 cut(s) 169, 554, 659, 690
BstDEI CTNAG 6 cut(s) 470, 602, 636, 693, 951, 998
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 5 cut(s) 24, 301, 673, 703, 1029
BstH2I RGCGCY 1 cut(s) 226
BstHHI GCGC 2 cut(s) 225, 262
BstKTI GATC 5 cut(s) 16, 87, 198, 234, 933
BstMAI GTCTC 1 cut(s) 422
BstMBI GATC 5 cut(s) 13, 84, 195, 231, 930
BstMWI GCNNNNNNNGC 1 cut(s) 168
BstNSI RCATGY 1 cut(s) 171
BstSCI CCNGG 2 cut(s) 396, 411
BstV1I GCAGC 3 cut(s) 226, 229, 249
BsuI GTATCC 2 cut(s) 468, 738
BsuRI GGCC 4 cut(s) 46, 451, 546, 657
BtgI CCRYGG 1 cut(s) 202
BtgZI GCGATG 1 cut(s) 857
BtsCI GGATG 5 cut(s) 24, 301, 673, 703, 1029
BtsIMutI CAGTG 2 cut(s) 372, 690
Cac8I GCNNGC 4 cut(s) 169, 554, 659, 690
CaiI CAGNNNCTG 1 cut(s) 351
CfoI GCGC 2 cut(s) 225, 262
Cfr10I RCCGGY 1 cut(s) 657
Cfr13I GGNCC 1 cut(s) 544
Csp6I GTAC 1 cut(s) 955
CviAII CATG 1 cut(s) 168
CviQI GTAC 1 cut(s) 955
DdeI CTNAG 6 cut(s) 470, 602, 636, 693, 951, 998
DpnI GATC 5 cut(s) 15, 86, 197, 233, 932
DpnII GATC 5 cut(s) 13, 84, 195, 231, 930
DrdI GACNNNNNNGTC 1 cut(s) 131
DseDI GACNNNNNNGTC 1 cut(s) 131
EaeI YGGCCR 2 cut(s) 449, 655
Eam1104I CTCTTC 4 cut(s) 272, 375, 420, 716
EarI CTCTTC 4 cut(s) 272, 375, 420, 716
EciI GGCGGA 2 cut(s) 46, 609
Eco130I CCWWGG 2 cut(s) 540, 547
Eco88I CYCGRG 2 cut(s) 35, 495
EcoRI GAATTC 1 cut(s) 850
EcoT14I CCWWGG 2 cut(s) 540, 547
EcoT22I ATGCAT 1 cut(s) 169
ErhI CCWWGG 2 cut(s) 540, 547
Esp3I CGTCTC 1 cut(s) 422
FaeI CATG 1 cut(s) 171
FaiI YATR 6 cut(s) 77, 169, 321, 333, 441, 492
FalI AAGNNNNNCTT 2 cut(s) 463, 495
FaqI GGGAC 2 cut(s) 362, 380
FatI CATG 1 cut(s) 167
FbaI TGATCA 2 cut(s) 195, 930
Fnu4HI GCNGC 3 cut(s) 240, 243, 263
FokI GGATG 5 cut(s) 11, 308, 680, 710, 1016
Fsp4HI GCNGC 3 cut(s) 240, 243, 263
FspBI CTAG 2 cut(s) 512, 548
GlaI GCGC 2 cut(s) 224, 261
GluI GCNGC 3 cut(s) 240, 243, 263
GsaI CCCAGC 2 cut(s) 896, 973
HaeII RGCGCY 1 cut(s) 226
HaeIII GGCC 4 cut(s) 46, 451, 546, 657
HapII CCGG 4 cut(s) 398, 413, 452, 658
HhaI GCGC 2 cut(s) 225, 262
Hin1II CATG 1 cut(s) 171
Hin6I GCGC 2 cut(s) 223, 260
HinP1I GCGC 2 cut(s) 223, 260
HindIII AAGCTT 1 cut(s) 1013
HinfI GANTC 2 cut(s) 132, 606
HpaII CCGG 4 cut(s) 398, 413, 452, 658
HphI GGTGA 3 cut(s) 624, 800, 859
Hpy188I TCNGA 5 cut(s) 99, 249, 605, 856, 1057
Hpy188III TCNNGA 5 cut(s) 464, 497, 695, 1009, 1033
HpyAV CCTTC 7 cut(s) 65, 134, 285, 293, 349, 639, 853
HpyCH4III ACNGT 3 cut(s) 81, 376, 685
HpyCH4IV ACGT 2 cut(s) 389, 792
HpyCH4V TGCA 3 cut(s) 152, 167, 171
HpyF10VI GCNNNNNNNGC 1 cut(s) 168
HpyF3I CTNAG 6 cut(s) 470, 602, 636, 693, 951, 998
HpySE526I ACGT 2 cut(s) 389, 792
Hsp92II CATG 1 cut(s) 171
HspAI GCGC 2 cut(s) 223, 260
KroI GCCGGC 1 cut(s) 657
KroNI GCCGGC 1 cut(s) 659
Ksp22I TGATCA 2 cut(s) 195, 930
Kzo9I GATC 5 cut(s) 13, 84, 195, 231, 930
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 3 cut(s) 807, 832, 925
Lsp1109I GCAGC 3 cut(s) 226, 229, 249
MaeI CTAG 2 cut(s) 512, 548
MaeII ACGT 2 cut(s) 389, 792
MaeIII GTNAC 3 cut(s) 118, 788, 1001
MalI GATC 5 cut(s) 15, 86, 197, 233, 932
MboI GATC 5 cut(s) 13, 84, 195, 231, 930
MboII GAAGA 7 cut(s) 178, 289, 295, 392, 437, 497, 733
MhlI GDGCHC 2 cut(s) 42, 837
MluCI AATT 2 cut(s) 534, 850
MlyI GAGTC 2 cut(s) 141, 600
MmeI TCCRAC 2 cut(s) 195, 685
Mph1103I ATGCAT 1 cut(s) 169
MroNI GCCGGC 1 cut(s) 657
MseI TTAA 3 cut(s) 533, 753, 1017
MslI CAYNNNNRTG 1 cut(s) 666
MspI CCGG 4 cut(s) 398, 413, 452, 658
MspR9I CCNGG 2 cut(s) 398, 413
Mva1269I GAATGC 1 cut(s) 481
MwoI GCNNNNNNNGC 1 cut(s) 168
NaeI GCCGGC 1 cut(s) 659
NciI CCSGG 2 cut(s) 398, 413
NdeII GATC 5 cut(s) 13, 84, 195, 231, 930
NgoMIV GCCGGC 1 cut(s) 657
NlaIII CATG 1 cut(s) 171
NmeAIII GCCGAG 1 cut(s) 627
NmuCI GTSAC 1 cut(s) 788
NsiI ATGCAT 1 cut(s) 169
NspI RCATGY 1 cut(s) 171
PaeI GCATGC 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 495
PciSI GCTCTTC 1 cut(s) 375
PcsI WCGNNNNNNNCGW 1 cut(s) 819
PctI GAATGC 1 cut(s) 481
PdiI GCCGGC 1 cut(s) 659
PkrI GCNGC 3 cut(s) 241, 244, 264
PleI GAGTC 2 cut(s) 140, 600
PpsI GAGTC 2 cut(s) 140, 600
PshBI ATTAAT 1 cut(s) 533
PspFI CCCAGC 2 cut(s) 892, 969
PspPI GGNCC 1 cut(s) 544
PstNI CAGNNNCTG 1 cut(s) 351
RsaI GTAC 1 cut(s) 956
RsaNI GTAC 1 cut(s) 955
RseI CAYNNNNRTG 1 cut(s) 666
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 3 cut(s) 533, 753, 1017
SatI GCNGC 3 cut(s) 240, 243, 263
Sau3AI GATC 5 cut(s) 13, 84, 195, 231, 930
Sau96I GGNCC 1 cut(s) 544
SchI GAGTC 2 cut(s) 141, 600
ScrFI CCNGG 2 cut(s) 398, 413
SduI GDGCHC 2 cut(s) 42, 837
Sfr274I CTCGAG 1 cut(s) 495
SlaI CTCGAG 1 cut(s) 495
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 1 cut(s) 495
SmoI CTYRAG 1 cut(s) 495
SphI GCATGC 1 cut(s) 171
Sse9I AATT 2 cut(s) 534, 850
SsiI CCGC 3 cut(s) 57, 620, 672
SspMI CTAG 2 cut(s) 512, 548
StyD4I CCNGG 2 cut(s) 396, 411
StyI CCWWGG 2 cut(s) 540, 547
TaaI ACNGT 3 cut(s) 81, 376, 685
TaiI ACGT 2 cut(s) 392, 795
TaqI TCGA 5 cut(s) 69, 87, 234, 496, 504
TasI AATT 2 cut(s) 534, 850
TatI WGTACW 1 cut(s) 954
Tru1I TTAA 3 cut(s) 533, 753, 1017
Tru9I TTAA 3 cut(s) 533, 753, 1017
TscAI CASTG 2 cut(s) 379, 690
TseFI GTSAC 1 cut(s) 788
TseI GCWGC 3 cut(s) 239, 242, 262
Tsp45I GTSAC 1 cut(s) 788
TspDTI ATGAA 3 cut(s) 13, 312, 951
TspGWI ACGGA 4 cut(s) 106, 437, 446, 750
TspRI CASTG 2 cut(s) 379, 690
VspI ATTAAT 1 cut(s) 533
XapI RAATTY 1 cut(s) 850
XceI RCATGY 1 cut(s) 171
XhoI CTCGAG 1 cut(s) 495
XmaJI CCTAGG 1 cut(s) 547
XspI CTAG 2 cut(s) 512, 548
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.