Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 642 |
| AccI | GTMKAC | 2 cut(s) | 293, 452 |
| AccII | CGCG | 1 cut(s) | 32 |
| AciI | CCGC | 2 cut(s) | 30, 183 |
| AcoI | YGGCCR | 1 cut(s) | 387 |
| AdeI | CACNNNGTG | 3 cut(s) | 140, 327, 487 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 396, 642 |
| AgsI | TTSAA | 5 cut(s) | 53, 85, 175, 362, 383 |
| AjiI | CACGTC | 1 cut(s) | 427 |
| AjnI | CCWGG | 1 cut(s) | 334 |
| AluBI | AGCT | 1 cut(s) | 648 |
| AluI | AGCT | 1 cut(s) | 648 |
| Alw26I | GTCTC | 2 cut(s) | 197, 484 |
| Ama87I | CYCGRG | 1 cut(s) | 395 |
| AoxI | GGCC | 2 cut(s) | 110, 387 |
| AspS9I | GGNCC | 3 cut(s) | 43, 111, 256 |
| AsuC2I | CCSGG | 3 cut(s) | 310, 396, 397 |
| AvaI | CYCGRG | 1 cut(s) | 395 |
| AvaII | GGWCC | 2 cut(s) | 43, 256 |
| BauI | CACGAG | 1 cut(s) | 233 |
| BccI | CCATC | 1 cut(s) | 164 |
| BceAI | ACGGC | 1 cut(s) | 174 |
| BciT130I | CCWGG | 1 cut(s) | 336 |
| BclI | TGATCA | 1 cut(s) | 553 |
| BcnI | CCSGG | 3 cut(s) | 310, 396, 397 |
| BcoDI | GTCTC | 2 cut(s) | 197, 484 |
| Bme1390I | CCNGG | 4 cut(s) | 310, 336, 396, 397 |
| Bme18I | GGWCC | 2 cut(s) | 43, 256 |
| BmeT110I | CYCGRG | 1 cut(s) | 395 |
| BmgBI | CACGTC | 1 cut(s) | 427 |
| BmgT120I | GGNCC | 3 cut(s) | 43, 111, 256 |
| BmiI | GGNNCC | 4 cut(s) | 44, 45, 258, 525 |
| BmrFI | CCNGG | 4 cut(s) | 310, 336, 396, 397 |
| BmsI | GCATC | 1 cut(s) | 556 |
| BpuMI | CCSGG | 3 cut(s) | 310, 396, 397 |
| BsaBI | GATNNNNATC | 1 cut(s) | 155 |
| BsaI | GGTCTC | 1 cut(s) | 484 |
| BsaJI | CCNNGG | 5 cut(s) | 122, 334, 335, 394, 395 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 445, 475 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 396, 642 |
| Bse1I | ACTGG | 1 cut(s) | 636 |
| Bse3DI | GCAATG | 1 cut(s) | 303 |
| Bse8I | GATNNNNATC | 1 cut(s) | 155 |
| BseBI | CCWGG | 1 cut(s) | 336 |
| BseDI | CCNNGG | 5 cut(s) | 122, 334, 335, 394, 395 |
| BseGI | GGATG | 1 cut(s) | 156 |
| BseJI | GATNNNNATC | 1 cut(s) | 155 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 396, 642 |
| BseMI | GCAATG | 1 cut(s) | 303 |
| BseMII | CTCAG | 1 cut(s) | 279 |
| BseNI | ACTGG | 1 cut(s) | 636 |
| Bsh1236I | CGCG | 1 cut(s) | 32 |
| BshFI | GGCC | 2 cut(s) | 112, 389 |
| BsiHKCI | CYCGRG | 1 cut(s) | 395 |
| BsiSI | CCGG | 2 cut(s) | 309, 396 |
| BslFI | GGGAC | 3 cut(s) | 56, 242, 317 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 396, 642 |
| BsmAI | GTCTC | 2 cut(s) | 197, 484 |
| BsmBI | CGTCTC | 1 cut(s) | 197 |
| BsmFI | GGGAC | 3 cut(s) | 56, 242, 317 |
| BsmI | GAATGC | 1 cut(s) | 178 |
| BsnI | GGCC | 2 cut(s) | 112, 389 |
| Bso31I | GGTCTC | 1 cut(s) | 484 |
| BsoBI | CYCGRG | 1 cut(s) | 395 |
| Bsp143I | GATC | 2 cut(s) | 300, 553 |
| BspACI | CCGC | 2 cut(s) | 30, 183 |
| BspANI | GGCC | 2 cut(s) | 112, 389 |
| BspCNI | CTCAG | 1 cut(s) | 278 |
| BspFNI | CGCG | 1 cut(s) | 32 |
| BspHI | TCATGA | 1 cut(s) | 618 |
| BspLI | GGNNCC | 4 cut(s) | 44, 45, 258, 525 |
| BspQI | GCTCTTC | 1 cut(s) | 176 |
| BspTNI | GGTCTC | 1 cut(s) | 484 |
| BsrDI | GCAATG | 1 cut(s) | 303 |
| BsrI | ACTGG | 1 cut(s) | 636 |
| BssECI | CCNNGG | 5 cut(s) | 122, 334, 335, 394, 395 |
| BssMI | GATC | 2 cut(s) | 300, 553 |
| BssNAI | GTATAC | 1 cut(s) | 294 |
| BssSI | CACGAG | 1 cut(s) | 233 |
| BssT1I | CCWWGG | 1 cut(s) | 122 |
| Bst1107I | GTATAC | 1 cut(s) | 294 |
| Bst2BI | CACGAG | 1 cut(s) | 233 |
| Bst2UI | CCWGG | 1 cut(s) | 336 |
| Bst4CI | ACNGT | 5 cut(s) | 39, 218, 249, 433, 457 |
| Bst6I | CTCTTC | 1 cut(s) | 176 |
| BstC8I | GCNNGC | 3 cut(s) | 187, 590, 607 |
| BstDEI | CTNAG | 1 cut(s) | 265 |
| BstF5I | GGATG | 1 cut(s) | 156 |
| BstFNI | CGCG | 1 cut(s) | 32 |
| BstKTI | GATC | 2 cut(s) | 303, 556 |
| BstMAI | GTCTC | 2 cut(s) | 197, 484 |
| BstMBI | GATC | 2 cut(s) | 300, 553 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 109, 175, 594 |
| BstNI | CCWGG | 1 cut(s) | 336 |
| BstSCI | CCNGG | 4 cut(s) | 308, 334, 394, 395 |
| BstUI | CGCG | 1 cut(s) | 32 |
| BstZ17I | GTATAC | 1 cut(s) | 294 |
| BsuRI | GGCC | 2 cut(s) | 112, 389 |
| BtrI | CACGTC | 1 cut(s) | 427 |
| BtsCI | GGATG | 1 cut(s) | 156 |
| BtsI | GCAGTG | 2 cut(s) | 145, 600 |
| BtsIMutI | CAGTG | 4 cut(s) | 44, 145, 480, 600 |
| Cac8I | GCNNGC | 3 cut(s) | 187, 590, 607 |
| CciI | TCATGA | 1 cut(s) | 618 |
| Cfr13I | GGNCC | 3 cut(s) | 43, 111, 256 |
| Cfr9I | CCCGGG | 1 cut(s) | 395 |
| CviAII | CATG | 6 cut(s) | 100, 148, 253, 325, 528, 619 |
| CviJI | RGCY | 7 cut(s) | 112, 189, 287, 389, 526, 549, 648 |
| CviKI_1 | RGCY | 7 cut(s) | 112, 189, 287, 389, 526, 549, 648 |
| DdeI | CTNAG | 1 cut(s) | 265 |
| DpnI | GATC | 2 cut(s) | 302, 555 |
| DpnII | GATC | 2 cut(s) | 300, 553 |
| DraIII | CACNNNGTG | 3 cut(s) | 140, 327, 487 |
| EaeI | YGGCCR | 1 cut(s) | 387 |
| Eam1104I | CTCTTC | 1 cut(s) | 176 |
| EarI | CTCTTC | 1 cut(s) | 176 |
| Eco130I | CCWWGG | 1 cut(s) | 122 |
| Eco31I | GGTCTC | 1 cut(s) | 484 |
| Eco47I | GGWCC | 2 cut(s) | 43, 256 |
| Eco88I | CYCGRG | 1 cut(s) | 395 |
| EcoO109I | RGGNCCY | 1 cut(s) | 43 |
| EcoRII | CCWGG | 1 cut(s) | 334 |
| EcoT14I | CCWWGG | 1 cut(s) | 122 |
| ErhI | CCWWGG | 1 cut(s) | 122 |
| Esp3I | CGTCTC | 1 cut(s) | 197 |
| FaeI | CATG | 6 cut(s) | 103, 151, 256, 328, 531, 622 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 614, 646 |
| FaqI | GGGAC | 3 cut(s) | 56, 242, 317 |
| FatI | CATG | 6 cut(s) | 99, 147, 252, 324, 527, 618 |
| FbaI | TGATCA | 1 cut(s) | 553 |
| FblI | GTMKAC | 2 cut(s) | 293, 452 |
| FokI | GGATG | 1 cut(s) | 143 |
| HaeIII | GGCC | 2 cut(s) | 112, 389 |
| HapII | CCGG | 2 cut(s) | 309, 396 |
| Hin1II | CATG | 6 cut(s) | 103, 151, 256, 328, 531, 622 |
| HincII | GTYRAC | 1 cut(s) | 453 |
| HindII | GTYRAC | 1 cut(s) | 453 |
| HinfI | GANTC | 3 cut(s) | 151, 340, 506 |
| HpaII | CCGG | 2 cut(s) | 309, 396 |
| Hpy166II | GTNNAC | 2 cut(s) | 294, 453 |
| Hpy188I | TCNGA | 1 cut(s) | 268 |
| Hpy188III | TCNNGA | 2 cut(s) | 235, 619 |
| Hpy8I | GTNNAC | 2 cut(s) | 294, 453 |
| HpyAV | CCTTC | 2 cut(s) | 569, 616 |
| HpyCH4III | ACNGT | 5 cut(s) | 39, 218, 249, 433, 457 |
| HpyCH4IV | ACGT | 3 cut(s) | 60, 375, 426 |
| HpyCH4V | TGCA | 2 cut(s) | 569, 609 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 109, 175, 594 |
| HpyF3I | CTNAG | 1 cut(s) | 265 |
| HpySE526I | ACGT | 3 cut(s) | 60, 375, 426 |
| Hsp92II | CATG | 6 cut(s) | 103, 151, 256, 328, 531, 622 |
| KflI | GGGWCCC | 1 cut(s) | 43 |
| Ksp22I | TGATCA | 1 cut(s) | 553 |
| Kzo9I | GATC | 2 cut(s) | 300, 553 |
| LguI | GCTCTTC | 1 cut(s) | 176 |
| LmnI | GCTCC | 2 cut(s) | 523, 645 |
| LpnPI | CCDG | 9 cut(s) | 37, 291, 321, 322, 348, 409, 506, 507, 607 |
| LweI | GCATC | 1 cut(s) | 556 |
| MaeII | ACGT | 3 cut(s) | 60, 375, 426 |
| MaeIII | GTNAC | 4 cut(s) | 33, 235, 412, 427 |
| MalI | GATC | 2 cut(s) | 302, 555 |
| MboI | GATC | 2 cut(s) | 300, 553 |
| MboII | GAAGA | 3 cut(s) | 88, 163, 584 |
| MfeI | CAATTG | 1 cut(s) | 48 |
| MluCI | AATT | 1 cut(s) | 48 |
| MmeI | TCCRAC | 1 cut(s) | 488 |
| MnlI | CCTC | 2 cut(s) | 242, 471 |
| MseI | TTAA | 2 cut(s) | 164, 435 |
| MslI | CAYNNNNRTG | 1 cut(s) | 561 |
| MspI | CCGG | 2 cut(s) | 309, 396 |
| MspR9I | CCNGG | 4 cut(s) | 310, 336, 396, 397 |
| MunI | CAATTG | 1 cut(s) | 48 |
| Mva1269I | GAATGC | 1 cut(s) | 178 |
| MvaI | CCWGG | 1 cut(s) | 336 |
| MvnI | CGCG | 1 cut(s) | 32 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 109, 175, 594 |
| NciI | CCSGG | 3 cut(s) | 310, 396, 397 |
| NdeII | GATC | 2 cut(s) | 300, 553 |
| NlaIII | CATG | 6 cut(s) | 103, 151, 256, 328, 531, 622 |
| NlaIV | GGNNCC | 4 cut(s) | 44, 45, 258, 525 |
| NmeAIII | GCCGAG | 1 cut(s) | 88 |
| NmuCI | GTSAC | 3 cut(s) | 235, 412, 427 |
| PagI | TCATGA | 1 cut(s) | 618 |
| PasI | CCCWGGG | 1 cut(s) | 335 |
| PciSI | GCTCTTC | 1 cut(s) | 176 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 115 |
| PctI | GAATGC | 1 cut(s) | 178 |
| PfeI | GAWTC | 3 cut(s) | 151, 340, 506 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 642 |
| PpuMI | RGGWCCY | 1 cut(s) | 43 |
| Psp5II | RGGWCCY | 1 cut(s) | 43 |
| Psp6I | CCWGG | 1 cut(s) | 334 |
| PspGI | CCWGG | 1 cut(s) | 334 |
| PspN4I | GGNNCC | 4 cut(s) | 44, 45, 258, 525 |
| PspPI | GGNCC | 3 cut(s) | 43, 111, 256 |
| PspPPI | RGGWCCY | 1 cut(s) | 43 |
| RseI | CAYNNNNRTG | 1 cut(s) | 561 |
| SalI | GTCGAC | 1 cut(s) | 451 |
| SapI | GCTCTTC | 1 cut(s) | 176 |
| SaqAI | TTAA | 2 cut(s) | 164, 435 |
| Sau3AI | GATC | 2 cut(s) | 300, 553 |
| Sau96I | GGNCC | 3 cut(s) | 43, 111, 256 |
| ScrFI | CCNGG | 4 cut(s) | 310, 336, 396, 397 |
| SfaNI | GCATC | 1 cut(s) | 556 |
| SinI | GGWCC | 2 cut(s) | 43, 256 |
| SmaI | CCCGGG | 1 cut(s) | 397 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 561 |
| Sse9I | AATT | 1 cut(s) | 48 |
| SsiI | CCGC | 2 cut(s) | 30, 183 |
| SspI | AATATT | 1 cut(s) | 355 |
| StyD4I | CCNGG | 4 cut(s) | 308, 334, 394, 395 |
| StyI | CCWWGG | 1 cut(s) | 122 |
| TaaI | ACNGT | 5 cut(s) | 39, 218, 249, 433, 457 |
| TaiI | ACGT | 3 cut(s) | 63, 378, 429 |
| TaqI | TCGA | 1 cut(s) | 452 |
| TasI | AATT | 1 cut(s) | 48 |
| TfiI | GAWTC | 3 cut(s) | 151, 340, 506 |
| Tru1I | TTAA | 2 cut(s) | 164, 435 |
| Tru9I | TTAA | 2 cut(s) | 164, 435 |
| TscAI | CASTG | 4 cut(s) | 44, 145, 480, 607 |
| TseFI | GTSAC | 3 cut(s) | 235, 412, 427 |
| Tsp45I | GTSAC | 3 cut(s) | 235, 412, 427 |
| TspDTI | ATGAA | 6 cut(s) | 88, 191, 237, 241, 607, 635 |
| TspGWI | ACGGA | 1 cut(s) | 311 |
| TspMI | CCCGGG | 1 cut(s) | 395 |
| TspRI | CASTG | 4 cut(s) | 44, 145, 480, 607 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 642 |
| VpaK11BI | GGWCC | 2 cut(s) | 43, 256 |
| XmaI | CCCGGG | 1 cut(s) | 395 |
| XmiI | GTMKAC | 2 cut(s) | 293, 452 |