Rroxscaffold_7G00206850

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000590
Physical Location & Seq
Reverse (-)
56650183 .. 56651948
1766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00206850.1

Sequence Viewer

Length: 1056 bp
ATGGGAGAAGTTGACCCAGCTTTCATACAACACCCTGAACACAGGCCCAAACTCTCCATAATCGAAGCCGAAGGCATACCATTAATTGACCTCTCTGCAATAAACTCTCCAAACTCCATCTCTGACCCAGAAGATGTTGAAAGAATTGTTAGAGAAGTGGGCAATGCATGCAAGGAATGGGGGTTCTTCCAAGTAATCAACCATGGGCTGTCATCAGATAAGCTCCAAAAGATTGAGGCTTCTGCTAAAAAGTTCTTTGCTCTGCCTTTGGAGGAGAAGAGGAAGATTAGGAGAGACCTGAACAATGTGTTGGGGTACTTTGACACTGAGAACACCAAGAATATTCGGGACTGGAAGGAGGTGTTTGATTTTGATGTGGAGGACCCTACTTTAGTCCCAGCCTCGCCTGATCCTGAGGACGACGAAGAGACTGAGTGGACTAATCAATGGCCTGAGTATCCCCCAGAAATGAGGGAGGCATGTCAAGAATATGCTAGAGAAGTTGAAAAGCTAGCTCTAAAGTTGATGGGACTTATTGCCTTAAGTCTGGGCTTGCCGGAAGACAGGTTCAAAGGCTACTTCAAAGACCAAACCACTCGTATCAGACTCAATCACTATCCACCTTGCCCTTCCCCGGAGTTAGCCCTTGGTGTTGGTCGCCACAAGGATGGTGGCGCTCTAACTGTGCTAGCTCAAGATGATGTTGGAGGATTGGAAGTGAAGAGAAAAACAGATGGAGAGTGGGTTAGGGTAAATCCGACCCCAAATGCTTATATCATCAATCTTGGTGACACTCTTCAGGTTTGGAGCAATGAGAGATATGAGAGTACAGAACACAGGGTGATGGTGAATTCAGAGAAGGAAAGGTTTTCTATCCCGTACTTCCTTGAGCCAGCACACTACACCATAATAAAGCCCTTGGAAGAGCTGATCAATAAACAAAATCCTGCAAAGTATAGGCCATACAGCTGGGGCAAGTTTACGACTCACAGAAAGCTGAGTAATTTCAAGAAACTCAATGTTGAAAACATCCAAATTCATGATTTCAGGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

351

Amino Acids

40.56

Weight (kDa)

5.31

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 26 - 152 4.9e-33 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 201 - 298 4.2e-34 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19000 AT3G19000
fragaria_vesca FvH4_2g07071 FvH4_2g07080 FvH4_2g07080 FvH4_2g07081 FvH4_2g07082 FvH4_2g07082
malus_domestica MD05G1074000.v1.1 MD10G1088100.v1.1 MD10G1088200.v1.1 MD14G1207600.v1.1
prunus_persica Prupe.8G114100_v2.0.a1 Prupe.8G114200_v2.0.a1 Prupe.8G114300_v2.0.a1 Prupe.8G114500_v2.0.a1 Prupe.8G114800_v2.0.a1 Prupe.8G115100_v2.0.a1
pyrus_communis pycom05g06640 pycom05g06660 pycom05g06670 pycom10g07050
rosa_chinensis RchiOBHm_Chr6g0244141 RchiOBHm_Chr6g0259511 RchiOBHm_Chr6g0259521 RchiOBHm_Chr6g0259531 RchiOBHm_Chr6g0259551 RchiOBHm_Chr6g0259561
rosa_laevigata RLG00000014522 RLG00000014523 RLG00000014524 RLG00000014525 RLG00000014526 RLG00000014527
rosa_multiflora Rmu_ssc0000050.1_g000019 Rmu_ssc0000050.1_g000020 Rmu_ssc0000050.1_g000026 Rmu_ssc0000050.1_g000027 Rmu_ssc0000050.1_g000028
rosa_roxburghii Rroxscaffold_7G00206780 Rroxscaffold_7G00206790 Rroxscaffold_7G00206850 Rroxscaffold_7G00206860 Rroxscaffold_7G00206870 Rroxscaffold_7G00206880
rosa_rugosa Rorug05G0589100 Rorug05G0589200.1 Rorug05G0589300 Rorug05G0589400 Rorug05G0589500 Rorug05G0589600
rosa_samantha Rh4CG096000 Rh5BG231700 Rh6AG104900 Rh6AG105000 Rh6AG105100 Rh6AG105500 Rh6AG105600 Rh6BG098500 Rh6BG098700 Rh6BG098800 Rh6BG099000 Rh6BG099100 Rh6CG094200 Rh6CG094300 Rh6CG094400 Rh6CG094500 Rh6CG094600 Rh6DG088300 Rh6DG088400 Rh6DG088500 Rh6DG088600 Rh6DG088700
rosa_wichuraiana Rw0G014270 Rw0G014280 Rw0G014290 Rw6G009080 Rw6G009090 Rw6G009100 Rw6G009110 Rw6G009120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 404
AcsI RAATTY 2 cut(s) 850, 1035
AcuI CTGAAG 1 cut(s) 782
AdeI CACNNNGTG 1 cut(s) 841
AfaI GTAC 3 cut(s) 317, 829, 881
AfiI CCNNNNNNNGG 1 cut(s) 634
AflII CTTAAG 1 cut(s) 541
AgsI TTSAA 6 cut(s) 140, 506, 571, 583, 1009, 1025
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AloI GAACNNNNNNTCC 4 cut(s) 167, 199, 551, 583
AluBI AGCT 8 cut(s) 20, 223, 511, 515, 692, 928, 969, 997
AluI AGCT 8 cut(s) 20, 223, 511, 515, 692, 928, 969, 997
Alw26I GTCTC 2 cut(s) 288, 422
AlwI GGATC 1 cut(s) 404
AoxI GGCC 3 cut(s) 44, 449, 959
ApoI RAATTY 2 cut(s) 850, 1035
AseI ATTAAT 1 cut(s) 83
AspLEI GCGC 1 cut(s) 677
AspS9I GGNCC 2 cut(s) 45, 382
AsuC2I CCSGG 1 cut(s) 635
AsuHPI GGTGA 3 cut(s) 800, 853, 859
AsuNHI GCTAGC 2 cut(s) 511, 688
AvaII GGWCC 1 cut(s) 382
AxyI CCTNAGG 1 cut(s) 414
BbsI GAAGAC 1 cut(s) 567
BccI CCATC 5 cut(s) 125, 520, 662, 728, 838
BciVI GTATCC 1 cut(s) 468
BclI TGATCA 1 cut(s) 930
BcnI CCSGG 1 cut(s) 635
BcoDI GTCTC 2 cut(s) 288, 422
BfaI CTAG 3 cut(s) 495, 512, 689
BfoI RGCGCY 1 cut(s) 678
BfrI CTTAAG 1 cut(s) 541
BfuI GTATCC 1 cut(s) 468
Bme1390I CCNGG 1 cut(s) 635
Bme18I GGWCC 1 cut(s) 382
BmgT120I GGNCC 2 cut(s) 45, 382
BmiI GGNNCC 1 cut(s) 384
BmrFI CCNGG 1 cut(s) 635
BmtI GCTAGC 2 cut(s) 515, 692
BpiI GAAGAC 1 cut(s) 567
BpuEI CTTGAG 2 cut(s) 678, 908
BpuMI CCSGG 1 cut(s) 635
BsaI GGTCTC 1 cut(s) 288
BsaJI CCNNGG 4 cut(s) 202, 633, 646, 918
BsaXI ACNNNNNCTCC 2 cut(s) 729, 759
Bsc4I CCNNNNNNNGG 1 cut(s) 634
Bse1I ACTGG 1 cut(s) 356
Bse21I CCTNAGG 1 cut(s) 414
Bse3DI GCAATG 2 cut(s) 169, 817
BseDI CCNNGG 4 cut(s) 202, 633, 646, 918
BseGI GGATG 2 cut(s) 673, 1029
BseLI CCNNNNNNNGG 1 cut(s) 634
BseMI GCAATG 2 cut(s) 169, 817
BseMII CTCAG 5 cut(s) 318, 405, 423, 444, 989
BseNI ACTGG 1 cut(s) 356
BseRI GAGGAG 1 cut(s) 287
BseYI CCCAGC 3 cut(s) 16, 397, 969
BshFI GGCC 3 cut(s) 46, 451, 961
BsiSI CCGG 2 cut(s) 557, 635
BslFI GGGAC 3 cut(s) 362, 380, 543
BslI CCNNNNNNNGG 1 cut(s) 634
BsmAI GTCTC 2 cut(s) 288, 422
BsmFI GGGAC 3 cut(s) 362, 380, 543
BsnI GGCC 3 cut(s) 46, 451, 961
Bso31I GGTCTC 1 cut(s) 288
Bsp143I GATC 2 cut(s) 409, 930
Bsp19I CCATGG 1 cut(s) 202
BspANI GGCC 3 cut(s) 46, 451, 961
BspCNI CTCAG 5 cut(s) 319, 406, 424, 445, 990
BspHI TCATGA 1 cut(s) 1039
BspLI GGNNCC 1 cut(s) 384
BspOI GCTAGC 2 cut(s) 515, 692
BspPI GGATC 1 cut(s) 404
BspQI GCTCTTC 1 cut(s) 918
BspTI CTTAAG 1 cut(s) 541
BspTNI GGTCTC 1 cut(s) 288
BsrDI GCAATG 2 cut(s) 169, 817
BsrI ACTGG 1 cut(s) 356
BssECI CCNNGG 4 cut(s) 202, 633, 646, 918
BssMI GATC 2 cut(s) 409, 930
BssT1I CCWWGG 3 cut(s) 202, 646, 918
Bst4CI ACNGT 1 cut(s) 685
Bst6I CTCTTC 5 cut(s) 272, 420, 716, 801, 918
BstAFI CTTAAG 1 cut(s) 541
BstAPI GCANNNNNTGC 1 cut(s) 168
BstC8I GCNNGC 5 cut(s) 169, 513, 554, 690, 894
BstDEI CTNAG 5 cut(s) 327, 414, 432, 453, 998
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 2 cut(s) 673, 1029
BstH2I RGCGCY 1 cut(s) 678
BstHHI GCGC 1 cut(s) 677
BstKTI GATC 2 cut(s) 412, 933
BstMAI GTCTC 2 cut(s) 288, 422
BstMBI GATC 2 cut(s) 409, 930
BstMWI GCNNNNNNNGC 1 cut(s) 168
BstNSI RCATGY 2 cut(s) 171, 483
BstSCI CCNGG 1 cut(s) 633
BstV2I GAAGAC 1 cut(s) 567
BstXI CCANNNNNNTGG 2 cut(s) 668, 969
Bsu36I CCTNAGG 1 cut(s) 414
BsuI GTATCC 1 cut(s) 468
BsuRI GGCC 3 cut(s) 46, 451, 961
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 2 cut(s) 673, 1029
BtsIMutI CAGTG 1 cut(s) 324
Cac8I GCNNGC 5 cut(s) 169, 513, 554, 690, 894
CciI TCATGA 1 cut(s) 1039
CfoI GCGC 1 cut(s) 677
Cfr13I GGNCC 2 cut(s) 45, 382
Csp6I GTAC 3 cut(s) 316, 828, 880
CspCI CAANNNNNGTGG 2 cut(s) 652, 687
CviAII CATG 4 cut(s) 168, 203, 480, 1040
CviQI GTAC 3 cut(s) 316, 828, 880
DdeI CTNAG 5 cut(s) 327, 414, 432, 453, 998
DpnI GATC 2 cut(s) 411, 932
DpnII GATC 2 cut(s) 409, 930
DraIII CACNNNGTG 1 cut(s) 841
Eam1104I CTCTTC 5 cut(s) 272, 420, 716, 801, 918
EarI CTCTTC 5 cut(s) 272, 420, 716, 801, 918
Eco130I CCWWGG 3 cut(s) 202, 646, 918
Eco31I GGTCTC 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 782
Eco81I CCTNAGG 1 cut(s) 414
EcoO109I RGGNCCY 1 cut(s) 382
EcoRI GAATTC 1 cut(s) 850
EcoT14I CCWWGG 3 cut(s) 202, 646, 918
EcoT22I ATGCAT 1 cut(s) 169
ErhI CCWWGG 3 cut(s) 202, 646, 918
FaeI CATG 4 cut(s) 171, 206, 483, 1043
FaqI GGGAC 3 cut(s) 362, 380, 543
FatI CATG 4 cut(s) 167, 202, 479, 1039
FbaI TGATCA 1 cut(s) 930
FokI GGATG 2 cut(s) 680, 1016
FspBI CTAG 3 cut(s) 495, 512, 689
GlaI GCGC 1 cut(s) 676
GsaI CCCAGC 3 cut(s) 20, 401, 973
HaeII RGCGCY 1 cut(s) 678
HaeIII GGCC 3 cut(s) 46, 451, 961
HapII CCGG 2 cut(s) 557, 635
HhaI GCGC 1 cut(s) 677
Hin1II CATG 4 cut(s) 171, 206, 483, 1043
Hin6I GCGC 1 cut(s) 675
HinP1I GCGC 1 cut(s) 675
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 2 cut(s) 606, 985
HpaII CCGG 2 cut(s) 557, 635
HphI GGTGA 3 cut(s) 800, 853, 859
Hpy166II GTNNAC 3 cut(s) 13, 438, 981
Hpy188I TCNGA 5 cut(s) 124, 217, 605, 759, 856
Hpy188III TCNNGA 7 cut(s) 347, 413, 485, 695, 1009, 1040, 1048
Hpy8I GTNNAC 3 cut(s) 13, 438, 981
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 4 cut(s) 65, 349, 639, 853
HpyCH4III ACNGT 1 cut(s) 685
HpyCH4V TGCA 4 cut(s) 98, 167, 171, 950
HpyF10VI GCNNNNNNNGC 1 cut(s) 168
HpyF3I CTNAG 5 cut(s) 327, 414, 432, 453, 998
Hsp92II CATG 4 cut(s) 171, 206, 483, 1043
HspAI GCGC 1 cut(s) 675
Ksp22I TGATCA 1 cut(s) 930
Kzo9I GATC 2 cut(s) 409, 930
LguI GCTCTTC 1 cut(s) 918
LmnI GCTCC 2 cut(s) 228, 807
MaeI CTAG 3 cut(s) 495, 512, 689
MaeIII GTNAC 1 cut(s) 788
MalI GATC 2 cut(s) 411, 932
MboI GATC 2 cut(s) 409, 930
MboII GAAGA 9 cut(s) 143, 178, 289, 295, 437, 572, 733, 788, 935
MluCI AATT 5 cut(s) 84, 144, 850, 1003, 1035
MlyI GAGTC 2 cut(s) 600, 979
MmeI TCCRAC 2 cut(s) 685, 782
Mph1103I ATGCAT 1 cut(s) 169
MseI TTAA 2 cut(s) 83, 542
MslI CAYNNNNRTG 1 cut(s) 666
MspA1I CMGCKG 1 cut(s) 969
MspCI CTTAAG 1 cut(s) 541
MspI CCGG 2 cut(s) 557, 635
MspR9I CCNGG 1 cut(s) 635
MwoI GCNNNNNNNGC 1 cut(s) 168
NciI CCSGG 1 cut(s) 635
NcoI CCATGG 1 cut(s) 202
NdeII GATC 2 cut(s) 409, 930
NheI GCTAGC 2 cut(s) 511, 688
NlaIII CATG 4 cut(s) 171, 206, 483, 1043
NlaIV GGNNCC 1 cut(s) 384
NmuCI GTSAC 1 cut(s) 788
NsiI ATGCAT 1 cut(s) 169
NspI RCATGY 2 cut(s) 171, 483
PaeI GCATGC 1 cut(s) 171
PagI TCATGA 1 cut(s) 1039
PciSI GCTCTTC 1 cut(s) 918
PleI GAGTC 2 cut(s) 600, 979
PpsI GAGTC 2 cut(s) 600, 979
PpuMI RGGWCCY 1 cut(s) 382
PshBI ATTAAT 1 cut(s) 83
Psp5II RGGWCCY 1 cut(s) 382
PspFI CCCAGC 3 cut(s) 16, 397, 969
PspN4I GGNNCC 1 cut(s) 384
PspPI GGNCC 2 cut(s) 45, 382
PspPPI RGGWCCY 1 cut(s) 382
PvuII CAGCTG 1 cut(s) 969
RsaI GTAC 3 cut(s) 317, 829, 881
RsaNI GTAC 3 cut(s) 316, 828, 880
RseI CAYNNNNRTG 1 cut(s) 666
SapI GCTCTTC 1 cut(s) 918
SaqAI TTAA 2 cut(s) 83, 542
Sau3AI GATC 2 cut(s) 409, 930
Sau96I GGNCC 2 cut(s) 45, 382
SchI GAGTC 2 cut(s) 600, 979
ScrFI CCNGG 1 cut(s) 635
SinI GGWCC 1 cut(s) 382
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 3 cut(s) 541, 693, 887
SmoI CTYRAG 3 cut(s) 541, 693, 887
SphI GCATGC 1 cut(s) 171
Sse9I AATT 5 cut(s) 84, 144, 850, 1003, 1035
SspI AATATT 1 cut(s) 343
SspMI CTAG 3 cut(s) 495, 512, 689
StyD4I CCNGG 1 cut(s) 633
StyI CCWWGG 3 cut(s) 202, 646, 918
TaaI ACNGT 1 cut(s) 685
TaqI TCGA 1 cut(s) 63
TasI AATT 5 cut(s) 84, 144, 850, 1003, 1035
TatI WGTACW 1 cut(s) 827
Tru1I TTAA 2 cut(s) 83, 542
Tru9I TTAA 2 cut(s) 83, 542
TscAI CASTG 1 cut(s) 331
TseFI GTSAC 1 cut(s) 788
Tsp45I GTSAC 1 cut(s) 788
TspDTI ATGAA 2 cut(s) 13, 1028
TspRI CASTG 1 cut(s) 331
Vha464I CTTAAG 1 cut(s) 541
VpaK11BI GGWCC 1 cut(s) 382
VspI ATTAAT 1 cut(s) 83
XapI RAATTY 2 cut(s) 850, 1035
XceI RCATGY 2 cut(s) 171, 483
XcmI CCANNNNNNNNNTGG 1 cut(s) 668
XspI CTAG 3 cut(s) 495, 512, 689
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.