Rh6DG088300

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000590
Physical Location & Seq
Reverse (-)
10331767 .. 10334757
2991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG088300.1

Sequence Viewer

Length: 1422 bp
ATGGGAGAAGTTGACCCAGCTTTCATACAACACCCTGAACACAGGCCCAAACTCTCCATAATCGAAGCCGAAGGCATACCATTAATTGACCTCTCTGCAATAAACTCTCCAAACTCCATCTCTGACCCAGAAGATATTGAAAGAATTGTTAGAGAAGTGGGCAATGCATGCAAGGAATGGGGGTTCTTCCAAGTAATCAACCATGGGGTGTCATCAGATAAGCTCCAAAAGATTGAGGCTTCTGCTAAAAAGTTCTTTGCTCTGCCTTTGGAGGAGAAGAGGAAGATTAGGAGAGACCTGAACAATGTGTTGGGGTACTATGACACTGAGAACACCAAGAATATTCGGGACTGGAAGGAGGTGTTTGATTTTGATGTGGAGGACCCTACTTTAGTCCCAGCCTCACCTGATCCTGAGGACGACGAAGAGACTGAGTGGACTAATCAATGGCCTGAGTATCCCCCAGAAATGAGGGAGGCATGTCAAGAATATGCTCGAGAAGTTGAAAAGCTAGCTCTAAAGTTGATGGGACTTATTGCCTTAAGTCTGGGCTTGCCAGAAGACAGGTTCAAAGGCTACTTCAAAGACCAAACCACTCGTATCAGACTCAATCACTATCCACCTTGCCCTTCCCCGGAGTTAGCCCTTGGTGTTGGTCGCCACAAGGATGGTGGCGCTCTAACTGTGCTAGCTCAAGATGATGTTGGAGGATTGGAAGTGAAGAGAAAAACAGATGGAGAGTGGGTTAGGGTAAATCCCACCCCAAATGCTTATATCATCAATCTTGGTGACACTCTTCAGGTTTGGAGCAATGAGAGATATGAGAGTACAGAACACAGGGTGATGGTGAATTCAGAGAAGGAAAGGTTTTCTATCCCGTACTTCCTTGAGCCAGCACACTACACCATAATAAAGCCCTTGGAAGAGCTGATCAATGAACAAAATCCTGCAAAGTATAGGCCATACAGCTGGGGCAAGTTTACGACTCACAGAAAGCTGAGAATTGTTAGAGGAATAGGAAATCCATGCAAGGACTGGGGGTTCTTCCAAGTGATCAACCATGGAGTGCCGGTGGATAAGCTCCGAAAGATTGGGGCTGCTACTAGGAAGTTCTTTGCTCAGCCTTTGGAGGAGAAGAGGAAGAGTTGGAGGGATGAAACAAACGTGATAGGTTGCAATGAGAGAGAGAATACCCAGAATGTCAATGAGTACTGGAAGGAGGTGTTTAATTTAATAGTGGAAGAGCCTACCTTAGAGGAGAAGGAAGAGCATGAATGGACTGCCAATGGCCCAAGTATCCCCCAGAAATCAGTTGACTCAATCATCACTAAGGGTAGTAGACTAGTAGATGAATTATATACATTCCGTTTAGCTAATAACAATAGGAAGTTCCAAGAAACAGAAAATGAATCAAATGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

473

Amino Acids

54.65

Weight (kDa)

5.23

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 26 - 152 5.4e-33 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 201 - 298 7.7e-34 2OG-Fe(II) oxygenase superfamily
DIOX_N PF14226 331 - 422 3.9e-19 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19000 AT3G19000
fragaria_vesca FvH4_2g07071 FvH4_2g07080 FvH4_2g07080 FvH4_2g07081 FvH4_2g07082 FvH4_2g07082
malus_domestica MD05G1074000.v1.1 MD10G1088100.v1.1 MD10G1088200.v1.1 MD14G1207600.v1.1
prunus_persica Prupe.8G114100_v2.0.a1 Prupe.8G114200_v2.0.a1 Prupe.8G114300_v2.0.a1 Prupe.8G114500_v2.0.a1 Prupe.8G114800_v2.0.a1 Prupe.8G115100_v2.0.a1
pyrus_communis pycom05g06640 pycom05g06660 pycom05g06670 pycom10g07050
rosa_chinensis RchiOBHm_Chr6g0244141 RchiOBHm_Chr6g0259511 RchiOBHm_Chr6g0259521 RchiOBHm_Chr6g0259531 RchiOBHm_Chr6g0259551 RchiOBHm_Chr6g0259561
rosa_laevigata RLG00000014522 RLG00000014523 RLG00000014524 RLG00000014525 RLG00000014526 RLG00000014527
rosa_multiflora Rmu_ssc0000050.1_g000019 Rmu_ssc0000050.1_g000020 Rmu_ssc0000050.1_g000026 Rmu_ssc0000050.1_g000027 Rmu_ssc0000050.1_g000028
rosa_roxburghii Rroxscaffold_7G00206780 Rroxscaffold_7G00206790 Rroxscaffold_7G00206850 Rroxscaffold_7G00206860 Rroxscaffold_7G00206870 Rroxscaffold_7G00206880
rosa_rugosa Rorug05G0589100 Rorug05G0589200.1 Rorug05G0589300 Rorug05G0589400 Rorug05G0589500 Rorug05G0589600
rosa_samantha Rh4CG096000 Rh5BG231700 Rh6AG104900 Rh6AG105000 Rh6AG105100 Rh6AG105500 Rh6AG105600 Rh6BG098500 Rh6BG098700 Rh6BG098800 Rh6BG099000 Rh6BG099100 Rh6CG094200 Rh6CG094300 Rh6CG094400 Rh6CG094500 Rh6CG094600 Rh6DG088300 Rh6DG088400 Rh6DG088500 Rh6DG088600 Rh6DG088700
rosa_wichuraiana Rw0G014270 Rw0G014280 Rw0G014290 Rw6G009080 Rw6G009090 Rw6G009100 Rw6G009110 Rw6G009120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1339
AclWI GGATC 1 cut(s) 404
AcsI RAATTY 1 cut(s) 850
AcuI CTGAAG 1 cut(s) 782
AdeI CACNNNGTG 1 cut(s) 841
AfaI GTAC 4 cut(s) 317, 829, 881, 1211
AfiI CCNNNNNNNGG 1 cut(s) 634
AflII CTTAAG 1 cut(s) 541
AgsI TTSAA 4 cut(s) 140, 506, 571, 583
AhlI ACTAGT 1 cut(s) 1342
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AloI GAACNNNNNNTCC 4 cut(s) 167, 199, 1025, 1057
Alw26I GTCTC 2 cut(s) 288, 422
AlwI GGATC 1 cut(s) 404
Ama87I CYCGRG 1 cut(s) 495
AoxI GGCC 4 cut(s) 44, 449, 959, 1288
ApeKI GCWGC 1 cut(s) 1097
ApoI RAATTY 1 cut(s) 850
AseI ATTAAT 1 cut(s) 83
AspLEI GCGC 1 cut(s) 677
AspS9I GGNCC 3 cut(s) 45, 382, 1289
AsuC2I CCSGG 1 cut(s) 635
AsuHPI GGTGA 4 cut(s) 396, 800, 853, 859
AsuNHI GCTAGC 2 cut(s) 511, 688
AvaI CYCGRG 1 cut(s) 495
AvaII GGWCC 1 cut(s) 382
AxyI CCTNAGG 1 cut(s) 414
BbsI GAAGAC 1 cut(s) 567
BbvI GCAGC 1 cut(s) 1084
BccI CCATC 5 cut(s) 125, 520, 662, 728, 838
BciVI GTATCC 2 cut(s) 468, 1307
BclI TGATCA 2 cut(s) 930, 1053
BcnI CCSGG 1 cut(s) 635
BcoDI GTCTC 2 cut(s) 288, 422
BcuI ACTAGT 1 cut(s) 1342
BfaI CTAG 4 cut(s) 512, 689, 1104, 1343
BfoI RGCGCY 1 cut(s) 678
BfrI CTTAAG 1 cut(s) 541
BfuI GTATCC 2 cut(s) 468, 1307
BisI GCNGC 1 cut(s) 1098
BlpI GCTNAGC 1 cut(s) 1119
BlsI GCNGC 1 cut(s) 1099
BmcAI AGTACT 1 cut(s) 1211
Bme1390I CCNGG 1 cut(s) 635
Bme18I GGWCC 1 cut(s) 382
BmeT110I CYCGRG 1 cut(s) 495
BmgT120I GGNCC 3 cut(s) 45, 382, 1289
BmiI GGNNCC 1 cut(s) 384
BmrFI CCNGG 1 cut(s) 635
BmrI ACTGGG 1 cut(s) 1045
BmtI GCTAGC 2 cut(s) 515, 692
BmuI ACTGGG 1 cut(s) 1045
BpiI GAAGAC 1 cut(s) 567
Bpu1102I GCTNAGC 1 cut(s) 1119
BpuEI CTTGAG 2 cut(s) 678, 908
BpuMI CCSGG 1 cut(s) 635
BsaI GGTCTC 1 cut(s) 288
BsaJI CCNNGG 5 cut(s) 202, 633, 646, 918, 1060
BsaXI ACNNNNNCTCC 4 cut(s) 729, 759, 1056, 1086
Bsc4I CCNNNNNNNGG 1 cut(s) 634
Bse118I RCCGGY 1 cut(s) 1069
Bse1I ACTGG 3 cut(s) 356, 1040, 1217
Bse21I CCTNAGG 1 cut(s) 414
Bse3DI GCAATG 3 cut(s) 169, 817, 1183
BseDI CCNNGG 5 cut(s) 202, 633, 646, 918, 1060
BseGI GGATG 2 cut(s) 673, 1159
BseLI CCNNNNNNNGG 1 cut(s) 634
BseMI GCAATG 3 cut(s) 169, 817, 1183
BseMII CTCAG 6 cut(s) 318, 405, 423, 444, 989, 1133
BseNI ACTGG 3 cut(s) 356, 1040, 1217
BseRI GAGGAG 3 cut(s) 287, 1145, 1271
BseXI GCAGC 1 cut(s) 1084
BseYI CCCAGC 3 cut(s) 16, 397, 969
BshFI GGCC 4 cut(s) 46, 451, 961, 1290
BsiHKCI CYCGRG 1 cut(s) 495
BsiSI CCGG 2 cut(s) 635, 1070
BslFI GGGAC 3 cut(s) 362, 380, 543
BslI CCNNNNNNNGG 1 cut(s) 634
BsmAI GTCTC 2 cut(s) 288, 422
BsmFI GGGAC 3 cut(s) 362, 380, 543
BsnI GGCC 4 cut(s) 46, 451, 961, 1290
Bso31I GGTCTC 1 cut(s) 288
BsoBI CYCGRG 1 cut(s) 495
Bsp143I GATC 3 cut(s) 409, 930, 1053
Bsp1720I GCTNAGC 1 cut(s) 1119
Bsp19I CCATGG 2 cut(s) 202, 1060
BspANI GGCC 4 cut(s) 46, 451, 961, 1290
BspCNI CTCAG 6 cut(s) 319, 406, 424, 445, 990, 1132
BspLI GGNNCC 1 cut(s) 384
BspOI GCTAGC 2 cut(s) 515, 692
BspPI GGATC 1 cut(s) 404
BspQI GCTCTTC 3 cut(s) 918, 1236, 1260
BspTI CTTAAG 1 cut(s) 541
BspTNI GGTCTC 1 cut(s) 288
BsrDI GCAATG 3 cut(s) 169, 817, 1183
BsrFI RCCGGY 1 cut(s) 1069
BsrI ACTGG 3 cut(s) 356, 1040, 1217
BssAI RCCGGY 1 cut(s) 1069
BssECI CCNNGG 5 cut(s) 202, 633, 646, 918, 1060
BssMI GATC 3 cut(s) 409, 930, 1053
BssT1I CCWWGG 4 cut(s) 202, 646, 918, 1060
Bst4CI ACNGT 1 cut(s) 685
Bst6I CTCTTC 9 cut(s) 272, 420, 716, 801, 918, 1130, 1136, 1236, 1260
BstAFI CTTAAG 1 cut(s) 541
BstAPI GCANNNNNTGC 1 cut(s) 168
BstC8I GCNNGC 5 cut(s) 169, 513, 554, 690, 894
BstDEI CTNAG 8 cut(s) 327, 414, 432, 453, 998, 1119, 1252, 1329
BstDSI CCRYGG 2 cut(s) 202, 1060
BstF5I GGATG 2 cut(s) 673, 1159
BstH2I RGCGCY 1 cut(s) 678
BstHHI GCGC 1 cut(s) 677
BstKTI GATC 3 cut(s) 412, 933, 1056
BstMAI GTCTC 2 cut(s) 288, 422
BstMBI GATC 3 cut(s) 409, 930, 1053
BstMWI GCNNNNNNNGC 1 cut(s) 168
BstNSI RCATGY 2 cut(s) 171, 483
BstSCI CCNGG 1 cut(s) 633
BstV1I GCAGC 1 cut(s) 1084
BstV2I GAAGAC 1 cut(s) 567
BstXI CCANNNNNNTGG 2 cut(s) 668, 969
Bsu36I CCTNAGG 1 cut(s) 414
BsuI GTATCC 2 cut(s) 468, 1307
BsuRI GGCC 4 cut(s) 46, 451, 961, 1290
BtgI CCRYGG 2 cut(s) 202, 1060
BtsCI GGATG 2 cut(s) 673, 1159
BtsIMutI CAGTG 1 cut(s) 324
Cac8I GCNNGC 5 cut(s) 169, 513, 554, 690, 894
CfoI GCGC 1 cut(s) 677
Cfr10I RCCGGY 1 cut(s) 1069
Cfr13I GGNCC 3 cut(s) 45, 382, 1289
Csp6I GTAC 4 cut(s) 316, 828, 880, 1210
CspCI CAANNNNNGTGG 2 cut(s) 652, 687
CviAII CATG 6 cut(s) 168, 203, 480, 1026, 1061, 1271
CviQI GTAC 4 cut(s) 316, 828, 880, 1210
DdeI CTNAG 8 cut(s) 327, 414, 432, 453, 998, 1119, 1252, 1329
DpnI GATC 3 cut(s) 411, 932, 1055
DpnII GATC 3 cut(s) 409, 930, 1053
DraIII CACNNNGTG 1 cut(s) 841
Eam1104I CTCTTC 9 cut(s) 272, 420, 716, 801, 918, 1130, 1136, 1236, 1260
EarI CTCTTC 9 cut(s) 272, 420, 716, 801, 918, 1130, 1136, 1236, 1260
Eco130I CCWWGG 4 cut(s) 202, 646, 918, 1060
Eco31I GGTCTC 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 782
Eco81I CCTNAGG 1 cut(s) 414
Eco88I CYCGRG 1 cut(s) 495
EcoO109I RGGNCCY 1 cut(s) 382
EcoRI GAATTC 1 cut(s) 850
EcoT14I CCWWGG 4 cut(s) 202, 646, 918, 1060
EcoT22I ATGCAT 1 cut(s) 169
ErhI CCWWGG 4 cut(s) 202, 646, 918, 1060
FaeI CATG 6 cut(s) 171, 206, 483, 1029, 1064, 1274
FaqI GGGAC 3 cut(s) 362, 380, 543
FatI CATG 6 cut(s) 167, 202, 479, 1025, 1060, 1270
FbaI TGATCA 2 cut(s) 930, 1053
FblI GTMKAC 1 cut(s) 1339
Fnu4HI GCNGC 1 cut(s) 1098
FokI GGATG 2 cut(s) 680, 1166
Fsp4HI GCNGC 1 cut(s) 1098
FspBI CTAG 4 cut(s) 512, 689, 1104, 1343
GlaI GCGC 1 cut(s) 676
GluI GCNGC 1 cut(s) 1098
GsaI CCCAGC 3 cut(s) 20, 401, 973
HaeII RGCGCY 1 cut(s) 678
HaeIII GGCC 4 cut(s) 46, 451, 961, 1290
HapII CCGG 2 cut(s) 635, 1070
HhaI GCGC 1 cut(s) 677
Hin1II CATG 6 cut(s) 171, 206, 483, 1029, 1064, 1274
Hin6I GCGC 1 cut(s) 675
HinP1I GCGC 1 cut(s) 675
HincII GTYRAC 2 cut(s) 13, 1315
HindII GTYRAC 2 cut(s) 13, 1315
HinfI GANTC 4 cut(s) 606, 985, 1316, 1409
HpaII CCGG 2 cut(s) 635, 1070
HphI GGTGA 4 cut(s) 396, 800, 853, 859
Hpy166II GTNNAC 5 cut(s) 13, 438, 981, 1315, 1340
Hpy188I TCNGA 5 cut(s) 124, 217, 605, 856, 1085
Hpy188III TCNNGA 5 cut(s) 347, 413, 485, 497, 695
Hpy8I GTNNAC 5 cut(s) 13, 438, 981, 1315, 1340
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 6 cut(s) 65, 349, 639, 853, 1210, 1255
HpyCH4III ACNGT 1 cut(s) 685
HpyCH4IV ACGT 1 cut(s) 1164
HpyCH4V TGCA 6 cut(s) 98, 167, 171, 950, 1029, 1176
HpyF10VI GCNNNNNNNGC 1 cut(s) 168
HpyF3I CTNAG 8 cut(s) 327, 414, 432, 453, 998, 1119, 1252, 1329
HpySE526I ACGT 1 cut(s) 1164
Hsp92II CATG 6 cut(s) 171, 206, 483, 1029, 1064, 1274
HspAI GCGC 1 cut(s) 675
Ksp22I TGATCA 2 cut(s) 930, 1053
Kzo9I GATC 3 cut(s) 409, 930, 1053
LguI GCTCTTC 3 cut(s) 918, 1236, 1260
LmnI GCTCC 3 cut(s) 228, 807, 1086
Lsp1109I GCAGC 1 cut(s) 1084
MaeI CTAG 4 cut(s) 512, 689, 1104, 1343
MaeII ACGT 1 cut(s) 1164
MaeIII GTNAC 1 cut(s) 788
MalI GATC 3 cut(s) 411, 932, 1055
MboI GATC 3 cut(s) 409, 930, 1053
MluCI AATT 6 cut(s) 84, 144, 850, 1002, 1228, 1352
MlyI GAGTC 3 cut(s) 600, 979, 1310
MmeI TCCRAC 2 cut(s) 685, 1127
Mph1103I ATGCAT 1 cut(s) 169
MseI TTAA 4 cut(s) 83, 542, 1227, 1232
MslI CAYNNNNRTG 1 cut(s) 666
MspA1I CMGCKG 1 cut(s) 969
MspCI CTTAAG 1 cut(s) 541
MspI CCGG 2 cut(s) 635, 1070
MspR9I CCNGG 1 cut(s) 635
MwoI GCNNNNNNNGC 1 cut(s) 168
NciI CCSGG 1 cut(s) 635
NcoI CCATGG 2 cut(s) 202, 1060
NdeII GATC 3 cut(s) 409, 930, 1053
NheI GCTAGC 2 cut(s) 511, 688
NlaIII CATG 6 cut(s) 171, 206, 483, 1029, 1064, 1274
NlaIV GGNNCC 1 cut(s) 384
NmuCI GTSAC 1 cut(s) 788
NsiI ATGCAT 1 cut(s) 169
NspI RCATGY 2 cut(s) 171, 483
PaeI GCATGC 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 495
PciSI GCTCTTC 3 cut(s) 918, 1236, 1260
PfeI GAWTC 1 cut(s) 1409
PkrI GCNGC 1 cut(s) 1099
PleI GAGTC 3 cut(s) 600, 979, 1310
PpsI GAGTC 3 cut(s) 600, 979, 1310
PpuMI RGGWCCY 1 cut(s) 382
PshBI ATTAAT 1 cut(s) 83
Psp5II RGGWCCY 1 cut(s) 382
PspFI CCCAGC 3 cut(s) 16, 397, 969
PspN4I GGNNCC 1 cut(s) 384
PspPI GGNCC 3 cut(s) 45, 382, 1289
PspPPI RGGWCCY 1 cut(s) 382
PvuII CAGCTG 1 cut(s) 969
RsaI GTAC 4 cut(s) 317, 829, 881, 1211
RsaNI GTAC 4 cut(s) 316, 828, 880, 1210
RseI CAYNNNNRTG 1 cut(s) 666
SapI GCTCTTC 3 cut(s) 918, 1236, 1260
SaqAI TTAA 4 cut(s) 83, 542, 1227, 1232
SatI GCNGC 1 cut(s) 1098
Sau3AI GATC 3 cut(s) 409, 930, 1053
Sau96I GGNCC 3 cut(s) 45, 382, 1289
ScaI AGTACT 1 cut(s) 1211
SchI GAGTC 3 cut(s) 600, 979, 1310
ScrFI CCNGG 1 cut(s) 635
Sfr274I CTCGAG 1 cut(s) 495
SinI GGWCC 1 cut(s) 382
SlaI CTCGAG 1 cut(s) 495
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 4 cut(s) 495, 541, 693, 887
SmoI CTYRAG 4 cut(s) 495, 541, 693, 887
SpeI ACTAGT 1 cut(s) 1342
SphI GCATGC 1 cut(s) 171
Sse9I AATT 6 cut(s) 84, 144, 850, 1002, 1228, 1352
SspI AATATT 1 cut(s) 343
SspMI CTAG 4 cut(s) 512, 689, 1104, 1343
StyD4I CCNGG 1 cut(s) 633
StyI CCWWGG 4 cut(s) 202, 646, 918, 1060
TaaI ACNGT 1 cut(s) 685
TaiI ACGT 1 cut(s) 1167
TaqI TCGA 2 cut(s) 63, 496
TasI AATT 6 cut(s) 84, 144, 850, 1002, 1228, 1352
TatI WGTACW 2 cut(s) 827, 1209
TfiI GAWTC 1 cut(s) 1409
Tru1I TTAA 4 cut(s) 83, 542, 1227, 1232
Tru9I TTAA 4 cut(s) 83, 542, 1227, 1232
TscAI CASTG 1 cut(s) 331
TseFI GTSAC 1 cut(s) 788
TseI GCWGC 1 cut(s) 1097
Tsp45I GTSAC 1 cut(s) 788
TspDTI ATGAA 6 cut(s) 13, 951, 1170, 1287, 1365, 1422
TspGWI ACGGA 1 cut(s) 1355
TspRI CASTG 1 cut(s) 331
Vha464I CTTAAG 1 cut(s) 541
VpaK11BI GGWCC 1 cut(s) 382
VspI ATTAAT 1 cut(s) 83
XapI RAATTY 1 cut(s) 850
XceI RCATGY 2 cut(s) 171, 483
XcmI CCANNNNNNNNNTGG 2 cut(s) 668, 1032
XhoI CTCGAG 1 cut(s) 495
XmiI GTMKAC 1 cut(s) 1339
XspI CTAG 4 cut(s) 512, 689, 1104, 1343
ZrmI AGTACT 1 cut(s) 1211
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.