Rroxscaffold_7G00206790

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000590
Physical Location & Seq
Reverse (-)
56535170 .. 56537016
1847 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00206790.1

Sequence Viewer

Length: 1062 bp
ATGGGAGAAGTTGATCCAGTTTTCATCCAAGACCCTGAACACAGGCCTAAACTCTCCATCATTGAACTCGAAGGCCTACCATTGATAGACCTCTCTCCATTAAGCTCCCCAGACTCCATTTCTGACCCTAAAGCCATTGAAGTGCTTGTTAGAGAAATAGGCAATGCATGCAAGAACTGGGGCTTCTTCCAATTGATCAACCATGGAGTGCCACTGGAAAAGCGAGAGAAGATTGAAATGGCAGCCAGGAAGTTCTTTGCCCAGCCTTTGGAGGAGAAGAGGAAGATTAGGAGGGATGAAATACGTATGGTAGGTTACTTTGACACTGAGCATACCAAAAATGTTAGAGACTGGAAGGAGGTGCTTGAATTCCTTGTGGAGGAGCCTACATTAGTTCCCTCCTCGACTGAGCCTGATGACAAGGAGGAGACACAGTGGTTTAATCAATGGCCTGAGAACCCTCCTGAATTAAGGGAGGTGCTTGAAGAATATTCTGAAGAAGTAGAAAAGCTATCTCTAAAGTTGGTGGGACTAATTGCATTGAGCCTAGGCTTGCCAGAAGACAGGTTCAAAGGCTACTTCAAAGACCAAACCAGTTTTATCAGACTGAATCACTATCCACCTTGCCCTTCTCCTCAGTTAGCACTCGGTGTTGGGCCCCACAAGGATGATGGTGCCTTAACTGTATTGGCTCAAGATGAGGTTGGAGGATTGGAGGTGAAGCGAAAAACAGATGGAGAGTGGATTGGGGTCAGACCTACACCAAATGCCTATATCATCAATGTTGGTGACATTTTTCAGGTATGGAGCAACGATAAATATGAGAGTGTGGAACATAGGGTGATGGTAAACTCAGAGAAGGAAAGGTTTTCTGTTCCCTTCTTTCTCAACCCAGCACACTACACAGAAGTGAAGCCTTTGGAGGAGCTCACCAATAAACAAAACCCTGCCAAGTATAGGCCTCATAACTGGGGCAAGTTTCTGAATCTCAGAAAACTCAGTAATTTCAAGAAACTCAAAGCTGAAAACATCGAAATCTATTATTTCAGGGTTTCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

353

Amino Acids

40.93

Weight (kDa)

5.34

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 26 - 153 1.9e-31 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 199 - 298 1.3e-34 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19000 AT3G19000
fragaria_vesca FvH4_2g07071 FvH4_2g07080 FvH4_2g07080 FvH4_2g07081 FvH4_2g07082 FvH4_2g07082
malus_domestica MD05G1074000.v1.1 MD10G1088100.v1.1 MD10G1088200.v1.1 MD14G1207600.v1.1
prunus_persica Prupe.8G114100_v2.0.a1 Prupe.8G114200_v2.0.a1 Prupe.8G114300_v2.0.a1 Prupe.8G114500_v2.0.a1 Prupe.8G114800_v2.0.a1 Prupe.8G115100_v2.0.a1
pyrus_communis pycom05g06640 pycom05g06660 pycom05g06670 pycom10g07050
rosa_chinensis RchiOBHm_Chr6g0244141 RchiOBHm_Chr6g0259511 RchiOBHm_Chr6g0259521 RchiOBHm_Chr6g0259531 RchiOBHm_Chr6g0259551 RchiOBHm_Chr6g0259561
rosa_laevigata RLG00000014522 RLG00000014523 RLG00000014524 RLG00000014525 RLG00000014526 RLG00000014527
rosa_multiflora Rmu_ssc0000050.1_g000019 Rmu_ssc0000050.1_g000020 Rmu_ssc0000050.1_g000026 Rmu_ssc0000050.1_g000027 Rmu_ssc0000050.1_g000028
rosa_roxburghii Rroxscaffold_7G00206780 Rroxscaffold_7G00206790 Rroxscaffold_7G00206850 Rroxscaffold_7G00206860 Rroxscaffold_7G00206870 Rroxscaffold_7G00206880
rosa_rugosa Rorug05G0589100 Rorug05G0589200.1 Rorug05G0589300 Rorug05G0589400 Rorug05G0589500 Rorug05G0589600
rosa_samantha Rh4CG096000 Rh5BG231700 Rh6AG104900 Rh6AG105000 Rh6AG105100 Rh6AG105500 Rh6AG105600 Rh6BG098500 Rh6BG098700 Rh6BG098800 Rh6BG099000 Rh6BG099100 Rh6CG094200 Rh6CG094300 Rh6CG094400 Rh6CG094500 Rh6CG094600 Rh6DG088300 Rh6DG088400 Rh6DG088500 Rh6DG088600 Rh6DG088700
rosa_wichuraiana Rw0G014270 Rw0G014280 Rw0G014290 Rw6G009080 Rw6G009090 Rw6G009100 Rw6G009110 Rw6G009120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 674
AccB7I CCANNNNNTGG 1 cut(s) 268
AclWI GGATC 1 cut(s) 8
AcsI RAATTY 1 cut(s) 368
AcuI CTGAAG 1 cut(s) 516
AdeI CACNNNGTG 1 cut(s) 650
AfiI CCNNNNNNNGG 3 cut(s) 268, 379, 957
AgsI TTSAA 9 cut(s) 65, 140, 236, 368, 485, 571, 583, 1009, 1056
AjnI CCWGG 1 cut(s) 245
AleI CACNNNNGTG 1 cut(s) 908
AluBI AGCT 4 cut(s) 105, 511, 928, 1022
AluI AGCT 4 cut(s) 105, 511, 928, 1022
Alw21I GWGCWC 1 cut(s) 930
Alw26I GTCTC 2 cut(s) 342, 422
AlwI GGATC 1 cut(s) 8
AoxI GGCC 5 cut(s) 44, 73, 449, 656, 959
ApaI GGGCCC 1 cut(s) 660
ApeKI GCWGC 1 cut(s) 242
ApoI RAATTY 1 cut(s) 368
AspA2I CCTAGG 1 cut(s) 547
AspS9I GGNCC 2 cut(s) 656, 657
AsuHPI GGTGA 4 cut(s) 730, 800, 853, 922
AvrII CCTAGG 1 cut(s) 547
BaeGI GKGCMC 1 cut(s) 660
BanI GGYRCC 1 cut(s) 674
BanII GRGCYC 2 cut(s) 660, 930
BbsI GAAGAC 1 cut(s) 567
Bbv12I GWGCWC 1 cut(s) 930
BbvI GCAGC 1 cut(s) 254
BccI CCATC 4 cut(s) 65, 665, 728, 838
BciT130I CCWGG 1 cut(s) 247
BclI TGATCA 1 cut(s) 195
BcoDI GTCTC 2 cut(s) 342, 422
BfaI CTAG 1 cut(s) 548
BisI GCNGC 1 cut(s) 243
BlnI CCTAGG 1 cut(s) 547
BlsI GCNGC 1 cut(s) 244
Bme1390I CCNGG 1 cut(s) 247
BmgT120I GGNCC 2 cut(s) 656, 657
BmiI GGNNCC 4 cut(s) 384, 658, 659, 676
BmrFI CCNGG 1 cut(s) 247
BmrI ACTGGG 2 cut(s) 187, 979
BmuI ACTGGG 2 cut(s) 187, 979
BpiI GAAGAC 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 678
BsaAI YACGTR 1 cut(s) 305
BsaJI CCNNGG 2 cut(s) 202, 547
BsaXI ACNNNNNCTCC 2 cut(s) 419, 449
Bsc4I CCNNNNNNNGG 3 cut(s) 268, 379, 957
Bse1I ACTGG 6 cut(s) 17, 182, 219, 356, 594, 974
Bse3DI GCAATG 1 cut(s) 169
BseBI CCWGG 1 cut(s) 247
BseDI CCNNGG 2 cut(s) 202, 547
BseGI GGATG 3 cut(s) 24, 301, 673
BseLI CCNNNNNNNGG 3 cut(s) 268, 379, 957
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 7 cut(s) 318, 399, 444, 650, 867, 1003, 1012
BseNI ACTGG 6 cut(s) 17, 182, 219, 356, 594, 974
BseRI GAGGAG 6 cut(s) 287, 391, 395, 440, 624, 938
BseSI GKGCMC 1 cut(s) 660
BseXI GCAGC 1 cut(s) 254
BseYI CCCAGC 2 cut(s) 261, 892
BshFI GGCC 5 cut(s) 46, 75, 451, 658, 961
BshNI GGYRCC 1 cut(s) 674
BsiHKAI GWGCWC 1 cut(s) 930
BslFI GGGAC 1 cut(s) 543
BslI CCNNNNNNNGG 3 cut(s) 268, 379, 957
BsmAI GTCTC 2 cut(s) 342, 422
BsmFI GGGAC 1 cut(s) 543
BsnI GGCC 5 cut(s) 46, 75, 451, 658, 961
Bsp120I GGGCCC 1 cut(s) 656
Bsp1286I GDGCHC 2 cut(s) 660, 930
Bsp143I GATC 2 cut(s) 13, 195
Bsp19I CCATGG 1 cut(s) 202
BspANI GGCC 5 cut(s) 46, 75, 451, 658, 961
BspCNI CTCAG 7 cut(s) 319, 400, 445, 649, 866, 1002, 1011
BspLI GGNNCC 4 cut(s) 384, 658, 659, 676
BspPI GGATC 1 cut(s) 8
BspT107I GGYRCC 1 cut(s) 674
BsrDI GCAATG 1 cut(s) 169
BsrI ACTGG 6 cut(s) 17, 182, 219, 356, 594, 974
BssECI CCNNGG 2 cut(s) 202, 547
BssMI GATC 2 cut(s) 13, 195
BssT1I CCWWGG 2 cut(s) 202, 547
Bst2UI CCWGG 1 cut(s) 247
Bst4CI ACNGT 2 cut(s) 435, 685
Bst6I CTCTTC 1 cut(s) 272
BstAPI GCANNNNNTGC 1 cut(s) 168
BstBAI YACGTR 1 cut(s) 305
BstC8I GCNNGC 2 cut(s) 169, 554
BstDEI CTNAG 7 cut(s) 327, 408, 453, 636, 853, 989, 998
BstDSI CCRYGG 1 cut(s) 202
BstENI CCTNNNNNAGG 1 cut(s) 377
BstF5I GGATG 3 cut(s) 24, 301, 673
BstKTI GATC 2 cut(s) 16, 198
BstMAI GTCTC 2 cut(s) 342, 422
BstMBI GATC 2 cut(s) 13, 195
BstMWI GCNNNNNNNGC 1 cut(s) 168
BstNI CCWGG 1 cut(s) 247
BstNSI RCATGY 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 245
BstSLI GKGCMC 1 cut(s) 660
BstSNI TACGTA 1 cut(s) 305
BstV1I GCAGC 1 cut(s) 254
BstV2I GAAGAC 1 cut(s) 567
BsuRI GGCC 5 cut(s) 46, 75, 451, 658, 961
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 3 cut(s) 24, 301, 673
BtsIMutI CAGTG 3 cut(s) 212, 324, 440
Cac8I GCNNGC 2 cut(s) 169, 554
Cfr13I GGNCC 2 cut(s) 656, 657
CviAII CATG 2 cut(s) 168, 203
DdeI CTNAG 7 cut(s) 327, 408, 453, 636, 853, 989, 998
DpnI GATC 2 cut(s) 15, 197
DpnII GATC 2 cut(s) 13, 195
DraIII CACNNNGTG 1 cut(s) 650
Eam1104I CTCTTC 1 cut(s) 272
EarI CTCTTC 1 cut(s) 272
Ecl136II GAGCTC 1 cut(s) 928
Eco105I TACGTA 1 cut(s) 305
Eco130I CCWWGG 2 cut(s) 202, 547
Eco147I AGGCCT 3 cut(s) 46, 75, 961
Eco24I GRGCYC 2 cut(s) 660, 930
Eco53kI GAGCTC 1 cut(s) 928
Eco57I CTGAAG 1 cut(s) 516
EcoICRI GAGCTC 1 cut(s) 928
EcoNI CCTNNNNNAGG 1 cut(s) 377
EcoO109I RGGNCCY 1 cut(s) 657
EcoRI GAATTC 1 cut(s) 368
EcoRII CCWGG 1 cut(s) 245
EcoT14I CCWWGG 2 cut(s) 202, 547
EcoT22I ATGCAT 1 cut(s) 169
EcoT38I GRGCYC 2 cut(s) 660, 930
ErhI CCWWGG 2 cut(s) 202, 547
FaeI CATG 2 cut(s) 171, 206
FaqI GGGAC 1 cut(s) 543
FatI CATG 2 cut(s) 167, 202
FbaI TGATCA 1 cut(s) 195
Fnu4HI GCNGC 1 cut(s) 243
FokI GGATG 3 cut(s) 11, 308, 680
FriOI GRGCYC 2 cut(s) 660, 930
Fsp4HI GCNGC 1 cut(s) 243
FspBI CTAG 1 cut(s) 548
GluI GCNGC 1 cut(s) 243
GsaI CCCAGC 2 cut(s) 265, 896
HaeIII GGCC 5 cut(s) 46, 75, 451, 658, 961
Hin1II CATG 2 cut(s) 171, 206
HinfI GANTC 3 cut(s) 113, 610, 985
HphI GGTGA 4 cut(s) 730, 800, 853, 922
Hpy166II GTNNAC 1 cut(s) 850
Hpy188I TCNGA 7 cut(s) 124, 496, 605, 755, 856, 984, 992
Hpy188III TCNNGA 3 cut(s) 464, 695, 1009
Hpy8I GTNNAC 1 cut(s) 850
HpyAV CCTTC 5 cut(s) 65, 349, 639, 853, 889
HpyCH4III ACNGT 2 cut(s) 435, 685
HpyCH4IV ACGT 1 cut(s) 304
HpyCH4V TGCA 3 cut(s) 167, 171, 539
HpyF10VI GCNNNNNNNGC 1 cut(s) 168
HpyF3I CTNAG 7 cut(s) 327, 408, 453, 636, 853, 989, 998
HpySE526I ACGT 1 cut(s) 304
Hsp92II CATG 2 cut(s) 171, 206
Ksp22I TGATCA 1 cut(s) 195
Kzo9I GATC 2 cut(s) 13, 195
LmnI GCTCC 4 cut(s) 110, 382, 807, 925
Lsp1109I GCAGC 1 cut(s) 254
MaeI CTAG 1 cut(s) 548
MaeII ACGT 1 cut(s) 304
MaeIII GTNAC 2 cut(s) 314, 788
MalI GATC 2 cut(s) 15, 197
MboI GATC 2 cut(s) 13, 195
MboII GAAGA 7 cut(s) 178, 241, 289, 295, 497, 509, 572
MfeI CAATTG 1 cut(s) 191
MhlI GDGCHC 2 cut(s) 660, 930
MluCI AATT 6 cut(s) 191, 368, 467, 534, 1003, 1057
MlyI GAGTC 1 cut(s) 107
MmeI TCCRAC 1 cut(s) 685
Mph1103I ATGCAT 1 cut(s) 169
MseI TTAA 5 cut(s) 101, 441, 470, 680, 1060
MslI CAYNNNNRTG 3 cut(s) 140, 666, 908
MspR9I CCNGG 1 cut(s) 247
MunI CAATTG 1 cut(s) 191
MvaI CCWGG 1 cut(s) 247
MwoI GCNNNNNNNGC 1 cut(s) 168
NcoI CCATGG 1 cut(s) 202
NdeII GATC 2 cut(s) 13, 195
NlaIII CATG 2 cut(s) 171, 206
NlaIV GGNNCC 4 cut(s) 384, 658, 659, 676
NmuCI GTSAC 1 cut(s) 788
NsiI ATGCAT 1 cut(s) 169
NspI RCATGY 1 cut(s) 171
OliI CACNNNNGTG 1 cut(s) 908
PaeI GCATGC 1 cut(s) 171
PceI AGGCCT 3 cut(s) 46, 75, 961
PfeI GAWTC 2 cut(s) 610, 985
PflMI CCANNNNNTGG 1 cut(s) 268
PkrI GCNGC 1 cut(s) 244
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
Ppu21I YACGTR 1 cut(s) 305
Psp124BI GAGCTC 1 cut(s) 930
Psp6I CCWGG 1 cut(s) 245
PspFI CCCAGC 2 cut(s) 261, 892
PspGI CCWGG 1 cut(s) 245
PspN4I GGNNCC 4 cut(s) 384, 658, 659, 676
PspOMI GGGCCC 1 cut(s) 656
PspPI GGNCC 2 cut(s) 656, 657
RseI CAYNNNNRTG 3 cut(s) 140, 666, 908
SacI GAGCTC 1 cut(s) 930
SaqAI TTAA 5 cut(s) 101, 441, 470, 680, 1060
SatI GCNGC 1 cut(s) 243
Sau3AI GATC 2 cut(s) 13, 195
Sau96I GGNCC 2 cut(s) 656, 657
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 1 cut(s) 247
SduI GDGCHC 2 cut(s) 660, 930
SmiMI CAYNNNNRTG 3 cut(s) 140, 666, 908
SmlI CTYRAG 1 cut(s) 693
SmoI CTYRAG 1 cut(s) 693
SnaBI TACGTA 1 cut(s) 305
SphI GCATGC 1 cut(s) 171
Sse9I AATT 6 cut(s) 191, 368, 467, 534, 1003, 1057
SseBI AGGCCT 3 cut(s) 46, 75, 961
SspI AATATT 1 cut(s) 491
SspMI CTAG 1 cut(s) 548
SstI GAGCTC 1 cut(s) 930
StuI AGGCCT 3 cut(s) 46, 75, 961
StyD4I CCNGG 1 cut(s) 245
StyI CCWWGG 2 cut(s) 202, 547
TaaI ACNGT 2 cut(s) 435, 685
TaiI ACGT 1 cut(s) 307
TaqI TCGA 3 cut(s) 69, 404, 1032
TasI AATT 6 cut(s) 191, 368, 467, 534, 1003, 1057
TfiI GAWTC 2 cut(s) 610, 985
Tru1I TTAA 5 cut(s) 101, 441, 470, 680, 1060
Tru9I TTAA 5 cut(s) 101, 441, 470, 680, 1060
TscAI CASTG 3 cut(s) 219, 331, 440
TseFI GTSAC 1 cut(s) 788
TseI GCWGC 1 cut(s) 242
Tsp45I GTSAC 1 cut(s) 788
TspDTI ATGAA 2 cut(s) 13, 312
TspRI CASTG 3 cut(s) 219, 331, 440
Van91I CCANNNNNTGG 1 cut(s) 268
XagI CCTNNNNNAGG 1 cut(s) 377
XapI RAATTY 1 cut(s) 368
XceI RCATGY 1 cut(s) 171
XcmI CCANNNNNNNNNTGG 1 cut(s) 668
XmaJI CCTAGG 1 cut(s) 547
XspI CTAG 1 cut(s) 548
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.