Rh6BG099000

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000590
Physical Location & Seq
Reverse (-)
15409972 .. 15411514
1543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG099000.1

Sequence Viewer

Length: 1062 bp
ATGGGAGAAGTTGATCCAGCTTTCATCCAAGACCCTGAACACAGACCTAAACTCTCCGTCATCGAACCTGAAGGCATACCATTGATAGACCTCTCTCCATTAAGCTCCCCAGACTCCATTTCTGACCCTAAAGCCATTGAAGTGCTTGTTAGAGAAATAGGCAATGCATGCAAGAACTGGGGCTTCTTCCAAGTGATCAACCATGGAGTGCCACAGGAAAAGCTCAAGAAGGTTGAGACTGCGGCCAGGAAGTTCTTTGCCCAGCCTTTGGAGGAGAAGAGGAAGATTAGGAGGGATGAAATACGTGTGGTAGGTTACTTTGACACTGAGCATACCAAAAATGTTAGAGACTGGAAGGAGGTGTTTGATTTCCTTGTGGAGGAGCCTACATTAGTTCCCTCCTCGACTGAGCCTGATGACAAGGGGGAGACACAGTGGTTTAATCAATGGCCTGAGAACCCTCCTGAATTAAGGGAGGTGCTTGAAGAATATTCTCAAGAAGTAGAAAAGCTATCTCTAAAGTTGATGGGACTAATTGCGTTGAGCCTAGGCTTGCCAGAAGACAGGTTCAAAGGCTACTTCAAAGACCAAACCAGTTTTATCAGACTCAATTACTATCCACCTTGCCCTTCCCCTCAGTTAGCACTCGGTGTTGGTCCCCACAAGGATGCCGGTGCCTTAACTGTACTGGCTCAAGATGAGGTTGGAGGATTGGAGGTGAAGCGAAAAACAGATGGAGAGTGGATTCGGGTCAGACCTACACCAAATGCCTATATCATCAATGTTGGTGACATTCTTCAGGTATGGAGCAACGATAAATATGAGAGTGCGGAACATAGGGTGATGGTAAACGCAGAGAAGGAAAGGTTTTCTGTTCCCTTCCTTCTCTTCCCAGCACACTACACAGAAGTGAAGCCTTTGGAGGAGCTCACCAATAAACAAAACCCCGCAAAGTATAGGCCCTATAGCTGGGGCAAGTTTATGACTCTCAGAAAGCTCAGTAATTTCAAGAAACTCAAAGCTGAAAACATCCAAATCTATCATTTCAGGGTTTCAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

353

Amino Acids

40.71

Weight (kDa)

5.69

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 26 - 153 3.6e-33 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 199 - 298 8.7e-35 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19000 AT3G19000
fragaria_vesca FvH4_2g07071 FvH4_2g07080 FvH4_2g07080 FvH4_2g07081 FvH4_2g07082 FvH4_2g07082
malus_domestica MD05G1074000.v1.1 MD10G1088100.v1.1 MD10G1088200.v1.1 MD14G1207600.v1.1
prunus_persica Prupe.8G114100_v2.0.a1 Prupe.8G114200_v2.0.a1 Prupe.8G114300_v2.0.a1 Prupe.8G114500_v2.0.a1 Prupe.8G114800_v2.0.a1 Prupe.8G115100_v2.0.a1
pyrus_communis pycom05g06640 pycom05g06660 pycom05g06670 pycom10g07050
rosa_chinensis RchiOBHm_Chr6g0244141 RchiOBHm_Chr6g0259511 RchiOBHm_Chr6g0259521 RchiOBHm_Chr6g0259531 RchiOBHm_Chr6g0259551 RchiOBHm_Chr6g0259561
rosa_laevigata RLG00000014522 RLG00000014523 RLG00000014524 RLG00000014525 RLG00000014526 RLG00000014527
rosa_multiflora Rmu_ssc0000050.1_g000019 Rmu_ssc0000050.1_g000020 Rmu_ssc0000050.1_g000026 Rmu_ssc0000050.1_g000027 Rmu_ssc0000050.1_g000028
rosa_roxburghii Rroxscaffold_7G00206780 Rroxscaffold_7G00206790 Rroxscaffold_7G00206850 Rroxscaffold_7G00206860 Rroxscaffold_7G00206870 Rroxscaffold_7G00206880
rosa_rugosa Rorug05G0589100 Rorug05G0589200.1 Rorug05G0589300 Rorug05G0589400 Rorug05G0589500 Rorug05G0589600
rosa_samantha Rh4CG096000 Rh5BG231700 Rh6AG104900 Rh6AG105000 Rh6AG105100 Rh6AG105500 Rh6AG105600 Rh6BG098500 Rh6BG098700 Rh6BG098800 Rh6BG099000 Rh6BG099100 Rh6CG094200 Rh6CG094300 Rh6CG094400 Rh6CG094500 Rh6CG094600 Rh6DG088300 Rh6DG088400 Rh6DG088500 Rh6DG088600 Rh6DG088700
rosa_wichuraiana Rw0G014270 Rw0G014280 Rw0G014290 Rw6G009080 Rw6G009090 Rw6G009100 Rw6G009110 Rw6G009120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 674
AccB7I CCANNNNNTGG 1 cut(s) 268
AciI CCGC 3 cut(s) 242, 830, 948
AclWI GGATC 1 cut(s) 8
AcoI YGGCCR 1 cut(s) 243
AcuI CTGAAG 2 cut(s) 90, 782
AdeI CACNNNGTG 1 cut(s) 650
AfaI GTAC 1 cut(s) 687
AfiI CCNNNNNNNGG 3 cut(s) 268, 379, 969
AflIII ACRYGT 1 cut(s) 304
AgsI TTSAA 5 cut(s) 140, 485, 571, 583, 1009
AjnI CCWGG 1 cut(s) 245
AleI CACNNNNGTG 1 cut(s) 908
AluBI AGCT 8 cut(s) 20, 105, 223, 511, 928, 969, 997, 1022
AluI AGCT 8 cut(s) 20, 105, 223, 511, 928, 969, 997, 1022
Alw21I GWGCWC 1 cut(s) 930
Alw26I GTCTC 3 cut(s) 230, 342, 422
AlwI GGATC 1 cut(s) 8
AoxI GGCC 3 cut(s) 243, 449, 959
AspA2I CCTAGG 1 cut(s) 547
AspS9I GGNCC 2 cut(s) 656, 960
AsuHPI GGTGA 4 cut(s) 730, 800, 853, 922
AvaII GGWCC 1 cut(s) 656
AvrII CCTAGG 1 cut(s) 547
BanI GGYRCC 1 cut(s) 674
BanII GRGCYC 1 cut(s) 930
BbsI GAAGAC 1 cut(s) 567
Bbv12I GWGCWC 1 cut(s) 930
BccI CCATC 3 cut(s) 520, 728, 838
BciT130I CCWGG 1 cut(s) 247
BclI TGATCA 1 cut(s) 195
BcoDI GTCTC 3 cut(s) 230, 342, 422
BfaI CTAG 1 cut(s) 548
BfmI CTRYAG 1 cut(s) 964
BisI GCNGC 1 cut(s) 243
BlnI CCTAGG 1 cut(s) 547
BlsI GCNGC 1 cut(s) 244
Bme1390I CCNGG 1 cut(s) 247
Bme18I GGWCC 1 cut(s) 656
BmgT120I GGNCC 2 cut(s) 656, 960
BmiI GGNNCC 3 cut(s) 384, 658, 676
BmrFI CCNGG 1 cut(s) 247
BmrI ACTGGG 1 cut(s) 187
BmsI GCATC 1 cut(s) 658
BmuI ACTGGG 1 cut(s) 187
BpiI GAAGAC 1 cut(s) 567
BpuEI CTTGAG 3 cut(s) 209, 480, 678
BsaAI YACGTR 1 cut(s) 305
BsaJI CCNNGG 2 cut(s) 202, 547
BsaXI ACNNNNNCTCC 2 cut(s) 419, 449
Bsc4I CCNNNNNNNGG 3 cut(s) 268, 379, 969
Bse118I RCCGGY 1 cut(s) 671
Bse1I ACTGG 4 cut(s) 182, 356, 594, 693
Bse3DI GCAATG 1 cut(s) 169
BseBI CCWGG 1 cut(s) 247
BseDI CCNNGG 2 cut(s) 202, 547
BseGI GGATG 4 cut(s) 24, 301, 673, 1029
BseLI CCNNNNNNNGG 3 cut(s) 268, 379, 969
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 6 cut(s) 318, 399, 444, 650, 1003, 1012
BseNI ACTGG 4 cut(s) 182, 356, 594, 693
BseRI GAGGAG 4 cut(s) 287, 391, 395, 938
BseYI CCCAGC 3 cut(s) 261, 892, 969
BshFI GGCC 3 cut(s) 245, 451, 961
BshNI GGYRCC 1 cut(s) 674
BsiHKAI GWGCWC 1 cut(s) 930
BsiSI CCGG 1 cut(s) 672
BslFI GGGAC 2 cut(s) 543, 642
BslI CCNNNNNNNGG 3 cut(s) 268, 379, 969
BsmAI GTCTC 3 cut(s) 230, 342, 422
BsmFI GGGAC 2 cut(s) 543, 642
BsnI GGCC 3 cut(s) 245, 451, 961
Bsp1286I GDGCHC 1 cut(s) 930
Bsp143I GATC 2 cut(s) 13, 195
Bsp19I CCATGG 1 cut(s) 202
BspACI CCGC 3 cut(s) 242, 830, 948
BspANI GGCC 3 cut(s) 245, 451, 961
BspCNI CTCAG 6 cut(s) 319, 400, 445, 649, 1002, 1011
BspLI GGNNCC 3 cut(s) 384, 658, 676
BspPI GGATC 1 cut(s) 8
BspT107I GGYRCC 1 cut(s) 674
BsrDI GCAATG 1 cut(s) 169
BsrFI RCCGGY 1 cut(s) 671
BsrI ACTGG 4 cut(s) 182, 356, 594, 693
BssAI RCCGGY 1 cut(s) 671
BssECI CCNNGG 2 cut(s) 202, 547
BssMI GATC 2 cut(s) 13, 195
BssT1I CCWWGG 2 cut(s) 202, 547
Bst2UI CCWGG 1 cut(s) 247
Bst4CI ACNGT 2 cut(s) 435, 685
Bst6I CTCTTC 2 cut(s) 272, 893
BstAPI GCANNNNNTGC 1 cut(s) 168
BstBAI YACGTR 1 cut(s) 305
BstC8I GCNNGC 2 cut(s) 169, 554
BstDEI CTNAG 6 cut(s) 327, 408, 453, 636, 989, 998
BstDSI CCRYGG 1 cut(s) 202
BstENI CCTNNNNNAGG 1 cut(s) 377
BstF5I GGATG 4 cut(s) 24, 301, 673, 1029
BstKTI GATC 2 cut(s) 16, 198
BstMAI GTCTC 3 cut(s) 230, 342, 422
BstMBI GATC 2 cut(s) 13, 195
BstMWI GCNNNNNNNGC 1 cut(s) 168
BstNI CCWGG 1 cut(s) 247
BstNSI RCATGY 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 245
BstSFI CTRYAG 1 cut(s) 964
BstV2I GAAGAC 1 cut(s) 567
BsuRI GGCC 3 cut(s) 245, 451, 961
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 4 cut(s) 24, 301, 673, 1029
BtsIMutI CAGTG 2 cut(s) 324, 440
Cac8I GCNNGC 2 cut(s) 169, 554
Cfr10I RCCGGY 1 cut(s) 671
Cfr13I GGNCC 2 cut(s) 656, 960
Csp6I GTAC 1 cut(s) 686
CviAII CATG 2 cut(s) 168, 203
CviQI GTAC 1 cut(s) 686
DdeI CTNAG 6 cut(s) 327, 408, 453, 636, 989, 998
DpnI GATC 2 cut(s) 15, 197
DpnII GATC 2 cut(s) 13, 195
DraIII CACNNNGTG 1 cut(s) 650
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 2 cut(s) 272, 893
EarI CTCTTC 2 cut(s) 272, 893
Ecl136II GAGCTC 1 cut(s) 928
Eco130I CCWWGG 2 cut(s) 202, 547
Eco24I GRGCYC 1 cut(s) 930
Eco47I GGWCC 1 cut(s) 656
Eco53kI GAGCTC 1 cut(s) 928
Eco57I CTGAAG 2 cut(s) 90, 782
EcoICRI GAGCTC 1 cut(s) 928
EcoNI CCTNNNNNAGG 1 cut(s) 377
EcoO109I RGGNCCY 1 cut(s) 960
EcoRII CCWGG 1 cut(s) 245
EcoT14I CCWWGG 2 cut(s) 202, 547
EcoT22I ATGCAT 1 cut(s) 169
EcoT38I GRGCYC 1 cut(s) 930
ErhI CCWWGG 2 cut(s) 202, 547
FaeI CATG 2 cut(s) 171, 206
FaqI GGGAC 2 cut(s) 543, 642
FatI CATG 2 cut(s) 167, 202
FauI CCCGC 1 cut(s) 955
FbaI TGATCA 1 cut(s) 195
Fnu4HI GCNGC 1 cut(s) 243
FokI GGATG 4 cut(s) 11, 308, 680, 1016
FriOI GRGCYC 1 cut(s) 930
Fsp4HI GCNGC 1 cut(s) 243
FspBI CTAG 1 cut(s) 548
GluI GCNGC 1 cut(s) 243
GsaI CCCAGC 3 cut(s) 265, 896, 973
HaeIII GGCC 3 cut(s) 245, 451, 961
HapII CCGG 1 cut(s) 672
Hin1II CATG 2 cut(s) 171, 206
HinfI GANTC 4 cut(s) 113, 606, 745, 985
HpaII CCGG 1 cut(s) 672
HphI GGTGA 4 cut(s) 730, 800, 853, 922
Hpy166II GTNNAC 1 cut(s) 850
Hpy188I TCNGA 5 cut(s) 124, 605, 755, 992, 1057
Hpy188III TCNNGA 5 cut(s) 226, 464, 497, 695, 1009
Hpy8I GTNNAC 1 cut(s) 850
HpyAV CCTTC 7 cut(s) 65, 223, 349, 639, 853, 889, 893
HpyCH4III ACNGT 2 cut(s) 435, 685
HpyCH4IV ACGT 1 cut(s) 304
HpyCH4V TGCA 2 cut(s) 167, 171
HpyF10VI GCNNNNNNNGC 1 cut(s) 168
HpyF3I CTNAG 6 cut(s) 327, 408, 453, 636, 989, 998
HpySE526I ACGT 1 cut(s) 304
Hsp92II CATG 2 cut(s) 171, 206
Ksp22I TGATCA 1 cut(s) 195
Kzo9I GATC 2 cut(s) 13, 195
LmnI GCTCC 4 cut(s) 110, 382, 807, 925
LweI GCATC 1 cut(s) 658
MaeI CTAG 1 cut(s) 548
MaeII ACGT 1 cut(s) 304
MaeIII GTNAC 2 cut(s) 314, 788
MalI GATC 2 cut(s) 15, 197
MboI GATC 2 cut(s) 13, 195
MboII GAAGA 7 cut(s) 178, 289, 295, 497, 572, 788, 880
MhlI GDGCHC 1 cut(s) 930
MluCI AATT 4 cut(s) 467, 534, 610, 1003
MlyI GAGTC 3 cut(s) 107, 600, 979
MmeI TCCRAC 1 cut(s) 685
Mph1103I ATGCAT 1 cut(s) 169
MseI TTAA 5 cut(s) 101, 441, 470, 680, 1060
MslI CAYNNNNRTG 3 cut(s) 140, 666, 908
MspI CCGG 1 cut(s) 672
MspR9I CCNGG 1 cut(s) 247
MvaI CCWGG 1 cut(s) 247
MwoI GCNNNNNNNGC 1 cut(s) 168
NcoI CCATGG 1 cut(s) 202
NdeII GATC 2 cut(s) 13, 195
NlaIII CATG 2 cut(s) 171, 206
NlaIV GGNNCC 3 cut(s) 384, 658, 676
NmuCI GTSAC 1 cut(s) 788
NsiI ATGCAT 1 cut(s) 169
NspI RCATGY 1 cut(s) 171
OliI CACNNNNGTG 1 cut(s) 908
PaeI GCATGC 1 cut(s) 171
PfeI GAWTC 1 cut(s) 745
PflMI CCANNNNNTGG 1 cut(s) 268
PkrI GCNGC 1 cut(s) 244
PleI GAGTC 3 cut(s) 107, 600, 979
PpsI GAGTC 3 cut(s) 107, 600, 979
Ppu21I YACGTR 1 cut(s) 305
Psp124BI GAGCTC 1 cut(s) 930
Psp6I CCWGG 1 cut(s) 245
PspFI CCCAGC 3 cut(s) 261, 892, 969
PspGI CCWGG 1 cut(s) 245
PspN4I GGNNCC 3 cut(s) 384, 658, 676
PspPI GGNCC 2 cut(s) 656, 960
RsaI GTAC 1 cut(s) 687
RsaNI GTAC 1 cut(s) 686
RseI CAYNNNNRTG 3 cut(s) 140, 666, 908
SacI GAGCTC 1 cut(s) 930
SaqAI TTAA 5 cut(s) 101, 441, 470, 680, 1060
SatI GCNGC 1 cut(s) 243
Sau3AI GATC 2 cut(s) 13, 195
Sau96I GGNCC 2 cut(s) 656, 960
SchI GAGTC 3 cut(s) 107, 600, 979
ScrFI CCNGG 1 cut(s) 247
SduI GDGCHC 1 cut(s) 930
SfaNI GCATC 1 cut(s) 658
SfcI CTRYAG 1 cut(s) 964
SinI GGWCC 1 cut(s) 656
SmiMI CAYNNNNRTG 3 cut(s) 140, 666, 908
SmlI CTYRAG 3 cut(s) 224, 495, 693
SmoI CTYRAG 3 cut(s) 224, 495, 693
SphI GCATGC 1 cut(s) 171
Sse9I AATT 4 cut(s) 467, 534, 610, 1003
SsiI CCGC 3 cut(s) 242, 830, 948
SspI AATATT 1 cut(s) 491
SspMI CTAG 1 cut(s) 548
SstI GAGCTC 1 cut(s) 930
StyD4I CCNGG 1 cut(s) 245
StyI CCWWGG 2 cut(s) 202, 547
TaaI ACNGT 2 cut(s) 435, 685
TaiI ACGT 1 cut(s) 307
TaqI TCGA 2 cut(s) 63, 404
TasI AATT 4 cut(s) 467, 534, 610, 1003
TatI WGTACW 1 cut(s) 685
TauI GCSGC 1 cut(s) 245
TfiI GAWTC 1 cut(s) 745
Tru1I TTAA 5 cut(s) 101, 441, 470, 680, 1060
Tru9I TTAA 5 cut(s) 101, 441, 470, 680, 1060
TscAI CASTG 2 cut(s) 331, 440
TseFI GTSAC 1 cut(s) 788
Tsp45I GTSAC 1 cut(s) 788
TspDTI ATGAA 2 cut(s) 13, 312
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 2 cut(s) 331, 440
Van91I CCANNNNNTGG 1 cut(s) 268
VpaK11BI GGWCC 1 cut(s) 656
XagI CCTNNNNNAGG 1 cut(s) 377
XceI RCATGY 1 cut(s) 171
XmaJI CCTAGG 1 cut(s) 547
XspI CTAG 1 cut(s) 548
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.