Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000720

Physical Location & Seq

Genomic Coordinates Reverse (-)

39487004 .. 39496405

Gene Structure 9402 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1310200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1392 bp

ATGGCACACTTCCTTCAAGCTCACTCTGCTTCCTCCAAGGGACAAGTTATTGGCGACCTTCAGGTTCGCTATGCAAAGAAATTGAAGGAATTGAAGGATGCACTCGCAAACATCGGAGAAGATGAACAATTGATTGCGGTTGACGCCATGCAACGCCTAGGCCAATGCACGAGAGCTTATGGTGATGAAGGCAGTGATGAACTTGGTGTGGTTTCACTTCGTTTTCGACTACTGAGACAACAAGGTTATCATGTAACCACAGATGCAATATTTAATAAGTTCAAGAACAACAAGGAAGTCGACATAAGCGGATTAGTAGGATTATATGAAGCATCTCATTTGAGCTTCCCAGGAGAAGAGTCACTTGATGAAGCTGGAAGGCGAAGCCACCAGCTTCTCACGGATTGGATGAAAAACAACCTCGATGATCATCAAGCTTCATCCTTCGCGTACTCATTAGAGCATCCTTATCACAAGAGCTTGACAAGATTCATGGCTAAGAACTACTTAAATAATTTCGAAGGGAAGGAGAAATGGGTCAATGTTTTGCGGGAGCTAGCAAAACTAGAGTTCAACATGGTTGAATCTCTAATCCGAAATGAAGTTCAACAAGTATCCAAGTGGTGGAAAGAACTAGGCTTGACTAAGGAGTTGAAGTTCGTGAGAGACCAGCCAATTAAATGGTATACGTGGCCGATGGCATGCCTAGCAGATCCAAGCTTATCAGAGGAAAGGGTTGAACTCACAAAATCGATCTCTCTTGTCTACATAATTGACGATATTTTTGATGTTCAGGGCACCCTCGATGAACTCATTCTCTTCACAGCTGCAGTTGAGAGGCACTTGCCAAAAAGTGAAGAGTACTTGAACAACGGGTTCATCAGTTCAGGGGTGCATGTGGTTCTAACACATGCATTCTTCTTACTGGGTCAAGGTATAAGCAAGGAAACTGTACGTATTCTGGACAACATCAACATATCTAGCATTATATCTTCAACCGAAAAAATTCTTCGGCTATGGGATGACTCTGGAAGTGCCAAGTTTCGTACTTGTATTTTGATATGGTTAGATGAGGACCAAGATGGAAATGATAGTTCATACATAGAGTGTTACATGAATGAAAATCAAGGTTGTTCAGAGGAAGATGCACGAGCATTCGTTGTCCATAGGATTTCGGAAGAATGGAAGTTCCTCAACCAAGAATGTTTGTCTGCATCAAATCCATTTTCAGCATCCTTTATAAAACTTGCTCTTAATGTTGCTAGAATGGTCCCATTGATGTATGATTATAATTCTCAACATAGCCTTCCAAGCCTTGTGGAGAATATGAAGTCACTGCTTTCTGATAGTTTTCTTGCACAAGACTTAAGTAACCAGCGCAGCCAAGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006720 GO:0006721 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009987 GO:0010333 GO:0010334 GO:0016101 GO:0016102 GO:0016114 GO:0016829 GO:0016835 GO:0016838 GO:0031347 GO:0034007 GO:0042214 GO:0042742 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043692 GO:0043693 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046246 GO:0046872 GO:0048583 GO:0050789 GO:0050896 GO:0051704 GO:0051707 GO:0051761 GO:0051762 GO:0065007 GO:0071704 GO:0080013 GO:0080134 GO:0098542 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00902 ko00909 ko01100 ko01110 ko01130 map00902 map00909 map01100 map01110 map01130

Pfam Domains

Protein Families

Terpene_synth Terpene_synth_C

Protein Analysis

464

Amino Acids

52.83

Weight (kDa)

5.24

Isoelectric Point (pI)

42.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Terpene_synth	PF01397	60 - 144	1.2e-19	Terpene synthase, N-terminal domain
Terpene_synth_C	PF03936	210 - 280	1.3e-20	Terpene synthase family, metal binding domain
Terpene_synth_C	PF03936	280 - 396	1.3e-19	Terpene synthase family, metal binding domain
Terpene_syn_C_2	PF19086	280 - 396	3.6e-18	Terpene synthase family 2, C-terminal metal binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0000720)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61680 AT1G61680
fragaria_vesca	FvH4_3g03042 FvH4_3g03042 FvH4_3g03045 FvH4_3g03061 FvH4_3g03072 FvH4_3g03072 FvH4_3g03081 FvH4_3g03150 FvH4_3g03150
malus_domestica	MD05G1334800.v1.1 MD05G1335000.v1.1 MD05G1335100.v1.1 MD10G1309700.v1.1 MD10G1309800.v1.1 MD10G1309900.v1.1 MD10G1310200.v1.1 MD10G1310300.v1.1 MD10G1310500.v1.1 MD10G1310600.v1.1
prunus_persica	Prupe.4G030300_v2.0.a1 Prupe.4G030400_v2.0.a1
pyrus_communis	pycom05g30640 pycom10g26110 pycom10g26140 pycom10g26150 pycom10g26160
rosa_chinensis	RchiOBHm_Chr5g0004711 RchiOBHm_Chr5g0004731 RchiOBHm_Chr5g0004761 RchiOBHm_Chr5g0004801
rosa_laevigata	RLG00000030230 RLG00000031254 RLG00000031255 RLG00000031257 RLG00000031260
rosa_multiflora	Rmu_co8094102.1_g000001 Rmu_sc0002210.1_g000005 Rmu_sc0002210.1_g000008 Rmu_sc0002210.1_g000009 Rmu_sc0008308.1_g000013 Rmu_sc0010871.1_g000028 Rmu_sc0030338.1_g000001
rosa_roxburghii	Rroxscaffold_1G00070820 Rroxscaffold_1G00070840 Rroxscaffold_1G00070850
rosa_rugosa	Rorug04G0413500 Rorug04G0413500 Rorug04G0413700 Rorug04G0413800 Rorug04G0413900 Rorug05G0165300
rosa_samantha	Rh5AG041200 Rh5AG041300 Rh5BG040900 Rh5BG041000 Rh5BG041100 Rh5BG041300 Rh5CG044700 Rh5CG044800 Rh5CG045200 Rh5DG040900 Rh5DG041000 Rh5DG041200 Rh5DG041700 Rh5DG265100
rosa_wichuraiana	Rw5G003960 Rw5G003980 Rw5G003990 Rw5G004000 Rw5G004010 Rw5G004040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1241, 1290
AccB1I	GGYRCC	1 cut(s)	797
AccB7I	CCANNNNNTGG	1 cut(s)	624
AccI	GTMKAC	3 cut(s)	300, 686, 765
AccII	CGCG	1 cut(s)	449
AciI	CCGC	3 cut(s)	137, 309, 550
AclWI	GGATC	1 cut(s)	707
AcoI	YGGCCR	1 cut(s)	692
AcsI	RAATTY	1 cut(s)	1005
AcuI	CTGAAG	1 cut(s)	44
AcyI	GRCGYC	1 cut(s)	144
AfaI	GTAC	4 cut(s)	452, 863, 954, 1048
AfiI	CCNNNNNNNGG	1 cut(s)	624
AflII	CTTAAG	1 cut(s)	1366
AjnI	CCWGG	1 cut(s)	349
AloI	GAACNNNNNNTCC	2 cut(s)	641, 673
Alw26I	GTCTC	2 cut(s)	229, 660
AlwI	GGATC	1 cut(s)	707
AoxI	GGCC	2 cut(s)	160, 692
ApeKI	GCWGC	2 cut(s)	827, 1380
ApoI	RAATTY	1 cut(s)	1005
ArsI	GACNNNNNNTTYG	2 cut(s)	767, 799
Asp700I	GAANNNNTTC	2 cut(s)	813, 1005
AspA2I	CCTAGG	1 cut(s)	157
AspLEI	GCGC	1 cut(s)	1380
AspS9I	GGNCC	2 cut(s)	1075, 1270
AsuHPI	GGTGA	1 cut(s)	194
AsuII	TTCGAA	1 cut(s)	519
AsuNHI	GCTAGC	1 cut(s)	556
AvaII	GGWCC	2 cut(s)	1075, 1270
AvrII	CCTAGG	1 cut(s)	157
BaeGI	GKGCMC	1 cut(s)	800
BanI	GGYRCC	1 cut(s)	797
BauI	CACGAG	2 cut(s)	169, 1149
BbvI	GCAGC	1 cut(s)	814
BccI	CCATC	2 cut(s)	691, 1076
BciT130I	CCWGG	1 cut(s)	351
BciVI	GTATCC	1 cut(s)	625
BclI	TGATCA	1 cut(s)	427
BcoDI	GTCTC	2 cut(s)	229, 660
BfaI	CTAG	7 cut(s)	158, 557, 566, 635, 707, 981, 1263
BfmI	CTRYAG	1 cut(s)	828
BfrI	CTTAAG	1 cut(s)	1366
BfuI	GTATCC	1 cut(s)	625
BisI	GCNGC	2 cut(s)	828, 1381
BlnI	CCTAGG	1 cut(s)	157
BlsI	GCNGC	2 cut(s)	829, 1382
BmcAI	AGTACT	1 cut(s)	863
Bme1390I	CCNGG	1 cut(s)	351
Bme18I	GGWCC	2 cut(s)	1075, 1270
BmgT120I	GGNCC	2 cut(s)	1075, 1270
BmiI	GGNNCC	2 cut(s)	799, 1272
BmrFI	CCNGG	1 cut(s)	351
BmrI	ACTGGG	1 cut(s)	935
BmsI	GCATC	7 cut(s)	88, 253, 341, 472, 1135, 1223, 1241
BmtI	GCTAGC	1 cut(s)	560
BmuI	ACTGGG	1 cut(s)	935
Bpu14I	TTCGAA	1 cut(s)	519
Bsa29I	ATCGAT	1 cut(s)	752
BsaAI	YACGTR	2 cut(s)	690, 956
BsaBI	GATNNNNATC	1 cut(s)	429
BsaHI	GRCGYC	1 cut(s)	144
BsaI	GGTCTC	1 cut(s)	660
BsaJI	CCNNGG	3 cut(s)	36, 157, 349
BsaXI	ACNNNNNCTCC	2 cut(s)	641, 671
Bsc4I	CCNNNNNNNGG	1 cut(s)	624
Bse1I	ACTGG	1 cut(s)	930
Bse8I	GATNNNNATC	1 cut(s)	429
BseBI	CCWGG	1 cut(s)	351
BseCI	ATCGAT	1 cut(s)	752
BseDI	CCNNGG	3 cut(s)	36, 157, 349
BseGI	GGATG	6 cut(s)	103, 414, 440, 463, 1027, 1232
BseJI	GATNNNNATC	1 cut(s)	429
BseLI	CCNNNNNNNGG	1 cut(s)	624
BseMII	CTCAG	1 cut(s)	224
BseNI	ACTGG	1 cut(s)	930
BseSI	GKGCMC	1 cut(s)	800
BseXI	GCAGC	1 cut(s)	814
Bsh1236I	CGCG	1 cut(s)	449
BshFI	GGCC	2 cut(s)	162, 694
BshNI	GGYRCC	1 cut(s)	797
BshVI	ATCGAT	1 cut(s)	752
BslFI	GGGAC	2 cut(s)	54, 1256
BslI	CCNNNNNNNGG	1 cut(s)	624
BsmAI	GTCTC	2 cut(s)	229, 660
BsmFI	GGGAC	2 cut(s)	54, 1256
BsmI	GAATGC	2 cut(s)	914, 1154
BsnI	GGCC	2 cut(s)	162, 694
Bso31I	GGTCTC	1 cut(s)	660
Bsp119I	TTCGAA	1 cut(s)	519
Bsp1286I	GDGCHC	1 cut(s)	800
Bsp143I	GATC	3 cut(s)	427, 712, 753
BspACI	CCGC	3 cut(s)	137, 309, 550
BspANI	GGCC	2 cut(s)	162, 694
BspCNI	CTCAG	1 cut(s)	225
BspDI	ATCGAT	1 cut(s)	752
BspFNI	CGCG	1 cut(s)	449
BspLI	GGNNCC	2 cut(s)	799, 1272
BspMAI	CTGCAG	1 cut(s)	832
BspOI	GCTAGC	1 cut(s)	560
BspPI	GGATC	1 cut(s)	707
BspT104I	TTCGAA	1 cut(s)	519
BspT107I	GGYRCC	1 cut(s)	797
BspTI	CTTAAG	1 cut(s)	1366
BspTNI	GGTCTC	1 cut(s)	660
BsrI	ACTGG	1 cut(s)	930
BssECI	CCNNGG	3 cut(s)	36, 157, 349
BssMI	GATC	3 cut(s)	427, 712, 753
BssNAI	GTATAC	1 cut(s)	687
BssNI	GRCGYC	1 cut(s)	144
BssSI	CACGAG	2 cut(s)	169, 1149
BssT1I	CCWWGG	2 cut(s)	36, 157
Bst1107I	GTATAC	1 cut(s)	687
Bst2BI	CACGAG	2 cut(s)	169, 1149
Bst2UI	CCWGG	1 cut(s)	351
Bst4CI	ACNGT	1 cut(s)	952
Bst6I	CTCTTC	3 cut(s)	351, 824, 852
BstACI	GRCGYC	1 cut(s)	144
BstAFI	CTTAAG	1 cut(s)	1366
BstBAI	YACGTR	2 cut(s)	690, 956
BstBI	TTCGAA	1 cut(s)	519
BstC8I	GCNNGC	2 cut(s)	558, 703
BstDEI	CTNAG	3 cut(s)	233, 498, 645
BstF5I	GGATG	6 cut(s)	103, 414, 440, 463, 1027, 1232
BstFNI	CGCG	1 cut(s)	449
BstHHI	GCGC	1 cut(s)	1380
BstKTI	GATC	3 cut(s)	430, 715, 756
BstMAI	GTCTC	2 cut(s)	229, 660
BstMBI	GATC	3 cut(s)	427, 712, 753
BstMWI	GCNNNNNNNGC	4 cut(s)	26, 143, 707, 1311
BstNI	CCWGG	1 cut(s)	351
BstNSI	RCATGY	3 cut(s)	705, 899, 914
BstSCI	CCNGG	1 cut(s)	349
BstSFI	CTRYAG	1 cut(s)	828
BstSLI	GKGCMC	1 cut(s)	800
BstSNI	TACGTA	1 cut(s)	956
BstUI	CGCG	1 cut(s)	449
BstV1I	GCAGC	1 cut(s)	814
BstX2I	RGATCY	1 cut(s)	712
BstXI	CCANNNNNNTGG	1 cut(s)	681
BstYI	RGATCY	1 cut(s)	712
BstZ17I	GTATAC	1 cut(s)	687
Bsu15I	ATCGAT	1 cut(s)	752
BsuI	GTATCC	1 cut(s)	625
BsuRI	GGCC	2 cut(s)	162, 694
BsuTUI	ATCGAT	1 cut(s)	752
BtsCI	GGATG	6 cut(s)	103, 414, 440, 463, 1027, 1232
BtsI	GCAGTG	2 cut(s)	199, 1334
BtsIMutI	CAGTG	2 cut(s)	199, 1334
Cac8I	GCNNGC	2 cut(s)	558, 703
CfoI	GCGC	1 cut(s)	1380
Cfr13I	GGNCC	2 cut(s)	1075, 1270
ClaI	ATCGAT	1 cut(s)	752
CseI	GACGC	1 cut(s)	152
Csp6I	GTAC	4 cut(s)	451, 862, 953, 1047
CspCI	CAANNNNNGTGG	2 cut(s)	1299, 1334
CviAII	CATG	8 cut(s)	148, 251, 493, 577, 702, 896, 911, 1114
CviQI	GTAC	4 cut(s)	451, 862, 953, 1047
DdeI	CTNAG	3 cut(s)	233, 498, 645
DpnI	GATC	3 cut(s)	429, 714, 755
DpnII	GATC	3 cut(s)	427, 712, 753
EaeI	YGGCCR	1 cut(s)	692
Eam1104I	CTCTTC	3 cut(s)	351, 824, 852
EarI	CTCTTC	3 cut(s)	351, 824, 852
Eco105I	TACGTA	1 cut(s)	956
Eco130I	CCWWGG	2 cut(s)	36, 157
Eco31I	GGTCTC	1 cut(s)	660
Eco47I	GGWCC	2 cut(s)	1075, 1270
Eco57I	CTGAAG	1 cut(s)	44
EcoRII	CCWGG	1 cut(s)	349
EcoT14I	CCWWGG	2 cut(s)	36, 157
EcoT22I	ATGCAT	1 cut(s)	916
ErhI	CCWWGG	2 cut(s)	36, 157
FaeI	CATG	8 cut(s)	151, 254, 496, 580, 705, 899, 914, 1117
FalI	AAGNNNNNCTT	4 cut(s)	348, 380, 491, 523
FaqI	GGGAC	2 cut(s)	54, 1256
FatI	CATG	8 cut(s)	147, 250, 492, 576, 701, 895, 910, 1113
FauI	CCCGC	1 cut(s)	543
FbaI	TGATCA	1 cut(s)	427
FblI	GTMKAC	3 cut(s)	300, 686, 765
Fnu4HI	GCNGC	2 cut(s)	828, 1381
FokI	GGATG	6 cut(s)	110, 421, 427, 450, 1034, 1219
Fsp4HI	GCNGC	2 cut(s)	828, 1381
FspBI	CTAG	7 cut(s)	158, 557, 566, 635, 707, 981, 1263
GlaI	GCGC	1 cut(s)	1379
GluI	GCNGC	2 cut(s)	828, 1381
HaeIII	GGCC	2 cut(s)	162, 694
HgaI	GACGC	1 cut(s)	152
HhaI	GCGC	1 cut(s)	1380
Hin1I	GRCGYC	1 cut(s)	144
Hin1II	CATG	8 cut(s)	151, 254, 496, 580, 705, 899, 914, 1117
Hin6I	GCGC	1 cut(s)	1378
HinP1I	GCGC	1 cut(s)	1378
HincII	GTYRAC	2 cut(s)	142, 301
HindII	GTYRAC	2 cut(s)	142, 301
HindIII	AAGCTT	2 cut(s)	435, 718
HinfI	GANTC	4 cut(s)	359, 489, 584, 1025
HphI	GGTGA	1 cut(s)	194
Hpy166II	GTNNAC	4 cut(s)	142, 301, 687, 766
Hpy188I	TCNGA	6 cut(s)	116, 596, 727, 1138, 1177, 1345
Hpy188III	TCNNGA	4 cut(s)	283, 661, 962, 1029
Hpy8I	GTNNAC	4 cut(s)	142, 301, 687, 766
HpyCH4III	ACNGT	1 cut(s)	952
HpyCH4IV	ACGT	2 cut(s)	689, 955
HpyF10VI	GCNNNNNNNGC	4 cut(s)	26, 143, 707, 1311
HpyF3I	CTNAG	3 cut(s)	233, 498, 645
HpySE526I	ACGT	2 cut(s)	689, 955
Hsp92I	GRCGYC	1 cut(s)	144
Hsp92II	CATG	8 cut(s)	151, 254, 496, 580, 705, 899, 914, 1117
HspAI	GCGC	1 cut(s)	1378
Ksp22I	TGATCA	1 cut(s)	427
Kzo9I	GATC	3 cut(s)	427, 712, 753
LmnI	GCTCC	1 cut(s)	553
Lsp1109I	GCAGC	1 cut(s)	814
LweI	GCATC	7 cut(s)	88, 253, 341, 472, 1135, 1223, 1241
MaeI	CTAG	7 cut(s)	158, 557, 566, 635, 707, 981, 1263
MaeII	ACGT	2 cut(s)	689, 955
MaeIII	GTNAC	5 cut(s)	253, 360, 1109, 1332, 1370
MalI	GATC	3 cut(s)	429, 714, 755
MboI	GATC	3 cut(s)	427, 712, 753
MboII	GAAGA	9 cut(s)	131, 368, 811, 869, 910, 984, 1001, 1154, 1190
MfeI	CAATTG	1 cut(s)	128
MflI	RGATCY	1 cut(s)	712
MhlI	GDGCHC	1 cut(s)	800
MluCI	AATT	8 cut(s)	80, 89, 128, 514, 675, 771, 1005, 1291
MlyI	GAGTC	2 cut(s)	368, 1019
MnlI	CCTC	8 cut(s)	43, 431, 721, 812, 831, 1066, 1132, 1202
Mph1103I	ATGCAT	1 cut(s)	916
MroXI	GAANNNNTTC	2 cut(s)	813, 1005
MseI	TTAA	5 cut(s)	273, 509, 678, 1254, 1367
MspA1I	CMGCKG	1 cut(s)	827
MspCI	CTTAAG	1 cut(s)	1366
MspR9I	CCNGG	1 cut(s)	351
MunI	CAATTG	1 cut(s)	128
Mva1269I	GAATGC	2 cut(s)	914, 1154
MvaI	CCWGG	1 cut(s)	351
MvnI	CGCG	1 cut(s)	449
MwoI	GCNNNNNNNGC	4 cut(s)	26, 143, 707, 1311
NdeII	GATC	3 cut(s)	427, 712, 753
NheI	GCTAGC	1 cut(s)	556
NlaIII	CATG	8 cut(s)	151, 254, 496, 580, 705, 899, 914, 1117
NlaIV	GGNNCC	2 cut(s)	799, 1272
NmuCI	GTSAC	2 cut(s)	360, 1332
NsiI	ATGCAT	1 cut(s)	916
NspI	RCATGY	3 cut(s)	705, 899, 914
NspV	TTCGAA	1 cut(s)	519
PaeI	GCATGC	1 cut(s)	705
PctI	GAATGC	2 cut(s)	914, 1154
PdmI	GAANNNNTTC	2 cut(s)	813, 1005
PfeI	GAWTC	2 cut(s)	489, 584
PflMI	CCANNNNNTGG	1 cut(s)	624
PkrI	GCNGC	2 cut(s)	829, 1382
PleI	GAGTC	2 cut(s)	367, 1019
PpsI	GAGTC	2 cut(s)	367, 1019
Ppu21I	YACGTR	2 cut(s)	690, 956
PsiI	TTATAA	2 cut(s)	1241, 1290
Psp6I	CCWGG	1 cut(s)	349
PspGI	CCWGG	1 cut(s)	349
PspN4I	GGNNCC	2 cut(s)	799, 1272
PspPI	GGNCC	2 cut(s)	1075, 1270
PstI	CTGCAG	1 cut(s)	832
PsuI	RGATCY	1 cut(s)	712
PvuII	CAGCTG	1 cut(s)	827
RsaI	GTAC	4 cut(s)	452, 863, 954, 1048
RsaNI	GTAC	4 cut(s)	451, 862, 953, 1047
SalI	GTCGAC	1 cut(s)	299
SaqAI	TTAA	5 cut(s)	273, 509, 678, 1254, 1367
SatI	GCNGC	2 cut(s)	828, 1381
Sau3AI	GATC	3 cut(s)	427, 712, 753
Sau96I	GGNCC	2 cut(s)	1075, 1270
ScaI	AGTACT	1 cut(s)	863
SchI	GAGTC	2 cut(s)	368, 1019
ScrFI	CCNGG	1 cut(s)	351
SduI	GDGCHC	1 cut(s)	800
SfaNI	GCATC	7 cut(s)	88, 253, 341, 472, 1135, 1223, 1241
SfcI	CTRYAG	1 cut(s)	828
SfuI	TTCGAA	1 cut(s)	519
SinI	GGWCC	2 cut(s)	1075, 1270
SmlI	CTYRAG	1 cut(s)	1366
SmoI	CTYRAG	1 cut(s)	1366
SnaBI	TACGTA	1 cut(s)	956
SphI	GCATGC	1 cut(s)	705
Sse9I	AATT	8 cut(s)	80, 89, 128, 514, 675, 771, 1005, 1291
SsiI	CCGC	3 cut(s)	137, 309, 550
SspI	AATATT	1 cut(s)	270
SspMI	CTAG	7 cut(s)	158, 557, 566, 635, 707, 981, 1263
StyD4I	CCNGG	1 cut(s)	349
StyI	CCWWGG	2 cut(s)	36, 157
TaaI	ACNGT	1 cut(s)	952
TaiI	ACGT	2 cut(s)	692, 958
TaqI	TCGA	6 cut(s)	226, 300, 423, 519, 752, 804
TasI	AATT	8 cut(s)	80, 89, 128, 514, 675, 771, 1005, 1291
TatI	WGTACW	1 cut(s)	861
TfiI	GAWTC	2 cut(s)	489, 584
Tru1I	TTAA	5 cut(s)	273, 509, 678, 1254, 1367
Tru9I	TTAA	5 cut(s)	273, 509, 678, 1254, 1367
TscAI	CASTG	2 cut(s)	199, 1341
TseFI	GTSAC	2 cut(s)	360, 1332
TseI	GCWGC	2 cut(s)	827, 1380
Tsp45I	GTSAC	2 cut(s)	360, 1332
TspGWI	ACGGA	1 cut(s)	416
TspRI	CASTG	2 cut(s)	199, 1341
Van91I	CCANNNNNTGG	1 cut(s)	624
Vha464I	CTTAAG	1 cut(s)	1366
VpaK11BI	GGWCC	2 cut(s)	1075, 1270
XapI	RAATTY	1 cut(s)	1005
XceI	RCATGY	3 cut(s)	705, 899, 914
XmaJI	CCTAGG	1 cut(s)	157
XmiI	GTMKAC	3 cut(s)	300, 686, 765
XmnI	GAANNNNTTC	2 cut(s)	813, 1005
XspI	CTAG	7 cut(s)	158, 557, 566, 635, 707, 981, 1263
ZrmI	AGTACT	1 cut(s)	863
Zsp2I	ATGCAT	1 cut(s)	916

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.