Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000720

Physical Location & Seq

Genomic Coordinates Reverse (-)

39558786 .. 39561964

Gene Structure 3179 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1310600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1605 bp

ATGGCACACTTCCTTCAAGCTCACTCTGCTTCCTCCAAGGGACAAGTTATTGGCGACCTTCAGGTTCGCCATGCAAAGAAATTGAAGGAATTGAAGGAGGCACTCGCAAATATCGGAGAAAATGAACAATTGATTGCGGTTGACGCCATGCAACGCCTAGGCGTTGACTATCATTTCCAAGAGGAGATTGAAGCAATTTTAAAAAAGCAATGTACGAGAGCTTCTGGCGATGAAGGCAGTGATGAACTTGGTGTGGTTTCACTTCGTTTTCGACTACTGAGACAGCAAGGTTATCATGTAACCACAGATGCATTTAATAAGTTCAAGAACAACGAGGAATTCAAAGTCGACATAAGCGGACTAGTGGGATTATATGAAGCGTCTCATTTGAGTTTCCCAGGGGAAGAGACACTTGATGAAGCTGGAAGGCGAAGCCACCAGCTTCTCACAGATTGGATGAAAAACAATCTCGATGATCATCAAGCTTCAGCAGTCGCGTACTCATTAGAGCATCCTTATCACAAGAGCTTGACAAGATTCAGGGCTAAGAACTTCTTAAATAATTTCGAAGGAAAGCAGAAATGGGTCAATGTTTTGCGGGAGCTAGCAAAACTAGAGTTCAACATGGTTGAATCTCTAATCCGAAATGAAGTTCAACAAGTATCCAAGTGGTGGAAAGAACTAGGCTTGACTAAGGAGTTGAAGTTCGTGAGAGACCAGCCAATTAAATGGTATACGTGGCCGATGACATGCCTTGCAGATCCAAGCTTATCAGAGGAAAGGGTTGAACTCACAAAATCGATCTCTCTTGTCTACATAATTGACGATATTTTTGATGTTCAGGGCACCCTCGATGAACTCATTCTCTTCACAGCTGCAGTTGAGAGATGGGATATTGATGCAACTGGTGAACTACCGAATTACATGAAGATATGTTTTAAGGCTCTTTATGACATTACCAATGAAACCAGCTACATTGTGCACAAAAGGCATGGATGGAACCCTCTAGAGTCCCTTAAAAAATCGTGGGCAACATTATGCAAAGCGTTCCTACTAGAAGCGCGATGGTTTAGTTGCGGGCACTTGCCAAACAGTGAAGAGTACTTGAACAACGGGTTCATCAGTTCAGGGGTGCCTGTGGTTCTAACACATGCATTCTTCTTACTGGGTCAAGGTATAAGCAAGGAAACCGTACGTATTGTGGACAACATCAACATATCTAGCATTATATCTTCAACCGCAACAATTCTTCGGCTATGGGATGACTTTGGAAGTGCCAAGGATGAGGACCAAGATGGAAATGATGGTTCATACATAGCGTGTTACGTGAATGAAAATCAAGGTTGTTCAGACGAAGATGCACGAGCATTCGTTGTCCATAGGATTTCGGAAGAATGGAAGTTCCTCAACAAAGAATGTTTGTCTGCATCAAATCCATTTTCAGCATCTTTTACAAAACTTGCTCTTAATGTTGCTAGAATGGTCCCATTGATGTATGATTATAATTCTCAACATAGCCTTCCAAGCCTCGAGGAGAATATGAAGTCGCTGCTTTCTGATAGTTTTCTTGCGCAAGGCTTAAGCAACCAGCGCAGCCAAGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006720 GO:0006721 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009987 GO:0010333 GO:0010334 GO:0016101 GO:0016102 GO:0016114 GO:0016829 GO:0016835 GO:0016838 GO:0031347 GO:0034007 GO:0042214 GO:0042742 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043692 GO:0043693 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046246 GO:0046872 GO:0048583 GO:0050789 GO:0050896 GO:0051704 GO:0051707 GO:0051761 GO:0051762 GO:0065007 GO:0071704 GO:0080013 GO:0080134 GO:0098542 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00902 ko00909 ko01100 ko01110 ko01130 map00902 map00909 map01100 map01110 map01130

Pfam Domains

Protein Families

Terpene_synth Terpene_synth_C

Protein Analysis

535

Amino Acids

60.82

Weight (kDa)

5.48

Isoelectric Point (pI)

39.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Terpene_synth	PF01397	19 - 166	1.1e-37	Terpene synthase, N-terminal domain
Terpene_synth_C	PF03936	226 - 467	2.5e-76	Terpene synthase family, metal binding domain
Terpene_syn_C_2	PF19086	266 - 467	2.5e-50	Terpene synthase family 2, C-terminal metal binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000720)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61680 AT1G61680
fragaria_vesca	FvH4_3g03042 FvH4_3g03042 FvH4_3g03045 FvH4_3g03061 FvH4_3g03072 FvH4_3g03072 FvH4_3g03081 FvH4_3g03150 FvH4_3g03150
malus_domestica	MD05G1334800.v1.1 MD05G1335000.v1.1 MD05G1335100.v1.1 MD10G1309700.v1.1 MD10G1309800.v1.1 MD10G1309900.v1.1 MD10G1310200.v1.1 MD10G1310300.v1.1 MD10G1310500.v1.1 MD10G1310600.v1.1
prunus_persica	Prupe.4G030300_v2.0.a1 Prupe.4G030400_v2.0.a1
pyrus_communis	pycom05g30640 pycom10g26110 pycom10g26140 pycom10g26150 pycom10g26160
rosa_chinensis	RchiOBHm_Chr5g0004711 RchiOBHm_Chr5g0004731 RchiOBHm_Chr5g0004761 RchiOBHm_Chr5g0004801
rosa_laevigata	RLG00000030230 RLG00000031254 RLG00000031255 RLG00000031257 RLG00000031260
rosa_multiflora	Rmu_co8094102.1_g000001 Rmu_sc0002210.1_g000005 Rmu_sc0002210.1_g000008 Rmu_sc0002210.1_g000009 Rmu_sc0008308.1_g000013 Rmu_sc0010871.1_g000028 Rmu_sc0030338.1_g000001
rosa_roxburghii	Rroxscaffold_1G00070820 Rroxscaffold_1G00070840 Rroxscaffold_1G00070850
rosa_rugosa	Rorug04G0413500 Rorug04G0413500 Rorug04G0413700 Rorug04G0413800 Rorug04G0413900 Rorug05G0165300
rosa_samantha	Rh5AG041200 Rh5AG041300 Rh5BG040900 Rh5BG041000 Rh5BG041100 Rh5BG041300 Rh5CG044700 Rh5CG044800 Rh5CG045200 Rh5DG040900 Rh5DG041000 Rh5DG041200 Rh5DG041700 Rh5DG265100
rosa_wichuraiana	Rw5G003960 Rw5G003980 Rw5G003990 Rw5G004000 Rw5G004010 Rw5G004040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1503
AbsI	CCTCGAGG	1 cut(s)	1529
Acc16I	TGCGCA	1 cut(s)	1572
AccB1I	GGYRCC	2 cut(s)	845, 1132
AccB7I	CCANNNNNTGG	1 cut(s)	672
AccI	GTMKAC	3 cut(s)	348, 734, 813
AccII	CGCG	2 cut(s)	497, 1063
AciI	CCGC	5 cut(s)	137, 357, 598, 1077, 1239
AclWI	GGATC	1 cut(s)	755
AcoI	YGGCCR	1 cut(s)	740
AcsI	RAATTY	1 cut(s)	338
AcuI	CTGAAG	2 cut(s)	44, 471
AcyI	GRCGYC	1 cut(s)	144
AfaI	GTAC	4 cut(s)	214, 500, 1103, 1194
AfiI	CCNNNNNNNGG	1 cut(s)	672
AflII	CTTAAG	1 cut(s)	1579
AhlI	ACTAGT	1 cut(s)	361
AjnI	CCWGG	1 cut(s)	397
AloI	GAACNNNNNNTCC	2 cut(s)	689, 721
Alw21I	GWGCWC	1 cut(s)	984
Alw26I	GTCTC	4 cut(s)	274, 387, 401, 708
Alw44I	GTGCAC	1 cut(s)	980
AlwI	GGATC	1 cut(s)	755
Ama87I	CYCGRG	1 cut(s)	1529
AoxI	GGCC	1 cut(s)	740
ApaLI	GTGCAC	1 cut(s)	980
ApeKI	GCWGC	3 cut(s)	875, 1549, 1593
ApoI	RAATTY	1 cut(s)	338
ArsI	GACNNNNNNTTYG	2 cut(s)	815, 847
Asp700I	GAANNNNTTC	1 cut(s)	861
AspA2I	CCTAGG	1 cut(s)	157
AspLEI	GCGC	3 cut(s)	1063, 1573, 1593
AspS9I	GGNCC	2 cut(s)	1288, 1483
AsuHPI	GGTGA	1 cut(s)	920
AsuII	TTCGAA	1 cut(s)	567
AsuNHI	GCTAGC	1 cut(s)	604
AvaI	CYCGRG	1 cut(s)	1529
AvaII	GGWCC	2 cut(s)	1288, 1483
AvrII	CCTAGG	1 cut(s)	157
BaeGI	GKGCMC	3 cut(s)	848, 984, 1083
BanI	GGYRCC	2 cut(s)	845, 1132
BarI	GAAGNNNNNNTAC	2 cut(s)	205, 237
BauI	CACGAG	1 cut(s)	1362
Bbv12I	GWGCWC	1 cut(s)	984
BbvI	GCAGC	2 cut(s)	862, 1536
BccI	CCATC	5 cut(s)	882, 990, 1059, 1289, 1298
BciT130I	CCWGG	1 cut(s)	399
BciVI	GTATCC	1 cut(s)	673
BclI	TGATCA	1 cut(s)	475
BcoDI	GTCTC	4 cut(s)	274, 387, 401, 708
BcuI	ACTAGT	1 cut(s)	361
BfaI	CTAG	9 cut(s)	158, 362, 605, 614, 683, 1007, 1055, 1221, 1476
BfmI	CTRYAG	1 cut(s)	876
BfrI	CTTAAG	1 cut(s)	1579
BfuI	GTATCC	1 cut(s)	673
BisI	GCNGC	3 cut(s)	876, 1550, 1594
BlnI	CCTAGG	1 cut(s)	157
BlsI	GCNGC	3 cut(s)	877, 1551, 1595
BmcAI	AGTACT	1 cut(s)	1103
Bme1390I	CCNGG	1 cut(s)	399
Bme18I	GGWCC	2 cut(s)	1288, 1483
BmeT110I	CYCGRG	1 cut(s)	1529
BmgT120I	GGNCC	2 cut(s)	1288, 1483
BmiI	GGNNCC	4 cut(s)	847, 1001, 1134, 1485
BmrFI	CCNGG	1 cut(s)	399
BmrI	ACTGGG	1 cut(s)	1175
BmsI	GCATC	6 cut(s)	298, 520, 889, 1348, 1436, 1454
BmtI	GCTAGC	1 cut(s)	608
BmuI	ACTGGG	1 cut(s)	1175
Bpu14I	TTCGAA	1 cut(s)	567
Bsa29I	ATCGAT	1 cut(s)	800
BsaAI	YACGTR	3 cut(s)	738, 1196, 1327
BsaBI	GATNNNNATC	1 cut(s)	477
BsaHI	GRCGYC	1 cut(s)	144
BsaI	GGTCTC	1 cut(s)	708
BsaJI	CCNNGG	5 cut(s)	36, 157, 397, 398, 1278
BsaXI	ACNNNNNCTCC	2 cut(s)	689, 719
Bsc4I	CCNNNNNNNGG	1 cut(s)	672
Bse1I	ACTGG	2 cut(s)	910, 1170
Bse3DI	GCAATG	1 cut(s)	215
Bse8I	GATNNNNATC	1 cut(s)	477
BseBI	CCWGG	1 cut(s)	399
BseCI	ATCGAT	1 cut(s)	800
BseDI	CCNNGG	5 cut(s)	36, 157, 397, 398, 1278
BseGI	GGATG	5 cut(s)	462, 511, 1001, 1267, 1288
BseJI	GATNNNNATC	1 cut(s)	477
BseLI	CCNNNNNNNGG	1 cut(s)	672
BseMI	GCAATG	1 cut(s)	215
BseMII	CTCAG	1 cut(s)	269
BseNI	ACTGG	2 cut(s)	910, 1170
BseRI	GAGGAG	2 cut(s)	197, 1547
BseSI	GKGCMC	3 cut(s)	848, 984, 1083
BseXI	GCAGC	2 cut(s)	862, 1536
Bsh1236I	CGCG	2 cut(s)	497, 1063
BshFI	GGCC	1 cut(s)	742
BshNI	GGYRCC	2 cut(s)	845, 1132
BshVI	ATCGAT	1 cut(s)	800
BsiHKAI	GWGCWC	1 cut(s)	984
BsiHKCI	CYCGRG	1 cut(s)	1529
BsiWI	CGTACG	1 cut(s)	1192
BslFI	GGGAC	3 cut(s)	54, 997, 1469
BslI	CCNNNNNNNGG	1 cut(s)	672
BsmAI	GTCTC	4 cut(s)	274, 387, 401, 708
BsmBI	CGTCTC	1 cut(s)	387
BsmFI	GGGAC	3 cut(s)	54, 997, 1469
BsmI	GAATGC	2 cut(s)	1154, 1367
BsnI	GGCC	1 cut(s)	742
Bso31I	GGTCTC	1 cut(s)	708
BsoBI	CYCGRG	1 cut(s)	1529
Bsp119I	TTCGAA	1 cut(s)	567
Bsp1286I	GDGCHC	3 cut(s)	848, 984, 1083
Bsp143I	GATC	3 cut(s)	475, 760, 801
BspACI	CCGC	5 cut(s)	137, 357, 598, 1077, 1239
BspANI	GGCC	1 cut(s)	742
BspCNI	CTCAG	1 cut(s)	270
BspDI	ATCGAT	1 cut(s)	800
BspFNI	CGCG	2 cut(s)	497, 1063
BspLI	GGNNCC	4 cut(s)	847, 1001, 1134, 1485
BspMAI	CTGCAG	1 cut(s)	880
BspOI	GCTAGC	1 cut(s)	608
BspPI	GGATC	1 cut(s)	755
BspT104I	TTCGAA	1 cut(s)	567
BspT107I	GGYRCC	2 cut(s)	845, 1132
BspTI	CTTAAG	1 cut(s)	1579
BspTNI	GGTCTC	1 cut(s)	708
BsrDI	GCAATG	1 cut(s)	215
BsrI	ACTGG	2 cut(s)	910, 1170
BssECI	CCNNGG	5 cut(s)	36, 157, 397, 398, 1278
BssMI	GATC	3 cut(s)	475, 760, 801
BssNAI	GTATAC	1 cut(s)	735
BssNI	GRCGYC	1 cut(s)	144
BssSI	CACGAG	1 cut(s)	1362
BssT1I	CCWWGG	3 cut(s)	36, 157, 1278
Bst1107I	GTATAC	1 cut(s)	735
Bst2BI	CACGAG	1 cut(s)	1362
Bst2UI	CCWGG	1 cut(s)	399
Bst4CI	ACNGT	2 cut(s)	1094, 1192
Bst6I	CTCTTC	3 cut(s)	399, 872, 1092
BstACI	GRCGYC	1 cut(s)	144
BstAFI	CTTAAG	1 cut(s)	1579
BstBAI	YACGTR	3 cut(s)	738, 1196, 1327
BstBI	TTCGAA	1 cut(s)	567
BstC8I	GCNNGC	2 cut(s)	606, 1079
BstDEI	CTNAG	3 cut(s)	278, 546, 693
BstF5I	GGATG	5 cut(s)	462, 511, 1001, 1267, 1288
BstFNI	CGCG	2 cut(s)	497, 1063
BstHHI	GCGC	3 cut(s)	1063, 1573, 1593
BstKTI	GATC	3 cut(s)	478, 763, 804
BstMAI	GTCTC	4 cut(s)	274, 387, 401, 708
BstMBI	GATC	3 cut(s)	475, 760, 801
BstMWI	GCNNNNNNNGC	6 cut(s)	26, 143, 234, 988, 1524, 1590
BstNI	CCWGG	1 cut(s)	399
BstNSI	RCATGY	2 cut(s)	753, 1154
BstSCI	CCNGG	1 cut(s)	397
BstSFI	CTRYAG	1 cut(s)	876
BstSLI	GKGCMC	3 cut(s)	848, 984, 1083
BstSNI	TACGTA	1 cut(s)	1196
BstUI	CGCG	2 cut(s)	497, 1063
BstV1I	GCAGC	2 cut(s)	862, 1536
BstX2I	RGATCY	1 cut(s)	760
BstXI	CCANNNNNNTGG	1 cut(s)	729
BstYI	RGATCY	1 cut(s)	760
BstZ17I	GTATAC	1 cut(s)	735
Bsu15I	ATCGAT	1 cut(s)	800
BsuI	GTATCC	1 cut(s)	673
BsuRI	GGCC	1 cut(s)	742
BsuTUI	ATCGAT	1 cut(s)	800
BtgZI	GCGATG	2 cut(s)	243, 1078
BtsCI	GGATG	5 cut(s)	462, 511, 1001, 1267, 1288
BtsI	GCAGTG	1 cut(s)	244
BtsIMutI	CAGTG	2 cut(s)	244, 1099
Cac8I	GCNNGC	2 cut(s)	606, 1079
CfoI	GCGC	3 cut(s)	1063, 1573, 1593
Cfr13I	GGNCC	2 cut(s)	1288, 1483
ClaI	ATCGAT	1 cut(s)	800
CseI	GACGC	2 cut(s)	152, 369
Csp6I	GTAC	4 cut(s)	213, 499, 1102, 1193
CviAII	CATG	8 cut(s)	71, 148, 296, 625, 750, 925, 992, 1151
CviQI	GTAC	4 cut(s)	213, 499, 1102, 1193
DdeI	CTNAG	3 cut(s)	278, 546, 693
DpnI	GATC	3 cut(s)	477, 762, 803
DpnII	GATC	3 cut(s)	475, 760, 801
DraI	TTTAAA	1 cut(s)	201
EaeI	YGGCCR	1 cut(s)	740
Eam1104I	CTCTTC	3 cut(s)	399, 872, 1092
EarI	CTCTTC	3 cut(s)	399, 872, 1092
Eco105I	TACGTA	1 cut(s)	1196
Eco130I	CCWWGG	3 cut(s)	36, 157, 1278
Eco31I	GGTCTC	1 cut(s)	708
Eco47I	GGWCC	2 cut(s)	1288, 1483
Eco57I	CTGAAG	2 cut(s)	44, 471
Eco88I	CYCGRG	1 cut(s)	1529
EcoRI	GAATTC	1 cut(s)	338
EcoRII	CCWGG	1 cut(s)	397
EcoT14I	CCWWGG	3 cut(s)	36, 157, 1278
EcoT22I	ATGCAT	2 cut(s)	313, 1156
ErhI	CCWWGG	3 cut(s)	36, 157, 1278
Esp3I	CGTCTC	1 cut(s)	387
FaeI	CATG	8 cut(s)	74, 151, 299, 628, 753, 928, 995, 1154
FalI	AAGNNNNNCTT	4 cut(s)	396, 428, 539, 571
FaqI	GGGAC	3 cut(s)	54, 997, 1469
FatI	CATG	8 cut(s)	70, 147, 295, 624, 749, 924, 991, 1150
FauI	CCCGC	2 cut(s)	591, 1070
FbaI	TGATCA	1 cut(s)	475
FblI	GTMKAC	3 cut(s)	348, 734, 813
Fnu4HI	GCNGC	3 cut(s)	876, 1550, 1594
FokI	GGATG	5 cut(s)	469, 498, 1008, 1274, 1295
Fsp4HI	GCNGC	3 cut(s)	876, 1550, 1594
FspBI	CTAG	9 cut(s)	158, 362, 605, 614, 683, 1007, 1055, 1221, 1476
FspI	TGCGCA	1 cut(s)	1572
GlaI	GCGC	3 cut(s)	1062, 1572, 1592
GluI	GCNGC	3 cut(s)	876, 1550, 1594
HaeIII	GGCC	1 cut(s)	742
HgaI	GACGC	2 cut(s)	152, 369
HhaI	GCGC	3 cut(s)	1063, 1573, 1593
Hin1I	GRCGYC	1 cut(s)	144
Hin1II	CATG	8 cut(s)	74, 151, 299, 628, 753, 928, 995, 1154
Hin6I	GCGC	3 cut(s)	1061, 1571, 1591
HinP1I	GCGC	3 cut(s)	1061, 1571, 1591
HincII	GTYRAC	3 cut(s)	142, 166, 349
HindII	GTYRAC	3 cut(s)	142, 166, 349
HindIII	AAGCTT	2 cut(s)	483, 766
HinfI	GANTC	3 cut(s)	537, 632, 1010
HphI	GGTGA	1 cut(s)	920
Hpy166II	GTNNAC	8 cut(s)	142, 166, 349, 735, 814, 911, 982, 1204
Hpy188I	TCNGA	6 cut(s)	116, 644, 775, 1351, 1390, 1558
Hpy188III	TCNNGA	4 cut(s)	325, 470, 709, 1007
Hpy8I	GTNNAC	8 cut(s)	142, 166, 349, 735, 814, 911, 982, 1204
HpyAV	CCTTC	8 cut(s)	23, 68, 79, 88, 227, 420, 563, 1529
HpyCH4III	ACNGT	2 cut(s)	1094, 1192
HpyCH4IV	ACGT	3 cut(s)	737, 1195, 1326
HpyF10VI	GCNNNNNNNGC	6 cut(s)	26, 143, 234, 988, 1524, 1590
HpyF3I	CTNAG	3 cut(s)	278, 546, 693
HpySE526I	ACGT	3 cut(s)	737, 1195, 1326
Hsp92I	GRCGYC	1 cut(s)	144
Hsp92II	CATG	8 cut(s)	74, 151, 299, 628, 753, 928, 995, 1154
HspAI	GCGC	3 cut(s)	1061, 1571, 1591
Ksp22I	TGATCA	1 cut(s)	475
Kzo9I	GATC	3 cut(s)	475, 760, 801
LmnI	GCTCC	1 cut(s)	601
Lsp1109I	GCAGC	2 cut(s)	862, 1536
LweI	GCATC	6 cut(s)	298, 520, 889, 1348, 1436, 1454
MaeI	CTAG	9 cut(s)	158, 362, 605, 614, 683, 1007, 1055, 1221, 1476
MaeII	ACGT	3 cut(s)	737, 1195, 1326
MaeIII	GTNAC	2 cut(s)	298, 1322
MalI	GATC	3 cut(s)	477, 762, 803
MboI	GATC	3 cut(s)	475, 760, 801
MboII	GAAGA	9 cut(s)	416, 859, 940, 1109, 1150, 1224, 1241, 1367, 1403
MfeI	CAATTG	1 cut(s)	128
MflI	RGATCY	1 cut(s)	760
MhlI	GDGCHC	3 cut(s)	848, 984, 1083
MlyI	GAGTC	1 cut(s)	1019
Mph1103I	ATGCAT	2 cut(s)	313, 1156
MroXI	GAANNNNTTC	1 cut(s)	861
MseI	TTAA	8 cut(s)	200, 315, 557, 726, 939, 1017, 1467, 1580
MspA1I	CMGCKG	1 cut(s)	875
MspCI	CTTAAG	1 cut(s)	1579
MspR9I	CCNGG	1 cut(s)	399
MunI	CAATTG	1 cut(s)	128
Mva1269I	GAATGC	2 cut(s)	1154, 1367
MvaI	CCWGG	1 cut(s)	399
MvnI	CGCG	2 cut(s)	497, 1063
MwoI	GCNNNNNNNGC	6 cut(s)	26, 143, 234, 988, 1524, 1590
NdeII	GATC	3 cut(s)	475, 760, 801
NheI	GCTAGC	1 cut(s)	604
NlaIII	CATG	8 cut(s)	74, 151, 299, 628, 753, 928, 995, 1154
NlaIV	GGNNCC	4 cut(s)	847, 1001, 1134, 1485
NsbI	TGCGCA	1 cut(s)	1572
NsiI	ATGCAT	2 cut(s)	313, 1156
NspI	RCATGY	2 cut(s)	753, 1154
NspV	TTCGAA	1 cut(s)	567
PaeR7I	CTCGAG	1 cut(s)	1529
PasI	CCCWGGG	1 cut(s)	398
PctI	GAATGC	2 cut(s)	1154, 1367
PdmI	GAANNNNTTC	1 cut(s)	861
PfeI	GAWTC	2 cut(s)	537, 632
Pfl23II	CGTACG	1 cut(s)	1192
PflMI	CCANNNNNTGG	1 cut(s)	672
PkrI	GCNGC	3 cut(s)	877, 1551, 1595
PleI	GAGTC	1 cut(s)	1018
PpsI	GAGTC	1 cut(s)	1018
Ppu21I	YACGTR	3 cut(s)	738, 1196, 1327
PsiI	TTATAA	1 cut(s)	1503
Psp6I	CCWGG	1 cut(s)	397
PspGI	CCWGG	1 cut(s)	397
PspLI	CGTACG	1 cut(s)	1192
PspN4I	GGNNCC	4 cut(s)	847, 1001, 1134, 1485
PspPI	GGNCC	2 cut(s)	1288, 1483
PspXI	VCTCGAGB	1 cut(s)	1529
PstI	CTGCAG	1 cut(s)	880
PsuI	RGATCY	1 cut(s)	760
PvuII	CAGCTG	1 cut(s)	875
RsaI	GTAC	4 cut(s)	214, 500, 1103, 1194
RsaNI	GTAC	4 cut(s)	213, 499, 1102, 1193
SalI	GTCGAC	1 cut(s)	347
SaqAI	TTAA	8 cut(s)	200, 315, 557, 726, 939, 1017, 1467, 1580
SatI	GCNGC	3 cut(s)	876, 1550, 1594
Sau3AI	GATC	3 cut(s)	475, 760, 801
Sau96I	GGNCC	2 cut(s)	1288, 1483
ScaI	AGTACT	1 cut(s)	1103
SchI	GAGTC	1 cut(s)	1019
ScrFI	CCNGG	1 cut(s)	399
SduI	GDGCHC	3 cut(s)	848, 984, 1083
SfaNI	GCATC	6 cut(s)	298, 520, 889, 1348, 1436, 1454
SfcI	CTRYAG	1 cut(s)	876
Sfr274I	CTCGAG	1 cut(s)	1529
SfuI	TTCGAA	1 cut(s)	567
SinI	GGWCC	2 cut(s)	1288, 1483
SlaI	CTCGAG	1 cut(s)	1529
SmlI	CTYRAG	2 cut(s)	1529, 1579
SmoI	CTYRAG	2 cut(s)	1529, 1579
SnaBI	TACGTA	1 cut(s)	1196
SpeI	ACTAGT	1 cut(s)	361
SsiI	CCGC	5 cut(s)	137, 357, 598, 1077, 1239
SspMI	CTAG	9 cut(s)	158, 362, 605, 614, 683, 1007, 1055, 1221, 1476
StyD4I	CCNGG	1 cut(s)	397
StyI	CCWWGG	3 cut(s)	36, 157, 1278
TaaI	ACNGT	2 cut(s)	1094, 1192
TaiI	ACGT	3 cut(s)	740, 1198, 1329
TaqI	TCGA	7 cut(s)	271, 348, 471, 567, 800, 852, 1530
TatI	WGTACW	1 cut(s)	1101
TfiI	GAWTC	2 cut(s)	537, 632
Tru1I	TTAA	8 cut(s)	200, 315, 557, 726, 939, 1017, 1467, 1580
Tru9I	TTAA	8 cut(s)	200, 315, 557, 726, 939, 1017, 1467, 1580
TscAI	CASTG	2 cut(s)	244, 1099
TseI	GCWGC	3 cut(s)	875, 1549, 1593
TspRI	CASTG	2 cut(s)	244, 1099
Van91I	CCANNNNNTGG	1 cut(s)	672
Vha464I	CTTAAG	1 cut(s)	1579
VneI	GTGCAC	1 cut(s)	980
VpaK11BI	GGWCC	2 cut(s)	1288, 1483
XapI	RAATTY	1 cut(s)	338
XbaI	TCTAGA	1 cut(s)	1006
XceI	RCATGY	2 cut(s)	753, 1154
XhoI	CTCGAG	1 cut(s)	1529
XmaJI	CCTAGG	1 cut(s)	157
XmiI	GTMKAC	3 cut(s)	348, 734, 813
XmnI	GAANNNNTTC	1 cut(s)	861
XspI	CTAG	9 cut(s)	158, 362, 605, 614, 683, 1007, 1055, 1221, 1476
ZrmI	AGTACT	1 cut(s)	1103
Zsp2I	ATGCAT	2 cut(s)	313, 1156

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.