Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0001310

Physical Location & Seq

Genomic Coordinates Forward (+)

11384309 .. 11387535

Gene Structure 3227 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1145800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2037 bp

ATGTCTGCCGCCTCTTCGCTGCAATCGAGAGCTGCCGGCGGCACCAAACCGGATATTGACGCCGCAAACGAAGCCTTGAACCCGATGGACGAGGACCTTAAGGTCTCTAGCAGCGTTGGCGCGCTTAAAGTCACTAGGCAGGTGCCATCTCGTCAAGCCATGGCGAAAATGCTGCAAGAGCGGTTGTTTTACATCCCGTCCTTCAAAATCTACGACGGCGCTGCGGAAACCGCTGGGCTTTTCGACTACGGGCCTCCCGGCGTCAAGTTGAAGAACAACGTTCTTGCATTTTGGCGTCAGCATTTTGTTCTTGGGGAGAACATGTTAGAAGTCGAGTGCCCGTCTGTCACGCCCGAGATCGTTCTGAAAGCGTCGGGGCACGTAGACAGATTCACTGACCTTATGGTTAAGGACGAGAAGAACGGGAACTGCTACCGCGTAGACCACTTGCTGAAGGATTTCTGCACCGAGAAGCTTCAGAAGGATCTCAACATCGCCGAAGAGAAGGCGAAGGAACTCAAACATGTACTCGCTCTGTTAGATGACCTCTCAGCTGAAGAACTAGGTGCAAAGATCAAAGAGTATGGGATTACAGCCCCTGACACGAGCAATCCTCTGTCTGATCCCTATCCATTTAACTTAATGTTCCAAACGTCAATTGGTCCGTCTGGCTCGAGCACTGCGTACATGAGACCGGAAACTGCACAAGGCATATTTGTGAACTTCAACTATTTGTACTACTACAATGGCAACAAGCTACCTTTTGCTGCGGCTCAAATCGGCCAAGCCTTTCGAAATGAGATTGCTCCTCGCCAAGGGCTTTTAAGAGTCCGTGAGTTCACACTCGCGGAGATTGAACACTTTGTAGATCCTGAAGACAAAACTCACCCGAAGTTTAATGAGGTCGCGGAGTTGGAGTTCCAAATGTTTCCGAGGGAAGAACAGGTATCTGGCCAGTCTGCAAGGATAATCCGCTTGGGTGAAGCAGTTGCAAAGGGCATAGTCGACAATCAGACTTTAGCCTATTTCATCGGCCGAGTCTACCTCTTCCTCACACGGTTGGGTATAGACGAGGAGCGTCTAAGGTTCCGCCAGCACCTGCCGAATGAAATGGCGCACTACGCTGCTGACTGCTGGGATGCTGAGATCGAGACTTCATACGGGTGGATTGAGTGCGTCGGGATAGCAGATAGATCTGCATATGATCTTCAAGCTCATTCAGTAAAAAGTAATAAAGATCTTATGGCTCATGAAAAATTTTCAGAACCAAGAGAAGTGGAGGAACTGGTTATATCTCCGGTGGTTAAAGAGACCGGCCTTGCTTTCAAGGCGGACGGAAAGAATGTAGTTGAAGCACTGAAGGCCATGAATGAAAAAGAAGCTTTGGAGATGAAAGCGGATTTAGAGGCGAAGGGCGAGGTGGAGTTTTATGTCTGCACACTAGGGAAAAATGTATCAATAAAGCAGAATATGGTGACGATTTCATACAAGAAAAGGAAAGAACACCAGAGAAGATTCACACCCTCAGTGATCGAACCTTCGTTTGGTATCGGGCGGATCATCTACTGCCTCTTCGAGCATTCATACTACACCAGACAAAGTCAAGCTGGGGATGAACAGTCGAACAACTTGAACGTGCTCCGTTTCCCTCCTCTCATGGCGCCCTTTAAGTGCACGGTTTTCCCGCTGGTTCAGAACAAGGAGTATGAGGAGGTTGCTAAAGGCATTTCCAAGTCACTGACCGCAGCTGGCATATCACATAAAATCGACATTACAGGCACCTCTATCGGGAAAAAGTATGCACGGACTGACGAGCTTGGTGCGCCCTTCGCGATCACGGTGGATTCTACAACGTCAGTGACAATTCGGGAGAGAGACAGCAAGGATCAGGTTCGTGTAAATTTGAAAGAGGTAGTGGTCGTTGTGAAGGAGCTAACTGCGGGGCTTAAGACTTGGGCAGACGTGTCGCGTGCTCACAGAGTCAGAAGGCAATCTAGGCTTAGAAATTTACTTAACAAATTGTGTTTTCCCTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0003674 GO:0003824 GO:0004812 GO:0004820 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006426 GO:0006518 GO:0006520 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009117 GO:0009165 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0012505 GO:0015959 GO:0015960 GO:0015965 GO:0015966 GO:0016070 GO:0016874 GO:0016875 GO:0018130 GO:0019438 GO:0019538 GO:0019637 GO:0019752 GO:0031410 GO:0031982 GO:0031984 GO:0032543 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046686 GO:0046983 GO:0050896 GO:0055086 GO:0070127 GO:0070150 GO:0071704 GO:0090304 GO:0090407 GO:0097708 GO:0098791 GO:0140053 GO:0140098 GO:0140101 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon WHEP-TRS tRNA-synt_2b

Protein Analysis

679

Amino Acids

76.22

Weight (kDa)

6.33

Isoelectric Point (pI)

40.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2b	PF00587	228 - 527	7.4e-10	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	557 - 644	1.2e-19	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29880
fragaria_vesca	FvH4_4g22441 FvH4_4g22450 FvH4_4g22450 FvH4_4g22610 FvH4_4g22610
malus_domestica	MD13G1131000.v1.1 MD13G1145800.v1.1 MD16G1141200.v1.1
prunus_persica	Prupe.1G214600_v2.0.a1
pyrus_communis	pycom13g11430 pycom13g12530 pycom16g11160 pycom16g11170
rosa_chinensis	RchiOBHm_Chr1g0361881 RchiOBHm_Chr4g0428711 RchiOBHm_Chr4g0428851
rosa_laevigata	RLG00000007120 RLG00000007133 RLG00000027689 RLG00000027690 RLG00000027694
rosa_multiflora	Rmu_co8208336.1_g000001 Rmu_co8210234.1_g000001 Rmu_sc0003316.1_g000006 Rmu_sc0008612.1_g000003 Rmu_sc0018216.1_g000001 Rmu_ssc0000124.1_g000013
rosa_roxburghii	Rroxscaffold_4G00294630 Rroxscaffold_5G00370530
rosa_rugosa	Rorug01G0292100 Rorug04G0227400 Rorug04G0227500 Rorug04G0229700
rosa_samantha	Rh1AG302800 Rh1BG265900 Rh1BG266000 Rh1CG284000 Rh1DG295900 Rh1DG296000 Rh4AG284700 Rh4AG284900 Rh4AG286400 Rh4BG290700 Rh4BG290900 Rh4BG292200 Rh4CG308200 Rh4DG288200 Rh4DG289400
rosa_wichuraiana	Rw0G020620 Rw1G026830 Rw4G024720 Rw4G024830 Rw7G040280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	130, 1107
AasI	GACNNNNNNGTC	1 cut(s)	101
Acc36I	ACCTGC	2 cut(s)	130, 1107
AccB1I	GGYRCC	4 cut(s)	41, 142, 1660, 1778
AccBSI	CCGCTC	1 cut(s)	181
AccI	GTMKAC	4 cut(s)	384, 441, 1005, 1041
AccII	CGCG	6 cut(s)	122, 438, 848, 908, 1832, 1969
AclI	AACGTT	1 cut(s)	279
AclWI	GGATC	5 cut(s)	492, 617, 863, 1565, 1893
AcoI	YGGCCR	3 cut(s)	781, 952, 1033
AcsI	RAATTY	3 cut(s)	1256, 1900, 2005
AcuI	CTGAAG	5 cut(s)	461, 473, 576, 894, 1379
AcyI	GRCGYC	4 cut(s)	60, 261, 295, 1661
AfaI	GTAC	3 cut(s)	528, 686, 737
AfiI	CCNNNNNNNGG	3 cut(s)	815, 1544, 1788
AflII	CTTAAG	2 cut(s)	98, 1946
AflIII	ACRYGT	3 cut(s)	321, 523, 1962
AjiI	CACGTC	1 cut(s)	1963
AjuI	GAANNNNNNNTTGG	2 cut(s)	1527, 1559
AloI	GAACNNNNNNTCC	2 cut(s)	902, 934
Alw21I	GWGCWC	4 cut(s)	680, 1641, 1676, 1975
Alw26I	GTCTC	5 cut(s)	109, 685, 1145, 1304, 1869
Alw44I	GTGCAC	1 cut(s)	1672
AlwI	GGATC	5 cut(s)	492, 617, 863, 1565, 1893
AlwNI	CAGNNNCTG	2 cut(s)	599, 1099
Ama87I	CYCGRG	2 cut(s)	353, 673
AoxI	GGCC	6 cut(s)	251, 781, 952, 1033, 1315, 1362
ApaLI	GTGCAC	1 cut(s)	1672
ApeKI	GCWGC	8 cut(s)	19, 32, 111, 172, 221, 767, 1124, 1745
ApoI	RAATTY	3 cut(s)	1256, 1900, 2005
Asp700I	GAANNNNTTC	1 cut(s)	458
AspLEI	GCGC	6 cut(s)	122, 124, 221, 1117, 1663, 1825
AspS9I	GGNCC	3 cut(s)	94, 251, 662
AsuC2I	CCSGG	1 cut(s)	258
AsuHPI	GGTGA	3 cut(s)	878, 992, 1486
AsuII	TTCGAA	1 cut(s)	793
AvaI	CYCGRG	2 cut(s)	353, 673
AvaII	GGWCC	2 cut(s)	94, 662
BaeGI	GKGCMC	3 cut(s)	341, 381, 1676
BalI	TGGCCA	1 cut(s)	954
BanI	GGYRCC	4 cut(s)	41, 142, 1660, 1778
BauI	CACGAG	1 cut(s)	604
BbsI	GAAGAC	1 cut(s)	882
Bbv12I	GWGCWC	4 cut(s)	680, 1641, 1676, 1975
BbvI	GCAGC	8 cut(s)	6, 19, 123, 159, 208, 754, 1111, 1757
BccI	CCATC	2 cut(s)	79, 154
BceAI	ACGGC	1 cut(s)	232
BcnI	CCSGG	1 cut(s)	258
BcoDI	GTCTC	5 cut(s)	109, 685, 1145, 1304, 1869
BfaI	CTAG	5 cut(s)	108, 135, 563, 1442, 1995
BfoI	RGCGCY	2 cut(s)	222, 1664
BfrI	CTTAAG	2 cut(s)	98, 1946
BfuAI	ACCTGC	2 cut(s)	130, 1107
BglII	AGATCT	2 cut(s)	1193, 1237
Bme1390I	CCNGG	1 cut(s)	258
Bme18I	GGWCC	2 cut(s)	94, 662
BmeT110I	CYCGRG	2 cut(s)	353, 673
BmgBI	CACGTC	1 cut(s)	1963
BmgT120I	GGNCC	3 cut(s)	94, 251, 662
BmiI	GGNNCC	5 cut(s)	43, 144, 1088, 1662, 1780
BmrFI	CCNGG	1 cut(s)	258
BmsI	GCATC	1 cut(s)	1129
BpiI	GAAGAC	1 cut(s)	882
BplI	GAGNNNNNCTC	4 cut(s)	598, 630, 1029, 1061
Bpu14I	TTCGAA	1 cut(s)	793
BpuMI	CCSGG	1 cut(s)	258
BsaAI	YACGTR	1 cut(s)	382
BsaBI	GATNNNNATC	1 cut(s)	627
BsaHI	GRCGYC	4 cut(s)	60, 261, 295, 1661
BsaI	GGTCTC	3 cut(s)	109, 685, 1304
BsaJI	CCNNGG	3 cut(s)	159, 814, 932
BsaWI	WCCGGW	3 cut(s)	49, 694, 1297
BsaXI	ACNNNNNCTCC	4 cut(s)	308, 338, 902, 932
Bsc4I	CCNNNNNNNGG	3 cut(s)	815, 1544, 1788
Bse118I	RCCGGY	2 cut(s)	35, 1313
Bse1I	ACTGG	2 cut(s)	955, 1290
Bse8I	GATNNNNATC	1 cut(s)	627
BseDI	CCNNGG	3 cut(s)	159, 814, 932
BseGI	GGATG	3 cut(s)	192, 1144, 1618
BseJI	GATNNNNATC	1 cut(s)	627
BseLI	CCNNNNNNNGG	3 cut(s)	815, 1544, 1788
BseMII	CTCAG	3 cut(s)	564, 1134, 1539
BseNI	ACTGG	2 cut(s)	955, 1290
BsePI	GCGCGC	1 cut(s)	120
BseRI	GAGGAG	4 cut(s)	798, 1088, 1641, 1724
BseSI	GKGCMC	3 cut(s)	341, 381, 1676
BseX3I	CGGCCG	1 cut(s)	1033
BseXI	GCAGC	8 cut(s)	6, 19, 123, 159, 208, 754, 1111, 1757
BseYI	CCCAGC	3 cut(s)	233, 1134, 1607
BsgI	GTGCAG	3 cut(s)	448, 687, 1420
Bsh1236I	CGCG	6 cut(s)	122, 438, 848, 908, 1832, 1969
Bsh1285I	CGRYCG	1 cut(s)	1036
BshFI	GGCC	6 cut(s)	253, 783, 954, 1035, 1317, 1364
BshNI	GGYRCC	4 cut(s)	41, 142, 1660, 1778
BsiEI	CGRYCG	1 cut(s)	1036
BsiHKAI	GWGCWC	4 cut(s)	680, 1641, 1676, 1975
BsiHKCI	CYCGRG	2 cut(s)	353, 673
BsiSI	CCGG	6 cut(s)	36, 50, 258, 695, 1298, 1314
BslI	CCNNNNNNNGG	3 cut(s)	815, 1544, 1788
BsmAI	GTCTC	5 cut(s)	109, 685, 1145, 1304, 1869
BsmI	GAATGC	1 cut(s)	1579
BsnI	GGCC	6 cut(s)	253, 783, 954, 1035, 1317, 1364
Bso31I	GGTCTC	3 cut(s)	109, 685, 1304
BsoBI	CYCGRG	2 cut(s)	353, 673
Bsp119I	TTCGAA	1 cut(s)	793
Bsp1286I	GDGCHC	6 cut(s)	341, 381, 680, 1641, 1676, 1975
Bsp19I	CCATGG	1 cut(s)	159
Bsp68I	TCGCGA	1 cut(s)	1832
BspANI	GGCC	6 cut(s)	253, 783, 954, 1035, 1317, 1364
BspCNI	CTCAG	3 cut(s)	563, 1135, 1538
BspFNI	CGCG	6 cut(s)	122, 438, 848, 908, 1832, 1969
BspHI	TCATGA	1 cut(s)	1249
BspLI	GGNNCC	5 cut(s)	43, 144, 1088, 1662, 1780
BspMI	ACCTGC	2 cut(s)	130, 1107
BspPI	GGATC	5 cut(s)	492, 617, 863, 1565, 1893
BspT104I	TTCGAA	1 cut(s)	793
BspT107I	GGYRCC	4 cut(s)	41, 142, 1660, 1778
BspTI	CTTAAG	2 cut(s)	98, 1946
BspTNI	GGTCTC	3 cut(s)	109, 685, 1304
BsrBI	CCGCTC	1 cut(s)	181
BsrFI	RCCGGY	2 cut(s)	35, 1313
BsrI	ACTGG	2 cut(s)	955, 1290
BssAI	RCCGGY	2 cut(s)	35, 1313
BssECI	CCNNGG	3 cut(s)	159, 814, 932
BssHII	GCGCGC	1 cut(s)	120
BssNI	GRCGYC	4 cut(s)	60, 261, 295, 1661
BssSI	CACGAG	1 cut(s)	604
BssT1I	CCWWGG	2 cut(s)	159, 814
Bst2BI	CACGAG	1 cut(s)	604
Bst4CI	ACNGT	4 cut(s)	1059, 1620, 1678, 1840
Bst6I	CTCTTC	4 cut(s)	19, 495, 1052, 1577
BstACI	GRCGYC	4 cut(s)	60, 261, 295, 1661
BstAFI	CTTAAG	2 cut(s)	98, 1946
BstBAI	YACGTR	1 cut(s)	382
BstBI	TTCGAA	1 cut(s)	793
BstC8I	GCNNGC	5 cut(s)	37, 122, 1094, 1750, 1971
BstDEI	CTNAG	5 cut(s)	550, 1082, 1143, 1525, 2000
BstDSI	CCRYGG	1 cut(s)	159
BstENI	CCTNNNNNAGG	1 cut(s)	813
BstF5I	GGATG	3 cut(s)	192, 1144, 1618
BstFNI	CGCG	6 cut(s)	122, 438, 848, 908, 1832, 1969
BstH2I	RGCGCY	2 cut(s)	222, 1664
BstHHI	GCGC	6 cut(s)	122, 124, 221, 1117, 1663, 1825
BstMAI	GTCTC	5 cut(s)	109, 685, 1145, 1304, 1869
BstMCI	CGRYCG	1 cut(s)	1036
BstNSI	RCATGY	2 cut(s)	325, 527
BstSCI	CCNGG	1 cut(s)	256
BstSLI	GKGCMC	3 cut(s)	341, 381, 1676
BstUI	CGCG	6 cut(s)	122, 438, 848, 908, 1832, 1969
BstV1I	GCAGC	8 cut(s)	6, 19, 123, 159, 208, 754, 1111, 1757
BstV2I	GAAGAC	1 cut(s)	882
BstX2I	RGATCY	4 cut(s)	484, 868, 1193, 1237
BstYI	RGATCY	4 cut(s)	484, 868, 1193, 1237
BstZI	CGGCCG	1 cut(s)	1033
BsuRI	GGCC	6 cut(s)	253, 783, 954, 1035, 1317, 1364
BtgI	CCRYGG	1 cut(s)	159
BtgZI	GCGATG	1 cut(s)	478
BtrI	CACGTC	1 cut(s)	1963
BtsCI	GGATG	3 cut(s)	192, 1144, 1618
BtsI	GCAGTG	1 cut(s)	678
BtsIMutI	CAGTG	6 cut(s)	393, 678, 1355, 1533, 1736, 1863
BtuMI	TCGCGA	1 cut(s)	1832
BveI	ACCTGC	2 cut(s)	130, 1107
Cac8I	GCNNGC	5 cut(s)	37, 122, 1094, 1750, 1971
CaiI	CAGNNNCTG	2 cut(s)	599, 1099
CciI	TCATGA	1 cut(s)	1249
CfoI	GCGC	6 cut(s)	122, 124, 221, 1117, 1663, 1825
Cfr10I	RCCGGY	2 cut(s)	35, 1313
Cfr13I	GGNCC	3 cut(s)	94, 251, 662
CseI	GACGC	6 cut(s)	68, 250, 284, 360, 1067, 1165
Csp6I	GTAC	3 cut(s)	527, 685, 736
CspCI	CAANNNNNGTGG	2 cut(s)	1257, 1292
CviAII	CATG	7 cut(s)	160, 322, 524, 688, 1250, 1366, 1657
CviQI	GTAC	3 cut(s)	527, 685, 736
DdeI	CTNAG	5 cut(s)	550, 1082, 1143, 1525, 2000
DinI	GGCGCC	1 cut(s)	1662
DrdI	GACNNNNNNGTC	1 cut(s)	101
DseDI	GACNNNNNNGTC	1 cut(s)	101
EaeI	YGGCCR	3 cut(s)	781, 952, 1033
EagI	CGGCCG	1 cut(s)	1033
Eam1104I	CTCTTC	4 cut(s)	19, 495, 1052, 1577
EarI	CTCTTC	4 cut(s)	19, 495, 1052, 1577
EciI	GGCGGA	3 cut(s)	1079, 1346, 1570
EclXI	CGGCCG	1 cut(s)	1033
Eco130I	CCWWGG	2 cut(s)	159, 814
Eco31I	GGTCTC	3 cut(s)	109, 685, 1304
Eco47I	GGWCC	2 cut(s)	94, 662
Eco52I	CGGCCG	1 cut(s)	1033
Eco57I	CTGAAG	5 cut(s)	461, 473, 576, 894, 1379
Eco88I	CYCGRG	2 cut(s)	353, 673
EcoNI	CCTNNNNNAGG	1 cut(s)	813
EcoO109I	RGGNCCY	1 cut(s)	94
EcoT14I	CCWWGG	2 cut(s)	159, 814
EgeI	GGCGCC	1 cut(s)	1662
EheI	GGCGCC	1 cut(s)	1662
ErhI	CCWWGG	2 cut(s)	159, 814
FaeI	CATG	7 cut(s)	163, 325, 527, 691, 1253, 1369, 1660
FatI	CATG	7 cut(s)	159, 321, 523, 687, 1249, 1365, 1656
FauI	CCCGC	2 cut(s)	1692, 1933
FauNDI	CATATG	1 cut(s)	1201
FblI	GTMKAC	4 cut(s)	384, 441, 1005, 1041
FokI	GGATG	3 cut(s)	179, 1151, 1625
FspBI	CTAG	5 cut(s)	108, 135, 563, 1442, 1995
GlaI	GCGC	6 cut(s)	121, 123, 220, 1116, 1662, 1824
GsaI	CCCAGC	3 cut(s)	237, 1138, 1611
HaeII	RGCGCY	2 cut(s)	222, 1664
HaeIII	GGCC	6 cut(s)	253, 783, 954, 1035, 1317, 1364
HapII	CCGG	6 cut(s)	36, 50, 258, 695, 1298, 1314
HgaI	GACGC	6 cut(s)	68, 250, 284, 360, 1067, 1165
HhaI	GCGC	6 cut(s)	122, 124, 221, 1117, 1663, 1825
Hin1I	GRCGYC	4 cut(s)	60, 261, 295, 1661
Hin1II	CATG	7 cut(s)	163, 325, 527, 691, 1253, 1369, 1660
Hin6I	GCGC	6 cut(s)	120, 122, 219, 1115, 1661, 1823
HinP1I	GCGC	6 cut(s)	120, 122, 219, 1115, 1661, 1823
HincII	GTYRAC	1 cut(s)	1006
HindII	GTYRAC	1 cut(s)	1006
HindIII	AAGCTT	2 cut(s)	473, 1380
HinfI	GANTC	6 cut(s)	390, 828, 1038, 1515, 1844, 1980
HpaII	CCGG	6 cut(s)	36, 50, 258, 695, 1298, 1314
HphI	GGTGA	3 cut(s)	878, 992, 1486
Hpy166II	GTNNAC	7 cut(s)	385, 442, 721, 840, 1006, 1042, 1674
Hpy188I	TCNGA	8 cut(s)	366, 480, 622, 933, 1014, 1264, 1695, 1985
Hpy188III	TCNNGA	8 cut(s)	27, 872, 1150, 1180, 1250, 1789, 1831, 1868
Hpy8I	GTNNAC	7 cut(s)	385, 442, 721, 840, 1006, 1042, 1674
Hpy99I	CGWCG	3 cut(s)	218, 376, 1181
HpyCH4III	ACNGT	4 cut(s)	1059, 1620, 1678, 1840
HpyCH4IV	ACGT	6 cut(s)	279, 381, 653, 1635, 1853, 1962
HpyF3I	CTNAG	5 cut(s)	550, 1082, 1143, 1525, 2000
HpySE526I	ACGT	6 cut(s)	279, 381, 653, 1635, 1853, 1962
Hsp92I	GRCGYC	4 cut(s)	60, 261, 295, 1661
Hsp92II	CATG	7 cut(s)	163, 325, 527, 691, 1253, 1369, 1660
HspAI	GCGC	6 cut(s)	120, 122, 219, 1115, 1661, 1823
KasI	GGCGCC	1 cut(s)	1660
KroI	GCCGGC	1 cut(s)	35
KroNI	GCCGGC	1 cut(s)	37
LmnI	GCTCC	4 cut(s)	811, 1075, 1644, 1930
Lsp1109I	GCAGC	8 cut(s)	6, 19, 123, 159, 208, 754, 1111, 1757
LweI	GCATC	1 cut(s)	1129
MaeI	CTAG	5 cut(s)	108, 135, 563, 1442, 1995
MaeII	ACGT	6 cut(s)	279, 381, 653, 1635, 1853, 1962
MaeIII	GTNAC	5 cut(s)	130, 346, 1474, 1734, 1858
MbiI	CCGCTC	1 cut(s)	181
MfeI	CAATTG	1 cut(s)	657
MflI	RGATCY	4 cut(s)	484, 868, 1193, 1237
MhlI	GDGCHC	6 cut(s)	341, 381, 680, 1641, 1676, 1975
MlsI	TGGCCA	1 cut(s)	954
MluCI	AATT	6 cut(s)	657, 1256, 1863, 1900, 2005, 2018
MluNI	TGGCCA	1 cut(s)	954
Mly113I	GGCGCC	1 cut(s)	1661
MlyI	GAGTC	3 cut(s)	837, 1047, 1989
MmeI	TCCRAC	1 cut(s)	894
Mox20I	TGGCCA	1 cut(s)	954
MroNI	GCCGGC	1 cut(s)	35
MroXI	GAANNNNTTC	1 cut(s)	458
MscI	TGGCCA	1 cut(s)	954
MslI	CAYNNNNRTG	2 cut(s)	716, 1162
Msp20I	TGGCCA	1 cut(s)	954
MspA1I	CMGCKG	4 cut(s)	233, 554, 1687, 1748
MspCI	CTTAAG	2 cut(s)	98, 1946
MspI	CCGG	6 cut(s)	36, 50, 258, 695, 1298, 1314
MspR9I	CCNGG	1 cut(s)	258
MunI	CAATTG	1 cut(s)	657
Mva1269I	GAATGC	1 cut(s)	1579
MvnI	CGCG	6 cut(s)	122, 438, 848, 908, 1832, 1969
NaeI	GCCGGC	1 cut(s)	37
NarI	GGCGCC	1 cut(s)	1661
NciI	CCSGG	1 cut(s)	258
NcoI	CCATGG	1 cut(s)	159
NdeI	CATATG	1 cut(s)	1201
NgoMIV	GCCGGC	1 cut(s)	35
NlaIII	CATG	7 cut(s)	163, 325, 527, 691, 1253, 1369, 1660
NlaIV	GGNNCC	5 cut(s)	43, 144, 1088, 1662, 1780
NmeAIII	GCCGAG	1 cut(s)	1061
NmuCI	GTSAC	5 cut(s)	130, 346, 1474, 1734, 1858
NruI	TCGCGA	1 cut(s)	1832
NspI	RCATGY	2 cut(s)	325, 527
NspV	TTCGAA	1 cut(s)	793
PaeR7I	CTCGAG	1 cut(s)	673
PagI	TCATGA	1 cut(s)	1249
PaqCI	CACCTGC	2 cut(s)	130, 1107
PauI	GCGCGC	1 cut(s)	120
PciI	ACATGT	2 cut(s)	321, 523
PcsI	WCGNNNNNNNCGW	3 cut(s)	23, 66, 680
PctI	GAATGC	1 cut(s)	1579
PdiI	GCCGGC	1 cut(s)	37
PdmI	GAANNNNTTC	1 cut(s)	458
PfeI	GAWTC	3 cut(s)	390, 1515, 1844
PflFI	GACNNNGTC	1 cut(s)	1599
PleI	GAGTC	3 cut(s)	836, 1046, 1988
PluTI	GGCGCC	1 cut(s)	1664
PpsI	GAGTC	3 cut(s)	836, 1046, 1988
Ppu21I	YACGTR	1 cut(s)	382
PpuMI	RGGWCCY	1 cut(s)	94
PscI	ACATGT	2 cut(s)	321, 523
Psp1406I	AACGTT	1 cut(s)	279
Psp5II	RGGWCCY	1 cut(s)	94
PspFI	CCCAGC	3 cut(s)	233, 1134, 1607
PspN4I	GGNNCC	5 cut(s)	43, 144, 1088, 1662, 1780
PspPI	GGNCC	3 cut(s)	94, 251, 662
PspPPI	RGGWCCY	1 cut(s)	94
PspXI	VCTCGAGB	1 cut(s)	673
PstNI	CAGNNNCTG	2 cut(s)	599, 1099
PsuI	RGATCY	4 cut(s)	484, 868, 1193, 1237
PsyI	GACNNNGTC	1 cut(s)	1599
PteI	GCGCGC	1 cut(s)	120
PvuII	CAGCTG	2 cut(s)	554, 1748
RruI	TCGCGA	1 cut(s)	1832
RsaI	GTAC	3 cut(s)	528, 686, 737
RsaNI	GTAC	3 cut(s)	527, 685, 736
RseI	CAYNNNNRTG	2 cut(s)	716, 1162
SalI	GTCGAC	1 cut(s)	1004
Sau96I	GGNCC	3 cut(s)	94, 251, 662
SchI	GAGTC	3 cut(s)	837, 1047, 1989
ScrFI	CCNGG	1 cut(s)	258
SduI	GDGCHC	6 cut(s)	341, 381, 680, 1641, 1676, 1975
SfaNI	GCATC	1 cut(s)	1129
SfoI	GGCGCC	1 cut(s)	1662
Sfr274I	CTCGAG	1 cut(s)	673
SfuI	TTCGAA	1 cut(s)	793
SinI	GGWCC	2 cut(s)	94, 662
SlaI	CTCGAG	1 cut(s)	673
SmiMI	CAYNNNNRTG	2 cut(s)	716, 1162
SmlI	CTYRAG	3 cut(s)	98, 673, 1946
SmoI	CTYRAG	3 cut(s)	98, 673, 1946
Sse9I	AATT	6 cut(s)	657, 1256, 1863, 1900, 2005, 2018
SspDI	GGCGCC	1 cut(s)	1660
SspMI	CTAG	5 cut(s)	108, 135, 563, 1442, 1995
StyD4I	CCNGG	1 cut(s)	256
StyI	CCWWGG	2 cut(s)	159, 814
TaaI	ACNGT	4 cut(s)	1059, 1620, 1678, 1840
TaiI	ACGT	6 cut(s)	282, 384, 656, 1638, 1856, 1965
TasI	AATT	6 cut(s)	657, 1256, 1863, 1900, 2005, 2018
TatI	WGTACW	2 cut(s)	526, 735
TauI	GCSGC	4 cut(s)	11, 42, 65, 773
TfiI	GAWTC	3 cut(s)	390, 1515, 1844
TscAI	CASTG	6 cut(s)	400, 685, 1362, 1533, 1743, 1863
TseFI	GTSAC	5 cut(s)	130, 346, 1474, 1734, 1858
TseI	GCWGC	8 cut(s)	19, 32, 111, 172, 221, 767, 1124, 1745
Tsp45I	GTSAC	5 cut(s)	130, 346, 1474, 1734, 1858
TspGWI	ACGGA	5 cut(s)	654, 821, 1350, 1631, 1819
TspRI	CASTG	6 cut(s)	400, 685, 1362, 1533, 1743, 1863
Tth111I	GACNNNGTC	1 cut(s)	1599
Vha464I	CTTAAG	2 cut(s)	98, 1946
VneI	GTGCAC	1 cut(s)	1672
VpaK11BI	GGWCC	2 cut(s)	94, 662
XagI	CCTNNNNNAGG	1 cut(s)	813
XapI	RAATTY	3 cut(s)	1256, 1900, 2005
XceI	RCATGY	2 cut(s)	325, 527
XcmI	CCANNNNNNNNNTGG	1 cut(s)	656
XhoI	CTCGAG	1 cut(s)	673
XmiI	GTMKAC	4 cut(s)	384, 441, 1005, 1041
XmnI	GAANNNNTTC	1 cut(s)	458
XspI	CTAG	5 cut(s)	108, 135, 563, 1442, 1995

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.