Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0001310

Physical Location & Seq

Genomic Coordinates Reverse (-)

50761531 .. 50765311

Gene Structure 3781 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G024830.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2154 bp

ATGCGCATCCTCACTTTCCTAACCTCATCGATACCAATTGGCCACACTTTCTTCACTCGTCTCACTCGCTCCTCATCGTCTCTACCTCTCAGCTTCAGAACCACCACCATGGCCGCTTCTTCCTCCTCCGAGGACTCCCTCCGCAAAGCCCTCTCCGAGAAGCTCTCGGAGGTCGAGAGCCAGGGCAACCAGGTCCGTTCTCTGAAGAAATCCGGCGCCGCCAAGACGGAACTTGACGCCGCCATTGAGGCCTTGAACGCGCTCAAGCTTGAGAAGGCGTCGATCGAGAAAGACCTCCAAGCCACCATCAGCGGCGACGACGGCGGCTCCGTTAACAAGGAGGCGTTACGTCAGGCTGTGGTGAATACTCTGGAGCGCCGTTTGTTTTATATTCCCGCTTTCAAGATTTACGGCGGCGTTGCTGGCCTCTACGACTACGGGCCTCCTGGTTGCGCCGTCAAATCCAATGTCCTAGGGTTTTGGCGTCAGCATTTTGTGCTTGAAGAGAACATGTTGGAAGTTGACTGCCCTTGTGTCACACCGGAGGTGGTTTTGAAAGCATCGGGTCATGTGGAGAAATTCACCGATCTTATGGTTAAGGATGAGGCGTCTGGGATGTGCTACAGAGCTGATCACTTGCTCAAGGATTACTGTAAAGATAAGCTTGAAAAGGACCTCAGAATTACTTCAGAGATGGCAATGGAACTGAAAGAAGTACTTTCCAAATTAGATGTCCTCTCCGCTGAAGAGCTGGGTGCATGTATAAAGAAATATGGTATTACAGCCCCTGACACAAAGAACACTCTCTCGGATCCTTATCCATTCAATCTGATGTTTCAAACATCAATTGGTCCTTCTGGTGTGAGCACCGGGTATATGCGTCCAGAAACTGCCCAAGGCATATTTGTGAATTTTAGGGACTTGTACTATTACAATGGGAACAAACTCCCTTTTGCTGCAGCCCAAATTGGCCAGGCTTTTAGAAATGAGATATCACCTCGCCAAGGTCTTTTGAGAGTCCGTGAGTTTACACTTGCAGAGATTGAGCACTTTGTTGACCCCGAAGACAAATCTCACCCAAAATTCTCTGATGTTGCACATTTGGAGTTTCTGATGTTCCCAAGGGATGAACAGAAGCCTGGCCAGTCTGCAAGGATAATCTCACTAGGTGAAGCTGTATCTTCTAAGGTTGTATACCTCTTCCTTACTCATCTGGGTATAGACAAGAACCGTTTAAGGTTCCGGCAGCACCTTGCAAATGAAATGGCTCACTATGCCCAGGACTGTTGGGATGCTGAGATTGAGACTTCATATGGCTGGATTGAGTGCGTGGGTATTGCAGATAGATCTGCGTATGACTTGAAGGCTCATTCGGAGAAAAGTAGAGTTGATCTTGAGGCACAGGAAAAATATTCCGAACCAAGAGAAGTTGAGAAATTGGTTATATCTCCTGTAAAGAAAGCGATTGGTCTTGCTTTCAAGGGGGACCAAAAGAAAGTACTTGAAGCTTTGGAGGCCATGAAAGAAGAAGAAGCTTTGAAAATGAAGACTGATTTAGAGTCAAAAGGGGAGGTAGAGTTTTATGTCTGCACTCTAGAGAAGAAAGTATCTATTAATTCAAGTATGGTAACAATTTCAAAGGAGAAAAAGAAAGAACACCAGAGAGTATTCACGCCCTCGGTAATTGAGCCTTCTTTTGGCATCGGTCGGATAATATATTGCCTCTATGAGCACTCATACTACATGAGGCCAAGTAAAGATGGGAATGAACAGTTTAATGTGTTCCGTTTTCCCCCTCTTGTGGCACCCATCAAGTGCACAGTTTTCCCGTTGGTTCAGAAGCAAGAGTATGAGGAAGTTTCTAAAGTCATTTCCAAATCACTGACTGCATCTGGGATATCACATAAAATTGACATCACAGGTACCTCAATTGGGAAACGATATGCACGGACTGATGAACTGGGTGTACCGTTTGCAATCACAGTTGATTCCGTATCTTCTGTGACAATCCGGGAACGAGACAGCAAGGATCAGATCCGGGTTGATGTGAGTGAGGCAGCGTCTGTTGTCAAGGATATAGCTGAGGGAGTGAGGACATGGGCGGATGTGTGGTCGGCTTTCCCACACCATTCTTCTGGCTCAGCTGACGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0003674 GO:0003824 GO:0004812 GO:0004820 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006426 GO:0006518 GO:0006520 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009117 GO:0009165 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0012505 GO:0015959 GO:0015960 GO:0015965 GO:0015966 GO:0016070 GO:0016874 GO:0016875 GO:0018130 GO:0019438 GO:0019538 GO:0019637 GO:0019752 GO:0031410 GO:0031982 GO:0031984 GO:0032543 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046686 GO:0046983 GO:0050896 GO:0055086 GO:0070127 GO:0070150 GO:0071704 GO:0090304 GO:0090407 GO:0097708 GO:0098791 GO:0140053 GO:0140098 GO:0140101 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon WHEP-TRS tRNA-synt_2b

Protein Analysis

717

Amino Acids

80.2

Weight (kDa)

6.07

Isoelectric Point (pI)

39.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHEP-TRS	PF00458	56 - 99	2.8e-09	WHEP-TRS domain
tRNA-synt_2b	PF00587	292 - 578	4.3e-10	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	605 - 692	1.6e-17	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29880
fragaria_vesca	FvH4_4g22441 FvH4_4g22450 FvH4_4g22450 FvH4_4g22610 FvH4_4g22610
malus_domestica	MD13G1131000.v1.1 MD13G1145800.v1.1 MD16G1141200.v1.1
prunus_persica	Prupe.1G214600_v2.0.a1
pyrus_communis	pycom13g11430 pycom13g12530 pycom16g11160 pycom16g11170
rosa_chinensis	RchiOBHm_Chr1g0361881 RchiOBHm_Chr4g0428711 RchiOBHm_Chr4g0428851
rosa_laevigata	RLG00000007120 RLG00000007133 RLG00000027689 RLG00000027690 RLG00000027694
rosa_multiflora	Rmu_co8208336.1_g000001 Rmu_co8210234.1_g000001 Rmu_sc0003316.1_g000006 Rmu_sc0008612.1_g000003 Rmu_sc0018216.1_g000001 Rmu_ssc0000124.1_g000013
rosa_roxburghii	Rroxscaffold_4G00294630 Rroxscaffold_5G00370530
rosa_rugosa	Rorug01G0292100 Rorug04G0227400 Rorug04G0227500 Rorug04G0229700
rosa_samantha	Rh1AG302800 Rh1BG265900 Rh1BG266000 Rh1CG284000 Rh1DG295900 Rh1DG296000 Rh4AG284700 Rh4AG284900 Rh4AG286400 Rh4BG290700 Rh4BG290900 Rh4BG292200 Rh4CG308200 Rh4DG288200 Rh4DG289400
rosa_wichuraiana	Rw0G020620 Rw1G026830 Rw4G024720 Rw4G024830 Rw7G040280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	5
Acc65I	GGTACC	1 cut(s)	1922
AccB1I	GGYRCC	3 cut(s)	215, 1804, 1922
AccI	GTMKAC	1 cut(s)	1194
AccII	CGCG	1 cut(s)	260
AclWI	GGATC	4 cut(s)	806, 819, 2029, 2037
AcoI	YGGCCR	4 cut(s)	40, 111, 970, 1141
AcsI	RAATTY	3 cut(s)	578, 910, 1082
AcuI	CTGAAG	4 cut(s)	79, 224, 672, 765
AcyI	GRCGYC	5 cut(s)	216, 237, 278, 484, 608
AdeI	CACNNNGTG	1 cut(s)	1169
AfaI	GTAC	5 cut(s)	717, 926, 1500, 1924, 1968
AfiI	CCNNNNNNNGG	4 cut(s)	1004, 1697, 1801, 1932
AflIII	ACRYGT	1 cut(s)	510
AjnI	CCWGG	6 cut(s)	180, 189, 445, 972, 1138, 1278
Alw21I	GWGCWC	4 cut(s)	869, 1050, 1734, 1820
Alw26I	GTCTC	4 cut(s)	65, 84, 1298, 2013
Alw44I	GTGCAC	1 cut(s)	1816
AlwI	GGATC	4 cut(s)	806, 819, 2029, 2037
AlwNI	CAGNNNCTG	3 cut(s)	788, 890, 2063
AoxI	GGCC	9 cut(s)	40, 111, 249, 424, 440, 970, 1141, 1515, 1748
ApaLI	GTGCAC	1 cut(s)	1816
ApeKI	GCWGC	4 cut(s)	956, 959, 1246, 2057
ApoI	RAATTY	3 cut(s)	578, 910, 1082
ArsI	GACNNNNNNTTYG	4 cut(s)	453, 485, 717, 749
AseI	ATTAAT	1 cut(s)	1614
Asp700I	GAANNNNTTC	1 cut(s)	685
Asp718I	GGTACC	1 cut(s)	1922
AspA2I	CCTAGG	1 cut(s)	472
AspLEI	GCGC	5 cut(s)	6, 218, 262, 378, 455
AspS9I	GGNCC	5 cut(s)	193, 440, 673, 851, 1486
AsuC2I	CCSGG	3 cut(s)	871, 2012, 2039
AsuHPI	GGTGA	5 cut(s)	373, 574, 987, 1067, 1181
AvaII	GGWCC	4 cut(s)	193, 673, 851, 1486
AvrII	CCTAGG	1 cut(s)	472
BaeGI	GKGCMC	1 cut(s)	1820
BalI	TGGCCA	3 cut(s)	42, 972, 1143
BamHI	GGATCC	1 cut(s)	811
BanI	GGYRCC	3 cut(s)	215, 1804, 1922
BbsI	GAAGAC	2 cut(s)	1071, 1553
Bbv12I	GWGCWC	4 cut(s)	869, 1050, 1734, 1820
BbvCI	CCTCAGC	1 cut(s)	2082
BbvI	GCAGC	4 cut(s)	943, 971, 1258, 2069
BccI	CCATC	4 cut(s)	314, 688, 1754, 1817
BceAI	ACGGC	4 cut(s)	337, 363, 427, 440
BciT130I	CCWGG	6 cut(s)	182, 191, 447, 974, 1140, 1280
BclI	TGATCA	1 cut(s)	631
BcnI	CCSGG	3 cut(s)	871, 2012, 2039
BcoDI	GTCTC	4 cut(s)	65, 84, 1298, 2013
BfaI	CTAG	3 cut(s)	473, 1166, 1595
BfmI	CTRYAG	2 cut(s)	622, 957
BfoI	RGCGCY	2 cut(s)	219, 379
BglI	GCCNNNNNGGC	1 cut(s)	248
BglII	AGATCT	1 cut(s)	1346
BlnI	CCTAGG	1 cut(s)	472
BlpI	GCTNAGC	1 cut(s)	2140
BmcAI	AGTACT	2 cut(s)	717, 1500
Bme1390I	CCNGG	9 cut(s)	182, 191, 447, 871, 974, 1140, 1280, 2012, 2039
Bme18I	GGWCC	4 cut(s)	193, 673, 851, 1486
BmgT120I	GGNCC	5 cut(s)	193, 440, 673, 851, 1486
BmiI	GGNNCC	7 cut(s)	217, 328, 813, 1241, 1487, 1806, 1924
BmrFI	CCNGG	9 cut(s)	182, 191, 447, 871, 974, 1140, 1280, 2012, 2039
BmrI	ACTGGG	1 cut(s)	1970
BmsI	GCATC	5 cut(s)	15, 569, 1282, 1710, 1898
BmuI	ACTGGG	1 cut(s)	1970
BpiI	GAAGAC	2 cut(s)	1071, 1553
BplI	GAGNNNNNCTC	2 cut(s)	149, 181
BpmI	CTGGAG	1 cut(s)	392
Bpu10I	CCTNAGC	1 cut(s)	2082
Bpu1102I	GCTNAGC	1 cut(s)	2140
BpuEI	CTTGAG	4 cut(s)	248, 290, 626, 1415
BpuMI	CCSGG	3 cut(s)	871, 2012, 2039
Bsa29I	ATCGAT	1 cut(s)	29
BsaBI	GATNNNNATC	1 cut(s)	816
BsaHI	GRCGYC	5 cut(s)	216, 237, 278, 484, 608
BsaJI	CCNNGG	9 cut(s)	108, 129, 181, 472, 895, 1003, 1121, 1278, 1677
BsaWI	WCCGGW	1 cut(s)	541
BsaXI	ACNNNNNCTCC	4 cut(s)	311, 341, 1562, 1592
Bsc4I	CCNNNNNNNGG	4 cut(s)	1004, 1697, 1801, 1932
Bse1I	ACTGG	2 cut(s)	1144, 1965
Bse3DI	GCAATG	1 cut(s)	705
Bse8I	GATNNNNATC	1 cut(s)	816
BseBI	CCWGG	6 cut(s)	182, 191, 447, 974, 1140, 1280
BseCI	ATCGAT	1 cut(s)	29
BseDI	CCNNGG	9 cut(s)	108, 129, 181, 472, 895, 1003, 1121, 1278, 1677
BseGI	GGATG	6 cut(s)	6, 607, 621, 1132, 1297, 2110
BseJI	GATNNNNATC	1 cut(s)	816
BseLI	CCNNNNNNNGG	4 cut(s)	1004, 1697, 1801, 1932
BseMI	GCAATG	1 cut(s)	705
BseMII	CTCAG	5 cut(s)	103, 691, 1287, 2073, 2154
BseNI	ACTGG	2 cut(s)	1144, 1965
BseRI	GAGGAG	2 cut(s)	61, 115
BseSI	GKGCMC	1 cut(s)	1820
BseXI	GCAGC	4 cut(s)	943, 971, 1258, 2069
BseYI	CCCAGC	1 cut(s)	751
BsgI	GTGCAG	1 cut(s)	1573
Bsh1236I	CGCG	1 cut(s)	260
Bsh1285I	CGRYCG	2 cut(s)	285, 1708
BshFI	GGCC	9 cut(s)	42, 113, 251, 426, 442, 972, 1143, 1517, 1750
BshNI	GGYRCC	3 cut(s)	215, 1804, 1922
BshVI	ATCGAT	1 cut(s)	29
BsiEI	CGRYCG	2 cut(s)	285, 1708
BsiHKAI	GWGCWC	4 cut(s)	869, 1050, 1734, 1820
BsiSI	CCGG	6 cut(s)	213, 542, 870, 1243, 2011, 2038
BslFI	GGGAC	2 cut(s)	932, 1499
BslI	CCNNNNNNNGG	4 cut(s)	1004, 1697, 1801, 1932
BsmAI	GTCTC	4 cut(s)	65, 84, 1298, 2013
BsmBI	CGTCTC	2 cut(s)	65, 84
BsmFI	GGGAC	2 cut(s)	932, 1499
BsnI	GGCC	9 cut(s)	42, 113, 251, 426, 442, 972, 1143, 1517, 1750
Bsp1286I	GDGCHC	4 cut(s)	869, 1050, 1734, 1820
Bsp143I	GATC	8 cut(s)	282, 586, 631, 811, 1346, 1390, 2029, 2034
Bsp1720I	GCTNAGC	1 cut(s)	2140
Bsp19I	CCATGG	1 cut(s)	108
BspANI	GGCC	9 cut(s)	42, 113, 251, 426, 442, 972, 1143, 1517, 1750
BspCNI	CTCAG	5 cut(s)	102, 690, 1288, 2074, 2153
BspDI	ATCGAT	1 cut(s)	29
BspFNI	CGCG	1 cut(s)	260
BspLI	GGNNCC	7 cut(s)	217, 328, 813, 1241, 1487, 1806, 1924
BspMAI	CTGCAG	1 cut(s)	961
BspPI	GGATC	4 cut(s)	806, 819, 2029, 2037
BspQI	GCTCTTC	1 cut(s)	741
BspT107I	GGYRCC	3 cut(s)	215, 1804, 1922
BsrDI	GCAATG	1 cut(s)	705
BsrI	ACTGG	2 cut(s)	1144, 1965
BssECI	CCNNGG	9 cut(s)	108, 129, 181, 472, 895, 1003, 1121, 1278, 1677
BssMI	GATC	8 cut(s)	282, 586, 631, 811, 1346, 1390, 2029, 2034
BssNAI	GTATAC	1 cut(s)	1195
BssNI	GRCGYC	5 cut(s)	216, 237, 278, 484, 608
BssT1I	CCWWGG	5 cut(s)	108, 472, 895, 1003, 1121
Bst1107I	GTATAC	1 cut(s)	1195
Bst2UI	CCWGG	6 cut(s)	182, 191, 447, 974, 1140, 1280
Bst4CI	ACNGT	7 cut(s)	653, 1232, 1286, 1773, 1822, 1971, 1984
Bst6I	CTCTTC	3 cut(s)	498, 741, 1205
BstACI	GRCGYC	5 cut(s)	216, 237, 278, 484, 608
BstAPI	GCANNNNNTGC	1 cut(s)	496
BstC8I	GCNNGC	1 cut(s)	424
BstDEI	CTNAG	6 cut(s)	89, 677, 1185, 1296, 2082, 2140
BstDSI	CCRYGG	1 cut(s)	108
BstENI	CCTNNNNNAGG	1 cut(s)	1002
BstF5I	GGATG	6 cut(s)	6, 607, 621, 1132, 1297, 2110
BstFNI	CGCG	1 cut(s)	260
BstH2I	RGCGCY	2 cut(s)	219, 379
BstHHI	GCGC	5 cut(s)	6, 218, 262, 378, 455
BstKTI	GATC	8 cut(s)	285, 589, 634, 814, 1349, 1393, 2032, 2037
BstMAI	GTCTC	4 cut(s)	65, 84, 1298, 2013
BstMBI	GATC	8 cut(s)	282, 586, 631, 811, 1346, 1390, 2029, 2034
BstMCI	CGRYCG	2 cut(s)	285, 1708
BstMWI	GCNNNNNNNGC	7 cut(s)	248, 257, 321, 423, 496, 1274, 1514
BstNI	CCWGG	6 cut(s)	182, 191, 447, 974, 1140, 1280
BstNSI	RCATGY	2 cut(s)	514, 762
BstSCI	CCNGG	9 cut(s)	180, 189, 445, 869, 972, 1138, 1278, 2010, 2037
BstSFI	CTRYAG	2 cut(s)	622, 957
BstSLI	GKGCMC	1 cut(s)	1820
BstUI	CGCG	1 cut(s)	260
BstV1I	GCAGC	4 cut(s)	943, 971, 1258, 2069
BstV2I	GAAGAC	2 cut(s)	1071, 1553
BstX2I	RGATCY	3 cut(s)	811, 1346, 2034
BstXI	CCANNNNNNTGG	2 cut(s)	109, 2135
BstYI	RGATCY	3 cut(s)	811, 1346, 2034
BstZ17I	GTATAC	1 cut(s)	1195
Bsu15I	ATCGAT	1 cut(s)	29
BsuRI	GGCC	9 cut(s)	42, 113, 251, 426, 442, 972, 1143, 1517, 1750
BsuTUI	ATCGAT	1 cut(s)	29
BtgI	CCRYGG	1 cut(s)	108
BtsCI	GGATG	6 cut(s)	6, 607, 621, 1132, 1297, 2110
BtsIMutI	CAGTG	1 cut(s)	1880
Cac8I	GCNNGC	1 cut(s)	424
CaiI	CAGNNNCTG	3 cut(s)	788, 890, 2063
CfoI	GCGC	5 cut(s)	6, 218, 262, 378, 455
Cfr13I	GGNCC	5 cut(s)	193, 440, 673, 851, 1486
ClaI	ATCGAT	1 cut(s)	29
CseI	GACGC	6 cut(s)	245, 267, 473, 597, 869, 2049
CsiI	ACCWGGT	1 cut(s)	189
Csp6I	GTAC	5 cut(s)	716, 925, 1499, 1923, 1967
CviAII	CATG	7 cut(s)	109, 511, 569, 759, 1519, 1744, 2097
CviQI	GTAC	5 cut(s)	716, 925, 1499, 1923, 1967
DdeI	CTNAG	6 cut(s)	89, 677, 1185, 1296, 2082, 2140
DinI	GGCGCC	1 cut(s)	217
DpnI	GATC	8 cut(s)	284, 588, 633, 813, 1348, 1392, 2031, 2036
DpnII	GATC	8 cut(s)	282, 586, 631, 811, 1346, 1390, 2029, 2034
DraIII	CACNNNGTG	1 cut(s)	1169
EaeI	YGGCCR	4 cut(s)	40, 111, 970, 1141
Eam1104I	CTCTTC	3 cut(s)	498, 741, 1205
EarI	CTCTTC	3 cut(s)	498, 741, 1205
EciI	GGCGGA	1 cut(s)	2117
Eco130I	CCWWGG	5 cut(s)	108, 472, 895, 1003, 1121
Eco147I	AGGCCT	1 cut(s)	251
Eco32I	GATATC	2 cut(s)	993, 1899
Eco47I	GGWCC	4 cut(s)	193, 673, 851, 1486
Eco57I	CTGAAG	4 cut(s)	79, 224, 672, 765
EcoNI	CCTNNNNNAGG	1 cut(s)	1002
EcoO109I	RGGNCCY	1 cut(s)	673
EcoRII	CCWGG	6 cut(s)	180, 189, 445, 972, 1138, 1278
EcoRV	GATATC	2 cut(s)	993, 1899
EcoT14I	CCWWGG	5 cut(s)	108, 472, 895, 1003, 1121
EgeI	GGCGCC	1 cut(s)	217
EheI	GGCGCC	1 cut(s)	217
ErhI	CCWWGG	5 cut(s)	108, 472, 895, 1003, 1121
Esp3I	CGTCTC	2 cut(s)	65, 84
FaeI	CATG	7 cut(s)	112, 514, 572, 762, 1522, 1747, 2100
FalI	AAGNNNNNCTT	4 cut(s)	648, 680, 702, 734
FaqI	GGGAC	2 cut(s)	932, 1499
FatI	CATG	7 cut(s)	108, 510, 568, 758, 1518, 1743, 2096
FauI	CCCGC	1 cut(s)	403
FauNDI	CATATG	1 cut(s)	1312
FbaI	TGATCA	1 cut(s)	631
FblI	GTMKAC	1 cut(s)	1194
FokI	GGATG	5 cut(s)	614, 628, 1139, 1304, 2117
FspAI	RTGCGCAY	1 cut(s)	5
FspBI	CTAG	3 cut(s)	473, 1166, 1595
FspI	TGCGCA	1 cut(s)	5
GlaI	GCGC	5 cut(s)	5, 217, 261, 377, 454
GsaI	CCCAGC	1 cut(s)	755
GsuI	CTGGAG	1 cut(s)	392
HaeII	RGCGCY	2 cut(s)	219, 379
HaeIII	GGCC	9 cut(s)	42, 113, 251, 426, 442, 972, 1143, 1517, 1750
HapII	CCGG	6 cut(s)	213, 542, 870, 1243, 2011, 2038
HgaI	GACGC	6 cut(s)	245, 267, 473, 597, 869, 2049
HhaI	GCGC	5 cut(s)	6, 218, 262, 378, 455
Hin1I	GRCGYC	5 cut(s)	216, 237, 278, 484, 608
Hin1II	CATG	7 cut(s)	112, 514, 572, 762, 1522, 1747, 2100
Hin6I	GCGC	5 cut(s)	4, 216, 260, 376, 453
HinP1I	GCGC	5 cut(s)	4, 216, 260, 376, 453
HincII	GTYRAC	3 cut(s)	334, 523, 1057
HindII	GTYRAC	3 cut(s)	334, 523, 1057
HindIII	AAGCTT	4 cut(s)	266, 662, 1506, 1533
HinfI	GANTC	4 cut(s)	134, 1017, 1559, 1988
HpaI	GTTAAC	1 cut(s)	334
HpaII	CCGG	6 cut(s)	213, 542, 870, 1243, 2011, 2038
HphI	GGTGA	5 cut(s)	373, 574, 987, 1067, 1181
Hpy166II	GTNNAC	7 cut(s)	334, 523, 1029, 1057, 1195, 1818, 1967
Hpy188III	TCNNGA	7 cut(s)	175, 286, 371, 403, 884, 1394, 1595
Hpy8I	GTNNAC	7 cut(s)	334, 523, 1029, 1057, 1195, 1818, 1967
Hpy99I	CGWCG	3 cut(s)	283, 320, 323
HpyAV	CCTTC	4 cut(s)	268, 864, 1357, 1701
HpyCH4III	ACNGT	7 cut(s)	653, 1232, 1286, 1773, 1822, 1971, 1984
HpyCH4IV	ACGT	1 cut(s)	349
HpyF10VI	GCNNNNNNNGC	7 cut(s)	248, 257, 321, 423, 496, 1274, 1514
HpyF3I	CTNAG	6 cut(s)	89, 677, 1185, 1296, 2082, 2140
HpySE526I	ACGT	1 cut(s)	349
Hsp92I	GRCGYC	5 cut(s)	216, 237, 278, 484, 608
Hsp92II	CATG	7 cut(s)	112, 514, 572, 762, 1522, 1747, 2100
HspAI	GCGC	5 cut(s)	4, 216, 260, 376, 453
KasI	GGCGCC	1 cut(s)	215
KpnI	GGTACC	1 cut(s)	1926
Ksp22I	TGATCA	1 cut(s)	631
KspAI	GTTAAC	1 cut(s)	334
Kzo9I	GATC	8 cut(s)	282, 586, 631, 811, 1346, 1390, 2029, 2034
LguI	GCTCTTC	1 cut(s)	741
LmnI	GCTCC	3 cut(s)	74, 332, 373
Lsp1109I	GCAGC	4 cut(s)	943, 971, 1258, 2069
LweI	GCATC	5 cut(s)	15, 569, 1282, 1710, 1898
MabI	ACCWGGT	1 cut(s)	189
MaeI	CTAG	3 cut(s)	473, 1166, 1595
MaeII	ACGT	1 cut(s)	349
MaeIII	GTNAC	4 cut(s)	345, 535, 1627, 2002
MalI	GATC	8 cut(s)	284, 588, 633, 813, 1348, 1392, 2031, 2036
MboI	GATC	8 cut(s)	282, 586, 631, 811, 1346, 1390, 2029, 2034
MfeI	CAATTG	3 cut(s)	36, 846, 1929
MflI	RGATCY	3 cut(s)	811, 1346, 2034
MhlI	GDGCHC	4 cut(s)	869, 1050, 1734, 1820
MlsI	TGGCCA	3 cut(s)	42, 972, 1143
MluNI	TGGCCA	3 cut(s)	42, 972, 1143
Mly113I	GGCGCC	1 cut(s)	216
MlyI	GAGTC	3 cut(s)	128, 1026, 1568
MmeI	TCCRAC	2 cut(s)	495, 1688
Mox20I	TGGCCA	3 cut(s)	42, 972, 1143
MroXI	GAANNNNTTC	1 cut(s)	685
MscI	TGGCCA	3 cut(s)	42, 972, 1143
MseI	TTAA	5 cut(s)	333, 597, 1235, 1614, 1776
MslI	CAYNNNNRTG	2 cut(s)	107, 905
Msp20I	TGGCCA	3 cut(s)	42, 972, 1143
MspA1I	CMGCKG	3 cut(s)	312, 743, 2144
MspI	CCGG	6 cut(s)	213, 542, 870, 1243, 2011, 2038
MspR9I	CCNGG	9 cut(s)	182, 191, 447, 871, 974, 1140, 1280, 2012, 2039
MunI	CAATTG	3 cut(s)	36, 846, 1929
MvaI	CCWGG	6 cut(s)	182, 191, 447, 974, 1140, 1280
MvnI	CGCG	1 cut(s)	260
MwoI	GCNNNNNNNGC	7 cut(s)	248, 257, 321, 423, 496, 1274, 1514
NarI	GGCGCC	1 cut(s)	216
NciI	CCSGG	3 cut(s)	871, 2012, 2039
NcoI	CCATGG	1 cut(s)	108
NdeI	CATATG	1 cut(s)	1312
NdeII	GATC	8 cut(s)	282, 586, 631, 811, 1346, 1390, 2029, 2034
NlaIII	CATG	7 cut(s)	112, 514, 572, 762, 1522, 1747, 2100
NlaIV	GGNNCC	7 cut(s)	217, 328, 813, 1241, 1487, 1806, 1924
NmuCI	GTSAC	2 cut(s)	535, 2002
NsbI	TGCGCA	1 cut(s)	5
NspI	RCATGY	2 cut(s)	514, 762
PceI	AGGCCT	1 cut(s)	251
PciI	ACATGT	1 cut(s)	510
PciSI	GCTCTTC	1 cut(s)	741
PcsI	WCGNNNNNNNCGW	1 cut(s)	327
PdmI	GAANNNNTTC	1 cut(s)	685
PfeI	GAWTC	1 cut(s)	1988
PfoI	TCCNGGA	1 cut(s)	2010
Ple19I	CGATCG	1 cut(s)	285
PleI	GAGTC	3 cut(s)	128, 1025, 1567
PluTI	GGCGCC	1 cut(s)	219
PpsI	GAGTC	3 cut(s)	128, 1025, 1567
PpuMI	RGGWCCY	1 cut(s)	673
PscI	ACATGT	1 cut(s)	510
PshBI	ATTAAT	1 cut(s)	1614
Psp5II	RGGWCCY	1 cut(s)	673
Psp6I	CCWGG	6 cut(s)	180, 189, 445, 972, 1138, 1278
PspFI	CCCAGC	1 cut(s)	751
PspGI	CCWGG	6 cut(s)	180, 189, 445, 972, 1138, 1278
PspN4I	GGNNCC	7 cut(s)	217, 328, 813, 1241, 1487, 1806, 1924
PspPI	GGNCC	5 cut(s)	193, 440, 673, 851, 1486
PspPPI	RGGWCCY	1 cut(s)	673
PsrI	GAACNNNNNNTAC	2 cut(s)	1950, 1982
PstI	CTGCAG	1 cut(s)	961
PstNI	CAGNNNCTG	3 cut(s)	788, 890, 2063
PsuI	RGATCY	3 cut(s)	811, 1346, 2034
PvuI	CGATCG	1 cut(s)	285
PvuII	CAGCTG	1 cut(s)	2144
RsaI	GTAC	5 cut(s)	717, 926, 1500, 1924, 1968
RsaNI	GTAC	5 cut(s)	716, 925, 1499, 1923, 1967
RseI	CAYNNNNRTG	2 cut(s)	107, 905
SapI	GCTCTTC	1 cut(s)	741
SaqAI	TTAA	5 cut(s)	333, 597, 1235, 1614, 1776
Sau3AI	GATC	8 cut(s)	282, 586, 631, 811, 1346, 1390, 2029, 2034
Sau96I	GGNCC	5 cut(s)	193, 440, 673, 851, 1486
ScaI	AGTACT	2 cut(s)	717, 1500
SchI	GAGTC	3 cut(s)	128, 1026, 1568
ScrFI	CCNGG	9 cut(s)	182, 191, 447, 871, 974, 1140, 1280, 2012, 2039
SduI	GDGCHC	4 cut(s)	869, 1050, 1734, 1820
SexAI	ACCWGGT	1 cut(s)	189
SfaNI	GCATC	5 cut(s)	15, 569, 1282, 1710, 1898
SfcI	CTRYAG	2 cut(s)	622, 957
SfoI	GGCGCC	1 cut(s)	217
SinI	GGWCC	4 cut(s)	193, 673, 851, 1486
SmiMI	CAYNNNNRTG	2 cut(s)	107, 905
SmlI	CTYRAG	4 cut(s)	263, 269, 641, 1394
SmoI	CTYRAG	4 cut(s)	263, 269, 641, 1394
SseBI	AGGCCT	1 cut(s)	251
SspDI	GGCGCC	1 cut(s)	215
SspI	AATATT	1 cut(s)	1412
SspMI	CTAG	3 cut(s)	473, 1166, 1595
StuI	AGGCCT	1 cut(s)	251
StyD4I	CCNGG	9 cut(s)	180, 189, 445, 869, 972, 1138, 1278, 2010, 2037
StyI	CCWWGG	5 cut(s)	108, 472, 895, 1003, 1121
TaaI	ACNGT	7 cut(s)	653, 1232, 1286, 1773, 1822, 1971, 1984
TaiI	ACGT	1 cut(s)	352
TaqI	TCGA	4 cut(s)	29, 174, 281, 285
TaqII	GACCGA	1 cut(s)	1694
TatI	WGTACW	3 cut(s)	715, 924, 1498
TauI	GCSGC	6 cut(s)	116, 221, 242, 315, 327, 417
TfiI	GAWTC	1 cut(s)	1988
Tru1I	TTAA	5 cut(s)	333, 597, 1235, 1614, 1776
Tru9I	TTAA	5 cut(s)	333, 597, 1235, 1614, 1776
TscAI	CASTG	1 cut(s)	1887
TseFI	GTSAC	2 cut(s)	535, 2002
TseI	GCWGC	4 cut(s)	956, 959, 1246, 2057
Tsp45I	GTSAC	2 cut(s)	535, 2002
TspDTI	ATGAA	7 cut(s)	1143, 1275, 1299, 1535, 1559, 1782, 1971
TspGWI	ACGGA	7 cut(s)	185, 242, 319, 1010, 1775, 1963, 1981
TspRI	CASTG	1 cut(s)	1887
VneI	GTGCAC	1 cut(s)	1816
VpaK11BI	GGWCC	4 cut(s)	193, 673, 851, 1486
VspI	ATTAAT	1 cut(s)	1614
XagI	CCTNNNNNAGG	1 cut(s)	1002
XapI	RAATTY	3 cut(s)	578, 910, 1082
XbaI	TCTAGA	1 cut(s)	1594
XceI	RCATGY	2 cut(s)	514, 762
XmaJI	CCTAGG	1 cut(s)	472
XmiI	GTMKAC	1 cut(s)	1194
XmnI	GAANNNNTTC	1 cut(s)	685
XspI	CTAG	3 cut(s)	473, 1166, 1595
ZrmI	AGTACT	2 cut(s)	717, 1500

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.