Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0001310

Physical Location & Seq

Genomic Coordinates Forward (+)

14658768 .. 14660657

Gene Structure 1890 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00294630.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1890 bp

ATGAGTAATAATACAGAAAGCTCCCGCAGTGTTCTGGCCAACACTCTTGAACGGCGCTTCTTTTACATCCCTTCTTTCAAGATCTACGGCGCCGTTGCTGGCCTCTACGACTATGGCTCGCCCGGCTGCAAACTCAAGAAGAATGTCCTTTCGCTTTGGCATCAGCACTTTGTTCATGAAGAGAACATGTGGGAGATTGACTGCCCTTGCCTCACGCCAGAGGCTGTTTTGAAGGCATCTGGCCATGTATCTAAGTTCACTGATCTCATGGTTAAGGATGAGAAGAAAGGGACTTGCTACCGGGCTGATCACTTGCTCAAGGATTTCTGCAATAAGAAGCTTGAAGATCAGGACCATTGCATTAGTCCAGAGATGGCAATGGAGCTGAAACAAGTGCTTTCTAAATTGGATGGTCTATCAGCTGAAGAGCTGGGTGCATGCATCAAGAAATATGAGATTAAAGCCCCCGAAACAGATAATTATCTCTCAGATCCTTATCCTTTCAACTTGATGTTTCAAACATCAATTGGTCCGTCTGGTTCGAGCACTGGTTTCTTCCGCCCAGAAACTGCACAAGGCATATTTGTGAATTTTATGGACATTTACTATGACAACGGGTGCAAGCTGCCTTTTGCTGTTGCTCAAGTTGGCCAGGCTTTTCGAAACGAGATATCTCCTCGCCAAGGCCTGTTGAGAGTCCGTGAGTTCACACTCGCGGAGATTGAGCACTTTGTTGATCCTGAAGACAAATCACACGTGAAATTCCCCACCGTTGACAAGTTGCAACTTCCTATGTTCCCGAGGGATGAACAAAAGCCCGGCCAATCTGCAAGATTGATCTCTCTGGGTGAAGCAGTGCATTCTAAGATTGTGGAGAACGAAACTCTTGGCTACTTCATTGGGAGAGTATATCTCTTCCTTACTCGTCTGGGTATAGATAAGAACCGTATAAGGTTCAGGCAACACCTTGCTAAAGAGATGGCTCACTATGCTGCTGACTGCTGGGATGCTGAGATTGAGACTTCCTATGGCTGGATTGAGTGCGTCGGTATTGCAGATAGATCTGCATATGACTTGAAGGCTCATTCGGGGAAAAGTCGTCTTGAGGCACATGAACAACTTTCAGAACCAAGAGAAGTGGAGAAATTGGTTATATCTCCGGTAAAGAAAGAGATTGGCCCTGCTTTCGAGAAGAAGCAAAAGAATGTGCTTGAAGCTTTGGAGGCTATGGATGAAGAAGAAATTTCAAAAATGAAGGCCGAGTTAGACTCCAAGGGGGAGGTGAACTTCTTTGTCTGCACTCTTGGGGAACATGTATCTATTAAGAGCAGTATGGTGACAATTTCAAAGGTTAAAGCAAGAGAACATCAAAGAAAATTCACACCCTCAGTAATCGAGCCTTCATTTGGTATTGGGAGGATTATATATTGTCTCTTTGAGCATTCCTTCTACACCAGACCAGATAAAACTGGGGATGAACAATTGAATGTGTTTCGGTTTAACCCACTTGTGGCGCCTTTTAAGTGCACAGTTTTCCCTCTGGTTCGGAACCAACAGTTTGAGGATGTTGCCAAGGACATTTCCAAGTCATTGACTTCTGCTGGCATATCGCATAAAATGGATACTACAGGTACCTCGATTGGGAAACGTTATGCACGTACTGATGAGCTCGGTGTACCCTTTGCAATCACGGTTGATTCTACATCCTCAGTCACAATTCGAGAAAGAGACAGCAAAGATCAAATCCGTGTTAGTGTGAAGAAGGCAGTATCTGTTGTGAAGGAGCTCAGTGAGGGGTTGAGGACTTGGGAAGATGTGTGGTGGTCACCTAGTCAACGGTGGAAGAGATTCTGGCGTTCCTGCTTCAGATTTATATTGTCTAGAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0003674 GO:0003824 GO:0004812 GO:0004820 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006426 GO:0006518 GO:0006520 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009117 GO:0009165 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0012505 GO:0015959 GO:0015960 GO:0015965 GO:0015966 GO:0016070 GO:0016874 GO:0016875 GO:0018130 GO:0019438 GO:0019538 GO:0019637 GO:0019752 GO:0031410 GO:0031982 GO:0031984 GO:0032543 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046686 GO:0046983 GO:0050896 GO:0055086 GO:0070127 GO:0070150 GO:0071704 GO:0090304 GO:0090407 GO:0097708 GO:0098791 GO:0140053 GO:0140098 GO:0140101 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon WHEP-TRS tRNA-synt_2b

Protein Analysis

629

Amino Acids

71.81

Weight (kDa)

7.24

Isoelectric Point (pI)

43.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2b	PF00587	187 - 249	2.8e-07	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	508 - 596	6.8e-20	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29880
fragaria_vesca	FvH4_4g22441 FvH4_4g22450 FvH4_4g22450 FvH4_4g22610 FvH4_4g22610
malus_domestica	MD13G1131000.v1.1 MD13G1145800.v1.1 MD16G1141200.v1.1
prunus_persica	Prupe.1G214600_v2.0.a1
pyrus_communis	pycom13g11430 pycom13g12530 pycom16g11160 pycom16g11170
rosa_chinensis	RchiOBHm_Chr1g0361881 RchiOBHm_Chr4g0428711 RchiOBHm_Chr4g0428851
rosa_laevigata	RLG00000007120 RLG00000007133 RLG00000027689 RLG00000027690 RLG00000027694
rosa_multiflora	Rmu_co8208336.1_g000001 Rmu_co8210234.1_g000001 Rmu_sc0003316.1_g000006 Rmu_sc0008612.1_g000003 Rmu_sc0018216.1_g000001 Rmu_ssc0000124.1_g000013
rosa_roxburghii	Rroxscaffold_4G00294630 Rroxscaffold_5G00370530
rosa_rugosa	Rorug01G0292100 Rorug04G0227400 Rorug04G0227500 Rorug04G0229700
rosa_samantha	Rh1AG302800 Rh1BG265900 Rh1BG266000 Rh1CG284000 Rh1DG295900 Rh1DG296000 Rh4AG284700 Rh4AG284900 Rh4AG286400 Rh4BG290700 Rh4BG290900 Rh4BG292200 Rh4CG308200 Rh4DG288200 Rh4DG289400
rosa_wichuraiana	Rw0G020620 Rw1G026830 Rw4G024720 Rw4G024830 Rw7G040280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1631
AccB1I	GGYRCC	3 cut(s)	89, 1513, 1631
AccII	CGCG	1 cut(s)	716
AciI	CCGC	3 cut(s)	25, 559, 716
AclI	AACGTT	1 cut(s)	1648
AclWI	GGATC	2 cut(s)	485, 731
AcoI	YGGCCR	4 cut(s)	36, 241, 649, 820
AcsI	RAATTY	4 cut(s)	589, 761, 1242, 1376
AcuI	CTGAAG	3 cut(s)	444, 762, 1849
AcvI	CACGTG	1 cut(s)	757
AcyI	GRCGYC	2 cut(s)	90, 1514
AfaI	GTAC	3 cut(s)	1633, 1660, 1677
AfiI	CCNNNNNNNGG	5 cut(s)	683, 1032, 1406, 1510, 1641
AflIII	ACRYGT	3 cut(s)	186, 754, 1312
AjnI	CCWGG	1 cut(s)	651
AluBI	AGCT	9 cut(s)	21, 340, 385, 422, 430, 625, 1217, 1669, 1786
AluI	AGCT	9 cut(s)	21, 340, 385, 422, 430, 625, 1217, 1669, 1786
Alw21I	GWGCWC	5 cut(s)	548, 729, 1529, 1671, 1788
Alw26I	GTCTC	3 cut(s)	1013, 1436, 1722
Alw44I	GTGCAC	1 cut(s)	1525
AlwI	GGATC	2 cut(s)	485, 731
AlwNI	CAGNNNCTG	3 cut(s)	224, 569, 1772
Ama87I	CYCGRG	1 cut(s)	799
AoxI	GGCC	8 cut(s)	36, 100, 241, 649, 685, 820, 1177, 1257
ApaLI	GTGCAC	1 cut(s)	1525
ApeKI	GCWGC	3 cut(s)	126, 625, 992
ApoI	RAATTY	4 cut(s)	589, 761, 1242, 1376
Asp700I	GAANNNNTTC	1 cut(s)	1847
Asp718I	GGTACC	1 cut(s)	1631
AspLEI	GCGC	3 cut(s)	57, 92, 1516
AspS9I	GGNCC	3 cut(s)	352, 530, 1178
AsuC2I	CCSGG	3 cut(s)	123, 302, 819
AsuHPI	GGTGA	4 cut(s)	860, 1294, 1348, 1818
AsuII	TTCGAA	1 cut(s)	661
AvaI	CYCGRG	1 cut(s)	799
AvaII	GGWCC	2 cut(s)	352, 530
BaeGI	GKGCMC	1 cut(s)	1529
BalI	TGGCCA	3 cut(s)	38, 243, 651
BanI	GGYRCC	3 cut(s)	89, 1513, 1631
BanII	GRGCYC	2 cut(s)	1671, 1788
BbrPI	CACGTG	1 cut(s)	757
BbsI	GAAGAC	1 cut(s)	750
Bbv12I	GWGCWC	5 cut(s)	548, 729, 1529, 1671, 1788
BbvI	GCAGC	3 cut(s)	113, 612, 979
BccI	CCATC	3 cut(s)	367, 404, 973
BceAI	ACGGC	3 cut(s)	68, 77, 103
BciT130I	CCWGG	1 cut(s)	653
BciVI	GTATCC	1 cut(s)	1615
BclI	TGATCA	1 cut(s)	307
BcnI	CCSGG	3 cut(s)	123, 302, 819
BcoDI	GTCTC	3 cut(s)	1013, 1436, 1722
BfaI	CTAG	2 cut(s)	1830, 1881
BfmI	CTRYAG	1 cut(s)	1626
BfoI	RGCGCY	3 cut(s)	58, 93, 1517
BfuI	GTATCC	1 cut(s)	1615
BglII	AGATCT	2 cut(s)	81, 1061
BisI	GCNGC	3 cut(s)	127, 626, 993
BlsI	GCNGC	3 cut(s)	128, 627, 994
Bme1390I	CCNGG	4 cut(s)	123, 302, 653, 819
Bme18I	GGWCC	2 cut(s)	352, 530
BmeT110I	CYCGRG	1 cut(s)	799
BmgT120I	GGNCC	3 cut(s)	352, 530, 1178
BmiI	GGNNCC	4 cut(s)	91, 1515, 1550, 1633
BmrFI	CCNGG	4 cut(s)	123, 302, 653, 819
BmrI	ACTGGG	1 cut(s)	1479
BmsI	GCATC	4 cut(s)	169, 245, 450, 997
BmuI	ACTGGG	1 cut(s)	1479
BpiI	GAAGAC	1 cut(s)	750
BplI	GAGNNNNNCTC	4 cut(s)	897, 929, 1253, 1285
Bpu14I	TTCGAA	1 cut(s)	661
BpuEI	CTTGAG	4 cut(s)	119, 302, 627, 1124
BpuMI	CCSGG	3 cut(s)	123, 302, 819
BsaAI	YACGTR	2 cut(s)	757, 1658
BsaBI	GATNNNNATC	2 cut(s)	480, 495
BsaHI	GRCGYC	2 cut(s)	90, 1514
BsaJI	CCNNGG	4 cut(s)	682, 800, 1272, 1572
BsaWI	WCCGGW	1 cut(s)	1159
Bsc4I	CCNNNNNNNGG	5 cut(s)	683, 1032, 1406, 1510, 1641
Bse1I	ACTGG	2 cut(s)	553, 1474
Bse3DI	GCAATG	2 cut(s)	355, 384
Bse8I	GATNNNNATC	2 cut(s)	480, 495
BseBI	CCWGG	1 cut(s)	653
BseDI	CCNNGG	4 cut(s)	682, 800, 1272, 1572
BseGI	GGATG	9 cut(s)	66, 283, 415, 811, 1012, 1237, 1480, 1570, 1703
BseJI	GATNNNNATC	2 cut(s)	480, 495
BseLI	CCNNNNNNNGG	5 cut(s)	683, 1032, 1406, 1510, 1641
BseMI	GCAATG	2 cut(s)	355, 384
BseMII	CTCAG	5 cut(s)	501, 1002, 1401, 1722, 1801
BseNI	ACTGG	2 cut(s)	553, 1474
BseRI	GAGGAG	1 cut(s)	666
BseSI	GKGCMC	1 cut(s)	1529
BseXI	GCAGC	3 cut(s)	113, 612, 979
BseYI	CCCAGC	2 cut(s)	430, 1002
BsgI	GTGCAG	2 cut(s)	555, 1282
Bsh1236I	CGCG	1 cut(s)	716
BshFI	GGCC	8 cut(s)	38, 102, 243, 651, 687, 822, 1179, 1259
BshNI	GGYRCC	3 cut(s)	89, 1513, 1631
BsiHKAI	GWGCWC	5 cut(s)	548, 729, 1529, 1671, 1788
BsiHKCI	CYCGRG	1 cut(s)	799
BsiSI	CCGG	4 cut(s)	123, 301, 819, 1160
BslFI	GGGAC	1 cut(s)	304
BslI	CCNNNNNNNGG	5 cut(s)	683, 1032, 1406, 1510, 1641
BsmAI	GTCTC	3 cut(s)	1013, 1436, 1722
BsmFI	GGGAC	1 cut(s)	304
BsmI	GAATGC	2 cut(s)	859, 1441
BsnI	GGCC	8 cut(s)	38, 102, 243, 651, 687, 822, 1179, 1259
BsoBI	CYCGRG	1 cut(s)	799
Bsp119I	TTCGAA	1 cut(s)	661
Bsp1286I	GDGCHC	5 cut(s)	548, 729, 1529, 1671, 1788
Bsp143I	GATC	9 cut(s)	81, 262, 307, 346, 490, 736, 837, 1061, 1738
BspACI	CCGC	3 cut(s)	25, 559, 716
BspANI	GGCC	8 cut(s)	38, 102, 243, 651, 687, 822, 1179, 1259
BspCNI	CTCAG	5 cut(s)	500, 1003, 1400, 1721, 1800
BspFNI	CGCG	1 cut(s)	716
BspHI	TCATGA	1 cut(s)	175
BspLI	GGNNCC	4 cut(s)	91, 1515, 1550, 1633
BspPI	GGATC	2 cut(s)	485, 731
BspQI	GCTCTTC	1 cut(s)	420
BspT104I	TTCGAA	1 cut(s)	661
BspT107I	GGYRCC	3 cut(s)	89, 1513, 1631
BsrDI	GCAATG	2 cut(s)	355, 384
BsrI	ACTGG	2 cut(s)	553, 1474
BssECI	CCNNGG	4 cut(s)	682, 800, 1272, 1572
BssMI	GATC	9 cut(s)	81, 262, 307, 346, 490, 736, 837, 1061, 1738
BssNI	GRCGYC	2 cut(s)	90, 1514
BssT1I	CCWWGG	3 cut(s)	682, 1272, 1572
Bst2UI	CCWGG	1 cut(s)	653
Bst4CI	ACNGT	6 cut(s)	772, 947, 1531, 1557, 1693, 1839
Bst6I	CTCTTC	4 cut(s)	174, 420, 920, 1838
BstACI	GRCGYC	2 cut(s)	90, 1514
BstBAI	YACGTR	2 cut(s)	757, 1658
BstBI	TTCGAA	1 cut(s)	661
BstC8I	GCNNGC	5 cut(s)	100, 119, 439, 623, 1603
BstDEI	CTNAG	7 cut(s)	252, 487, 864, 1011, 1387, 1708, 1787
BstEII	GGTNACC	1 cut(s)	1824
BstENI	CCTNNNNNAGG	1 cut(s)	681
BstF5I	GGATG	9 cut(s)	66, 283, 415, 811, 1012, 1237, 1480, 1570, 1703
BstFNI	CGCG	1 cut(s)	716
BstH2I	RGCGCY	3 cut(s)	58, 93, 1517
BstHHI	GCGC	3 cut(s)	57, 92, 1516
BstKTI	GATC	9 cut(s)	84, 265, 310, 349, 493, 739, 840, 1064, 1741
BstMAI	GTCTC	3 cut(s)	1013, 1436, 1722
BstMBI	GATC	9 cut(s)	81, 262, 307, 346, 490, 736, 837, 1061, 1738
BstMWI	GCNNNNNNNGC	3 cut(s)	123, 989, 1223
BstNI	CCWGG	1 cut(s)	653
BstNSI	RCATGY	3 cut(s)	190, 441, 1316
BstPI	GGTNACC	1 cut(s)	1824
BstSCI	CCNGG	4 cut(s)	121, 300, 651, 817
BstSFI	CTRYAG	1 cut(s)	1626
BstSLI	GKGCMC	1 cut(s)	1529
BstUI	CGCG	1 cut(s)	716
BstV1I	GCAGC	3 cut(s)	113, 612, 979
BstV2I	GAAGAC	1 cut(s)	750
BstX2I	RGATCY	3 cut(s)	81, 490, 1061
BstYI	RGATCY	3 cut(s)	81, 490, 1061
BsuI	GTATCC	1 cut(s)	1615
BsuRI	GGCC	8 cut(s)	38, 102, 243, 651, 687, 822, 1179, 1259
BtsCI	GGATG	9 cut(s)	66, 283, 415, 811, 1012, 1237, 1480, 1570, 1703
BtsI	GCAGTG	2 cut(s)	34, 861
BtsIMutI	CAGTG	5 cut(s)	34, 258, 546, 861, 1795
Cac8I	GCNNGC	5 cut(s)	100, 119, 439, 623, 1603
CaiI	CAGNNNCTG	3 cut(s)	224, 569, 1772
CciI	TCATGA	1 cut(s)	175
CfoI	GCGC	3 cut(s)	57, 92, 1516
Cfr13I	GGNCC	3 cut(s)	352, 530, 1178
CseI	GACGC	1 cut(s)	1033
Csp6I	GTAC	3 cut(s)	1632, 1659, 1676
CspCI	CAANNNNNGTGG	2 cut(s)	1119, 1154
CviAII	CATG	7 cut(s)	176, 187, 245, 268, 438, 1112, 1313
CviQI	GTAC	3 cut(s)	1632, 1659, 1676
DdeI	CTNAG	7 cut(s)	252, 487, 864, 1011, 1387, 1708, 1787
DinI	GGCGCC	2 cut(s)	91, 1515
DpnI	GATC	9 cut(s)	83, 264, 309, 348, 492, 738, 839, 1063, 1740
DpnII	GATC	9 cut(s)	81, 262, 307, 346, 490, 736, 837, 1061, 1738
EaeI	YGGCCR	4 cut(s)	36, 241, 649, 820
Eam1104I	CTCTTC	4 cut(s)	174, 420, 920, 1838
EarI	CTCTTC	4 cut(s)	174, 420, 920, 1838
EciI	GGCGGA	1 cut(s)	548
Ecl136II	GAGCTC	2 cut(s)	1669, 1786
Eco130I	CCWWGG	3 cut(s)	682, 1272, 1572
Eco147I	AGGCCT	1 cut(s)	687
Eco24I	GRGCYC	2 cut(s)	1671, 1788
Eco32I	GATATC	1 cut(s)	672
Eco47I	GGWCC	2 cut(s)	352, 530
Eco53kI	GAGCTC	2 cut(s)	1669, 1786
Eco57I	CTGAAG	3 cut(s)	444, 762, 1849
Eco72I	CACGTG	1 cut(s)	757
Eco88I	CYCGRG	1 cut(s)	799
Eco91I	GGTNACC	1 cut(s)	1824
EcoICRI	GAGCTC	2 cut(s)	1669, 1786
EcoNI	CCTNNNNNAGG	1 cut(s)	681
EcoO65I	GGTNACC	1 cut(s)	1824
EcoRII	CCWGG	1 cut(s)	651
EcoRV	GATATC	1 cut(s)	672
EcoT14I	CCWWGG	3 cut(s)	682, 1272, 1572
EcoT22I	ATGCAT	1 cut(s)	443
EcoT38I	GRGCYC	2 cut(s)	1671, 1788
EgeI	GGCGCC	2 cut(s)	91, 1515
EheI	GGCGCC	2 cut(s)	91, 1515
ErhI	CCWWGG	3 cut(s)	682, 1272, 1572
FaeI	CATG	7 cut(s)	179, 190, 248, 271, 441, 1115, 1316
FaqI	GGGAC	1 cut(s)	304
FatI	CATG	7 cut(s)	175, 186, 244, 267, 437, 1111, 1312
FauI	CCCGC	1 cut(s)	32
FauNDI	CATATG	1 cut(s)	1069
FbaI	TGATCA	1 cut(s)	307
Fnu4HI	GCNGC	3 cut(s)	127, 626, 993
FokI	GGATG	9 cut(s)	53, 290, 422, 818, 1019, 1244, 1487, 1577, 1690
FriOI	GRGCYC	2 cut(s)	1671, 1788
Fsp4HI	GCNGC	3 cut(s)	127, 626, 993
FspBI	CTAG	2 cut(s)	1830, 1881
GlaI	GCGC	3 cut(s)	56, 91, 1515
GluI	GCNGC	3 cut(s)	127, 626, 993
GsaI	CCCAGC	2 cut(s)	434, 1006
HaeII	RGCGCY	3 cut(s)	58, 93, 1517
HaeIII	GGCC	8 cut(s)	38, 102, 243, 651, 687, 822, 1179, 1259
HapII	CCGG	4 cut(s)	123, 301, 819, 1160
HgaI	GACGC	1 cut(s)	1033
HhaI	GCGC	3 cut(s)	57, 92, 1516
Hin1I	GRCGYC	2 cut(s)	90, 1514
Hin1II	CATG	7 cut(s)	179, 190, 248, 271, 441, 1115, 1316
Hin6I	GCGC	3 cut(s)	55, 90, 1514
HinP1I	GCGC	3 cut(s)	55, 90, 1514
HincII	GTYRAC	2 cut(s)	775, 1835
HindII	GTYRAC	2 cut(s)	775, 1835
HindIII	AAGCTT	2 cut(s)	338, 1215
HinfI	GANTC	4 cut(s)	696, 1268, 1697, 1848
HpaII	CCGG	4 cut(s)	123, 301, 819, 1160
HphI	GGTGA	4 cut(s)	860, 1294, 1348, 1818
Hpy166II	GTNNAC	7 cut(s)	258, 708, 775, 1285, 1527, 1676, 1835
Hpy188I	TCNGA	4 cut(s)	490, 1126, 1548, 1868
Hpy8I	GTNNAC	7 cut(s)	258, 708, 775, 1285, 1527, 1676, 1835
Hpy99I	CGWCG	1 cut(s)	1049
HpyAV	CCTTC	8 cut(s)	81, 226, 1072, 1249, 1410, 1456, 1756, 1774
HpyCH4III	ACNGT	6 cut(s)	772, 947, 1531, 1557, 1693, 1839
HpyCH4IV	ACGT	3 cut(s)	756, 1648, 1657
HpyF10VI	GCNNNNNNNGC	3 cut(s)	123, 989, 1223
HpyF3I	CTNAG	7 cut(s)	252, 487, 864, 1011, 1387, 1708, 1787
HpySE526I	ACGT	3 cut(s)	756, 1648, 1657
Hsp92I	GRCGYC	2 cut(s)	90, 1514
Hsp92II	CATG	7 cut(s)	179, 190, 248, 271, 441, 1115, 1316
HspAI	GCGC	3 cut(s)	55, 90, 1514
KasI	GGCGCC	2 cut(s)	89, 1513
KpnI	GGTACC	1 cut(s)	1635
Ksp22I	TGATCA	1 cut(s)	307
Kzo9I	GATC	9 cut(s)	81, 262, 307, 346, 490, 736, 837, 1061, 1738
LguI	GCTCTTC	1 cut(s)	420
LmnI	GCTCC	3 cut(s)	26, 382, 1783
Lsp1109I	GCAGC	3 cut(s)	113, 612, 979
LweI	GCATC	4 cut(s)	169, 245, 450, 997
MaeI	CTAG	2 cut(s)	1830, 1881
MaeII	ACGT	3 cut(s)	756, 1648, 1657
MaeIII	GTNAC	3 cut(s)	1336, 1711, 1824
MalI	GATC	9 cut(s)	83, 264, 309, 348, 492, 738, 839, 1063, 1740
MboI	GATC	9 cut(s)	81, 262, 307, 346, 490, 736, 837, 1061, 1738
MfeI	CAATTG	2 cut(s)	525, 1481
MflI	RGATCY	3 cut(s)	81, 490, 1061
MhlI	GDGCHC	5 cut(s)	548, 729, 1529, 1671, 1788
MlsI	TGGCCA	3 cut(s)	38, 243, 651
MluNI	TGGCCA	3 cut(s)	38, 243, 651
Mly113I	GGCGCC	2 cut(s)	90, 1514
MlyI	GAGTC	2 cut(s)	705, 1262
Mox20I	TGGCCA	3 cut(s)	38, 243, 651
Mph1103I	ATGCAT	1 cut(s)	443
MroXI	GAANNNNTTC	1 cut(s)	1847
MscI	TGGCCA	3 cut(s)	38, 243, 651
MseI	TTAA	6 cut(s)	273, 459, 1323, 1353, 1500, 1521
MslI	CAYNNNNRTG	1 cut(s)	584
Msp20I	TGGCCA	3 cut(s)	38, 243, 651
MspA1I	CMGCKG	1 cut(s)	422
MspI	CCGG	4 cut(s)	123, 301, 819, 1160
MspR9I	CCNGG	4 cut(s)	123, 302, 653, 819
MunI	CAATTG	2 cut(s)	525, 1481
Mva1269I	GAATGC	2 cut(s)	859, 1441
MvaI	CCWGG	1 cut(s)	653
MvnI	CGCG	1 cut(s)	716
MwoI	GCNNNNNNNGC	3 cut(s)	123, 989, 1223
NarI	GGCGCC	2 cut(s)	90, 1514
NciI	CCSGG	3 cut(s)	123, 302, 819
NdeI	CATATG	1 cut(s)	1069
NdeII	GATC	9 cut(s)	81, 262, 307, 346, 490, 736, 837, 1061, 1738
NlaIII	CATG	7 cut(s)	179, 190, 248, 271, 441, 1115, 1316
NlaIV	GGNNCC	4 cut(s)	91, 1515, 1550, 1633
NmeAIII	GCCGAG	1 cut(s)	1285
NmuCI	GTSAC	3 cut(s)	1336, 1711, 1824
NsiI	ATGCAT	1 cut(s)	443
NspI	RCATGY	3 cut(s)	190, 441, 1316
NspV	TTCGAA	1 cut(s)	661
PaeI	GCATGC	1 cut(s)	441
PagI	TCATGA	1 cut(s)	175
PceI	AGGCCT	1 cut(s)	687
PciI	ACATGT	2 cut(s)	186, 1312
PciSI	GCTCTTC	1 cut(s)	420
PcsI	WCGNNNNNNNCGW	1 cut(s)	1654
PctI	GAATGC	2 cut(s)	859, 1441
PdmI	GAANNNNTTC	1 cut(s)	1847
PfeI	GAWTC	2 cut(s)	1697, 1848
PkrI	GCNGC	3 cut(s)	128, 627, 994
PleI	GAGTC	2 cut(s)	704, 1262
PluTI	GGCGCC	2 cut(s)	93, 1517
PmaCI	CACGTG	1 cut(s)	757
PmlI	CACGTG	1 cut(s)	757
PpsI	GAGTC	2 cut(s)	704, 1262
Ppu21I	YACGTR	2 cut(s)	757, 1658
PscI	ACATGT	2 cut(s)	186, 1312
Psp124BI	GAGCTC	2 cut(s)	1671, 1788
Psp1406I	AACGTT	1 cut(s)	1648
Psp6I	CCWGG	1 cut(s)	651
PspCI	CACGTG	1 cut(s)	757
PspEI	GGTNACC	1 cut(s)	1824
PspFI	CCCAGC	2 cut(s)	430, 1002
PspGI	CCWGG	1 cut(s)	651
PspN4I	GGNNCC	4 cut(s)	91, 1515, 1550, 1633
PspPI	GGNCC	3 cut(s)	352, 530, 1178
PstNI	CAGNNNCTG	3 cut(s)	224, 569, 1772
PsuI	RGATCY	3 cut(s)	81, 490, 1061
PvuII	CAGCTG	1 cut(s)	422
RsaI	GTAC	3 cut(s)	1633, 1660, 1677
RsaNI	GTAC	3 cut(s)	1632, 1659, 1676
RseI	CAYNNNNRTG	1 cut(s)	584
SacI	GAGCTC	2 cut(s)	1671, 1788
SapI	GCTCTTC	1 cut(s)	420
SaqAI	TTAA	6 cut(s)	273, 459, 1323, 1353, 1500, 1521
SatI	GCNGC	3 cut(s)	127, 626, 993
Sau3AI	GATC	9 cut(s)	81, 262, 307, 346, 490, 736, 837, 1061, 1738
Sau96I	GGNCC	3 cut(s)	352, 530, 1178
SchI	GAGTC	2 cut(s)	705, 1262
ScrFI	CCNGG	4 cut(s)	123, 302, 653, 819
SduI	GDGCHC	5 cut(s)	548, 729, 1529, 1671, 1788
SfaNI	GCATC	4 cut(s)	169, 245, 450, 997
SfcI	CTRYAG	1 cut(s)	1626
SfoI	GGCGCC	2 cut(s)	91, 1515
SfuI	TTCGAA	1 cut(s)	661
SinI	GGWCC	2 cut(s)	352, 530
SmiMI	CAYNNNNRTG	1 cut(s)	584
SmlI	CTYRAG	4 cut(s)	134, 317, 642, 1103
SmoI	CTYRAG	4 cut(s)	134, 317, 642, 1103
SphI	GCATGC	1 cut(s)	441
SseBI	AGGCCT	1 cut(s)	687
SsiI	CCGC	3 cut(s)	25, 559, 716
SspDI	GGCGCC	2 cut(s)	89, 1513
SspMI	CTAG	2 cut(s)	1830, 1881
SstI	GAGCTC	2 cut(s)	1671, 1788
StuI	AGGCCT	1 cut(s)	687
StyD4I	CCNGG	4 cut(s)	121, 300, 651, 817
StyI	CCWWGG	3 cut(s)	682, 1272, 1572
TaaI	ACNGT	6 cut(s)	772, 947, 1531, 1557, 1693, 1839
TaiI	ACGT	3 cut(s)	759, 1651, 1660
TaqI	TCGA	6 cut(s)	542, 661, 1188, 1395, 1637, 1720
TfiI	GAWTC	2 cut(s)	1697, 1848
Tru1I	TTAA	6 cut(s)	273, 459, 1323, 1353, 1500, 1521
Tru9I	TTAA	6 cut(s)	273, 459, 1323, 1353, 1500, 1521
TscAI	CASTG	5 cut(s)	34, 265, 553, 861, 1795
TseFI	GTSAC	3 cut(s)	1336, 1711, 1824
TseI	GCWGC	3 cut(s)	126, 625, 992
Tsp45I	GTSAC	3 cut(s)	1336, 1711, 1824
TspDTI	ATGAA	9 cut(s)	164, 192, 822, 886, 1128, 1248, 1268, 1392, 1491
TspGWI	ACGGA	3 cut(s)	522, 689, 1736
TspRI	CASTG	5 cut(s)	34, 265, 553, 861, 1795
VneI	GTGCAC	1 cut(s)	1525
VpaK11BI	GGWCC	2 cut(s)	352, 530
XagI	CCTNNNNNAGG	1 cut(s)	681
XapI	RAATTY	4 cut(s)	589, 761, 1242, 1376
XbaI	TCTAGA	1 cut(s)	1880
XceI	RCATGY	3 cut(s)	190, 441, 1316
XmnI	GAANNNNTTC	1 cut(s)	1847
XspI	CTAG	2 cut(s)	1830, 1881
Zsp2I	ATGCAT	1 cut(s)	443

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.