Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0001310

Physical Location & Seq

Genomic Coordinates Reverse (-)

52230000 .. 52233983

Gene Structure 3984 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00370530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1944 bp

ATGGCCGCTTCTTCCTCCTCCGAGGATTCGCTCCGCAAAGCCCTCTCCGAGAAGCTCTCGGAGGTCGAGAGCCAGGGCAACCAGGTCCGTTCTCTGAAGAAATCCGGCGCCGCCAAGACGGAACTTGACGCCGCCATTGAGGCCTTGAACGCGCTCAAGCTTGAGAAGGCGTCGATCGAGAAAGACCTCCAGGCTACCATCAGCGGCGACGACGGCGGCTCCGTTAACAAGGAGGCGTTACGTCAGGCTGTGGTGAATACTCTGGAGCGCCGTTTGTTTTATATTCCCGCTTTCAAGATTTACGGCGGCGTTGCTGGCCTCTACGACTACGGGCCTCCTGGTTGCGCCGTCAAGTCCAATGTCCTAGGGTTTTGGCGTCAGCATTTTGTGCTTGAAGAGAACATGTTGGAAGTTGACTGCCCTTGTGTCACACCTGAGGTGGTTTTGAAAGCATCTGGTCATGTGGAGAAATTCACCGATCTTATGGTTAAGGATGAGGCGTCTGGGATGTGCTACAGAGCTGATCACTTGCTCAAGGATTACTGTAAAGATAAGCTTGAAAAGGACCTCAGAATTACTTCAGAGATGGCAATGGAACTGAAAGAAGTACTTTCCAAATTAGATGTCCTCTCCGCTGAAGAGCTGGGTGCATGTATAAAGAAATATGGTATTACAGCCCCTGATACAAAGAACACTCTCTCGGATCCTTATCCATTCAACCTGATGTTTCAAACATCAATTGGTCCTTCTGGTGTGAGCACCGGGTATATGCGTCCAGAAACTGCCCAAGGCATATTTGTGAATTTTAGGGACTTGTACTATTACAATGGGAACAAACTCCCTTTTGCTGCAGCCCAAATTGGCCAGGCTTTTAGAAATGAGATATCACCTCGCCAAGGTCTTTTGAGAGTCCGTGAGTTTACACTTGCAGAGATTGAGCACTTTGTTGACCCTGAAGACAAATCTCACCCAAAATTCTCTGATGTTGCACATTTGGAGTTTCTGATGTTCCCAAGGGATGAACAGAAGCCTGGCCAGTCTGCAAGGATAATCTCACTAGGTGAAGCTGTATCTTCTAAGGATTCTAAAATTGTCGACAACGAAACCCTTGGCTACTTTATTGGGAGGGTATACCTCTTCCTCACTCATCTGGGTATAGACAAGAACCGTTTAAGGTTCCGGCAGCACCTTGCAAATGAAATGGCTCACTATGCCCAGGACTGTTGGGATGCTGAGATTGAGACTTCATATGGCTGGATTGAGTGCGTGGGTATTGCAGATAGATCTGCGTATGACTTGAAGGCTCATTCGATGGTAGGATTTATAATCAAGGAAGCAATTGCTATTGTTGTTCTATTTAATACGTCTGGTTCAGTGGAGAAAAGTAGAGTTGATCTTGAGGCACAGGAAAAATATTCCGAACCAAGAGAAGTTGAGAAATTGGTTATATCTCCTGTAAAGAAAGCGATTGGTCTTGCTTTCAAGGGGGACCAAAAGAAAGTACTTGAAGCTTTGGAGGCCATGAAAGAAGAAGAAGCTTTGAAAATGAAGACTGATTTAGAGTCAAAAGGGGAGGTAGAGTTTTATGTCTGCACTCTAGAGAAGAAAGTATCTATTAATTCAAGTATGAAGCAAGAGTATGAGGAAGTTTCTAAAGTAATTTCCAAATCACTGACTGCATCTGGGATATCACATAAAATTGACATTACAGGTACCTCAATCGGGAAACGATACGCACGGACTGATGAACTGGGTGTACCGTTTGCGATCACAGTTGATTCCGTATCTTCTGTGACAATCCGGGAACGAGACAGCAAGGATCAGATCCGGGTTGATGTGAGTGAGGCAGCATCAGTTGTCAAGGATATAGCTGAGGGAGTGAGGACATGGGCGGATGTGTGGTCAGCTTTCCCACACCATTCTTCTGGCTCAGCAGACGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0003674 GO:0003824 GO:0004812 GO:0004820 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006426 GO:0006518 GO:0006520 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009117 GO:0009165 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0012505 GO:0015959 GO:0015960 GO:0015965 GO:0015966 GO:0016070 GO:0016874 GO:0016875 GO:0018130 GO:0019438 GO:0019538 GO:0019637 GO:0019752 GO:0031410 GO:0031982 GO:0031984 GO:0032543 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046686 GO:0046983 GO:0050896 GO:0055086 GO:0070127 GO:0070150 GO:0071704 GO:0090304 GO:0090407 GO:0097708 GO:0098791 GO:0140053 GO:0140098 GO:0140101 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon WHEP-TRS tRNA-synt_2b

Protein Analysis

647

Amino Acids

71.86

Weight (kDa)

5.41

Isoelectric Point (pI)

34.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHEP-TRS	PF00458	20 - 63	2.5e-09	WHEP-TRS domain
tRNA-synt_2b	PF00587	256 - 332	2.5e-09	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	531 - 622	2.4e-16	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29880
fragaria_vesca	FvH4_4g22441 FvH4_4g22450 FvH4_4g22450 FvH4_4g22610 FvH4_4g22610
malus_domestica	MD13G1131000.v1.1 MD13G1145800.v1.1 MD16G1141200.v1.1
prunus_persica	Prupe.1G214600_v2.0.a1
pyrus_communis	pycom13g11430 pycom13g12530 pycom16g11160 pycom16g11170
rosa_chinensis	RchiOBHm_Chr1g0361881 RchiOBHm_Chr4g0428711 RchiOBHm_Chr4g0428851
rosa_laevigata	RLG00000007120 RLG00000007133 RLG00000027689 RLG00000027690 RLG00000027694
rosa_multiflora	Rmu_co8208336.1_g000001 Rmu_co8210234.1_g000001 Rmu_sc0003316.1_g000006 Rmu_sc0008612.1_g000003 Rmu_sc0018216.1_g000001 Rmu_ssc0000124.1_g000013
rosa_roxburghii	Rroxscaffold_4G00294630 Rroxscaffold_5G00370530
rosa_rugosa	Rorug01G0292100 Rorug04G0227400 Rorug04G0227500 Rorug04G0229700
rosa_samantha	Rh1AG302800 Rh1BG265900 Rh1BG266000 Rh1CG284000 Rh1DG295900 Rh1DG296000 Rh4AG284700 Rh4AG284900 Rh4AG286400 Rh4BG290700 Rh4BG290900 Rh4BG292200 Rh4CG308200 Rh4DG288200 Rh4DG289400
rosa_wichuraiana	Rw0G020620 Rw1G026830 Rw4G024720 Rw4G024830 Rw7G040280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1325
Acc65I	GGTACC	1 cut(s)	1712
AccB1I	GGYRCC	2 cut(s)	107, 1712
AccI	GTMKAC	2 cut(s)	1095, 1131
AccII	CGCG	1 cut(s)	152
AclWI	GGATC	4 cut(s)	698, 711, 1819, 1827
AcoI	YGGCCR	3 cut(s)	3, 862, 1033
AcsI	RAATTY	3 cut(s)	470, 802, 974
AcuI	CTGAAG	4 cut(s)	116, 564, 657, 975
AcyI	GRCGYC	5 cut(s)	108, 129, 170, 376, 500
AdeI	CACNNNGTG	1 cut(s)	1061
AfaI	GTAC	5 cut(s)	609, 818, 1503, 1714, 1758
AfiI	CCNNNNNNNGG	2 cut(s)	896, 1722
AflIII	ACRYGT	1 cut(s)	402
AjnI	CCWGG	7 cut(s)	72, 81, 189, 337, 864, 1030, 1215
Alw21I	GWGCWC	2 cut(s)	761, 942
Alw26I	GTCTC	2 cut(s)	1235, 1803
AlwI	GGATC	4 cut(s)	698, 711, 1819, 1827
AlwNI	CAGNNNCTG	2 cut(s)	680, 782
AoxI	GGCC	7 cut(s)	3, 141, 316, 332, 862, 1033, 1518
ApeKI	GCWGC	4 cut(s)	848, 851, 1183, 1847
ApoI	RAATTY	3 cut(s)	470, 802, 974
ArsI	GACNNNNNNTTYG	2 cut(s)	609, 641
AseI	ATTAAT	1 cut(s)	1617
Asp700I	GAANNNNTTC	1 cut(s)	577
Asp718I	GGTACC	1 cut(s)	1712
AspA2I	CCTAGG	1 cut(s)	364
AspLEI	GCGC	4 cut(s)	110, 154, 270, 347
AspS9I	GGNCC	5 cut(s)	85, 332, 565, 743, 1489
AsuC2I	CCSGG	3 cut(s)	763, 1802, 1829
AsuHPI	GGTGA	5 cut(s)	265, 466, 879, 959, 1073
AvaII	GGWCC	4 cut(s)	85, 565, 743, 1489
AvrII	CCTAGG	1 cut(s)	364
AxyI	CCTNAGG	1 cut(s)	435
BalI	TGGCCA	2 cut(s)	864, 1035
BamHI	GGATCC	1 cut(s)	703
BanI	GGYRCC	2 cut(s)	107, 1712
BbsI	GAAGAC	2 cut(s)	963, 1556
Bbv12I	GWGCWC	2 cut(s)	761, 942
BbvCI	CCTCAGC	1 cut(s)	1872
BbvI	GCAGC	4 cut(s)	835, 863, 1195, 1859
BccI	CCATC	3 cut(s)	206, 580, 1306
BceAI	ACGGC	4 cut(s)	229, 255, 319, 332
BciT130I	CCWGG	7 cut(s)	74, 83, 191, 339, 866, 1032, 1217
BclI	TGATCA	1 cut(s)	523
BcnI	CCSGG	3 cut(s)	763, 1802, 1829
BcoDI	GTCTC	2 cut(s)	1235, 1803
BfaI	CTAG	3 cut(s)	365, 1058, 1598
BfmI	CTRYAG	2 cut(s)	514, 849
BfoI	RGCGCY	2 cut(s)	111, 271
BglI	GCCNNNNNGGC	1 cut(s)	140
BglII	AGATCT	1 cut(s)	1283
BlnI	CCTAGG	1 cut(s)	364
BlpI	GCTNAGC	1 cut(s)	1930
BmcAI	AGTACT	2 cut(s)	609, 1503
Bme18I	GGWCC	4 cut(s)	85, 565, 743, 1489
BmgT120I	GGNCC	5 cut(s)	85, 332, 565, 743, 1489
BmiI	GGNNCC	6 cut(s)	109, 220, 705, 1178, 1490, 1714
BmrI	ACTGGG	1 cut(s)	1760
BmsI	GCATC	4 cut(s)	461, 1219, 1688, 1859
BmuI	ACTGGG	1 cut(s)	1760
BpiI	GAAGAC	2 cut(s)	963, 1556
BplI	GAGNNNNNCTC	2 cut(s)	41, 73
BpmI	CTGGAG	2 cut(s)	173, 284
Bpu10I	CCTNAGC	1 cut(s)	1872
Bpu1102I	GCTNAGC	1 cut(s)	1930
BpuEI	CTTGAG	4 cut(s)	140, 182, 518, 1418
BpuMI	CCSGG	3 cut(s)	763, 1802, 1829
BsaBI	GATNNNNATC	1 cut(s)	708
BsaHI	GRCGYC	5 cut(s)	108, 129, 170, 376, 500
BsaJI	CCNNGG	8 cut(s)	21, 73, 364, 787, 895, 1013, 1108, 1215
BsaXI	ACNNNNNCTCC	4 cut(s)	203, 233, 1565, 1595
Bsc4I	CCNNNNNNNGG	2 cut(s)	896, 1722
Bse1I	ACTGG	2 cut(s)	1036, 1755
Bse21I	CCTNAGG	1 cut(s)	435
Bse3DI	GCAATG	1 cut(s)	597
Bse8I	GATNNNNATC	1 cut(s)	708
BseBI	CCWGG	7 cut(s)	74, 83, 191, 339, 866, 1032, 1217
BseDI	CCNNGG	8 cut(s)	21, 73, 364, 787, 895, 1013, 1108, 1215
BseGI	GGATG	5 cut(s)	499, 513, 1024, 1234, 1900
BseJI	GATNNNNATC	1 cut(s)	708
BseLI	CCNNNNNNNGG	2 cut(s)	896, 1722
BseMI	GCAATG	1 cut(s)	597
BseMII	CTCAG	5 cut(s)	426, 583, 1224, 1863, 1944
BseNI	ACTGG	2 cut(s)	1036, 1755
BseRI	GAGGAG	1 cut(s)	7
BseXI	GCAGC	4 cut(s)	835, 863, 1195, 1859
BseYI	CCCAGC	1 cut(s)	643
BsgI	GTGCAG	1 cut(s)	1576
Bsh1236I	CGCG	1 cut(s)	152
Bsh1285I	CGRYCG	1 cut(s)	177
BshFI	GGCC	7 cut(s)	5, 143, 318, 334, 864, 1035, 1520
BshNI	GGYRCC	2 cut(s)	107, 1712
BsiEI	CGRYCG	1 cut(s)	177
BsiHKAI	GWGCWC	2 cut(s)	761, 942
BsiSI	CCGG	5 cut(s)	105, 762, 1180, 1801, 1828
BslFI	GGGAC	2 cut(s)	824, 1502
BslI	CCNNNNNNNGG	2 cut(s)	896, 1722
BsmAI	GTCTC	2 cut(s)	1235, 1803
BsmFI	GGGAC	2 cut(s)	824, 1502
BsnI	GGCC	7 cut(s)	5, 143, 318, 334, 864, 1035, 1520
Bsp1286I	GDGCHC	2 cut(s)	761, 942
Bsp143I	GATC	9 cut(s)	174, 478, 523, 703, 1283, 1393, 1767, 1819, 1824
Bsp1720I	GCTNAGC	1 cut(s)	1930
BspANI	GGCC	7 cut(s)	5, 143, 318, 334, 864, 1035, 1520
BspCNI	CTCAG	5 cut(s)	427, 582, 1225, 1864, 1943
BspFNI	CGCG	1 cut(s)	152
BspLI	GGNNCC	6 cut(s)	109, 220, 705, 1178, 1490, 1714
BspMAI	CTGCAG	1 cut(s)	853
BspPI	GGATC	4 cut(s)	698, 711, 1819, 1827
BspQI	GCTCTTC	1 cut(s)	633
BspT107I	GGYRCC	2 cut(s)	107, 1712
BsrDI	GCAATG	1 cut(s)	597
BsrI	ACTGG	2 cut(s)	1036, 1755
BssECI	CCNNGG	8 cut(s)	21, 73, 364, 787, 895, 1013, 1108, 1215
BssMI	GATC	9 cut(s)	174, 478, 523, 703, 1283, 1393, 1767, 1819, 1824
BssNAI	GTATAC	1 cut(s)	1132
BssNI	GRCGYC	5 cut(s)	108, 129, 170, 376, 500
BssT1I	CCWWGG	5 cut(s)	364, 787, 895, 1013, 1108
Bst1107I	GTATAC	1 cut(s)	1132
Bst2UI	CCWGG	7 cut(s)	74, 83, 191, 339, 866, 1032, 1217
Bst4CI	ACNGT	5 cut(s)	545, 1169, 1223, 1761, 1774
Bst6I	CTCTTC	3 cut(s)	390, 633, 1142
BstACI	GRCGYC	5 cut(s)	108, 129, 170, 376, 500
BstAPI	GCANNNNNTGC	1 cut(s)	388
BstC8I	GCNNGC	1 cut(s)	316
BstDEI	CTNAG	6 cut(s)	435, 569, 1077, 1233, 1872, 1930
BstENI	CCTNNNNNAGG	1 cut(s)	894
BstF5I	GGATG	5 cut(s)	499, 513, 1024, 1234, 1900
BstFNI	CGCG	1 cut(s)	152
BstH2I	RGCGCY	2 cut(s)	111, 271
BstHHI	GCGC	4 cut(s)	110, 154, 270, 347
BstKTI	GATC	9 cut(s)	177, 481, 526, 706, 1286, 1396, 1770, 1822, 1827
BstMAI	GTCTC	2 cut(s)	1235, 1803
BstMBI	GATC	9 cut(s)	174, 478, 523, 703, 1283, 1393, 1767, 1819, 1824
BstMCI	CGRYCG	1 cut(s)	177
BstMWI	GCNNNNNNNGC	7 cut(s)	140, 149, 213, 315, 388, 1211, 1517
BstNI	CCWGG	7 cut(s)	74, 83, 191, 339, 866, 1032, 1217
BstNSI	RCATGY	2 cut(s)	406, 654
BstSFI	CTRYAG	2 cut(s)	514, 849
BstUI	CGCG	1 cut(s)	152
BstV1I	GCAGC	4 cut(s)	835, 863, 1195, 1859
BstV2I	GAAGAC	2 cut(s)	963, 1556
BstX2I	RGATCY	3 cut(s)	703, 1283, 1824
BstXI	CCANNNNNNTGG	1 cut(s)	1925
BstYI	RGATCY	3 cut(s)	703, 1283, 1824
BstZ17I	GTATAC	1 cut(s)	1132
Bsu36I	CCTNAGG	1 cut(s)	435
BsuRI	GGCC	7 cut(s)	5, 143, 318, 334, 864, 1035, 1520
BtsCI	GGATG	5 cut(s)	499, 513, 1024, 1234, 1900
BtsIMutI	CAGTG	2 cut(s)	1380, 1670
Cac8I	GCNNGC	1 cut(s)	316
CaiI	CAGNNNCTG	2 cut(s)	680, 782
CfoI	GCGC	4 cut(s)	110, 154, 270, 347
Cfr13I	GGNCC	5 cut(s)	85, 332, 565, 743, 1489
CseI	GACGC	5 cut(s)	137, 159, 365, 489, 761
CsiI	ACCWGGT	1 cut(s)	81
Csp6I	GTAC	5 cut(s)	608, 817, 1502, 1713, 1757
CviAII	CATG	5 cut(s)	403, 461, 651, 1522, 1887
CviQI	GTAC	5 cut(s)	608, 817, 1502, 1713, 1757
DdeI	CTNAG	6 cut(s)	435, 569, 1077, 1233, 1872, 1930
DinI	GGCGCC	1 cut(s)	109
DpnI	GATC	9 cut(s)	176, 480, 525, 705, 1285, 1395, 1769, 1821, 1826
DpnII	GATC	9 cut(s)	174, 478, 523, 703, 1283, 1393, 1767, 1819, 1824
DraIII	CACNNNGTG	1 cut(s)	1061
EaeI	YGGCCR	3 cut(s)	3, 862, 1033
Eam1104I	CTCTTC	3 cut(s)	390, 633, 1142
EarI	CTCTTC	3 cut(s)	390, 633, 1142
EciI	GGCGGA	1 cut(s)	1907
Eco130I	CCWWGG	5 cut(s)	364, 787, 895, 1013, 1108
Eco147I	AGGCCT	1 cut(s)	143
Eco32I	GATATC	2 cut(s)	885, 1689
Eco47I	GGWCC	4 cut(s)	85, 565, 743, 1489
Eco57I	CTGAAG	4 cut(s)	116, 564, 657, 975
Eco81I	CCTNAGG	1 cut(s)	435
EcoNI	CCTNNNNNAGG	1 cut(s)	894
EcoO109I	RGGNCCY	1 cut(s)	565
EcoRII	CCWGG	7 cut(s)	72, 81, 189, 337, 864, 1030, 1215
EcoRV	GATATC	2 cut(s)	885, 1689
EcoT14I	CCWWGG	5 cut(s)	364, 787, 895, 1013, 1108
EgeI	GGCGCC	1 cut(s)	109
EheI	GGCGCC	1 cut(s)	109
ErhI	CCWWGG	5 cut(s)	364, 787, 895, 1013, 1108
FaeI	CATG	5 cut(s)	406, 464, 654, 1525, 1890
FalI	AAGNNNNNCTT	4 cut(s)	540, 572, 594, 626
FaqI	GGGAC	2 cut(s)	824, 1502
FatI	CATG	5 cut(s)	402, 460, 650, 1521, 1886
FauI	CCCGC	1 cut(s)	295
FauNDI	CATATG	1 cut(s)	1249
FbaI	TGATCA	1 cut(s)	523
FblI	GTMKAC	2 cut(s)	1095, 1131
FokI	GGATG	5 cut(s)	506, 520, 1031, 1241, 1907
FspBI	CTAG	3 cut(s)	365, 1058, 1598
GlaI	GCGC	4 cut(s)	109, 153, 269, 346
GsaI	CCCAGC	1 cut(s)	647
GsuI	CTGGAG	2 cut(s)	173, 284
HaeII	RGCGCY	2 cut(s)	111, 271
HaeIII	GGCC	7 cut(s)	5, 143, 318, 334, 864, 1035, 1520
HapII	CCGG	5 cut(s)	105, 762, 1180, 1801, 1828
HgaI	GACGC	5 cut(s)	137, 159, 365, 489, 761
HhaI	GCGC	4 cut(s)	110, 154, 270, 347
Hin1I	GRCGYC	5 cut(s)	108, 129, 170, 376, 500
Hin1II	CATG	5 cut(s)	406, 464, 654, 1525, 1890
Hin6I	GCGC	4 cut(s)	108, 152, 268, 345
HinP1I	GCGC	4 cut(s)	108, 152, 268, 345
HincII	GTYRAC	4 cut(s)	226, 415, 949, 1096
HindII	GTYRAC	4 cut(s)	226, 415, 949, 1096
HindIII	AAGCTT	4 cut(s)	158, 554, 1509, 1536
HinfI	GANTC	5 cut(s)	26, 909, 1082, 1562, 1778
HpaI	GTTAAC	1 cut(s)	226
HpaII	CCGG	5 cut(s)	105, 762, 1180, 1801, 1828
HphI	GGTGA	5 cut(s)	265, 466, 879, 959, 1073
Hpy166II	GTNNAC	7 cut(s)	226, 415, 921, 949, 1096, 1132, 1757
Hpy188III	TCNNGA	8 cut(s)	67, 178, 263, 295, 776, 1397, 1598, 1723
Hpy8I	GTNNAC	7 cut(s)	226, 415, 921, 949, 1096, 1132, 1757
Hpy99I	CGWCG	3 cut(s)	175, 212, 215
HpyAV	CCTTC	3 cut(s)	160, 756, 1294
HpyCH4III	ACNGT	5 cut(s)	545, 1169, 1223, 1761, 1774
HpyCH4IV	ACGT	2 cut(s)	241, 1364
HpyCH4V	TGCA	9 cut(s)	650, 851, 929, 989, 1043, 1193, 1277, 1593, 1679
HpyF10VI	GCNNNNNNNGC	7 cut(s)	140, 149, 213, 315, 388, 1211, 1517
HpyF3I	CTNAG	6 cut(s)	435, 569, 1077, 1233, 1872, 1930
HpySE526I	ACGT	2 cut(s)	241, 1364
Hsp92I	GRCGYC	5 cut(s)	108, 129, 170, 376, 500
Hsp92II	CATG	5 cut(s)	406, 464, 654, 1525, 1890
HspAI	GCGC	4 cut(s)	108, 152, 268, 345
KasI	GGCGCC	1 cut(s)	107
KpnI	GGTACC	1 cut(s)	1716
Ksp22I	TGATCA	1 cut(s)	523
KspAI	GTTAAC	1 cut(s)	226
Kzo9I	GATC	9 cut(s)	174, 478, 523, 703, 1283, 1393, 1767, 1819, 1824
LguI	GCTCTTC	1 cut(s)	633
LmnI	GCTCC	3 cut(s)	36, 224, 265
Lsp1109I	GCAGC	4 cut(s)	835, 863, 1195, 1859
LweI	GCATC	4 cut(s)	461, 1219, 1688, 1859
MabI	ACCWGGT	1 cut(s)	81
MaeI	CTAG	3 cut(s)	365, 1058, 1598
MaeII	ACGT	2 cut(s)	241, 1364
MaeIII	GTNAC	3 cut(s)	237, 427, 1792
MalI	GATC	9 cut(s)	176, 480, 525, 705, 1285, 1395, 1769, 1821, 1826
MboI	GATC	9 cut(s)	174, 478, 523, 703, 1283, 1393, 1767, 1819, 1824
MfeI	CAATTG	2 cut(s)	738, 1338
MflI	RGATCY	3 cut(s)	703, 1283, 1824
MhlI	GDGCHC	2 cut(s)	761, 942
MlsI	TGGCCA	2 cut(s)	864, 1035
MluNI	TGGCCA	2 cut(s)	864, 1035
Mly113I	GGCGCC	1 cut(s)	108
MlyI	GAGTC	2 cut(s)	918, 1571
MmeI	TCCRAC	1 cut(s)	387
Mox20I	TGGCCA	2 cut(s)	864, 1035
MroXI	GAANNNNTTC	1 cut(s)	577
MscI	TGGCCA	2 cut(s)	864, 1035
MseI	TTAA	5 cut(s)	225, 489, 1172, 1359, 1617
MslI	CAYNNNNRTG	1 cut(s)	797
Msp20I	TGGCCA	2 cut(s)	864, 1035
MspA1I	CMGCKG	2 cut(s)	204, 635
MspI	CCGG	5 cut(s)	105, 762, 1180, 1801, 1828
MunI	CAATTG	2 cut(s)	738, 1338
MvaI	CCWGG	7 cut(s)	74, 83, 191, 339, 866, 1032, 1217
MvnI	CGCG	1 cut(s)	152
MwoI	GCNNNNNNNGC	7 cut(s)	140, 149, 213, 315, 388, 1211, 1517
NarI	GGCGCC	1 cut(s)	108
NciI	CCSGG	3 cut(s)	763, 1802, 1829
NdeI	CATATG	1 cut(s)	1249
NdeII	GATC	9 cut(s)	174, 478, 523, 703, 1283, 1393, 1767, 1819, 1824
NlaIII	CATG	5 cut(s)	406, 464, 654, 1525, 1890
NlaIV	GGNNCC	6 cut(s)	109, 220, 705, 1178, 1490, 1714
NmuCI	GTSAC	2 cut(s)	427, 1792
NspI	RCATGY	2 cut(s)	406, 654
PceI	AGGCCT	1 cut(s)	143
PciI	ACATGT	1 cut(s)	402
PciSI	GCTCTTC	1 cut(s)	633
PcsI	WCGNNNNNNNCGW	1 cut(s)	219
PdmI	GAANNNNTTC	1 cut(s)	577
PfeI	GAWTC	3 cut(s)	26, 1082, 1778
PfoI	TCCNGGA	1 cut(s)	1800
Ple19I	CGATCG	1 cut(s)	177
PleI	GAGTC	2 cut(s)	917, 1570
PluTI	GGCGCC	1 cut(s)	111
PpsI	GAGTC	2 cut(s)	917, 1570
PpuMI	RGGWCCY	1 cut(s)	565
PscI	ACATGT	1 cut(s)	402
PshBI	ATTAAT	1 cut(s)	1617
PsiI	TTATAA	1 cut(s)	1325
Psp5II	RGGWCCY	1 cut(s)	565
Psp6I	CCWGG	7 cut(s)	72, 81, 189, 337, 864, 1030, 1215
PspFI	CCCAGC	1 cut(s)	643
PspGI	CCWGG	7 cut(s)	72, 81, 189, 337, 864, 1030, 1215
PspN4I	GGNNCC	6 cut(s)	109, 220, 705, 1178, 1490, 1714
PspPI	GGNCC	5 cut(s)	85, 332, 565, 743, 1489
PspPPI	RGGWCCY	1 cut(s)	565
PsrI	GAACNNNNNNTAC	2 cut(s)	1740, 1772
PstI	CTGCAG	1 cut(s)	853
PstNI	CAGNNNCTG	2 cut(s)	680, 782
PsuI	RGATCY	3 cut(s)	703, 1283, 1824
PvuI	CGATCG	1 cut(s)	177
RsaI	GTAC	5 cut(s)	609, 818, 1503, 1714, 1758
RsaNI	GTAC	5 cut(s)	608, 817, 1502, 1713, 1757
RseI	CAYNNNNRTG	1 cut(s)	797
SalI	GTCGAC	1 cut(s)	1094
SapI	GCTCTTC	1 cut(s)	633
SaqAI	TTAA	5 cut(s)	225, 489, 1172, 1359, 1617
Sau3AI	GATC	9 cut(s)	174, 478, 523, 703, 1283, 1393, 1767, 1819, 1824
Sau96I	GGNCC	5 cut(s)	85, 332, 565, 743, 1489
ScaI	AGTACT	2 cut(s)	609, 1503
SchI	GAGTC	2 cut(s)	918, 1571
SduI	GDGCHC	2 cut(s)	761, 942
SexAI	ACCWGGT	1 cut(s)	81
SfaNI	GCATC	4 cut(s)	461, 1219, 1688, 1859
SfcI	CTRYAG	2 cut(s)	514, 849
SfoI	GGCGCC	1 cut(s)	109
SinI	GGWCC	4 cut(s)	85, 565, 743, 1489
SmiMI	CAYNNNNRTG	1 cut(s)	797
SmlI	CTYRAG	4 cut(s)	155, 161, 533, 1397
SmoI	CTYRAG	4 cut(s)	155, 161, 533, 1397
SseBI	AGGCCT	1 cut(s)	143
SspDI	GGCGCC	1 cut(s)	107
SspI	AATATT	1 cut(s)	1415
SspMI	CTAG	3 cut(s)	365, 1058, 1598
StuI	AGGCCT	1 cut(s)	143
StyI	CCWWGG	5 cut(s)	364, 787, 895, 1013, 1108
TaaI	ACNGT	5 cut(s)	545, 1169, 1223, 1761, 1774
TaiI	ACGT	2 cut(s)	244, 1367
TaqI	TCGA	5 cut(s)	66, 173, 177, 1095, 1310
TatI	WGTACW	3 cut(s)	607, 816, 1501
TauI	GCSGC	6 cut(s)	8, 113, 134, 207, 219, 309
TfiI	GAWTC	3 cut(s)	26, 1082, 1778
Tru1I	TTAA	5 cut(s)	225, 489, 1172, 1359, 1617
Tru9I	TTAA	5 cut(s)	225, 489, 1172, 1359, 1617
TscAI	CASTG	2 cut(s)	1380, 1677
TseFI	GTSAC	2 cut(s)	427, 1792
TseI	GCWGC	4 cut(s)	848, 851, 1183, 1847
Tsp45I	GTSAC	2 cut(s)	427, 1792
TspDTI	ATGAA	7 cut(s)	1035, 1212, 1236, 1538, 1562, 1643, 1761
TspGWI	ACGGA	6 cut(s)	77, 134, 211, 902, 1753, 1771
TspRI	CASTG	2 cut(s)	1380, 1677
VpaK11BI	GGWCC	4 cut(s)	85, 565, 743, 1489
VspI	ATTAAT	1 cut(s)	1617
XagI	CCTNNNNNAGG	1 cut(s)	894
XapI	RAATTY	3 cut(s)	470, 802, 974
XbaI	TCTAGA	1 cut(s)	1597
XceI	RCATGY	2 cut(s)	406, 654
XmaJI	CCTAGG	1 cut(s)	364
XmiI	GTMKAC	2 cut(s)	1095, 1131
XmnI	GAANNNNTTC	1 cut(s)	577
XspI	CTAG	3 cut(s)	365, 1058, 1598
ZrmI	AGTACT	2 cut(s)	609, 1503

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.