Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001310

Physical Location & Seq

Genomic Coordinates Forward (+)

10884477 .. 10888998

Gene Structure 4522 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1141200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2070 bp

ATGGCCGCCCCGGAAGATTCGCTCCGTAAAGCTCTCGCCGACAAGCAGTCGGAGGTTGAAAGCCAGGGGAGCCAAGTGCGGGCTTTGAAGGCCCGCAAGGCTGACAAGTCGGAAATTGACGCCGCCATTGCAGCTCTGAATGCGCTCAAGCTGGAGAAAGCTTCGATCGAGAAGGACCTCCAGGCCACATTGTGCAGCGGCGAAGGGTCCGGTAACAAGGAGGCGTTCCGTCAGGCGGTGTCGAATACGCTTGAACGGCGTCTCTTCTACATCAAGTCTTTCAACATCTACGGTGGCGTTGCGGGACTGTATGACTACGGCCCGCCCGGTTGTGCCGTCAAGTCCAATGTGCTAGCTTTTTGGCGGCAGCATTTTGTTCTTGAGGAGAACATGCTGGAAGTTGATTGCCCCTGTGTCACACCCGAGGTGGTTCTGAAGGCATCTGGCCATGTCGAAAAATTCACTGATCTTATGGTGAAAGACGAGAAAACTGGGACTTGCTACCGAGCGGATCACTTGCTGAAGGATTTTTGTAATGATAAGCTTCAAAAGGATCTCAACATTACTGCAGAGAAGGCCAAGGAGCTTAAACATATACTTGCGGTGTTGGATGATCTCTCACCTGAAGATCTAGGTGCAAAGATTAAGGAGTTTGGTATTACTGCCCCTGACACGAAGAATCCTCTATCTGATCCTTATCCTTTCAACCTGATGTTTCAAACTTCAATTGGTCCGTCTGGCTTGATCCCTGGGTATTTGCGTCCAGAGACTGCCCAAGGCATATTTGTGAATTTCAAAGATCTGTATTATTACAATGGCAACAAGCTACCTTTTGCTGCAGCTCAGATAGGACAAGCTTTTAGAAATGAGATATCTCCTCGCCAAGGCCTTCTGAGAGTTCGGGAATTCACACTGGCAGAGATTGAGCATTTTGTAGATCCCCAAGACAAATCTCACCCAAAGTTCACAGAGGTTGCAGAGTTGGAGTTTTTGATGTTTCCTAGGGAATTACAAATGTCAGGGGAATCTGCAAAGACATTCCGTTTGGGTGAATCAGTTGCTAAGGGAATTGTCAACAATGAAACTTTGGGCTACTTCATTGGAAGAGTATATCTTTTCCTTACTCGTTTAGGTATTGACAAGAAACGGTTAAGGTTCCGACAGCACCTTGCAAATGAAATGGCTCACTATGCTGCTGATTGTTGGGATGCTGAGATTGAGAGTTCCTATGGCTGGATTGAGTGTGTTGGTATAGCTGATAGATCTGCATTTGATTTGCGTGCTCATACGGAGAAAAGTGGTGAAGCTCTTGTGGCTCATGAAAAATATCCAGAACCAAGAGAAGTAGAGGTATTGGTTATAACTCCAGTAAAAAAAGAGATTGGCCTTGCATTCAAGGGGAACCAAAAGAATGTAATTGAAGCGTTGGAGTCCATGAAAGAACAAGAAGCTATGGCATTGAAGGCTGATTTAGAGTCTAAAGGGGAGGTGGAGTTTTACGTCTGCACGCTGGGGCAGAATGTAACCATTAAGAAGAGTATGGTAAAAATTTCAAAGGAGAAAAAGAAAGAACACCAGAGAGTATTCACGCCTTCTGTAATTGAACCGTCTTTTGGTATTGGCCGAATTATATATTGCCTCTTTGAGCACACGTACTACACGAGGCCTAGTAAAGCTGCTGACCAACAGTTGAATGTTTTTGGTTTCCCCCCACTTGTGGCACCCATCAAGTGCACAGTTTTCCCTCTGGTTCAGAACCATCAGTATGAGGATGTTGCTAAAGAAATTTCCAAGTCATTGACTGCTGCTGGAATCTCACATAAAATTGACATTACAGGTACCTCGATTGGGAAAAGATATGCTCGAACAGATGAACTGGGTGTACCTTTTGCAATCACGGTTGATTCAACATCATCGGTGACACTGAGAGAGAGGGACAGCAAGGATCAAATCCGTGTTGATGTGAAAGAGGCAGCATCTGTGGTGCAGGGTGTAGCTGAGGGAGTGAGGAGCTGGGCTGATGTGTGGGAGACGTTCCCCCATCATTCGTCTGCATCTGCAGATGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0003674 GO:0003824 GO:0004812 GO:0004820 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006426 GO:0006518 GO:0006520 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009117 GO:0009165 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0012505 GO:0015959 GO:0015960 GO:0015965 GO:0015966 GO:0016070 GO:0016874 GO:0016875 GO:0018130 GO:0019438 GO:0019538 GO:0019637 GO:0019752 GO:0031410 GO:0031982 GO:0031984 GO:0032543 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046686 GO:0046983 GO:0050896 GO:0055086 GO:0070127 GO:0070150 GO:0071704 GO:0090304 GO:0090407 GO:0097708 GO:0098791 GO:0140053 GO:0140098 GO:0140101 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon WHEP-TRS tRNA-synt_2b

Protein Analysis

690

Amino Acids

76.89

Weight (kDa)

5.99

Isoelectric Point (pI)

38.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHEP-TRS	PF00458	17 - 60	1.9e-10	WHEP-TRS domain
tRNA-synt_2b	PF00587	252 - 550	6.8e-11	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	577 - 663	2.8e-19	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29880
fragaria_vesca	FvH4_4g22441 FvH4_4g22450 FvH4_4g22450 FvH4_4g22610 FvH4_4g22610
malus_domestica	MD13G1131000.v1.1 MD13G1145800.v1.1 MD16G1141200.v1.1
prunus_persica	Prupe.1G214600_v2.0.a1
pyrus_communis	pycom13g11430 pycom13g12530 pycom16g11160 pycom16g11170
rosa_chinensis	RchiOBHm_Chr1g0361881 RchiOBHm_Chr4g0428711 RchiOBHm_Chr4g0428851
rosa_laevigata	RLG00000007120 RLG00000007133 RLG00000027689 RLG00000027690 RLG00000027694
rosa_multiflora	Rmu_co8208336.1_g000001 Rmu_co8210234.1_g000001 Rmu_sc0003316.1_g000006 Rmu_sc0008612.1_g000003 Rmu_sc0018216.1_g000001 Rmu_ssc0000124.1_g000013
rosa_roxburghii	Rroxscaffold_4G00294630 Rroxscaffold_5G00370530
rosa_rugosa	Rorug01G0292100 Rorug04G0227400 Rorug04G0227500 Rorug04G0229700
rosa_samantha	Rh1AG302800 Rh1BG265900 Rh1BG266000 Rh1CG284000 Rh1DG295900 Rh1DG296000 Rh4AG284700 Rh4AG284900 Rh4AG286400 Rh4BG290700 Rh4BG290900 Rh4BG292200 Rh4CG308200 Rh4DG288200 Rh4DG289400
rosa_wichuraiana	Rw0G020620 Rw1G026830 Rw4G024720 Rw4G024830 Rw7G040280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1361
Acc65I	GGTACC	1 cut(s)	1838
AccB1I	GGYRCC	2 cut(s)	1720, 1838
AccBSI	CCGCTC	1 cut(s)	509
AclWI	GGATC	6 cut(s)	519, 561, 686, 739, 932, 1953
AcoI	YGGCCR	3 cut(s)	3, 445, 1621
AcsI	RAATTY	5 cut(s)	458, 790, 905, 1548, 1785
AcuI	CTGAAG	3 cut(s)	455, 542, 645
AcyI	GRCGYC	2 cut(s)	120, 259
AdeI	CACNNNGTG	1 cut(s)	192
AfaI	GTAC	3 cut(s)	1655, 1840, 1884
AfiI	CCNNNNNNNGG	7 cut(s)	79, 235, 884, 1233, 1613, 1717, 1848
AflIII	ACRYGT	1 cut(s)	1650
AhdI	GACNNNNNGTC	1 cut(s)	46
AjnI	CCWGG	3 cut(s)	63, 180, 748
AjuI	GAANNNNNNNTTGG	2 cut(s)	1596, 1628
Alw21I	GWGCWC	3 cut(s)	1285, 1650, 1736
Alw26I	GTCTC	3 cut(s)	266, 761, 2024
Alw44I	GTGCAC	1 cut(s)	1732
AlwI	GGATC	6 cut(s)	519, 561, 686, 739, 932, 1953
AlwNI	CAGNNNCTG	2 cut(s)	770, 1979
Ama87I	CYCGRG	1 cut(s)	422
ApaLI	GTGCAC	1 cut(s)	1732
ApeKI	GCWGC	9 cut(s)	131, 195, 367, 836, 839, 1193, 1676, 1805, 1973
ApoI	RAATTY	5 cut(s)	458, 790, 905, 1548, 1785
ArsI	GACNNNNNNTTYG	2 cut(s)	1027, 1059
Asp718I	GGTACC	1 cut(s)	1838
AspA2I	CCTAGG	1 cut(s)	1001
AspLEI	GCGC	1 cut(s)	145
AspS9I	GGNCC	5 cut(s)	91, 175, 207, 320, 731
AsuC2I	CCSGG	2 cut(s)	11, 327
AsuHPI	GGTGA	6 cut(s)	487, 612, 947, 1061, 1313, 1930
AsuNHI	GCTAGC	1 cut(s)	352
AvaI	CYCGRG	1 cut(s)	422
AvaII	GGWCC	3 cut(s)	175, 207, 731
AvrII	CCTAGG	1 cut(s)	1001
BaeGI	GKGCMC	1 cut(s)	1736
BalI	TGGCCA	1 cut(s)	447
BanI	GGYRCC	2 cut(s)	1720, 1838
BauI	CACGAG	1 cut(s)	1660
Bbv12I	GWGCWC	3 cut(s)	1285, 1650, 1736
BbvCI	CCTCAGC	1 cut(s)	1998
BbvI	GCAGC	9 cut(s)	143, 207, 379, 823, 851, 1180, 1663, 1792, 1985
BccI	CCATC	3 cut(s)	1733, 1767, 2049
BceAI	ACGGC	3 cut(s)	272, 320, 334
BciT130I	CCWGG	3 cut(s)	65, 182, 750
BcnI	CCSGG	2 cut(s)	11, 327
BcoDI	GTCTC	3 cut(s)	266, 761, 2024
BfaI	CTAG	4 cut(s)	353, 632, 1002, 1668
BfmI	CTRYAG	3 cut(s)	567, 837, 2058
BglI	GCCNNNNNGGC	1 cut(s)	98
BglII	AGATCT	3 cut(s)	628, 799, 1262
BlnI	CCTAGG	1 cut(s)	1001
Bme1390I	CCNGG	5 cut(s)	11, 65, 182, 327, 750
Bme18I	GGWCC	3 cut(s)	175, 207, 731
BmeRI	GACNNNNNGTC	1 cut(s)	46
BmeT110I	CYCGRG	1 cut(s)	422
BmgT120I	GGNCC	5 cut(s)	91, 175, 207, 320, 731
BmiI	GGNNCC	6 cut(s)	71, 208, 1157, 1403, 1722, 1840
BmrFI	CCNGG	5 cut(s)	11, 65, 182, 327, 750
BmrI	ACTGGG	2 cut(s)	501, 1886
BmsI	GCATC	4 cut(s)	449, 1198, 1985, 2063
BmtI	GCTAGC	1 cut(s)	356
BmuI	ACTGGG	2 cut(s)	501, 1886
BpmI	CTGGAG	3 cut(s)	164, 173, 1350
Bpu10I	CCTNAGC	2 cut(s)	1062, 1998
BpuEI	CTTGAG	2 cut(s)	131, 401
BpuMI	CCSGG	2 cut(s)	11, 327
BsaAI	YACGTR	1 cut(s)	1653
BsaBI	GATNNNNATC	1 cut(s)	696
BsaHI	GRCGYC	2 cut(s)	120, 259
BsaJI	CCNNGG	9 cut(s)	9, 64, 423, 579, 748, 749, 775, 883, 1001
BsaWI	WCCGGW	1 cut(s)	209
BsaXI	ACNNNNNCTCC	2 cut(s)	1478, 1508
Bsc4I	CCNNNNNNNGG	7 cut(s)	79, 235, 884, 1233, 1613, 1717, 1848
Bse1I	ACTGG	4 cut(s)	496, 918, 1367, 1881
Bse3DI	GCAATG	1 cut(s)	126
Bse8I	GATNNNNATC	1 cut(s)	696
BseBI	CCWGG	3 cut(s)	65, 182, 750
BseDI	CCNNGG	9 cut(s)	9, 64, 423, 579, 748, 749, 775, 883, 1001
BseGI	GGATG	3 cut(s)	616, 1213, 1777
BseJI	GATNNNNATC	1 cut(s)	696
BseLI	CCNNNNNNNGG	7 cut(s)	79, 235, 884, 1233, 1613, 1717, 1848
BseMI	GCAATG	1 cut(s)	126
BseMII	CTCAG	5 cut(s)	857, 884, 1203, 1916, 1989
BseNI	ACTGG	4 cut(s)	496, 918, 1367, 1881
BseRI	GAGGAG	3 cut(s)	398, 867, 2023
BseSI	GKGCMC	1 cut(s)	1736
BseXI	GCAGC	9 cut(s)	143, 207, 379, 823, 851, 1180, 1663, 1792, 1985
BseYI	CCCAGC	2 cut(s)	1510, 2013
BsgI	GTGCAG	3 cut(s)	214, 1489, 2006
Bsh1285I	CGRYCG	1 cut(s)	168
BshNI	GGYRCC	2 cut(s)	1720, 1838
BsiEI	CGRYCG	1 cut(s)	168
BsiHKAI	GWGCWC	3 cut(s)	1285, 1650, 1736
BsiHKCI	CYCGRG	1 cut(s)	422
BsiSI	CCGG	3 cut(s)	11, 210, 327
BslFI	GGGAC	3 cut(s)	318, 508, 1949
BslI	CCNNNNNNNGG	7 cut(s)	79, 235, 884, 1233, 1613, 1717, 1848
BsmAI	GTCTC	3 cut(s)	266, 761, 2024
BsmBI	CGTCTC	2 cut(s)	266, 2024
BsmFI	GGGAC	3 cut(s)	318, 508, 1949
BsmI	GAATGC	2 cut(s)	145, 1391
BsoBI	CYCGRG	1 cut(s)	422
Bsp1286I	GDGCHC	3 cut(s)	1285, 1650, 1736
BspCNI	CTCAG	5 cut(s)	856, 885, 1204, 1917, 1990
BspHI	TCATGA	1 cut(s)	1318
BspLI	GGNNCC	6 cut(s)	71, 208, 1157, 1403, 1722, 1840
BspMAI	CTGCAG	3 cut(s)	571, 841, 2062
BspOI	GCTAGC	1 cut(s)	356
BspPI	GGATC	6 cut(s)	519, 561, 686, 739, 932, 1953
BspT107I	GGYRCC	2 cut(s)	1720, 1838
BsrBI	CCGCTC	1 cut(s)	509
BsrDI	GCAATG	1 cut(s)	126
BsrI	ACTGG	4 cut(s)	496, 918, 1367, 1881
BssECI	CCNNGG	9 cut(s)	9, 64, 423, 579, 748, 749, 775, 883, 1001
BssNI	GRCGYC	2 cut(s)	120, 259
BssSI	CACGAG	1 cut(s)	1660
BssT1I	CCWWGG	4 cut(s)	579, 775, 883, 1001
Bst2BI	CACGAG	1 cut(s)	1660
Bst2UI	CCWGG	3 cut(s)	65, 182, 750
Bst4CI	ACNGT	7 cut(s)	293, 309, 1149, 1608, 1689, 1738, 1900
Bst6I	CTCTTC	3 cut(s)	269, 1099, 1529
BstACI	GRCGYC	2 cut(s)	120, 259
BstBAI	YACGTR	1 cut(s)	1653
BstC8I	GCNNGC	6 cut(s)	81, 94, 323, 354, 1281, 1508
BstDEI	CTNAG	6 cut(s)	843, 893, 1062, 1212, 1925, 1998
BstENI	CCTNNNNNAGG	1 cut(s)	882
BstF5I	GGATG	3 cut(s)	616, 1213, 1777
BstHHI	GCGC	1 cut(s)	145
BstMAI	GTCTC	3 cut(s)	266, 761, 2024
BstMCI	CGRYCG	1 cut(s)	168
BstNI	CCWGG	3 cut(s)	65, 182, 750
BstNSI	RCATGY	1 cut(s)	394
BstSCI	CCNGG	5 cut(s)	9, 63, 180, 325, 748
BstSFI	CTRYAG	3 cut(s)	567, 837, 2058
BstSLI	GKGCMC	1 cut(s)	1736
BstV1I	GCAGC	9 cut(s)	143, 207, 379, 823, 851, 1180, 1663, 1792, 1985
BstX2I	RGATCY	5 cut(s)	553, 628, 799, 937, 1262
BstYI	RGATCY	5 cut(s)	553, 628, 799, 937, 1262
BtsCI	GGATG	3 cut(s)	616, 1213, 1777
BtsIMutI	CAGTG	3 cut(s)	462, 911, 1922
Cac8I	GCNNGC	6 cut(s)	81, 94, 323, 354, 1281, 1508
CaiI	CAGNNNCTG	2 cut(s)	770, 1979
CciI	TCATGA	1 cut(s)	1318
CfoI	GCGC	1 cut(s)	145
Cfr13I	GGNCC	5 cut(s)	91, 175, 207, 320, 731
CseI	GACGC	3 cut(s)	128, 248, 749
Csp6I	GTAC	3 cut(s)	1654, 1839, 1883
CviAII	CATG	4 cut(s)	391, 449, 1319, 1435
CviQI	GTAC	3 cut(s)	1654, 1839, 1883
DdeI	CTNAG	6 cut(s)	843, 893, 1062, 1212, 1925, 1998
DraIII	CACNNNGTG	1 cut(s)	192
DriI	GACNNNNNGTC	1 cut(s)	46
EaeI	YGGCCR	3 cut(s)	3, 445, 1621
Eam1104I	CTCTTC	3 cut(s)	269, 1099, 1529
Eam1105I	GACNNNNNGTC	1 cut(s)	46
EarI	CTCTTC	3 cut(s)	269, 1099, 1529
Eco130I	CCWWGG	4 cut(s)	579, 775, 883, 1001
Eco147I	AGGCCT	2 cut(s)	888, 1666
Eco32I	GATATC	1 cut(s)	873
Eco47I	GGWCC	3 cut(s)	175, 207, 731
Eco57I	CTGAAG	3 cut(s)	455, 542, 645
Eco88I	CYCGRG	1 cut(s)	422
EcoNI	CCTNNNNNAGG	1 cut(s)	882
EcoO109I	RGGNCCY	1 cut(s)	175
EcoRI	GAATTC	1 cut(s)	905
EcoRII	CCWGG	3 cut(s)	63, 180, 748
EcoRV	GATATC	1 cut(s)	873
EcoT14I	CCWWGG	4 cut(s)	579, 775, 883, 1001
ErhI	CCWWGG	4 cut(s)	579, 775, 883, 1001
Esp3I	CGTCTC	2 cut(s)	266, 2024
FaeI	CATG	4 cut(s)	394, 452, 1322, 1438
FaqI	GGGAC	3 cut(s)	318, 508, 1949
FatI	CATG	4 cut(s)	390, 448, 1318, 1434
FauI	CCCGC	4 cut(s)	72, 101, 295, 330
FokI	GGATG	3 cut(s)	623, 1220, 1784
FspBI	CTAG	4 cut(s)	353, 632, 1002, 1668
GlaI	GCGC	1 cut(s)	144
GsaI	CCCAGC	2 cut(s)	1514, 2017
GsuI	CTGGAG	3 cut(s)	164, 173, 1350
HapII	CCGG	3 cut(s)	11, 210, 327
HgaI	GACGC	3 cut(s)	128, 248, 749
HhaI	GCGC	1 cut(s)	145
Hin1I	GRCGYC	2 cut(s)	120, 259
Hin1II	CATG	4 cut(s)	394, 452, 1322, 1438
Hin6I	GCGC	1 cut(s)	143
HinP1I	GCGC	1 cut(s)	143
HincII	GTYRAC	1 cut(s)	1075
HindII	GTYRAC	1 cut(s)	1075
HindIII	AAGCTT	3 cut(s)	159, 542, 855
HinfI	GANTC	8 cut(s)	17, 679, 1025, 1052, 1430, 1475, 1812, 1904
HpaII	CCGG	3 cut(s)	11, 210, 327
HphI	GGTGA	6 cut(s)	487, 612, 947, 1061, 1313, 1930
Hpy166II	GTNNAC	4 cut(s)	966, 1075, 1734, 1883
Hpy188I	TCNGA	9 cut(s)	52, 112, 138, 435, 691, 846, 894, 1160, 1755
Hpy188III	TCNNGA	6 cut(s)	169, 380, 764, 902, 1319, 1331
Hpy8I	GTNNAC	4 cut(s)	966, 1075, 1734, 1883
HpyAV	CCTTC	9 cut(s)	82, 166, 197, 430, 517, 568, 899, 1456, 1602
HpyCH4III	ACNGT	7 cut(s)	293, 309, 1149, 1608, 1689, 1738, 1900
HpyCH4IV	ACGT	3 cut(s)	1500, 1652, 2033
HpyF3I	CTNAG	6 cut(s)	843, 893, 1062, 1212, 1925, 1998
HpySE526I	ACGT	3 cut(s)	1500, 1652, 2033
Hsp92I	GRCGYC	2 cut(s)	120, 259
Hsp92II	CATG	4 cut(s)	394, 452, 1322, 1438
HspAI	GCGC	1 cut(s)	143
KpnI	GGTACC	1 cut(s)	1842
LmnI	GCTCC	4 cut(s)	27, 69, 583, 2010
Lsp1109I	GCAGC	9 cut(s)	143, 207, 379, 823, 851, 1180, 1663, 1792, 1985
LweI	GCATC	4 cut(s)	449, 1198, 1985, 2063
MaeI	CTAG	4 cut(s)	353, 632, 1002, 1668
MaeII	ACGT	3 cut(s)	1500, 1652, 2033
MaeIII	GTNAC	4 cut(s)	212, 415, 1522, 1918
MbiI	CCGCTC	1 cut(s)	509
MboII	GAAGA	6 cut(s)	26, 256, 638, 688, 1116, 1546
MfeI	CAATTG	1 cut(s)	726
MflI	RGATCY	5 cut(s)	553, 628, 799, 937, 1262
MhlI	GDGCHC	3 cut(s)	1285, 1650, 1736
MlsI	TGGCCA	1 cut(s)	447
MluNI	TGGCCA	1 cut(s)	447
MlyI	GAGTC	2 cut(s)	1439, 1484
MmeI	TCCRAC	6 cut(s)	30, 90, 588, 963, 1183, 1407
Mox20I	TGGCCA	1 cut(s)	447
MscI	TGGCCA	1 cut(s)	447
MseI	TTAA	4 cut(s)	588, 645, 1151, 1530
MslI	CAYNNNNRTG	2 cut(s)	785, 1764
Msp20I	TGGCCA	1 cut(s)	447
MspA1I	CMGCKG	1 cut(s)	198
MspI	CCGG	3 cut(s)	11, 210, 327
MspR9I	CCNGG	5 cut(s)	11, 65, 182, 327, 750
MunI	CAATTG	1 cut(s)	726
Mva1269I	GAATGC	2 cut(s)	145, 1391
MvaI	CCWGG	3 cut(s)	65, 182, 750
NciI	CCSGG	2 cut(s)	11, 327
NheI	GCTAGC	1 cut(s)	352
NlaIII	CATG	4 cut(s)	394, 452, 1322, 1438
NlaIV	GGNNCC	6 cut(s)	71, 208, 1157, 1403, 1722, 1840
NmuCI	GTSAC	2 cut(s)	415, 1918
NspI	RCATGY	1 cut(s)	394
PagI	TCATGA	1 cut(s)	1318
PasI	CCCWGGG	1 cut(s)	749
PceI	AGGCCT	2 cut(s)	888, 1666
PcsI	WCGNNNNNNNCGW	1 cut(s)	1658
PctI	GAATGC	2 cut(s)	145, 1391
PfeI	GAWTC	6 cut(s)	17, 679, 1025, 1052, 1812, 1904
Ple19I	CGATCG	1 cut(s)	168
PleI	GAGTC	2 cut(s)	1438, 1483
PpsI	GAGTC	2 cut(s)	1438, 1483
Ppu21I	YACGTR	1 cut(s)	1653
PpuMI	RGGWCCY	1 cut(s)	175
PsiI	TTATAA	1 cut(s)	1361
Psp5II	RGGWCCY	1 cut(s)	175
Psp6I	CCWGG	3 cut(s)	63, 180, 748
PspFI	CCCAGC	2 cut(s)	1510, 2013
PspGI	CCWGG	3 cut(s)	63, 180, 748
PspN4I	GGNNCC	6 cut(s)	71, 208, 1157, 1403, 1722, 1840
PspPI	GGNCC	5 cut(s)	91, 175, 207, 320, 731
PspPPI	RGGWCCY	1 cut(s)	175
PsrI	GAACNNNNNNTAC	2 cut(s)	1866, 1898
PstI	CTGCAG	3 cut(s)	571, 841, 2062
PstNI	CAGNNNCTG	2 cut(s)	770, 1979
PsuI	RGATCY	5 cut(s)	553, 628, 799, 937, 1262
PvuI	CGATCG	1 cut(s)	168
RsaI	GTAC	3 cut(s)	1655, 1840, 1884
RsaNI	GTAC	3 cut(s)	1654, 1839, 1883
RseI	CAYNNNNRTG	2 cut(s)	785, 1764
SaqAI	TTAA	4 cut(s)	588, 645, 1151, 1530
Sau96I	GGNCC	5 cut(s)	91, 175, 207, 320, 731
SchI	GAGTC	2 cut(s)	1439, 1484
ScrFI	CCNGG	5 cut(s)	11, 65, 182, 327, 750
SduI	GDGCHC	3 cut(s)	1285, 1650, 1736
SfaNI	GCATC	4 cut(s)	449, 1198, 1985, 2063
SfcI	CTRYAG	3 cut(s)	567, 837, 2058
SinI	GGWCC	3 cut(s)	175, 207, 731
SmiMI	CAYNNNNRTG	2 cut(s)	785, 1764
SmlI	CTYRAG	2 cut(s)	146, 380
SmoI	CTYRAG	2 cut(s)	146, 380
SseBI	AGGCCT	2 cut(s)	888, 1666
SspMI	CTAG	4 cut(s)	353, 632, 1002, 1668
StuI	AGGCCT	2 cut(s)	888, 1666
StyD4I	CCNGG	5 cut(s)	9, 63, 180, 325, 748
StyI	CCWWGG	4 cut(s)	579, 775, 883, 1001
TaaI	ACNGT	7 cut(s)	293, 309, 1149, 1608, 1689, 1738, 1900
TaiI	ACGT	3 cut(s)	1503, 1655, 2036
TaqI	TCGA	6 cut(s)	164, 168, 242, 453, 1844, 1864
TauI	GCSGC	4 cut(s)	8, 125, 201, 367
TfiI	GAWTC	6 cut(s)	17, 679, 1025, 1052, 1812, 1904
Tru1I	TTAA	4 cut(s)	588, 645, 1151, 1530
Tru9I	TTAA	4 cut(s)	588, 645, 1151, 1530
TscAI	CASTG	3 cut(s)	469, 918, 1929
TseFI	GTSAC	2 cut(s)	415, 1918
TseI	GCWGC	9 cut(s)	131, 195, 367, 836, 839, 1193, 1676, 1805, 1973
Tsp45I	GTSAC	2 cut(s)	415, 1918
TspDTI	ATGAA	6 cut(s)	1087, 1095, 1191, 1335, 1451, 1887
TspGWI	ACGGA	6 cut(s)	14, 218, 723, 1031, 1304, 1943
TspRI	CASTG	3 cut(s)	469, 918, 1929
VneI	GTGCAC	1 cut(s)	1732
VpaK11BI	GGWCC	3 cut(s)	175, 207, 731
XagI	CCTNNNNNAGG	1 cut(s)	882
XapI	RAATTY	5 cut(s)	458, 790, 905, 1548, 1785
XceI	RCATGY	1 cut(s)	394
XmaJI	CCTAGG	1 cut(s)	1001
XspI	CTAG	4 cut(s)	353, 632, 1002, 1668

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.