Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Contig00940

Orthogroup ID

OG0001310

Physical Location & Seq

Genomic Coordinates Reverse (-)

8158 .. 10690

Gene Structure 2533 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw0G020620.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1641 bp

ATGAGTGATAATACAGAAAGCTCCCGCCGTGCTCTGGGCAACACTCTTGAACGGCGCTTCTTTTACATTCCTTCTTTCAAGATTTACGGCGCTGTTGCTGGCCTCTACGACTATGGCTCGCCCGGCTGCAAACTCAAGAAGAATGTCCTTTCACTTTGGCATCAGGTTAGGGTTAACTTAACCAATTCTCTGCATCTGGCCATGTATATAAGTTCACTGATCTCTATGGTTAAGGATAAGAAGAAAGGGACTTGCTACAAGGCTGATCACTTGCTCAAGGATTTCTGCAATAAGAAGCTTGAAGAGGACCGTCACATTAGTTCAGAGATGGCAATGGAGCTGAAAGAAGTGCTTTCGAAATTGGACGGTCTATCAGCTGAAGAGCTGGGTGCATGCATCAAGAAATATGGGATTACAGCCCCTGAAACAGATAACCATCCTCTCTCAGATCCTTATCCTTTCAATTTGATGTTTCAAACATCAATTGGTCCGTCTGGTTCGAGTACTGGTTTCTTTCGCCCAGAAACTGCACAAGGCATATTTGTGAATTTTATGGACATTTACTATGACAATGGGTGCAAGCTGCCTTTTGCTGTTGCGCAAGTTGGCCAGGCTTTTCGAAACGAGATATCTCCTCGCCAAGGCCTGTTGAGAGTCCGTGAGTTCACACTCGCGGAGATTGAGCACTTTGTTGATCCTAAAGACAAATCTCACCTGAAATTCCCCAGCGTTGAGAAGTTGGAACTTCTGATGTTCCCAAGGGATGAACAAAAGCCTGGGCAATCTGCAAGATTAATCAAAGTGGGTGAAGCGGTGCATTCTAAGATTGTGGATAATGAAACTCTTGGCTACTTCATTGGGAAAGTATATCTCTTCCTCACTCGTCTGGGTATAGACAAGAACCGTTTGAGGTTCAGGCAACACCTTGCTAAAGAAATGGCTCACTATGCTACTTCCTATGGCTGGATTGAGTGTGTCGGTATTGCAGATAGATCTGCATATGACTTGAAGGCTCATTCGGGGAAAAGTCGTTTTGAGGCACATGAACAACTTTCAGAACCAAGAGACGTGGAGAAATTGGTTATATCTCCGGTAAAGAAACAGATTGGCCCTGCTTTGGGGAAGAAGCAAAAGAATGTGCTTGAAGCTTTGGAGGCTATGAATGAAGAAGAAATTTCAAAGTTGATGGCTGAGTTAGACTCAAAAGGGGAGGTGGACTTCTTTGTCTGCACTCTTGGGGAACATGTATCTATTAAGAGCAGTATGGTGACAATTTCAAAGGTTAAAACAAGAGAACATCAAAGAAAATTCACACCCTCAGTAATCGAGCCTTCATTTGGTATTGGGCGGATTATATATTGTCTCTTTGAGCATTCCTTCTACACCAGACCAGATAAAACTGGGGATGAACAATTGAATGTGTTTTGCTTTAACCCTCTTGTGGCGCCTTTTAAGTGCATGGTTTTCCCTCTGGTTCGGAACCAACAATTTGAGGCTGTTGCTAAGGACATTTCCAAGTCATTGACTGCTGCTGGCATATCGCATAAAATGGATACTACAGGTACCTCGATTGGGAAACGTTATGCACGTACTGATGAGCTCGGTGTACCCTTTGCGATAATGGTTGATTCTACATCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0003674 GO:0003824 GO:0004812 GO:0004820 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006426 GO:0006518 GO:0006520 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009117 GO:0009165 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0012505 GO:0015959 GO:0015960 GO:0015965 GO:0015966 GO:0016070 GO:0016874 GO:0016875 GO:0018130 GO:0019438 GO:0019538 GO:0019637 GO:0019752 GO:0031410 GO:0031982 GO:0031984 GO:0032543 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046686 GO:0046983 GO:0050896 GO:0055086 GO:0070127 GO:0070150 GO:0071704 GO:0090304 GO:0090407 GO:0097708 GO:0098791 GO:0140053 GO:0140098 GO:0140101 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon WHEP-TRS tRNA-synt_2b

Protein Analysis

546

Amino Acids

61.57

Weight (kDa)

8.67

Isoelectric Point (pI)

37.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2b	PF00587	173 - 458	2.7e-08	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	485 - 544	9.6e-12	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29880
fragaria_vesca	FvH4_4g22441 FvH4_4g22450 FvH4_4g22450 FvH4_4g22610 FvH4_4g22610
malus_domestica	MD13G1131000.v1.1 MD13G1145800.v1.1 MD16G1141200.v1.1
prunus_persica	Prupe.1G214600_v2.0.a1
pyrus_communis	pycom13g11430 pycom13g12530 pycom16g11160 pycom16g11170
rosa_chinensis	RchiOBHm_Chr1g0361881 RchiOBHm_Chr4g0428711 RchiOBHm_Chr4g0428851
rosa_laevigata	RLG00000007120 RLG00000007133 RLG00000027689 RLG00000027690 RLG00000027694
rosa_multiflora	Rmu_co8208336.1_g000001 Rmu_co8210234.1_g000001 Rmu_sc0003316.1_g000006 Rmu_sc0008612.1_g000003 Rmu_sc0018216.1_g000001 Rmu_ssc0000124.1_g000013
rosa_roxburghii	Rroxscaffold_4G00294630 Rroxscaffold_5G00370530
rosa_rugosa	Rorug01G0292100 Rorug04G0227400 Rorug04G0227500 Rorug04G0229700
rosa_samantha	Rh1AG302800 Rh1BG265900 Rh1BG266000 Rh1CG284000 Rh1DG295900 Rh1DG296000 Rh4AG284700 Rh4AG284900 Rh4AG286400 Rh4BG290700 Rh4BG290900 Rh4BG292200 Rh4CG308200 Rh4DG288200 Rh4DG289400
rosa_wichuraiana	Rw0G020620 Rw1G026830 Rw4G024720 Rw4G024830 Rw7G040280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1223
Acc16I	TGCGCA	1 cut(s)	600
Acc65I	GGTACC	1 cut(s)	1562
AccB1I	GGYRCC	2 cut(s)	1444, 1562
AccII	CGCG	1 cut(s)	674
AciI	CCGC	4 cut(s)	25, 674, 812, 1348
AclI	AACGTT	1 cut(s)	1579
AclWI	GGATC	2 cut(s)	443, 689
AcoI	YGGCCR	2 cut(s)	198, 607
AcsI	RAATTY	4 cut(s)	547, 719, 1173, 1307
AcuI	CTGAAG	1 cut(s)	399
AcyI	GRCGYC	1 cut(s)	1445
AfaI	GTAC	4 cut(s)	505, 1564, 1591, 1608
AfiI	CCNNNNNNNGG	8 cut(s)	34, 641, 963, 1117, 1118, 1337, 1441, 1572
AflIII	ACRYGT	1 cut(s)	1243
AjiI	CACGTC	1 cut(s)	1069
AjnI	CCWGG	2 cut(s)	609, 775
AluBI	AGCT	8 cut(s)	21, 298, 340, 377, 385, 583, 1148, 1600
AluI	AGCT	8 cut(s)	21, 298, 340, 377, 385, 583, 1148, 1600
Alw21I	GWGCWC	3 cut(s)	34, 687, 1602
Alw26I	GTCTC	2 cut(s)	1059, 1367
AlwI	GGATC	2 cut(s)	443, 689
AlwNI	CAGNNNCTG	2 cut(s)	422, 527
AoxI	GGCC	5 cut(s)	100, 198, 607, 643, 1108
ApeKI	GCWGC	3 cut(s)	126, 583, 1529
ApoI	RAATTY	4 cut(s)	547, 719, 1173, 1307
AseI	ATTAAT	1 cut(s)	794
Asp718I	GGTACC	1 cut(s)	1562
AspLEI	GCGC	4 cut(s)	57, 92, 601, 1447
AspS9I	GGNCC	3 cut(s)	307, 488, 1109
AsuC2I	CCSGG	1 cut(s)	123
AsuHPI	GGTGA	3 cut(s)	704, 818, 1279
AsuII	TTCGAA	2 cut(s)	356, 619
AvaII	GGWCC	2 cut(s)	307, 488
BalI	TGGCCA	2 cut(s)	200, 609
BanI	GGYRCC	2 cut(s)	1444, 1562
BanII	GRGCYC	1 cut(s)	1602
Bbv12I	GWGCWC	3 cut(s)	34, 687, 1602
BbvI	GCAGC	3 cut(s)	113, 570, 1516
BccI	CCATC	3 cut(s)	322, 444, 1180
BceAI	ACGGC	3 cut(s)	12, 68, 103
BciT130I	CCWGG	2 cut(s)	611, 777
BciVI	GTATCC	1 cut(s)	1546
BclI	TGATCA	1 cut(s)	265
BcnI	CCSGG	1 cut(s)	123
BcoDI	GTCTC	2 cut(s)	1059, 1367
BfaI	CTAG	1 cut(s)	1639
BfmI	CTRYAG	1 cut(s)	1557
BfoI	RGCGCY	3 cut(s)	58, 93, 1448
BfuI	GTATCC	1 cut(s)	1546
BglII	AGATCT	1 cut(s)	992
BisI	GCNGC	3 cut(s)	127, 584, 1530
BlsI	GCNGC	3 cut(s)	128, 585, 1531
BmcAI	AGTACT	1 cut(s)	505
Bme1390I	CCNGG	3 cut(s)	123, 611, 777
Bme18I	GGWCC	2 cut(s)	307, 488
BmgBI	CACGTC	1 cut(s)	1069
BmgT120I	GGNCC	3 cut(s)	307, 488, 1109
BmiI	GGNNCC	3 cut(s)	1446, 1481, 1564
BmrFI	CCNGG	3 cut(s)	123, 611, 777
BmrI	ACTGGG	1 cut(s)	1410
BmsI	GCATC	3 cut(s)	169, 202, 405
BmuI	ACTGGG	1 cut(s)	1410
BplI	GAGNNNNNCTC	2 cut(s)	1184, 1216
Bpu10I	CCTNAGC	1 cut(s)	1503
Bpu14I	TTCGAA	2 cut(s)	356, 619
BpuEI	CTTGAG	2 cut(s)	119, 260
BpuMI	CCSGG	1 cut(s)	123
BsaAI	YACGTR	1 cut(s)	1589
BsaBI	GATNNNNATC	2 cut(s)	435, 453
BsaHI	GRCGYC	1 cut(s)	1445
BsaJI	CCNNGG	3 cut(s)	640, 758, 776
BsaWI	WCCGGW	1 cut(s)	1090
Bsc4I	CCNNNNNNNGG	8 cut(s)	34, 641, 963, 1117, 1118, 1337, 1441, 1572
Bse1I	ACTGG	2 cut(s)	511, 1405
Bse3DI	GCAATG	1 cut(s)	339
Bse8I	GATNNNNATC	2 cut(s)	435, 453
BseBI	CCWGG	2 cut(s)	611, 777
BseDI	CCNNGG	3 cut(s)	640, 758, 776
BseGI	GGATG	4 cut(s)	436, 769, 1411, 1634
BseJI	GATNNNNATC	2 cut(s)	435, 453
BseLI	CCNNNNNNNGG	8 cut(s)	34, 641, 963, 1117, 1118, 1337, 1441, 1572
BseMI	GCAATG	1 cut(s)	339
BseMII	CTCAG	3 cut(s)	459, 1182, 1332
BseNI	ACTGG	2 cut(s)	511, 1405
BseRI	GAGGAG	1 cut(s)	624
BseXI	GCAGC	3 cut(s)	113, 570, 1516
BseYI	CCCAGC	2 cut(s)	385, 725
BsgI	GTGCAG	2 cut(s)	513, 1213
Bsh1236I	CGCG	1 cut(s)	674
BshFI	GGCC	5 cut(s)	102, 200, 609, 645, 1110
BshNI	GGYRCC	2 cut(s)	1444, 1562
BsiHKAI	GWGCWC	3 cut(s)	34, 687, 1602
BsiSI	CCGG	2 cut(s)	123, 1091
BslFI	GGGAC	1 cut(s)	262
BslI	CCNNNNNNNGG	8 cut(s)	34, 641, 963, 1117, 1118, 1337, 1441, 1572
BsmAI	GTCTC	2 cut(s)	1059, 1367
BsmBI	CGTCTC	1 cut(s)	1059
BsmFI	GGGAC	1 cut(s)	262
BsmI	GAATGC	2 cut(s)	817, 1372
BsnI	GGCC	5 cut(s)	102, 200, 609, 645, 1110
Bsp119I	TTCGAA	2 cut(s)	356, 619
Bsp1286I	GDGCHC	3 cut(s)	34, 687, 1602
Bsp143I	GATC	5 cut(s)	219, 265, 448, 694, 992
BspACI	CCGC	4 cut(s)	25, 674, 812, 1348
BspANI	GGCC	5 cut(s)	102, 200, 609, 645, 1110
BspCNI	CTCAG	3 cut(s)	458, 1183, 1331
BspFNI	CGCG	1 cut(s)	674
BspLI	GGNNCC	3 cut(s)	1446, 1481, 1564
BspPI	GGATC	2 cut(s)	443, 689
BspQI	GCTCTTC	1 cut(s)	375
BspT104I	TTCGAA	2 cut(s)	356, 619
BspT107I	GGYRCC	2 cut(s)	1444, 1562
BsrDI	GCAATG	1 cut(s)	339
BsrI	ACTGG	2 cut(s)	511, 1405
BssECI	CCNNGG	3 cut(s)	640, 758, 776
BssMI	GATC	5 cut(s)	219, 265, 448, 694, 992
BssNI	GRCGYC	1 cut(s)	1445
BssT1I	CCWWGG	2 cut(s)	640, 758
Bst2UI	CCWGG	2 cut(s)	611, 777
Bst4CI	ACNGT	3 cut(s)	311, 368, 905
Bst6I	CTCTTC	3 cut(s)	297, 375, 878
BstACI	GRCGYC	1 cut(s)	1445
BstBAI	YACGTR	1 cut(s)	1589
BstBI	TTCGAA	2 cut(s)	356, 619
BstC8I	GCNNGC	5 cut(s)	100, 119, 394, 581, 1534
BstDEI	CTNAG	5 cut(s)	445, 822, 1191, 1318, 1503
BstENI	CCTNNNNNAGG	1 cut(s)	639
BstF5I	GGATG	4 cut(s)	436, 769, 1411, 1634
BstFNI	CGCG	1 cut(s)	674
BstH2I	RGCGCY	3 cut(s)	58, 93, 1448
BstHHI	GCGC	4 cut(s)	57, 92, 601, 1447
BstKTI	GATC	5 cut(s)	222, 268, 451, 697, 995
BstMAI	GTCTC	2 cut(s)	1059, 1367
BstMBI	GATC	5 cut(s)	219, 265, 448, 694, 992
BstMWI	GCNNNNNNNGC	3 cut(s)	123, 947, 1154
BstNI	CCWGG	2 cut(s)	611, 777
BstNSI	RCATGY	2 cut(s)	396, 1247
BstSCI	CCNGG	3 cut(s)	121, 609, 775
BstSFI	CTRYAG	1 cut(s)	1557
BstUI	CGCG	1 cut(s)	674
BstV1I	GCAGC	3 cut(s)	113, 570, 1516
BstX2I	RGATCY	2 cut(s)	448, 992
BstYI	RGATCY	2 cut(s)	448, 992
BsuI	GTATCC	1 cut(s)	1546
BsuRI	GGCC	5 cut(s)	102, 200, 609, 645, 1110
BtrI	CACGTC	1 cut(s)	1069
BtsCI	GGATG	4 cut(s)	436, 769, 1411, 1634
BtsIMutI	CAGTG	1 cut(s)	215
Cac8I	GCNNGC	5 cut(s)	100, 119, 394, 581, 1534
CaiI	CAGNNNCTG	2 cut(s)	422, 527
CfoI	GCGC	4 cut(s)	57, 92, 601, 1447
Cfr13I	GGNCC	3 cut(s)	307, 488, 1109
Csp6I	GTAC	4 cut(s)	504, 1563, 1590, 1607
CspCI	CAANNNNNGTGG	2 cut(s)	1050, 1085
CviAII	CATG	5 cut(s)	202, 393, 1043, 1244, 1459
CviQI	GTAC	4 cut(s)	504, 1563, 1590, 1607
DdeI	CTNAG	5 cut(s)	445, 822, 1191, 1318, 1503
DinI	GGCGCC	1 cut(s)	1446
DpnI	GATC	5 cut(s)	221, 267, 450, 696, 994
DpnII	GATC	5 cut(s)	219, 265, 448, 694, 992
DrdI	GACNNNNNNGTC	1 cut(s)	1223
DseDI	GACNNNNNNGTC	1 cut(s)	1223
EaeI	YGGCCR	2 cut(s)	198, 607
Eam1104I	CTCTTC	3 cut(s)	297, 375, 878
EarI	CTCTTC	3 cut(s)	297, 375, 878
EciI	GGCGGA	1 cut(s)	1363
Ecl136II	GAGCTC	1 cut(s)	1600
Eco130I	CCWWGG	2 cut(s)	640, 758
Eco147I	AGGCCT	1 cut(s)	645
Eco24I	GRGCYC	1 cut(s)	1602
Eco32I	GATATC	1 cut(s)	630
Eco47I	GGWCC	2 cut(s)	307, 488
Eco53kI	GAGCTC	1 cut(s)	1600
Eco57I	CTGAAG	1 cut(s)	399
EcoICRI	GAGCTC	1 cut(s)	1600
EcoNI	CCTNNNNNAGG	1 cut(s)	639
EcoRII	CCWGG	2 cut(s)	609, 775
EcoRV	GATATC	1 cut(s)	630
EcoT14I	CCWWGG	2 cut(s)	640, 758
EcoT22I	ATGCAT	1 cut(s)	398
EcoT38I	GRGCYC	1 cut(s)	1602
EgeI	GGCGCC	1 cut(s)	1446
EheI	GGCGCC	1 cut(s)	1446
ErhI	CCWWGG	2 cut(s)	640, 758
Esp3I	CGTCTC	1 cut(s)	1059
FaeI	CATG	5 cut(s)	205, 396, 1046, 1247, 1462
FalI	AAGNNNNNCTT	2 cut(s)	336, 368
FaqI	GGGAC	1 cut(s)	262
FatI	CATG	5 cut(s)	201, 392, 1042, 1243, 1458
FauI	CCCGC	1 cut(s)	32
FauNDI	CATATG	1 cut(s)	1000
FbaI	TGATCA	1 cut(s)	265
Fnu4HI	GCNGC	3 cut(s)	127, 584, 1530
FokI	GGATG	4 cut(s)	423, 776, 1418, 1621
FriOI	GRGCYC	1 cut(s)	1602
Fsp4HI	GCNGC	3 cut(s)	127, 584, 1530
FspBI	CTAG	1 cut(s)	1639
FspI	TGCGCA	1 cut(s)	600
GlaI	GCGC	4 cut(s)	56, 91, 600, 1446
GluI	GCNGC	3 cut(s)	127, 584, 1530
GsaI	CCCAGC	2 cut(s)	389, 729
HaeII	RGCGCY	3 cut(s)	58, 93, 1448
HaeIII	GGCC	5 cut(s)	102, 200, 609, 645, 1110
HapII	CCGG	2 cut(s)	123, 1091
HhaI	GCGC	4 cut(s)	57, 92, 601, 1447
Hin1I	GRCGYC	1 cut(s)	1445
Hin1II	CATG	5 cut(s)	205, 396, 1046, 1247, 1462
Hin6I	GCGC	4 cut(s)	55, 90, 599, 1445
HinP1I	GCGC	4 cut(s)	55, 90, 599, 1445
HincII	GTYRAC	1 cut(s)	175
HindII	GTYRAC	1 cut(s)	175
HindIII	AAGCTT	2 cut(s)	296, 1146
HinfI	GANTC	3 cut(s)	654, 1199, 1628
HpaI	GTTAAC	1 cut(s)	175
HpaII	CCGG	2 cut(s)	123, 1091
HphI	GGTGA	3 cut(s)	704, 818, 1279
Hpy166II	GTNNAC	5 cut(s)	175, 215, 666, 1216, 1607
Hpy188I	TCNGA	5 cut(s)	325, 448, 750, 1057, 1479
Hpy188III	TCNNGA	4 cut(s)	47, 79, 136, 400
Hpy8I	GTNNAC	5 cut(s)	175, 215, 666, 1216, 1607
HpyAV	CCTTC	4 cut(s)	81, 1003, 1341, 1387
HpyCH4III	ACNGT	3 cut(s)	311, 368, 905
HpyCH4IV	ACGT	3 cut(s)	1068, 1579, 1588
HpyF10VI	GCNNNNNNNGC	3 cut(s)	123, 947, 1154
HpyF3I	CTNAG	5 cut(s)	445, 822, 1191, 1318, 1503
HpySE526I	ACGT	3 cut(s)	1068, 1579, 1588
Hsp92I	GRCGYC	1 cut(s)	1445
Hsp92II	CATG	5 cut(s)	205, 396, 1046, 1247, 1462
HspAI	GCGC	4 cut(s)	55, 90, 599, 1445
KasI	GGCGCC	1 cut(s)	1444
KpnI	GGTACC	1 cut(s)	1566
Ksp22I	TGATCA	1 cut(s)	265
KspAI	GTTAAC	1 cut(s)	175
Kzo9I	GATC	5 cut(s)	219, 265, 448, 694, 992
LguI	GCTCTTC	1 cut(s)	375
LmnI	GCTCC	2 cut(s)	26, 337
Lsp1109I	GCAGC	3 cut(s)	113, 570, 1516
LweI	GCATC	3 cut(s)	169, 202, 405
MaeI	CTAG	1 cut(s)	1639
MaeII	ACGT	3 cut(s)	1068, 1579, 1588
MaeIII	GTNAC	2 cut(s)	311, 1267
MalI	GATC	5 cut(s)	221, 267, 450, 696, 994
MboI	GATC	5 cut(s)	219, 265, 448, 694, 992
MboII	GAAGA	8 cut(s)	151, 253, 314, 392, 865, 1135, 1178, 1181
MfeI	CAATTG	2 cut(s)	483, 1412
MflI	RGATCY	2 cut(s)	448, 992
MhlI	GDGCHC	3 cut(s)	34, 687, 1602
MlsI	TGGCCA	2 cut(s)	200, 609
MluNI	TGGCCA	2 cut(s)	200, 609
Mly113I	GGCGCC	1 cut(s)	1445
MlyI	GAGTC	2 cut(s)	663, 1193
MmeI	TCCRAC	1 cut(s)	720
Mox20I	TGGCCA	2 cut(s)	200, 609
Mph1103I	ATGCAT	1 cut(s)	398
MscI	TGGCCA	2 cut(s)	200, 609
MseI	TTAA	8 cut(s)	174, 179, 231, 794, 1254, 1284, 1431, 1452
MslI	CAYNNNNRTG	1 cut(s)	542
Msp20I	TGGCCA	2 cut(s)	200, 609
MspA1I	CMGCKG	1 cut(s)	377
MspI	CCGG	2 cut(s)	123, 1091
MspR9I	CCNGG	3 cut(s)	123, 611, 777
MunI	CAATTG	2 cut(s)	483, 1412
Mva1269I	GAATGC	2 cut(s)	817, 1372
MvaI	CCWGG	2 cut(s)	611, 777
MvnI	CGCG	1 cut(s)	674
MwoI	GCNNNNNNNGC	3 cut(s)	123, 947, 1154
NarI	GGCGCC	1 cut(s)	1445
NciI	CCSGG	1 cut(s)	123
NdeI	CATATG	1 cut(s)	1000
NdeII	GATC	5 cut(s)	219, 265, 448, 694, 992
NlaIII	CATG	5 cut(s)	205, 396, 1046, 1247, 1462
NlaIV	GGNNCC	3 cut(s)	1446, 1481, 1564
NmuCI	GTSAC	2 cut(s)	311, 1267
NsbI	TGCGCA	1 cut(s)	600
NsiI	ATGCAT	1 cut(s)	398
NspI	RCATGY	2 cut(s)	396, 1247
NspV	TTCGAA	2 cut(s)	356, 619
PaeI	GCATGC	1 cut(s)	396
PceI	AGGCCT	1 cut(s)	645
PciI	ACATGT	1 cut(s)	1243
PciSI	GCTCTTC	1 cut(s)	375
PcsI	WCGNNNNNNNCGW	1 cut(s)	1585
PctI	GAATGC	2 cut(s)	817, 1372
PfeI	GAWTC	1 cut(s)	1628
PkrI	GCNGC	3 cut(s)	128, 585, 1531
PleI	GAGTC	2 cut(s)	662, 1193
PluTI	GGCGCC	1 cut(s)	1448
PpsI	GAGTC	2 cut(s)	662, 1193
Ppu21I	YACGTR	1 cut(s)	1589
PscI	ACATGT	1 cut(s)	1243
PshBI	ATTAAT	1 cut(s)	794
Psp124BI	GAGCTC	1 cut(s)	1602
Psp1406I	AACGTT	1 cut(s)	1579
Psp6I	CCWGG	2 cut(s)	609, 775
PspFI	CCCAGC	2 cut(s)	385, 725
PspGI	CCWGG	2 cut(s)	609, 775
PspN4I	GGNNCC	3 cut(s)	1446, 1481, 1564
PspPI	GGNCC	3 cut(s)	307, 488, 1109
PstNI	CAGNNNCTG	2 cut(s)	422, 527
PsuI	RGATCY	2 cut(s)	448, 992
PvuII	CAGCTG	1 cut(s)	377
RsaI	GTAC	4 cut(s)	505, 1564, 1591, 1608
RsaNI	GTAC	4 cut(s)	504, 1563, 1590, 1607
RseI	CAYNNNNRTG	1 cut(s)	542
SacI	GAGCTC	1 cut(s)	1602
SapI	GCTCTTC	1 cut(s)	375
SaqAI	TTAA	8 cut(s)	174, 179, 231, 794, 1254, 1284, 1431, 1452
SatI	GCNGC	3 cut(s)	127, 584, 1530
Sau3AI	GATC	5 cut(s)	219, 265, 448, 694, 992
Sau96I	GGNCC	3 cut(s)	307, 488, 1109
ScaI	AGTACT	1 cut(s)	505
SchI	GAGTC	2 cut(s)	663, 1193
ScrFI	CCNGG	3 cut(s)	123, 611, 777
SduI	GDGCHC	3 cut(s)	34, 687, 1602
SfaNI	GCATC	3 cut(s)	169, 202, 405
SfcI	CTRYAG	1 cut(s)	1557
SfoI	GGCGCC	1 cut(s)	1446
SfuI	TTCGAA	2 cut(s)	356, 619
SinI	GGWCC	2 cut(s)	307, 488
SmiMI	CAYNNNNRTG	1 cut(s)	542
SmlI	CTYRAG	2 cut(s)	134, 275
SmoI	CTYRAG	2 cut(s)	134, 275
SphI	GCATGC	1 cut(s)	396
SseBI	AGGCCT	1 cut(s)	645
SsiI	CCGC	4 cut(s)	25, 674, 812, 1348
SspDI	GGCGCC	1 cut(s)	1444
SspMI	CTAG	1 cut(s)	1639
SstI	GAGCTC	1 cut(s)	1602
StuI	AGGCCT	1 cut(s)	645
StyD4I	CCNGG	3 cut(s)	121, 609, 775
StyI	CCWWGG	2 cut(s)	640, 758
TaaI	ACNGT	3 cut(s)	311, 368, 905
TaiI	ACGT	3 cut(s)	1071, 1582, 1591
TaqI	TCGA	5 cut(s)	356, 500, 619, 1326, 1568
TatI	WGTACW	1 cut(s)	503
TfiI	GAWTC	1 cut(s)	1628
Tru1I	TTAA	8 cut(s)	174, 179, 231, 794, 1254, 1284, 1431, 1452
Tru9I	TTAA	8 cut(s)	174, 179, 231, 794, 1254, 1284, 1431, 1452
TscAI	CASTG	1 cut(s)	222
TseFI	GTSAC	2 cut(s)	311, 1267
TseI	GCWGC	3 cut(s)	126, 583, 1529
Tsp45I	GTSAC	2 cut(s)	311, 1267
TspDTI	ATGAA	8 cut(s)	780, 844, 852, 1059, 1175, 1179, 1323, 1422
TspGWI	ACGGA	2 cut(s)	480, 647
TspRI	CASTG	1 cut(s)	222
VpaK11BI	GGWCC	2 cut(s)	307, 488
VspI	ATTAAT	1 cut(s)	794
XagI	CCTNNNNNAGG	1 cut(s)	639
XapI	RAATTY	4 cut(s)	547, 719, 1173, 1307
XceI	RCATGY	2 cut(s)	396, 1247
XspI	CTAG	1 cut(s)	1639
ZrmI	AGTACT	1 cut(s)	505
Zsp2I	ATGCAT	1 cut(s)	398

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.