MD13G1167400.v1.1

RING-like zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001780
Physical Location & Seq
Reverse (-)
13553373 .. 13556342
2970 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1167400.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGGATTCTCCGTCGTCCGTTTTGTCATCCGACGACGCCACAGCCGTGAAATGTTCCATCTGCATGGAGGGCGTCTCTGACAACTGTGGCCGCACTATCGTCAAGCTTCAGTGCTCTCACTTGTTTCACCTCGATTGCATTGGTTCTGCATTCAATGTAAAGGGTATCATGGAATGCCCTAATTGTCGCGAAATTGAGAATGGCAAATGGAGGTACTATGTATCTCGTAGCCCTGACCGTGAGCCCGACCTATCTGAGGACGAGATGGATTATGAGGACAGTCCTATAGAGTTCCTTGACATGGGGCTACAGGGAACTATGCTACGCCGTTTCCATCACGGTGAATGGGAAATGGGATGGTTATCGCCTACCCGTCACAAACCACATTTTGAAGCGGGTTTGCTTGACCCTTATTCGGTCTTTTCTGGTAATCCTTATACCTACAATCAATGGACTGGCCTTCCGGTACCCAGCGAGGTATTTCCTTCGAATCCTTCCAGGATTGACTGGAGACCCGAACCTTTAATTTGTGATAGCTGTACCAATTTGCTTTCAACCGGTGACTATACTGGTGGTGCTAATTGGTCTTTGTATCCATCGGCAACCTTGAGTTCCGGGATTGATTATATTGGCATGCCAAATGCACCATTTGTTCCTCTAGTTGTTGAGGGCGGTTATGAATCCCCATTTGGTTTCGGTTATCAAAGAAGCAATGATCCCCTTCATTCCAACTTCCGGCAGCAGCTGCCAACCAATTTCCCTTCACAAGTGGCGCACGCTTCCATGGGAAGATCAAATGACTTGATGCACTGGCTTTGGAAAGGGCAGACAACACCTTCACTTGGACAGACTGCAGACTCCATTCCATTTTCTGTGAACATGAACTCTAGCCTGTCATCAGCAACCAACGATCATCGCCATGTCCAGGAAGGAAATGGGGTCCAACCTCAACGACTGTTTCCAGAAGCCGCCGGTTCTTTAACTTCTTTTTTCCCCGACGTGAATCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

337

Amino Acids

37.38

Weight (kDa)

4.87

Isoelectric Point (pI)

54.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 17 - 63 3.1e-07 Ring finger domain
zf-RING_11 PF17123 18 - 47 1e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 466
AccB1I GGYRCC 1 cut(s) 466
AccII CGCG 1 cut(s) 189
AciI CCGC 4 cut(s) 91, 395, 672, 969
AclWI GGATC 1 cut(s) 710
AcoI YGGCCR 1 cut(s) 88
AcuI CTGAAG 1 cut(s) 92
AcyI GRCGYC 2 cut(s) 36, 72
AfaI GTAC 3 cut(s) 215, 468, 541
AfiI CCNNNNNNNGG 6 cut(s) 256, 301, 415, 735, 842, 925
AgeI ACCGGT 1 cut(s) 557
AgsI TTSAA 3 cut(s) 154, 392, 555
AjiI CACGTC 1 cut(s) 1000
AjnI CCWGG 2 cut(s) 497, 924
AjuI GAANNNNNNNTTGG 2 cut(s) 672, 704
AleI CACNNNNGTG 1 cut(s) 44
AluBI AGCT 3 cut(s) 106, 537, 745
AluI AGCT 3 cut(s) 106, 537, 745
Alw21I GWGCWC 1 cut(s) 116
Alw26I GTCTC 2 cut(s) 79, 505
AlwI GGATC 1 cut(s) 710
AlwNI CAGNNNCTG 1 cut(s) 745
AoxI GGCC 2 cut(s) 88, 457
ApeKI GCWGC 3 cut(s) 739, 742, 745
AsiGI ACCGGT 1 cut(s) 557
Asp718I GGTACC 1 cut(s) 466
AspLEI GCGC 1 cut(s) 775
AspS9I GGNCC 1 cut(s) 940
AsuC2I CCSGG 1 cut(s) 616
AsuHPI GGTGA 3 cut(s) 119, 353, 572
AsuII TTCGAA 1 cut(s) 488
AvaII GGWCC 1 cut(s) 940
BanI GGYRCC 1 cut(s) 466
BanII GRGCYC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 116
BbvI GCAGC 3 cut(s) 732, 751, 754
BccI CCATC 5 cut(s) 65, 259, 342, 351, 604
BceAI ACGGC 2 cut(s) 29, 312
BciT130I CCWGG 2 cut(s) 499, 926
BciVI GTATCC 1 cut(s) 603
BcnI CCSGG 1 cut(s) 616
BcoDI GTCTC 2 cut(s) 79, 505
BfaI CTAG 2 cut(s) 659, 888
BfmI CTRYAG 3 cut(s) 285, 308, 852
BfuI GTATCC 1 cut(s) 603
BisI GCNGC 5 cut(s) 91, 740, 743, 746, 969
BlsI GCNGC 5 cut(s) 92, 741, 744, 747, 970
Bme1390I CCNGG 3 cut(s) 499, 616, 926
Bme18I GGWCC 1 cut(s) 940
BmgBI CACGTC 1 cut(s) 1000
BmgT120I GGNCC 1 cut(s) 940
BmiI GGNNCC 2 cut(s) 468, 941
BmrFI CCNGG 3 cut(s) 499, 616, 926
BmsI GCATC 1 cut(s) 795
BplI GAGNNNNNCTC 2 cut(s) 59, 91
BpmI CTGGAG 1 cut(s) 529
Bpu14I TTCGAA 1 cut(s) 488
BpuEI CTTGAG 1 cut(s) 628
BpuMI CCSGG 1 cut(s) 616
BsaBI GATNNNNATC 1 cut(s) 361
BsaHI GRCGYC 2 cut(s) 36, 72
BsaI GGTCTC 1 cut(s) 505
BsaJI CCNNGG 1 cut(s) 783
BsaWI WCCGGW 2 cut(s) 463, 557
Bsc4I CCNNNNNNNGG 6 cut(s) 256, 301, 415, 735, 842, 925
Bse118I RCCGGY 2 cut(s) 557, 971
Bse1I ACTGG 4 cut(s) 460, 512, 574, 815
Bse3DI GCAATG 1 cut(s) 718
Bse8I GATNNNNATC 1 cut(s) 361
BseBI CCWGG 2 cut(s) 499, 926
BseDI CCNNGG 1 cut(s) 783
BseGI GGATG 2 cut(s) 26, 362
BseJI GATNNNNATC 1 cut(s) 361
BseLI CCNNNNNNNGG 6 cut(s) 256, 301, 415, 735, 842, 925
BseMI GCAATG 1 cut(s) 718
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 4 cut(s) 460, 512, 574, 815
BseXI GCAGC 3 cut(s) 732, 751, 754
BseYI CCCAGC 1 cut(s) 470
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 2 cut(s) 90, 459
BshNI GGYRCC 1 cut(s) 466
BshTI ACCGGT 1 cut(s) 557
BsiHKAI GWGCWC 1 cut(s) 116
BsiSI CCGG 5 cut(s) 464, 558, 615, 736, 972
BslI CCNNNNNNNGG 6 cut(s) 256, 301, 415, 735, 842, 925
BsmAI GTCTC 2 cut(s) 79, 505
BsmBI CGTCTC 1 cut(s) 79
BsmI GAATGC 2 cut(s) 149, 179
BsnI GGCC 2 cut(s) 90, 459
Bso31I GGTCTC 1 cut(s) 505
Bsp119I TTCGAA 1 cut(s) 488
Bsp1286I GDGCHC 2 cut(s) 116, 246
Bsp143I GATC 3 cut(s) 715, 791, 910
Bsp19I CCATGG 1 cut(s) 783
Bsp68I TCGCGA 1 cut(s) 189
BspACI CCGC 4 cut(s) 91, 395, 672, 969
BspANI GGCC 2 cut(s) 90, 459
BspCNI CTCAG 1 cut(s) 247
BspFNI CGCG 1 cut(s) 189
BspLI GGNNCC 2 cut(s) 468, 941
BspMAI CTGCAG 1 cut(s) 856
BspPI GGATC 1 cut(s) 710
BspT104I TTCGAA 1 cut(s) 488
BspT107I GGYRCC 1 cut(s) 466
BspTNI GGTCTC 1 cut(s) 505
BsrDI GCAATG 1 cut(s) 718
BsrFI RCCGGY 2 cut(s) 557, 971
BsrI ACTGG 4 cut(s) 460, 512, 574, 815
BssAI RCCGGY 2 cut(s) 557, 971
BssECI CCNNGG 1 cut(s) 783
BssMI GATC 3 cut(s) 715, 791, 910
BssNI GRCGYC 2 cut(s) 36, 72
BssT1I CCWWGG 1 cut(s) 783
Bst2UI CCWGG 2 cut(s) 499, 926
Bst4CI ACNGT 5 cut(s) 86, 239, 281, 341, 957
BstACI GRCGYC 2 cut(s) 36, 72
BstAPI GCANNNNNTGC 1 cut(s) 745
BstBI TTCGAA 1 cut(s) 488
BstC8I GCNNGC 2 cut(s) 635, 777
BstDEI CTNAG 1 cut(s) 255
BstDSI CCRYGG 1 cut(s) 783
BstENI CCTNNNNNAGG 1 cut(s) 254
BstF5I GGATG 2 cut(s) 26, 362
BstFNI CGCG 1 cut(s) 189
BstHHI GCGC 1 cut(s) 775
BstKTI GATC 3 cut(s) 718, 794, 913
BstMAI GTCTC 2 cut(s) 79, 505
BstMBI GATC 3 cut(s) 715, 791, 910
BstMWI GCNNNNNNNGC 2 cut(s) 69, 745
BstNI CCWGG 2 cut(s) 499, 926
BstNSI RCATGY 1 cut(s) 637
BstSCI CCNGG 3 cut(s) 497, 614, 924
BstSFI CTRYAG 3 cut(s) 285, 308, 852
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 3 cut(s) 732, 751, 754
BstXI CCANNNNNNTGG 1 cut(s) 64
BsuI GTATCC 1 cut(s) 603
BsuRI GGCC 2 cut(s) 90, 459
BtgI CCRYGG 1 cut(s) 783
BtgZI GCGATG 1 cut(s) 899
BtrI CACGTC 1 cut(s) 1000
BtsCI GGATG 2 cut(s) 26, 362
BtsIMutI CAGTG 2 cut(s) 116, 808
BtuMI TCGCGA 1 cut(s) 189
Cac8I GCNNGC 2 cut(s) 635, 777
CaiI CAGNNNCTG 1 cut(s) 745
CfoI GCGC 1 cut(s) 775
Cfr10I RCCGGY 2 cut(s) 557, 971
Cfr13I GGNCC 1 cut(s) 940
CseI GACGC 2 cut(s) 44, 61
Csp6I GTAC 3 cut(s) 214, 467, 540
CspAI ACCGGT 1 cut(s) 557
CviAII CATG 7 cut(s) 64, 169, 301, 634, 784, 880, 920
CviQI GTAC 3 cut(s) 214, 467, 540
DdeI CTNAG 1 cut(s) 255
DpnI GATC 3 cut(s) 717, 793, 912
DpnII GATC 3 cut(s) 715, 791, 910
EaeI YGGCCR 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 783
Eco24I GRGCYC 1 cut(s) 246
Eco31I GGTCTC 1 cut(s) 505
Eco47I GGWCC 1 cut(s) 940
Eco57I CTGAAG 1 cut(s) 92
EcoNI CCTNNNNNAGG 1 cut(s) 254
EcoRII CCWGG 2 cut(s) 497, 924
EcoT14I CCWWGG 1 cut(s) 783
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 783
Esp3I CGTCTC 1 cut(s) 79
FaeI CATG 7 cut(s) 67, 172, 304, 637, 787, 883, 923
FatI CATG 7 cut(s) 63, 168, 300, 633, 783, 879, 919
FauI CCCGC 1 cut(s) 388
Fnu4HI GCNGC 5 cut(s) 91, 740, 743, 746, 969
FokI GGATG 2 cut(s) 13, 369
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 5 cut(s) 91, 740, 743, 746, 969
FspBI CTAG 2 cut(s) 659, 888
GlaI GCGC 1 cut(s) 774
GluI GCNGC 5 cut(s) 91, 740, 743, 746, 969
GsaI CCCAGC 1 cut(s) 474
GsuI CTGGAG 1 cut(s) 529
HaeIII GGCC 2 cut(s) 90, 459
HapII CCGG 5 cut(s) 464, 558, 615, 736, 972
HgaI GACGC 2 cut(s) 44, 61
HhaI GCGC 1 cut(s) 775
Hin1I GRCGYC 2 cut(s) 36, 72
Hin1II CATG 7 cut(s) 67, 172, 304, 637, 787, 883, 923
Hin6I GCGC 1 cut(s) 773
HinP1I GCGC 1 cut(s) 773
HindIII AAGCTT 1 cut(s) 104
HinfI GANTC 5 cut(s) 5, 490, 680, 857, 1003
HpaII CCGG 5 cut(s) 464, 558, 615, 736, 972
HphI GGTGA 3 cut(s) 119, 353, 572
Hpy166II GTNNAC 1 cut(s) 877
Hpy188I TCNGA 3 cut(s) 31, 79, 256
Hpy188III TCNNGA 2 cut(s) 188, 962
Hpy8I GTNNAC 1 cut(s) 877
Hpy99I CGWCG 4 cut(s) 16, 35, 38, 1001
HpyAV CCTTC 7 cut(s) 470, 495, 504, 731, 771, 846, 923
HpyCH4III ACNGT 5 cut(s) 86, 239, 281, 341, 957
HpyCH4IV ACGT 1 cut(s) 999
HpyCH4V TGCA 6 cut(s) 63, 138, 149, 644, 808, 854
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 745
HpyF3I CTNAG 1 cut(s) 255
HpySE526I ACGT 1 cut(s) 999
Hsp92I GRCGYC 2 cut(s) 36, 72
Hsp92II CATG 7 cut(s) 67, 172, 304, 637, 787, 883, 923
HspAI GCGC 1 cut(s) 773
KpnI GGTACC 1 cut(s) 470
Kzo9I GATC 3 cut(s) 715, 791, 910
Lsp1109I GCAGC 3 cut(s) 732, 751, 754
LweI GCATC 1 cut(s) 795
MaeI CTAG 2 cut(s) 659, 888
MaeII ACGT 1 cut(s) 999
MaeIII GTNAC 2 cut(s) 374, 560
MalI GATC 3 cut(s) 717, 793, 912
MboI GATC 3 cut(s) 715, 791, 910
MboII GAAGA 1 cut(s) 801
MhlI GDGCHC 2 cut(s) 116, 246
MluCI AATT 6 cut(s) 181, 192, 525, 544, 580, 754
MlyI GAGTC 1 cut(s) 851
MmeI TCCRAC 3 cut(s) 54, 753, 967
MnlI CCTC 9 cut(s) 61, 140, 204, 250, 268, 469, 661, 666, 957
MseI TTAA 2 cut(s) 524, 980
MslI CAYNNNNRTG 4 cut(s) 44, 62, 339, 918
MspA1I CMGCKG 1 cut(s) 745
MspI CCGG 5 cut(s) 464, 558, 615, 736, 972
MspR9I CCNGG 3 cut(s) 499, 616, 926
Mva1269I GAATGC 2 cut(s) 149, 179
MvaI CCWGG 2 cut(s) 499, 926
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 2 cut(s) 69, 745
NciI CCSGG 1 cut(s) 616
NcoI CCATGG 1 cut(s) 783
NdeII GATC 3 cut(s) 715, 791, 910
NlaIII CATG 7 cut(s) 67, 172, 304, 637, 787, 883, 923
NlaIV GGNNCC 2 cut(s) 468, 941
NmuCI GTSAC 2 cut(s) 374, 560
NruI TCGCGA 1 cut(s) 189
NspI RCATGY 1 cut(s) 637
NspV TTCGAA 1 cut(s) 488
OliI CACNNNNGTG 1 cut(s) 44
PaeI GCATGC 1 cut(s) 637
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PctI GAATGC 2 cut(s) 149, 179
PfeI GAWTC 4 cut(s) 5, 490, 680, 1003
PfoI TCCNGGA 3 cut(s) 497, 614, 924
PinAI ACCGGT 1 cut(s) 557
PkrI GCNGC 5 cut(s) 92, 741, 744, 747, 970
PleI GAGTC 1 cut(s) 851
PpsI GAGTC 1 cut(s) 851
Psp6I CCWGG 2 cut(s) 497, 924
PspFI CCCAGC 1 cut(s) 470
PspGI CCWGG 2 cut(s) 497, 924
PspN4I GGNNCC 2 cut(s) 468, 941
PspPI GGNCC 1 cut(s) 940
PstI CTGCAG 1 cut(s) 856
PstNI CAGNNNCTG 1 cut(s) 745
PvuII CAGCTG 1 cut(s) 745
RruI TCGCGA 1 cut(s) 189
RsaI GTAC 3 cut(s) 215, 468, 541
RsaNI GTAC 3 cut(s) 214, 467, 540
RseI CAYNNNNRTG 4 cut(s) 44, 62, 339, 918
SaqAI TTAA 2 cut(s) 524, 980
SatI GCNGC 5 cut(s) 91, 740, 743, 746, 969
Sau3AI GATC 3 cut(s) 715, 791, 910
Sau96I GGNCC 1 cut(s) 940
SchI GAGTC 1 cut(s) 851
ScrFI CCNGG 3 cut(s) 499, 616, 926
SduI GDGCHC 2 cut(s) 116, 246
SfaNI GCATC 1 cut(s) 795
SfcI CTRYAG 3 cut(s) 285, 308, 852
SfuI TTCGAA 1 cut(s) 488
SinI GGWCC 1 cut(s) 940
SmiMI CAYNNNNRTG 4 cut(s) 44, 62, 339, 918
SmlI CTYRAG 1 cut(s) 607
SmoI CTYRAG 1 cut(s) 607
SphI GCATGC 1 cut(s) 637
Sse9I AATT 6 cut(s) 181, 192, 525, 544, 580, 754
SsiI CCGC 4 cut(s) 91, 395, 672, 969
SspMI CTAG 2 cut(s) 659, 888
StyD4I CCNGG 3 cut(s) 497, 614, 924
StyI CCWWGG 1 cut(s) 783
TaaI ACNGT 5 cut(s) 86, 239, 281, 341, 957
TaiI ACGT 1 cut(s) 1002
TaqI TCGA 2 cut(s) 132, 488
TaqII GACCGA 1 cut(s) 406
TasI AATT 6 cut(s) 181, 192, 525, 544, 580, 754
TauI GCSGC 2 cut(s) 93, 971
TfiI GAWTC 4 cut(s) 5, 490, 680, 1003
Tru1I TTAA 2 cut(s) 524, 980
Tru9I TTAA 2 cut(s) 524, 980
TscAI CASTG 2 cut(s) 116, 815
TseFI GTSAC 2 cut(s) 374, 560
TseI GCWGC 3 cut(s) 739, 742, 745
Tsp45I GTSAC 2 cut(s) 374, 560
TspDTI ATGAA 3 cut(s) 693, 713, 896
TspGWI ACGGA 1 cut(s) 7
TspRI CASTG 2 cut(s) 116, 815
VpaK11BI GGWCC 1 cut(s) 940
XagI CCTNNNNNAGG 1 cut(s) 254
XceI RCATGY 1 cut(s) 637
XcmI CCANNNNNNNNNTGG 1 cut(s) 932
XspI CTAG 2 cut(s) 659, 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.