Rh2DG399500
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001780
Physical Location & Seq
Reverse (-)
58165805 .. 58192908
27104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG399500.1

Sequence Viewer

Length: 702 bp
ATGGTGGAAAAAAGTGGAAAAGGTTGGCTTGCCTTCAAAGAAACTTTCCTTGCAATCGTGGCCCTGGAATCTGTAAATTATAATTTTGGGATTAATTGTGGAGGACCTGAGATTACGTCTTCCTCTAATAGGATTGTGTATGAGAAGGAAAATGAGCCCCTTGGACCAGCTTCATATTTTGTGACTGGCACAAGCAGATGGGGAGTTAGCAATGTCGGATATTTCGCTGGCAGTACCAATCCTCAGTATACAAGTTTCTCCTTATCTCAATTCACAAATACTTTAGATTCTGTGCTATTCCAGACTGCAAGGCTCTCTGCTTCATCAATTAGATACTTTGGCTTGGGGCTTGAGACTGGTAACTACACCGTGAACCTCAAGTTTGCAGAACATGTCATTCTGGCTGCTACTACCGGGAAATTTCTTGGGAGACGTCTTTTTGATATTTATATCCAGACAAAGGAAACTTCACGAAAGCATACCAATAGTGACTTCAATAGTAGCAAGACACATATCCCCAAATCTTCAAAAGTTAAACCGGAGTCTGTAGTAGTGAAAGAGAAAAGCGTGTCAGTGATTCTTTTGGCTAGAGGAAAGGGTAAAAGGATGGGTGGAGGTGCCATCGACAATTGGTTAGGGCGCCGGTGGTGGCAGGAGGTGTTGCATGTTGTTAGAGAACAACGTCGCGTTCAAGTATACTAG

Protein Analysis

233

Amino Acids

26.02

Weight (kDa)

9.83

Isoelectric Point (pI)

29.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 29 - 200 8.1e-21 Malectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 81
AatII GACGTC 1 cut(s) 436
AccB1I GGYRCC 2 cut(s) 617, 639
AccI GTMKAC 2 cut(s) 248, 696
AccII CGCG 1 cut(s) 687
AcsI RAATTY 1 cut(s) 419
AcyI GRCGYC 2 cut(s) 433, 640
AfaI GTAC 1 cut(s) 235
AfiI CCNNNNNNNGG 2 cut(s) 129, 460
AflIII ACRYGT 1 cut(s) 391
AgsI TTSAA 4 cut(s) 37, 496, 528, 692
AjnI CCWGG 1 cut(s) 63
AjuI GAANNNNNNNTTGG 2 cut(s) 476, 508
AluBI AGCT 1 cut(s) 170
AluI AGCT 1 cut(s) 170
Alw26I GTCTC 2 cut(s) 347, 424
AoxI GGCC 1 cut(s) 60
ApeKI GCWGC 1 cut(s) 404
ApoI RAATTY 1 cut(s) 419
AseI ATTAAT 1 cut(s) 93
AspLEI GCGC 1 cut(s) 642
AspS9I GGNCC 3 cut(s) 61, 104, 164
AsuC2I CCSGG 1 cut(s) 415
AvaII GGWCC 2 cut(s) 104, 164
BanI GGYRCC 2 cut(s) 617, 639
BanII GRGCYC 1 cut(s) 159
BbsI GAAGAC 1 cut(s) 111
BbvI GCAGC 1 cut(s) 391
BccI CCATC 3 cut(s) 192, 601, 629
BciT130I CCWGG 1 cut(s) 65
BcnI CCSGG 1 cut(s) 415
BcoDI GTCTC 2 cut(s) 347, 424
BfaI CTAG 2 cut(s) 588, 700
BfmI CTRYAG 1 cut(s) 546
BfoI RGCGCY 1 cut(s) 643
BisI GCNGC 1 cut(s) 405
BlsI GCNGC 1 cut(s) 406
Bme1390I CCNGG 2 cut(s) 65, 415
Bme18I GGWCC 2 cut(s) 104, 164
BmgT120I GGNCC 3 cut(s) 61, 104, 164
BmiI GGNNCC 2 cut(s) 619, 641
BmrFI CCNGG 2 cut(s) 65, 415
BpiI GAAGAC 1 cut(s) 111
BpuEI CTTGAG 2 cut(s) 362, 371
BpuMI CCSGG 1 cut(s) 415
BsaHI GRCGYC 2 cut(s) 433, 640
BsaJI CCNNGG 2 cut(s) 63, 160
BsaWI WCCGGW 1 cut(s) 538
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 460
Bse118I RCCGGY 1 cut(s) 642
Bse1I ACTGG 2 cut(s) 190, 361
Bse3DI GCAATG 1 cut(s) 217
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 2 cut(s) 63, 160
BseGI GGATG 1 cut(s) 612
BseLI CCNNNNNNNGG 2 cut(s) 129, 460
BseMI GCAATG 1 cut(s) 217
BseMII CTCAG 2 cut(s) 99, 257
BseNI ACTGG 2 cut(s) 190, 361
BseXI GCAGC 1 cut(s) 391
Bsh1236I CGCG 1 cut(s) 687
BshFI GGCC 1 cut(s) 62
BshNI GGYRCC 2 cut(s) 617, 639
BsiSI CCGG 3 cut(s) 414, 539, 643
BslI CCNNNNNNNGG 2 cut(s) 129, 460
BsmAI GTCTC 2 cut(s) 347, 424
BsmBI CGTCTC 1 cut(s) 424
BsnI GGCC 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 159
BspANI GGCC 1 cut(s) 62
BspCNI CTCAG 2 cut(s) 100, 256
BspFNI CGCG 1 cut(s) 687
BspLI GGNNCC 2 cut(s) 619, 641
BspT107I GGYRCC 2 cut(s) 617, 639
BsrDI GCAATG 1 cut(s) 217
BsrFI RCCGGY 1 cut(s) 642
BsrI ACTGG 2 cut(s) 190, 361
BssAI RCCGGY 1 cut(s) 642
BssECI CCNNGG 2 cut(s) 63, 160
BssNAI GTATAC 2 cut(s) 249, 697
BssNI GRCGYC 2 cut(s) 433, 640
BssT1I CCWWGG 1 cut(s) 160
Bst1107I GTATAC 2 cut(s) 249, 697
Bst2UI CCWGG 1 cut(s) 65
Bst4CI ACNGT 1 cut(s) 370
BstACI GRCGYC 2 cut(s) 433, 640
BstC8I GCNNGC 2 cut(s) 30, 229
BstDEI CTNAG 2 cut(s) 108, 243
BstENI CCTNNNNNAGG 1 cut(s) 127
BstF5I GGATG 1 cut(s) 612
BstFNI CGCG 1 cut(s) 687
BstH2I RGCGCY 1 cut(s) 643
BstHHI GCGC 1 cut(s) 642
BstMAI GTCTC 2 cut(s) 347, 424
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstNI CCWGG 1 cut(s) 65
BstNSI RCATGY 2 cut(s) 395, 668
BstSCI CCNGG 2 cut(s) 63, 413
BstSFI CTRYAG 1 cut(s) 546
BstUI CGCG 1 cut(s) 687
BstV1I GCAGC 1 cut(s) 391
BstV2I GAAGAC 1 cut(s) 111
BstZ17I GTATAC 2 cut(s) 249, 697
BsuRI GGCC 1 cut(s) 62
BtsCI GGATG 1 cut(s) 612
BtsIMutI CAGTG 1 cut(s) 579
Cac8I GCNNGC 2 cut(s) 30, 229
CfoI GCGC 1 cut(s) 642
Cfr10I RCCGGY 1 cut(s) 642
Cfr13I GGNCC 3 cut(s) 61, 104, 164
Csp6I GTAC 1 cut(s) 234
CviAII CATG 2 cut(s) 392, 665
CviJI RGCY 9 cut(s) 28, 62, 157, 170, 313, 342, 349, 404, 587
CviKI_1 RGCY 9 cut(s) 28, 62, 157, 170, 313, 342, 349, 404, 587
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 2 cut(s) 108, 243
DinI GGCGCC 1 cut(s) 641
Eco130I CCWWGG 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 159
Eco47I GGWCC 2 cut(s) 104, 164
EcoNI CCTNNNNNAGG 1 cut(s) 127
EcoO109I RGGNCCY 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 63
EcoT14I CCWWGG 1 cut(s) 160
EcoT38I GRGCYC 1 cut(s) 159
EgeI GGCGCC 1 cut(s) 641
EheI GGCGCC 1 cut(s) 641
ErhI CCWWGG 1 cut(s) 160
Esp3I CGTCTC 1 cut(s) 424
FaeI CATG 2 cut(s) 395, 668
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FatI CATG 2 cut(s) 391, 664
FblI GTMKAC 2 cut(s) 248, 696
Fnu4HI GCNGC 1 cut(s) 405
FokI GGATG 1 cut(s) 619
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 1 cut(s) 405
FspBI CTAG 2 cut(s) 588, 700
GlaI GCGC 1 cut(s) 641
GluI GCNGC 1 cut(s) 405
HaeII RGCGCY 1 cut(s) 643
HaeIII GGCC 1 cut(s) 62
HapII CCGG 3 cut(s) 414, 539, 643
HhaI GCGC 1 cut(s) 642
Hin1I GRCGYC 2 cut(s) 433, 640
Hin1II CATG 2 cut(s) 395, 668
Hin6I GCGC 1 cut(s) 640
HinP1I GCGC 1 cut(s) 640
HinfI GANTC 4 cut(s) 68, 287, 542, 577
HpaII CCGG 3 cut(s) 414, 539, 643
Hpy166II GTNNAC 3 cut(s) 249, 373, 697
Hpy188I TCNGA 1 cut(s) 218
Hpy188III TCNNGA 3 cut(s) 301, 454, 471
Hpy8I GTNNAC 3 cut(s) 249, 373, 697
Hpy99I CGWCG 1 cut(s) 687
HpyAV CCTTC 2 cut(s) 43, 139
HpyCH4III ACNGT 1 cut(s) 370
HpyCH4IV ACGT 3 cut(s) 116, 433, 682
HpyCH4V TGCA 4 cut(s) 53, 308, 386, 664
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
HpyF3I CTNAG 2 cut(s) 108, 243
HpySE526I ACGT 3 cut(s) 116, 433, 682
Hsp92I GRCGYC 2 cut(s) 433, 640
Hsp92II CATG 2 cut(s) 395, 668
HspAI GCGC 1 cut(s) 640
KasI GGCGCC 1 cut(s) 639
Lsp1109I GCAGC 1 cut(s) 391
MaeI CTAG 2 cut(s) 588, 700
MaeII ACGT 3 cut(s) 116, 433, 682
MaeIII GTNAC 3 cut(s) 181, 359, 488
MboII GAAGA 2 cut(s) 111, 516
MfeI CAATTG 1 cut(s) 628
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 7 cut(s) 76, 82, 94, 269, 327, 419, 628
Mly113I GGCGCC 1 cut(s) 640
MlyI GAGTC 1 cut(s) 551
MmeI TCCRAC 1 cut(s) 196
MnlI CCTC 7 cut(s) 95, 133, 252, 386, 584, 608, 649
MseI TTAA 2 cut(s) 93, 534
MspI CCGG 3 cut(s) 414, 539, 643
MspR9I CCNGG 2 cut(s) 65, 415
MunI CAATTG 1 cut(s) 628
MvaI CCWGG 1 cut(s) 65
MvnI CGCG 1 cut(s) 687
MwoI GCNNNNNNNGC 1 cut(s) 59
NarI GGCGCC 1 cut(s) 640
NciI CCSGG 1 cut(s) 415
NlaIII CATG 2 cut(s) 395, 668
NlaIV GGNNCC 2 cut(s) 619, 641
NmuCI GTSAC 2 cut(s) 181, 488
NspI RCATGY 2 cut(s) 395, 668
PciI ACATGT 1 cut(s) 391
PfeI GAWTC 3 cut(s) 68, 287, 577
PkrI GCNGC 1 cut(s) 406
PleI GAGTC 1 cut(s) 550
PluTI GGCGCC 1 cut(s) 643
PpsI GAGTC 1 cut(s) 550
PpuMI RGGWCCY 1 cut(s) 104
PscI ACATGT 1 cut(s) 391
PshBI ATTAAT 1 cut(s) 93
PsiI TTATAA 1 cut(s) 81
Psp5II RGGWCCY 1 cut(s) 104
Psp6I CCWGG 1 cut(s) 63
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 2 cut(s) 619, 641
PspPI GGNCC 3 cut(s) 61, 104, 164
PspPPI RGGWCCY 1 cut(s) 104
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
SaqAI TTAA 2 cut(s) 93, 534
SatI GCNGC 1 cut(s) 405
Sau96I GGNCC 3 cut(s) 61, 104, 164
SchI GAGTC 1 cut(s) 551
ScrFI CCNGG 2 cut(s) 65, 415
SduI GDGCHC 1 cut(s) 159
SetI ASST 9 cut(s) 25, 109, 119, 172, 378, 436, 619, 660, 685
SfcI CTRYAG 1 cut(s) 546
SfoI GGCGCC 1 cut(s) 641
SgrAI CRCCGGYG 1 cut(s) 642
SinI GGWCC 2 cut(s) 104, 164
SmlI CTYRAG 2 cut(s) 350, 377
SmoI CTYRAG 2 cut(s) 350, 377
Sse9I AATT 7 cut(s) 76, 82, 94, 269, 327, 419, 628
SspDI GGCGCC 1 cut(s) 639
SspMI CTAG 2 cut(s) 588, 700
StyD4I CCNGG 2 cut(s) 63, 413
StyI CCWWGG 1 cut(s) 160
TaaI ACNGT 1 cut(s) 370
TaiI ACGT 3 cut(s) 119, 436, 685
TaqI TCGA 1 cut(s) 624
TasI AATT 7 cut(s) 76, 82, 94, 269, 327, 419, 628
TfiI GAWTC 3 cut(s) 68, 287, 577
Tru1I TTAA 2 cut(s) 93, 534
Tru9I TTAA 2 cut(s) 93, 534
TscAI CASTG 1 cut(s) 579
TseFI GTSAC 2 cut(s) 181, 488
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 2 cut(s) 181, 488
TspDTI ATGAA 2 cut(s) 162, 312
TspRI CASTG 1 cut(s) 579
VpaK11BI GGWCC 2 cut(s) 104, 164
VspI ATTAAT 1 cut(s) 93
XagI CCTNNNNNAGG 1 cut(s) 127
XapI RAATTY 1 cut(s) 419
XceI RCATGY 2 cut(s) 395, 668
XmiI GTMKAC 2 cut(s) 248, 696
XspI CTAG 2 cut(s) 588, 700
ZraI GACGTC 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.