MD16G1167800.v1.1

RING-like zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001780
Physical Location & Seq
Forward (+)
13971156 .. 13973567
2412 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1167800.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGGAGGGTGTCTCCGACGACTGTGGCCGCACAGTCGTCAAGCTTCAGTGCTCTGACTTGTTTCACCTTGATTGCATTGGTTCTGCATTCAATGTAAAGGGTATCATGGAATGCCCTATCTGCGGCGAGATTGAGGATGGCGTATGGAGGTATTTTATATCTGGTAGCCCTGACCGCGAGCTCAACGTATCTGAGGAAGAGATGGATTATGAGGACAGTCCTATGGAGTTCTTTGACATGGGGTTACAGGGAACCATGTTACGCCATTTTCATCATGGTGAATGGGAAATGGGATGGTTACCGCCTACCCATCGCGGCTCAGAACCACATTTTGGAGGGAATTTGCTTGCCCCCTATTCGGTCTTTCCTGGCTATCCTTATACCAACAATCGATGGAATGGCCCTCCCATACCCAGCGAGGTATTGCCTTCGAATCCTTCCCAGATTTACTGGAGACCCGAACCTTTACTTTGTGATAGCTATGCCAATTTGTTTTCAACGGGCAGCTATACTGGTGGTGCTAATTGGTCTTCAGTTCCTTCCGCAACCTTGAGTTCCGGGGTTGATTTTACTGGCATGCCAAATGCACCATTTGTTCCTCAAATTGCTGACTGCGGCATGCGTTTTCAAACCAGTTATGAATCTCCATTTGGTCTTGCTTATCAAGGAAGCAATGATCCACTTCATCCCAACTTCCAAATGATGCAGCAGTCGCCAACCAATTTCCCTTCACAAGTGGCACAAGCTTCCATGGGAAGATCAAATGACGCGCCTTCACTTGGAAGTTGGGCAGGGCAGCCTAGCTTGATGGAAGTTGGAGTGTCCGTGCAAGTGCAACCTAGCTTGATGGACTGGCTTCGGGCAGGGCAGCCAGCACCTTCACTTGAACAGGCTGCAAACTCCAATTCATTTTTCGTCAACATGAACTCTGGCCTGTCGGAATCAGCAACCGACGGCCATCGCCTTGTGCAGGAAGGAAACGAGGTCCAACCATTCCATCTTTTTCCAGAAGACAGCGGTCCTTTGACTTATTTTTTCCCTGACGTGAATCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

352

Amino Acids

38.64

Weight (kDa)

4.36

Isoelectric Point (pI)

51.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 332
AccII CGCG 3 cut(s) 177, 315, 770
AciI CCGC 8 cut(s) 28, 123, 175, 302, 315, 543, 615, 1017
AclWI GGATC 1 cut(s) 671
AcoI YGGCCR 2 cut(s) 25, 955
AcsI RAATTY 1 cut(s) 340
AcuI CTGAAG 2 cut(s) 29, 516
AfiI CCNNNNNNNGG 5 cut(s) 122, 332, 358, 779, 970
AgsI TTSAA 4 cut(s) 91, 498, 629, 887
AjiI CACGTC 1 cut(s) 1045
AjnI CCWGG 1 cut(s) 367
AjuI GAANNNNNNNTTGG 6 cut(s) 315, 347, 633, 665, 896, 928
AluBI AGCT 7 cut(s) 43, 181, 480, 507, 746, 804, 843
AluI AGCT 7 cut(s) 43, 181, 480, 507, 746, 804, 843
Alw21I GWGCWC 2 cut(s) 53, 183
Alw26I GTCTC 2 cut(s) 16, 448
AlwI GGATC 1 cut(s) 671
AoxI GGCC 4 cut(s) 25, 400, 931, 955
ApeKI GCWGC 5 cut(s) 504, 706, 796, 868, 893
ApoI RAATTY 1 cut(s) 340
AspLEI GCGC 1 cut(s) 772
AspS9I GGNCC 3 cut(s) 401, 985, 1019
AsuC2I CCSGG 1 cut(s) 559
AsuHPI GGTGA 2 cut(s) 56, 290
AsuII TTCGAA 1 cut(s) 431
AvaII GGWCC 2 cut(s) 985, 1019
BanII GRGCYC 1 cut(s) 183
BbsI GAAGAC 2 cut(s) 522, 1017
Bbv12I GWGCWC 2 cut(s) 53, 183
BbvI GCAGC 5 cut(s) 516, 718, 808, 880, 880
BccI CCATC 9 cut(s) 131, 196, 288, 318, 387, 802, 841, 966, 1005
BceAI ACGGC 1 cut(s) 970
BciT130I CCWGG 1 cut(s) 369
BcnI CCSGG 1 cut(s) 559
BcoDI GTCTC 2 cut(s) 16, 448
BfaI CTAG 2 cut(s) 801, 840
BisI GCNGC 9 cut(s) 28, 124, 316, 505, 616, 707, 797, 869, 894
BlsI GCNGC 9 cut(s) 29, 125, 317, 506, 617, 708, 798, 870, 895
Bme1390I CCNGG 2 cut(s) 369, 559
Bme18I GGWCC 2 cut(s) 985, 1019
BmgBI CACGTC 1 cut(s) 1045
BmgT120I GGNCC 3 cut(s) 401, 985, 1019
BmiI GGNNCC 1 cut(s) 253
BmrFI CCNGG 2 cut(s) 369, 559
BmsI GCATC 1 cut(s) 693
BoxI GACNNNNGTC 1 cut(s) 1017
BpiI GAAGAC 2 cut(s) 522, 1017
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 472
Bpu14I TTCGAA 1 cut(s) 431
BpuEI CTTGAG 1 cut(s) 571
BpuMI CCSGG 1 cut(s) 559
Bsa29I ATCGAT 1 cut(s) 391
BsaI GGTCTC 1 cut(s) 448
BsaJI CCNNGG 2 cut(s) 558, 750
BsaXI ACNNNNNCTCC 2 cut(s) 810, 840
Bsc4I CCNNNNNNNGG 5 cut(s) 122, 332, 358, 779, 970
Bse1I ACTGG 5 cut(s) 455, 517, 577, 633, 857
Bse3DI GCAATG 1 cut(s) 679
BseBI CCWGG 1 cut(s) 369
BseCI ATCGAT 1 cut(s) 391
BseDI CCNNGG 2 cut(s) 558, 750
BseGI GGATG 3 cut(s) 142, 299, 685
BseLI CCNNNNNNNGG 5 cut(s) 122, 332, 358, 779, 970
BseMI GCAATG 1 cut(s) 679
BseMII CTCAG 2 cut(s) 183, 333
BseNI ACTGG 5 cut(s) 455, 517, 577, 633, 857
BseXI GCAGC 5 cut(s) 516, 718, 808, 880, 880
BseYI CCCAGC 1 cut(s) 413
BsgI GTGCAG 1 cut(s) 989
Bsh1236I CGCG 3 cut(s) 177, 315, 770
BshFI GGCC 4 cut(s) 27, 402, 933, 957
BshVI ATCGAT 1 cut(s) 391
BsiHKAI GWGCWC 2 cut(s) 53, 183
BsiSI CCGG 1 cut(s) 558
BslI CCNNNNNNNGG 5 cut(s) 122, 332, 358, 779, 970
BsmAI GTCTC 2 cut(s) 16, 448
BsmI GAATGC 2 cut(s) 86, 116
BsnI GGCC 4 cut(s) 27, 402, 933, 957
Bso31I GGTCTC 1 cut(s) 448
Bsp119I TTCGAA 1 cut(s) 431
Bsp1286I GDGCHC 2 cut(s) 53, 183
Bsp143I GATC 2 cut(s) 676, 758
Bsp19I CCATGG 1 cut(s) 750
BspACI CCGC 8 cut(s) 28, 123, 175, 302, 315, 543, 615, 1017
BspANI GGCC 4 cut(s) 27, 402, 933, 957
BspCNI CTCAG 2 cut(s) 184, 332
BspDI ATCGAT 1 cut(s) 391
BspFNI CGCG 3 cut(s) 177, 315, 770
BspLI GGNNCC 1 cut(s) 253
BspPI GGATC 1 cut(s) 671
BspT104I TTCGAA 1 cut(s) 431
BspTNI GGTCTC 1 cut(s) 448
BsrDI GCAATG 1 cut(s) 679
BsrI ACTGG 5 cut(s) 455, 517, 577, 633, 857
BssECI CCNNGG 2 cut(s) 558, 750
BssMI GATC 2 cut(s) 676, 758
BssT1I CCWWGG 1 cut(s) 750
Bst2UI CCWGG 1 cut(s) 369
Bst4CI ACNGT 3 cut(s) 23, 34, 218
Bst6I CTCTTC 1 cut(s) 192
BstBI TTCGAA 1 cut(s) 431
BstC8I GCNNGC 5 cut(s) 179, 348, 578, 620, 873
BstDEI CTNAG 2 cut(s) 192, 319
BstDSI CCRYGG 1 cut(s) 750
BstEII GGTNACC 1 cut(s) 297
BstENI CCTNNNNNAGG 1 cut(s) 968
BstF5I GGATG 3 cut(s) 142, 299, 685
BstFNI CGCG 3 cut(s) 177, 315, 770
BstHHI GCGC 1 cut(s) 772
BstKTI GATC 2 cut(s) 679, 761
BstMAI GTCTC 2 cut(s) 16, 448
BstMBI GATC 2 cut(s) 676, 758
BstMWI GCNNNNNNNGC 3 cut(s) 120, 174, 712
BstNI CCWGG 1 cut(s) 369
BstNSI RCATGY 2 cut(s) 580, 622
BstPAI GACNNNNGTC 1 cut(s) 1017
BstPI GGTNACC 1 cut(s) 297
BstSCI CCNGG 2 cut(s) 367, 557
BstUI CGCG 3 cut(s) 177, 315, 770
BstV1I GCAGC 5 cut(s) 516, 718, 808, 880, 880
BstV2I GAAGAC 2 cut(s) 522, 1017
Bsu15I ATCGAT 1 cut(s) 391
BsuRI GGCC 4 cut(s) 27, 402, 933, 957
BsuTUI ATCGAT 1 cut(s) 391
BtgI CCRYGG 1 cut(s) 750
BtgZI GCGATG 2 cut(s) 296, 944
BtrI CACGTC 1 cut(s) 1045
BtsCI GGATG 3 cut(s) 142, 299, 685
BtsIMutI CAGTG 1 cut(s) 53
Cac8I GCNNGC 5 cut(s) 179, 348, 578, 620, 873
CfoI GCGC 1 cut(s) 772
Cfr13I GGNCC 3 cut(s) 401, 985, 1019
ClaI ATCGAT 1 cut(s) 391
CseI GACGC 1 cut(s) 776
CviAII CATG 8 cut(s) 106, 238, 256, 275, 577, 619, 751, 922
DdeI CTNAG 2 cut(s) 192, 319
DpnI GATC 2 cut(s) 678, 760
DpnII GATC 2 cut(s) 676, 758
EaeI YGGCCR 2 cut(s) 25, 955
Eam1104I CTCTTC 1 cut(s) 192
EarI CTCTTC 1 cut(s) 192
Ecl136II GAGCTC 1 cut(s) 181
Eco130I CCWWGG 1 cut(s) 750
Eco24I GRGCYC 1 cut(s) 183
Eco31I GGTCTC 1 cut(s) 448
Eco47I GGWCC 2 cut(s) 985, 1019
Eco53kI GAGCTC 1 cut(s) 181
Eco57I CTGAAG 2 cut(s) 29, 516
Eco91I GGTNACC 1 cut(s) 297
EcoICRI GAGCTC 1 cut(s) 181
EcoNI CCTNNNNNAGG 1 cut(s) 968
EcoO65I GGTNACC 1 cut(s) 297
EcoRII CCWGG 1 cut(s) 367
EcoT14I CCWWGG 1 cut(s) 750
EcoT38I GRGCYC 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 750
FaeI CATG 8 cut(s) 109, 241, 259, 278, 580, 622, 754, 925
FatI CATG 8 cut(s) 105, 237, 255, 274, 576, 618, 750, 921
Fnu4HI GCNGC 9 cut(s) 28, 124, 316, 505, 616, 707, 797, 869, 894
FokI GGATG 3 cut(s) 149, 306, 672
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 9 cut(s) 28, 124, 316, 505, 616, 707, 797, 869, 894
FspBI CTAG 2 cut(s) 801, 840
GlaI GCGC 1 cut(s) 771
GluI GCNGC 9 cut(s) 28, 124, 316, 505, 616, 707, 797, 869, 894
GsaI CCCAGC 1 cut(s) 417
GsuI CTGGAG 1 cut(s) 472
HaeIII GGCC 4 cut(s) 27, 402, 933, 957
HapII CCGG 1 cut(s) 558
HgaI GACGC 1 cut(s) 776
HhaI GCGC 1 cut(s) 772
Hin1II CATG 8 cut(s) 109, 241, 259, 278, 580, 622, 754, 925
Hin6I GCGC 1 cut(s) 770
HinP1I GCGC 1 cut(s) 770
HincII GTYRAC 1 cut(s) 919
HindII GTYRAC 1 cut(s) 919
HindIII AAGCTT 2 cut(s) 41, 744
HinfI GANTC 4 cut(s) 433, 641, 941, 1048
HpaII CCGG 1 cut(s) 558
HphI GGTGA 2 cut(s) 56, 290
Hpy166II GTNNAC 1 cut(s) 919
Hpy188I TCNGA 5 cut(s) 16, 55, 193, 322, 940
Hpy188III TCNNGA 1 cut(s) 1007
Hpy8I GTNNAC 1 cut(s) 919
Hpy99I CGWCG 2 cut(s) 20, 956
HpyAV CCTTC 7 cut(s) 438, 447, 549, 738, 783, 888, 968
HpyCH4III ACNGT 3 cut(s) 23, 34, 218
HpyCH4IV ACGT 2 cut(s) 186, 1044
HpyCH4V TGCA 8 cut(s) 75, 86, 587, 706, 829, 835, 896, 970
HpyF10VI GCNNNNNNNGC 3 cut(s) 120, 174, 712
HpyF3I CTNAG 2 cut(s) 192, 319
HpySE526I ACGT 2 cut(s) 186, 1044
Hsp92II CATG 8 cut(s) 109, 241, 259, 278, 580, 622, 754, 925
HspAI GCGC 1 cut(s) 770
Kzo9I GATC 2 cut(s) 676, 758
Lsp1109I GCAGC 5 cut(s) 516, 718, 808, 880, 880
LweI GCATC 1 cut(s) 693
MaeI CTAG 2 cut(s) 801, 840
MaeII ACGT 2 cut(s) 186, 1044
MaeIII GTNAC 3 cut(s) 243, 258, 297
MalI GATC 2 cut(s) 678, 760
MboI GATC 2 cut(s) 676, 758
MboII GAAGA 4 cut(s) 209, 522, 768, 1022
MhlI GDGCHC 2 cut(s) 53, 183
MluCI AATT 6 cut(s) 340, 487, 523, 603, 721, 904
MmeI TCCRAC 4 cut(s) 39, 796, 918, 1012
MnlI CCTC 9 cut(s) 127, 141, 187, 205, 329, 412, 414, 609, 976
MslI CAYNNNNRTG 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 1017
MspI CCGG 1 cut(s) 558
MspR9I CCNGG 2 cut(s) 369, 559
Mva1269I GAATGC 2 cut(s) 86, 116
MvaI CCWGG 1 cut(s) 369
MvnI CGCG 3 cut(s) 177, 315, 770
MwoI GCNNNNNNNGC 3 cut(s) 120, 174, 712
NciI CCSGG 1 cut(s) 559
NcoI CCATGG 1 cut(s) 750
NdeII GATC 2 cut(s) 676, 758
NlaIII CATG 8 cut(s) 109, 241, 259, 278, 580, 622, 754, 925
NlaIV GGNNCC 1 cut(s) 253
NspI RCATGY 2 cut(s) 580, 622
NspV TTCGAA 1 cut(s) 431
PaeI GCATGC 2 cut(s) 580, 622
PctI GAATGC 2 cut(s) 86, 116
PfeI GAWTC 4 cut(s) 433, 641, 941, 1048
PflMI CCANNNNNTGG 1 cut(s) 332
PkrI GCNGC 9 cut(s) 29, 125, 317, 506, 617, 708, 798, 870, 895
PshAI GACNNNNGTC 1 cut(s) 1017
Psp124BI GAGCTC 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 367
PspEI GGTNACC 1 cut(s) 297
PspFI CCCAGC 1 cut(s) 413
PspGI CCWGG 1 cut(s) 367
PspN4I GGNNCC 1 cut(s) 253
PspPI GGNCC 3 cut(s) 401, 985, 1019
RseI CAYNNNNRTG 1 cut(s) 276
SacI GAGCTC 1 cut(s) 183
SatI GCNGC 9 cut(s) 28, 124, 316, 505, 616, 707, 797, 869, 894
Sau3AI GATC 2 cut(s) 676, 758
Sau96I GGNCC 3 cut(s) 401, 985, 1019
ScrFI CCNGG 2 cut(s) 369, 559
SduI GDGCHC 2 cut(s) 53, 183
SfaNI GCATC 1 cut(s) 693
SfuI TTCGAA 1 cut(s) 431
SinI GGWCC 2 cut(s) 985, 1019
SmiMI CAYNNNNRTG 1 cut(s) 276
SmlI CTYRAG 1 cut(s) 550
SmoI CTYRAG 1 cut(s) 550
SphI GCATGC 2 cut(s) 580, 622
Sse9I AATT 6 cut(s) 340, 487, 523, 603, 721, 904
SsiI CCGC 8 cut(s) 28, 123, 175, 302, 315, 543, 615, 1017
SspMI CTAG 2 cut(s) 801, 840
SstI GAGCTC 1 cut(s) 183
StyD4I CCNGG 2 cut(s) 367, 557
StyI CCWWGG 1 cut(s) 750
TaaI ACNGT 3 cut(s) 23, 34, 218
TaiI ACGT 2 cut(s) 189, 1047
TaqI TCGA 2 cut(s) 391, 431
TaqII GACCGA 1 cut(s) 349
TasI AATT 6 cut(s) 340, 487, 523, 603, 721, 904
TauI GCSGC 4 cut(s) 30, 126, 318, 618
TfiI GAWTC 4 cut(s) 433, 641, 941, 1048
TscAI CASTG 1 cut(s) 53
TseI GCWGC 5 cut(s) 504, 706, 796, 868, 893
TspDTI ATGAA 5 cut(s) 260, 654, 674, 897, 938
TspGWI ACGGA 1 cut(s) 814
TspRI CASTG 1 cut(s) 53
Van91I CCANNNNNTGG 1 cut(s) 332
VpaK11BI GGWCC 2 cut(s) 985, 1019
XagI CCTNNNNNAGG 1 cut(s) 968
XapI RAATTY 1 cut(s) 340
XceI RCATGY 2 cut(s) 580, 622
XcmI CCANNNNNNNNNTGG 1 cut(s) 272
XspI CTAG 2 cut(s) 801, 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.