Rh6AG317700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0001780
Physical Location & Seq
Reverse (-)
51680263 .. 51687715
7453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG317700.1

Sequence Viewer

Length: 453 bp
ATGTACAGACAACATAAAATTGCTGCAATATCAGAAGACCCATGGAAGCTCTCATCTTTATTGGATCACGGGTCAAAACTCTTTCTTGCATTTATCCTCTCGAGGTTTGACACTACTAGAGCTGATTCTAGTAGCAACATGATCCTTTGGAGACGATTATTTGAGACAGTTGATTTTCAACAATTAGAACCACAATGGCATCACTTTTCTGAGTTCATTCTCGATGAGGTAAGGGGGCTCTATGAGAAGGCCCAGGAGATATTGATGAAAGAAAGCAATGTTCAGATTTGTGATGATTGTATCAACCACCACCTTTCAAGTGGCCATGCTGGTCATAGTAATGGGGTTTCAGTTCCAGCTGGAAACCTGGGAGTTCCTAGCGCTGATGTTAATGCCATTCCCAGTGATTTCATAATTCCTCCTGCCGGTTTTGATGGTGTACGTACAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.86

Weight (kDa)

5.38

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 72, 136
AcoI YGGCCR 1 cut(s) 322
AfaI GTAC 3 cut(s) 5, 441, 445
AfeI AGCGCT 1 cut(s) 382
AfiI CCNNNNNNNGG 1 cut(s) 425
AgsI TTSAA 2 cut(s) 179, 318
AjnI CCWGG 2 cut(s) 252, 366
AluBI AGCT 3 cut(s) 49, 122, 359
AluI AGCT 3 cut(s) 49, 122, 359
Alw26I GTCTC 2 cut(s) 145, 158
AlwI GGATC 2 cut(s) 72, 136
Ama87I CYCGRG 1 cut(s) 100
Aor51HI AGCGCT 1 cut(s) 382
AoxI GGCC 2 cut(s) 249, 322
ApeKI GCWGC 1 cut(s) 23
AspLEI GCGC 1 cut(s) 383
AspS9I GGNCC 1 cut(s) 250
AvaI CYCGRG 1 cut(s) 100
BalI TGGCCA 1 cut(s) 324
BanII GRGCYC 1 cut(s) 240
BbsI GAAGAC 1 cut(s) 42
BbvI GCAGC 1 cut(s) 10
BccI CCATC 1 cut(s) 428
BciT130I CCWGG 2 cut(s) 254, 368
BcoDI GTCTC 2 cut(s) 145, 158
BfaI CTAG 3 cut(s) 117, 129, 378
BfoI RGCGCY 1 cut(s) 384
BisI GCNGC 1 cut(s) 24
BlsI GCNGC 1 cut(s) 25
Bme1390I CCNGG 2 cut(s) 254, 368
BmeT110I CYCGRG 1 cut(s) 100
BmgT120I GGNCC 1 cut(s) 250
BmrFI CCNGG 2 cut(s) 254, 368
BmrI ACTGGG 1 cut(s) 396
BmsI GCATC 1 cut(s) 208
BmuI ACTGGG 1 cut(s) 396
BpiI GAAGAC 1 cut(s) 42
BsaAI YACGTR 1 cut(s) 443
BsaJI CCNNGG 3 cut(s) 41, 252, 367
Bsc4I CCNNNNNNNGG 1 cut(s) 425
Bse118I RCCGGY 1 cut(s) 425
Bse1I ACTGG 1 cut(s) 402
Bse3DI GCAATG 1 cut(s) 283
BseBI CCWGG 2 cut(s) 254, 368
BseDI CCNNGG 3 cut(s) 41, 252, 367
BseLI CCNNNNNNNGG 1 cut(s) 425
BseMI GCAATG 1 cut(s) 283
BseMII CTCAG 1 cut(s) 201
BseNI ACTGG 1 cut(s) 402
BseXI GCAGC 1 cut(s) 10
BshFI GGCC 2 cut(s) 251, 324
BsiHKCI CYCGRG 1 cut(s) 100
BsiSI CCGG 1 cut(s) 426
BslI CCNNNNNNNGG 1 cut(s) 425
BsmAI GTCTC 2 cut(s) 145, 158
BsmBI CGTCTC 1 cut(s) 145
BsnI GGCC 2 cut(s) 251, 324
BsoBI CYCGRG 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 240
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 64, 141
Bsp19I CCATGG 1 cut(s) 41
BspANI GGCC 2 cut(s) 251, 324
BspCNI CTCAG 1 cut(s) 202
BspPI GGATC 2 cut(s) 72, 136
BsrDI GCAATG 1 cut(s) 283
BsrFI RCCGGY 1 cut(s) 425
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 402
BssAI RCCGGY 1 cut(s) 425
BssECI CCNNGG 3 cut(s) 41, 252, 367
BssMI GATC 2 cut(s) 64, 141
BssT1I CCWWGG 1 cut(s) 41
Bst2UI CCWGG 2 cut(s) 254, 368
Bst4CI ACNGT 1 cut(s) 169
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 443
BstDEI CTNAG 1 cut(s) 210
BstDSI CCRYGG 1 cut(s) 41
BstH2I RGCGCY 1 cut(s) 384
BstHHI GCGC 1 cut(s) 383
BstKTI GATC 2 cut(s) 67, 144
BstMAI GTCTC 2 cut(s) 145, 158
BstMBI GATC 2 cut(s) 64, 141
BstNI CCWGG 2 cut(s) 254, 368
BstSCI CCNGG 2 cut(s) 252, 366
BstSNI TACGTA 1 cut(s) 443
BstV1I GCAGC 1 cut(s) 10
BstV2I GAAGAC 1 cut(s) 42
BsuRI GGCC 2 cut(s) 251, 324
BtgI CCRYGG 1 cut(s) 41
BtsIMutI CAGTG 1 cut(s) 409
CfoI GCGC 1 cut(s) 383
Cfr10I RCCGGY 1 cut(s) 425
Cfr13I GGNCC 1 cut(s) 250
Csp6I GTAC 3 cut(s) 4, 440, 444
CviAII CATG 3 cut(s) 42, 139, 326
CviJI RGCY 6 cut(s) 49, 122, 238, 251, 324, 359
CviKI_1 RGCY 6 cut(s) 49, 122, 238, 251, 324, 359
CviQI GTAC 3 cut(s) 4, 440, 444
DdeI CTNAG 1 cut(s) 210
DpnI GATC 2 cut(s) 66, 143
DpnII GATC 2 cut(s) 64, 141
EaeI YGGCCR 1 cut(s) 322
Eco105I TACGTA 1 cut(s) 443
Eco130I CCWWGG 1 cut(s) 41
Eco24I GRGCYC 1 cut(s) 240
Eco47III AGCGCT 1 cut(s) 382
Eco88I CYCGRG 1 cut(s) 100
EcoRII CCWGG 2 cut(s) 252, 366
EcoT14I CCWWGG 1 cut(s) 41
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 41
Esp3I CGTCTC 1 cut(s) 145
FaeI CATG 3 cut(s) 45, 142, 329
FaiI YATR 7 cut(s) 15, 43, 140, 243, 327, 336, 413
FatI CATG 3 cut(s) 41, 138, 325
Fnu4HI GCNGC 1 cut(s) 24
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 1 cut(s) 24
FspBI CTAG 3 cut(s) 117, 129, 378
GlaI GCGC 1 cut(s) 382
GluI GCNGC 1 cut(s) 24
HaeII RGCGCY 1 cut(s) 384
HaeIII GGCC 2 cut(s) 251, 324
HapII CCGG 1 cut(s) 426
HhaI GCGC 1 cut(s) 383
Hin1II CATG 3 cut(s) 45, 142, 329
Hin6I GCGC 1 cut(s) 381
HinP1I GCGC 1 cut(s) 381
HinfI GANTC 1 cut(s) 125
HpaII CCGG 1 cut(s) 426
Hpy166II GTNNAC 1 cut(s) 440
Hpy188I TCNGA 3 cut(s) 34, 211, 285
Hpy188III TCNNGA 2 cut(s) 100, 221
Hpy8I GTNNAC 1 cut(s) 440
HpyAV CCTTC 1 cut(s) 241
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4IV ACGT 1 cut(s) 442
HpyCH4V TGCA 2 cut(s) 26, 89
HpyF3I CTNAG 1 cut(s) 210
HpySE526I ACGT 1 cut(s) 442
Hsp92II CATG 3 cut(s) 45, 142, 329
HspAI GCGC 1 cut(s) 381
Kzo9I GATC 2 cut(s) 64, 141
Lsp1109I GCAGC 1 cut(s) 10
LweI GCATC 1 cut(s) 208
MaeI CTAG 3 cut(s) 117, 129, 378
MaeII ACGT 1 cut(s) 442
MalI GATC 2 cut(s) 66, 143
MboI GATC 2 cut(s) 64, 141
MboII GAAGA 1 cut(s) 47
MhlI GDGCHC 1 cut(s) 240
MlsI TGGCCA 1 cut(s) 324
MluCI AATT 3 cut(s) 18, 182, 414
MluNI TGGCCA 1 cut(s) 324
MnlI CCTC 4 cut(s) 96, 107, 220, 429
Mox20I TGGCCA 1 cut(s) 324
MscI TGGCCA 1 cut(s) 324
MseI TTAA 1 cut(s) 390
MslI CAYNNNNRTG 1 cut(s) 339
Msp20I TGGCCA 1 cut(s) 324
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 1 cut(s) 426
MspR9I CCNGG 2 cut(s) 254, 368
MvaI CCWGG 2 cut(s) 254, 368
NcoI CCATGG 1 cut(s) 41
NdeII GATC 2 cut(s) 64, 141
NlaIII CATG 3 cut(s) 45, 142, 329
PaeR7I CTCGAG 1 cut(s) 100
PfeI GAWTC 1 cut(s) 125
PkrI GCNGC 1 cut(s) 25
Ppu21I YACGTR 1 cut(s) 443
Psp6I CCWGG 2 cut(s) 252, 366
PspGI CCWGG 2 cut(s) 252, 366
PspPI GGNCC 1 cut(s) 250
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 3 cut(s) 5, 441, 445
RsaNI GTAC 3 cut(s) 4, 440, 444
RseI CAYNNNNRTG 1 cut(s) 339
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 1 cut(s) 24
Sau3AI GATC 2 cut(s) 64, 141
Sau96I GGNCC 1 cut(s) 250
ScrFI CCNGG 2 cut(s) 254, 368
SduI GDGCHC 1 cut(s) 240
SetI ASST 8 cut(s) 51, 107, 124, 231, 315, 361, 369, 445
SfaNI GCATC 1 cut(s) 208
Sfr274I CTCGAG 1 cut(s) 100
SlaI CTCGAG 1 cut(s) 100
SmiMI CAYNNNNRTG 1 cut(s) 339
SmlI CTYRAG 1 cut(s) 100
SmoI CTYRAG 1 cut(s) 100
SnaBI TACGTA 1 cut(s) 443
Sse9I AATT 3 cut(s) 18, 182, 414
SspMI CTAG 3 cut(s) 117, 129, 378
StyD4I CCNGG 2 cut(s) 252, 366
StyI CCWWGG 1 cut(s) 41
TaaI ACNGT 1 cut(s) 169
TaiI ACGT 1 cut(s) 445
TaqI TCGA 2 cut(s) 101, 222
TasI AATT 3 cut(s) 18, 182, 414
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 1 cut(s) 125
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 1 cut(s) 409
TseI GCWGC 1 cut(s) 23
TspDTI ATGAA 3 cut(s) 205, 281, 400
TspRI CASTG 1 cut(s) 409
XcmI CCANNNNNNNNNTGG 1 cut(s) 317
XhoI CTCGAG 1 cut(s) 100
XspI CTAG 3 cut(s) 117, 129, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.