pycom16g14140

RING-like zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001780
Physical Location & Seq
Forward (+)
10186168 .. 10188400
2233 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g14140.4

Sequence Viewer

Length: 1011 bp
ATGGATTCTCCAACATCGTCCGTTTTGTCGTCGCCCTCAGCCGTGAAATGCTCCATCTGCATGGAGGGCGTCTCCGACGACTGTGGCCGCACTGTCGTCAAGCTTCAGTGCTCTGACTTGTTTCACCTCGATTGCATTGGTTCTGCATTCAACGTAAAGGGTATCATGGAATGCCCTATCTGCGGCGAGATTGAGGATGGCGTATGGAGGTACTTTATATCTGGGAGCCCTGACCGTGAGCTCAACGTATCTGAGGACGAGATGGATTACGAGAACGGCTCAGAACCACATTTTGAAGGGAATTTGCTTGCCCCCTATTCGGTCTTTCCTGGCTATCCTTATACCAACAATCAATGGAATGGCCCTCCCGTACCCAGCGAGGTATTGCCTTCGAATCCTTCCCGGATTGACTGGAGAACCGAACCTTTACTTTGTGATAGCTATACCAATTTGTTTCCAACGGGCAGCTATACTGGTGGTGCTAATTGGTCTTCAGTTCCTTCCGCAACCTTGAGTTCCGGGGTTGATTTTACTGGCATGCCAAATGCACCGTTTGTTCCTCAAATTGCTGACTGCGGCATGCGTGTTCAAACCAGTTATGAATCTCCATTTGGTCTTGCTTATCAAGGAAGCAATGATCCACTTCCTCCCAACTTCCAAATGATGCAGCAGTCGCCAGCCAATTTCCCTTCACAAGCAGCGCAAGCTTCCATGGGAAGATCAAATGACGCGCCTTCACTTGGAAGTTGGGCAGGGCAGCCTGGCTTGATGGAAGTTGGAGTGTCCGTGCAACCAAGCGTGATGGACTGGCTTCGGTCAGGGCAGCCAGCACCTTCACTTGAACAGGCTGCAGACTCCAATTCATTTCTCGTCAACATGAACTCTGACCTGTCGGAATCAGCAACCAACGGCCATCGCCTTGTCCAGGAAGGAAACGATGTCCAACCTTTCCATCTTTTTCCAGAAGACGCGGGTCCTCTGACTTATTTTTTCCCTGAAGCGAATCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

337

Amino Acids

36.26

Weight (kDa)

4.09

Isoelectric Point (pI)

53.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 731, 971
AciI CCGC 5 cut(s) 88, 183, 504, 576, 971
AclWI GGATC 1 cut(s) 632
AcoI YGGCCR 2 cut(s) 85, 910
AcsI RAATTY 1 cut(s) 301
AcuI CTGAAG 2 cut(s) 89, 477
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 2 cut(s) 212, 372
AfiI CCNNNNNNNGG 4 cut(s) 182, 319, 740, 925
AgsI TTSAA 4 cut(s) 151, 296, 590, 842
AjnI CCWGG 3 cut(s) 328, 760, 924
AjuI GAANNNNNNNTTGG 2 cut(s) 594, 626
AluBI AGCT 5 cut(s) 103, 241, 441, 468, 707
AluI AGCT 5 cut(s) 103, 241, 441, 468, 707
Alw21I GWGCWC 2 cut(s) 113, 243
Alw26I GTCTC 1 cut(s) 76
AlwI GGATC 1 cut(s) 632
AoxI GGCC 3 cut(s) 85, 361, 910
ApeKI GCWGC 6 cut(s) 465, 667, 698, 757, 823, 848
ApoI RAATTY 1 cut(s) 301
AspLEI GCGC 2 cut(s) 703, 733
AspS9I GGNCC 2 cut(s) 362, 974
AsuC2I CCSGG 2 cut(s) 403, 520
AsuHPI GGTGA 1 cut(s) 116
AsuII TTCGAA 1 cut(s) 392
AvaII GGWCC 1 cut(s) 974
BanII GRGCYC 2 cut(s) 230, 243
BbsI GAAGAC 2 cut(s) 483, 972
Bbv12I GWGCWC 2 cut(s) 113, 243
BbvCI CCTCAGC 1 cut(s) 37
BbvI GCAGC 6 cut(s) 477, 679, 710, 769, 835, 835
BccI CCATC 7 cut(s) 62, 191, 256, 763, 796, 921, 960
BceAI ACGGC 3 cut(s) 26, 292, 925
BciT130I CCWGG 3 cut(s) 330, 762, 926
BcnI CCSGG 2 cut(s) 403, 520
BcoDI GTCTC 1 cut(s) 76
BfmI CTRYAG 1 cut(s) 849
BisI GCNGC 9 cut(s) 88, 184, 466, 577, 668, 699, 758, 824, 849
BlsI GCNGC 9 cut(s) 89, 185, 467, 578, 669, 700, 759, 825, 850
Bme1390I CCNGG 5 cut(s) 330, 403, 520, 762, 926
Bme18I GGWCC 1 cut(s) 974
BmgT120I GGNCC 2 cut(s) 362, 974
BmiI GGNNCC 2 cut(s) 227, 975
BmrFI CCNGG 5 cut(s) 330, 403, 520, 762, 926
BmsI GCATC 1 cut(s) 654
BoxI GACNNNNGTC 1 cut(s) 972
BpiI GAAGAC 2 cut(s) 483, 972
BplI GAGNNNNNCTC 4 cut(s) 56, 88, 263, 295
BpmI CTGGAG 1 cut(s) 433
Bpu10I CCTNAGC 1 cut(s) 37
Bpu14I TTCGAA 1 cut(s) 392
BpuEI CTTGAG 1 cut(s) 532
BpuMI CCSGG 2 cut(s) 403, 520
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 2 cut(s) 519, 711
BsaXI ACNNNNNCTCC 2 cut(s) 771, 801
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 319, 740, 925
Bse1I ACTGG 5 cut(s) 416, 478, 538, 594, 812
Bse3DI GCAATG 1 cut(s) 640
BseBI CCWGG 3 cut(s) 330, 762, 926
BseDI CCNNGG 2 cut(s) 519, 711
BseGI GGATG 1 cut(s) 202
BseLI CCNNNNNNNGG 4 cut(s) 182, 319, 740, 925
BseMI GCAATG 1 cut(s) 640
BseMII CTCAG 3 cut(s) 51, 243, 294
BseNI ACTGG 5 cut(s) 416, 478, 538, 594, 812
BseXI GCAGC 6 cut(s) 477, 679, 710, 769, 835, 835
BseYI CCCAGC 1 cut(s) 374
Bsh1236I CGCG 2 cut(s) 731, 971
BshFI GGCC 3 cut(s) 87, 363, 912
BsiHKAI GWGCWC 2 cut(s) 113, 243
BsiSI CCGG 2 cut(s) 403, 519
BslI CCNNNNNNNGG 4 cut(s) 182, 319, 740, 925
BsmAI GTCTC 1 cut(s) 76
BsmBI CGTCTC 1 cut(s) 76
BsmI GAATGC 2 cut(s) 146, 176
BsnI GGCC 3 cut(s) 87, 363, 912
Bsp119I TTCGAA 1 cut(s) 392
Bsp1286I GDGCHC 3 cut(s) 113, 230, 243
Bsp143I GATC 2 cut(s) 637, 719
Bsp19I CCATGG 1 cut(s) 711
BspACI CCGC 5 cut(s) 88, 183, 504, 576, 971
BspANI GGCC 3 cut(s) 87, 363, 912
BspCNI CTCAG 3 cut(s) 50, 244, 293
BspFNI CGCG 2 cut(s) 731, 971
BspLI GGNNCC 2 cut(s) 227, 975
BspMAI CTGCAG 1 cut(s) 853
BspPI GGATC 1 cut(s) 632
BspT104I TTCGAA 1 cut(s) 392
BsrDI GCAATG 1 cut(s) 640
BsrI ACTGG 5 cut(s) 416, 478, 538, 594, 812
BssECI CCNNGG 2 cut(s) 519, 711
BssMI GATC 2 cut(s) 637, 719
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 711
Bst2UI CCWGG 3 cut(s) 330, 762, 926
Bst4CI ACNGT 4 cut(s) 83, 94, 236, 552
BstACI GRCGYC 1 cut(s) 69
BstBI TTCGAA 1 cut(s) 392
BstC8I GCNNGC 6 cut(s) 309, 539, 581, 678, 705, 828
BstDEI CTNAG 3 cut(s) 37, 252, 280
BstDSI CCRYGG 1 cut(s) 711
BstENI CCTNNNNNAGG 1 cut(s) 923
BstF5I GGATG 1 cut(s) 202
BstFNI CGCG 2 cut(s) 731, 971
BstHHI GCGC 2 cut(s) 703, 733
BstKTI GATC 2 cut(s) 640, 722
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 2 cut(s) 637, 719
BstMWI GCNNNNNNNGC 5 cut(s) 57, 66, 180, 673, 704
BstNI CCWGG 3 cut(s) 330, 762, 926
BstNSI RCATGY 2 cut(s) 541, 583
BstPAI GACNNNNGTC 1 cut(s) 972
BstSCI CCNGG 5 cut(s) 328, 401, 518, 760, 924
BstSFI CTRYAG 1 cut(s) 849
BstUI CGCG 2 cut(s) 731, 971
BstV1I GCAGC 6 cut(s) 477, 679, 710, 769, 835, 835
BstV2I GAAGAC 2 cut(s) 483, 972
BstXI CCANNNNNNTGG 1 cut(s) 61
BsuRI GGCC 3 cut(s) 87, 363, 912
BtgI CCRYGG 1 cut(s) 711
BtgZI GCGATG 1 cut(s) 899
BtsCI GGATG 1 cut(s) 202
BtsIMutI CAGTG 2 cut(s) 90, 113
Cac8I GCNNGC 6 cut(s) 309, 539, 581, 678, 705, 828
CfoI GCGC 2 cut(s) 703, 733
Cfr13I GGNCC 2 cut(s) 362, 974
CseI GACGC 3 cut(s) 58, 737, 977
Csp6I GTAC 2 cut(s) 211, 371
CviAII CATG 6 cut(s) 61, 166, 538, 580, 712, 877
CviQI GTAC 2 cut(s) 211, 371
DdeI CTNAG 3 cut(s) 37, 252, 280
DpnI GATC 2 cut(s) 639, 721
DpnII GATC 2 cut(s) 637, 719
EaeI YGGCCR 2 cut(s) 85, 910
Ecl136II GAGCTC 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 711
Eco24I GRGCYC 2 cut(s) 230, 243
Eco47I GGWCC 1 cut(s) 974
Eco53kI GAGCTC 1 cut(s) 241
Eco57I CTGAAG 2 cut(s) 89, 477
EcoICRI GAGCTC 1 cut(s) 241
EcoNI CCTNNNNNAGG 1 cut(s) 923
EcoO109I RGGNCCY 1 cut(s) 974
EcoRII CCWGG 3 cut(s) 328, 760, 924
EcoT14I CCWWGG 1 cut(s) 711
EcoT38I GRGCYC 2 cut(s) 230, 243
ErhI CCWWGG 1 cut(s) 711
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 6 cut(s) 64, 169, 541, 583, 715, 880
FatI CATG 6 cut(s) 60, 165, 537, 579, 711, 876
FauI CCCGC 1 cut(s) 964
Fnu4HI GCNGC 9 cut(s) 88, 184, 466, 577, 668, 699, 758, 824, 849
FokI GGATG 1 cut(s) 209
FriOI GRGCYC 2 cut(s) 230, 243
Fsp4HI GCNGC 9 cut(s) 88, 184, 466, 577, 668, 699, 758, 824, 849
GlaI GCGC 2 cut(s) 702, 732
GluI GCNGC 9 cut(s) 88, 184, 466, 577, 668, 699, 758, 824, 849
GsaI CCCAGC 1 cut(s) 378
GsuI CTGGAG 1 cut(s) 433
HaeIII GGCC 3 cut(s) 87, 363, 912
HapII CCGG 2 cut(s) 403, 519
HgaI GACGC 3 cut(s) 58, 737, 977
HhaI GCGC 2 cut(s) 703, 733
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 6 cut(s) 64, 169, 541, 583, 715, 880
Hin6I GCGC 2 cut(s) 701, 731
HinP1I GCGC 2 cut(s) 701, 731
HincII GTYRAC 1 cut(s) 874
HindII GTYRAC 1 cut(s) 874
HindIII AAGCTT 2 cut(s) 101, 705
HinfI GANTC 6 cut(s) 5, 394, 602, 854, 896, 1003
HpaII CCGG 2 cut(s) 403, 519
HphI GGTGA 1 cut(s) 116
Hpy166II GTNNAC 1 cut(s) 874
Hpy188I TCNGA 7 cut(s) 76, 115, 253, 283, 886, 895, 981
Hpy188III TCNNGA 1 cut(s) 962
Hpy8I GTNNAC 1 cut(s) 874
Hpy99I CGWCG 2 cut(s) 34, 80
HpyAV CCTTC 8 cut(s) 290, 399, 408, 510, 699, 744, 843, 923
HpyCH4III ACNGT 4 cut(s) 83, 94, 236, 552
HpyCH4IV ACGT 2 cut(s) 153, 246
HpyCH4V TGCA 7 cut(s) 60, 135, 146, 548, 667, 790, 851
HpyF10VI GCNNNNNNNGC 5 cut(s) 57, 66, 180, 673, 704
HpyF3I CTNAG 3 cut(s) 37, 252, 280
HpySE526I ACGT 2 cut(s) 153, 246
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 6 cut(s) 64, 169, 541, 583, 715, 880
HspAI GCGC 2 cut(s) 701, 731
Kzo9I GATC 2 cut(s) 637, 719
LmnI GCTCC 2 cut(s) 56, 225
Lsp1109I GCAGC 6 cut(s) 477, 679, 710, 769, 835, 835
LweI GCATC 1 cut(s) 654
MaeII ACGT 2 cut(s) 153, 246
MalI GATC 2 cut(s) 639, 721
MboI GATC 2 cut(s) 637, 719
MboII GAAGA 3 cut(s) 483, 729, 977
MhlI GDGCHC 3 cut(s) 113, 230, 243
MluCI AATT 6 cut(s) 301, 448, 484, 564, 682, 859
MlyI GAGTC 1 cut(s) 848
MmeI TCCRAC 6 cut(s) 35, 99, 482, 757, 873, 967
MslI CAYNNNNRTG 1 cut(s) 59
MspI CCGG 2 cut(s) 403, 519
MspR9I CCNGG 5 cut(s) 330, 403, 520, 762, 926
Mva1269I GAATGC 2 cut(s) 146, 176
MvaI CCWGG 3 cut(s) 330, 762, 926
MvnI CGCG 2 cut(s) 731, 971
MwoI GCNNNNNNNGC 5 cut(s) 57, 66, 180, 673, 704
NciI CCSGG 2 cut(s) 403, 520
NcoI CCATGG 1 cut(s) 711
NdeII GATC 2 cut(s) 637, 719
NlaIII CATG 6 cut(s) 64, 169, 541, 583, 715, 880
NlaIV GGNNCC 2 cut(s) 227, 975
NspI RCATGY 2 cut(s) 541, 583
NspV TTCGAA 1 cut(s) 392
PaeI GCATGC 2 cut(s) 541, 583
PctI GAATGC 2 cut(s) 146, 176
PfeI GAWTC 5 cut(s) 5, 394, 602, 896, 1003
PfoI TCCNGGA 2 cut(s) 401, 924
PkrI GCNGC 9 cut(s) 89, 185, 467, 578, 669, 700, 759, 825, 850
PleI GAGTC 1 cut(s) 848
PpsI GAGTC 1 cut(s) 848
PpuMI RGGWCCY 1 cut(s) 974
PshAI GACNNNNGTC 1 cut(s) 972
Psp124BI GAGCTC 1 cut(s) 243
Psp5II RGGWCCY 1 cut(s) 974
Psp6I CCWGG 3 cut(s) 328, 760, 924
PspFI CCCAGC 1 cut(s) 374
PspGI CCWGG 3 cut(s) 328, 760, 924
PspN4I GGNNCC 2 cut(s) 227, 975
PspPI GGNCC 2 cut(s) 362, 974
PspPPI RGGWCCY 1 cut(s) 974
PstI CTGCAG 1 cut(s) 853
RsaI GTAC 2 cut(s) 212, 372
RsaNI GTAC 2 cut(s) 211, 371
RseI CAYNNNNRTG 1 cut(s) 59
SacI GAGCTC 1 cut(s) 243
SatI GCNGC 9 cut(s) 88, 184, 466, 577, 668, 699, 758, 824, 849
Sau3AI GATC 2 cut(s) 637, 719
Sau96I GGNCC 2 cut(s) 362, 974
SchI GAGTC 1 cut(s) 848
ScrFI CCNGG 5 cut(s) 330, 403, 520, 762, 926
SduI GDGCHC 3 cut(s) 113, 230, 243
SfaNI GCATC 1 cut(s) 654
SfcI CTRYAG 1 cut(s) 849
SfuI TTCGAA 1 cut(s) 392
SinI GGWCC 1 cut(s) 974
SmiMI CAYNNNNRTG 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 511
SmoI CTYRAG 1 cut(s) 511
SphI GCATGC 2 cut(s) 541, 583
Sse9I AATT 6 cut(s) 301, 448, 484, 564, 682, 859
SsiI CCGC 5 cut(s) 88, 183, 504, 576, 971
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 5 cut(s) 328, 401, 518, 760, 924
StyI CCWWGG 1 cut(s) 711
TaaI ACNGT 4 cut(s) 83, 94, 236, 552
TaiI ACGT 2 cut(s) 156, 249
TaqI TCGA 2 cut(s) 129, 392
TaqII GACCGA 2 cut(s) 310, 804
TasI AATT 6 cut(s) 301, 448, 484, 564, 682, 859
TauI GCSGC 3 cut(s) 90, 186, 579
TfiI GAWTC 5 cut(s) 5, 394, 602, 896, 1003
TscAI CASTG 2 cut(s) 97, 113
TseI GCWGC 6 cut(s) 465, 667, 698, 757, 823, 848
TspDTI ATGAA 3 cut(s) 615, 852, 893
TspGWI ACGGA 2 cut(s) 10, 775
TspRI CASTG 2 cut(s) 97, 113
VpaK11BI GGWCC 1 cut(s) 974
XagI CCTNNNNNAGG 1 cut(s) 923
XapI RAATTY 1 cut(s) 301
XceI RCATGY 2 cut(s) 541, 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.