RchiOBHm_Chr7g0217821
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001780
Physical Location & Seq
Forward (+)
35784300 .. 35788218
3919 bp
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UTR
Exon/CDS
Intron
PRQ19495

Sequence Viewer

Length: 552 bp
ATGTGCTTGCATCTTTCTGCATATTATAATTTTGGGATTAATTGTGGAGGACCTGAGATTACGTCTTCCTCCAATGGGAGAGTGTATGAGAAGGAAAATGAGCCCCTTGGACCAGCTTCATATTTTGTGACTGGCACAAGTAGATGGGGAGTTAGCAATGTCGGATATTTCGCTGGCAGTACCAATCCTCAGTATACAAGTTTCTCCTTATCTCAATTCACAAATGCTTTTGATTCTGTGCTATTCCAGACTGCAAGGCTCTCTGCTTCATCAATTAGATACTTTGGCTTGGGGCTTGAGAATGGTAACTACACCGTGAACCTCAAGTTTGCAGAACATGTCATTCTGGCTGCTACTACCGGGAAATTTCTTGGGAGACGTCTTTTTGATATTTATATCCAGATAGATATATGGAGTAGATCCAAAAGTAATCACCACTCAGTTACCCAAGCTCCAGAGTGGGAGAAAAAAGGGAAAGCAGCATATACCCAGCAACATATTGATGAAGTCAGAAACGAGTGGGCAAAATTTGTTGTTAAAACATATATGTAA

Protein Analysis

183

Amino Acids

20.6

Weight (kDa)

8.82

Isoelectric Point (pI)

26.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 11 - 135 1.1e-20 Malectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 27
AatII GACGTC 1 cut(s) 382
AccI GTMKAC 1 cut(s) 194
AclWI GGATC 1 cut(s) 414
AcsI RAATTY 2 cut(s) 365, 527
AcyI GRCGYC 1 cut(s) 379
AfaI GTAC 1 cut(s) 181
AfiI CCNNNNNNNGG 1 cut(s) 75
AflIII ACRYGT 1 cut(s) 337
AluBI AGCT 2 cut(s) 116, 452
AluI AGCT 2 cut(s) 116, 452
Alw26I GTCTC 1 cut(s) 370
AlwI GGATC 1 cut(s) 414
ApeKI GCWGC 2 cut(s) 350, 479
ApoI RAATTY 2 cut(s) 365, 527
AseI ATTAAT 1 cut(s) 39
AspS9I GGNCC 2 cut(s) 50, 110
AsuC2I CCSGG 1 cut(s) 361
AsuHPI GGTGA 1 cut(s) 425
AvaII GGWCC 2 cut(s) 50, 110
BanII GRGCYC 1 cut(s) 105
BbsI GAAGAC 1 cut(s) 57
BbvI GCAGC 2 cut(s) 337, 491
BccI CCATC 1 cut(s) 138
BcnI CCSGG 1 cut(s) 361
BcoDI GTCTC 1 cut(s) 370
BisI GCNGC 2 cut(s) 351, 480
BlsI GCNGC 2 cut(s) 352, 481
Bme1390I CCNGG 1 cut(s) 361
Bme18I GGWCC 2 cut(s) 50, 110
BmgT120I GGNCC 2 cut(s) 50, 110
BmrFI CCNGG 1 cut(s) 361
BmsI GCATC 1 cut(s) 19
BpiI GAAGAC 1 cut(s) 57
BpmI CTGGAG 1 cut(s) 438
BpuEI CTTGAG 2 cut(s) 308, 317
BpuMI CCSGG 1 cut(s) 361
BsaHI GRCGYC 1 cut(s) 379
BsaJI CCNNGG 1 cut(s) 106
BsaXI ACNNNNNCTCC 2 cut(s) 436, 466
Bsc4I CCNNNNNNNGG 1 cut(s) 75
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 1 cut(s) 163
BseDI CCNNGG 1 cut(s) 106
BseLI CCNNNNNNNGG 1 cut(s) 75
BseMI GCAATG 1 cut(s) 163
BseMII CTCAG 3 cut(s) 45, 203, 453
BseNI ACTGG 1 cut(s) 136
BseXI GCAGC 2 cut(s) 337, 491
BseYI CCCAGC 1 cut(s) 489
BsiSI CCGG 1 cut(s) 360
BslI CCNNNNNNNGG 1 cut(s) 75
BsmAI GTCTC 1 cut(s) 370
BsmBI CGTCTC 1 cut(s) 370
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 1 cut(s) 419
BspCNI CTCAG 3 cut(s) 46, 202, 452
BspPI GGATC 1 cut(s) 414
BsrDI GCAATG 1 cut(s) 163
BsrI ACTGG 1 cut(s) 136
BssECI CCNNGG 1 cut(s) 106
BssMI GATC 1 cut(s) 419
BssNAI GTATAC 1 cut(s) 195
BssNI GRCGYC 1 cut(s) 379
BssT1I CCWWGG 1 cut(s) 106
Bst1107I GTATAC 1 cut(s) 195
Bst4CI ACNGT 1 cut(s) 316
BstACI GRCGYC 1 cut(s) 379
BstC8I GCNNGC 2 cut(s) 8, 175
BstDEI CTNAG 3 cut(s) 54, 189, 439
BstKTI GATC 1 cut(s) 422
BstMAI GTCTC 1 cut(s) 370
BstMBI GATC 1 cut(s) 419
BstNSI RCATGY 1 cut(s) 341
BstSCI CCNGG 1 cut(s) 359
BstV1I GCAGC 2 cut(s) 337, 491
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 1 cut(s) 419
BstYI RGATCY 1 cut(s) 419
BstZ17I GTATAC 1 cut(s) 195
Cac8I GCNNGC 2 cut(s) 8, 175
Cfr13I GGNCC 2 cut(s) 50, 110
Csp6I GTAC 1 cut(s) 180
CviAII CATG 1 cut(s) 338
CviJI RGCY 7 cut(s) 103, 116, 259, 288, 295, 350, 452
CviKI_1 RGCY 7 cut(s) 103, 116, 259, 288, 295, 350, 452
CviQI GTAC 1 cut(s) 180
DdeI CTNAG 3 cut(s) 54, 189, 439
DpnI GATC 1 cut(s) 421
DpnII GATC 1 cut(s) 419
Eco130I CCWWGG 1 cut(s) 106
Eco24I GRGCYC 1 cut(s) 105
Eco47I GGWCC 2 cut(s) 50, 110
EcoO109I RGGNCCY 1 cut(s) 50
EcoT14I CCWWGG 1 cut(s) 106
EcoT38I GRGCYC 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 106
Esp3I CGTCTC 1 cut(s) 370
FaeI CATG 1 cut(s) 341
FatI CATG 1 cut(s) 337
FblI GTMKAC 1 cut(s) 194
Fnu4HI GCNGC 2 cut(s) 351, 480
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 2 cut(s) 351, 480
GluI GCNGC 2 cut(s) 351, 480
GsaI CCCAGC 1 cut(s) 493
GsuI CTGGAG 1 cut(s) 438
HapII CCGG 1 cut(s) 360
Hin1I GRCGYC 1 cut(s) 379
Hin1II CATG 1 cut(s) 341
HinfI GANTC 1 cut(s) 233
HpaII CCGG 1 cut(s) 360
HphI GGTGA 1 cut(s) 425
Hpy166II GTNNAC 2 cut(s) 195, 319
Hpy188I TCNGA 2 cut(s) 164, 512
Hpy188III TCNNGA 3 cut(s) 247, 400, 455
Hpy8I GTNNAC 2 cut(s) 195, 319
HpyAV CCTTC 1 cut(s) 85
HpyCH4III ACNGT 1 cut(s) 316
HpyCH4IV ACGT 2 cut(s) 62, 379
HpyCH4V TGCA 4 cut(s) 10, 20, 254, 332
HpyF3I CTNAG 3 cut(s) 54, 189, 439
HpySE526I ACGT 2 cut(s) 62, 379
Hsp92I GRCGYC 1 cut(s) 379
Hsp92II CATG 1 cut(s) 341
Kzo9I GATC 1 cut(s) 419
LmnI GCTCC 1 cut(s) 457
Lsp1109I GCAGC 2 cut(s) 337, 491
LweI GCATC 1 cut(s) 19
MaeII ACGT 2 cut(s) 62, 379
MaeIII GTNAC 3 cut(s) 127, 305, 442
MalI GATC 1 cut(s) 421
MboI GATC 1 cut(s) 419
MboII GAAGA 1 cut(s) 57
MflI RGATCY 1 cut(s) 419
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 6 cut(s) 28, 40, 215, 273, 365, 527
MmeI TCCRAC 1 cut(s) 142
MnlI CCTC 4 cut(s) 41, 79, 198, 332
MseI TTAA 2 cut(s) 39, 537
MslI CAYNNNNRTG 1 cut(s) 501
MspI CCGG 1 cut(s) 360
MspR9I CCNGG 1 cut(s) 361
NciI CCSGG 1 cut(s) 361
NdeII GATC 1 cut(s) 419
NlaIII CATG 1 cut(s) 341
NmuCI GTSAC 1 cut(s) 127
NspI RCATGY 1 cut(s) 341
PciI ACATGT 1 cut(s) 337
PfeI GAWTC 1 cut(s) 233
PkrI GCNGC 2 cut(s) 352, 481
PpuMI RGGWCCY 1 cut(s) 50
PscI ACATGT 1 cut(s) 337
PshBI ATTAAT 1 cut(s) 39
PsiI TTATAA 1 cut(s) 27
Psp5II RGGWCCY 1 cut(s) 50
PspFI CCCAGC 1 cut(s) 489
PspPI GGNCC 2 cut(s) 50, 110
PspPPI RGGWCCY 1 cut(s) 50
PsuI RGATCY 1 cut(s) 419
RsaI GTAC 1 cut(s) 181
RsaNI GTAC 1 cut(s) 180
RseI CAYNNNNRTG 1 cut(s) 501
SaqAI TTAA 2 cut(s) 39, 537
SatI GCNGC 2 cut(s) 351, 480
Sau3AI GATC 1 cut(s) 419
Sau96I GGNCC 2 cut(s) 50, 110
ScrFI CCNGG 1 cut(s) 361
SduI GDGCHC 1 cut(s) 105
SetI ASST 6 cut(s) 55, 65, 118, 324, 382, 454
SfaNI GCATC 1 cut(s) 19
SinI GGWCC 2 cut(s) 50, 110
SmiMI CAYNNNNRTG 1 cut(s) 501
SmlI CTYRAG 2 cut(s) 296, 323
SmoI CTYRAG 2 cut(s) 296, 323
Sse9I AATT 6 cut(s) 28, 40, 215, 273, 365, 527
StyD4I CCNGG 1 cut(s) 359
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 1 cut(s) 316
TaiI ACGT 2 cut(s) 65, 382
TasI AATT 6 cut(s) 28, 40, 215, 273, 365, 527
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 2 cut(s) 39, 537
Tru9I TTAA 2 cut(s) 39, 537
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 2 cut(s) 350, 479
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 3 cut(s) 108, 258, 519
VpaK11BI GGWCC 2 cut(s) 50, 110
VspI ATTAAT 1 cut(s) 39
XapI RAATTY 2 cut(s) 365, 527
XceI RCATGY 1 cut(s) 341
XmiI GTMKAC 1 cut(s) 194
ZraI GACGTC 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.