Rh4CG255600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001780
Physical Location & Seq
Forward (+)
50728628 .. 50742474
13847 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG255600.1

Sequence Viewer

Length: 570 bp
ATGGAAATTTCCCGCCTGTGCAATCAAAATTTCAGTTTTGGCGCTGAGGGGCGTGACACTGGGGATGGCGACGCGGTGAGAGTAGAAAAGGCCGGGCAACACTACGCAGACTCTGGAGGCGAAGAGGGTGTCGGTGGAAAGGCGAAGAGCACTGGATGGGAGGAGCGTGGGGAGGATGGAGCTAGAGGAGATAGATTGGAGTTGGAGAGTGAGAGAGAGAGGTTTTATAACATGACTGCCAGTGGCAGGCAGTGGGATCCATTATTGTTGGACTATAGATTTGCTGTAGACCCCAATGGTGGTATTCCTAATAATCATCAACCGAATGGTCCTTCTGAAGTATCCGAACCAATAGAGAGTCCTCTACGTTATGGAATTTGTGATGATTGTATCAACACACATCTGTCAAGTGGCCATGCTGGTCATAGTAATGGGCTTTCAGTTCCAGATGGAAACCTGGGAATTCCTAGTGATGATGTTAATGCCAATATTCCAAGTGATTTCATAATTCCTCCTGCTGATTTCATAATTCCTCCTGCTGGTTTTCATAATTCCTCCTGCTGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

189

Amino Acids

20.37

Weight (kDa)

4.51

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 228
AccI GTMKAC 1 cut(s) 288
AccII CGCG 1 cut(s) 74
AciI CCGC 2 cut(s) 13, 74
AclWI GGATC 2 cut(s) 251, 264
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 4 cut(s) 6, 28, 375, 462
AcuI CTGAAG 1 cut(s) 357
AfiI CCNNNNNNNGG 4 cut(s) 246, 299, 539, 561
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 1 cut(s) 182
AluI AGCT 1 cut(s) 182
Alw21I GWGCWC 1 cut(s) 152
AlwI GGATC 2 cut(s) 251, 264
AlwNI CAGNNNCTG 1 cut(s) 113
AoxI GGCC 2 cut(s) 90, 412
ApoI RAATTY 4 cut(s) 6, 28, 375, 462
ArsI GACNNNNNNTTYG 4 cut(s) 114, 146, 263, 295
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 1 cut(s) 329
AsuC2I CCSGG 1 cut(s) 94
AsuHPI GGTGA 1 cut(s) 88
AvaII GGWCC 1 cut(s) 329
BalI TGGCCA 1 cut(s) 414
BamHI GGATCC 1 cut(s) 256
Bbv12I GWGCWC 1 cut(s) 152
BbvCI CCTCAGC 1 cut(s) 45
BccI CCATC 4 cut(s) 59, 150, 170, 443
BciT130I CCWGG 1 cut(s) 458
BciVI GTATCC 1 cut(s) 352
BcnI CCSGG 1 cut(s) 94
BfaI CTAG 2 cut(s) 183, 468
BfmI CTRYAG 2 cut(s) 274, 285
BfoI RGCGCY 1 cut(s) 45
BfuI GTATCC 1 cut(s) 352
Bme1390I CCNGG 2 cut(s) 94, 458
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 1 cut(s) 258
BmrFI CCNGG 2 cut(s) 94, 458
BmrI ACTGGG 1 cut(s) 69
BmuI ACTGGG 1 cut(s) 69
BpmI CTGGAG 1 cut(s) 135
Bpu10I CCTNAGC 1 cut(s) 45
BpuMI CCSGG 1 cut(s) 94
BsaJI CCNNGG 1 cut(s) 457
Bsc4I CCNNNNNNNGG 4 cut(s) 246, 299, 539, 561
Bse1I ACTGG 3 cut(s) 64, 157, 240
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 1 cut(s) 457
BseGI GGATG 3 cut(s) 70, 161, 181
BseLI CCNNNNNNNGG 4 cut(s) 246, 299, 539, 561
BseMII CTCAG 1 cut(s) 36
BseNI ACTGG 3 cut(s) 64, 157, 240
BseRI GAGGAG 2 cut(s) 176, 201
Bsh1236I CGCG 1 cut(s) 74
BshFI GGCC 2 cut(s) 92, 414
BsiHKAI GWGCWC 1 cut(s) 152
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 4 cut(s) 246, 299, 539, 561
BsnI GGCC 2 cut(s) 92, 414
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 1 cut(s) 256
BspACI CCGC 2 cut(s) 13, 74
BspANI GGCC 2 cut(s) 92, 414
BspCNI CTCAG 1 cut(s) 37
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 1 cut(s) 258
BspPI GGATC 2 cut(s) 251, 264
BspQI GCTCTTC 1 cut(s) 140
BsrI ACTGG 3 cut(s) 64, 157, 240
BssECI CCNNGG 1 cut(s) 457
BssMI GATC 1 cut(s) 256
Bst2UI CCWGG 1 cut(s) 458
Bst6I CTCTTC 2 cut(s) 117, 140
BstC8I GCNNGC 1 cut(s) 248
BstDEI CTNAG 1 cut(s) 45
BstF5I GGATG 3 cut(s) 70, 161, 181
BstFNI CGCG 1 cut(s) 74
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 1 cut(s) 259
BstMBI GATC 1 cut(s) 256
BstNI CCWGG 1 cut(s) 458
BstSCI CCNGG 2 cut(s) 92, 456
BstSFI CTRYAG 2 cut(s) 274, 285
BstUI CGCG 1 cut(s) 74
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
BsuI GTATCC 1 cut(s) 352
BsuRI GGCC 2 cut(s) 92, 414
BtsCI GGATG 3 cut(s) 70, 161, 181
BtsI GCAGTG 1 cut(s) 257
BtsIMutI CAGTG 4 cut(s) 57, 150, 247, 257
Cac8I GCNNGC 1 cut(s) 248
CaiI CAGNNNCTG 1 cut(s) 113
CfoI GCGC 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 329
CseI GACGC 1 cut(s) 80
CviAII CATG 2 cut(s) 232, 416
CviJI RGCY 4 cut(s) 92, 182, 414, 436
CviKI_1 RGCY 4 cut(s) 92, 182, 414, 436
DdeI CTNAG 1 cut(s) 45
DpnI GATC 1 cut(s) 258
DpnII GATC 1 cut(s) 256
EaeI YGGCCR 1 cut(s) 412
Eam1104I CTCTTC 2 cut(s) 117, 140
EarI CTCTTC 2 cut(s) 117, 140
Eco47I GGWCC 1 cut(s) 329
Eco57I CTGAAG 1 cut(s) 357
EcoRI GAATTC 1 cut(s) 462
EcoRII CCWGG 1 cut(s) 456
FaeI CATG 2 cut(s) 235, 419
FaiI YATR 9 cut(s) 228, 233, 276, 372, 417, 426, 506, 527, 549
FatI CATG 2 cut(s) 231, 415
FauI CCCGC 1 cut(s) 20
FblI GTMKAC 1 cut(s) 288
FokI GGATG 3 cut(s) 77, 168, 188
FspBI CTAG 2 cut(s) 183, 468
GlaI GCGC 1 cut(s) 43
GsuI CTGGAG 1 cut(s) 135
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 2 cut(s) 92, 414
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 80
HhaI GCGC 1 cut(s) 44
Hin1II CATG 2 cut(s) 235, 419
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HinfI GANTC 2 cut(s) 110, 358
HpaII CCGG 1 cut(s) 93
HphI GGTGA 1 cut(s) 88
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 2 cut(s) 337, 346
Hpy188III TCNNGA 2 cut(s) 114, 446
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 1 cut(s) 342
HpyCH4IV ACGT 1 cut(s) 367
HpyCH4V TGCA 1 cut(s) 21
HpyF3I CTNAG 1 cut(s) 45
HpySE526I ACGT 1 cut(s) 367
Hsp92II CATG 2 cut(s) 235, 419
HspAI GCGC 1 cut(s) 42
Kzo9I GATC 1 cut(s) 256
LguI GCTCTTC 1 cut(s) 140
LmnI GCTCC 2 cut(s) 163, 179
MaeI CTAG 2 cut(s) 183, 468
MaeII ACGT 1 cut(s) 367
MaeIII GTNAC 1 cut(s) 53
MalI GATC 1 cut(s) 258
MboI GATC 1 cut(s) 256
MboII GAAGA 2 cut(s) 134, 157
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 1 cut(s) 152
MlsI TGGCCA 1 cut(s) 414
MluCI AATT 7 cut(s) 6, 28, 375, 462, 507, 528, 550
MluNI TGGCCA 1 cut(s) 414
MlyI GAGTC 2 cut(s) 104, 367
MmeI TCCRAC 2 cut(s) 183, 249
Mox20I TGGCCA 1 cut(s) 414
MscI TGGCCA 1 cut(s) 414
MseI TTAA 1 cut(s) 480
MslI CAYNNNNRTG 1 cut(s) 429
Msp20I TGGCCA 1 cut(s) 414
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 2 cut(s) 94, 458
MvaI CCWGG 1 cut(s) 458
MvnI CGCG 1 cut(s) 74
NciI CCSGG 1 cut(s) 94
NdeII GATC 1 cut(s) 256
NlaIII CATG 2 cut(s) 235, 419
NlaIV GGNNCC 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 53
PciSI GCTCTTC 1 cut(s) 140
PleI GAGTC 2 cut(s) 104, 366
PpsI GAGTC 2 cut(s) 104, 366
PsiI TTATAA 1 cut(s) 228
Psp6I CCWGG 1 cut(s) 456
PspGI CCWGG 1 cut(s) 456
PspN4I GGNNCC 1 cut(s) 258
PspPI GGNCC 1 cut(s) 329
PstNI CAGNNNCTG 1 cut(s) 113
PsuI RGATCY 1 cut(s) 256
RseI CAYNNNNRTG 1 cut(s) 429
SapI GCTCTTC 1 cut(s) 140
SaqAI TTAA 1 cut(s) 480
Sau3AI GATC 1 cut(s) 256
Sau96I GGNCC 1 cut(s) 329
SchI GAGTC 2 cut(s) 104, 367
ScrFI CCNGG 2 cut(s) 94, 458
SduI GDGCHC 1 cut(s) 152
SetI ASST 4 cut(s) 184, 224, 370, 459
SfcI CTRYAG 2 cut(s) 274, 285
SinI GGWCC 1 cut(s) 329
SmiMI CAYNNNNRTG 1 cut(s) 429
Sse9I AATT 7 cut(s) 6, 28, 375, 462, 507, 528, 550
SsiI CCGC 2 cut(s) 13, 74
SspI AATATT 1 cut(s) 490
SspMI CTAG 2 cut(s) 183, 468
StyD4I CCNGG 2 cut(s) 92, 456
TaiI ACGT 1 cut(s) 370
TasI AATT 7 cut(s) 6, 28, 375, 462, 507, 528, 550
Tru1I TTAA 1 cut(s) 480
Tru9I TTAA 1 cut(s) 480
TscAI CASTG 4 cut(s) 64, 157, 247, 257
TseFI GTSAC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 53
TspDTI ATGAA 3 cut(s) 493, 514, 536
TspRI CASTG 4 cut(s) 64, 157, 247, 257
VpaK11BI GGWCC 1 cut(s) 329
XapI RAATTY 4 cut(s) 6, 28, 375, 462
XmiI GTMKAC 1 cut(s) 288
XspI CTAG 2 cut(s) 183, 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.