Rh2AG249700
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001780
Physical Location & Seq
Forward (+)
28119756 .. 28246945
127190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG249700.1

Sequence Viewer

Length: 705 bp
ATGAGAAGCGACATGAGTATCAACAACTCAATGATTAGCATACATTATAATTTTGGGATTAATTGTGGAGGACCTGAGTTTACGTCTTCCTCCGATGGGATTGTGTATGAGAAGGAAAATGAGCCCCTTGGACCAGCTTCATATTTTGTGACTGGCACAAGCAGATGGGGAGTTAGCAATGTCGGACATTTCGCTGGCAGTACCAATCCTCAGTATACAAGCTTCTCCTTATCTCAATTCACAAATACTTTTGATTATGTGCTATTCCAGACTGCAAGGCTCTCTGCTTCATCAATTAGATACTTTGGCTTGGGGCTTGAGAATGGTAACTACACCGTGAACCTCAAGTTTGCAGAACATGTCATTCTGGCTGCTACTACCGGGAAATTTCTTGGGAGACGTCTTTTTGATATTTATATCCAGGGCATTCTGGTATCAAAGGATTTTGATATACTAAAGGAGGCAGGTATGTCATCTTTCCGAGCTGTTCAGAAGCCATACACGGCTCATGTTTCGGAGAACTACCTTGAAATCCATCTCTTTTGGGCTGGAAAGGGGACTTGCTGCGTACCTGCGCGGGGTACTTATGGACCTGCTATTGCAGCCATCAGTGCTACACCGGGTAATATAATGTGGAAATTTGAGATGCAATTTAGCATGTTTAGGTTCAGATGTTTTAAGAAGTGGTTTCACATAATCAACTAA

Protein Analysis

234

Amino Acids

26.22

Weight (kDa)

8.84

Isoelectric Point (pI)

26.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 18 - 203 6.8e-40 Malectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 48
AatII GACGTC 1 cut(s) 403
Acc36I ACCTGC 3 cut(s) 455, 580, 601
AccI GTMKAC 1 cut(s) 215
AccII CGCG 1 cut(s) 577
AciI CCGC 1 cut(s) 577
AcsI RAATTY 2 cut(s) 386, 638
AcyI GRCGYC 1 cut(s) 400
AfaI GTAC 3 cut(s) 202, 570, 583
AfiI CCNNNNNNNGG 2 cut(s) 96, 578
AflIII ACRYGT 1 cut(s) 358
AgsI TTSAA 1 cut(s) 530
AjnI CCWGG 1 cut(s) 420
AluBI AGCT 3 cut(s) 137, 222, 485
AluI AGCT 3 cut(s) 137, 222, 485
Alw26I GTCTC 1 cut(s) 391
ApeKI GCWGC 3 cut(s) 371, 564, 602
ApoI RAATTY 2 cut(s) 386, 638
AseI ATTAAT 1 cut(s) 60
AspLEI GCGC 1 cut(s) 577
AspS9I GGNCC 3 cut(s) 71, 131, 590
AsuC2I CCSGG 2 cut(s) 382, 621
AvaII GGWCC 3 cut(s) 71, 131, 590
BaeI ACNNNNGTAYC 1 cut(s) 34
BanII GRGCYC 1 cut(s) 126
BarI GAAGNNNNNNTAC 2 cut(s) 206, 238
BbsI GAAGAC 1 cut(s) 78
BbvI GCAGC 3 cut(s) 358, 551, 614
BccI CCATC 4 cut(s) 89, 159, 543, 614
BceAI ACGGC 1 cut(s) 519
BciT130I CCWGG 1 cut(s) 422
BcnI CCSGG 2 cut(s) 382, 621
BcoDI GTCTC 1 cut(s) 391
BfuAI ACCTGC 3 cut(s) 455, 580, 601
BisI GCNGC 3 cut(s) 372, 565, 603
BlsI GCNGC 3 cut(s) 373, 566, 604
Bme1390I CCNGG 3 cut(s) 382, 422, 621
Bme18I GGWCC 3 cut(s) 71, 131, 590
BmgT120I GGNCC 3 cut(s) 71, 131, 590
BmrFI CCNGG 3 cut(s) 382, 422, 621
BmsI GCATC 1 cut(s) 636
BpiI GAAGAC 1 cut(s) 78
BpuEI CTTGAG 2 cut(s) 329, 338
BpuMI CCSGG 2 cut(s) 382, 621
BsaHI GRCGYC 1 cut(s) 400
BsaJI CCNNGG 2 cut(s) 127, 421
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 578
Bse1I ACTGG 1 cut(s) 157
Bse3DI GCAATG 1 cut(s) 184
BseBI CCWGG 1 cut(s) 422
BseDI CCNNGG 2 cut(s) 127, 421
BseLI CCNNNNNNNGG 2 cut(s) 96, 578
BseMI GCAATG 1 cut(s) 184
BseMII CTCAG 2 cut(s) 66, 224
BseNI ACTGG 1 cut(s) 157
BseXI GCAGC 3 cut(s) 358, 551, 614
Bsh1236I CGCG 1 cut(s) 577
BsiSI CCGG 2 cut(s) 381, 620
BslFI GGGAC 1 cut(s) 571
BslI CCNNNNNNNGG 2 cut(s) 96, 578
BsmAI GTCTC 1 cut(s) 391
BsmBI CGTCTC 1 cut(s) 391
BsmFI GGGAC 1 cut(s) 571
BsmI GAATGC 1 cut(s) 426
Bsp1286I GDGCHC 1 cut(s) 126
BspACI CCGC 1 cut(s) 577
BspCNI CTCAG 2 cut(s) 67, 223
BspFNI CGCG 1 cut(s) 577
BspMI ACCTGC 3 cut(s) 455, 580, 601
BsrDI GCAATG 1 cut(s) 184
BsrI ACTGG 1 cut(s) 157
BssECI CCNNGG 2 cut(s) 127, 421
BssNAI GTATAC 1 cut(s) 216
BssNI GRCGYC 1 cut(s) 400
BssT1I CCWWGG 1 cut(s) 127
Bst1107I GTATAC 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 422
Bst4CI ACNGT 1 cut(s) 337
BstACI GRCGYC 1 cut(s) 400
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 2 cut(s) 75, 210
BstFNI CGCG 1 cut(s) 577
BstHHI GCGC 1 cut(s) 577
BstMAI GTCTC 1 cut(s) 391
BstMWI GCNNNNNNNGC 2 cut(s) 602, 611
BstNI CCWGG 1 cut(s) 422
BstNSI RCATGY 2 cut(s) 362, 661
BstSCI CCNGG 3 cut(s) 380, 420, 619
BstUI CGCG 1 cut(s) 577
BstV1I GCAGC 3 cut(s) 358, 551, 614
BstV2I GAAGAC 1 cut(s) 78
BstZ17I GTATAC 1 cut(s) 216
BtsIMutI CAGTG 1 cut(s) 616
BveI ACCTGC 3 cut(s) 455, 580, 601
Cac8I GCNNGC 1 cut(s) 196
CfoI GCGC 1 cut(s) 577
Cfr13I GGNCC 3 cut(s) 71, 131, 590
Csp6I GTAC 3 cut(s) 201, 569, 582
CviAII CATG 4 cut(s) 13, 359, 509, 658
CviQI GTAC 3 cut(s) 201, 569, 582
DdeI CTNAG 2 cut(s) 75, 210
Eco130I CCWWGG 1 cut(s) 127
Eco24I GRGCYC 1 cut(s) 126
Eco47I GGWCC 3 cut(s) 71, 131, 590
EcoO109I RGGNCCY 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 420
EcoT14I CCWWGG 1 cut(s) 127
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 127
Esp3I CGTCTC 1 cut(s) 391
FaeI CATG 4 cut(s) 16, 362, 512, 661
FaqI GGGAC 1 cut(s) 571
FatI CATG 4 cut(s) 12, 358, 508, 657
FauI CCCGC 1 cut(s) 570
FblI GTMKAC 1 cut(s) 215
Fnu4HI GCNGC 3 cut(s) 372, 565, 603
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 3 cut(s) 372, 565, 603
GlaI GCGC 1 cut(s) 576
GluI GCNGC 3 cut(s) 372, 565, 603
HapII CCGG 2 cut(s) 381, 620
HhaI GCGC 1 cut(s) 577
Hin1I GRCGYC 1 cut(s) 400
Hin1II CATG 4 cut(s) 16, 362, 512, 661
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HindIII AAGCTT 1 cut(s) 220
HpaII CCGG 2 cut(s) 381, 620
Hpy166II GTNNAC 3 cut(s) 81, 216, 340
Hpy188I TCNGA 6 cut(s) 94, 185, 482, 492, 517, 671
Hpy188III TCNNGA 1 cut(s) 268
Hpy8I GTNNAC 3 cut(s) 81, 216, 340
HpyAV CCTTC 1 cut(s) 106
HpyCH4III ACNGT 1 cut(s) 337
HpyCH4IV ACGT 2 cut(s) 83, 400
HpyCH4V TGCA 4 cut(s) 275, 353, 602, 649
HpyF10VI GCNNNNNNNGC 2 cut(s) 602, 611
HpyF3I CTNAG 2 cut(s) 75, 210
HpySE526I ACGT 2 cut(s) 83, 400
Hsp92I GRCGYC 1 cut(s) 400
Hsp92II CATG 4 cut(s) 16, 362, 512, 661
HspAI GCGC 1 cut(s) 575
Lsp1109I GCAGC 3 cut(s) 358, 551, 614
LweI GCATC 1 cut(s) 636
MaeII ACGT 2 cut(s) 83, 400
MaeIII GTNAC 2 cut(s) 148, 326
MboII GAAGA 1 cut(s) 78
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 7 cut(s) 49, 61, 236, 294, 386, 638, 650
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 5 cut(s) 62, 100, 219, 353, 454
MseI TTAA 2 cut(s) 60, 678
MspI CCGG 2 cut(s) 381, 620
MspR9I CCNGG 3 cut(s) 382, 422, 621
Mva1269I GAATGC 1 cut(s) 426
MvaI CCWGG 1 cut(s) 422
MvnI CGCG 1 cut(s) 577
MwoI GCNNNNNNNGC 2 cut(s) 602, 611
NciI CCSGG 2 cut(s) 382, 621
NlaIII CATG 4 cut(s) 16, 362, 512, 661
NmuCI GTSAC 1 cut(s) 148
NspI RCATGY 2 cut(s) 362, 661
PciI ACATGT 1 cut(s) 358
PctI GAATGC 1 cut(s) 426
PkrI GCNGC 3 cut(s) 373, 566, 604
PpuMI RGGWCCY 1 cut(s) 71
PscI ACATGT 1 cut(s) 358
PshBI ATTAAT 1 cut(s) 60
PsiI TTATAA 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 71
Psp6I CCWGG 1 cut(s) 420
PspGI CCWGG 1 cut(s) 420
PspPI GGNCC 3 cut(s) 71, 131, 590
PspPPI RGGWCCY 1 cut(s) 71
RsaI GTAC 3 cut(s) 202, 570, 583
RsaNI GTAC 3 cut(s) 201, 569, 582
SaqAI TTAA 2 cut(s) 60, 678
SatI GCNGC 3 cut(s) 372, 565, 603
Sau96I GGNCC 3 cut(s) 71, 131, 590
ScrFI CCNGG 3 cut(s) 382, 422, 621
SduI GDGCHC 1 cut(s) 126
SfaNI GCATC 1 cut(s) 636
SinI GGWCC 3 cut(s) 71, 131, 590
SmlI CTYRAG 2 cut(s) 317, 344
SmoI CTYRAG 2 cut(s) 317, 344
Sse9I AATT 7 cut(s) 49, 61, 236, 294, 386, 638, 650
SsiI CCGC 1 cut(s) 577
StyD4I CCNGG 3 cut(s) 380, 420, 619
StyI CCWWGG 1 cut(s) 127
TaaI ACNGT 1 cut(s) 337
TaiI ACGT 2 cut(s) 86, 403
TasI AATT 7 cut(s) 49, 61, 236, 294, 386, 638, 650
Tru1I TTAA 2 cut(s) 60, 678
Tru9I TTAA 2 cut(s) 60, 678
TscAI CASTG 1 cut(s) 616
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 3 cut(s) 371, 564, 602
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 2 cut(s) 129, 279
TspRI CASTG 1 cut(s) 616
VpaK11BI GGWCC 3 cut(s) 71, 131, 590
VspI ATTAAT 1 cut(s) 60
XapI RAATTY 2 cut(s) 386, 638
XceI RCATGY 2 cut(s) 362, 661
XmiI GTMKAC 1 cut(s) 215
ZraI GACGTC 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.