Rmu_sc0024030.1_g000007

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0024030.1
OG0001780
Physical Location & Seq
Reverse (-)
45691 .. 47022
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0024030.1_g000007.1.cds

Sequence Viewer

Length: 750 bp
atgttatggagggatcaagcccagactagtgctgccattgttactggtgccatggcctggcacgaggagttcttgcaatctcatcgatcgttgatcatttctcagcaaggcagggagaagccccggggtgtgcatagaaattcacaaggccaatttcttgcgagcttttcacatataatggattttgtcagctcccttttgcatgtcaaacttttagctttgaagaacggactagagttgctgcaatccatgtttattgatcaagccatcctcaaaagtgactgcttacttgcagtacaggctattctttctccagctgcagacctctcttgtctgggtatgttgatcatggatataaagactattctagcagcaaatgagaacttcagaatctgctttgctccccgtcaggctaatgtcgtagctcatagccaaagagaaatctgtgcagttggacacaattttcaagggcgcgaccttatcgacgtcgatgcttgcaacgggaggattgggctgacgatgatgagaaccgtagggaacgagagagctgaggctgatgatgcctccgtggccggggatggcgacgcggagagagtagaaaaggcagggcaacacggtgctgactctggagacaacgaggttgtcggtggaaaggcggagagcactggatgggaggagcgtggggaggatggagccgaaggagatagatcggagttggagagagagagagagagaggttttataacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

249

Amino Acids

27.19

Weight (kDa)

5.11

Isoelectric Point (pI)

33.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 743
AatII GACGTC 1 cut(s) 489
AccB1I GGYRCC 1 cut(s) 47
AccB7I CCANNNNNTGG 1 cut(s) 57
AccII CGCG 2 cut(s) 474, 587
AciI CCGC 2 cut(s) 587, 656
AclWI GGATC 1 cut(s) 21
AcoI YGGCCR 1 cut(s) 570
AcsI RAATTY 1 cut(s) 139
AcuI CTGAAG 1 cut(s) 370
AcyI GRCGYC 1 cut(s) 486
AfaI GTAC 1 cut(s) 297
AfiI CCNNNNNNNGG 2 cut(s) 57, 573
AgsI TTSAA 2 cut(s) 223, 467
AhlI ACTAGT 1 cut(s) 26
AjnI CCWGG 1 cut(s) 56
AluBI AGCT 6 cut(s) 165, 192, 218, 317, 425, 548
AluI AGCT 6 cut(s) 165, 192, 218, 317, 425, 548
Alw21I GWGCWC 1 cut(s) 665
Alw26I GTCTC 1 cut(s) 624
AlwI GGATC 1 cut(s) 21
AlwNI CAGNNNCTG 1 cut(s) 393
Ama87I CYCGRG 1 cut(s) 123
AoxI GGCC 3 cut(s) 54, 148, 570
ApeKI GCWGC 4 cut(s) 32, 241, 317, 371
ApoI RAATTY 1 cut(s) 139
AspLEI GCGC 1 cut(s) 474
AsuC2I CCSGG 3 cut(s) 124, 125, 574
AvaI CYCGRG 1 cut(s) 123
BanI GGYRCC 1 cut(s) 47
BauI CACGAG 1 cut(s) 62
Bbv12I GWGCWC 1 cut(s) 665
BbvCI CCTCAGC 1 cut(s) 549
BbvI GCAGC 4 cut(s) 19, 228, 304, 383
BccI CCATC 4 cut(s) 275, 572, 663, 683
BcgI CGANNNNNNTGC 4 cut(s) 65, 99, 473, 507
BciT130I CCWGG 1 cut(s) 58
BclI TGATCA 3 cut(s) 93, 259, 345
BcnI CCSGG 3 cut(s) 124, 125, 574
BcoDI GTCTC 1 cut(s) 624
BcuI ACTAGT 1 cut(s) 26
BfaI CTAG 3 cut(s) 27, 233, 368
BfmI CTRYAG 1 cut(s) 318
BglI GCCNNNNNGGC 1 cut(s) 569
BisI GCNGC 4 cut(s) 33, 242, 318, 372
BlsI GCNGC 4 cut(s) 34, 243, 319, 373
Bme1390I CCNGG 4 cut(s) 58, 124, 125, 574
BmeT110I CYCGRG 1 cut(s) 123
BmiI GGNNCC 2 cut(s) 49, 694
BmrFI CCNGG 4 cut(s) 58, 124, 125, 574
BmsI GCATC 2 cut(s) 481, 550
BpmI CTGGAG 2 cut(s) 297, 648
Bpu10I CCTNAGC 1 cut(s) 549
BpuMI CCSGG 3 cut(s) 124, 125, 574
Bsa29I ATCGAT 1 cut(s) 85
BsaHI GRCGYC 1 cut(s) 486
BsaJI CCNNGG 6 cut(s) 51, 122, 123, 124, 567, 573
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 573
Bse1I ACTGG 2 cut(s) 49, 670
BseBI CCWGG 1 cut(s) 58
BseCI ATCGAT 1 cut(s) 85
BseDI CCNNGG 6 cut(s) 51, 122, 123, 124, 567, 573
BseGI GGATG 4 cut(s) 267, 583, 674, 694
BseLI CCNNNNNNNGG 2 cut(s) 57, 573
BseMII CTCAG 2 cut(s) 116, 540
BseNI ACTGG 2 cut(s) 49, 670
BseRI GAGGAG 2 cut(s) 80, 689
BseXI GCAGC 4 cut(s) 19, 228, 304, 383
BsgI GTGCAG 1 cut(s) 468
Bsh1236I CGCG 2 cut(s) 474, 587
Bsh1285I CGRYCG 1 cut(s) 89
BshFI GGCC 3 cut(s) 56, 150, 572
BshNI GGYRCC 1 cut(s) 47
BshVI ATCGAT 1 cut(s) 85
BsiEI CGRYCG 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 665
BsiHKCI CYCGRG 1 cut(s) 123
BsiSI CCGG 2 cut(s) 124, 573
BslI CCNNNNNNNGG 2 cut(s) 57, 573
BsmAI GTCTC 1 cut(s) 624
BsnI GGCC 3 cut(s) 56, 150, 572
BsoBI CYCGRG 1 cut(s) 123
Bsp1286I GDGCHC 1 cut(s) 665
Bsp143I GATC 6 cut(s) 13, 86, 93, 259, 345, 707
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 2 cut(s) 587, 656
BspANI GGCC 3 cut(s) 56, 150, 572
BspCNI CTCAG 2 cut(s) 115, 541
BspDI ATCGAT 1 cut(s) 85
BspFNI CGCG 2 cut(s) 474, 587
BspLI GGNNCC 2 cut(s) 49, 694
BspMAI CTGCAG 1 cut(s) 322
BspPI GGATC 1 cut(s) 21
BspT107I GGYRCC 1 cut(s) 47
BsrI ACTGG 2 cut(s) 49, 670
BssECI CCNNGG 6 cut(s) 51, 122, 123, 124, 567, 573
BssMI GATC 6 cut(s) 13, 86, 93, 259, 345, 707
BssNI GRCGYC 1 cut(s) 486
BssSI CACGAG 1 cut(s) 62
BssT1I CCWWGG 1 cut(s) 51
Bst2BI CACGAG 1 cut(s) 62
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 2 cut(s) 532, 617
BstACI GRCGYC 1 cut(s) 486
BstC8I GCNNGC 2 cut(s) 163, 496
BstDEI CTNAG 2 cut(s) 102, 549
BstDSI CCRYGG 2 cut(s) 51, 567
BstF5I GGATG 4 cut(s) 267, 583, 674, 694
BstFNI CGCG 2 cut(s) 474, 587
BstHHI GCGC 1 cut(s) 474
BstKTI GATC 6 cut(s) 16, 89, 96, 262, 348, 710
BstMAI GTCTC 1 cut(s) 624
BstMBI GATC 6 cut(s) 13, 86, 93, 259, 345, 707
BstMCI CGRYCG 1 cut(s) 89
BstMWI GCNNNNNNNGC 3 cut(s) 299, 560, 569
BstNI CCWGG 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 206
BstSCI CCNGG 4 cut(s) 56, 122, 123, 572
BstSFI CTRYAG 1 cut(s) 318
BstUI CGCG 2 cut(s) 474, 587
BstV1I GCAGC 4 cut(s) 19, 228, 304, 383
Bsu15I ATCGAT 1 cut(s) 85
BsuRI GGCC 3 cut(s) 56, 150, 572
BsuTUI ATCGAT 1 cut(s) 85
BtgI CCRYGG 2 cut(s) 51, 567
BtsCI GGATG 4 cut(s) 267, 583, 674, 694
BtsIMutI CAGTG 1 cut(s) 663
Cac8I GCNNGC 2 cut(s) 163, 496
CaiI CAGNNNCTG 1 cut(s) 393
CfoI GCGC 1 cut(s) 474
Cfr9I CCCGGG 1 cut(s) 123
ClaI ATCGAT 1 cut(s) 85
CseI GACGC 1 cut(s) 593
Csp6I GTAC 1 cut(s) 296
CviAII CATG 5 cut(s) 52, 203, 250, 349, 747
CviQI GTAC 1 cut(s) 296
DdeI CTNAG 2 cut(s) 102, 549
DpnI GATC 6 cut(s) 15, 88, 95, 261, 347, 709
DpnII GATC 6 cut(s) 13, 86, 93, 259, 345, 707
EaeI YGGCCR 1 cut(s) 570
EciI GGCGGA 1 cut(s) 671
Eco130I CCWWGG 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 370
Eco88I CYCGRG 1 cut(s) 123
EcoRII CCWGG 1 cut(s) 56
EcoT14I CCWWGG 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 5 cut(s) 55, 206, 253, 352, 750
FatI CATG 5 cut(s) 51, 202, 249, 348, 746
FbaI TGATCA 3 cut(s) 93, 259, 345
Fnu4HI GCNGC 4 cut(s) 33, 242, 318, 372
FokI GGATG 4 cut(s) 254, 590, 681, 701
Fsp4HI GCNGC 4 cut(s) 33, 242, 318, 372
FspBI CTAG 3 cut(s) 27, 233, 368
GlaI GCGC 1 cut(s) 473
GluI GCNGC 4 cut(s) 33, 242, 318, 372
GsuI CTGGAG 2 cut(s) 297, 648
HaeIII GGCC 3 cut(s) 56, 150, 572
HapII CCGG 2 cut(s) 124, 573
HgaI GACGC 1 cut(s) 593
HhaI GCGC 1 cut(s) 474
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 5 cut(s) 55, 206, 253, 352, 750
Hin6I GCGC 1 cut(s) 472
HinP1I GCGC 1 cut(s) 472
HinfI GANTC 2 cut(s) 390, 623
HpaII CCGG 2 cut(s) 124, 573
Hpy188I TCNGA 2 cut(s) 389, 712
Hpy188III TCNNGA 1 cut(s) 627
Hpy99I CGWCG 3 cut(s) 488, 491, 587
HpyAV CCTTC 1 cut(s) 692
HpyCH4III ACNGT 2 cut(s) 532, 617
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 8 cut(s) 76, 133, 202, 244, 293, 320, 449, 498
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 560, 569
HpyF3I CTNAG 2 cut(s) 102, 549
HpySE526I ACGT 1 cut(s) 486
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 5 cut(s) 55, 206, 253, 352, 750
HspAI GCGC 1 cut(s) 472
Ksp22I TGATCA 3 cut(s) 93, 259, 345
Kzo9I GATC 6 cut(s) 13, 86, 93, 259, 345, 707
LmnI GCTCC 4 cut(s) 197, 406, 676, 692
Lsp1109I GCAGC 4 cut(s) 19, 228, 304, 383
LweI GCATC 2 cut(s) 481, 550
MaeI CTAG 3 cut(s) 27, 233, 368
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 2 cut(s) 40, 278
MalI GATC 6 cut(s) 15, 88, 95, 261, 347, 709
MboI GATC 6 cut(s) 13, 86, 93, 259, 345, 707
MboII GAAGA 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 665
MluCI AATT 3 cut(s) 139, 152, 460
MlyI GAGTC 1 cut(s) 617
MmeI TCCRAC 2 cut(s) 433, 696
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 2 cut(s) 124, 573
MspR9I CCNGG 4 cut(s) 58, 124, 125, 574
MvaI CCWGG 1 cut(s) 58
MvnI CGCG 2 cut(s) 474, 587
MwoI GCNNNNNNNGC 3 cut(s) 299, 560, 569
NciI CCSGG 3 cut(s) 124, 125, 574
NcoI CCATGG 1 cut(s) 51
NdeII GATC 6 cut(s) 13, 86, 93, 259, 345, 707
NlaIII CATG 5 cut(s) 55, 206, 253, 352, 750
NlaIV GGNNCC 2 cut(s) 49, 694
NmuCI GTSAC 1 cut(s) 278
NspI RCATGY 1 cut(s) 206
PfeI GAWTC 1 cut(s) 390
PflMI CCANNNNNTGG 1 cut(s) 57
PkrI GCNGC 4 cut(s) 34, 243, 319, 373
Ple19I CGATCG 1 cut(s) 89
PleI GAGTC 1 cut(s) 617
PpsI GAGTC 1 cut(s) 617
PsiI TTATAA 1 cut(s) 743
Psp6I CCWGG 1 cut(s) 56
PspGI CCWGG 1 cut(s) 56
PspN4I GGNNCC 2 cut(s) 49, 694
PstI CTGCAG 1 cut(s) 322
PstNI CAGNNNCTG 1 cut(s) 393
PvuI CGATCG 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 317
RsaI GTAC 1 cut(s) 297
RsaNI GTAC 1 cut(s) 296
SatI GCNGC 4 cut(s) 33, 242, 318, 372
Sau3AI GATC 6 cut(s) 13, 86, 93, 259, 345, 707
SchI GAGTC 1 cut(s) 617
ScrFI CCNGG 4 cut(s) 58, 124, 125, 574
SduI GDGCHC 1 cut(s) 665
SfaNI GCATC 2 cut(s) 481, 550
SfcI CTRYAG 1 cut(s) 318
SmaI CCCGGG 1 cut(s) 125
SpeI ACTAGT 1 cut(s) 26
Sse9I AATT 3 cut(s) 139, 152, 460
SsiI CCGC 2 cut(s) 587, 656
SspMI CTAG 3 cut(s) 27, 233, 368
StyD4I CCNGG 4 cut(s) 56, 122, 123, 572
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 2 cut(s) 532, 617
TaiI ACGT 1 cut(s) 489
TaqI TCGA 3 cut(s) 85, 483, 489
TasI AATT 3 cut(s) 139, 152, 460
TatI WGTACW 1 cut(s) 295
TfiI GAWTC 1 cut(s) 390
TscAI CASTG 1 cut(s) 670
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 4 cut(s) 32, 241, 317, 371
Tsp45I GTSAC 1 cut(s) 278
TspGWI ACGGA 2 cut(s) 243, 556
TspMI CCCGGG 1 cut(s) 123
TspRI CASTG 1 cut(s) 670
Van91I CCANNNNNTGG 1 cut(s) 57
XapI RAATTY 1 cut(s) 139
XceI RCATGY 1 cut(s) 206
XmaI CCCGGG 1 cut(s) 123
XspI CTAG 3 cut(s) 27, 233, 368
ZraI GACGTC 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.