MD16G1054100.v1.1

Heat shock protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001272
Physical Location & Seq
Reverse (-)
3825148 .. 3830828
5681 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1054100.v1.1.491

Sequence Viewer

Length: 2385 bp
ATGGCTCCAGTTCTGAGCAGAAGCATGGCAACTGCTTCTCTGGCTTCGCTTCCTTCTTCGTCTCCATTCGCGCTCGGAAACCCCAGCAGGGCCTTGAGCCTCAGAAGCGCTTTTGTGCCGCAGAATGGGCTCCGAAAGGGCTTCTCGTGCGGCGGATTGAAGTGGAAGGTTGAGAGCAGGAACAGACGAGTGTCCGTCCGCTGCGACGCGGCGGTTGCCGAGAAGGAGGAGGCCACCGATGCCCCCGGTGAGAGATTCGAGTACCAGGCCGAGGTGTCTCGGCTTATGGATTTGATAGTTCATAGTCTATACAGCCACAAGGAGGTGTTCCTTAGAGAGCTTGTGAGTAATGCTAGTGATGCATTGGACAAGTTGAGATTTTTGAGTGTGACTGAGCCTCATCTGCTTGGCGATTCTGGTGAGCTACAGATACGCATCAAACCTGATCCAGACAATGGGACTGTCACTATCTCGGATACTGGTATTGGAATGACAAAAGAGGAACTCATTGACTGTCTTGGAACCATTGCTCAGAGTGGCACTTCAAAGTTTCTAAAGGCTCTGAAGGAAAACAAGGATCTTGGTGCAGACAATGGTTTGATTGGTCAATTTGGTGTTGGGTTCTATTCTGCTTTTCTTGTTGCTGACAAGGTGGTCGTCTCTACAAAAAGCCCAAGGTCAGACAAGCAATATGTTTGGGAAGCAGCAGCTGACAGTAGCTCTTATGTTATTAAGGAAGAAACTAATCCCGAAAATCTCATACAACGTGGAACACAGATTACCCTCTATTTAAGGCCGGATGACAAGTATGAATTCTCAGAGCCGGCCCGGATCCAGGGTTTAGTGAAGAATTACTCACAGTTTGTTTCCTTCCCCATCTATACATGGCAAGAAAAATCTAGGACTGTAGAGGTTGAAGAGGAGGAAGAACCAAAAGAAGGAGAAGAACCAAAACCAGAGGGTGAGAAGAAAAAGAAGACAAAAACTGAGAAGTATTGGGATTGGGAATTAGCCAATGAAACAAAGCCTATATGGATGCGGAGTCCAAAGGAAGTTCAAAAAGAGGAGTATCAAGAATTTTACAAGAAAACTTTTAGTGAGTTCCTGGATCCACTTGGATACACTCACTTCACCACTGAGGGTGAGGTGGAGTTCAGGAGTGTTCTTTATATTCCTGGAATGGGGCCTCTTAACAATGAAGATGTAGTAAATCCTAAAACAAAGAATATACGTTTGTACGTGAAGCGGGTATTTATCTCAGATGATTTTGATGGTGAACTGTTTCCGCGATACCTGAGCTTTGTTAAGGGTGTGGTGGATTCAAATGATCTTCCTCTTAATGTTTCTCGCGAGATTCTTCAAGAAAGTCGAATTGTAAGAATCATGAGAAAGAGACTTGTGCGAAAGACATTTGACATGATTCAAGATATTTCTGAGAGTGAAAATAAAGAGGATTACAAGAAACTATGGGAGAACTTTGGCAGGTTCATAAAGTTAGGTTGCATTGAGGACTCTGGAAATCACAAGCGCATAACACCCCTGTTGCGGTTCTACTCTTCAAAAAGTGGGGAAGAACTGATAAGCTTAGATGATTATGTTGAAAACATGCCTGAGAACCAGAAGGCAATCTATTACTTGGCATCAGACAGTCTACAAAGTGCAAAAAGTGCCCCTTTCTTGGAAAAGTTGGTCCAAAAAGGCATTGAGGTTTTGTATTTAGTTGAACCTATTGATGAAGTGGCTATCCAGAATCTACAAACATACAAAGAAAAGAAGTTTGTAGACATCAGCAAGGAAGATTTGGAGCTTGGTGACGAAGATGAGGTCAAAGAAAGGGAAACTGAACAACAATACACTCTTGTGTGCGACTGGATAAAGCAGCAACTTGGTGATAAGGTGGCAAAAGTCCAAGTCTCGAAGCGTTTAAGCTCATCTCCCTGTGTACTTGTTTCTGGAAAGTTTGGATGGTCTGCCAACATGGAGAGATTGATGAAAGCACAGGCCCTTGGAGACACTGCAAGTTTGGAGTTCATGAGGGGCAGGAGAATATTGGAAATTAACCCAGATCATCCCATCGTTAAGGACCTAAAGGCTGCTTGCAAGAATGCCCCTGACAGCACTGATGCCAAGAGAGCTGTAGAACTCTTGTATGACACAGCATTGATCTCCAGTGGATTCACGCCCGACAGCCCAGCTGATTTGGGAAACAAGATATACGAGATGATGGCAATGGCCCTCGGGGGAAGATGGGGTAGATTGGAAGATGAGGAGGTGGCCGACGGTACTAATGGGGCAGAATCCGATGCGAATGCCGCTGAAGCGGTGGAAGCACAAGTAGTTGAAGCATCAGAAGTGAGGACAGAGAGTGATCCATGGAGCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0001101 GO:0003674 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005783 GO:0005788 GO:0006605 GO:0006810 GO:0006886 GO:0006950 GO:0006970 GO:0007275 GO:0008104 GO:0008144 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009639 GO:0009640 GO:0009651 GO:0009704 GO:0009791 GO:0009941 GO:0010035 GO:0010157 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0017076 GO:0030554 GO:0031090 GO:0031967 GO:0031974 GO:0031975 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033365 GO:0034613 GO:0035639 GO:0036094 GO:0042221 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044432 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0046983 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0070013 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0072594 GO:0072596 GO:0072598 GO:0097159 GO:0097367 GO:0098588 GO:0098805 GO:1901265 GO:1901363 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04141 ko04151 ko04626 ko04657 ko04915 ko04918 ko05200 ko05215 ko05418 map04141 map04151 map04626 map04657 map04915 map04918 map05200 map05215 map05418
Pfam Domains
Protein Families
HATPase_c HSP90

Protein Analysis

795

Amino Acids

89.64

Weight (kDa)

5.02

Isoelectric Point (pI)

45.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HATPase_c_3 PF13589 108 - 248 1.6e-08 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
HATPase_c PF02518 109 - 265 1e-06 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
HSP90 PF00183 271 - 766 2.4e-194 Hsp90 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 653
Acc36I ACCTGC 1 cut(s) 1473
AccB7I CCANNNNNTGG 1 cut(s) 455
AccI GTMKAC 2 cut(s) 1651, 1782
AccII CGCG 4 cut(s) 71, 209, 1288, 1350
AclWI GGATC 7 cut(s) 440, 585, 826, 839, 1103, 1116, 2363
AcoI YGGCCR 1 cut(s) 2274
AcsI RAATTY 2 cut(s) 812, 1076
AcuI CTGAAG 2 cut(s) 584, 2337
AfaI GTAC 4 cut(s) 263, 1238, 1944, 2284
AfeI AGCGCT 1 cut(s) 109
AjnI CCWGG 4 cut(s) 264, 834, 1104, 1174
AleI CACNNNNGTG 1 cut(s) 1859
Alw26I GTCTC 6 cut(s) 66, 282, 664, 1387, 1918, 2004
AlwI GGATC 7 cut(s) 440, 585, 826, 839, 1103, 1116, 2363
AlwNI CAGNNNCTG 1 cut(s) 710
Ama87I CYCGRG 1 cut(s) 2237
Aor51HI AGCGCT 1 cut(s) 109
AoxI GGCC 9 cut(s) 90, 231, 267, 794, 825, 1184, 2001, 2232, 2274
ApeKI GCWGC 5 cut(s) 201, 704, 707, 1879, 2093
ApoI RAATTY 2 cut(s) 812, 1076
ArsI GACNNNNNNTTYG 2 cut(s) 1809, 1841
Asp700I GAANNNNTTC 1 cut(s) 1281
AspLEI GCGC 3 cut(s) 73, 110, 1530
AspS9I GGNCC 7 cut(s) 90, 826, 1184, 1690, 2002, 2083, 2233
AsuC2I CCSGG 2 cut(s) 246, 829
AsuHPI GGTGA 8 cut(s) 260, 431, 974, 1123, 1154, 1286, 1823, 1901
AvaI CYCGRG 1 cut(s) 2237
AvaII GGWCC 2 cut(s) 1690, 2083
BaeGI GKGCMC 1 cut(s) 1672
BamHI GGATCC 2 cut(s) 831, 1108
BanII GRGCYC 1 cut(s) 132
BauI CACGAG 1 cut(s) 145
BbsI GAAGAC 1 cut(s) 983
BbvI GCAGC 5 cut(s) 188, 716, 719, 1891, 2080
BccI CCATC 6 cut(s) 884, 1265, 1959, 2081, 2218, 2241
BcgI CGANNNNNNTGC 2 cut(s) 2291, 2325
BciT130I CCWGG 4 cut(s) 266, 836, 1106, 1176
BciVI GTATCC 2 cut(s) 469, 1112
BcnI CCSGG 2 cut(s) 246, 829
BcoDI GTCTC 6 cut(s) 66, 282, 664, 1387, 1918, 2004
BfaI CTAG 2 cut(s) 354, 900
BfmI CTRYAG 3 cut(s) 425, 906, 2136
BfoI RGCGCY 1 cut(s) 111
BfuAI ACCTGC 1 cut(s) 1473
BfuI GTATCC 2 cut(s) 469, 1112
BisI GCNGC 9 cut(s) 119, 151, 202, 210, 705, 708, 1880, 2094, 2313
BlsI GCNGC 9 cut(s) 120, 152, 203, 211, 706, 709, 1881, 2095, 2314
Bme1390I CCNGG 6 cut(s) 246, 266, 829, 836, 1106, 1176
Bme18I GGWCC 2 cut(s) 1690, 2083
BmeT110I CYCGRG 1 cut(s) 2237
BmgT120I GGNCC 7 cut(s) 90, 826, 1184, 1690, 2002, 2083, 2233
BmiI GGNNCC 6 cut(s) 6, 131, 523, 833, 1110, 1185
BmrFI CCNGG 6 cut(s) 246, 266, 829, 836, 1106, 1176
BmsI GCATC 8 cut(s) 229, 349, 444, 1026, 1649, 2113, 2293, 2354
BoxI GACNNNNGTC 1 cut(s) 190
BpiI GAAGAC 1 cut(s) 983
BpmI CTGGAG 1 cut(s) 2152
Bpu10I CCTNAGC 1 cut(s) 1295
BpuEI CTTGAG 1 cut(s) 115
BpuMI CCSGG 2 cut(s) 246, 829
BsaAI YACGTR 1 cut(s) 1240
BsaBI GATNNNNATC 1 cut(s) 434
BsaJI CCNNGG 7 cut(s) 244, 270, 674, 835, 2005, 2236, 2372
Bse118I RCCGGY 1 cut(s) 823
Bse1I ACTGG 4 cut(s) 8, 484, 1874, 2169
Bse3DI GCAATG 2 cut(s) 525, 2235
Bse8I GATNNNNATC 1 cut(s) 434
BseBI CCWGG 4 cut(s) 266, 836, 1106, 1176
BseDI CCNNGG 7 cut(s) 244, 270, 674, 835, 2005, 2236, 2372
BseGI GGATG 4 cut(s) 805, 1041, 1970, 2068
BseJI GATNNNNATC 1 cut(s) 434
BseMI GCAATG 2 cut(s) 525, 2235
BseNI ACTGG 4 cut(s) 8, 484, 1874, 2169
BseRI GAGGAG 4 cut(s) 242, 935, 1079, 2282
BseSI GKGCMC 1 cut(s) 1672
BseXI GCAGC 5 cut(s) 188, 716, 719, 1891, 2080
BseYI CCCAGC 2 cut(s) 83, 2191
BsgI GTGCAG 1 cut(s) 606
Bsh1236I CGCG 4 cut(s) 71, 209, 1288, 1350
BshFI GGCC 9 cut(s) 92, 233, 269, 796, 827, 1186, 2003, 2234, 2276
BsiHKCI CYCGRG 1 cut(s) 2237
BsiSI CCGG 4 cut(s) 246, 797, 824, 829
BslFI GGGAC 1 cut(s) 472
BsmAI GTCTC 6 cut(s) 66, 282, 664, 1387, 1918, 2004
BsmBI CGTCTC 2 cut(s) 66, 664
BsmFI GGGAC 1 cut(s) 472
BsmI GAATGC 2 cut(s) 2110, 2314
BsnI GGCC 9 cut(s) 92, 233, 269, 796, 827, 1186, 2003, 2234, 2276
BsoBI CYCGRG 1 cut(s) 2237
Bsp1286I GDGCHC 2 cut(s) 132, 1672
Bsp143I GATC 8 cut(s) 445, 577, 831, 1108, 1327, 2065, 2163, 2368
Bsp19I CCATGG 1 cut(s) 2372
Bsp68I TCGCGA 1 cut(s) 1350
BspANI GGCC 9 cut(s) 92, 233, 269, 796, 827, 1186, 2003, 2234, 2276
BspFNI CGCG 4 cut(s) 71, 209, 1288, 1350
BspHI TCATGA 2 cut(s) 1383, 2031
BspLI GGNNCC 6 cut(s) 6, 131, 523, 833, 1110, 1185
BspMI ACCTGC 1 cut(s) 1473
BspPI GGATC 7 cut(s) 440, 585, 826, 839, 1103, 1116, 2363
BsrDI GCAATG 2 cut(s) 525, 2235
BsrFI RCCGGY 1 cut(s) 823
BsrI ACTGG 4 cut(s) 8, 484, 1874, 2169
BssAI RCCGGY 1 cut(s) 823
BssECI CCNNGG 7 cut(s) 244, 270, 674, 835, 2005, 2236, 2372
BssMI GATC 8 cut(s) 445, 577, 831, 1108, 1327, 2065, 2163, 2368
BssSI CACGAG 1 cut(s) 145
BssT1I CCWWGG 3 cut(s) 674, 2005, 2372
Bst2BI CACGAG 1 cut(s) 145
Bst2UI CCWGG 4 cut(s) 266, 836, 1106, 1176
Bst4CI ACNGT 8 cut(s) 463, 515, 716, 861, 907, 1281, 1649, 2282
Bst6I CTCTTC 2 cut(s) 912, 1561
BstAPI GCANNNNNTGC 1 cut(s) 1667
BstBAI YACGTR 1 cut(s) 1240
BstC8I GCNNGC 2 cut(s) 825, 2098
BstDSI CCRYGG 1 cut(s) 2372
BstF5I GGATG 4 cut(s) 805, 1041, 1970, 2068
BstFNI CGCG 4 cut(s) 71, 209, 1288, 1350
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 3 cut(s) 73, 110, 1530
BstKTI GATC 8 cut(s) 448, 580, 834, 1111, 1330, 2068, 2166, 2371
BstMAI GTCTC 6 cut(s) 66, 282, 664, 1387, 1918, 2004
BstMBI GATC 8 cut(s) 445, 577, 831, 1108, 1327, 2065, 2163, 2368
BstNI CCWGG 4 cut(s) 266, 836, 1106, 1176
BstNSI RCATGY 1 cut(s) 1609
BstPAI GACNNNNGTC 1 cut(s) 190
BstSCI CCNGG 6 cut(s) 244, 264, 827, 834, 1104, 1174
BstSFI CTRYAG 3 cut(s) 425, 906, 2136
BstSLI GKGCMC 1 cut(s) 1672
BstUI CGCG 4 cut(s) 71, 209, 1288, 1350
BstV1I GCAGC 5 cut(s) 188, 716, 719, 1891, 2080
BstV2I GAAGAC 1 cut(s) 983
BstX2I RGATCY 3 cut(s) 577, 831, 1108
BstYI RGATCY 3 cut(s) 577, 831, 1108
BsuI GTATCC 2 cut(s) 469, 1112
BsuRI GGCC 9 cut(s) 92, 233, 269, 796, 827, 1186, 2003, 2234, 2276
BtgI CCRYGG 1 cut(s) 2372
BtsCI GGATG 4 cut(s) 805, 1041, 1970, 2068
BtsI GCAGTG 1 cut(s) 2013
BtsIMutI CAGTG 4 cut(s) 1134, 2013, 2118, 2176
BtuMI TCGCGA 1 cut(s) 1350
BveI ACCTGC 1 cut(s) 1473
Cac8I GCNNGC 2 cut(s) 825, 2098
CaiI CAGNNNCTG 1 cut(s) 710
CciI TCATGA 2 cut(s) 1383, 2031
CfoI GCGC 3 cut(s) 73, 110, 1530
Cfr10I RCCGGY 1 cut(s) 823
Cfr13I GGNCC 7 cut(s) 90, 826, 1184, 1690, 2002, 2083, 2233
CseI GACGC 1 cut(s) 215
Csp6I GTAC 4 cut(s) 262, 1237, 1943, 2283
CviAII CATG 8 cut(s) 25, 885, 1384, 1417, 1606, 1978, 2032, 2373
CviQI GTAC 4 cut(s) 262, 1237, 1943, 2283
DpnI GATC 8 cut(s) 447, 579, 833, 1110, 1329, 2067, 2165, 2370
DpnII GATC 8 cut(s) 445, 577, 831, 1108, 1327, 2065, 2163, 2368
DrdI GACNNNNNNGTC 1 cut(s) 653
DseDI GACNNNNNNGTC 1 cut(s) 653
EaeI YGGCCR 1 cut(s) 2274
Eam1104I CTCTTC 2 cut(s) 912, 1561
EarI CTCTTC 2 cut(s) 912, 1561
EciI GGCGGA 1 cut(s) 168
Eco130I CCWWGG 3 cut(s) 674, 2005, 2372
Eco24I GRGCYC 1 cut(s) 132
Eco47I GGWCC 2 cut(s) 1690, 2083
Eco47III AGCGCT 1 cut(s) 109
Eco57I CTGAAG 2 cut(s) 584, 2337
Eco88I CYCGRG 1 cut(s) 2237
EcoO109I RGGNCCY 4 cut(s) 90, 1184, 2002, 2083
EcoRI GAATTC 1 cut(s) 812
EcoRII CCWGG 4 cut(s) 264, 834, 1104, 1174
EcoT14I CCWWGG 3 cut(s) 674, 2005, 2372
EcoT22I ATGCAT 1 cut(s) 364
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 3 cut(s) 674, 2005, 2372
Esp3I CGTCTC 2 cut(s) 66, 664
FaeI CATG 8 cut(s) 28, 888, 1387, 1420, 1609, 1981, 2035, 2376
FalI AAGNNNNNCTT 2 cut(s) 1657, 1689
FaqI GGGAC 1 cut(s) 472
FatI CATG 8 cut(s) 24, 884, 1383, 1416, 1605, 1977, 2031, 2372
FauI CCCGC 1 cut(s) 1239
FblI GTMKAC 2 cut(s) 1651, 1782
Fnu4HI GCNGC 9 cut(s) 119, 151, 202, 210, 705, 708, 1880, 2094, 2313
FokI GGATG 4 cut(s) 812, 1048, 1977, 2055
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 9 cut(s) 119, 151, 202, 210, 705, 708, 1880, 2094, 2313
FspBI CTAG 2 cut(s) 354, 900
GlaI GCGC 3 cut(s) 72, 109, 1529
GluI GCNGC 9 cut(s) 119, 151, 202, 210, 705, 708, 1880, 2094, 2313
GsaI CCCAGC 2 cut(s) 87, 2195
GsuI CTGGAG 1 cut(s) 2152
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 9 cut(s) 92, 233, 269, 796, 827, 1186, 2003, 2234, 2276
HapII CCGG 4 cut(s) 246, 797, 824, 829
HgaI GACGC 1 cut(s) 215
HhaI GCGC 3 cut(s) 73, 110, 1530
Hin1II CATG 8 cut(s) 28, 888, 1387, 1420, 1609, 1981, 2035, 2376
Hin6I GCGC 3 cut(s) 71, 108, 1528
HinP1I GCGC 3 cut(s) 71, 108, 1528
HindIII AAGCTT 1 cut(s) 1582
HpaII CCGG 4 cut(s) 246, 797, 824, 829
HphI GGTGA 8 cut(s) 260, 431, 974, 1123, 1154, 1286, 1823, 1901
Hpy166II GTNNAC 4 cut(s) 1277, 1652, 1783, 1943
Hpy8I GTNNAC 4 cut(s) 1277, 1652, 1783, 1943
Hpy99I CGWCG 2 cut(s) 209, 2282
HpyAV CCTTC 7 cut(s) 63, 160, 217, 559, 880, 932, 1615
HpyCH4III ACNGT 8 cut(s) 463, 515, 716, 861, 907, 1281, 1649, 2282
HpyCH4IV ACGT 3 cut(s) 766, 1231, 1239
HpyCH4V TGCA 6 cut(s) 362, 587, 1503, 1661, 2018, 2100
HpySE526I ACGT 3 cut(s) 766, 1231, 1239
Hsp92II CATG 8 cut(s) 28, 888, 1387, 1420, 1609, 1981, 2035, 2376
HspAI GCGC 3 cut(s) 71, 108, 1528
KroI GCCGGC 1 cut(s) 823
KroNI GCCGGC 1 cut(s) 825
Kzo9I GATC 8 cut(s) 445, 577, 831, 1108, 1327, 2065, 2163, 2368
LmnI GCTCC 4 cut(s) 10, 135, 1804, 2376
Lsp1109I GCAGC 5 cut(s) 188, 716, 719, 1891, 2080
LweI GCATC 8 cut(s) 229, 349, 444, 1026, 1649, 2113, 2293, 2354
MaeI CTAG 2 cut(s) 354, 900
MaeII ACGT 3 cut(s) 766, 1231, 1239
MaeIII GTNAC 3 cut(s) 388, 463, 1811
MalI GATC 8 cut(s) 447, 579, 833, 1110, 1329, 2067, 2165, 2370
MboI GATC 8 cut(s) 445, 577, 831, 1108, 1327, 2065, 2163, 2368
MflI RGATCY 3 cut(s) 577, 831, 1108
MhlI GDGCHC 2 cut(s) 132, 1672
MluCI AATT 7 cut(s) 608, 812, 850, 1007, 1076, 1371, 2055
MlyI GAGTC 2 cut(s) 1051, 1505
Mph1103I ATGCAT 1 cut(s) 364
MroNI GCCGGC 1 cut(s) 823
MroXI GAANNNNTTC 1 cut(s) 1281
MseI TTAA 8 cut(s) 732, 791, 1191, 1305, 1338, 1925, 2058, 2079
MslI CAYNNNNRTG 1 cut(s) 1859
MspA1I CMGCKG 4 cut(s) 201, 710, 2195, 2315
MspI CCGG 4 cut(s) 246, 797, 824, 829
MspR9I CCNGG 6 cut(s) 246, 266, 829, 836, 1106, 1176
Mva1269I GAATGC 2 cut(s) 2110, 2314
MvaI CCWGG 4 cut(s) 266, 836, 1106, 1176
MvnI CGCG 4 cut(s) 71, 209, 1288, 1350
NaeI GCCGGC 1 cut(s) 825
NciI CCSGG 2 cut(s) 246, 829
NcoI CCATGG 1 cut(s) 2372
NdeII GATC 8 cut(s) 445, 577, 831, 1108, 1327, 2065, 2163, 2368
NgoMIV GCCGGC 1 cut(s) 823
NlaIII CATG 8 cut(s) 28, 888, 1387, 1420, 1609, 1981, 2035, 2376
NlaIV GGNNCC 6 cut(s) 6, 131, 523, 833, 1110, 1185
NmeAIII GCCGAG 3 cut(s) 244, 259, 295
NmuCI GTSAC 3 cut(s) 388, 463, 1811
NruI TCGCGA 1 cut(s) 1350
NsiI ATGCAT 1 cut(s) 364
NspI RCATGY 1 cut(s) 1609
OliI CACNNNNGTG 1 cut(s) 1859
PagI TCATGA 2 cut(s) 1383, 2031
PctI GAATGC 2 cut(s) 2110, 2314
PdiI GCCGGC 1 cut(s) 825
PdmI GAANNNNTTC 1 cut(s) 1281
PfeI GAWTC 9 cut(s) 255, 413, 1319, 1354, 1380, 1420, 1750, 2175, 2297
PflMI CCANNNNNTGG 1 cut(s) 455
PfoI TCCNGGA 2 cut(s) 1104, 1174
PkrI GCNGC 9 cut(s) 120, 152, 203, 211, 706, 709, 1881, 2095, 2314
PleI GAGTC 2 cut(s) 1050, 1505
PpsI GAGTC 2 cut(s) 1050, 1505
Ppu21I YACGTR 1 cut(s) 1240
PpuMI RGGWCCY 1 cut(s) 2083
PshAI GACNNNNGTC 1 cut(s) 190
Psp5II RGGWCCY 1 cut(s) 2083
Psp6I CCWGG 4 cut(s) 264, 834, 1104, 1174
PspFI CCCAGC 2 cut(s) 83, 2191
PspGI CCWGG 4 cut(s) 264, 834, 1104, 1174
PspN4I GGNNCC 6 cut(s) 6, 131, 523, 833, 1110, 1185
PspPI GGNCC 7 cut(s) 90, 826, 1184, 1690, 2002, 2083, 2233
PspPPI RGGWCCY 1 cut(s) 2083
PsrI GAACNNNNNNTAC 2 cut(s) 763, 795
PstNI CAGNNNCTG 1 cut(s) 710
PsuI RGATCY 3 cut(s) 577, 831, 1108
PvuII CAGCTG 2 cut(s) 710, 2195
RruI TCGCGA 1 cut(s) 1350
RsaI GTAC 4 cut(s) 263, 1238, 1944, 2284
RsaNI GTAC 4 cut(s) 262, 1237, 1943, 2283
RseI CAYNNNNRTG 1 cut(s) 1859
SaqAI TTAA 8 cut(s) 732, 791, 1191, 1305, 1338, 1925, 2058, 2079
SatI GCNGC 9 cut(s) 119, 151, 202, 210, 705, 708, 1880, 2094, 2313
Sau3AI GATC 8 cut(s) 445, 577, 831, 1108, 1327, 2065, 2163, 2368
Sau96I GGNCC 7 cut(s) 90, 826, 1184, 1690, 2002, 2083, 2233
SchI GAGTC 2 cut(s) 1051, 1505
ScrFI CCNGG 6 cut(s) 246, 266, 829, 836, 1106, 1176
SduI GDGCHC 2 cut(s) 132, 1672
SfaNI GCATC 8 cut(s) 229, 349, 444, 1026, 1649, 2113, 2293, 2354
SfcI CTRYAG 3 cut(s) 425, 906, 2136
SinI GGWCC 2 cut(s) 1690, 2083
SmiMI CAYNNNNRTG 1 cut(s) 1859
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
Sse9I AATT 7 cut(s) 608, 812, 850, 1007, 1076, 1371, 2055
SspI AATATT 1 cut(s) 2049
SspMI CTAG 2 cut(s) 354, 900
StyD4I CCNGG 6 cut(s) 244, 264, 827, 834, 1104, 1174
StyI CCWWGG 3 cut(s) 674, 2005, 2372
TaaI ACNGT 8 cut(s) 463, 515, 716, 861, 907, 1281, 1649, 2282
TaiI ACGT 3 cut(s) 769, 1234, 1242
TaqI TCGA 3 cut(s) 258, 1369, 1916
TasI AATT 7 cut(s) 608, 812, 850, 1007, 1076, 1371, 2055
TatI WGTACW 1 cut(s) 1942
TauI GCSGC 4 cut(s) 121, 153, 212, 2315
TfiI GAWTC 9 cut(s) 255, 413, 1319, 1354, 1380, 1420, 1750, 2175, 2297
Tru1I TTAA 8 cut(s) 732, 791, 1191, 1305, 1338, 1925, 2058, 2079
Tru9I TTAA 8 cut(s) 732, 791, 1191, 1305, 1338, 1925, 2058, 2079
TscAI CASTG 4 cut(s) 1141, 2020, 2125, 2176
TseFI GTSAC 3 cut(s) 388, 463, 1811
TseI GCWGC 5 cut(s) 201, 704, 707, 1879, 2093
Tsp45I GTSAC 3 cut(s) 388, 463, 1811
TspDTI ATGAA 8 cut(s) 290, 825, 1032, 1212, 1477, 1749, 2006, 2020
TspGWI ACGGA 1 cut(s) 184
TspRI CASTG 4 cut(s) 1141, 2020, 2125, 2176
Van91I CCANNNNNTGG 1 cut(s) 455
VpaK11BI GGWCC 2 cut(s) 1690, 2083
XapI RAATTY 2 cut(s) 812, 1076
XceI RCATGY 1 cut(s) 1609
XmiI GTMKAC 2 cut(s) 1651, 1782
XmnI GAANNNNTTC 1 cut(s) 1281
XspI CTAG 2 cut(s) 354, 900
Zsp2I ATGCAT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.