Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0000994

Physical Location & Seq

Genomic Coordinates Reverse (-)

8519352 .. 8521735

Gene Structure 2384 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1119200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 708 bp

ATGGCAGACGACAGACCGGAGAAGATAATCCTCAAAACCCCAGCACTTCTCAAGTACATCTTCGAAACAAGCTGCTTTCCAAGAGAACACGAGCAACTGAAGCAACTAAGGGAGGCCACCATCGAGAAATATGCTGTCTGGAGTCTTATGAATGTGCCTGTAGACGAAGGTCTGCTTGTATCGATGCTTCTGCAGGTGATGAATGCTAAGAAGACGTTGGAGCTCGGTGTCTTCACTGGTTATTCTCTTCTCACTACTGCTCTTGCACTGCCTGCTGATGGAAAAATAACAGCAATCGATGTAGATAAAGAAGCCTATGAGGTTGGACTACCATTTATTCAGAAGGTTGGGGTGGAACATAAGATTAACTTCTGCCAGTCAGATGCCTTCACGGTCCTAAATGATCTCATTACCAAAGGGGAGGAAGAAGGGAGCTTTGATTTTGCATTTGTGGATGCCGACAAGGACTCGTACATGGAATATCACAAGCAGCTGATGAAGCTTGTGAAGGTTGGAGGAATCATAGCTTATGATAACACGTTGTGGTTTGGGACAGTGGCGGAGCCTGAGGAGAGGAGAATGTGCAAGAATTTGCAAGGCAGAAAACATTTGCTGGAACTCAACAGCTTTCTCGCCGCGGATGACCCAGTTGAGCTAGCCGTTGTTTCCACCGGAGATGGACTCACCCTCTGCGGGCGTCTCTGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0009058 GO:0009718 GO:0009812 GO:0009813 GO:0016137 GO:0016138 GO:0016740 GO:0016741 GO:0032259 GO:0033485 GO:0033486 GO:0042440 GO:0043167 GO:0043169 GO:0043473 GO:0044424 GO:0044464 GO:0046148 GO:0046283 GO:0046872 GO:0071704 GO:1901038 GO:1901135 GO:1901137 GO:1901576 GO:1901657 GO:1901659 GO:1901804 GO:1901806

KEGG Pathways

Metabolic & Signaling

ko00360 ko00940 ko00941 ko00944 ko00945 ko01100 ko01110 map00360 map00940 map00941 map00944 map00945 map01100 map01110

Pfam Domains

Protein Families

Methyltransf_3

Protein Analysis

236

Amino Acids

26.3

Weight (kDa)

4.9

Isoelectric Point (pI)

34.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_3	PF01596	26 - 233	2.1e-78	O-methyltransferase
Methyltransf_24	PF13578	73 - 179	7.7e-10	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000994)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24735 AT1G24735 AT1G24735 AT1G67980 AT1G67980 AT1G67990
fragaria_vesca	FvH4_1g06880 FvH4_4g23870 FvH4_4g23870 FvH4_4g23880 FvH4_4g23880 FvH4_5g35170 FvH4_5g35170
malus_domestica	MD02G1073200.v1.1 MD02G1230800.v1.1 MD13G1117900.v1.1 MD16G1119200.v1.1 MD16G1119300.v1.1
prunus_persica	Prupe.1G227100_v2.0.a1 Prupe.1G227100_v2.0.a1 Prupe.1G227100_v2.0.a1 Prupe.1G227100_v2.0.a1 Prupe.2G107200_v2.0.a1 Prupe.2G107300_v2.0.a1
pyrus_communis	pycom02g19990 pycom13g10240 pycom16g10120
rosa_chinensis	RchiOBHm_Chr0c11g0499431 RchiOBHm_Chr4g0430461 RchiOBHm_Chr4g0430471 RchiOBHm_Chr4g0430481 RchiOBHm_Chr7g0236261
rosa_laevigata	RLG00000001094 RLG00000001944 RLG00000006974 RLG00000006975
rosa_multiflora	Rmu_co8207790.1_g000001 Rmu_co8303449.1_g000001 Rmu_sc0000131.1_g000035 Rmu_sc0000131.1_g000038 Rmu_sc0017358.1_g000001 Rmu_sc0017359.1_g000001
rosa_roxburghii	Rroxscaffold_2G00147630 Rroxscaffold_2G00147640 Rroxscaffold_5G00372230 Rroxscaffold_5G00372250
rosa_rugosa	Rorug04G0243400 Rorug04G0243500 Rorug04G0243500 Rorug04G0243600
rosa_samantha	Rh4AG299800 Rh4BG306800 Rh4BG306900 Rh4CG322700 Rh4CG323000 Rh4DG303800 Rh4DG304000 Rh4DG304100 Rh7BG421700
rosa_wichuraiana	Rw4G026020 Rw4G026030 Rw7G037400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	184
Acc36I	ACCTGC	1 cut(s)	184
AccI	GTMKAC	1 cut(s)	162
AccII	CGCG	1 cut(s)	638
AciI	CCGC	4 cut(s)	560, 636, 638, 693
AcsI	RAATTY	1 cut(s)	589
AcuI	CTGAAG	1 cut(s)	119
AcyI	GRCGYC	1 cut(s)	697
AdeI	CACNNNGTG	1 cut(s)	543
AfaI	GTAC	2 cut(s)	56, 473
AfiI	CCNNNNNNNGG	2 cut(s)	278, 693
AflIII	ACRYGT	1 cut(s)	537
AluBI	AGCT	8 cut(s)	72, 223, 435, 493, 502, 527, 627, 655
AluI	AGCT	8 cut(s)	72, 223, 435, 493, 502, 527, 627, 655
Alw21I	GWGCWC	1 cut(s)	225
Alw26I	GTCTC	1 cut(s)	704
AoxI	GGCC	1 cut(s)	114
ApeKI	GCWGC	2 cut(s)	72, 490
ApoI	RAATTY	1 cut(s)	589
AspS9I	GGNCC	1 cut(s)	394
AsuHPI	GGTGA	2 cut(s)	208, 676
AsuII	TTCGAA	1 cut(s)	63
AsuNHI	GCTAGC	1 cut(s)	655
AvaII	GGWCC	1 cut(s)	394
AxyI	CCTNAGG	1 cut(s)	567
BanII	GRGCYC	1 cut(s)	225
BarI	GAAGNNNNNNTAC	2 cut(s)	171, 203
BauI	CACGAG	1 cut(s)	89
BbsI	GAAGAC	2 cut(s)	218, 223
Bbv12I	GWGCWC	1 cut(s)	225
BbvI	GCAGC	2 cut(s)	59, 502
BccI	CCATC	3 cut(s)	128, 272, 671
BceAI	ACGGC	1 cut(s)	644
BcgI	CGANNNNNNTGC	4 cut(s)	113, 147, 172, 206
BcoDI	GTCTC	1 cut(s)	704
BfaI	CTAG	1 cut(s)	656
BfmI	CTRYAG	2 cut(s)	159, 191
BfuAI	ACCTGC	1 cut(s)	184
BisI	GCNGC	3 cut(s)	73, 491, 636
BlsI	GCNGC	3 cut(s)	74, 492, 637
Bme18I	GGWCC	1 cut(s)	394
BmgT120I	GGNCC	1 cut(s)	394
BmiI	GGNNCC	1 cut(s)	564
BmrI	ACTGGG	1 cut(s)	641
BmsI	GCATC	3 cut(s)	174, 373, 445
BmtI	GCTAGC	1 cut(s)	659
BmuI	ACTGGG	1 cut(s)	641
BoxI	GACNNNNGTC	1 cut(s)	168
BpiI	GAAGAC	2 cut(s)	218, 223
BplI	GAGNNNNNCTC	2 cut(s)	666, 698
BpmI	CTGGAG	1 cut(s)	160
Bpu14I	TTCGAA	1 cut(s)	63
BpuEI	CTTGAG	1 cut(s)	35
Bsa29I	ATCGAT	2 cut(s)	182, 297
BsaHI	GRCGYC	1 cut(s)	697
BsaJI	CCNNGG	1 cut(s)	636
BsaWI	WCCGGW	2 cut(s)	16, 671
Bsc4I	CCNNNNNNNGG	2 cut(s)	278, 693
Bse1I	ACTGG	3 cut(s)	241, 376, 647
Bse21I	CCTNAGG	1 cut(s)	567
BseCI	ATCGAT	2 cut(s)	182, 297
BseDI	CCNNGG	1 cut(s)	636
BseGI	GGATG	2 cut(s)	460, 646
BseLI	CCNNNNNNNGG	2 cut(s)	278, 693
BseMII	CTCAG	1 cut(s)	558
BseNI	ACTGG	3 cut(s)	241, 376, 647
BseRI	GAGGAG	2 cut(s)	584, 589
BseXI	GCAGC	2 cut(s)	59, 502
BseYI	CCCAGC	1 cut(s)	40
Bsh1236I	CGCG	1 cut(s)	638
BshFI	GGCC	1 cut(s)	116
BshVI	ATCGAT	2 cut(s)	182, 297
BsiHKAI	GWGCWC	1 cut(s)	225
BsiSI	CCGG	2 cut(s)	17, 672
BslFI	GGGAC	1 cut(s)	565
BslI	CCNNNNNNNGG	2 cut(s)	278, 693
BsmAI	GTCTC	1 cut(s)	704
BsmBI	CGTCTC	1 cut(s)	704
BsmFI	GGGAC	1 cut(s)	565
BsmI	GAATGC	1 cut(s)	208
BsnI	GGCC	1 cut(s)	116
Bsp119I	TTCGAA	1 cut(s)	63
Bsp1286I	GDGCHC	1 cut(s)	225
Bsp143I	GATC	1 cut(s)	403
BspACI	CCGC	4 cut(s)	560, 636, 638, 693
BspANI	GGCC	1 cut(s)	116
BspCNI	CTCAG	1 cut(s)	559
BspDI	ATCGAT	2 cut(s)	182, 297
BspFNI	CGCG	1 cut(s)	638
BspLI	GGNNCC	1 cut(s)	564
BspMAI	CTGCAG	1 cut(s)	195
BspMI	ACCTGC	1 cut(s)	184
BspOI	GCTAGC	1 cut(s)	659
BspT104I	TTCGAA	1 cut(s)	63
BsrI	ACTGG	3 cut(s)	241, 376, 647
BssECI	CCNNGG	1 cut(s)	636
BssMI	GATC	1 cut(s)	403
BssNI	GRCGYC	1 cut(s)	697
BssSI	CACGAG	1 cut(s)	89
Bst2BI	CACGAG	1 cut(s)	89
Bst4CI	ACNGT	2 cut(s)	394, 556
Bst6I	CTCTTC	1 cut(s)	252
BstACI	GRCGYC	1 cut(s)	697
BstAPI	GCANNNNNTGC	1 cut(s)	272
BstBI	TTCGAA	1 cut(s)	63
BstC8I	GCNNGC	3 cut(s)	273, 657, 695
BstDEI	CTNAG	3 cut(s)	107, 207, 567
BstDSI	CCRYGG	1 cut(s)	636
BstF5I	GGATG	2 cut(s)	460, 646
BstFNI	CGCG	1 cut(s)	638
BstKTI	GATC	1 cut(s)	406
BstMAI	GTCTC	1 cut(s)	704
BstMBI	GATC	1 cut(s)	403
BstMWI	GCNNNNNNNGC	3 cut(s)	100, 272, 499
BstPAI	GACNNNNGTC	1 cut(s)	168
BstSFI	CTRYAG	2 cut(s)	159, 191
BstUI	CGCG	1 cut(s)	638
BstV1I	GCAGC	2 cut(s)	59, 502
BstV2I	GAAGAC	2 cut(s)	218, 223
Bsu15I	ATCGAT	2 cut(s)	182, 297
Bsu36I	CCTNAGG	1 cut(s)	567
BsuRI	GGCC	1 cut(s)	116
BsuTUI	ATCGAT	2 cut(s)	182, 297
BtgI	CCRYGG	1 cut(s)	636
BtsCI	GGATG	2 cut(s)	460, 646
BtsI	GCAGTG	1 cut(s)	266
BtsIMutI	CAGTG	3 cut(s)	234, 266, 561
BveI	ACCTGC	1 cut(s)	184
Cac8I	GCNNGC	3 cut(s)	273, 657, 695
Cfr13I	GGNCC	1 cut(s)	394
Cfr42I	CCGCGG	1 cut(s)	639
ClaI	ATCGAT	2 cut(s)	182, 297
CseI	GACGC	1 cut(s)	686
Csp6I	GTAC	2 cut(s)	55, 472
CviAII	CATG	1 cut(s)	475
CviQI	GTAC	2 cut(s)	55, 472
DdeI	CTNAG	3 cut(s)	107, 207, 567
DpnI	GATC	1 cut(s)	405
DpnII	GATC	1 cut(s)	403
DraIII	CACNNNGTG	1 cut(s)	543
Eam1104I	CTCTTC	1 cut(s)	252
EarI	CTCTTC	1 cut(s)	252
EciI	GGCGGA	1 cut(s)	575
Ecl136II	GAGCTC	1 cut(s)	223
Eco24I	GRGCYC	1 cut(s)	225
Eco47I	GGWCC	1 cut(s)	394
Eco53kI	GAGCTC	1 cut(s)	223
Eco57I	CTGAAG	1 cut(s)	119
Eco81I	CCTNAGG	1 cut(s)	567
EcoICRI	GAGCTC	1 cut(s)	223
EcoT38I	GRGCYC	1 cut(s)	225
Esp3I	CGTCTC	1 cut(s)	704
FaeI	CATG	1 cut(s)	478
FaiI	YATR	7 cut(s)	132, 149, 318, 360, 476, 524, 531
FalI	AAGNNNNNCTT	6 cut(s)	44, 76, 159, 191, 353, 385
FaqI	GGGAC	1 cut(s)	565
FatI	CATG	1 cut(s)	474
FauI	CCCGC	1 cut(s)	686
FblI	GTMKAC	1 cut(s)	162
Fnu4HI	GCNGC	3 cut(s)	73, 491, 636
FokI	GGATG	2 cut(s)	467, 653
FriOI	GRGCYC	1 cut(s)	225
Fsp4HI	GCNGC	3 cut(s)	73, 491, 636
FspBI	CTAG	1 cut(s)	656
GluI	GCNGC	3 cut(s)	73, 491, 636
GsaI	CCCAGC	1 cut(s)	44
GsuI	CTGGAG	1 cut(s)	160
HaeIII	GGCC	1 cut(s)	116
HapII	CCGG	2 cut(s)	17, 672
HgaI	GACGC	1 cut(s)	686
Hin1I	GRCGYC	1 cut(s)	697
Hin1II	CATG	1 cut(s)	478
HindIII	AAGCTT	1 cut(s)	500
HinfI	GANTC	4 cut(s)	142, 467, 519, 681
HpaII	CCGG	2 cut(s)	17, 672
HphI	GGTGA	2 cut(s)	208, 676
Hpy166II	GTNNAC	1 cut(s)	163
Hpy188I	TCNGA	2 cut(s)	342, 382
Hpy188III	TCNNGA	2 cut(s)	124, 139
Hpy8I	GTNNAC	1 cut(s)	163
HpyAV	CCTTC	5 cut(s)	161, 337, 397, 422, 502
HpyCH4III	ACNGT	2 cut(s)	394, 556
HpyCH4IV	ACGT	2 cut(s)	215, 539
HpyCH4V	TGCA	5 cut(s)	193, 266, 446, 585, 595
HpyF10VI	GCNNNNNNNGC	3 cut(s)	100, 272, 499
HpyF3I	CTNAG	3 cut(s)	107, 207, 567
HpySE526I	ACGT	2 cut(s)	215, 539
Hsp92I	GRCGYC	1 cut(s)	697
Hsp92II	CATG	1 cut(s)	478
KspI	CCGCGG	1 cut(s)	639
Kzo9I	GATC	1 cut(s)	403
LmnI	GCTCC	3 cut(s)	220, 432, 562
Lsp1109I	GCAGC	2 cut(s)	59, 502
LweI	GCATC	3 cut(s)	174, 373, 445
MaeI	CTAG	1 cut(s)	656
MaeII	ACGT	2 cut(s)	215, 539
MalI	GATC	1 cut(s)	405
MboI	GATC	1 cut(s)	403
MboII	GAAGA	6 cut(s)	34, 52, 223, 223, 239, 437
MhlI	GDGCHC	1 cut(s)	225
MluCI	AATT	1 cut(s)	589
MlyI	GAGTC	3 cut(s)	151, 461, 675
MmeI	TCCRAC	3 cut(s)	198, 304, 493
MnlI	CCTC	8 cut(s)	41, 106, 313, 415, 509, 562, 567, 698
MseI	TTAA	1 cut(s)	366
MspA1I	CMGCKG	2 cut(s)	493, 638
MspI	CCGG	2 cut(s)	17, 672
Mva1269I	GAATGC	1 cut(s)	208
MvnI	CGCG	1 cut(s)	638
MwoI	GCNNNNNNNGC	3 cut(s)	100, 272, 499
NdeII	GATC	1 cut(s)	403
NheI	GCTAGC	1 cut(s)	655
NlaIII	CATG	1 cut(s)	478
NlaIV	GGNNCC	1 cut(s)	564
NspV	TTCGAA	1 cut(s)	63
PaqCI	CACCTGC	1 cut(s)	184
PctI	GAATGC	1 cut(s)	208
PfeI	GAWTC	1 cut(s)	519
PkrI	GCNGC	3 cut(s)	74, 492, 637
PleI	GAGTC	3 cut(s)	150, 461, 675
PpsI	GAGTC	3 cut(s)	150, 461, 675
PshAI	GACNNNNGTC	1 cut(s)	168
Psp124BI	GAGCTC	1 cut(s)	225
PspFI	CCCAGC	1 cut(s)	40
PspN4I	GGNNCC	1 cut(s)	564
PspPI	GGNCC	1 cut(s)	394
PstI	CTGCAG	1 cut(s)	195
PvuII	CAGCTG	1 cut(s)	493
RsaI	GTAC	2 cut(s)	56, 473
RsaNI	GTAC	2 cut(s)	55, 472
SacI	GAGCTC	1 cut(s)	225
SacII	CCGCGG	1 cut(s)	639
SaqAI	TTAA	1 cut(s)	366
SatI	GCNGC	3 cut(s)	73, 491, 636
Sau3AI	GATC	1 cut(s)	403
Sau96I	GGNCC	1 cut(s)	394
SchI	GAGTC	3 cut(s)	151, 461, 675
SduI	GDGCHC	1 cut(s)	225
SfaNI	GCATC	3 cut(s)	174, 373, 445
SfcI	CTRYAG	2 cut(s)	159, 191
Sfr303I	CCGCGG	1 cut(s)	639
SfuI	TTCGAA	1 cut(s)	63
SgrBI	CCGCGG	1 cut(s)	639
SinI	GGWCC	1 cut(s)	394
SmlI	CTYRAG	1 cut(s)	50
SmoI	CTYRAG	1 cut(s)	50
Sse9I	AATT	1 cut(s)	589
SsiI	CCGC	4 cut(s)	560, 636, 638, 693
SspMI	CTAG	1 cut(s)	656
SstI	GAGCTC	1 cut(s)	225
TaaI	ACNGT	2 cut(s)	394, 556
TaiI	ACGT	2 cut(s)	218, 542
TaqI	TCGA	4 cut(s)	63, 123, 182, 297
TasI	AATT	1 cut(s)	589
TatI	WGTACW	1 cut(s)	54
TauI	GCSGC	1 cut(s)	638
TfiI	GAWTC	1 cut(s)	519
Tru1I	TTAA	1 cut(s)	366
Tru9I	TTAA	1 cut(s)	366
TscAI	CASTG	3 cut(s)	241, 273, 561
TseI	GCWGC	2 cut(s)	72, 490
TspDTI	ATGAA	3 cut(s)	164, 215, 512
TspRI	CASTG	3 cut(s)	241, 273, 561
VpaK11BI	GGWCC	1 cut(s)	394
XapI	RAATTY	1 cut(s)	589
XmiI	GTMKAC	1 cut(s)	162
XspI	CTAG	1 cut(s)	656

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.