Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001139

Physical Location & Seq

Genomic Coordinates Forward (+)

16955726 .. 16969515

Gene Structure 13790 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1191900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2022 bp

ATGGCGGCTTCAACTATGAAACTAACGCTCTTCTTACTCCCTTTGTTCTTCACCTTCTCCCTAATAATCTCAAGCCACCACCCACTAGATCCTTTAACCCCATCTGAATTCAAAAAGGTCCGAACCATAGTAAAAAAATCATACCCATCTGGCCTCAAACACAACCTAACCTTTCAATACATAGGTTTAGACGAGCCAGACAAACCAGTCCTCCAATCATGGCTTTCATCAAAAACCAAATCAAACCCCCCTCCTCGACGAGCCTTTGTCATCGCACGCCTCCACAAAGAAACCCACGAGCTCGTGGTCGATTTATCCAATCGTTACATAATTTCCGATAAAGTTTACGGTGGAAATGATGGTTACCCTATGCTCACCAACGACGAACAAACAGCGGCAAGTGCACTTCCGTTTTCCTATCAACCATTTATCGATTCGATTAAGAAGAGGGAGCTTAACTTGTCCGAGGTTGTTTGTACCACGTTTACAGTTGGGTGGTTTGGGGAGAAGCATAGTAGAAGAGCAATTAAAATTCTGTGTTTTTATAAAGAGGGCACGGCTAATGTGTACATGAGGCCCTTGGAGGGAGTATCTTTGTTGGTTGATCTTGATGAAATGAAAATAGTGGAGTATTATGACAGGTCTAGGCTTCCGATGCCGAAGGCTGAAGGAACGGAGTATCGGCTGTCAAAGCAGAAGCCGCCATTTGGTCCTCCGCTGAATGGTTTTGTCATCGAGCAACCGAACGGACCCGGGTTCAAAATCGACGGGCACAAAATAAGCTGGGCCAATTGGGATTTCCATTTGGGATTTGATGTTCGAGCTGGTCCAATAATTTCGTTGGCATCAATCTACGATCTAGAAAAGGCTACATATCGTCAAGTATTATACAGAGCATTCGTATCCGAGCTTTTTGTGCCGTACATGGACCCAACTGAAGAATGGTACTACAAGACCTTCTTTGATGCCGGTGAATTTGGTTTTGGTCAACTGGCCATGCCACTTGAGCCCTTGACTGATTGCCCTGCCAATGCTGAGTTCTTGGATGCATACTATGCCGGGGAGGATGGTACTCCTGTTAAAATATCTAATGCTTTATGCATATTTGAGCAACAGTCTGGGAGTGTCATGTGGCGTCACACTGAATCAGCTATTCCTGGAGAACTGATAACGGAGGTTAGGCCAGAACTAAGCTTGATAGTGAGGATGGTCGCAACTGTTGGCAACTACGATTACATACTCGACTGGGAATTTAAGCCAAGCGGCTCCATCAAAATTGGGGTTGGCTTAACAGGAATACTAGAAGTGAAGGCTGCAGATTACACTCACACTAGTCAAATAAAGGAGGATGCTTATGGTACATTGCTAGCAGATTATACAATTGGGGTGTACCACGACCATTTCTTGACCTATTACCTGGATCTTGATGTGGATGGTGAAGCCAACTCCTTTATTAAAAACAATTTGGTGACCAAAAGAGTGACAAACAAGAACTCACCTAGAAAGAGCTACTGGACAGTTGAAAGTGAGACAGCAAAAACCGAATTGGATGCAAAAATTCAGTTGGGTTTGAAGGCATCGGAGCTGGTTGTGGTAAACCCTAACAAGAAGACCAAACCTGGAAACAGCATTGGTTATCGTCTAATTCCAGGGTCAATATCACAAGCACTTTTAAGCGACGATGATTACTCACAAATCCGAGGTGCATTTACCAATTACAATGTATGGGTCACACCTTATAACAAGTCTGAAAAATGGGCTGGAGGACTATATGCTGATCGGAGCCATGGAGAAGACACACTGGCTACTTGGAGCCTCAGGAACCGAGAGATTGAAAATAAGGACATAGTGTTATGGTACACCGTGGGGTTTCATCATGTTCCTTGCCAAGAAGATTTTCCAATTATGCCAACATTAAGCGGTGGATTTGAGCTTCGGCCTACAAATTTCTTTGAGAGCAATCCTGTGCTTAAAACGAAACCTCCAAGTTCTAAGCATGTTAACTCCACAATACAACCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009987 GO:0010035 GO:0016491 GO:0016638 GO:0016641 GO:0042221 GO:0050896 GO:0051716 GO:0052597 GO:0055114 GO:0070887 GO:0071241 GO:0097184 GO:0097185 GO:1901698 GO:1901699

KEGG Pathways

Metabolic & Signaling

ko00260 ko00350 ko00360 ko00410 ko00950 ko00960 ko01100 ko01110 map00260 map00350 map00360 map00410 map00950 map00960 map01100 map01110

Pfam Domains

Protein Families

Cu_amine_oxid Cu_amine_oxidN2 Cu_amine_oxidN3

Protein Analysis

674

Amino Acids

76.12

Weight (kDa)

6.02

Isoelectric Point (pI)

35.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cu_amine_oxidN2	PF02727	27 - 114	1.5e-18	Copper amine oxidase, N2 domain
Cu_amine_oxidN3	PF02728	123 - 217	3.6e-23	Copper amine oxidase, N3 domain
Cu_amine_oxid	PF01179	245 - 657	3.3e-141	Copper amine oxidase, enzyme domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001139)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31690 AT1G31710
fragaria_vesca	FvH4_2g30510 FvH4_4g15340 FvH4_4g15360 FvH4_4g15380
malus_domestica	MD16G1191900.v1.1 MD16G1192100.v1.1 MD16G1192300.v1.1
prunus_persica	Prupe.1G011900_v2.0.a1 Prupe.1G012000_v2.0.a1
pyrus_communis	pycom16g16160 pycom16g16180 pycom16g16190 pycom16g16200
rosa_chinensis	RchiOBHm_Chr2g0122101 RchiOBHm_Chr4g0416421 RchiOBHm_Chr4g0416431 RchiOBHm_Chr4g0416451 RchiOBHm_Chr4g0416461 RchiOBHm_Chr4g0416501
rosa_laevigata	RLG00000007978 RLG00000007979 RLG00000007982 RLG00000007983
rosa_multiflora	Rmu_co8318835.1_g000001 Rmu_sc0003722.1_g000010 Rmu_sc0003722.1_g000023
rosa_roxburghii	Rroxscaffold_2G00090210 Rroxscaffold_5G00360250 Rroxscaffold_5G00360280 Rroxscaffold_5G00360360 Rroxscaffold_5G00360370
rosa_rugosa	Rorug04G0141800 Rorug04G0141900 Rorug04G0142200
rosa_samantha	Rh4AG204100 Rh4AG204200 Rh4AG204300 Rh4AG204600 Rh4BG201800 Rh4BG202200 Rh4BG202400 Rh4CG215300 Rh4CG215500 Rh4CG215800 Rh4DG201600 Rh4DG201700 Rh4DG201800 Rh5BG514400 Rh5CG538000 Rh5DG526800 Rh7AG414700 Rh7AG415300
rosa_wichuraiana	Rw4G017280 Rw4G017290 Rw4G017310 Rw5G045710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	546, 1740
AasI	GACNNNNNNGTC	1 cut(s)	206
AciI	CCGC	6 cut(s)	5, 395, 701, 716, 1263, 1920
AclWI	GGATC	2 cut(s)	83, 1428
AcoI	YGGCCR	1 cut(s)	993
AcsI	RAATTY	6 cut(s)	107, 531, 974, 1250, 1557, 1945
AcuI	CTGAAG	2 cut(s)	687, 957
AcyI	GRCGYC	1 cut(s)	1135
AfaI	GTAC	8 cut(s)	478, 569, 923, 947, 1072, 1360, 1391, 1859
AfiI	CCNNNNNNNGG	3 cut(s)	584, 707, 722
AgsI	TTSAA	7 cut(s)	12, 112, 176, 760, 1523, 1573, 1835
AhlI	ACTAGT	1 cut(s)	1331
AjnI	CCWGG	4 cut(s)	1156, 1416, 1618, 1648
AjuI	GAANNNNNNNTTGG	8 cut(s)	782, 814, 966, 998, 1022, 1054, 1881, 1913
AloI	GAACNNNNNNTCC	2 cut(s)	801, 833
Alw21I	GWGCWC	2 cut(s)	303, 406
Alw26I	GTCTC	1 cut(s)	1523
Alw44I	GTGCAC	1 cut(s)	402
AlwI	GGATC	2 cut(s)	83, 1428
Ama87I	CYCGRG	1 cut(s)	752
AoxI	GGCC	6 cut(s)	151, 575, 786, 993, 1181, 1937
ApaLI	GTGCAC	1 cut(s)	402
ApeKI	GCWGC	1 cut(s)	1313
ApoI	RAATTY	6 cut(s)	107, 531, 974, 1250, 1557, 1945
Asp700I	GAANNNNTTC	1 cut(s)	1896
AspS9I	GGNCC	7 cut(s)	118, 576, 710, 749, 786, 827, 928
AsuC2I	CCSGG	3 cut(s)	753, 754, 1060
AsuHPI	GGTGA	6 cut(s)	43, 367, 983, 1448, 1480, 1488
AsuNHI	GCTAGC	1 cut(s)	1366
AvaI	CYCGRG	1 cut(s)	752
AvaII	GGWCC	5 cut(s)	118, 710, 749, 827, 928
AxyI	CCTNAGG	1 cut(s)	1817
BaeGI	GKGCMC	3 cut(s)	406, 557, 774
BaeI	ACNNNNGTAYC	2 cut(s)	1062, 1095
BalI	TGGCCA	1 cut(s)	995
BanII	GRGCYC	2 cut(s)	303, 1011
BauI	CACGAG	2 cut(s)	296, 302
BbsI	GAAGAC	2 cut(s)	1616, 1800
Bbv12I	GWGCWC	2 cut(s)	303, 406
BbvI	GCAGC	1 cut(s)	1300
BccI	CCATC	7 cut(s)	109, 154, 353, 1061, 1201, 1277, 1427
BceAI	ACGGC	2 cut(s)	573, 904
BcgI	CGANNNNNNTGC	2 cut(s)	1532, 1566
BciT130I	CCWGG	4 cut(s)	1158, 1418, 1620, 1650
BciVI	GTATCC	1 cut(s)	913
BcnI	CCSGG	3 cut(s)	753, 754, 1060
BcoDI	GTCTC	1 cut(s)	1523
BcuI	ACTAGT	1 cut(s)	1331
BfaI	CTAG	7 cut(s)	86, 645, 860, 1301, 1332, 1367, 1500
BfmI	CTRYAG	1 cut(s)	1314
BfuI	GTATCC	1 cut(s)	913
BisI	GCNGC	5 cut(s)	6, 396, 701, 1264, 1314
BlsI	GCNGC	5 cut(s)	7, 397, 702, 1265, 1315
Bme1390I	CCNGG	7 cut(s)	753, 754, 1060, 1158, 1418, 1620, 1650
Bme18I	GGWCC	5 cut(s)	118, 710, 749, 827, 928
BmeT110I	CYCGRG	1 cut(s)	752
BmgT120I	GGNCC	7 cut(s)	118, 576, 710, 749, 786, 827, 928
BmiI	GGNNCC	6 cut(s)	751, 930, 1267, 1784, 1814, 1823
BmrFI	CCNGG	7 cut(s)	753, 754, 1060, 1158, 1418, 1620, 1650
BmrI	ACTGGG	1 cut(s)	1255
BmsI	GCATC	7 cut(s)	645, 854, 955, 1036, 1339, 1540, 1586
BmtI	GCTAGC	1 cut(s)	1370
BmuI	ACTGGG	1 cut(s)	1255
BpiI	GAAGAC	2 cut(s)	1616, 1800
BpmI	CTGGAG	2 cut(s)	1179, 1782
BpuEI	CTTGAG	2 cut(s)	55, 1025
BpuMI	CCSGG	3 cut(s)	753, 754, 1060
Bsa29I	ATCGAT	1 cut(s)	432
BsaHI	GRCGYC	1 cut(s)	1135
BsaJI	CCNNGG	8 cut(s)	465, 579, 752, 1059, 1649, 1699, 1786, 1863
BsaXI	ACNNNNNCTCC	4 cut(s)	195, 225, 1966, 1996
Bsc4I	CCNNNNNNNGG	3 cut(s)	584, 707, 722
Bse118I	RCCGGY	1 cut(s)	968
Bse1I	ACTGG	5 cut(s)	206, 996, 1250, 1517, 1806
Bse21I	CCTNAGG	1 cut(s)	1817
Bse3DI	GCAATG	1 cut(s)	1361
BseBI	CCWGG	4 cut(s)	1158, 1418, 1620, 1650
BseCI	ATCGAT	1 cut(s)	432
BseDI	CCNNGG	8 cut(s)	465, 579, 752, 1059, 1649, 1699, 1786, 1863
BseGI	GGATG	6 cut(s)	1051, 1072, 1212, 1354, 1438, 1555
BseLI	CCNNNNNNNGG	3 cut(s)	584, 707, 722
BseMI	GCAATG	1 cut(s)	1361
BseMII	CTCAG	2 cut(s)	1026, 1831
BseNI	ACTGG	5 cut(s)	206, 996, 1250, 1517, 1806
BseRI	GAGGAG	1 cut(s)	243
BseSI	GKGCMC	3 cut(s)	406, 557, 774
BseXI	GCAGC	1 cut(s)	1300
BseYI	CCCAGC	1 cut(s)	783
BshFI	GGCC	6 cut(s)	153, 577, 788, 995, 1183, 1939
BshVI	ATCGAT	1 cut(s)	432
BsiHKAI	GWGCWC	2 cut(s)	303, 406
BsiHKCI	CYCGRG	1 cut(s)	752
BsiSI	CCGG	3 cut(s)	753, 969, 1059
BslI	CCNNNNNNNGG	3 cut(s)	584, 707, 722
BsmAI	GTCTC	1 cut(s)	1523
BsmI	GAATGC	1 cut(s)	896
BsnI	GGCC	6 cut(s)	153, 577, 788, 995, 1183, 1939
BsoBI	CYCGRG	1 cut(s)	752
Bsp1286I	GDGCHC	5 cut(s)	303, 406, 557, 774, 1011
Bsp1407I	TGTACA	1 cut(s)	567
Bsp143I	GATC	5 cut(s)	88, 604, 856, 1420, 1777
Bsp19I	CCATGG	1 cut(s)	1786
BspACI	CCGC	6 cut(s)	5, 395, 701, 716, 1263, 1920
BspANI	GGCC	6 cut(s)	153, 577, 788, 995, 1183, 1939
BspCNI	CTCAG	2 cut(s)	1027, 1830
BspDI	ATCGAT	1 cut(s)	432
BspLI	GGNNCC	6 cut(s)	751, 930, 1267, 1784, 1814, 1823
BspMAI	CTGCAG	1 cut(s)	1318
BspOI	GCTAGC	1 cut(s)	1370
BspPI	GGATC	2 cut(s)	83, 1428
BspQI	GCTCTTC	2 cut(s)	35, 514
BsrDI	GCAATG	1 cut(s)	1361
BsrFI	RCCGGY	1 cut(s)	968
BsrGI	TGTACA	1 cut(s)	567
BsrI	ACTGG	5 cut(s)	206, 996, 1250, 1517, 1806
BssAI	RCCGGY	1 cut(s)	968
BssECI	CCNNGG	8 cut(s)	465, 579, 752, 1059, 1649, 1699, 1786, 1863
BssMI	GATC	5 cut(s)	88, 604, 856, 1420, 1777
BssNI	GRCGYC	1 cut(s)	1135
BssSI	CACGAG	2 cut(s)	296, 302
BssT1I	CCWWGG	2 cut(s)	579, 1786
Bst2BI	CACGAG	2 cut(s)	296, 302
Bst2UI	CCWGG	4 cut(s)	1158, 1418, 1620, 1650
Bst4CI	ACNGT	6 cut(s)	350, 490, 1116, 1219, 1519, 1864
Bst6I	CTCTTC	3 cut(s)	35, 440, 514
BstACI	GRCGYC	1 cut(s)	1135
BstAPI	GCANNNNNTGC	1 cut(s)	1055
BstAUI	TGTACA	1 cut(s)	567
BstC8I	GCNNGC	2 cut(s)	277, 1368
BstDEI	CTNAG	4 cut(s)	1035, 1190, 1817, 1992
BstDSI	CCRYGG	2 cut(s)	1786, 1863
BstEII	GGTNACC	2 cut(s)	362, 1468
BstF5I	GGATG	6 cut(s)	1051, 1072, 1212, 1354, 1438, 1555
BstKTI	GATC	5 cut(s)	91, 607, 859, 1423, 1780
BstMAI	GTCTC	1 cut(s)	1523
BstMBI	GATC	5 cut(s)	88, 604, 856, 1420, 1777
BstMWI	GCNNNNNNNGC	7 cut(s)	401, 655, 691, 700, 916, 1006, 1055
BstNI	CCWGG	4 cut(s)	1158, 1418, 1620, 1650
BstNSI	RCATGY	1 cut(s)	2000
BstPI	GGTNACC	2 cut(s)	362, 1468
BstSCI	CCNGG	7 cut(s)	751, 752, 1058, 1156, 1416, 1618, 1648
BstSFI	CTRYAG	1 cut(s)	1314
BstSLI	GKGCMC	3 cut(s)	406, 557, 774
BstV1I	GCAGC	1 cut(s)	1300
BstV2I	GAAGAC	2 cut(s)	1616, 1800
BstX2I	RGATCY	2 cut(s)	88, 1420
BstYI	RGATCY	2 cut(s)	88, 1420
Bsu15I	ATCGAT	1 cut(s)	432
Bsu36I	CCTNAGG	1 cut(s)	1817
BsuI	GTATCC	1 cut(s)	913
BsuRI	GGCC	6 cut(s)	153, 577, 788, 995, 1183, 1939
BsuTUI	ATCGAT	1 cut(s)	432
BtgI	CCRYGG	2 cut(s)	1786, 1863
BtgZI	GCGATG	1 cut(s)	256
BtsCI	GGATG	6 cut(s)	1051, 1072, 1212, 1354, 1438, 1555
BtsIMutI	CAGTG	2 cut(s)	1140, 1799
Cac8I	GCNNGC	2 cut(s)	277, 1368
Cfr10I	RCCGGY	1 cut(s)	968
Cfr13I	GGNCC	7 cut(s)	118, 576, 710, 749, 786, 827, 928
Cfr9I	CCCGGG	1 cut(s)	752
ClaI	ATCGAT	1 cut(s)	432
CseI	GACGC	1 cut(s)	1124
Csp6I	GTAC	8 cut(s)	477, 568, 922, 946, 1071, 1359, 1390, 1858
CviAII	CATG	8 cut(s)	219, 571, 925, 997, 1129, 1787, 1877, 1997
CviQI	GTAC	8 cut(s)	477, 568, 922, 946, 1071, 1359, 1390, 1858
DdeI	CTNAG	4 cut(s)	1035, 1190, 1817, 1992
DpnI	GATC	5 cut(s)	90, 606, 858, 1422, 1779
DpnII	GATC	5 cut(s)	88, 604, 856, 1420, 1777
DrdI	GACNNNNNNGTC	1 cut(s)	206
DseDI	GACNNNNNNGTC	1 cut(s)	206
EaeI	YGGCCR	1 cut(s)	993
Eam1104I	CTCTTC	3 cut(s)	35, 440, 514
EarI	CTCTTC	3 cut(s)	35, 440, 514
Ecl136II	GAGCTC	1 cut(s)	301
Eco130I	CCWWGG	2 cut(s)	579, 1786
Eco24I	GRGCYC	2 cut(s)	303, 1011
Eco47I	GGWCC	5 cut(s)	118, 710, 749, 827, 928
Eco53kI	GAGCTC	1 cut(s)	301
Eco57I	CTGAAG	2 cut(s)	687, 957
Eco81I	CCTNAGG	1 cut(s)	1817
Eco88I	CYCGRG	1 cut(s)	752
Eco91I	GGTNACC	2 cut(s)	362, 1468
EcoICRI	GAGCTC	1 cut(s)	301
EcoO109I	RGGNCCY	1 cut(s)	576
EcoO65I	GGTNACC	2 cut(s)	362, 1468
EcoRI	GAATTC	1 cut(s)	107
EcoRII	CCWGG	4 cut(s)	1156, 1416, 1618, 1648
EcoT14I	CCWWGG	2 cut(s)	579, 1786
EcoT22I	ATGCAT	2 cut(s)	1051, 1103
EcoT38I	GRGCYC	2 cut(s)	303, 1011
ErhI	CCWWGG	2 cut(s)	579, 1786
FaeI	CATG	8 cut(s)	222, 574, 928, 1000, 1132, 1790, 1880, 2000
FalI	AAGNNNNNCTT	2 cut(s)	944, 976
FatI	CATG	8 cut(s)	218, 570, 924, 996, 1128, 1786, 1876, 1996
Fnu4HI	GCNGC	5 cut(s)	6, 396, 701, 1264, 1314
FokI	GGATG	6 cut(s)	1058, 1079, 1219, 1361, 1445, 1562
FriOI	GRGCYC	2 cut(s)	303, 1011
Fsp4HI	GCNGC	5 cut(s)	6, 396, 701, 1264, 1314
FspBI	CTAG	7 cut(s)	86, 645, 860, 1301, 1332, 1367, 1500
GluI	GCNGC	5 cut(s)	6, 396, 701, 1264, 1314
GsaI	CCCAGC	1 cut(s)	787
GsuI	CTGGAG	2 cut(s)	1179, 1782
HaeIII	GGCC	6 cut(s)	153, 577, 788, 995, 1183, 1939
HapII	CCGG	3 cut(s)	753, 969, 1059
HgaI	GACGC	1 cut(s)	1124
Hin1I	GRCGYC	1 cut(s)	1135
Hin1II	CATG	8 cut(s)	222, 574, 928, 1000, 1132, 1790, 1880, 2000
HincII	GTYRAC	2 cut(s)	989, 2002
HindII	GTYRAC	2 cut(s)	989, 2002
HindIII	AAGCTT	1 cut(s)	1192
HinfI	GANTC	2 cut(s)	434, 1145
HpaI	GTTAAC	1 cut(s)	2002
HpaII	CCGG	3 cut(s)	753, 969, 1059
HphI	GGTGA	6 cut(s)	43, 367, 983, 1448, 1480, 1488
Hpy166II	GTNNAC	9 cut(s)	346, 404, 486, 568, 989, 1390, 1597, 1860, 2002
Hpy188III	TCNNGA	5 cut(s)	608, 860, 1405, 1424, 1819
Hpy8I	GTNNAC	9 cut(s)	346, 404, 486, 568, 989, 1390, 1597, 1860, 2002
Hpy99I	CGWCG	4 cut(s)	261, 386, 770, 1682
HpyAV	CCTTC	6 cut(s)	64, 655, 662, 967, 1303, 1567
HpyCH4III	ACNGT	6 cut(s)	350, 490, 1116, 1219, 1519, 1864
HpyCH4IV	ACGT	1 cut(s)	482
HpyCH4V	TGCA	6 cut(s)	404, 1049, 1101, 1316, 1553, 1706
HpyF10VI	GCNNNNNNNGC	7 cut(s)	401, 655, 691, 700, 916, 1006, 1055
HpyF3I	CTNAG	4 cut(s)	1035, 1190, 1817, 1992
HpySE526I	ACGT	1 cut(s)	482
Hsp92I	GRCGYC	1 cut(s)	1135
Hsp92II	CATG	8 cut(s)	222, 574, 928, 1000, 1132, 1790, 1880, 2000
KspAI	GTTAAC	1 cut(s)	2002
Kzo9I	GATC	5 cut(s)	88, 604, 856, 1420, 1777
LguI	GCTCTTC	2 cut(s)	35, 514
LmnI	GCTCC	5 cut(s)	451, 1271, 1582, 1782, 1812
Lsp1109I	GCAGC	1 cut(s)	1300
LweI	GCATC	7 cut(s)	645, 854, 955, 1036, 1339, 1540, 1586
MaeI	CTAG	7 cut(s)	86, 645, 860, 1301, 1332, 1367, 1500
MaeII	ACGT	1 cut(s)	482
MaeIII	GTNAC	6 cut(s)	323, 362, 1136, 1468, 1480, 1729
MalI	GATC	5 cut(s)	90, 606, 858, 1422, 1779
MboI	GATC	5 cut(s)	88, 604, 856, 1420, 1777
MboII	GAAGA	8 cut(s)	22, 40, 457, 531, 950, 1621, 1805, 1904
MfeI	CAATTG	2 cut(s)	790, 1380
MflI	RGATCY	2 cut(s)	88, 1420
MhlI	GDGCHC	5 cut(s)	303, 406, 557, 774, 1011
MlsI	TGGCCA	1 cut(s)	995
MluNI	TGGCCA	1 cut(s)	995
Mox20I	TGGCCA	1 cut(s)	995
Mph1103I	ATGCAT	2 cut(s)	1051, 1103
MroXI	GAANNNNTTC	1 cut(s)	1896
MscI	TGGCCA	1 cut(s)	995
Msp20I	TGGCCA	1 cut(s)	995
MspA1I	CMGCKG	2 cut(s)	395, 718
MspI	CCGG	3 cut(s)	753, 969, 1059
MspR9I	CCNGG	7 cut(s)	753, 754, 1060, 1158, 1418, 1620, 1650
MunI	CAATTG	2 cut(s)	790, 1380
Mva1269I	GAATGC	1 cut(s)	896
MvaI	CCWGG	4 cut(s)	1158, 1418, 1620, 1650
MwoI	GCNNNNNNNGC	7 cut(s)	401, 655, 691, 700, 916, 1006, 1055
NciI	CCSGG	3 cut(s)	753, 754, 1060
NcoI	CCATGG	1 cut(s)	1786
NdeII	GATC	5 cut(s)	88, 604, 856, 1420, 1777
NheI	GCTAGC	1 cut(s)	1366
NlaIII	CATG	8 cut(s)	222, 574, 928, 1000, 1132, 1790, 1880, 2000
NlaIV	GGNNCC	6 cut(s)	751, 930, 1267, 1784, 1814, 1823
NmuCI	GTSAC	4 cut(s)	1136, 1468, 1480, 1729
NsiI	ATGCAT	2 cut(s)	1051, 1103
NspI	RCATGY	1 cut(s)	2000
PciSI	GCTCTTC	2 cut(s)	35, 514
PctI	GAATGC	1 cut(s)	896
PdmI	GAANNNNTTC	1 cut(s)	1896
PfeI	GAWTC	2 cut(s)	434, 1145
PfoI	TCCNGGA	1 cut(s)	1156
PkrI	GCNGC	5 cut(s)	7, 397, 702, 1265, 1315
PsiI	TTATAA	2 cut(s)	546, 1740
Psp124BI	GAGCTC	1 cut(s)	303
Psp6I	CCWGG	4 cut(s)	1156, 1416, 1618, 1648
PspEI	GGTNACC	2 cut(s)	362, 1468
PspFI	CCCAGC	1 cut(s)	783
PspGI	CCWGG	4 cut(s)	1156, 1416, 1618, 1648
PspN4I	GGNNCC	6 cut(s)	751, 930, 1267, 1784, 1814, 1823
PspPI	GGNCC	7 cut(s)	118, 576, 710, 749, 786, 827, 928
PstI	CTGCAG	1 cut(s)	1318
PsuI	RGATCY	2 cut(s)	88, 1420
RsaI	GTAC	8 cut(s)	478, 569, 923, 947, 1072, 1360, 1391, 1859
RsaNI	GTAC	8 cut(s)	477, 568, 922, 946, 1071, 1359, 1390, 1858
SacI	GAGCTC	1 cut(s)	303
SapI	GCTCTTC	2 cut(s)	35, 514
SatI	GCNGC	5 cut(s)	6, 396, 701, 1264, 1314
Sau3AI	GATC	5 cut(s)	88, 604, 856, 1420, 1777
Sau96I	GGNCC	7 cut(s)	118, 576, 710, 749, 786, 827, 928
ScrFI	CCNGG	7 cut(s)	753, 754, 1060, 1158, 1418, 1620, 1650
SduI	GDGCHC	5 cut(s)	303, 406, 557, 774, 1011
SfaNI	GCATC	7 cut(s)	645, 854, 955, 1036, 1339, 1540, 1586
SfcI	CTRYAG	1 cut(s)	1314
SinI	GGWCC	5 cut(s)	118, 710, 749, 827, 928
SmaI	CCCGGG	1 cut(s)	754
SmlI	CTYRAG	2 cut(s)	70, 1004
SmoI	CTYRAG	2 cut(s)	70, 1004
SpeI	ACTAGT	1 cut(s)	1331
SsiI	CCGC	6 cut(s)	5, 395, 701, 716, 1263, 1920
SspMI	CTAG	7 cut(s)	86, 645, 860, 1301, 1332, 1367, 1500
SstI	GAGCTC	1 cut(s)	303
StyD4I	CCNGG	7 cut(s)	751, 752, 1058, 1156, 1416, 1618, 1648
StyI	CCWWGG	2 cut(s)	579, 1786
TaaI	ACNGT	6 cut(s)	350, 490, 1116, 1219, 1519, 1864
TaiI	ACGT	1 cut(s)	485
TaqI	TCGA	8 cut(s)	256, 309, 432, 437, 735, 765, 820, 1242
TatI	WGTACW	1 cut(s)	567
TauI	GCSGC	4 cut(s)	8, 398, 703, 1266
TfiI	GAWTC	2 cut(s)	434, 1145
TscAI	CASTG	2 cut(s)	1147, 1806
TseFI	GTSAC	4 cut(s)	1136, 1468, 1480, 1729
TseI	GCWGC	1 cut(s)	1313
Tsp45I	GTSAC	4 cut(s)	1136, 1468, 1480, 1729
TspDTI	ATGAA	5 cut(s)	32, 216, 627, 632, 1862
TspGWI	ACGGA	4 cut(s)	399, 689, 762, 1187
TspMI	CCCGGG	1 cut(s)	752
TspRI	CASTG	2 cut(s)	1147, 1806
VneI	GTGCAC	1 cut(s)	402
VpaK11BI	GGWCC	5 cut(s)	118, 710, 749, 827, 928
XapI	RAATTY	6 cut(s)	107, 531, 974, 1250, 1557, 1945
XbaI	TCTAGA	1 cut(s)	859
XceI	RCATGY	1 cut(s)	2000
XcmI	CCANNNNNNNNNTGG	1 cut(s)	939
XmaI	CCCGGG	1 cut(s)	752
XmnI	GAANNNNTTC	1 cut(s)	1896
XspI	CTAG	7 cut(s)	86, 645, 860, 1301, 1332, 1367, 1500
Zsp2I	ATGCAT	2 cut(s)	1051, 1103

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.