Rroxscaffold_2G00090210

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001139
Physical Location & Seq
Reverse (-)
11989243 .. 11993017
3775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00090210.1

Sequence Viewer

Length: 216 bp
ATGCGGGATGTTCGGGTTCCCAATACCGCCCTAAGGGGTATCTCAACCCCCCGGCGACTACAACCTCTCGAGATCGGGATCGATGCCTTGATCCGATATCGAGTCCGCCGCAACGGCGAGCATCTTGGCACCTATGTCGGCTATAACGGCGCCGTTTGGTGCTGCGACATTTGGCTTGGTTCACCAAAGAAGCCCACCGAGTCAAATTTCCGGTGA

Protein Analysis

71

Amino Acids

8.14

Weight (kDa)

10.17

Isoelectric Point (pI)

34.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 128, 149
AciI CCGC 4 cut(s) 4, 27, 106, 109
AclWI GGATC 2 cut(s) 85, 86
AcsI RAATTY 1 cut(s) 205
AcyI GRCGYC 1 cut(s) 150
AfiI CCNNNNNNNGG 1 cut(s) 33
AlwI GGATC 2 cut(s) 85, 86
Ama87I CYCGRG 1 cut(s) 68
ApeKI GCWGC 1 cut(s) 162
ApoI RAATTY 1 cut(s) 205
AspLEI GCGC 1 cut(s) 152
AsuC2I CCSGG 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 174
AvaI CYCGRG 1 cut(s) 68
AxyI CCTNAGG 1 cut(s) 32
BanI GGYRCC 2 cut(s) 128, 149
BbvI GCAGC 1 cut(s) 149
BceAI ACGGC 3 cut(s) 130, 137, 163
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BcnI CCSGG 1 cut(s) 52
BfoI RGCGCY 1 cut(s) 153
BglI GCCNNNNNGGC 1 cut(s) 114
BisI GCNGC 2 cut(s) 109, 163
BlsI GCNGC 2 cut(s) 110, 164
Bme1390I CCNGG 1 cut(s) 52
BmeT110I CYCGRG 1 cut(s) 68
BmiI GGNNCC 3 cut(s) 18, 130, 151
BmrFI CCNGG 1 cut(s) 52
BmsI GCATC 2 cut(s) 73, 130
BpuMI CCSGG 1 cut(s) 52
Bsa29I ATCGAT 1 cut(s) 81
BsaBI GATNNNNATC 1 cut(s) 77
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 50
BsaWI WCCGGW 1 cut(s) 210
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse21I CCTNAGG 1 cut(s) 32
Bse8I GATNNNNATC 1 cut(s) 77
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 50
BseGI GGATG 1 cut(s) 13
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 1 cut(s) 33
BseXI GCAGC 1 cut(s) 149
BshNI GGYRCC 2 cut(s) 128, 149
BshVI ATCGAT 1 cut(s) 81
BsiHKCI CYCGRG 1 cut(s) 68
BsiSI CCGG 2 cut(s) 52, 211
BslI CCNNNNNNNGG 1 cut(s) 33
BsoBI CYCGRG 1 cut(s) 68
Bsp143I GATC 3 cut(s) 72, 78, 90
BspACI CCGC 4 cut(s) 4, 27, 106, 109
BspDI ATCGAT 1 cut(s) 81
BspLI GGNNCC 3 cut(s) 18, 130, 151
BspPI GGATC 2 cut(s) 85, 86
BspT107I GGYRCC 2 cut(s) 128, 149
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 3 cut(s) 72, 78, 90
BssNI GRCGYC 1 cut(s) 150
BstACI GRCGYC 1 cut(s) 150
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 1 cut(s) 32
BstF5I GGATG 1 cut(s) 13
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 3 cut(s) 75, 81, 93
BstMBI GATC 3 cut(s) 72, 78, 90
BstMWI GCNNNNNNNGC 2 cut(s) 114, 147
BstSCI CCNGG 1 cut(s) 50
BstV1I GCAGC 1 cut(s) 149
Bsu15I ATCGAT 1 cut(s) 81
Bsu36I CCTNAGG 1 cut(s) 32
BsuTUI ATCGAT 1 cut(s) 81
BtsCI GGATG 1 cut(s) 13
Cac8I GCNNGC 1 cut(s) 119
CfoI GCGC 1 cut(s) 152
ClaI ATCGAT 1 cut(s) 81
CviJI RGCY 3 cut(s) 141, 175, 193
CviKI_1 RGCY 3 cut(s) 141, 175, 193
DdeI CTNAG 1 cut(s) 32
DinI GGCGCC 1 cut(s) 151
DpnI GATC 3 cut(s) 74, 80, 92
DpnII GATC 3 cut(s) 72, 78, 90
EciI GGCGGA 1 cut(s) 95
Eco32I GATATC 1 cut(s) 98
Eco81I CCTNAGG 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 68
EcoRV GATATC 1 cut(s) 98
EgeI GGCGCC 1 cut(s) 151
EheI GGCGCC 1 cut(s) 151
FaiI YATR 2 cut(s) 135, 144
Fnu4HI GCNGC 2 cut(s) 109, 163
FokI GGATG 1 cut(s) 20
Fsp4HI GCNGC 2 cut(s) 109, 163
GlaI GCGC 1 cut(s) 151
GluI GCNGC 2 cut(s) 109, 163
HaeII RGCGCY 1 cut(s) 153
HapII CCGG 2 cut(s) 52, 211
HhaI GCGC 1 cut(s) 152
Hin1I GRCGYC 1 cut(s) 150
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HinfI GANTC 2 cut(s) 102, 200
HpaII CCGG 2 cut(s) 52, 211
HphI GGTGA 1 cut(s) 174
Hpy166II GTNNAC 1 cut(s) 182
Hpy188I TCNGA 1 cut(s) 95
Hpy188III TCNNGA 3 cut(s) 68, 70, 76
Hpy8I GTNNAC 1 cut(s) 182
HpyF10VI GCNNNNNNNGC 2 cut(s) 114, 147
HpyF3I CTNAG 1 cut(s) 32
Hsp92I GRCGYC 1 cut(s) 150
HspAI GCGC 1 cut(s) 150
KasI GGCGCC 1 cut(s) 149
Kzo9I GATC 3 cut(s) 72, 78, 90
LpnPI CCDG 1 cut(s) 65
Lsp1109I GCAGC 1 cut(s) 149
LweI GCATC 2 cut(s) 73, 130
MalI GATC 3 cut(s) 74, 80, 92
MboI GATC 3 cut(s) 72, 78, 90
MluCI AATT 1 cut(s) 205
Mly113I GGCGCC 1 cut(s) 150
MlyI GAGTC 2 cut(s) 111, 209
MnlI CCTC 1 cut(s) 75
MspI CCGG 2 cut(s) 52, 211
MspR9I CCNGG 1 cut(s) 52
MwoI GCNNNNNNNGC 2 cut(s) 114, 147
NarI GGCGCC 1 cut(s) 150
NciI CCSGG 1 cut(s) 52
NdeII GATC 3 cut(s) 72, 78, 90
NlaIV GGNNCC 3 cut(s) 18, 130, 151
PaeR7I CTCGAG 1 cut(s) 68
PkrI GCNGC 2 cut(s) 110, 164
PleI GAGTC 2 cut(s) 110, 208
PluTI GGCGCC 1 cut(s) 153
PpsI GAGTC 2 cut(s) 110, 208
PspN4I GGNNCC 3 cut(s) 18, 130, 151
SatI GCNGC 2 cut(s) 109, 163
Sau3AI GATC 3 cut(s) 72, 78, 90
SchI GAGTC 2 cut(s) 111, 209
ScrFI CCNGG 1 cut(s) 52
SetI ASST 2 cut(s) 67, 134
SfaNI GCATC 2 cut(s) 73, 130
SfoI GGCGCC 1 cut(s) 151
Sfr274I CTCGAG 1 cut(s) 68
SlaI CTCGAG 1 cut(s) 68
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 1 cut(s) 205
SsiI CCGC 4 cut(s) 4, 27, 106, 109
SspDI GGCGCC 1 cut(s) 149
StyD4I CCNGG 1 cut(s) 50
TaqI TCGA 3 cut(s) 69, 81, 100
TasI AATT 1 cut(s) 205
TauI GCSGC 1 cut(s) 111
TseI GCWGC 1 cut(s) 162
XapI RAATTY 1 cut(s) 205
XhoI CTCGAG 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.