MD17G1186800.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000496
Physical Location & Seq
Reverse (-)
22326193 .. 22328268
2076 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1186800.v1.1.491

Sequence Viewer

Length: 1890 bp
ATGGATCCAAGGCTTTTAGAGTCAATTACCAGTAACAACGTACCTGCATTTATCGCATTAGTTCGCGAAAATGAAGGACTTGTTGAGCAAAGAACAGCTAATGATTCCTTGAACACGGTTTTACACATTGCTACTAAGTTCGGATGCATCGACATTGTGTCCGAAATTGTTAGGTTGTGTCCTGAAATGGTTGCTGCTGAAAACAAACACCTAGAGACTTCGGTACATGAAGCTTGTCGCCTGGGAAATGCTAGCGTCTTAAAGCTACTCTTAGAAGCCAATCCCCGGGCAGCCTGTAAGCTTAACTCCGACAAGAGGAGTGCTGCCTTTGTAGCTTGCAGGCTTGGCCATTTTGATGTGGTAAATCTCTTATTGAATCAGTCCGGGCTTGAAGGCTTAGAGGACATTGGAATTGACGAAACATGTATTCATATCGCTGCATCCAGCGGACACACAGATATTGTTCGAGGGATCCTAGACATGTGCTCCGACTACGCTCAAAAGACCGATGCGAAAGGAAATTCACCCTTGCACTACGCTTGCAGCAAAGGGCACAGGGAGATAACTTGGATGCTCCTGCAGCGAGATGTGAATCTGGCATTGCAGTATAACAACAACGGTTACACGCCTTTGCATTTGGCAGCAATACGTGGAAATATTTCAGTCCTTGAAGACTTTGCATTGAAGACTCCTGCAGCTTTCCACTATCTGACTAAAGAAGAAGAGACGTTATTTCATCTGACCGTGCGATATGGAAAATTTGAAGCTTTGTTATTCTTGATGCATGTCGCTAACAGCTTCAATCTATTTCAGTGTCAAGATCGGTATGGAAACAGCATTTTACATCTTGCAGTTTCTGGCGGGCATTATGAGTTCTTCCAGATTGCTGAGTACTTGATCAACAAAGCAAAAGTGGAGATCAATTCACGAAACTGCAAAGGACTTACGGCGTTTGACATCCTCAACCAGGCCAAGGACAGTGCAGACACTCGAAATCTTCAAACCATATTCCATAAAGCTGGTGGTGAAAAAAGTACTAACATGACATCTTGTTCACCAGAAACATTGTCTTCAAGGCAGAGTTACATATTCGAATCAGATATGTCTAATGCAAACGAAAATGGTATTCTCCCGTCTTTGCCAGAAATCACCTACACTCCGCAAGAGGATCAGTACAGGTCTAAAATGTCATCGCTCCATAATCATGTTCATGAGAGATTTGAGAAGGAAGCATACAAACTGGAGTCTTCATCCCCAACTAATTCACGTCAACGCAAGCCTTCCAGCAAAAGGCACTGTGAATACCGTAAGGAGCATAAGATATACAGAGAGGCGATACAAAATGCTAGGAACACTATAACGCTAGTTGCAATTCTGATTGCAACAGTTGCTTTTACTGCTGGAATTAGCCCTCCTGGCGGTGTACATCAGGACGGAACAATGAAAGGGAAAGCGGTGGCAGGCAGAACAACAGCTTTCAAGGTCTTTGCAATAAGCAACGACGTTGCGCTCTTCACGTCACTCTCTATAGTTGTTTTTCTTGTCAGTATCATCCCCTATCGAAGAAAACCGCAGATGATGTTGCTGGTGGTTGCTCACAAGGTCATGTGGGTATCTGTGGCATTCATGGCGACTGCGTATGTTGCAGCAACGTGGGTGATTATGCCACGCAACCACGGTACAGCATTTGTCCTGGTGACGCTACTAGCAGTTGGTGGCGGCACTCTTGGGACAATCTTCGTCGGTCTTGGAGTGATGCTGGTTGATCATTGGCTTAGAAAGTTGAAATGGAGGAAACTGAAAATGGGAATATGGAGATTTGCAGATGGAGTGGAAAGCCAAAATTCTGATGTTGAAAGCTCCTTCCATCAAGGATATCACTCCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

630

Amino Acids

70.06

Weight (kDa)

8.32

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 41 - 127 1e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 76 - 160 1.1e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 139 - 198 1.2e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 143 - 223 8.6e-15 Ankyrin repeats (3 copies)
Ank_4 PF13637 143 - 192 6.6e-06 Ankyrin repeats (many copies)
PGG PF13962 444 - 553 1.4e-30 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10340 AT1G10340 AT1G34046 AT1G34050 AT2G24600 AT2G24600 AT2G24600 AT2G24600 AT3G52830 AT5G54700 AT5G54700
fragaria_vesca FvH4_6g32091 FvH4_6g32100 FvH4_6g32100 FvH4_6g32121
malus_domestica MD17G1186800.v1.1 MD17G1187000.v1.1
prunus_persica Prupe.3G061900_v2.0.a1 Prupe.3G062300_v2.0.a1 Prupe.3G062300_v2.0.a1 Prupe.3G062300_v2.0.a1 Prupe.3G062400_v2.0.a1 Prupe.3G065300_v2.0.a1
pyrus_communis pycom17g19590
rosa_chinensis RchiOBHm_Chr1g0353591 RchiOBHm_Chr2g0139761 RchiOBHm_Chr2g0139771 RchiOBHm_Chr2g0139841 RchiOBHm_Chr2g0139851 RchiOBHm_Chr2g0139911
rosa_laevigata RLG00000019831 RLG00000019832 RLG00000019834 RLG00000019835 RLG00000019838 RLG00000028268 RLG00000029493 RLG00000029500 RLG00000029519 RLG00000029774
rosa_multiflora Rmu_co8197386.1_g000001 Rmu_co8359109.1_g000001 Rmu_sc0000545.1_g000005 Rmu_sc0005914.1_g000001 Rmu_sc0030896.1_g000002 Rmu_sc0030896.1_g000003 Rmu_sc0030896.1_g000004 Rmu_sc0032265.1_g000001
rosa_roxburghii Rroxscaffold_2G00105170 Rroxscaffold_2G00105210 Rroxscaffold_2G00105220 Rroxscaffold_4G00301800
rosa_rugosa Rorug01G0236400 Rorug02G0359900 Rorug02G0360000 Rorug02G0360200
rosa_samantha Rh1AG248700 Rh1BG105500 Rh1BG219100 Rh1CG231800 Rh1DG245500 Rh2AG409600 Rh2AG409700 Rh2AG410000 Rh2AG410100 Rh2AG410400 Rh2AG410500 Rh2BG420100 Rh2BG420200 Rh2BG420600 Rh2BG420700 Rh2CG395900 Rh2CG396000 Rh2CG396200 Rh2CG396500 Rh2CG396600 Rh2DG429400 Rh2DG429500 Rh2DG429800 Rh2DG430100 Rh2DG430200
rosa_wichuraiana Rw1G021690 Rw2G033350 Rw2G033400 Rw2G033410 Rw2G033570 Rw2G033620 Rw2G033630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 52
AccII CGCG 1 cut(s) 66
AciI CCGC 7 cut(s) 447, 861, 1160, 1419, 1454, 1571, 1719
AclWI GGATC 4 cut(s) 12, 466, 479, 1176
AcoI YGGCCR 1 cut(s) 346
AcsI RAATTY 3 cut(s) 520, 758, 1843
AfaI GTAC 7 cut(s) 42, 225, 893, 1036, 1175, 1425, 1681
AfiI CCNNNNNNNGG 6 cut(s) 285, 315, 967, 973, 1290, 1418
AflIII ACRYGT 2 cut(s) 422, 480
AhdI GACNNNNNGTC 1 cut(s) 157
AjiI CACGTC 2 cut(s) 1268, 1518
AjnI CCWGG 4 cut(s) 240, 966, 1414, 1692
Alw21I GWGCWC 1 cut(s) 488
Alw26I GTCTC 2 cut(s) 209, 719
AlwI GGATC 4 cut(s) 12, 466, 479, 1176
AlwNI CAGNNNCTG 1 cut(s) 857
Ama87I CYCGRG 1 cut(s) 285
AoxI GGCC 2 cut(s) 346, 969
ApeKI GCWGC 9 cut(s) 194, 290, 323, 437, 543, 580, 641, 695, 1646
ApoI RAATTY 3 cut(s) 520, 758, 1843
Asp700I GAANNNNTTC 1 cut(s) 658
AspLEI GCGC 1 cut(s) 1510
AsuC2I CCSGG 3 cut(s) 286, 287, 385
AsuHPI GGTGA 6 cut(s) 516, 1037, 1047, 1141, 1669, 1708
AsuII TTCGAA 1 cut(s) 1092
AsuNHI GCTAGC 1 cut(s) 251
AvaI CYCGRG 1 cut(s) 285
BaeGI GKGCMC 1 cut(s) 555
BalI TGGCCA 1 cut(s) 348
BamHI GGATCC 2 cut(s) 4, 471
BarI GAAGNNNNNNTAC 2 cut(s) 1217, 1249
BbsI GAAGAC 4 cut(s) 678, 692, 1062, 1239
Bbv12I GWGCWC 1 cut(s) 488
BbvI GCAGC 9 cut(s) 181, 302, 310, 424, 555, 592, 653, 707, 1658
BccI CCATC 2 cut(s) 1820, 1875
BceAI ACGGC 1 cut(s) 963
BciT130I CCWGG 4 cut(s) 242, 968, 1416, 1694
BclI TGATCA 2 cut(s) 897, 1765
BcnI CCSGG 3 cut(s) 286, 287, 385
BcoDI GTCTC 2 cut(s) 209, 719
BfaI CTAG 6 cut(s) 212, 252, 476, 1347, 1364, 1706
BfmI CTRYAG 3 cut(s) 578, 693, 1527
BfuAI ACCTGC 1 cut(s) 52
BglI GCCNNNNNGGC 1 cut(s) 1416
BmcAI AGTACT 2 cut(s) 893, 1036
Bme1390I CCNGG 7 cut(s) 242, 286, 287, 385, 968, 1416, 1694
BmeRI GACNNNNNGTC 1 cut(s) 157
BmeT110I CYCGRG 1 cut(s) 285
BmgBI CACGTC 2 cut(s) 1268, 1518
BmiI GGNNCC 2 cut(s) 6, 473
BmrFI CCNGG 7 cut(s) 242, 286, 287, 385, 968, 1416, 1694
BmsI GCATC 7 cut(s) 134, 156, 449, 499, 561, 771, 1746
BmtI GCTAGC 1 cut(s) 255
BpiI GAAGAC 4 cut(s) 678, 692, 1062, 1239
BpmI CTGGAG 1 cut(s) 1262
Bpu14I TTCGAA 1 cut(s) 1092
BpuMI CCSGG 3 cut(s) 286, 287, 385
BsaAI YACGTR 1 cut(s) 650
BsaBI GATNNNNATC 1 cut(s) 591
BsaJI CCNNGG 6 cut(s) 8, 241, 284, 285, 972, 1675
BsaXI ACNNNNNCTCC 4 cut(s) 470, 500, 1141, 1171
Bsc4I CCNNNNNNNGG 6 cut(s) 285, 315, 967, 973, 1290, 1418
Bse1I ACTGG 2 cut(s) 30, 1245
Bse3DI GCAATG 2 cut(s) 126, 599
Bse8I GATNNNNATC 1 cut(s) 591
BseBI CCWGG 4 cut(s) 242, 968, 1416, 1694
BseDI CCNNGG 6 cut(s) 8, 241, 284, 285, 972, 1675
BseGI GGATG 6 cut(s) 149, 440, 576, 957, 1250, 1551
BseJI GATNNNNATC 1 cut(s) 591
BseLI CCNNNNNNNGG 6 cut(s) 285, 315, 967, 973, 1290, 1418
BseMI GCAATG 2 cut(s) 126, 599
BseMII CTCAG 1 cut(s) 879
BseNI ACTGG 2 cut(s) 30, 1245
BseRI GAGGAG 1 cut(s) 331
BseSI GKGCMC 1 cut(s) 555
BseXI GCAGC 9 cut(s) 181, 302, 310, 424, 555, 592, 653, 707, 1658
BsgI GTGCAG 1 cut(s) 1002
Bsh1236I CGCG 1 cut(s) 66
BshFI GGCC 2 cut(s) 348, 971
BsiHKAI GWGCWC 1 cut(s) 488
BsiHKCI CYCGRG 1 cut(s) 285
BsiSI CCGG 2 cut(s) 286, 384
BslFI GGGAC 1 cut(s) 1744
BslI CCNNNNNNNGG 6 cut(s) 285, 315, 967, 973, 1290, 1418
BsmAI GTCTC 2 cut(s) 209, 719
BsmBI CGTCTC 1 cut(s) 719
BsmFI GGGAC 1 cut(s) 1744
BsmI GAATGC 1 cut(s) 1622
BsnI GGCC 2 cut(s) 348, 971
BsoBI CYCGRG 1 cut(s) 285
Bsp119I TTCGAA 1 cut(s) 1092
Bsp1286I GDGCHC 2 cut(s) 488, 555
Bsp1407I TGTACA 1 cut(s) 1423
Bsp143I GATC 7 cut(s) 4, 471, 820, 897, 918, 1168, 1765
Bsp68I TCGCGA 1 cut(s) 66
BspACI CCGC 7 cut(s) 447, 861, 1160, 1419, 1454, 1571, 1719
BspANI GGCC 2 cut(s) 348, 971
BspCNI CTCAG 1 cut(s) 880
BspFNI CGCG 1 cut(s) 66
BspHI TCATGA 1 cut(s) 1210
BspLI GGNNCC 2 cut(s) 6, 473
BspMAI CTGCAG 2 cut(s) 582, 697
BspMI ACCTGC 1 cut(s) 52
BspOI GCTAGC 1 cut(s) 255
BspPI GGATC 4 cut(s) 12, 466, 479, 1176
BspQI GCTCTTC 1 cut(s) 1517
BspT104I TTCGAA 1 cut(s) 1092
BsrDI GCAATG 2 cut(s) 126, 599
BsrGI TGTACA 1 cut(s) 1423
BsrI ACTGG 2 cut(s) 30, 1245
BssECI CCNNGG 6 cut(s) 8, 241, 284, 285, 972, 1675
BssMI GATC 7 cut(s) 4, 471, 820, 897, 918, 1168, 1765
BssT1I CCWWGG 2 cut(s) 8, 972
Bst2UI CCWGG 4 cut(s) 242, 968, 1416, 1694
Bst4CI ACNGT 8 cut(s) 118, 620, 745, 980, 1298, 1307, 1387, 1679
Bst6I CTCTTC 2 cut(s) 717, 1517
BstAPI GCANNNNNTGC 1 cut(s) 1388
BstAUI TGTACA 1 cut(s) 1423
BstBAI YACGTR 1 cut(s) 650
BstBI TTCGAA 1 cut(s) 1092
BstC8I GCNNGC 7 cut(s) 253, 337, 341, 541, 863, 1277, 1462
BstDEI CTNAG 5 cut(s) 135, 271, 397, 888, 1775
BstDSI CCRYGG 1 cut(s) 1675
BstENI CCTNNNNNAGG 1 cut(s) 965
BstF5I GGATG 6 cut(s) 149, 440, 576, 957, 1250, 1551
BstFNI CGCG 1 cut(s) 66
BstHHI GCGC 1 cut(s) 1510
BstKTI GATC 7 cut(s) 7, 474, 823, 900, 921, 1171, 1768
BstMAI GTCTC 2 cut(s) 209, 719
BstMBI GATC 7 cut(s) 4, 471, 820, 897, 918, 1168, 1765
BstMWI GCNNNNNNNGC 9 cut(s) 53, 332, 345, 580, 1388, 1397, 1416, 1628, 1643
BstNI CCWGG 4 cut(s) 242, 968, 1416, 1694
BstNSI RCATGY 3 cut(s) 426, 484, 788
BstSCI CCNGG 7 cut(s) 240, 284, 285, 383, 966, 1414, 1692
BstSFI CTRYAG 3 cut(s) 578, 693, 1527
BstSLI GKGCMC 1 cut(s) 555
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 9 cut(s) 181, 302, 310, 424, 555, 592, 653, 707, 1658
BstV2I GAAGAC 4 cut(s) 678, 692, 1062, 1239
BstX2I RGATCY 2 cut(s) 4, 471
BstXI CCANNNNNNTGG 1 cut(s) 1019
BstYI RGATCY 2 cut(s) 4, 471
BsuRI GGCC 2 cut(s) 348, 971
BtgI CCRYGG 1 cut(s) 1675
BtgZI GCGATG 1 cut(s) 1176
BtrI CACGTC 2 cut(s) 1268, 1518
BtsCI GGATG 6 cut(s) 149, 440, 576, 957, 1250, 1551
BtsIMutI CAGTG 3 cut(s) 818, 985, 1294
BtuMI TCGCGA 1 cut(s) 66
BveI ACCTGC 1 cut(s) 52
Cac8I GCNNGC 7 cut(s) 253, 337, 341, 541, 863, 1277, 1462
CaiI CAGNNNCTG 1 cut(s) 857
CciI TCATGA 1 cut(s) 1210
CfoI GCGC 1 cut(s) 1510
Cfr9I CCCGGG 1 cut(s) 285
CseI GACGC 2 cut(s) 244, 1708
Csp6I GTAC 7 cut(s) 41, 224, 892, 1035, 1174, 1424, 1680
CviAII CATG 9 cut(s) 227, 423, 481, 785, 1042, 1205, 1211, 1606, 1627
CviQI GTAC 7 cut(s) 41, 224, 892, 1035, 1174, 1424, 1680
DdeI CTNAG 5 cut(s) 135, 271, 397, 888, 1775
DpnI GATC 7 cut(s) 6, 473, 822, 899, 920, 1170, 1767
DpnII GATC 7 cut(s) 4, 471, 820, 897, 918, 1168, 1765
DriI GACNNNNNGTC 1 cut(s) 157
EaeI YGGCCR 1 cut(s) 346
Eam1104I CTCTTC 2 cut(s) 717, 1517
Eam1105I GACNNNNNGTC 1 cut(s) 157
EarI CTCTTC 2 cut(s) 717, 1517
Eco130I CCWWGG 2 cut(s) 8, 972
Eco32I GATATC 1 cut(s) 1877
Eco88I CYCGRG 1 cut(s) 285
EcoNI CCTNNNNNAGG 1 cut(s) 965
EcoRII CCWGG 4 cut(s) 240, 966, 1414, 1692
EcoRV GATATC 1 cut(s) 1877
EcoT14I CCWWGG 2 cut(s) 8, 972
EcoT22I ATGCAT 2 cut(s) 149, 786
ErhI CCWWGG 2 cut(s) 8, 972
Esp3I CGTCTC 1 cut(s) 719
FaeI CATG 9 cut(s) 230, 426, 484, 788, 1045, 1208, 1214, 1609, 1630
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FaqI GGGAC 1 cut(s) 1744
FatI CATG 9 cut(s) 226, 422, 480, 784, 1041, 1204, 1210, 1605, 1626
FauI CCCGC 1 cut(s) 854
FbaI TGATCA 2 cut(s) 897, 1765
FokI GGATG 6 cut(s) 156, 427, 583, 944, 1237, 1538
FspBI CTAG 6 cut(s) 212, 252, 476, 1347, 1364, 1706
GlaI GCGC 1 cut(s) 1509
GsuI CTGGAG 1 cut(s) 1262
HaeIII GGCC 2 cut(s) 348, 971
HapII CCGG 2 cut(s) 286, 384
HgaI GACGC 2 cut(s) 244, 1708
HhaI GCGC 1 cut(s) 1510
Hin1II CATG 9 cut(s) 230, 426, 484, 788, 1045, 1208, 1214, 1609, 1630
Hin6I GCGC 1 cut(s) 1508
HinP1I GCGC 1 cut(s) 1508
HincII GTYRAC 1 cut(s) 1271
HindII GTYRAC 1 cut(s) 1271
HindIII AAGCTT 3 cut(s) 231, 299, 765
HinfI GANTC 7 cut(s) 20, 104, 376, 592, 688, 1094, 1244
HpaII CCGG 2 cut(s) 286, 384
HphI GGTGA 6 cut(s) 516, 1037, 1047, 1141, 1669, 1708
Hpy166II GTNNAC 3 cut(s) 1055, 1271, 1424
Hpy188I TCNGA 9 cut(s) 143, 163, 310, 490, 711, 741, 1099, 1377, 1849
Hpy188III TCNNGA 8 cut(s) 65, 182, 778, 818, 880, 927, 1211, 1430
Hpy8I GTNNAC 3 cut(s) 1055, 1271, 1424
Hpy99I CGWCG 2 cut(s) 1505, 1745
HpyAV CCTTC 5 cut(s) 68, 386, 1219, 1290, 1873
HpyCH4III ACNGT 8 cut(s) 118, 620, 745, 980, 1298, 1307, 1387, 1679
HpyCH4IV ACGT 7 cut(s) 39, 649, 728, 1267, 1503, 1517, 1652
HpyF10VI GCNNNNNNNGC 9 cut(s) 53, 332, 345, 580, 1388, 1397, 1416, 1628, 1643
HpyF3I CTNAG 5 cut(s) 135, 271, 397, 888, 1775
HpySE526I ACGT 7 cut(s) 39, 649, 728, 1267, 1503, 1517, 1652
Hsp92II CATG 9 cut(s) 230, 426, 484, 788, 1045, 1208, 1214, 1609, 1630
HspAI GCGC 1 cut(s) 1508
Ksp22I TGATCA 2 cut(s) 897, 1765
Kzo9I GATC 7 cut(s) 4, 471, 820, 897, 918, 1168, 1765
LguI GCTCTTC 1 cut(s) 1517
LmnI GCTCC 5 cut(s) 491, 579, 1200, 1312, 1865
Lsp1109I GCAGC 9 cut(s) 181, 302, 310, 424, 555, 592, 653, 707, 1658
LweI GCATC 7 cut(s) 134, 156, 449, 499, 561, 771, 1746
MaeI CTAG 6 cut(s) 212, 252, 476, 1347, 1364, 1706
MaeII ACGT 7 cut(s) 39, 649, 728, 1267, 1503, 1517, 1652
MaeIII GTNAC 5 cut(s) 32, 620, 1082, 1518, 1696
MalI GATC 7 cut(s) 6, 473, 822, 899, 920, 1170, 1767
MboI GATC 7 cut(s) 4, 471, 820, 897, 918, 1168, 1765
MflI RGATCY 2 cut(s) 4, 471
MhlI GDGCHC 2 cut(s) 488, 555
MlsI TGGCCA 1 cut(s) 348
MluNI TGGCCA 1 cut(s) 348
MlyI GAGTC 3 cut(s) 29, 682, 1253
MmeI TCCRAC 2 cut(s) 333, 513
MnlI CCTC 8 cut(s) 309, 394, 461, 971, 1159, 1324, 1422, 1785
Mox20I TGGCCA 1 cut(s) 348
Mph1103I ATGCAT 2 cut(s) 149, 786
MroXI GAANNNNTTC 1 cut(s) 658
MscI TGGCCA 1 cut(s) 348
MseI TTAA 2 cut(s) 260, 303
MslI CAYNNNNRTG 3 cut(s) 354, 1203, 1209
Msp20I TGGCCA 1 cut(s) 348
MspA1I CMGCKG 1 cut(s) 447
MspI CCGG 2 cut(s) 286, 384
MspR9I CCNGG 7 cut(s) 242, 286, 287, 385, 968, 1416, 1694
Mva1269I GAATGC 1 cut(s) 1622
MvaI CCWGG 4 cut(s) 242, 968, 1416, 1694
MvnI CGCG 1 cut(s) 66
MwoI GCNNNNNNNGC 9 cut(s) 53, 332, 345, 580, 1388, 1397, 1416, 1628, 1643
NciI CCSGG 3 cut(s) 286, 287, 385
NdeII GATC 7 cut(s) 4, 471, 820, 897, 918, 1168, 1765
NheI GCTAGC 1 cut(s) 251
NlaIII CATG 9 cut(s) 230, 426, 484, 788, 1045, 1208, 1214, 1609, 1630
NlaIV GGNNCC 2 cut(s) 6, 473
NmuCI GTSAC 2 cut(s) 1518, 1696
NruI TCGCGA 1 cut(s) 66
NsiI ATGCAT 2 cut(s) 149, 786
NspI RCATGY 3 cut(s) 426, 484, 788
NspV TTCGAA 1 cut(s) 1092
PagI TCATGA 1 cut(s) 1210
PciI ACATGT 2 cut(s) 422, 480
PciSI GCTCTTC 1 cut(s) 1517
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PctI GAATGC 1 cut(s) 1622
PdmI GAANNNNTTC 1 cut(s) 658
PfeI GAWTC 4 cut(s) 104, 376, 592, 1094
PleI GAGTC 3 cut(s) 28, 682, 1252
PpsI GAGTC 3 cut(s) 28, 682, 1252
Ppu21I YACGTR 1 cut(s) 650
PscI ACATGT 2 cut(s) 422, 480
Psp6I CCWGG 4 cut(s) 240, 966, 1414, 1692
PspGI CCWGG 4 cut(s) 240, 966, 1414, 1692
PspN4I GGNNCC 2 cut(s) 6, 473
PstI CTGCAG 2 cut(s) 582, 697
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 2 cut(s) 4, 471
RruI TCGCGA 1 cut(s) 66
RsaI GTAC 7 cut(s) 42, 225, 893, 1036, 1175, 1425, 1681
RsaNI GTAC 7 cut(s) 41, 224, 892, 1035, 1174, 1424, 1680
RseI CAYNNNNRTG 3 cut(s) 354, 1203, 1209
SapI GCTCTTC 1 cut(s) 1517
SaqAI TTAA 2 cut(s) 260, 303
Sau3AI GATC 7 cut(s) 4, 471, 820, 897, 918, 1168, 1765
ScaI AGTACT 2 cut(s) 893, 1036
SchI GAGTC 3 cut(s) 29, 682, 1253
ScrFI CCNGG 7 cut(s) 242, 286, 287, 385, 968, 1416, 1694
SduI GDGCHC 2 cut(s) 488, 555
SfaNI GCATC 7 cut(s) 134, 156, 449, 499, 561, 771, 1746
SfcI CTRYAG 3 cut(s) 578, 693, 1527
SfuI TTCGAA 1 cut(s) 1092
SmaI CCCGGG 1 cut(s) 287
SmiMI CAYNNNNRTG 3 cut(s) 354, 1203, 1209
SsiI CCGC 7 cut(s) 447, 861, 1160, 1419, 1454, 1571, 1719
SspI AATATT 1 cut(s) 658
SspMI CTAG 6 cut(s) 212, 252, 476, 1347, 1364, 1706
StyD4I CCNGG 7 cut(s) 240, 284, 285, 383, 966, 1414, 1692
StyI CCWWGG 2 cut(s) 8, 972
TaaI ACNGT 8 cut(s) 118, 620, 745, 980, 1298, 1307, 1387, 1679
TaiI ACGT 7 cut(s) 42, 652, 731, 1270, 1506, 1520, 1655
TaqI TCGA 5 cut(s) 150, 466, 991, 1092, 1561
TaqII GACCGA 2 cut(s) 521, 1733
TatI WGTACW 4 cut(s) 891, 1034, 1173, 1423
TauI GCSGC 1 cut(s) 1722
TfiI GAWTC 4 cut(s) 104, 376, 592, 1094
Tru1I TTAA 2 cut(s) 260, 303
Tru9I TTAA 2 cut(s) 260, 303
TscAI CASTG 3 cut(s) 818, 985, 1301
TseFI GTSAC 2 cut(s) 1518, 1696
TseI GCWGC 9 cut(s) 194, 290, 323, 437, 543, 580, 641, 695, 1646
Tsp45I GTSAC 2 cut(s) 1518, 1696
TspDTI ATGAA 8 cut(s) 87, 243, 419, 725, 1199, 1239, 1457, 1615
TspGWI ACGGA 1 cut(s) 1449
TspMI CCCGGG 1 cut(s) 285
TspRI CASTG 3 cut(s) 818, 985, 1301
XagI CCTNNNNNAGG 1 cut(s) 965
XapI RAATTY 3 cut(s) 520, 758, 1843
XceI RCATGY 3 cut(s) 426, 484, 788
XcmI CCANNNNNNNNNTGG 1 cut(s) 1019
XmaI CCCGGG 1 cut(s) 285
XmnI GAANNNNTTC 1 cut(s) 658
XspI CTAG 6 cut(s) 212, 252, 476, 1347, 1364, 1706
ZrmI AGTACT 2 cut(s) 893, 1036
Zsp2I ATGCAT 2 cut(s) 149, 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.