RLG00000028268

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000496
Physical Location & Seq
Reverse (-)
20701340 .. 20704083
2744 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028268

Sequence Viewer

Length: 2103 bp
ATGGATTTCAGGATTTTAGAGGCAATTGAGAGAAAGGACATAGTCACATTTGCTAGCTTAGTCCGAGAAAATGAAGGAATTCTCGAGCAAAGAGAAGATGATACTCTGAACACGCCATTACACCTTGTGTCAAAGTTTGGATGCCTTGAAATGGCATCTGAAATTGTTTCTTTGTGCCCGGACATGGTTGCTGCTGAGAATAAGAACCAGGAGACCCCAGTCCATGAAGCTTGTCGAACAGGGAATCCCCGGATTTTAAACATACTACTGGAAGCTAATCCTGAAGTAGTTAGTATGCTTAACTGTAAAGAGAAAATCAGTGCACTTTCTCTGGCATGTAGCTATGGTCATGTTGATGTGGTGAGGCTCTTACTGAAAGAGCCTGCGAAGCTGGGTTTAGGGAAAGAAGGGTTTGATCAAGCATGTATACACTTGGCTGTGACCGGTGGACACAAGGATGTTGTTAAAGAGTTGCTAAGAGTGTGTCCTGACCTTGCTTATATGGTTGATGAGAAAGGAAACTATCCATTGCACTATGCAACTAGCAAAGAAAACAGGGAGATAACCTGGACACTCCTCAAGCTTGATCCAAAGCTTGCTCAGCAGCACAATCTCAGTGGTCACACACCCTTGCACTTGGCAGCAATAAACTACAAAATCTCAGTCCTTCAGGTTTTTGTCTCAATGGCTAAACCATCTTTTCAGATCGTTACAAAAGCCGGAGAGACAGTGTTCCACTTGGCTGTGAAATATGGGCGCTATGATGCTATAGTATACTTGACCTGTGTCTGTGATGATGTGGATTTCTTTGACTGTCGAGACCGGTATAATAATACCATCCTACATCTTGCAGTTTCTGAAGCACAACATAAGATAGCAGAGTACCTAATCAGCAAAAAAAAAGTGGAGCTCAATTCTAGAAACAATGAAGGACTGACAGCCCTTGACCTCCTCAAACAGAGCAAGGAAAGTGAAGACAATCAGCGTCTTGAAGCCATGCTAATGAAAGCAGGAGGTAAAACAAGGATTGAACTGTTATCTACTTCACCAAAAGCAGATAAAGCCACTACTCAGCCAGTGACACTTCCTGAAAACATTCCAAAACGAGCACAATTTTTTAAAGAGCATGAACTGCAGTTGTCCATAGCTTGGTCTGCTGCATCCAAGTCCCCATCAAGATCATCAGCACCTATGTCCCAGATGTCAACACCTTTTCCATCACCCCAATCCAGCAAGTCTTCACCTAAGCTGCCATCACCGCAATCTAGCAAGTCATCGCCTAAGCTGCCATCACCACCATCTAGCATGTCATCGCCTCAGCTTCAGGTCGGGGAAGGCTCATCACGTCAGCTGCAAGTTGGATCAGCATTTGAAGTTAAAGAGGGACTGGATTATGAAAGACAGAAGAAGCATGAGTTTCTTTTGCAAACCTTTGCGAGACAACACTATCCAAGGCTTCCAAGCAAATGGAACCAAACAGAAATGAGTACTGAGACTTTCTACAACCGGCGAAGGAAACATGAAAAAGTATATACAGAGGCACTACAGAATGCAAGGAACAGTATAACACTAGTTGCCATTTTGATCGCAACGGTTACTTTTGCTGCTGGCATTAGTCCCCCGGGTGGTGTTTATCAGGATAAACATATGAAAGGGAAGTCAATAGCAGGCACAACAACTGCTTTCAAGGTGTTTGAAATAAGCAATTATGTCGCTCTATTCACATCACTCTCGATAGTTGTTGTTCTTATGAGTATCATTCCATATCGGAGAAAGCCATTGATAAGGTTGTTGAAGGTTGCTCATAAGGTACTGTGGGTAGCTGTGGCATTCATGGCAACAGGGTTTGTTGCAGCCACATGGGTTATCATGCCCCATTATCCAAAAAATGAATTGTTGTTTGTAGCCTTTTTTACTATAAGCATTGGAATTCTGGGAACAGTTTTTATTTGGCTAGGTGTTATGCTAGTTGTTCATTGCCTACGAAAGTCGAAGTGGAAGAAGAGAAGAAGAGAAATTGGGGAGGCTGATGAGGACCCTGAAATGGGAAGCCAAAACTCGGATGTTAATAGCACCTATAATCAAGGATATCATTCATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

701

Amino Acids

78.39

Weight (kDa)

8.99

Isoelectric Point (pI)

51.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 31 - 96 1.5e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 40 - 128 1.8e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 102 - 159 8.1e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 109 - 196 2e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 203 - 261 6.7e-06 Ankyrin repeats (3 copies)
PGG PF13962 513 - 622 1.5e-30 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10340 AT1G10340 AT1G34046 AT1G34050 AT2G24600 AT2G24600 AT2G24600 AT2G24600 AT3G52830 AT5G54700 AT5G54700
fragaria_vesca FvH4_6g32091 FvH4_6g32100 FvH4_6g32100 FvH4_6g32121
malus_domestica MD17G1186800.v1.1 MD17G1187000.v1.1
prunus_persica Prupe.3G061900_v2.0.a1 Prupe.3G062300_v2.0.a1 Prupe.3G062300_v2.0.a1 Prupe.3G062300_v2.0.a1 Prupe.3G062400_v2.0.a1 Prupe.3G065300_v2.0.a1
pyrus_communis pycom17g19590
rosa_chinensis RchiOBHm_Chr1g0353591 RchiOBHm_Chr2g0139761 RchiOBHm_Chr2g0139771 RchiOBHm_Chr2g0139841 RchiOBHm_Chr2g0139851 RchiOBHm_Chr2g0139911
rosa_laevigata RLG00000019831 RLG00000019832 RLG00000019834 RLG00000019835 RLG00000019838 RLG00000028268 RLG00000029493 RLG00000029500 RLG00000029519 RLG00000029774
rosa_multiflora Rmu_co8197386.1_g000001 Rmu_co8359109.1_g000001 Rmu_sc0000545.1_g000005 Rmu_sc0005914.1_g000001 Rmu_sc0030896.1_g000002 Rmu_sc0030896.1_g000003 Rmu_sc0030896.1_g000004 Rmu_sc0032265.1_g000001
rosa_roxburghii Rroxscaffold_2G00105170 Rroxscaffold_2G00105210 Rroxscaffold_2G00105220 Rroxscaffold_4G00301800
rosa_rugosa Rorug01G0236400 Rorug02G0359900 Rorug02G0360000 Rorug02G0360200
rosa_samantha Rh1AG248700 Rh1BG105500 Rh1BG219100 Rh1CG231800 Rh1DG245500 Rh2AG409600 Rh2AG409700 Rh2AG410000 Rh2AG410100 Rh2AG410400 Rh2AG410500 Rh2BG420100 Rh2BG420200 Rh2BG420600 Rh2BG420700 Rh2CG395900 Rh2CG396000 Rh2CG396200 Rh2CG396500 Rh2CG396600 Rh2DG429400 Rh2DG429500 Rh2DG429800 Rh2DG430100 Rh2DG430200
rosa_wichuraiana Rw1G021690 Rw2G033350 Rw2G033400 Rw2G033410 Rw2G033570 Rw2G033620 Rw2G033630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1149
AccI GTMKAC 2 cut(s) 427, 774
AciI CCGC 1 cut(s) 1259
AclWI GGATC 2 cut(s) 581, 1369
AcsI RAATTY 2 cut(s) 78, 1929
AcuI CTGAAG 4 cut(s) 303, 653, 879, 1307
AdeI CACNNNGTG 1 cut(s) 127
AfaI GTAC 3 cut(s) 884, 1489, 1812
AfiI CCNNNNNNNGG 7 cut(s) 151, 184, 1149, 1625, 2044, 2045, 2059
AgeI ACCGGT 2 cut(s) 443, 822
AgsI TTSAA 7 cut(s) 149, 992, 1031, 1373, 1687, 1697, 1795
AhlI ACTAGT 1 cut(s) 1570
AjiI CACGTC 1 cut(s) 1346
AjnI CCWGG 2 cut(s) 207, 566
AloI GAACNNNNNNTCC 2 cut(s) 229, 261
Alw21I GWGCWC 3 cut(s) 325, 912, 1111
Alw26I GTCTC 6 cut(s) 206, 685, 719, 813, 1432, 1487
Alw44I GTGCAC 1 cut(s) 321
AlwI GGATC 2 cut(s) 581, 1369
AlwNI CAGNNNCTG 1 cut(s) 857
Ama87I CYCGRG 2 cut(s) 83, 1621
ApaLI GTGCAC 1 cut(s) 321
ApeKI GCWGC 9 cut(s) 191, 604, 641, 1157, 1249, 1285, 1351, 1604, 1853
ApoI RAATTY 2 cut(s) 78, 1929
ArsI GACNNNNNNTTYG 2 cut(s) 648, 680
AsiGI ACCGGT 2 cut(s) 443, 822
Asp700I GAANNNNTTC 2 cut(s) 78, 1095
AspLEI GCGC 1 cut(s) 759
AspS9I GGNCC 1 cut(s) 2035
AsuC2I CCSGG 4 cut(s) 179, 250, 1622, 1623
AsuHPI GGTGA 6 cut(s) 373, 1038, 1212, 1233, 1248, 1284
AsuNHI GCTAGC 1 cut(s) 53
AvaI CYCGRG 2 cut(s) 83, 1621
AvaII GGWCC 1 cut(s) 2035
BaeGI GKGCMC 2 cut(s) 179, 325
BanII GRGCYC 1 cut(s) 912
BbsI GAAGAC 2 cut(s) 981, 1230
Bbv12I GWGCWC 3 cut(s) 325, 912, 1111
BbvCI CCTCAGC 1 cut(s) 1317
BbvI GCAGC 9 cut(s) 178, 616, 653, 1144, 1236, 1272, 1338, 1591, 1865
BccI CCATC 7 cut(s) 703, 845, 1180, 1225, 1261, 1297, 1306
BcgI CGANNNNNNTGC 2 cut(s) 1693, 1727
BciT130I CCWGG 2 cut(s) 209, 568
BclI TGATCA 1 cut(s) 415
BcnI CCSGG 4 cut(s) 179, 250, 1622, 1623
BcoDI GTCTC 6 cut(s) 206, 685, 719, 813, 1432, 1487
BcuI ACTAGT 1 cut(s) 1570
BfaI CTAG 8 cut(s) 54, 543, 918, 1266, 1302, 1571, 1955, 1967
BfmI CTRYAG 3 cut(s) 768, 1133, 1544
BfoI RGCGCY 1 cut(s) 760
BisI GCNGC 9 cut(s) 192, 605, 642, 1158, 1250, 1286, 1352, 1605, 1854
BlpI GCTNAGC 1 cut(s) 600
BlsI GCNGC 9 cut(s) 193, 606, 643, 1159, 1251, 1287, 1353, 1606, 1855
BmcAI AGTACT 1 cut(s) 1489
Bme1390I CCNGG 6 cut(s) 179, 209, 250, 568, 1622, 1623
Bme18I GGWCC 1 cut(s) 2035
BmeT110I CYCGRG 2 cut(s) 83, 1621
BmgBI CACGTC 1 cut(s) 1346
BmgT120I GGNCC 1 cut(s) 2035
BmiI GGNNCC 2 cut(s) 1472, 2037
BmrFI CCNGG 6 cut(s) 179, 209, 250, 568, 1622, 1623
BmrI ACTGGG 1 cut(s) 212
BmsI GCATC 4 cut(s) 131, 164, 754, 1169
BmtI GCTAGC 1 cut(s) 57
BmuI ACTGGG 1 cut(s) 212
BoxI GACNNNNGTC 2 cut(s) 218, 785
BpiI GAAGAC 2 cut(s) 981, 1230
Bpu10I CCTNAGC 3 cut(s) 1245, 1281, 1317
Bpu1102I GCTNAGC 1 cut(s) 600
BpuEI CTTGAG 1 cut(s) 563
BpuMI CCSGG 4 cut(s) 179, 250, 1622, 1623
BsaI GGTCTC 2 cut(s) 206, 813
BsaJI CCNNGG 4 cut(s) 248, 1451, 1620, 1621
BsaWI WCCGGW 2 cut(s) 443, 822
BsaXI ACNNNNNCTCC 2 cut(s) 714, 744
Bsc4I CCNNNNNNNGG 7 cut(s) 151, 184, 1149, 1625, 2044, 2045, 2059
Bse118I RCCGGY 3 cut(s) 443, 822, 1506
Bse1I ACTGG 4 cut(s) 218, 273, 1076, 1392
Bse3DI GCAATG 2 cut(s) 527, 1975
BseBI CCWGG 2 cut(s) 209, 568
BseDI CCNNGG 4 cut(s) 248, 1451, 1620, 1621
BseGI GGATG 5 cut(s) 146, 463, 837, 1160, 2068
BseLI CCNNNNNNNGG 7 cut(s) 151, 184, 1149, 1625, 2044, 2045, 2059
BseMI GCAATG 2 cut(s) 527, 1975
BseMII CTCAG 7 cut(s) 186, 614, 628, 675, 1085, 1331, 1482
BseNI ACTGG 4 cut(s) 218, 273, 1076, 1392
BseRI GAGGAG 2 cut(s) 566, 941
BseSI GKGCMC 2 cut(s) 179, 325
BseXI GCAGC 9 cut(s) 178, 616, 653, 1144, 1236, 1272, 1338, 1591, 1865
BseYI CCCAGC 1 cut(s) 391
BshTI ACCGGT 2 cut(s) 443, 822
BsiHKAI GWGCWC 3 cut(s) 325, 912, 1111
BsiHKCI CYCGRG 2 cut(s) 83, 1621
BsiSI CCGG 7 cut(s) 179, 250, 444, 720, 823, 1507, 1622
BslFI GGGAC 4 cut(s) 1153, 1180, 1398, 1602
BslI CCNNNNNNNGG 7 cut(s) 151, 184, 1149, 1625, 2044, 2045, 2059
BsmAI GTCTC 6 cut(s) 206, 685, 719, 813, 1432, 1487
BsmFI GGGAC 4 cut(s) 1153, 1180, 1398, 1602
BsmI GAATGC 2 cut(s) 1555, 1829
Bso31I GGTCTC 2 cut(s) 206, 813
BsoBI CYCGRG 2 cut(s) 83, 1621
Bsp1286I GDGCHC 4 cut(s) 179, 325, 912, 1111
Bsp143I GATC 6 cut(s) 415, 586, 705, 1178, 1361, 1584
Bsp1720I GCTNAGC 1 cut(s) 600
BspACI CCGC 1 cut(s) 1259
BspCNI CTCAG 7 cut(s) 187, 613, 627, 674, 1084, 1330, 1483
BspLI GGNNCC 2 cut(s) 1472, 2037
BspMAI CTGCAG 1 cut(s) 1137
BspOI GCTAGC 1 cut(s) 57
BspPI GGATC 2 cut(s) 581, 1369
BspTNI GGTCTC 2 cut(s) 206, 813
BsrDI GCAATG 2 cut(s) 527, 1975
BsrFI RCCGGY 3 cut(s) 443, 822, 1506
BsrI ACTGG 4 cut(s) 218, 273, 1076, 1392
BssAI RCCGGY 3 cut(s) 443, 822, 1506
BssECI CCNNGG 4 cut(s) 248, 1451, 1620, 1621
BssMI GATC 6 cut(s) 415, 586, 705, 1178, 1361, 1584
BssNAI GTATAC 2 cut(s) 428, 775
BssT1I CCWWGG 1 cut(s) 1451
Bst1107I GTATAC 2 cut(s) 428, 775
Bst2UI CCWGG 2 cut(s) 209, 568
Bst4CI ACNGT 8 cut(s) 305, 730, 815, 1035, 1562, 1594, 1815, 1942
Bst6I CTCTTC 2 cut(s) 1997, 2005
BstAPI GCANNNNNTGC 1 cut(s) 1132
BstC8I GCNNGC 5 cut(s) 55, 384, 597, 1609, 1669
BstF5I GGATG 5 cut(s) 146, 463, 837, 1160, 2068
BstH2I RGCGCY 1 cut(s) 760
BstHHI GCGC 1 cut(s) 759
BstKTI GATC 6 cut(s) 418, 589, 708, 1181, 1364, 1587
BstMAI GTCTC 6 cut(s) 206, 685, 719, 813, 1432, 1487
BstMBI GATC 6 cut(s) 415, 586, 705, 1178, 1361, 1584
BstMWI GCNNNNNNNGC 8 cut(s) 388, 601, 1061, 1132, 1154, 1258, 1285, 1835
BstNI CCWGG 2 cut(s) 209, 568
BstNSI RCATGY 3 cut(s) 339, 426, 1309
BstPAI GACNNNNGTC 2 cut(s) 218, 785
BstSCI CCNGG 6 cut(s) 177, 207, 248, 566, 1620, 1621
BstSFI CTRYAG 3 cut(s) 768, 1133, 1544
BstSLI GKGCMC 2 cut(s) 179, 325
BstV1I GCAGC 9 cut(s) 178, 616, 653, 1144, 1236, 1272, 1338, 1591, 1865
BstV2I GAAGAC 2 cut(s) 981, 1230
BstXI CCANNNNNNTGG 1 cut(s) 1467
BstZ17I GTATAC 2 cut(s) 428, 775
BtgZI GCGATG 2 cut(s) 1260, 1296
BtrI CACGTC 1 cut(s) 1346
BtsCI GGATG 5 cut(s) 146, 463, 837, 1160, 2068
BtsIMutI CAGTG 4 cut(s) 325, 622, 735, 1083
Cac8I GCNNGC 5 cut(s) 55, 384, 597, 1609, 1669
CaiI CAGNNNCTG 1 cut(s) 857
CfoI GCGC 1 cut(s) 759
Cfr10I RCCGGY 3 cut(s) 443, 822, 1506
Cfr13I GGNCC 1 cut(s) 2035
Cfr9I CCCGGG 1 cut(s) 1621
CseI GACGC 1 cut(s) 974
Csp6I GTAC 3 cut(s) 883, 1488, 1811
CspAI ACCGGT 2 cut(s) 443, 822
CspCI CAANNNNNGTGG 2 cut(s) 598, 633
CviQI GTAC 3 cut(s) 883, 1488, 1811
DpnI GATC 6 cut(s) 417, 588, 707, 1180, 1363, 1586
DpnII GATC 6 cut(s) 415, 586, 705, 1178, 1361, 1584
DraI TTTAAA 2 cut(s) 258, 1120
DraIII CACNNNGTG 1 cut(s) 127
Eam1104I CTCTTC 2 cut(s) 1997, 2005
EarI CTCTTC 2 cut(s) 1997, 2005
Ecl136II GAGCTC 1 cut(s) 910
Eco130I CCWWGG 1 cut(s) 1451
Eco24I GRGCYC 1 cut(s) 912
Eco31I GGTCTC 2 cut(s) 206, 813
Eco32I GATATC 1 cut(s) 2090
Eco47I GGWCC 1 cut(s) 2035
Eco53kI GAGCTC 1 cut(s) 910
Eco57I CTGAAG 4 cut(s) 303, 653, 879, 1307
Eco88I CYCGRG 2 cut(s) 83, 1621
EcoICRI GAGCTC 1 cut(s) 910
EcoO109I RGGNCCY 1 cut(s) 2035
EcoRI GAATTC 2 cut(s) 78, 1929
EcoRII CCWGG 2 cut(s) 207, 566
EcoRV GATATC 1 cut(s) 2090
EcoT14I CCWWGG 1 cut(s) 1451
EcoT38I GRGCYC 1 cut(s) 912
ErhI CCWWGG 1 cut(s) 1451
FaqI GGGAC 4 cut(s) 1153, 1180, 1398, 1602
FauNDI CATATG 1 cut(s) 1647
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 2 cut(s) 427, 774
Fnu4HI GCNGC 9 cut(s) 192, 605, 642, 1158, 1250, 1286, 1352, 1605, 1854
FokI GGATG 5 cut(s) 153, 470, 824, 1147, 2075
FriOI GRGCYC 1 cut(s) 912
Fsp4HI GCNGC 9 cut(s) 192, 605, 642, 1158, 1250, 1286, 1352, 1605, 1854
FspBI CTAG 8 cut(s) 54, 543, 918, 1266, 1302, 1571, 1955, 1967
GlaI GCGC 1 cut(s) 758
GluI GCNGC 9 cut(s) 192, 605, 642, 1158, 1250, 1286, 1352, 1605, 1854
GsaI CCCAGC 1 cut(s) 395
HaeII RGCGCY 1 cut(s) 760
HapII CCGG 7 cut(s) 179, 250, 444, 720, 823, 1507, 1622
HgaI GACGC 1 cut(s) 974
HhaI GCGC 1 cut(s) 759
Hin6I GCGC 1 cut(s) 757
HinP1I GCGC 1 cut(s) 757
HincII GTYRAC 1 cut(s) 1206
HindII GTYRAC 1 cut(s) 1206
HindIII AAGCTT 3 cut(s) 228, 581, 593
HinfI GANTC 1 cut(s) 244
HpaII CCGG 7 cut(s) 179, 250, 444, 720, 823, 1507, 1622
HphI GGTGA 6 cut(s) 373, 1038, 1212, 1233, 1248, 1284
Hpy166II GTNNAC 5 cut(s) 323, 428, 449, 775, 1206
Hpy188I TCNGA 7 cut(s) 65, 108, 160, 705, 859, 1770, 2062
Hpy8I GTNNAC 5 cut(s) 323, 428, 449, 775, 1206
HpyAV CCTTC 7 cut(s) 68, 401, 677, 923, 1328, 1506, 1789
HpyCH4III ACNGT 8 cut(s) 305, 730, 815, 1035, 1562, 1594, 1815, 1942
HpyCH4IV ACGT 1 cut(s) 1345
HpyF10VI GCNNNNNNNGC 8 cut(s) 388, 601, 1061, 1132, 1154, 1258, 1285, 1835
HpySE526I ACGT 1 cut(s) 1345
HspAI GCGC 1 cut(s) 757
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 6 cut(s) 415, 586, 705, 1178, 1361, 1584
LmnI GCTCC 1 cut(s) 907
Lsp1109I GCAGC 9 cut(s) 178, 616, 653, 1144, 1236, 1272, 1338, 1591, 1865
LweI GCATC 4 cut(s) 131, 164, 754, 1169
MaeI CTAG 8 cut(s) 54, 543, 918, 1266, 1302, 1571, 1955, 1967
MaeII ACGT 1 cut(s) 1345
MaeIII GTNAC 6 cut(s) 43, 439, 620, 709, 1078, 1594
MalI GATC 6 cut(s) 417, 588, 707, 1180, 1363, 1586
MboI GATC 6 cut(s) 415, 586, 705, 1178, 1361, 1584
MboII GAAGA 8 cut(s) 107, 986, 1230, 1417, 2011, 2014, 2019, 2022
MfeI CAATTG 1 cut(s) 24
MhlI GDGCHC 4 cut(s) 179, 325, 912, 1111
MluCI AATT 9 cut(s) 24, 78, 162, 913, 1112, 1705, 1892, 1929, 2016
MmeI TCCRAC 1 cut(s) 1339
MroXI GAANNNNTTC 2 cut(s) 78, 1095
MseI TTAA 7 cut(s) 257, 300, 465, 1119, 1377, 2067, 2101
MslI CAYNNNNRTG 3 cut(s) 354, 456, 1001
MspA1I CMGCKG 1 cut(s) 1351
MspI CCGG 7 cut(s) 179, 250, 444, 720, 823, 1507, 1622
MspR9I CCNGG 6 cut(s) 179, 209, 250, 568, 1622, 1623
MunI CAATTG 1 cut(s) 24
Mva1269I GAATGC 2 cut(s) 1555, 1829
MvaI CCWGG 2 cut(s) 209, 568
MwoI GCNNNNNNNGC 8 cut(s) 388, 601, 1061, 1132, 1154, 1258, 1285, 1835
NciI CCSGG 4 cut(s) 179, 250, 1622, 1623
NdeI CATATG 1 cut(s) 1647
NdeII GATC 6 cut(s) 415, 586, 705, 1178, 1361, 1584
NheI GCTAGC 1 cut(s) 53
NlaIV GGNNCC 2 cut(s) 1472, 2037
NmuCI GTSAC 4 cut(s) 43, 439, 620, 1078
NspI RCATGY 3 cut(s) 339, 426, 1309
PaeR7I CTCGAG 1 cut(s) 83
PctI GAATGC 2 cut(s) 1555, 1829
PdmI GAANNNNTTC 2 cut(s) 78, 1095
PfeI GAWTC 1 cut(s) 244
PflFI GACNNNGTC 1 cut(s) 41
PflMI CCANNNNNTGG 1 cut(s) 1149
PinAI ACCGGT 2 cut(s) 443, 822
PkrI GCNGC 9 cut(s) 193, 606, 643, 1159, 1251, 1287, 1353, 1606, 1855
PpuMI RGGWCCY 1 cut(s) 2035
PshAI GACNNNNGTC 2 cut(s) 218, 785
Psp124BI GAGCTC 1 cut(s) 912
Psp5II RGGWCCY 1 cut(s) 2035
Psp6I CCWGG 2 cut(s) 207, 566
PspFI CCCAGC 1 cut(s) 391
PspGI CCWGG 2 cut(s) 207, 566
PspN4I GGNNCC 2 cut(s) 1472, 2037
PspPI GGNCC 1 cut(s) 2035
PspPPI RGGWCCY 1 cut(s) 2035
PstI CTGCAG 1 cut(s) 1137
PstNI CAGNNNCTG 1 cut(s) 857
PsyI GACNNNGTC 1 cut(s) 41
PvuII CAGCTG 1 cut(s) 1351
RsaI GTAC 3 cut(s) 884, 1489, 1812
RsaNI GTAC 3 cut(s) 883, 1488, 1811
RseI CAYNNNNRTG 3 cut(s) 354, 456, 1001
SacI GAGCTC 1 cut(s) 912
SaqAI TTAA 7 cut(s) 257, 300, 465, 1119, 1377, 2067, 2101
SatI GCNGC 9 cut(s) 192, 605, 642, 1158, 1250, 1286, 1352, 1605, 1854
Sau3AI GATC 6 cut(s) 415, 586, 705, 1178, 1361, 1584
Sau96I GGNCC 1 cut(s) 2035
ScaI AGTACT 1 cut(s) 1489
ScrFI CCNGG 6 cut(s) 179, 209, 250, 568, 1622, 1623
SduI GDGCHC 4 cut(s) 179, 325, 912, 1111
SfaNI GCATC 4 cut(s) 131, 164, 754, 1169
SfcI CTRYAG 3 cut(s) 768, 1133, 1544
Sfr274I CTCGAG 1 cut(s) 83
SinI GGWCC 1 cut(s) 2035
SlaI CTCGAG 1 cut(s) 83
SmaI CCCGGG 1 cut(s) 1623
SmiMI CAYNNNNRTG 3 cut(s) 354, 456, 1001
SmlI CTYRAG 2 cut(s) 83, 578
SmoI CTYRAG 2 cut(s) 83, 578
SpeI ACTAGT 1 cut(s) 1570
Sse9I AATT 9 cut(s) 24, 78, 162, 913, 1112, 1705, 1892, 1929, 2016
SsiI CCGC 1 cut(s) 1259
SspMI CTAG 8 cut(s) 54, 543, 918, 1266, 1302, 1571, 1955, 1967
SstI GAGCTC 1 cut(s) 912
StyD4I CCNGG 6 cut(s) 177, 207, 248, 566, 1620, 1621
StyI CCWWGG 1 cut(s) 1451
TaaI ACNGT 8 cut(s) 305, 730, 815, 1035, 1562, 1594, 1815, 1942
TaiI ACGT 1 cut(s) 1348
TaqI TCGA 5 cut(s) 84, 235, 817, 1733, 1991
TasI AATT 9 cut(s) 24, 78, 162, 913, 1112, 1705, 1892, 1929, 2016
TatI WGTACW 1 cut(s) 1487
TfiI GAWTC 1 cut(s) 244
Tru1I TTAA 7 cut(s) 257, 300, 465, 1119, 1377, 2067, 2101
Tru9I TTAA 7 cut(s) 257, 300, 465, 1119, 1377, 2067, 2101
TscAI CASTG 4 cut(s) 325, 622, 735, 1083
TseFI GTSAC 4 cut(s) 43, 439, 620, 1078
TseI GCWGC 9 cut(s) 191, 604, 641, 1157, 1249, 1285, 1351, 1604, 1853
Tsp45I GTSAC 4 cut(s) 43, 439, 620, 1078
TspMI CCCGGG 1 cut(s) 1621
TspRI CASTG 4 cut(s) 325, 622, 735, 1083
Tth111I GACNNNGTC 1 cut(s) 41
Van91I CCANNNNNTGG 1 cut(s) 1149
VneI GTGCAC 1 cut(s) 321
VpaK11BI GGWCC 1 cut(s) 2035
XapI RAATTY 2 cut(s) 78, 1929
XbaI TCTAGA 1 cut(s) 917
XceI RCATGY 3 cut(s) 339, 426, 1309
XhoI CTCGAG 1 cut(s) 83
XmaI CCCGGG 1 cut(s) 1621
XmiI GTMKAC 2 cut(s) 427, 774
XmnI GAANNNNTTC 2 cut(s) 78, 1095
XspI CTAG 8 cut(s) 54, 543, 918, 1266, 1302, 1571, 1955, 1967
ZrmI AGTACT 1 cut(s) 1489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.