Rroxscaffold_4G00301800

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000496
Physical Location & Seq
Reverse (-)
22128502 .. 22131246
2745 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301800.1

Sequence Viewer

Length: 2094 bp
ATGGATTTCAGGATTTTAGAGGCAATCGAGAGAAAGGACATAGTCACATTTGCTAGCTTAGTCCGAGAAAATGAAGGAATTCTCGAGCATAGAGAAGCTGATACTCTGAACACGCCATTACACCTTGTGTCAAAGTTTGGATGCCTTGAAATGGCATCTGAAATTGTTTCTTTGTGCCCAGACATGGTTGCTGCTGAGAATAAGAACAAGGAGACTCCAGTCCATGAAGCTTGTCGAACAGGAAATCCCCGGATTTTAAACATCCTACTGGAAGCTAATCCTGAAGTAGCTAGTAAGCTTAACAGTAAAGAAAAAATCAGTGCACTTTCTCTGGCATGTAGCTATGGTCATCTTGATGTGGTGAGGCTCTTACTGAAAGAGCCTGGGAAGCTGGGTTTAGGGAAAGAAGGGTTTGATCAAGCGTGTATACACTTGGCTGTGACTGGTGGACACAAGGATATTGTTAAAGAGTTGCTAAGAGTGTGTCCTGACCTTGCTTATATGGTTGATGAGAAAGGAAACTATCCATTGCACTATGCAACTAGCAAAGAAAACAGGGAGATAACCTGGACACTCCTCAAGCTTGATCCAAAGCTTGCTCAGCAGCGCAATCTCAGTGGTCACACACCCTTGCACTTGGCAGCAATAAACTACAAAATCTCAGTCCTTCAGGTTTTTGTCTCAATGGCTAAACCATCTTTTCAGATAGTTACAAAAGCCGGAGAGACAGTGTTCCACTTGGCTGTGAAATATGGGCGCTATGATGCTATTGTATACTTGACCTGTGTCTGTGATGATGTGGATTTCTTTGACTGTCGAGACTGTTATAGTAATACCATCCTACATCTTGCAGTTTCTGAAGCACAACATAAGATAGCAGAGTACCTAATCAGCAAAAAAAAAGTGGAGCTCAATTCTAGAAACAATGAAGGACTGACAGCCCTTGACCTCCTCAAACAGAGCAAGGAAAGTGAAGACAATCAGCGTCTTGAAGCCATGCTAATGAAAGCAGGAGGTAAAACAAGGATTGAACTGTTATCTACTTCACCAAAAGCAGATAAAGCCACTGCTCAGCCAGTGACACTTCCTGAAAACATTCCAAAACGAGCACAATTTTTTAAAGAGCATGAACTGCAGTTGTCCATAGCTTGGTCTGCTGCATCCAAGTCCCCATCACGATCATCAGCACCTATGTCCCAGATGTCAACACCTTTTCCATCACCCCAATCCAGCAAGTCTTCACCTAAGCTGCCATCACCGCAATCTAGCAAGTCATCGCCTAAGCTGCCATCCCCACCATCTAGCAAGTCATCGCCTCAGCTTCAGGTTGGGGAAGGCTCATTACGTCATCAGCAAGTTGGATCAGCATTTGAAGTTAAAGAGGGACTGGAATATGAAAGACAGAAGAAGCATGAGTTTCTTTTGCAAACTTTTGCGAGACAACACTATCCAAGCAAATGGAACCAAACAGAAACGAGTACTGAGATTTTCTACAGCCGGCGAAGGAAACATGAAAAAGTATATACAGAGGCACTACAGAATGCAAGGAACACTATAACGCTAGTTGCCATTTTGATCGCAACGGTTACTTTTGCTGCTGGCATTAGTCCCCCGGGTGGTGTTTATCAGGATAAACATATGAAAGGGAAGTCAATAGCAGGCACAACAACTGCTTTCAAGGTGTTTGAAATAAGCAATTATGTCGCGCTATTCACATCACTCTCGATAGTTGTTGTTCTTATGAGTATCATTCCATATCGGAGAAAGCCATTGATAAGGTTGTTGAGGGTTGCTCATAAGGTACTGTGGGTAGCTGTGGCATTCATGGCAACAGGGTTTGTTGCAGCCACATGGGTTATCATGCCACATAATCCGAAAAATGAATTGTTGTTTGTAGCCTTTTTGACTATAAGCATTGGAATTCTGGGAACAGTTTTTATTTGGCTAGGTGTTATGCTAGTTGTTCATTGCCTACGAAAGTCGAAGTGGAAGAAGAGAAGAAGAGAAATTGGGGTGGCTGATGAGGACCCTGAAATGGGAAGCCAAAACTCAGATGTTCATAGCACCTATAATCAAGGATATCATTCATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

697

Amino Acids

77.83

Weight (kDa)

9.16

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 34 - 96 2.4e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 40 - 127 1.4e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 100 - 159 1.1e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 109 - 196 1.3e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 204 - 261 7.1e-06 Ankyrin repeats (3 copies)
PGG PF13962 510 - 619 9.2e-31 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10340 AT1G10340 AT1G34046 AT1G34050 AT2G24600 AT2G24600 AT2G24600 AT2G24600 AT3G52830 AT5G54700 AT5G54700
fragaria_vesca FvH4_6g32091 FvH4_6g32100 FvH4_6g32100 FvH4_6g32121
malus_domestica MD17G1186800.v1.1 MD17G1187000.v1.1
prunus_persica Prupe.3G061900_v2.0.a1 Prupe.3G062300_v2.0.a1 Prupe.3G062300_v2.0.a1 Prupe.3G062300_v2.0.a1 Prupe.3G062400_v2.0.a1 Prupe.3G065300_v2.0.a1
pyrus_communis pycom17g19590
rosa_chinensis RchiOBHm_Chr1g0353591 RchiOBHm_Chr2g0139761 RchiOBHm_Chr2g0139771 RchiOBHm_Chr2g0139841 RchiOBHm_Chr2g0139851 RchiOBHm_Chr2g0139911
rosa_laevigata RLG00000019831 RLG00000019832 RLG00000019834 RLG00000019835 RLG00000019838 RLG00000028268 RLG00000029493 RLG00000029500 RLG00000029519 RLG00000029774
rosa_multiflora Rmu_co8197386.1_g000001 Rmu_co8359109.1_g000001 Rmu_sc0000545.1_g000005 Rmu_sc0005914.1_g000001 Rmu_sc0030896.1_g000002 Rmu_sc0030896.1_g000003 Rmu_sc0030896.1_g000004 Rmu_sc0032265.1_g000001
rosa_roxburghii Rroxscaffold_2G00105170 Rroxscaffold_2G00105210 Rroxscaffold_2G00105220 Rroxscaffold_4G00301800
rosa_rugosa Rorug01G0236400 Rorug02G0359900 Rorug02G0360000 Rorug02G0360200
rosa_samantha Rh1AG248700 Rh1BG105500 Rh1BG219100 Rh1CG231800 Rh1DG245500 Rh2AG409600 Rh2AG409700 Rh2AG410000 Rh2AG410100 Rh2AG410400 Rh2AG410500 Rh2BG420100 Rh2BG420200 Rh2BG420600 Rh2BG420700 Rh2CG395900 Rh2CG396000 Rh2CG396200 Rh2CG396500 Rh2CG396600 Rh2DG429400 Rh2DG429500 Rh2DG429800 Rh2DG430100 Rh2DG430200
rosa_wichuraiana Rw1G021690 Rw2G033350 Rw2G033400 Rw2G033410 Rw2G033570 Rw2G033620 Rw2G033630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1149
AccI GTMKAC 2 cut(s) 427, 774
AccII CGCG 1 cut(s) 1706
AciI CCGC 1 cut(s) 1259
AclWI GGATC 2 cut(s) 581, 1369
AcsI RAATTY 2 cut(s) 78, 1920
AcuI CTGAAG 4 cut(s) 303, 653, 879, 1307
AdeI CACNNNGTG 1 cut(s) 127
AfaI GTAC 3 cut(s) 884, 1480, 1803
AfiI CCNNNNNNNGG 6 cut(s) 151, 184, 1149, 1616, 2035, 2036
AgsI TTSAA 6 cut(s) 149, 992, 1031, 1373, 1678, 1688
AjnI CCWGG 2 cut(s) 382, 566
AloI GAACNNNNNNTCC 2 cut(s) 229, 261
Alw21I GWGCWC 3 cut(s) 325, 912, 1111
Alw26I GTCTC 5 cut(s) 206, 685, 719, 813, 1432
Alw44I GTGCAC 1 cut(s) 321
AlwI GGATC 2 cut(s) 581, 1369
AlwNI CAGNNNCTG 1 cut(s) 857
Ama87I CYCGRG 2 cut(s) 83, 1612
ApaLI GTGCAC 1 cut(s) 321
ApeKI GCWGC 8 cut(s) 191, 604, 641, 1157, 1249, 1285, 1595, 1844
ApoI RAATTY 2 cut(s) 78, 1920
ArsI GACNNNNNNTTYG 2 cut(s) 648, 680
Asp700I GAANNNNTTC 2 cut(s) 78, 1095
AspLEI GCGC 3 cut(s) 609, 759, 1708
AspS9I GGNCC 1 cut(s) 2026
AsuC2I CCSGG 3 cut(s) 250, 1613, 1614
AsuHPI GGTGA 5 cut(s) 373, 1038, 1212, 1233, 1248
AsuNHI GCTAGC 1 cut(s) 53
AvaI CYCGRG 2 cut(s) 83, 1612
AvaII GGWCC 1 cut(s) 2026
BaeGI GKGCMC 2 cut(s) 179, 325
BanII GRGCYC 1 cut(s) 912
BarI GAAGNNNNNNTAC 2 cut(s) 1326, 1358
BbsI GAAGAC 2 cut(s) 981, 1230
Bbv12I GWGCWC 3 cut(s) 325, 912, 1111
BbvCI CCTCAGC 1 cut(s) 1317
BbvI GCAGC 8 cut(s) 178, 616, 653, 1144, 1236, 1272, 1582, 1856
BccI CCATC 7 cut(s) 703, 845, 1180, 1225, 1261, 1297, 1306
BcgI CGANNNNNNTGC 2 cut(s) 1684, 1718
BciT130I CCWGG 2 cut(s) 384, 568
BclI TGATCA 1 cut(s) 415
BcnI CCSGG 3 cut(s) 250, 1613, 1614
BcoDI GTCTC 5 cut(s) 206, 685, 719, 813, 1432
BfaI CTAG 9 cut(s) 54, 291, 543, 918, 1266, 1302, 1562, 1946, 1958
BfmI CTRYAG 3 cut(s) 1133, 1492, 1535
BfoI RGCGCY 1 cut(s) 760
BisI GCNGC 8 cut(s) 192, 605, 642, 1158, 1250, 1286, 1596, 1845
BlpI GCTNAGC 2 cut(s) 600, 1071
BlsI GCNGC 8 cut(s) 193, 606, 643, 1159, 1251, 1287, 1597, 1846
BmcAI AGTACT 1 cut(s) 1480
Bme1390I CCNGG 5 cut(s) 250, 384, 568, 1613, 1614
Bme18I GGWCC 1 cut(s) 2026
BmeT110I CYCGRG 2 cut(s) 83, 1612
BmgT120I GGNCC 1 cut(s) 2026
BmiI GGNNCC 2 cut(s) 1463, 2028
BmrFI CCNGG 5 cut(s) 250, 384, 568, 1613, 1614
BmsI GCATC 4 cut(s) 131, 164, 754, 1169
BmtI GCTAGC 1 cut(s) 57
BoxI GACNNNNGTC 2 cut(s) 218, 785
BpiI GAAGAC 2 cut(s) 981, 1230
BplI GAGNNNNNCTC 2 cut(s) 1777, 1809
BpmI CTGGAG 1 cut(s) 201
Bpu10I CCTNAGC 3 cut(s) 1245, 1281, 1317
Bpu1102I GCTNAGC 2 cut(s) 600, 1071
BpuEI CTTGAG 1 cut(s) 563
BpuMI CCSGG 3 cut(s) 250, 1613, 1614
BsaJI CCNNGG 4 cut(s) 248, 383, 1611, 1612
BsaXI ACNNNNNCTCC 2 cut(s) 714, 744
Bsc4I CCNNNNNNNGG 6 cut(s) 151, 184, 1149, 1616, 2035, 2036
Bse118I RCCGGY 1 cut(s) 1497
Bse1I ACTGG 5 cut(s) 218, 273, 448, 1076, 1392
Bse3DI GCAATG 2 cut(s) 527, 1966
BseBI CCWGG 2 cut(s) 384, 568
BseDI CCNNGG 4 cut(s) 248, 383, 1611, 1612
BseGI GGATG 5 cut(s) 146, 261, 837, 1160, 1289
BseLI CCNNNNNNNGG 6 cut(s) 151, 184, 1149, 1616, 2035, 2036
BseMI GCAATG 2 cut(s) 527, 1966
BseMII CTCAG 8 cut(s) 186, 614, 628, 675, 1085, 1331, 1473, 2064
BseNI ACTGG 5 cut(s) 218, 273, 448, 1076, 1392
BseRI GAGGAG 2 cut(s) 566, 941
BseSI GKGCMC 2 cut(s) 179, 325
BseXI GCAGC 8 cut(s) 178, 616, 653, 1144, 1236, 1272, 1582, 1856
BseYI CCCAGC 1 cut(s) 391
Bsh1236I CGCG 1 cut(s) 1706
BsiHKAI GWGCWC 3 cut(s) 325, 912, 1111
BsiHKCI CYCGRG 2 cut(s) 83, 1612
BsiSI CCGG 4 cut(s) 250, 720, 1498, 1613
BslFI GGGAC 4 cut(s) 1153, 1180, 1398, 1593
BslI CCNNNNNNNGG 6 cut(s) 151, 184, 1149, 1616, 2035, 2036
BsmAI GTCTC 5 cut(s) 206, 685, 719, 813, 1432
BsmFI GGGAC 4 cut(s) 1153, 1180, 1398, 1593
BsmI GAATGC 2 cut(s) 1546, 1820
BsoBI CYCGRG 2 cut(s) 83, 1612
Bsp1286I GDGCHC 4 cut(s) 179, 325, 912, 1111
Bsp143I GATC 5 cut(s) 415, 586, 1178, 1361, 1575
Bsp1720I GCTNAGC 2 cut(s) 600, 1071
BspACI CCGC 1 cut(s) 1259
BspCNI CTCAG 8 cut(s) 187, 613, 627, 674, 1084, 1330, 1474, 2063
BspFNI CGCG 1 cut(s) 1706
BspLI GGNNCC 2 cut(s) 1463, 2028
BspMAI CTGCAG 1 cut(s) 1137
BspOI GCTAGC 1 cut(s) 57
BspPI GGATC 2 cut(s) 581, 1369
BsrDI GCAATG 2 cut(s) 527, 1966
BsrFI RCCGGY 1 cut(s) 1497
BsrI ACTGG 5 cut(s) 218, 273, 448, 1076, 1392
BssAI RCCGGY 1 cut(s) 1497
BssECI CCNNGG 4 cut(s) 248, 383, 1611, 1612
BssMI GATC 5 cut(s) 415, 586, 1178, 1361, 1575
BssNAI GTATAC 2 cut(s) 428, 775
Bst1107I GTATAC 2 cut(s) 428, 775
Bst2UI CCWGG 2 cut(s) 384, 568
Bst4CI ACNGT 8 cut(s) 305, 730, 815, 824, 1035, 1585, 1806, 1933
Bst6I CTCTTC 2 cut(s) 1988, 1996
BstAPI GCANNNNNTGC 1 cut(s) 1132
BstC8I GCNNGC 5 cut(s) 55, 597, 1499, 1600, 1660
BstF5I GGATG 5 cut(s) 146, 261, 837, 1160, 1289
BstFNI CGCG 1 cut(s) 1706
BstH2I RGCGCY 1 cut(s) 760
BstHHI GCGC 3 cut(s) 609, 759, 1708
BstKTI GATC 5 cut(s) 418, 589, 1181, 1364, 1578
BstMAI GTCTC 5 cut(s) 206, 685, 719, 813, 1432
BstMBI GATC 5 cut(s) 415, 586, 1178, 1361, 1575
BstMWI GCNNNNNNNGC 8 cut(s) 388, 601, 1061, 1132, 1154, 1258, 1285, 1826
BstNI CCWGG 2 cut(s) 384, 568
BstNSI RCATGY 1 cut(s) 339
BstPAI GACNNNNGTC 2 cut(s) 218, 785
BstSCI CCNGG 5 cut(s) 248, 382, 566, 1611, 1612
BstSFI CTRYAG 3 cut(s) 1133, 1492, 1535
BstSLI GKGCMC 2 cut(s) 179, 325
BstUI CGCG 1 cut(s) 1706
BstV1I GCAGC 8 cut(s) 178, 616, 653, 1144, 1236, 1272, 1582, 1856
BstV2I GAAGAC 2 cut(s) 981, 1230
BstXI CCANNNNNNTGG 1 cut(s) 1458
BstZ17I GTATAC 2 cut(s) 428, 775
BtgZI GCGATG 2 cut(s) 1260, 1296
BtsCI GGATG 5 cut(s) 146, 261, 837, 1160, 1289
BtsI GCAGTG 1 cut(s) 1065
BtsIMutI CAGTG 5 cut(s) 325, 622, 735, 1065, 1083
Cac8I GCNNGC 5 cut(s) 55, 597, 1499, 1600, 1660
CaiI CAGNNNCTG 1 cut(s) 857
CfoI GCGC 3 cut(s) 609, 759, 1708
Cfr10I RCCGGY 1 cut(s) 1497
Cfr13I GGNCC 1 cut(s) 2026
Cfr9I CCCGGG 1 cut(s) 1612
CseI GACGC 1 cut(s) 974
Csp6I GTAC 3 cut(s) 883, 1479, 1802
CspCI CAANNNNNGTGG 2 cut(s) 598, 633
CviQI GTAC 3 cut(s) 883, 1479, 1802
DpnI GATC 5 cut(s) 417, 588, 1180, 1363, 1577
DpnII GATC 5 cut(s) 415, 586, 1178, 1361, 1575
DraI TTTAAA 2 cut(s) 258, 1120
DraIII CACNNNGTG 1 cut(s) 127
Eam1104I CTCTTC 2 cut(s) 1988, 1996
EarI CTCTTC 2 cut(s) 1988, 1996
Ecl136II GAGCTC 1 cut(s) 910
Eco24I GRGCYC 1 cut(s) 912
Eco32I GATATC 1 cut(s) 2081
Eco47I GGWCC 1 cut(s) 2026
Eco53kI GAGCTC 1 cut(s) 910
Eco57I CTGAAG 4 cut(s) 303, 653, 879, 1307
Eco88I CYCGRG 2 cut(s) 83, 1612
EcoICRI GAGCTC 1 cut(s) 910
EcoO109I RGGNCCY 1 cut(s) 2026
EcoRI GAATTC 2 cut(s) 78, 1920
EcoRII CCWGG 2 cut(s) 382, 566
EcoRV GATATC 1 cut(s) 2081
EcoT38I GRGCYC 1 cut(s) 912
FaqI GGGAC 4 cut(s) 1153, 1180, 1398, 1593
FauNDI CATATG 1 cut(s) 1638
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 2 cut(s) 427, 774
Fnu4HI GCNGC 8 cut(s) 192, 605, 642, 1158, 1250, 1286, 1596, 1845
FokI GGATG 5 cut(s) 153, 248, 824, 1147, 1276
FriOI GRGCYC 1 cut(s) 912
Fsp4HI GCNGC 8 cut(s) 192, 605, 642, 1158, 1250, 1286, 1596, 1845
FspBI CTAG 9 cut(s) 54, 291, 543, 918, 1266, 1302, 1562, 1946, 1958
GlaI GCGC 3 cut(s) 608, 758, 1707
GluI GCNGC 8 cut(s) 192, 605, 642, 1158, 1250, 1286, 1596, 1845
GsaI CCCAGC 1 cut(s) 395
GsuI CTGGAG 1 cut(s) 201
HaeII RGCGCY 1 cut(s) 760
HapII CCGG 4 cut(s) 250, 720, 1498, 1613
HgaI GACGC 1 cut(s) 974
HhaI GCGC 3 cut(s) 609, 759, 1708
Hin6I GCGC 3 cut(s) 607, 757, 1706
HinP1I GCGC 3 cut(s) 607, 757, 1706
HincII GTYRAC 1 cut(s) 1206
HindII GTYRAC 1 cut(s) 1206
HindIII AAGCTT 4 cut(s) 228, 296, 581, 593
HinfI GANTC 1 cut(s) 214
HpaII CCGG 4 cut(s) 250, 720, 1498, 1613
HphI GGTGA 5 cut(s) 373, 1038, 1212, 1233, 1248
Hpy166II GTNNAC 5 cut(s) 323, 428, 449, 775, 1206
Hpy188I TCNGA 8 cut(s) 65, 108, 160, 705, 859, 1761, 1875, 2053
Hpy8I GTNNAC 5 cut(s) 323, 428, 449, 775, 1206
HpyAV CCTTC 6 cut(s) 68, 401, 677, 923, 1328, 1497
HpyCH4III ACNGT 8 cut(s) 305, 730, 815, 824, 1035, 1585, 1806, 1933
HpyCH4IV ACGT 1 cut(s) 1345
HpyF10VI GCNNNNNNNGC 8 cut(s) 388, 601, 1061, 1132, 1154, 1258, 1285, 1826
HpySE526I ACGT 1 cut(s) 1345
HspAI GCGC 3 cut(s) 607, 757, 1706
KroI GCCGGC 1 cut(s) 1497
KroNI GCCGGC 1 cut(s) 1499
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 5 cut(s) 415, 586, 1178, 1361, 1575
LmnI GCTCC 1 cut(s) 907
Lsp1109I GCAGC 8 cut(s) 178, 616, 653, 1144, 1236, 1272, 1582, 1856
LweI GCATC 4 cut(s) 131, 164, 754, 1169
MaeI CTAG 9 cut(s) 54, 291, 543, 918, 1266, 1302, 1562, 1946, 1958
MaeII ACGT 1 cut(s) 1345
MaeIII GTNAC 6 cut(s) 43, 439, 620, 709, 1078, 1585
MalI GATC 5 cut(s) 417, 588, 1180, 1363, 1577
MboI GATC 5 cut(s) 415, 586, 1178, 1361, 1575
MboII GAAGA 7 cut(s) 986, 1230, 1417, 2002, 2005, 2010, 2013
MhlI GDGCHC 4 cut(s) 179, 325, 912, 1111
MluCI AATT 8 cut(s) 78, 162, 913, 1112, 1696, 1883, 1920, 2007
MlyI GAGTC 1 cut(s) 208
MmeI TCCRAC 1 cut(s) 1339
MroNI GCCGGC 1 cut(s) 1497
MroXI GAANNNNTTC 2 cut(s) 78, 1095
MseI TTAA 6 cut(s) 257, 300, 465, 1119, 1377, 2092
MslI CAYNNNNRTG 2 cut(s) 354, 1001
MspI CCGG 4 cut(s) 250, 720, 1498, 1613
MspR9I CCNGG 5 cut(s) 250, 384, 568, 1613, 1614
Mva1269I GAATGC 2 cut(s) 1546, 1820
MvaI CCWGG 2 cut(s) 384, 568
MvnI CGCG 1 cut(s) 1706
MwoI GCNNNNNNNGC 8 cut(s) 388, 601, 1061, 1132, 1154, 1258, 1285, 1826
NaeI GCCGGC 1 cut(s) 1499
NciI CCSGG 3 cut(s) 250, 1613, 1614
NdeI CATATG 1 cut(s) 1638
NdeII GATC 5 cut(s) 415, 586, 1178, 1361, 1575
NgoMIV GCCGGC 1 cut(s) 1497
NheI GCTAGC 1 cut(s) 53
NlaIV GGNNCC 2 cut(s) 1463, 2028
NmuCI GTSAC 4 cut(s) 43, 439, 620, 1078
NspI RCATGY 1 cut(s) 339
PaeR7I CTCGAG 1 cut(s) 83
PctI GAATGC 2 cut(s) 1546, 1820
PdiI GCCGGC 1 cut(s) 1499
PdmI GAANNNNTTC 2 cut(s) 78, 1095
PflFI GACNNNGTC 1 cut(s) 41
PflMI CCANNNNNTGG 1 cut(s) 1149
PkrI GCNGC 8 cut(s) 193, 606, 643, 1159, 1251, 1287, 1597, 1846
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
PpuMI RGGWCCY 1 cut(s) 2026
PshAI GACNNNNGTC 2 cut(s) 218, 785
Psp124BI GAGCTC 1 cut(s) 912
Psp5II RGGWCCY 1 cut(s) 2026
Psp6I CCWGG 2 cut(s) 382, 566
PspFI CCCAGC 1 cut(s) 391
PspGI CCWGG 2 cut(s) 382, 566
PspN4I GGNNCC 2 cut(s) 1463, 2028
PspPI GGNCC 1 cut(s) 2026
PspPPI RGGWCCY 1 cut(s) 2026
PstI CTGCAG 1 cut(s) 1137
PstNI CAGNNNCTG 1 cut(s) 857
PsyI GACNNNGTC 1 cut(s) 41
RsaI GTAC 3 cut(s) 884, 1480, 1803
RsaNI GTAC 3 cut(s) 883, 1479, 1802
RseI CAYNNNNRTG 2 cut(s) 354, 1001
SacI GAGCTC 1 cut(s) 912
SaqAI TTAA 6 cut(s) 257, 300, 465, 1119, 1377, 2092
SatI GCNGC 8 cut(s) 192, 605, 642, 1158, 1250, 1286, 1596, 1845
Sau3AI GATC 5 cut(s) 415, 586, 1178, 1361, 1575
Sau96I GGNCC 1 cut(s) 2026
ScaI AGTACT 1 cut(s) 1480
SchI GAGTC 1 cut(s) 208
ScrFI CCNGG 5 cut(s) 250, 384, 568, 1613, 1614
SduI GDGCHC 4 cut(s) 179, 325, 912, 1111
SfaNI GCATC 4 cut(s) 131, 164, 754, 1169
SfcI CTRYAG 3 cut(s) 1133, 1492, 1535
Sfr274I CTCGAG 1 cut(s) 83
SinI GGWCC 1 cut(s) 2026
SlaI CTCGAG 1 cut(s) 83
SmaI CCCGGG 1 cut(s) 1614
SmiMI CAYNNNNRTG 2 cut(s) 354, 1001
SmlI CTYRAG 2 cut(s) 83, 578
SmoI CTYRAG 2 cut(s) 83, 578
Sse9I AATT 8 cut(s) 78, 162, 913, 1112, 1696, 1883, 1920, 2007
SsiI CCGC 1 cut(s) 1259
SspMI CTAG 9 cut(s) 54, 291, 543, 918, 1266, 1302, 1562, 1946, 1958
SstI GAGCTC 1 cut(s) 912
StyD4I CCNGG 5 cut(s) 248, 382, 566, 1611, 1612
TaaI ACNGT 8 cut(s) 305, 730, 815, 824, 1035, 1585, 1806, 1933
TaiI ACGT 1 cut(s) 1348
TaqI TCGA 6 cut(s) 27, 84, 235, 817, 1724, 1982
TasI AATT 8 cut(s) 78, 162, 913, 1112, 1696, 1883, 1920, 2007
TatI WGTACW 1 cut(s) 1478
Tru1I TTAA 6 cut(s) 257, 300, 465, 1119, 1377, 2092
Tru9I TTAA 6 cut(s) 257, 300, 465, 1119, 1377, 2092
TscAI CASTG 5 cut(s) 325, 622, 735, 1072, 1083
TseFI GTSAC 4 cut(s) 43, 439, 620, 1078
TseI GCWGC 8 cut(s) 191, 604, 641, 1157, 1249, 1285, 1595, 1844
Tsp45I GTSAC 4 cut(s) 43, 439, 620, 1078
TspMI CCCGGG 1 cut(s) 1612
TspRI CASTG 5 cut(s) 325, 622, 735, 1072, 1083
Tth111I GACNNNGTC 1 cut(s) 41
Van91I CCANNNNNTGG 1 cut(s) 1149
VneI GTGCAC 1 cut(s) 321
VpaK11BI GGWCC 1 cut(s) 2026
XapI RAATTY 2 cut(s) 78, 1920
XbaI TCTAGA 1 cut(s) 917
XceI RCATGY 1 cut(s) 339
XhoI CTCGAG 1 cut(s) 83
XmaI CCCGGG 1 cut(s) 1612
XmiI GTMKAC 2 cut(s) 427, 774
XmnI GAANNNNTTC 2 cut(s) 78, 1095
XspI CTAG 9 cut(s) 54, 291, 543, 918, 1266, 1302, 1562, 1946, 1958
ZrmI AGTACT 1 cut(s) 1480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.