ankyrin repeat-containing protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 90, 291 |
| AclWI | GGATC | 1 cut(s) | 694 |
| AcuI | CTGAAG | 2 cut(s) | 195, 632 |
| AfaI | GTAC | 2 cut(s) | 200, 805 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 937 |
| AgsI | TTSAA | 5 cut(s) | 308, 347, 698, 1003, 1013 |
| AhlI | ACTAGT | 1 cut(s) | 886 |
| AjiI | CACGTC | 1 cut(s) | 671 |
| AluBI | AGCT | 3 cut(s) | 574, 610, 646 |
| AluI | AGCT | 3 cut(s) | 574, 610, 646 |
| Alw21I | GWGCWC | 1 cut(s) | 427 |
| Alw26I | GTCTC | 2 cut(s) | 129, 757 |
| AlwI | GGATC | 1 cut(s) | 694 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 173 |
| Ama87I | CYCGRG | 1 cut(s) | 933 |
| ApeKI | GCWGC | 4 cut(s) | 181, 482, 574, 676 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 411 |
| AspLEI | GCGC | 2 cut(s) | 75, 181 |
| AspS9I | GGNCC | 1 cut(s) | 223 |
| AsuHPI | GGTGA | 7 cut(s) | 354, 525, 537, 558, 573, 609, 952 |
| AvaI | CYCGRG | 1 cut(s) | 933 |
| AvaII | GGWCC | 1 cut(s) | 223 |
| BbsI | GAAGAC | 1 cut(s) | 555 |
| Bbv12I | GWGCWC | 1 cut(s) | 427 |
| BbvCI | CCTCAGC | 1 cut(s) | 642 |
| BbvI | GCAGC | 4 cut(s) | 193, 469, 561, 688 |
| BccI | CCATC | 8 cut(s) | 19, 161, 467, 505, 550, 586, 622, 631 |
| BcoDI | GTCTC | 2 cut(s) | 129, 757 |
| BcuI | ACTAGT | 1 cut(s) | 886 |
| BfaI | CTAG | 4 cut(s) | 234, 591, 627, 887 |
| BfmI | CTRYAG | 3 cut(s) | 84, 449, 860 |
| BfoI | RGCGCY | 1 cut(s) | 76 |
| BisI | GCNGC | 4 cut(s) | 182, 483, 575, 677 |
| BlsI | GCNGC | 4 cut(s) | 183, 484, 576, 678 |
| BmcAI | AGTACT | 1 cut(s) | 805 |
| Bme18I | GGWCC | 1 cut(s) | 223 |
| BmeT110I | CYCGRG | 1 cut(s) | 933 |
| BmgBI | CACGTC | 1 cut(s) | 671 |
| BmgT120I | GGNCC | 1 cut(s) | 223 |
| BmiI | GGNNCC | 1 cut(s) | 788 |
| BmsI | GCATC | 2 cut(s) | 70, 494 |
| BoxI | GACNNNNGTC | 1 cut(s) | 101 |
| BpiI | GAAGAC | 1 cut(s) | 555 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 1100 |
| Bpu10I | CCTNAGC | 3 cut(s) | 570, 606, 642 |
| BsaI | GGTCTC | 1 cut(s) | 129 |
| BsaJI | CCNNGG | 1 cut(s) | 1092 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 30, 60 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 937 |
| Bse118I | RCCGGY | 1 cut(s) | 822 |
| Bse1I | ACTGG | 2 cut(s) | 392, 717 |
| BseDI | CCNNGG | 1 cut(s) | 1092 |
| BseGI | GGATG | 2 cut(s) | 153, 485 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 937 |
| BseMII | CTCAG | 2 cut(s) | 656, 798 |
| BseNI | ACTGG | 2 cut(s) | 392, 717 |
| BseXI | GCAGC | 4 cut(s) | 193, 469, 561, 688 |
| BsiHKAI | GWGCWC | 1 cut(s) | 427 |
| BsiHKCI | CYCGRG | 1 cut(s) | 933 |
| BsiSI | CCGG | 2 cut(s) | 36, 823 |
| BslFI | GGGAC | 3 cut(s) | 505, 723, 914 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 937 |
| BsmAI | GTCTC | 2 cut(s) | 129, 757 |
| BsmFI | GGGAC | 3 cut(s) | 505, 723, 914 |
| BsmI | GAATGC | 1 cut(s) | 871 |
| Bso31I | GGTCTC | 1 cut(s) | 129 |
| BsoBI | CYCGRG | 1 cut(s) | 933 |
| Bsp1286I | GDGCHC | 1 cut(s) | 427 |
| Bsp143I | GATC | 3 cut(s) | 21, 503, 686 |
| Bsp19I | CCATGG | 1 cut(s) | 1092 |
| BspCNI | CTCAG | 2 cut(s) | 655, 799 |
| BspLI | GGNNCC | 1 cut(s) | 788 |
| BspMAI | CTGCAG | 1 cut(s) | 453 |
| BspPI | GGATC | 1 cut(s) | 694 |
| BspTNI | GGTCTC | 1 cut(s) | 129 |
| BsrFI | RCCGGY | 1 cut(s) | 822 |
| BsrI | ACTGG | 2 cut(s) | 392, 717 |
| BssAI | RCCGGY | 1 cut(s) | 822 |
| BssECI | CCNNGG | 1 cut(s) | 1092 |
| BssMI | GATC | 3 cut(s) | 21, 503, 686 |
| BssNAI | GTATAC | 1 cut(s) | 91 |
| BssT1I | CCWWGG | 1 cut(s) | 1092 |
| Bst1107I | GTATAC | 1 cut(s) | 91 |
| Bst4CI | ACNGT | 5 cut(s) | 46, 140, 351, 537, 910 |
| BstC8I | GCNNGC | 3 cut(s) | 824, 921, 985 |
| BstDEI | CTNAG | 4 cut(s) | 570, 606, 642, 807 |
| BstDSI | CCRYGG | 1 cut(s) | 1092 |
| BstF5I | GGATG | 2 cut(s) | 153, 485 |
| BstH2I | RGCGCY | 1 cut(s) | 76 |
| BstHHI | GCGC | 2 cut(s) | 75, 181 |
| BstKTI | GATC | 3 cut(s) | 24, 506, 689 |
| BstMAI | GTCTC | 2 cut(s) | 129, 757 |
| BstMBI | GATC | 3 cut(s) | 21, 503, 686 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 377 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 101 |
| BstSFI | CTRYAG | 3 cut(s) | 84, 449, 860 |
| BstV1I | GCAGC | 4 cut(s) | 193, 469, 561, 688 |
| BstV2I | GAAGAC | 1 cut(s) | 555 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 783 |
| BstZ17I | GTATAC | 1 cut(s) | 91 |
| BtgI | CCRYGG | 1 cut(s) | 1092 |
| BtgZI | GCGATG | 1 cut(s) | 585 |
| BtrI | CACGTC | 1 cut(s) | 671 |
| BtsCI | GGATG | 2 cut(s) | 153, 485 |
| BtsI | GCAGTG | 1 cut(s) | 381 |
| BtsIMutI | CAGTG | 2 cut(s) | 381, 399 |
| Cac8I | GCNNGC | 3 cut(s) | 824, 921, 985 |
| CaiI | CAGNNNCTG | 1 cut(s) | 173 |
| CfoI | GCGC | 2 cut(s) | 75, 181 |
| Cfr10I | RCCGGY | 1 cut(s) | 822 |
| Cfr13I | GGNCC | 1 cut(s) | 223 |
| CseI | GACGC | 1 cut(s) | 290 |
| Csp6I | GTAC | 2 cut(s) | 199, 804 |
| CviAII | CATG | 3 cut(s) | 313, 836, 1093 |
| CviQI | GTAC | 2 cut(s) | 199, 804 |
| DdeI | CTNAG | 4 cut(s) | 570, 606, 642, 807 |
| DpnI | GATC | 3 cut(s) | 23, 505, 688 |
| DpnII | GATC | 3 cut(s) | 21, 503, 686 |
| DraI | TTTAAA | 1 cut(s) | 436 |
| Eco130I | CCWWGG | 1 cut(s) | 1092 |
| Eco31I | GGTCTC | 1 cut(s) | 129 |
| Eco47I | GGWCC | 1 cut(s) | 223 |
| Eco57I | CTGAAG | 2 cut(s) | 195, 632 |
| Eco88I | CYCGRG | 1 cut(s) | 933 |
| EcoT14I | CCWWGG | 1 cut(s) | 1092 |
| ErhI | CCWWGG | 1 cut(s) | 1092 |
| FaeI | CATG | 3 cut(s) | 316, 839, 1096 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 729, 761 |
| FaqI | GGGAC | 3 cut(s) | 505, 723, 914 |
| FatI | CATG | 3 cut(s) | 312, 835, 1092 |
| FblI | GTMKAC | 2 cut(s) | 90, 291 |
| Fnu4HI | GCNGC | 4 cut(s) | 182, 483, 575, 677 |
| FokI | GGATG | 2 cut(s) | 140, 472 |
| Fsp4HI | GCNGC | 4 cut(s) | 182, 483, 575, 677 |
| FspBI | CTAG | 4 cut(s) | 234, 591, 627, 887 |
| GlaI | GCGC | 2 cut(s) | 74, 180 |
| GluI | GCNGC | 4 cut(s) | 182, 483, 575, 677 |
| HaeII | RGCGCY | 1 cut(s) | 76 |
| HapII | CCGG | 2 cut(s) | 36, 823 |
| HgaI | GACGC | 1 cut(s) | 290 |
| HhaI | GCGC | 2 cut(s) | 75, 181 |
| Hin1II | CATG | 3 cut(s) | 316, 839, 1096 |
| Hin6I | GCGC | 2 cut(s) | 73, 179 |
| HinP1I | GCGC | 2 cut(s) | 73, 179 |
| HpaII | CCGG | 2 cut(s) | 36, 823 |
| HphI | GGTGA | 7 cut(s) | 354, 525, 537, 558, 573, 609, 952 |
| Hpy166II | GTNNAC | 3 cut(s) | 91, 223, 292 |
| Hpy188I | TCNGA | 4 cut(s) | 21, 175, 417, 1086 |
| Hpy188III | TCNNGA | 7 cut(s) | 134, 234, 305, 404, 501, 949, 1048 |
| Hpy8I | GTNNAC | 3 cut(s) | 91, 223, 292 |
| HpyAV | CCTTC | 3 cut(s) | 239, 653, 822 |
| HpyCH4III | ACNGT | 5 cut(s) | 46, 140, 351, 537, 910 |
| HpyCH4IV | ACGT | 1 cut(s) | 670 |
| HpyCH4V | TGCA | 6 cut(s) | 167, 451, 485, 751, 869, 905 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 377 |
| HpyF3I | CTNAG | 4 cut(s) | 570, 606, 642, 807 |
| HpySE526I | ACGT | 1 cut(s) | 670 |
| Hsp92II | CATG | 3 cut(s) | 316, 839, 1096 |
| HspAI | GCGC | 2 cut(s) | 73, 179 |
| KroI | GCCGGC | 1 cut(s) | 822 |
| KroNI | GCCGGC | 1 cut(s) | 824 |
| Kzo9I | GATC | 3 cut(s) | 21, 503, 686 |
| Lsp1109I | GCAGC | 4 cut(s) | 193, 469, 561, 688 |
| LweI | GCATC | 2 cut(s) | 70, 494 |
| MaeI | CTAG | 4 cut(s) | 234, 591, 627, 887 |
| MaeII | ACGT | 1 cut(s) | 670 |
| MaeIII | GTNAC | 4 cut(s) | 25, 40, 394, 910 |
| MalI | GATC | 3 cut(s) | 23, 505, 688 |
| MboI | GATC | 3 cut(s) | 21, 503, 686 |
| MboII | GAAGA | 2 cut(s) | 555, 742 |
| MhlI | GDGCHC | 1 cut(s) | 427 |
| MluCI | AATT | 3 cut(s) | 428, 1021, 1053 |
| MmeI | TCCRAC | 1 cut(s) | 664 |
| MnlI | CCTC | 7 cut(s) | 236, 278, 323, 651, 700, 847, 1102 |
| MroNI | GCCGGC | 1 cut(s) | 822 |
| MroXI | GAANNNNTTC | 1 cut(s) | 411 |
| MseI | TTAA | 3 cut(s) | 261, 435, 702 |
| MslI | CAYNNNNRTG | 2 cut(s) | 317, 962 |
| MspI | CCGG | 2 cut(s) | 36, 823 |
| Mva1269I | GAATGC | 1 cut(s) | 871 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 377 |
| NaeI | GCCGGC | 1 cut(s) | 824 |
| NcoI | CCATGG | 1 cut(s) | 1092 |
| NdeII | GATC | 3 cut(s) | 21, 503, 686 |
| NgoMIV | GCCGGC | 1 cut(s) | 822 |
| NlaIII | CATG | 3 cut(s) | 316, 839, 1096 |
| NlaIV | GGNNCC | 1 cut(s) | 788 |
| NmuCI | GTSAC | 2 cut(s) | 40, 394 |
| PctI | GAATGC | 1 cut(s) | 871 |
| PdiI | GCCGGC | 1 cut(s) | 824 |
| PdmI | GAANNNNTTC | 1 cut(s) | 411 |
| PkrI | GCNGC | 4 cut(s) | 183, 484, 576, 678 |
| PshAI | GACNNNNGTC | 1 cut(s) | 101 |
| PspN4I | GGNNCC | 1 cut(s) | 788 |
| PspPI | GGNCC | 1 cut(s) | 223 |
| PstI | CTGCAG | 1 cut(s) | 453 |
| PstNI | CAGNNNCTG | 1 cut(s) | 173 |
| RsaI | GTAC | 2 cut(s) | 200, 805 |
| RsaNI | GTAC | 2 cut(s) | 199, 804 |
| RseI | CAYNNNNRTG | 2 cut(s) | 317, 962 |
| SaqAI | TTAA | 3 cut(s) | 261, 435, 702 |
| SatI | GCNGC | 4 cut(s) | 182, 483, 575, 677 |
| Sau3AI | GATC | 3 cut(s) | 21, 503, 686 |
| Sau96I | GGNCC | 1 cut(s) | 223 |
| ScaI | AGTACT | 1 cut(s) | 805 |
| SduI | GDGCHC | 1 cut(s) | 427 |
| SfaNI | GCATC | 2 cut(s) | 70, 494 |
| SfcI | CTRYAG | 3 cut(s) | 84, 449, 860 |
| SinI | GGWCC | 1 cut(s) | 223 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 317, 962 |
| SpeI | ACTAGT | 1 cut(s) | 886 |
| Sse9I | AATT | 3 cut(s) | 428, 1021, 1053 |
| SspMI | CTAG | 4 cut(s) | 234, 591, 627, 887 |
| StyI | CCWWGG | 1 cut(s) | 1092 |
| TaaI | ACNGT | 5 cut(s) | 46, 140, 351, 537, 910 |
| TaiI | ACGT | 1 cut(s) | 673 |
| TaqI | TCGA | 2 cut(s) | 133, 1049 |
| TasI | AATT | 3 cut(s) | 428, 1021, 1053 |
| TatI | WGTACW | 1 cut(s) | 803 |
| Tru1I | TTAA | 3 cut(s) | 261, 435, 702 |
| Tru9I | TTAA | 3 cut(s) | 261, 435, 702 |
| TscAI | CASTG | 2 cut(s) | 388, 399 |
| TseFI | GTSAC | 2 cut(s) | 40, 394 |
| TseI | GCWGC | 4 cut(s) | 181, 482, 574, 676 |
| Tsp45I | GTSAC | 2 cut(s) | 40, 394 |
| TspDTI | ATGAA | 8 cut(s) | 258, 335, 459, 483, 654, 735, 852, 980 |
| TspRI | CASTG | 2 cut(s) | 388, 399 |
| VpaK11BI | GGWCC | 1 cut(s) | 223 |
| XbaI | TCTAGA | 1 cut(s) | 233 |
| XmiI | GTMKAC | 2 cut(s) | 90, 291 |
| XmnI | GAANNNNTTC | 1 cut(s) | 411 |
| XspI | CTAG | 4 cut(s) | 234, 591, 627, 887 |
| ZrmI | AGTACT | 1 cut(s) | 805 |