Rroxscaffold_2G00105170

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000496
Physical Location & Seq
Reverse (-)
28597865 .. 28600396
2532 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00105170.1

Sequence Viewer

Length: 1500 bp
ATGGATCCAAGGCTTTTAGTGTGCATTAGCAGCAACAACATACCTGAATTTATCGACTTACATCATGAAAATGAAGGAATTATTGCGCAAAGAACAGCTGATTCCTTCAACACAGTTTTACACCTTGCTTCGAAACTGGGACAAAATGACATGGTCCTGGAGATTATTAAGTTATGCCCTGAATTGGTTGCTGCTGAAAACAAGTACCTTGAGACTCCAGTTCATGAAGCTTGTCGCCTTGGAAATGCCAACATCTTGAAGCTGCTGTTAGAAGCCAATCCTCAAGCAGCAAAGGATGTTGGGCTTGATCAAACATGTATTAATGTTGCTGCATCCAATGGACACACAGATATTGTCAGAGAGATACTAAACATCTGCCCTAACTATGCTCAAAAGGCTGATGACAATGGAAATTCACCTTTGCACTATGCTTGCAACAAAGGGCACAGGGAAATAACCTGGTTACTGCTACAGCGTGATGTCAATCTGGCCTTGCAGTATAACAACAATGGTTATACACCTTCTCATTTAGCAGCAATAAATGGAAACATATCAATCCTTGAAGACTTCGCTTTGAAAGCTCCAGCGGCTTTTCATTATCTCACAAAAGAAGAGGAGACAATATTTCATCTGACTATTGCTGAGTACTTGATCAACAAAGCAAAGATGGAGATTAATTCACGGAACAGCAAAGGACTTACTGCATTTGACCTCCTCAGTCAGGCCAAAGACAGTGTAGAGGCTCGTCATCTTCAGACAATATTCCATAAAGCCGGTGGTGAAAGATGCACTAATGTGACAAGTACTTGTTCACCAGAAACATCATCTTCAAGGCAGAGTTACCTATTCGAATCAGACATGTCTATTACAAATGAAAATGCTTTACCCTCTTTGCAAGAAATAACCTGCACTCAGCCAGACAACCAATACAAGTCAAGACAGTCATCGGTACTCCACAATCAAGTTCAGGGGAGATTTGACAAGGCAGCATACAAACTAAAGTCCACTCCCTCAACCAACTCGCATTATCAACGCAGCAAGTCTTCGAGCAAGAGGCACTGCAAAGATCATAAGCAGCATAAGATGTACAGAGAGGCGATACAGAATGCGAGGAACACCATAACACTAGTTGCCATTTTAATTGCAACAGTTACTTTTGCTGCTGGAATTACCCCTCCAGGCGGTGTCTATCAGGATGGAGCAATGAAAAGGGAAGTCGATAGCAGGCACAACAACATCTTTCAAGGTTTATGTTGCAGCCACATGGGTGATCATGCCGAGAAATCATGGGACGGCTTTTGTGTTTGTGGCTCTACTGTCTGTCAGTGGTGGCACTCTAGGAACAATTTTCATTGGTTTGGGAGTGATGCTTGTGGATCATTGGCTAAGAAAGTTGAATTGGAGGAAACTGAAAAGGGAATCTGGGGAGGTGCAGATGAAATTGAAAGCCAAAATTCGGATGTTGAGAGCTCATTCCATCAAGGCTATCACTCATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

499

Amino Acids

55.65

Weight (kDa)

6.17

Isoelectric Point (pI)

52.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 8 - 93 1e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 108 - 188 1.7e-14 Ankyrin repeats (3 copies)
Ank PF00023 136 - 162 9.3e-06 Ankyrin repeat
PGG PF13962 365 - 401 2.4e-12 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10340 AT1G10340 AT1G34046 AT1G34050 AT2G24600 AT2G24600 AT2G24600 AT2G24600 AT3G52830 AT5G54700 AT5G54700
fragaria_vesca FvH4_6g32091 FvH4_6g32100 FvH4_6g32100 FvH4_6g32121
malus_domestica MD17G1186800.v1.1 MD17G1187000.v1.1
prunus_persica Prupe.3G061900_v2.0.a1 Prupe.3G062300_v2.0.a1 Prupe.3G062300_v2.0.a1 Prupe.3G062300_v2.0.a1 Prupe.3G062400_v2.0.a1 Prupe.3G065300_v2.0.a1
pyrus_communis pycom17g19590
rosa_chinensis RchiOBHm_Chr1g0353591 RchiOBHm_Chr2g0139761 RchiOBHm_Chr2g0139771 RchiOBHm_Chr2g0139841 RchiOBHm_Chr2g0139851 RchiOBHm_Chr2g0139911
rosa_laevigata RLG00000019831 RLG00000019832 RLG00000019834 RLG00000019835 RLG00000019838 RLG00000028268 RLG00000029493 RLG00000029500 RLG00000029519 RLG00000029774
rosa_multiflora Rmu_co8197386.1_g000001 Rmu_co8359109.1_g000001 Rmu_sc0000545.1_g000005 Rmu_sc0005914.1_g000001 Rmu_sc0030896.1_g000002 Rmu_sc0030896.1_g000003 Rmu_sc0030896.1_g000004 Rmu_sc0032265.1_g000001
rosa_roxburghii Rroxscaffold_2G00105170 Rroxscaffold_2G00105210 Rroxscaffold_2G00105220 Rroxscaffold_4G00301800
rosa_rugosa Rorug01G0236400 Rorug02G0359900 Rorug02G0360000 Rorug02G0360200
rosa_samantha Rh1AG248700 Rh1BG105500 Rh1BG219100 Rh1CG231800 Rh1DG245500 Rh2AG409600 Rh2AG409700 Rh2AG410000 Rh2AG410100 Rh2AG410400 Rh2AG410500 Rh2BG420100 Rh2BG420200 Rh2BG420600 Rh2BG420700 Rh2CG395900 Rh2CG396000 Rh2CG396200 Rh2CG396500 Rh2CG396600 Rh2DG429400 Rh2DG429500 Rh2DG429800 Rh2DG430100 Rh2DG430200
rosa_wichuraiana Rw1G021690 Rw2G033350 Rw2G033400 Rw2G033410 Rw2G033570 Rw2G033620 Rw2G033630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 87
Acc36I ACCTGC 1 cut(s) 914
AciI CCGC 2 cut(s) 587, 1182
AclWI GGATC 2 cut(s) 12, 1384
AcsI RAATTY 3 cut(s) 47, 412, 1453
AcuI CTGAAG 1 cut(s) 737
AfaI GTAC 5 cut(s) 206, 647, 805, 951, 1088
AfiI CCNNNNNNNGG 4 cut(s) 184, 721, 1181, 1456
AflIII ACRYGT 2 cut(s) 314, 858
AgsI TTSAA 8 cut(s) 109, 259, 563, 577, 831, 1244, 1397, 1445
AhlI ACTAGT 1 cut(s) 1126
AjnI CCWGG 3 cut(s) 156, 458, 1177
AjuI GAANNNNNNNTTGG 2 cut(s) 1382, 1414
AleI CACNNNNGTG 2 cut(s) 794, 1266
AluBI AGCT 5 cut(s) 98, 230, 262, 581, 1470
AluI AGCT 5 cut(s) 98, 230, 262, 581, 1470
Alw21I GWGCWC 1 cut(s) 1472
Alw26I GTCTC 2 cut(s) 206, 611
AlwI GGATC 2 cut(s) 12, 1384
AoxI GGCC 2 cut(s) 489, 723
ApoI RAATTY 3 cut(s) 47, 412, 1453
AseI ATTAAT 2 cut(s) 321, 675
AspLEI GCGC 1 cut(s) 88
AspS9I GGNCC 1 cut(s) 154
AsuHPI GGTGA 4 cut(s) 408, 791, 804, 1280
AsuII TTCGAA 2 cut(s) 131, 849
AvaII GGWCC 1 cut(s) 154
BaeGI GKGCMC 1 cut(s) 447
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 1472
BbsI GAAGAC 2 cut(s) 570, 1035
Bbv12I GWGCWC 1 cut(s) 1472
BccI CCATC 3 cut(s) 661, 1190, 1485
BceAI ACGGC 1 cut(s) 1309
BciT130I CCWGG 3 cut(s) 158, 460, 1179
BclI TGATCA 3 cut(s) 307, 651, 1270
BcoDI GTCTC 2 cut(s) 206, 611
BcuI ACTAGT 1 cut(s) 1126
BfaI CTAG 2 cut(s) 1127, 1338
BfmI CTRYAG 1 cut(s) 470
BfuAI ACCTGC 1 cut(s) 914
BmcAI AGTACT 2 cut(s) 647, 805
Bme1390I CCNGG 3 cut(s) 158, 460, 1179
Bme18I GGWCC 1 cut(s) 154
BmgT120I GGNCC 1 cut(s) 154
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 3 cut(s) 158, 460, 1179
BmrI ACTGGG 1 cut(s) 146
BmsI GCATC 3 cut(s) 341, 776, 1357
BmuI ACTGGG 1 cut(s) 146
BpiI GAAGAC 2 cut(s) 570, 1035
BpmI CTGGAG 4 cut(s) 179, 201, 567, 1161
Bpu14I TTCGAA 2 cut(s) 131, 849
BpuEI CTTGAG 2 cut(s) 230, 267
BsaBI GATNNNNATC 1 cut(s) 483
BsaJI CCNNGG 2 cut(s) 8, 238
Bsc4I CCNNNNNNNGG 4 cut(s) 184, 721, 1181, 1456
Bse118I RCCGGY 1 cut(s) 773
Bse1I ACTGG 2 cut(s) 141, 218
Bse3DI GCAATG 1 cut(s) 1209
Bse8I GATNNNNATC 1 cut(s) 483
BseBI CCWGG 3 cut(s) 158, 460, 1179
BseDI CCNNGG 2 cut(s) 8, 238
BseGI GGATG 4 cut(s) 301, 332, 1201, 1465
BseJI GATNNNNATC 1 cut(s) 483
BseLI CCNNNNNNNGG 4 cut(s) 184, 721, 1181, 1456
BseMI GCAATG 1 cut(s) 1209
BseMII CTCAG 3 cut(s) 633, 730, 926
BseNI ACTGG 2 cut(s) 141, 218
BseRI GAGGAG 2 cut(s) 629, 704
BseSI GKGCMC 1 cut(s) 447
BsgI GTGCAG 2 cut(s) 892, 1452
BshFI GGCC 2 cut(s) 491, 725
BsiHKAI GWGCWC 1 cut(s) 1472
BsiSI CCGG 1 cut(s) 774
BslFI GGGAC 2 cut(s) 153, 1304
BslI CCNNNNNNNGG 4 cut(s) 184, 721, 1181, 1456
BsmAI GTCTC 2 cut(s) 206, 611
BsmFI GGGAC 2 cut(s) 153, 1304
BsmI GAATGC 1 cut(s) 1111
BsnI GGCC 2 cut(s) 491, 725
Bsp119I TTCGAA 2 cut(s) 131, 849
Bsp1286I GDGCHC 2 cut(s) 447, 1472
Bsp1407I TGTACA 1 cut(s) 1086
Bsp143I GATC 6 cut(s) 4, 307, 651, 1066, 1270, 1376
BspACI CCGC 2 cut(s) 587, 1182
BspANI GGCC 2 cut(s) 491, 725
BspCNI CTCAG 3 cut(s) 634, 729, 925
BspHI TCATGA 2 cut(s) 64, 223
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 914
BspPI GGATC 2 cut(s) 12, 1384
BspT104I TTCGAA 2 cut(s) 131, 849
BsrDI GCAATG 1 cut(s) 1209
BsrFI RCCGGY 1 cut(s) 773
BsrGI TGTACA 1 cut(s) 1086
BsrI ACTGG 2 cut(s) 141, 218
BssAI RCCGGY 1 cut(s) 773
BssECI CCNNGG 2 cut(s) 8, 238
BssMI GATC 6 cut(s) 4, 307, 651, 1066, 1270, 1376
BssT1I CCWWGG 2 cut(s) 8, 238
Bst2UI CCWGG 3 cut(s) 158, 460, 1179
Bst4CI ACNGT 5 cut(s) 115, 734, 942, 1150, 1318
Bst6I CTCTTC 1 cut(s) 606
BstAUI TGTACA 1 cut(s) 1086
BstBI TTCGAA 2 cut(s) 131, 849
BstC8I GCNNGC 2 cut(s) 433, 1226
BstDEI CTNAG 4 cut(s) 642, 716, 912, 1386
BstENI CCTNNNNNAGG 1 cut(s) 719
BstF5I GGATG 4 cut(s) 301, 332, 1201, 1465
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 6 cut(s) 7, 310, 654, 1069, 1273, 1379
BstMAI GTCTC 2 cut(s) 206, 611
BstMBI GATC 6 cut(s) 4, 307, 651, 1066, 1270, 1376
BstMWI GCNNNNNNNGC 4 cut(s) 30, 395, 578, 587
BstNI CCWGG 3 cut(s) 158, 460, 1179
BstNSI RCATGY 2 cut(s) 318, 862
BstSCI CCNGG 3 cut(s) 156, 458, 1177
BstSFI CTRYAG 1 cut(s) 470
BstSLI GKGCMC 1 cut(s) 447
BstV2I GAAGAC 2 cut(s) 570, 1035
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 2 cut(s) 491, 725
BtsCI GGATG 4 cut(s) 301, 332, 1201, 1465
BtsI GCAGTG 1 cut(s) 1057
BtsIMutI CAGTG 3 cut(s) 739, 1057, 1331
BveI ACCTGC 1 cut(s) 914
Cac8I GCNNGC 2 cut(s) 433, 1226
CciI TCATGA 2 cut(s) 64, 223
CfoI GCGC 1 cut(s) 88
Cfr10I RCCGGY 1 cut(s) 773
Cfr13I GGNCC 1 cut(s) 154
CsiI ACCWGGT 1 cut(s) 458
Csp6I GTAC 5 cut(s) 205, 646, 804, 950, 1087
CviAII CATG 8 cut(s) 65, 151, 224, 315, 859, 1264, 1274, 1287
CviQI GTAC 5 cut(s) 205, 646, 804, 950, 1087
DdeI CTNAG 4 cut(s) 642, 716, 912, 1386
DpnI GATC 6 cut(s) 6, 309, 653, 1068, 1272, 1378
DpnII GATC 6 cut(s) 4, 307, 651, 1066, 1270, 1376
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
Ecl136II GAGCTC 1 cut(s) 1470
Eco130I CCWWGG 2 cut(s) 8, 238
Eco24I GRGCYC 1 cut(s) 1472
Eco47I GGWCC 1 cut(s) 154
Eco53kI GAGCTC 1 cut(s) 1470
Eco57I CTGAAG 1 cut(s) 737
EcoICRI GAGCTC 1 cut(s) 1470
EcoNI CCTNNNNNAGG 1 cut(s) 719
EcoRII CCWGG 3 cut(s) 156, 458, 1177
EcoT14I CCWWGG 2 cut(s) 8, 238
EcoT38I GRGCYC 1 cut(s) 1472
ErhI CCWWGG 2 cut(s) 8, 238
FaeI CATG 8 cut(s) 68, 154, 227, 318, 862, 1267, 1277, 1290
FaqI GGGAC 2 cut(s) 153, 1304
FatI CATG 8 cut(s) 64, 150, 223, 314, 858, 1263, 1273, 1286
FbaI TGATCA 3 cut(s) 307, 651, 1270
FokI GGATG 4 cut(s) 308, 319, 1208, 1472
FriOI GRGCYC 1 cut(s) 1472
FspBI CTAG 2 cut(s) 1127, 1338
FspI TGCGCA 1 cut(s) 87
GlaI GCGC 1 cut(s) 87
GsuI CTGGAG 4 cut(s) 179, 201, 567, 1161
HaeIII GGCC 2 cut(s) 491, 725
HapII CCGG 1 cut(s) 774
HhaI GCGC 1 cut(s) 88
Hin1II CATG 8 cut(s) 68, 154, 227, 318, 862, 1267, 1277, 1290
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HindIII AAGCTT 1 cut(s) 228
HinfI GANTC 4 cut(s) 101, 214, 851, 1419
HpaII CCGG 1 cut(s) 774
HphI GGTGA 4 cut(s) 408, 791, 804, 1280
Hpy166II GTNNAC 2 cut(s) 812, 1005
Hpy188I TCNGA 5 cut(s) 359, 633, 756, 856, 1459
Hpy188III TCNNGA 5 cut(s) 65, 224, 256, 936, 1193
Hpy8I GTNNAC 2 cut(s) 812, 1005
HpyAV CCTTC 3 cut(s) 68, 115, 531
HpyCH4III ACNGT 5 cut(s) 115, 734, 942, 1150, 1318
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 395, 578, 587
HpyF3I CTNAG 4 cut(s) 642, 716, 912, 1386
Hsp92II CATG 8 cut(s) 68, 154, 227, 318, 862, 1267, 1277, 1290
HspAI GCGC 1 cut(s) 86
Ksp22I TGATCA 3 cut(s) 307, 651, 1270
Kzo9I GATC 6 cut(s) 4, 307, 651, 1066, 1270, 1376
LmnI GCTCC 2 cut(s) 586, 1199
LweI GCATC 3 cut(s) 341, 776, 1357
MabI ACCWGGT 1 cut(s) 458
MaeI CTAG 2 cut(s) 1127, 1338
MaeIII GTNAC 4 cut(s) 462, 796, 839, 1150
MalI GATC 6 cut(s) 6, 309, 653, 1068, 1272, 1378
MboI GATC 6 cut(s) 4, 307, 651, 1066, 1270, 1376
MboII GAAGA 5 cut(s) 575, 623, 743, 819, 1035
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 447, 1472
MlyI GAGTC 1 cut(s) 208
MseI TTAA 4 cut(s) 168, 321, 675, 1139
MslI CAYNNNNRTG 3 cut(s) 69, 794, 1266
MspA1I CMGCKG 2 cut(s) 98, 587
MspI CCGG 1 cut(s) 774
MspR9I CCNGG 3 cut(s) 158, 460, 1179
Mva1269I GAATGC 1 cut(s) 1111
MvaI CCWGG 3 cut(s) 158, 460, 1179
MwoI GCNNNNNNNGC 4 cut(s) 30, 395, 578, 587
NdeII GATC 6 cut(s) 4, 307, 651, 1066, 1270, 1376
NlaIII CATG 8 cut(s) 68, 154, 227, 318, 862, 1267, 1277, 1290
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 1303
NmuCI GTSAC 1 cut(s) 796
NsbI TGCGCA 1 cut(s) 87
NspI RCATGY 2 cut(s) 318, 862
NspV TTCGAA 2 cut(s) 131, 849
OliI CACNNNNGTG 2 cut(s) 794, 1266
PagI TCATGA 2 cut(s) 64, 223
PciI ACATGT 2 cut(s) 314, 858
PctI GAATGC 1 cut(s) 1111
PfeI GAWTC 3 cut(s) 101, 851, 1419
PflFI GACNNNGTC 1 cut(s) 152
PfoI TCCNGGA 1 cut(s) 156
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
PscI ACATGT 2 cut(s) 314, 858
PshBI ATTAAT 2 cut(s) 321, 675
Psp124BI GAGCTC 1 cut(s) 1472
Psp6I CCWGG 3 cut(s) 156, 458, 1177
PspGI CCWGG 3 cut(s) 156, 458, 1177
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 154
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 98
RsaI GTAC 5 cut(s) 206, 647, 805, 951, 1088
RsaNI GTAC 5 cut(s) 205, 646, 804, 950, 1087
RseI CAYNNNNRTG 3 cut(s) 69, 794, 1266
SacI GAGCTC 1 cut(s) 1472
SaqAI TTAA 4 cut(s) 168, 321, 675, 1139
Sau3AI GATC 6 cut(s) 4, 307, 651, 1066, 1270, 1376
Sau96I GGNCC 1 cut(s) 154
ScaI AGTACT 2 cut(s) 647, 805
SchI GAGTC 1 cut(s) 208
ScrFI CCNGG 3 cut(s) 158, 460, 1179
SduI GDGCHC 2 cut(s) 447, 1472
SexAI ACCWGGT 1 cut(s) 458
SfaNI GCATC 3 cut(s) 341, 776, 1357
SfcI CTRYAG 1 cut(s) 470
SfuI TTCGAA 2 cut(s) 131, 849
SinI GGWCC 1 cut(s) 154
SmiMI CAYNNNNRTG 3 cut(s) 69, 794, 1266
SmlI CTYRAG 2 cut(s) 209, 282
SmoI CTYRAG 2 cut(s) 209, 282
SpeI ACTAGT 1 cut(s) 1126
SsiI CCGC 2 cut(s) 587, 1182
SspI AATATT 2 cut(s) 624, 762
SspMI CTAG 2 cut(s) 1127, 1338
SstI GAGCTC 1 cut(s) 1472
StyD4I CCNGG 3 cut(s) 156, 458, 1177
StyI CCWWGG 2 cut(s) 8, 238
TaaI ACNGT 5 cut(s) 115, 734, 942, 1150, 1318
TaqI TCGA 5 cut(s) 54, 131, 849, 1046, 1218
TatI WGTACW 3 cut(s) 645, 803, 1086
TauI GCSGC 1 cut(s) 590
TfiI GAWTC 3 cut(s) 101, 851, 1419
Tru1I TTAA 4 cut(s) 168, 321, 675, 1139
Tru9I TTAA 4 cut(s) 168, 321, 675, 1139
TscAI CASTG 3 cut(s) 739, 1064, 1331
TseFI GTSAC 1 cut(s) 796
Tsp45I GTSAC 1 cut(s) 796
TspGWI ACGGA 1 cut(s) 697
TspRI CASTG 3 cut(s) 739, 1064, 1331
Tth111I GACNNNGTC 1 cut(s) 152
VpaK11BI GGWCC 1 cut(s) 154
VspI ATTAAT 2 cut(s) 321, 675
XagI CCTNNNNNAGG 1 cut(s) 719
XapI RAATTY 3 cut(s) 47, 412, 1453
XceI RCATGY 2 cut(s) 318, 862
XcmI CCANNNNNNNNNTGG 1 cut(s) 773
XspI CTAG 2 cut(s) 1127, 1338
ZrmI AGTACT 2 cut(s) 647, 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.