pycom02g18870

TdcA1-ORF2 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001541
Physical Location & Seq
Reverse (-)
16927775 .. 16928799
1025 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g18870.2

Sequence Viewer

Length: 921 bp
ATGGAGCTAGCACATTGGTTCATACTCAACAATTGTGATGAGGTTTTGCCATACCTTGAAGAGCATGAACGGTTGATGAAGCGGAAACATCCTTCACATTTATATGCCAAGAAGCACCGTGAATTGTTTCCTTCGTGGTTTCACGAACATATGAACAAATTGAAGGAATTGAATTCACCCTCTTACGATGAAGAATTATATAACTTGGCAAGAGGACCGCTTCACGTTGAATTGTTCTCAGGTTGTCATGTCAACGGGATCAAGTTCCTGGGGGCAACACGTGATGACAAGTCGTCTACTCAAAATAGTGGTGTCCACGTCGCGGGTGCGGGCGACAGTGAAGACATTGATTTTTATGGTAAACTAACTAGTGTGGTACAATTGCTTTACAAAGACAGGTGTCAAGTGATACTGTTTAAGTGTCTTTGGTTTGATACAAACCCACATAACCGAACGAGTGTTAAGCGAGACCATGGTTTACTATCAATAAACACTACCAGACGTTGGTACGATGAAGACCCATACATTTTGGCAACTATGGTGAAACAAATATTCTTTCTAGACGACCCTAAAGCCGGCAATGGTTGGAAAGTTGTTCAGAAGATTGATCGAAGAGGGCTATATGATATTCCGGAACTAAATCAAGATGACAACGACGACAACATCGCTGACCAACAGTTATCATCTTTGATAAAAATTGGTGAAGAAACACTCCGAGATACTAATGTTGTCCAAGAACCGTTTGAAGTAGCTGGAGTTCCCGAATTCGAAATACCAATAGAGTCAATTTCAATTGACCTTGGTGATCTGCCACGATACAATGCACCGGAAGAGGCAAATGAAGATGAGGATGAATGGAAATCCGAAAATGATAGTAGTGAGGATAGCGAGAGTTATTATTGTAGTTCAGATGAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transpos_assoc Transposase_21 Transposase_24

Protein Analysis

307

Amino Acids

35.45

Weight (kDa)

4.7

Isoelectric Point (pI)

48.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 504
AccI GTMKAC 1 cut(s) 296
AccII CGCG 1 cut(s) 323
AccIII TCCGGA 1 cut(s) 631
AciI CCGC 4 cut(s) 82, 218, 323, 329
AclWI GGATC 1 cut(s) 266
AcsI RAATTY 2 cut(s) 172, 764
AcvI CACGTG 1 cut(s) 281
AfaI GTAC 2 cut(s) 378, 509
AfiI CCNNNNNNNGG 3 cut(s) 322, 504, 575
AflIII ACRYGT 1 cut(s) 278
AgsI TTSAA 6 cut(s) 59, 163, 172, 230, 746, 792
AhdI GACNNNNNGTC 1 cut(s) 292
AhlI ACTAGT 1 cut(s) 368
AjiI CACGTC 1 cut(s) 319
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 2 cut(s) 7, 752
AluI AGCT 2 cut(s) 7, 752
Alw26I GTCTC 1 cut(s) 462
AlwI GGATC 1 cut(s) 266
Aor13HI TCCGGA 1 cut(s) 631
ApoI RAATTY 2 cut(s) 172, 764
Asp700I GAANNNNTTC 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 215
AsuHPI GGTGA 4 cut(s) 168, 553, 713, 815
AsuII TTCGAA 1 cut(s) 768
AsuNHI GCTAGC 1 cut(s) 7
AvaII GGWCC 1 cut(s) 215
BaeI ACNNNNGTAYC 2 cut(s) 711, 744
BbrPI CACGTG 1 cut(s) 281
BbsI GAAGAC 2 cut(s) 348, 522
BciT130I CCWGG 1 cut(s) 269
BcoDI GTCTC 1 cut(s) 462
BcuI ACTAGT 1 cut(s) 368
BfaI CTAG 3 cut(s) 8, 369, 560
Bme1390I CCNGG 1 cut(s) 269
Bme18I GGWCC 1 cut(s) 215
BmeRI GACNNNNNGTC 1 cut(s) 292
BmgBI CACGTC 1 cut(s) 319
BmgT120I GGNCC 1 cut(s) 215
BmrFI CCNGG 1 cut(s) 269
BmtI GCTAGC 1 cut(s) 11
BoxI GACNNNNGTC 1 cut(s) 399
BpiI GAAGAC 2 cut(s) 348, 522
BpmI CTGGAG 1 cut(s) 774
Bpu14I TTCGAA 1 cut(s) 768
BsaAI YACGTR 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 462
BsaJI CCNNGG 3 cut(s) 268, 472, 799
BsaWI WCCGGW 2 cut(s) 631, 826
Bsc4I CCNNNNNNNGG 3 cut(s) 322, 504, 575
Bse118I RCCGGY 1 cut(s) 575
Bse3DI GCAATG 1 cut(s) 586
BseAI TCCGGA 1 cut(s) 631
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 3 cut(s) 268, 472, 799
BseGI GGATG 2 cut(s) 88, 856
BseLI CCNNNNNNNGG 3 cut(s) 322, 504, 575
BseMI GCAATG 1 cut(s) 586
BseMII CTCAG 1 cut(s) 252
Bsh1236I CGCG 1 cut(s) 323
BsiSI CCGG 3 cut(s) 576, 632, 827
BslI CCNNNNNNNGG 3 cut(s) 322, 504, 575
BsmAI GTCTC 1 cut(s) 462
Bso31I GGTCTC 1 cut(s) 462
Bsp119I TTCGAA 1 cut(s) 768
Bsp13I TCCGGA 1 cut(s) 631
Bsp143I GATC 3 cut(s) 258, 607, 805
Bsp19I CCATGG 1 cut(s) 472
BspACI CCGC 4 cut(s) 82, 218, 323, 329
BspCNI CTCAG 1 cut(s) 251
BspEI TCCGGA 1 cut(s) 631
BspFNI CGCG 1 cut(s) 323
BspOI GCTAGC 1 cut(s) 11
BspPI GGATC 1 cut(s) 266
BspQI GCTCTTC 1 cut(s) 54
BspT104I TTCGAA 1 cut(s) 768
BspTNI GGTCTC 1 cut(s) 462
BsrDI GCAATG 1 cut(s) 586
BsrFI RCCGGY 1 cut(s) 575
BssAI RCCGGY 1 cut(s) 575
BssECI CCNNGG 3 cut(s) 268, 472, 799
BssMI GATC 3 cut(s) 258, 607, 805
BssT1I CCWWGG 2 cut(s) 472, 799
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 6 cut(s) 72, 119, 338, 414, 678, 741
Bst6I CTCTTC 3 cut(s) 54, 607, 825
BstBAI YACGTR 1 cut(s) 281
BstBI TTCGAA 1 cut(s) 768
BstC8I GCNNGC 3 cut(s) 9, 331, 577
BstDEI CTNAG 1 cut(s) 238
BstDSI CCRYGG 1 cut(s) 472
BstF5I GGATG 2 cut(s) 88, 856
BstFNI CGCG 1 cut(s) 323
BstKTI GATC 3 cut(s) 261, 610, 808
BstMAI GTCTC 1 cut(s) 462
BstMBI GATC 3 cut(s) 258, 607, 805
BstNI CCWGG 1 cut(s) 269
BstPAI GACNNNNGTC 1 cut(s) 399
BstSCI CCNGG 1 cut(s) 267
BstUI CGCG 1 cut(s) 323
BstV2I GAAGAC 2 cut(s) 348, 522
BtgI CCRYGG 1 cut(s) 472
BtgZI GCGATG 1 cut(s) 649
BtrI CACGTC 1 cut(s) 319
BtsCI GGATG 2 cut(s) 88, 856
BtsIMutI CAGTG 1 cut(s) 343
Cac8I GCNNGC 3 cut(s) 9, 331, 577
Cfr10I RCCGGY 1 cut(s) 575
Cfr13I GGNCC 1 cut(s) 215
Csp6I GTAC 2 cut(s) 377, 508
CviAII CATG 3 cut(s) 65, 248, 473
CviJI RGCY 4 cut(s) 7, 575, 619, 752
CviKI_1 RGCY 4 cut(s) 7, 575, 619, 752
CviQI GTAC 2 cut(s) 377, 508
DdeI CTNAG 1 cut(s) 238
DpnI GATC 3 cut(s) 260, 609, 807
DpnII GATC 3 cut(s) 258, 607, 805
DriI GACNNNNNGTC 1 cut(s) 292
Eam1104I CTCTTC 3 cut(s) 54, 607, 825
Eam1105I GACNNNNNGTC 1 cut(s) 292
EarI CTCTTC 3 cut(s) 54, 607, 825
Eco130I CCWWGG 2 cut(s) 472, 799
Eco31I GGTCTC 1 cut(s) 462
Eco47I GGWCC 1 cut(s) 215
Eco72I CACGTG 1 cut(s) 281
EcoRI GAATTC 2 cut(s) 172, 764
EcoRII CCWGG 1 cut(s) 267
EcoT14I CCWWGG 2 cut(s) 472, 799
ErhI CCWWGG 2 cut(s) 472, 799
FaeI CATG 3 cut(s) 68, 251, 476
FatI CATG 3 cut(s) 64, 247, 472
FauI CCCGC 2 cut(s) 316, 322
FauNDI CATATG 1 cut(s) 150
FblI GTMKAC 1 cut(s) 296
FokI GGATG 2 cut(s) 75, 863
FspBI CTAG 3 cut(s) 8, 369, 560
GsuI CTGGAG 1 cut(s) 774
HapII CCGG 3 cut(s) 576, 632, 827
Hin1II CATG 3 cut(s) 68, 251, 476
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 1 cut(s) 782
HpaII CCGG 3 cut(s) 576, 632, 827
HphI GGTGA 4 cut(s) 168, 553, 713, 815
Hpy166II GTNNAC 5 cut(s) 253, 297, 316, 362, 479
Hpy188I TCNGA 4 cut(s) 600, 716, 865, 910
Hpy188III TCNNGA 5 cut(s) 143, 560, 632, 644, 761
Hpy8I GTNNAC 5 cut(s) 253, 297, 316, 362, 479
Hpy99I CGWCG 2 cut(s) 323, 659
HpyAV CCTTC 3 cut(s) 102, 141, 157
HpyCH4III ACNGT 6 cut(s) 72, 119, 338, 414, 678, 741
HpyCH4IV ACGT 4 cut(s) 225, 280, 318, 502
HpyCH4V TGCA 1 cut(s) 824
HpyF3I CTNAG 1 cut(s) 238
HpySE526I ACGT 4 cut(s) 225, 280, 318, 502
Hsp92II CATG 3 cut(s) 68, 251, 476
Kpn2I TCCGGA 1 cut(s) 631
KroI GCCGGC 1 cut(s) 575
KroNI GCCGGC 1 cut(s) 577
Kzo9I GATC 3 cut(s) 258, 607, 805
LguI GCTCTTC 1 cut(s) 54
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 9 cut(s) 225, 254, 281, 382, 511, 589, 645, 738, 840
MaeI CTAG 3 cut(s) 8, 369, 560
MaeII ACGT 4 cut(s) 225, 280, 318, 502
MalI GATC 3 cut(s) 260, 609, 807
MboI GATC 3 cut(s) 258, 607, 805
MboII GAAGA 9 cut(s) 71, 203, 353, 527, 613, 624, 716, 842, 854
MfeI CAATTG 3 cut(s) 31, 380, 792
MlyI GAGTC 1 cut(s) 791
MmeI TCCRAC 1 cut(s) 566
MnlI CCTC 8 cut(s) 34, 190, 206, 608, 826, 841, 874, 907
MroI TCCGGA 1 cut(s) 631
MroNI GCCGGC 1 cut(s) 575
MroXI GAANNNNTTC 1 cut(s) 126
MseI TTAA 3 cut(s) 417, 462, 919
MslI CAYNNNNRTG 1 cut(s) 102
MspI CCGG 3 cut(s) 576, 632, 827
MspR9I CCNGG 1 cut(s) 269
MunI CAATTG 3 cut(s) 31, 380, 792
MvaI CCWGG 1 cut(s) 269
MvnI CGCG 1 cut(s) 323
NaeI GCCGGC 1 cut(s) 577
NcoI CCATGG 1 cut(s) 472
NdeI CATATG 1 cut(s) 150
NdeII GATC 3 cut(s) 258, 607, 805
NgoMIV GCCGGC 1 cut(s) 575
NheI GCTAGC 1 cut(s) 7
NlaIII CATG 3 cut(s) 68, 251, 476
NspV TTCGAA 1 cut(s) 768
PciSI GCTCTTC 1 cut(s) 54
PdiI GCCGGC 1 cut(s) 577
PdmI GAANNNNTTC 1 cut(s) 126
PflMI CCANNNNNTGG 1 cut(s) 504
PleI GAGTC 1 cut(s) 790
PmaCI CACGTG 1 cut(s) 281
PmlI CACGTG 1 cut(s) 281
PpsI GAGTC 1 cut(s) 790
Ppu21I YACGTR 1 cut(s) 281
PshAI GACNNNNGTC 1 cut(s) 399
Psp6I CCWGG 1 cut(s) 267
PspCI CACGTG 1 cut(s) 281
PspGI CCWGG 1 cut(s) 267
PspPI GGNCC 1 cut(s) 215
PsrI GAACNNNNNNTAC 2 cut(s) 741, 773
RsaI GTAC 2 cut(s) 378, 509
RsaNI GTAC 2 cut(s) 377, 508
RseI CAYNNNNRTG 1 cut(s) 102
SapI GCTCTTC 1 cut(s) 54
SaqAI TTAA 3 cut(s) 417, 462, 919
Sau3AI GATC 3 cut(s) 258, 607, 805
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 1 cut(s) 791
ScrFI CCNGG 1 cut(s) 269
SfuI TTCGAA 1 cut(s) 768
SinI GGWCC 1 cut(s) 215
SmiMI CAYNNNNRTG 1 cut(s) 102
SpeI ACTAGT 1 cut(s) 368
SsiI CCGC 4 cut(s) 82, 218, 323, 329
SspI AATATT 1 cut(s) 552
SspMI CTAG 3 cut(s) 8, 369, 560
StyD4I CCNGG 1 cut(s) 267
StyI CCWWGG 2 cut(s) 472, 799
TaaI ACNGT 6 cut(s) 72, 119, 338, 414, 678, 741
TaiI ACGT 4 cut(s) 228, 283, 321, 505
TaqI TCGA 2 cut(s) 610, 768
Tru1I TTAA 3 cut(s) 417, 462, 919
Tru9I TTAA 3 cut(s) 417, 462, 919
TscAI CASTG 1 cut(s) 343
TspDTI ATGAA 8 cut(s) 10, 81, 92, 167, 204, 528, 855, 867
TspRI CASTG 1 cut(s) 343
Van91I CCANNNNNTGG 1 cut(s) 504
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 2 cut(s) 172, 764
XbaI TCTAGA 1 cut(s) 559
XmiI GTMKAC 1 cut(s) 296
XmnI GAANNNNTTC 1 cut(s) 126
XspI CTAG 3 cut(s) 8, 369, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.