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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 217 |
| AclWI | GGATC | 1 cut(s) | 12 |
| AcsI | RAATTY | 2 cut(s) | 263, 292 |
| AcuI | CTGAAG | 1 cut(s) | 141 |
| AgsI | TTSAA | 2 cut(s) | 245, 298 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 70 |
| AjnI | CCWGG | 1 cut(s) | 142 |
| AluBI | AGCT | 3 cut(s) | 95, 125, 181 |
| AluI | AGCT | 3 cut(s) | 95, 125, 181 |
| AlwI | GGATC | 1 cut(s) | 12 |
| ApoI | RAATTY | 2 cut(s) | 263, 292 |
| AspS9I | GGNCC | 1 cut(s) | 72 |
| AvaII | GGWCC | 1 cut(s) | 72 |
| BbvCI | CCTCAGC | 1 cut(s) | 57 |
| BccI | CCATC | 1 cut(s) | 62 |
| BceAI | ACGGC | 1 cut(s) | 183 |
| BciT130I | CCWGG | 1 cut(s) | 144 |
| Bme1390I | CCNGG | 1 cut(s) | 144 |
| Bme18I | GGWCC | 1 cut(s) | 72 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 70 |
| BmgT120I | GGNCC | 1 cut(s) | 72 |
| BmiI | GGNNCC | 2 cut(s) | 73, 148 |
| BmrFI | CCNGG | 1 cut(s) | 144 |
| BmsI | GCATC | 2 cut(s) | 174, 243 |
| Bpu10I | CCTNAGC | 1 cut(s) | 57 |
| BsaJI | CCNNGG | 1 cut(s) | 142 |
| Bse3DI | GCAATG | 1 cut(s) | 187 |
| BseBI | CCWGG | 1 cut(s) | 144 |
| BseDI | CCNNGG | 1 cut(s) | 142 |
| BseGI | GGATG | 2 cut(s) | 28, 258 |
| BseMI | GCAATG | 1 cut(s) | 187 |
| BseMII | CTCAG | 1 cut(s) | 48 |
| Bsp143I | GATC | 1 cut(s) | 4 |
| BspCNI | CTCAG | 1 cut(s) | 49 |
| BspLI | GGNNCC | 2 cut(s) | 73, 148 |
| BspPI | GGATC | 1 cut(s) | 12 |
| BsrDI | GCAATG | 1 cut(s) | 187 |
| BssECI | CCNNGG | 1 cut(s) | 142 |
| BssMI | GATC | 1 cut(s) | 4 |
| Bst2UI | CCWGG | 1 cut(s) | 144 |
| BstC8I | GCNNGC | 1 cut(s) | 117 |
| BstDEI | CTNAG | 1 cut(s) | 57 |
| BstF5I | GGATG | 2 cut(s) | 28, 258 |
| BstKTI | GATC | 1 cut(s) | 7 |
| BstMBI | GATC | 1 cut(s) | 4 |
| BstNI | CCWGG | 1 cut(s) | 144 |
| BstNSI | RCATGY | 1 cut(s) | 56 |
| BstSCI | CCNGG | 1 cut(s) | 142 |
| BtsCI | GGATG | 2 cut(s) | 28, 258 |
| Cac8I | GCNNGC | 1 cut(s) | 117 |
| Cfr13I | GGNCC | 1 cut(s) | 72 |
| CviAII | CATG | 2 cut(s) | 53, 284 |
| CviJI | RGCY | 4 cut(s) | 95, 125, 170, 181 |
| CviKI_1 | RGCY | 4 cut(s) | 95, 125, 170, 181 |
| DdeI | CTNAG | 1 cut(s) | 57 |
| DpnI | GATC | 1 cut(s) | 6 |
| DpnII | GATC | 1 cut(s) | 4 |
| DraI | TTTAAA | 1 cut(s) | 291 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 217 |
| DriI | GACNNNNNGTC | 1 cut(s) | 70 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 217 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 70 |
| Eco47I | GGWCC | 1 cut(s) | 72 |
| Eco57I | CTGAAG | 1 cut(s) | 141 |
| EcoRI | GAATTC | 1 cut(s) | 263 |
| EcoRII | CCWGG | 1 cut(s) | 142 |
| EcoT22I | ATGCAT | 2 cut(s) | 84, 189 |
| FaeI | CATG | 2 cut(s) | 56, 287 |
| FaiI | YATR | 8 cut(s) | 54, 80, 84, 105, 173, 230, 285, 333 |
| FatI | CATG | 2 cut(s) | 52, 283 |
| FokI | GGATG | 2 cut(s) | 35, 265 |
| Hin1II | CATG | 2 cut(s) | 56, 287 |
| HinfI | GANTC | 1 cut(s) | 29 |
| Hpy166II | GTNNAC | 1 cut(s) | 64 |
| Hpy188I | TCNGA | 1 cut(s) | 252 |
| Hpy188III | TCNNGA | 4 cut(s) | 8, 47, 235, 320 |
| Hpy8I | GTNNAC | 1 cut(s) | 64 |
| HpyCH4V | TGCA | 3 cut(s) | 82, 187, 192 |
| HpyF3I | CTNAG | 1 cut(s) | 57 |
| Hsp92II | CATG | 2 cut(s) | 56, 287 |
| Kzo9I | GATC | 1 cut(s) | 4 |
| LpnPI | CCDG | 4 cut(s) | 129, 156, 220, 244 |
| LweI | GCATC | 2 cut(s) | 174, 243 |
| MalI | GATC | 1 cut(s) | 6 |
| MboI | GATC | 1 cut(s) | 4 |
| MboII | GAAGA | 1 cut(s) | 130 |
| MluCI | AATT | 5 cut(s) | 110, 152, 263, 292, 340 |
| MmeI | TCCRAC | 1 cut(s) | 230 |
| MnlI | CCTC | 3 cut(s) | 10, 52, 209 |
| Mph1103I | ATGCAT | 2 cut(s) | 84, 189 |
| MseI | TTAA | 2 cut(s) | 164, 290 |
| MspR9I | CCNGG | 1 cut(s) | 144 |
| MvaI | CCWGG | 1 cut(s) | 144 |
| NdeII | GATC | 1 cut(s) | 4 |
| NlaIII | CATG | 2 cut(s) | 56, 287 |
| NlaIV | GGNNCC | 2 cut(s) | 73, 148 |
| NsiI | ATGCAT | 2 cut(s) | 84, 189 |
| NspI | RCATGY | 1 cut(s) | 56 |
| PfeI | GAWTC | 1 cut(s) | 29 |
| Psp6I | CCWGG | 1 cut(s) | 142 |
| PspGI | CCWGG | 1 cut(s) | 142 |
| PspN4I | GGNNCC | 2 cut(s) | 73, 148 |
| PspPI | GGNCC | 1 cut(s) | 72 |
| SaqAI | TTAA | 2 cut(s) | 164, 290 |
| Sau3AI | GATC | 1 cut(s) | 4 |
| Sau96I | GGNCC | 1 cut(s) | 72 |
| ScrFI | CCNGG | 1 cut(s) | 144 |
| SetI | ASST | 4 cut(s) | 63, 97, 127, 183 |
| SfaNI | GCATC | 2 cut(s) | 174, 243 |
| SinI | GGWCC | 1 cut(s) | 72 |
| Sse9I | AATT | 5 cut(s) | 110, 152, 263, 292, 340 |
| StyD4I | CCNGG | 1 cut(s) | 142 |
| TasI | AATT | 5 cut(s) | 110, 152, 263, 292, 340 |
| TfiI | GAWTC | 1 cut(s) | 29 |
| Tru1I | TTAA | 2 cut(s) | 164, 290 |
| Tru9I | TTAA | 2 cut(s) | 164, 290 |
| TspDTI | ATGAA | 2 cut(s) | 21, 120 |
| VpaK11BI | GGWCC | 1 cut(s) | 72 |
| XapI | RAATTY | 2 cut(s) | 263, 292 |
| XceI | RCATGY | 1 cut(s) | 56 |
| Zsp2I | ATGCAT | 2 cut(s) | 84, 189 |