pycom13g11050

TdcA1-ORF2 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001541
Physical Location & Seq
Forward (+)
7529992 .. 7530975
984 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g11050.1

Sequence Viewer

Length: 738 bp
ATGCAAGTAGCTCATTGGTTCATACTTAACAACTGTGACGAGATAATTGAGTACCTGGATGAGTATGAAGAGTTGATGAAGCGGAAGTATCCTTCACATTTATATGCCGTGAAGCACAGTGACTTGTTTCCTCATTGGTTTCGCGAGCAAGTGAAAATAATGAAGGAAACCAATTCCCCTACGTATACTGAGGAATCATACAATTTGGCTATCGGACCTCTAAGCATCGAACTGTACTCATGGTGTCATGTGAACGGCATAAAATTCTTGGGAGCTGAGCGTGATGACAAGCTTTACCCTAGTAGGAATGAGAGTGTTAAACGGGATGATGGTTTACTGTCGGTGAACACTAACCGATGTTGGTACAGTGAAGATCGATACATTTTGGCAACTATGGCCAAGCAAATTTTCTACATCGATGACCCAAAGGCCAGGAGGGGATGGAAAGTTGTTCAGAGGATTGATCACAAAAGGGTGTATGATATTATGAACCGAGATCCTGACGACAACGACGATGACGACAACTTTGCTGACCAACTCCTCGAAACTTCGACACAAATTGGTGCAGAAACACTTCGAGATACTAATATTGTCCAAGAACCATTTCAGGTAGCTGGAGGTCCTCAAATCAAAATTCCTATAGACTCAATTACAATTGATTTCAAAAGTCTCCCAATATACAATCCACCTGAGGGCACATATGATGTTTTTGATGTACCAATGGATCAGGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transpos_assoc Transposase_21 Transposase_24

Protein Analysis

246

Amino Acids

28.8

Weight (kDa)

4.68

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4216 PF13952 102 - 151 4e-11 Domain of unknown function (DUF4216)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 185
AccII CGCG 1 cut(s) 144
AciI CCGC 1 cut(s) 82
AclWI GGATC 2 cut(s) 491, 732
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 3 cut(s) 263, 405, 633
AfaI GTAC 4 cut(s) 53, 236, 365, 717
AfiI CCNNNNNNNGG 1 cut(s) 692
AgsI TTSAA 1 cut(s) 664
AjnI CCWGG 2 cut(s) 54, 431
AjuI GAANNNNNNNTTGG 4 cut(s) 392, 424, 588, 620
AluBI AGCT 4 cut(s) 11, 275, 292, 614
AluI AGCT 4 cut(s) 11, 275, 292, 614
Alw26I GTCTC 1 cut(s) 674
AlwI GGATC 2 cut(s) 491, 732
AoxI GGCC 2 cut(s) 396, 429
ApoI RAATTY 3 cut(s) 263, 405, 633
ArsI GACNNNNNNTTYG 2 cut(s) 509, 541
Asp700I GAANNNNTTC 2 cut(s) 573, 603
AspS9I GGNCC 2 cut(s) 215, 620
AsuHPI GGTGA 1 cut(s) 355
AvaII GGWCC 2 cut(s) 215, 620
AxyI CCTNAGG 1 cut(s) 690
BaeGI GKGCMC 1 cut(s) 698
BalI TGGCCA 1 cut(s) 398
BccI CCATC 2 cut(s) 323, 435
BceAI ACGGC 2 cut(s) 92, 271
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BciT130I CCWGG 2 cut(s) 56, 433
BciVI GTATCC 1 cut(s) 99
BclI TGATCA 1 cut(s) 463
BcoDI GTCTC 1 cut(s) 674
BfaI CTAG 1 cut(s) 300
BfmI CTRYAG 1 cut(s) 639
BfuI GTATCC 1 cut(s) 99
BlpI GCTNAGC 1 cut(s) 276
Bme1390I CCNGG 2 cut(s) 56, 433
Bme18I GGWCC 2 cut(s) 215, 620
BmgT120I GGNCC 2 cut(s) 215, 620
BmrFI CCNGG 2 cut(s) 56, 433
BmsI GCATC 1 cut(s) 234
BpmI CTGGAG 1 cut(s) 636
Bpu1102I GCTNAGC 1 cut(s) 276
Bsa29I ATCGAT 2 cut(s) 376, 417
BsaAI YACGTR 1 cut(s) 183
Bsc4I CCNNNNNNNGG 1 cut(s) 692
Bse21I CCTNAGG 1 cut(s) 690
BseBI CCWGG 2 cut(s) 56, 433
BseCI ATCGAT 2 cut(s) 376, 417
BseGI GGATG 4 cut(s) 64, 331, 446, 736
BseLI CCNNNNNNNGG 1 cut(s) 692
BseMII CTCAG 3 cut(s) 180, 267, 681
BseRI GAGGAG 1 cut(s) 530
BseSI GKGCMC 1 cut(s) 698
BsgI GTGCAG 1 cut(s) 585
Bsh1236I CGCG 1 cut(s) 144
BshFI GGCC 2 cut(s) 398, 431
BshVI ATCGAT 2 cut(s) 376, 417
BslI CCNNNNNNNGG 1 cut(s) 692
BsmAI GTCTC 1 cut(s) 674
BsnI GGCC 2 cut(s) 398, 431
Bsp1286I GDGCHC 1 cut(s) 698
Bsp143I GATC 4 cut(s) 373, 463, 496, 724
Bsp1720I GCTNAGC 1 cut(s) 276
Bsp68I TCGCGA 1 cut(s) 144
BspACI CCGC 1 cut(s) 82
BspANI GGCC 2 cut(s) 398, 431
BspCNI CTCAG 3 cut(s) 181, 268, 682
BspDI ATCGAT 2 cut(s) 376, 417
BspFNI CGCG 1 cut(s) 144
BspPI GGATC 2 cut(s) 491, 732
BssMI GATC 4 cut(s) 373, 463, 496, 724
BssNAI GTATAC 1 cut(s) 186
Bst1107I GTATAC 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 56, 433
Bst4CI ACNGT 5 cut(s) 35, 119, 234, 339, 368
Bst6I CTCTTC 1 cut(s) 63
BstBAI YACGTR 1 cut(s) 183
BstC8I GCNNGC 1 cut(s) 146
BstDEI CTNAG 4 cut(s) 189, 221, 276, 690
BstF5I GGATG 4 cut(s) 64, 331, 446, 736
BstFNI CGCG 1 cut(s) 144
BstKTI GATC 4 cut(s) 376, 466, 499, 727
BstMAI GTCTC 1 cut(s) 674
BstMBI GATC 4 cut(s) 373, 463, 496, 724
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstNI CCWGG 2 cut(s) 56, 433
BstSCI CCNGG 2 cut(s) 54, 431
BstSFI CTRYAG 1 cut(s) 639
BstSLI GKGCMC 1 cut(s) 698
BstSNI TACGTA 1 cut(s) 183
BstUI CGCG 1 cut(s) 144
BstX2I RGATCY 1 cut(s) 496
BstYI RGATCY 1 cut(s) 496
BstZ17I GTATAC 1 cut(s) 186
Bsu15I ATCGAT 2 cut(s) 376, 417
Bsu36I CCTNAGG 1 cut(s) 690
BsuI GTATCC 1 cut(s) 99
BsuRI GGCC 2 cut(s) 398, 431
BsuTUI ATCGAT 2 cut(s) 376, 417
BtsCI GGATG 4 cut(s) 64, 331, 446, 736
BtsIMutI CAGTG 2 cut(s) 124, 373
BtuMI TCGCGA 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 146
Cfr13I GGNCC 2 cut(s) 215, 620
ClaI ATCGAT 2 cut(s) 376, 417
Csp6I GTAC 4 cut(s) 52, 235, 364, 716
CviAII CATG 2 cut(s) 240, 248
CviJI RGCY 7 cut(s) 11, 209, 275, 292, 398, 431, 614
CviKI_1 RGCY 7 cut(s) 11, 209, 275, 292, 398, 431, 614
CviQI GTAC 4 cut(s) 52, 235, 364, 716
DdeI CTNAG 4 cut(s) 189, 221, 276, 690
DpnI GATC 4 cut(s) 375, 465, 498, 726
DpnII GATC 4 cut(s) 373, 463, 496, 724
EaeI YGGCCR 1 cut(s) 396
Eam1104I CTCTTC 1 cut(s) 63
EarI CTCTTC 1 cut(s) 63
Eco105I TACGTA 1 cut(s) 183
Eco47I GGWCC 2 cut(s) 215, 620
Eco81I CCTNAGG 1 cut(s) 690
EcoO109I RGGNCCY 1 cut(s) 620
EcoRII CCWGG 2 cut(s) 54, 431
FaeI CATG 2 cut(s) 243, 251
FatI CATG 2 cut(s) 239, 247
FauNDI CATATG 1 cut(s) 700
FbaI TGATCA 1 cut(s) 463
FblI GTMKAC 1 cut(s) 185
FokI GGATG 3 cut(s) 71, 338, 453
FspBI CTAG 1 cut(s) 300
GsuI CTGGAG 1 cut(s) 636
HaeIII GGCC 2 cut(s) 398, 431
Hin1II CATG 2 cut(s) 243, 251
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 2 cut(s) 194, 644
HphI GGTGA 1 cut(s) 355
Hpy166II GTNNAC 4 cut(s) 186, 253, 335, 346
Hpy188I TCNGA 2 cut(s) 215, 456
Hpy188III TCNNGA 4 cut(s) 143, 500, 578, 728
Hpy8I GTNNAC 4 cut(s) 186, 253, 335, 346
Hpy99I CGWCG 1 cut(s) 515
HpyAV CCTTC 2 cut(s) 102, 157
HpyCH4III ACNGT 5 cut(s) 35, 119, 234, 339, 368
HpyCH4IV ACGT 1 cut(s) 182
HpyCH4V TGCA 2 cut(s) 4, 566
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 4 cut(s) 189, 221, 276, 690
HpySE526I ACGT 1 cut(s) 182
Hsp92II CATG 2 cut(s) 243, 251
Ksp22I TGATCA 1 cut(s) 463
Kzo9I GATC 4 cut(s) 373, 463, 496, 724
LmnI GCTCC 1 cut(s) 272
LpnPI CCDG 9 cut(s) 41, 68, 418, 445, 513, 593, 600, 702, 713
LweI GCATC 1 cut(s) 234
MaeI CTAG 1 cut(s) 300
MaeII ACGT 1 cut(s) 182
MaeIII GTNAC 2 cut(s) 35, 119
MalI GATC 4 cut(s) 375, 465, 498, 726
MboI GATC 4 cut(s) 373, 463, 496, 724
MboII GAAGA 2 cut(s) 80, 383
MfeI CAATTG 1 cut(s) 654
MflI RGATCY 1 cut(s) 496
MhlI GDGCHC 1 cut(s) 698
MlsI TGGCCA 1 cut(s) 398
MluCI AATT 9 cut(s) 45, 172, 202, 263, 405, 558, 633, 648, 654
MluNI TGGCCA 1 cut(s) 398
MlyI GAGTC 1 cut(s) 638
MnlI CCTC 9 cut(s) 141, 184, 228, 429, 450, 551, 611, 633, 685
Mox20I TGGCCA 1 cut(s) 398
MroXI GAANNNNTTC 2 cut(s) 573, 603
MscI TGGCCA 1 cut(s) 398
MseI TTAA 2 cut(s) 27, 318
MslI CAYNNNNRTG 1 cut(s) 102
Msp20I TGGCCA 1 cut(s) 398
MspR9I CCNGG 2 cut(s) 56, 433
MunI CAATTG 1 cut(s) 654
MvaI CCWGG 2 cut(s) 56, 433
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 1 cut(s) 395
NdeI CATATG 1 cut(s) 700
NdeII GATC 4 cut(s) 373, 463, 496, 724
NlaIII CATG 2 cut(s) 243, 251
NmuCI GTSAC 2 cut(s) 35, 119
NruI TCGCGA 1 cut(s) 144
PcsI WCGNNNNNNNCGW 2 cut(s) 510, 516
PdmI GAANNNNTTC 2 cut(s) 573, 603
PfeI GAWTC 1 cut(s) 194
PleI GAGTC 1 cut(s) 638
PpsI GAGTC 1 cut(s) 638
Ppu21I YACGTR 1 cut(s) 183
PpuMI RGGWCCY 1 cut(s) 620
Psp5II RGGWCCY 1 cut(s) 620
Psp6I CCWGG 2 cut(s) 54, 431
PspGI CCWGG 2 cut(s) 54, 431
PspPI GGNCC 2 cut(s) 215, 620
PspPPI RGGWCCY 1 cut(s) 620
PsuI RGATCY 1 cut(s) 496
RruI TCGCGA 1 cut(s) 144
RsaI GTAC 4 cut(s) 53, 236, 365, 717
RsaNI GTAC 4 cut(s) 52, 235, 364, 716
RseI CAYNNNNRTG 1 cut(s) 102
SaqAI TTAA 2 cut(s) 27, 318
Sau3AI GATC 4 cut(s) 373, 463, 496, 724
Sau96I GGNCC 2 cut(s) 215, 620
SchI GAGTC 1 cut(s) 638
ScrFI CCNGG 2 cut(s) 56, 433
SduI GDGCHC 1 cut(s) 698
SfaNI GCATC 1 cut(s) 234
SfcI CTRYAG 1 cut(s) 639
SinI GGWCC 2 cut(s) 215, 620
SmiMI CAYNNNNRTG 1 cut(s) 102
SnaBI TACGTA 1 cut(s) 183
Sse9I AATT 9 cut(s) 45, 172, 202, 263, 405, 558, 633, 648, 654
SsiI CCGC 1 cut(s) 82
SspI AATATT 1 cut(s) 589
SspMI CTAG 1 cut(s) 300
StyD4I CCNGG 2 cut(s) 54, 431
TaaI ACNGT 5 cut(s) 35, 119, 234, 339, 368
TaiI ACGT 1 cut(s) 185
TaqI TCGA 6 cut(s) 228, 376, 417, 543, 551, 577
TasI AATT 9 cut(s) 45, 172, 202, 263, 405, 558, 633, 648, 654
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 1 cut(s) 194
Tru1I TTAA 2 cut(s) 27, 318
Tru9I TTAA 2 cut(s) 27, 318
TscAI CASTG 2 cut(s) 124, 373
TseFI GTSAC 2 cut(s) 35, 119
Tsp45I GTSAC 2 cut(s) 35, 119
TspDTI ATGAA 5 cut(s) 10, 81, 92, 176, 503
TspRI CASTG 2 cut(s) 124, 373
VpaK11BI GGWCC 2 cut(s) 215, 620
XapI RAATTY 3 cut(s) 263, 405, 633
XmiI GTMKAC 1 cut(s) 185
XmnI GAANNNNTTC 2 cut(s) 573, 603
XspI CTAG 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.