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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 1 cut(s) | 53 |
| Ama87I | CYCGRG | 1 cut(s) | 132 |
| AvaI | CYCGRG | 1 cut(s) | 132 |
| BccI | CCATC | 1 cut(s) | 137 |
| BfaI | CTAG | 1 cut(s) | 98 |
| BmeT110I | CYCGRG | 1 cut(s) | 132 |
| Bsa29I | ATCGAT | 1 cut(s) | 84 |
| BsaBI | GATNNNNATC | 1 cut(s) | 90 |
| Bse8I | GATNNNNATC | 1 cut(s) | 90 |
| BseCI | ATCGAT | 1 cut(s) | 84 |
| BseJI | GATNNNNATC | 1 cut(s) | 90 |
| BshVI | ATCGAT | 1 cut(s) | 84 |
| BsiHKCI | CYCGRG | 1 cut(s) | 132 |
| BsoBI | CYCGRG | 1 cut(s) | 132 |
| BspDI | ATCGAT | 1 cut(s) | 84 |
| Bsu15I | ATCGAT | 1 cut(s) | 84 |
| BsuTUI | ATCGAT | 1 cut(s) | 84 |
| ClaI | ATCGAT | 1 cut(s) | 84 |
| Eco88I | CYCGRG | 1 cut(s) | 132 |
| FaiI | YATR | 1 cut(s) | 65 |
| FspBI | CTAG | 1 cut(s) | 98 |
| FspEI | CC | 6 cut(s) | 15, 33, 86, 99, 108, 129 |
| Hpy188III | TCNNGA | 3 cut(s) | 98, 114, 134 |
| LpnPI | CCDG | 2 cut(s) | 33, 99 |
| MaeI | CTAG | 1 cut(s) | 98 |
| MaeIII | GTNAC | 1 cut(s) | 68 |
| MboII | GAAGA | 1 cut(s) | 134 |
| MluCI | AATT | 2 cut(s) | 24, 104 |
| MnlI | CCTC | 2 cut(s) | 94, 105 |
| NmuCI | GTSAC | 1 cut(s) | 68 |
| PaeR7I | CTCGAG | 1 cut(s) | 132 |
| SetI | ASST | 1 cut(s) | 22 |
| Sfr274I | CTCGAG | 1 cut(s) | 132 |
| SgeI | CNNG | 5 cut(s) | 32, 110, 126, 144, 146 |
| SlaI | CTCGAG | 1 cut(s) | 132 |
| SmlI | CTYRAG | 1 cut(s) | 132 |
| SmoI | CTYRAG | 1 cut(s) | 132 |
| Sse9I | AATT | 2 cut(s) | 24, 104 |
| SspMI | CTAG | 1 cut(s) | 98 |
| TaqI | TCGA | 3 cut(s) | 15, 84, 133 |
| TasI | AATT | 2 cut(s) | 24, 104 |
| TseFI | GTSAC | 1 cut(s) | 68 |
| Tsp45I | GTSAC | 1 cut(s) | 68 |
| TspDTI | ATGAA | 2 cut(s) | 17, 135 |
| XbaI | TCTAGA | 1 cut(s) | 97 |
| XhoI | CTCGAG | 1 cut(s) | 132 |
| XspI | CTAG | 1 cut(s) | 98 |