Rmu_sc0008079.1_g000012

Domain of unknown function (DUF4216)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008079.1
OG0001541
Physical Location & Seq
Forward (+)
70926 .. 78412
7487 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008079.1_g000012.1.cds

Sequence Viewer

Length: 1035 bp
atggagatctccggattcaagtacgtagatatgcatcacgtttttctattcaaatgcaagtggttcaacacagacgaaacagagagagacgcgagaaaaatagtacaacgtgattataatcttatgagcattaattcaagctttgtttggtatgaggatgaaccttttatacttgctagtgaagcgcatcaagtgttttatttagatgattataagttgggccctgaatggaaagttgtccaaaaagtgcaaatgagacatgtatgggatgtcccagaggtagatgacgaggatgaagaggtagatgaggaaaattttgatccagtggctaataatgatgtatatcagcaagatgaattcaatgaaattcaatggtcagttcaagaagagggtcttgaaaatcctcaattacatcaagaggatattgagcccgaagtcattgcttcaaatgtcgagagtgacgaatggatagatgatgaaggtccagaaaaaaaagtgtgtggaaagacaagaggtttaaatgctgataaggtgcatgagcagcttggtactaaaatacctgtgactttcaagaaagaaaatggcagacccacaggtccatatgcagaaatgtttgctaatgaagttggattcaccattcggaatcatgcacctctaaatgtgaaaaaatggaaggaagtaaaaccagaagataggacaagtttgatcaaaagaataactactaagtacgacattgacatgtctctaccttgggttaagagatatgtgaataaatcttttggaacagtctccgatctgccggatttgaggccgtcccggtggaaaaggttacgtccagcagctaggggaggtcgagacgaagctgtggtggctcaagggtggcttggagttgatcaagttagtgattgcagcatcaccaagagcgccatcaccatgatggatgccatccatggcatcatggatagggactatgccagccctaggctggtggtggacggaggcggtgccctaggcagcaacgtcggtcacacttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218

Protein Analysis

344

Amino Acids

39.61

Weight (kDa)

4.85

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 117, 213
AasI GACNNNNNNGTC 1 cut(s) 594
AccB1I GGYRCC 1 cut(s) 1004
AccII CGCG 1 cut(s) 92
AccIII TCCGGA 1 cut(s) 11
AciI CCGC 1 cut(s) 1002
AclWI GGATC 1 cut(s) 314
AcsI RAATTY 3 cut(s) 313, 356, 366
AfaI GTAC 4 cut(s) 23, 105, 550, 728
AfiI CCNNNNNNNGG 2 cut(s) 981, 985
AflIII ACRYGT 2 cut(s) 259, 738
AluBI AGCT 4 cut(s) 141, 544, 842, 863
AluI AGCT 4 cut(s) 141, 544, 842, 863
Alw26I GTCTC 5 cut(s) 81, 250, 747, 793, 849
AlwI GGATC 1 cut(s) 314
Aor13HI TCCGGA 1 cut(s) 11
AoxI GGCC 2 cut(s) 220, 809
ApaI GGGCCC 1 cut(s) 224
ApeKI GCWGC 4 cut(s) 541, 839, 909, 1014
ApoI RAATTY 3 cut(s) 313, 356, 366
AseI ATTAAT 1 cut(s) 132
AspA2I CCTAGG 2 cut(s) 980, 1009
AspLEI GCGC 2 cut(s) 187, 926
AspS9I GGNCC 4 cut(s) 220, 221, 482, 596
AsuC2I CCSGG 1 cut(s) 817
AsuHPI GGTGA 3 cut(s) 625, 907, 922
AvaII GGWCC 2 cut(s) 482, 596
AvrII CCTAGG 2 cut(s) 980, 1009
BaeGI GKGCMC 2 cut(s) 224, 1009
BanI GGYRCC 1 cut(s) 1004
BanII GRGCYC 2 cut(s) 224, 432
BbvI GCAGC 4 cut(s) 553, 851, 921, 1026
BccI CCATC 3 cut(s) 931, 935, 953
BceAI ACGGC 1 cut(s) 796
BcgI CGANNNNNNTGC 3 cut(s) 422, 456, 1003
BclI TGATCA 2 cut(s) 705, 892
BcnI CCSGG 1 cut(s) 817
BcoDI GTCTC 5 cut(s) 81, 250, 747, 793, 849
BfaI CTAG 4 cut(s) 177, 843, 981, 1010
BfoI RGCGCY 1 cut(s) 927
BglII AGATCT 1 cut(s) 6
BisI GCNGC 4 cut(s) 542, 840, 910, 1015
BlnI CCTAGG 2 cut(s) 980, 1009
BlsI GCNGC 4 cut(s) 543, 841, 911, 1016
Bme1390I CCNGG 1 cut(s) 817
Bme18I GGWCC 2 cut(s) 482, 596
BmgT120I GGNCC 4 cut(s) 220, 221, 482, 596
BmiI GGNNCC 2 cut(s) 222, 1006
BmrFI CCNGG 1 cut(s) 817
BmsI GCATC 5 cut(s) 43, 196, 921, 931, 963
BpuEI CTTGAG 1 cut(s) 858
BpuMI CCSGG 1 cut(s) 817
BsaAI YACGTR 1 cut(s) 25
BsaBI GATNNNNATC 3 cut(s) 33, 117, 342
BsaJI CCNNGG 4 cut(s) 749, 949, 980, 1009
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 2 cut(s) 981, 985
Bse1I ACTGG 1 cut(s) 323
Bse3DI GCAATG 1 cut(s) 438
Bse8I GATNNNNATC 3 cut(s) 33, 117, 342
BseAI TCCGGA 1 cut(s) 11
BseDI CCNNGG 4 cut(s) 749, 949, 980, 1009
BseGI GGATG 5 cut(s) 163, 274, 298, 945, 946
BseJI GATNNNNATC 3 cut(s) 33, 117, 342
BseLI CCNNNNNNNGG 2 cut(s) 981, 985
BseMI GCAATG 1 cut(s) 438
BseNI ACTGG 1 cut(s) 323
BseSI GKGCMC 2 cut(s) 224, 1009
BseXI GCAGC 4 cut(s) 553, 851, 921, 1026
Bsh1236I CGCG 1 cut(s) 92
BshFI GGCC 2 cut(s) 222, 811
BshNI GGYRCC 1 cut(s) 1004
BsiSI CCGG 3 cut(s) 12, 800, 817
BslFI GGGAC 3 cut(s) 257, 799, 980
BslI CCNNNNNNNGG 2 cut(s) 981, 985
BsmAI GTCTC 5 cut(s) 81, 250, 747, 793, 849
BsmBI CGTCTC 2 cut(s) 81, 849
BsmFI GGGAC 3 cut(s) 257, 799, 980
BsnI GGCC 2 cut(s) 222, 811
Bsp120I GGGCCC 1 cut(s) 220
Bsp1286I GDGCHC 3 cut(s) 224, 432, 1009
Bsp13I TCCGGA 1 cut(s) 11
Bsp143I GATC 5 cut(s) 6, 319, 705, 793, 892
Bsp19I CCATGG 1 cut(s) 949
BspACI CCGC 1 cut(s) 1002
BspANI GGCC 2 cut(s) 222, 811
BspEI TCCGGA 1 cut(s) 11
BspFNI CGCG 1 cut(s) 92
BspLI GGNNCC 2 cut(s) 222, 1006
BspPI GGATC 1 cut(s) 314
BspT107I GGYRCC 1 cut(s) 1004
BsrDI GCAATG 1 cut(s) 438
BsrI ACTGG 1 cut(s) 323
BssECI CCNNGG 4 cut(s) 749, 949, 980, 1009
BssMI GATC 5 cut(s) 6, 319, 705, 793, 892
BssT1I CCWWGG 4 cut(s) 749, 949, 980, 1009
Bst4CI ACNGT 1 cut(s) 787
Bst6I CTCTTC 2 cut(s) 291, 381
BstBAI YACGTR 1 cut(s) 25
BstC8I GCNNGC 1 cut(s) 976
BstDEI CTNAG 2 cut(s) 723, 1032
BstDSI CCRYGG 1 cut(s) 949
BstF5I GGATG 5 cut(s) 163, 274, 298, 945, 946
BstFNI CGCG 1 cut(s) 92
BstH2I RGCGCY 1 cut(s) 927
BstHHI GCGC 2 cut(s) 187, 926
BstKTI GATC 5 cut(s) 9, 322, 708, 796, 895
BstMAI GTCTC 5 cut(s) 81, 250, 747, 793, 849
BstMBI GATC 5 cut(s) 6, 319, 705, 793, 892
BstMWI GCNNNNNNNGC 3 cut(s) 182, 541, 869
BstNSI RCATGY 2 cut(s) 263, 742
BstSCI CCNGG 1 cut(s) 815
BstSLI GKGCMC 2 cut(s) 224, 1009
BstSNI TACGTA 1 cut(s) 25
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 4 cut(s) 553, 851, 921, 1026
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
BsuRI GGCC 2 cut(s) 222, 811
BtgI CCRYGG 1 cut(s) 949
BtsCI GGATG 5 cut(s) 163, 274, 298, 945, 946
BtsIMutI CAGTG 1 cut(s) 330
Cac8I GCNNGC 1 cut(s) 976
CfoI GCGC 2 cut(s) 187, 926
Cfr13I GGNCC 4 cut(s) 220, 221, 482, 596
CseI GACGC 1 cut(s) 98
Csp6I GTAC 4 cut(s) 22, 104, 549, 727
CviAII CATG 7 cut(s) 260, 536, 647, 739, 934, 950, 958
CviQI GTAC 4 cut(s) 22, 104, 549, 727
DdeI CTNAG 2 cut(s) 723, 1032
DpnI GATC 5 cut(s) 8, 321, 707, 795, 894
DpnII GATC 5 cut(s) 6, 319, 705, 793, 892
DraI TTTAAA 1 cut(s) 519
DrdI GACNNNNNNGTC 1 cut(s) 594
DseDI GACNNNNNNGTC 1 cut(s) 594
Eam1104I CTCTTC 2 cut(s) 291, 381
EarI CTCTTC 2 cut(s) 291, 381
Eco105I TACGTA 1 cut(s) 25
Eco130I CCWWGG 4 cut(s) 749, 949, 980, 1009
Eco24I GRGCYC 2 cut(s) 224, 432
Eco47I GGWCC 2 cut(s) 482, 596
EcoO109I RGGNCCY 1 cut(s) 221
EcoRI GAATTC 1 cut(s) 356
EcoT14I CCWWGG 4 cut(s) 749, 949, 980, 1009
EcoT22I ATGCAT 1 cut(s) 36
EcoT38I GRGCYC 2 cut(s) 224, 432
ErhI CCWWGG 4 cut(s) 749, 949, 980, 1009
Esp3I CGTCTC 2 cut(s) 81, 849
FaeI CATG 7 cut(s) 263, 539, 650, 742, 937, 953, 961
FalI AAGNNNNNCTT 4 cut(s) 378, 410, 867, 899
FaqI GGGAC 3 cut(s) 257, 799, 980
FatI CATG 7 cut(s) 259, 535, 646, 738, 933, 949, 957
FauNDI CATATG 1 cut(s) 601
FbaI TGATCA 2 cut(s) 705, 892
Fnu4HI GCNGC 4 cut(s) 542, 840, 910, 1015
FokI GGATG 5 cut(s) 170, 281, 305, 932, 953
FriOI GRGCYC 2 cut(s) 224, 432
Fsp4HI GCNGC 4 cut(s) 542, 840, 910, 1015
FspBI CTAG 4 cut(s) 177, 843, 981, 1010
GlaI GCGC 2 cut(s) 186, 925
GluI GCNGC 4 cut(s) 542, 840, 910, 1015
HaeII RGCGCY 1 cut(s) 927
HaeIII GGCC 2 cut(s) 222, 811
HapII CCGG 3 cut(s) 12, 800, 817
HgaI GACGC 1 cut(s) 98
HhaI GCGC 2 cut(s) 187, 926
Hin1II CATG 7 cut(s) 263, 539, 650, 742, 937, 953, 961
Hin6I GCGC 2 cut(s) 185, 924
HinP1I GCGC 2 cut(s) 185, 924
HindIII AAGCTT 1 cut(s) 139
HinfI GANTC 3 cut(s) 15, 630, 643
HpaII CCGG 3 cut(s) 12, 800, 817
HphI GGTGA 3 cut(s) 625, 907, 922
Hpy166II GTNNAC 1 cut(s) 994
Hpy188I TCNGA 2 cut(s) 642, 793
Hpy188III TCNNGA 8 cut(s) 12, 383, 395, 416, 454, 485, 571, 854
Hpy8I GTNNAC 1 cut(s) 994
Hpy99I CGWCG 1 cut(s) 1025
HpyAV CCTTC 2 cut(s) 473, 667
HpyCH4III ACNGT 1 cut(s) 787
HpyCH4IV ACGT 5 cut(s) 24, 39, 109, 832, 1020
HpyCH4V TGCA 7 cut(s) 34, 57, 250, 535, 605, 650, 909
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 541, 869
HpyF3I CTNAG 2 cut(s) 723, 1032
HpySE526I ACGT 5 cut(s) 24, 39, 109, 832, 1020
Hsp92II CATG 7 cut(s) 263, 539, 650, 742, 937, 953, 961
HspAI GCGC 2 cut(s) 185, 924
Kpn2I TCCGGA 1 cut(s) 11
Ksp22I TGATCA 2 cut(s) 705, 892
Kzo9I GATC 5 cut(s) 6, 319, 705, 793, 892
Lsp1109I GCAGC 4 cut(s) 553, 851, 921, 1026
LweI GCATC 5 cut(s) 43, 196, 921, 931, 963
MaeI CTAG 4 cut(s) 177, 843, 981, 1010
MaeII ACGT 5 cut(s) 24, 39, 109, 832, 1020
MaeIII GTNAC 4 cut(s) 458, 562, 828, 1025
MalI GATC 5 cut(s) 8, 321, 707, 795, 894
MboI GATC 5 cut(s) 6, 319, 705, 793, 892
MboII GAAGA 3 cut(s) 308, 398, 701
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 3 cut(s) 224, 432, 1009
MluCI AATT 5 cut(s) 133, 313, 356, 366, 407
MmeI TCCRAC 1 cut(s) 607
Mph1103I ATGCAT 1 cut(s) 36
MroI TCCGGA 1 cut(s) 11
MseI TTAA 3 cut(s) 132, 518, 756
MslI CAYNNNNRTG 3 cut(s) 737, 932, 935
MspI CCGG 3 cut(s) 12, 800, 817
MspR9I CCNGG 1 cut(s) 817
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 3 cut(s) 182, 541, 869
NciI CCSGG 1 cut(s) 817
NcoI CCATGG 1 cut(s) 949
NdeI CATATG 1 cut(s) 601
NdeII GATC 5 cut(s) 6, 319, 705, 793, 892
NlaIII CATG 7 cut(s) 263, 539, 650, 742, 937, 953, 961
NlaIV GGNNCC 2 cut(s) 222, 1006
NmuCI GTSAC 3 cut(s) 458, 562, 1025
NsiI ATGCAT 1 cut(s) 36
NspI RCATGY 2 cut(s) 263, 742
PciI ACATGT 2 cut(s) 259, 738
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PfeI GAWTC 3 cut(s) 15, 630, 643
PkrI GCNGC 4 cut(s) 543, 841, 911, 1016
Ppu21I YACGTR 1 cut(s) 25
PscI ACATGT 2 cut(s) 259, 738
PshBI ATTAAT 1 cut(s) 132
PsiI TTATAA 2 cut(s) 117, 213
PspN4I GGNNCC 2 cut(s) 222, 1006
PspOMI GGGCCC 1 cut(s) 220
PspPI GGNCC 4 cut(s) 220, 221, 482, 596
PsrI GAACNNNNNNTAC 2 cut(s) 153, 185
PsuI RGATCY 1 cut(s) 6
RsaI GTAC 4 cut(s) 23, 105, 550, 728
RsaNI GTAC 4 cut(s) 22, 104, 549, 727
RseI CAYNNNNRTG 3 cut(s) 737, 932, 935
SaqAI TTAA 3 cut(s) 132, 518, 756
SatI GCNGC 4 cut(s) 542, 840, 910, 1015
Sau3AI GATC 5 cut(s) 6, 319, 705, 793, 892
Sau96I GGNCC 4 cut(s) 220, 221, 482, 596
ScrFI CCNGG 1 cut(s) 817
SduI GDGCHC 3 cut(s) 224, 432, 1009
SfaNI GCATC 5 cut(s) 43, 196, 921, 931, 963
SinI GGWCC 2 cut(s) 482, 596
SmiMI CAYNNNNRTG 3 cut(s) 737, 932, 935
SmlI CTYRAG 1 cut(s) 873
SmoI CTYRAG 1 cut(s) 873
SnaBI TACGTA 1 cut(s) 25
Sse9I AATT 5 cut(s) 133, 313, 356, 366, 407
SsiI CCGC 1 cut(s) 1002
SspMI CTAG 4 cut(s) 177, 843, 981, 1010
StyD4I CCNGG 1 cut(s) 815
StyI CCWWGG 4 cut(s) 749, 949, 980, 1009
TaaI ACNGT 1 cut(s) 787
TaiI ACGT 5 cut(s) 27, 42, 112, 835, 1023
TaqI TCGA 2 cut(s) 453, 853
TaqII GACCGA 1 cut(s) 1013
TasI AATT 5 cut(s) 133, 313, 356, 366, 407
TatI WGTACW 1 cut(s) 103
TfiI GAWTC 3 cut(s) 15, 630, 643
Tru1I TTAA 3 cut(s) 132, 518, 756
Tru9I TTAA 3 cut(s) 132, 518, 756
TscAI CASTG 1 cut(s) 330
TseFI GTSAC 3 cut(s) 458, 562, 1025
TseI GCWGC 4 cut(s) 541, 839, 909, 1014
Tsp45I GTSAC 3 cut(s) 458, 562, 1025
TspDTI ATGAA 6 cut(s) 174, 309, 369, 378, 492, 636
TspGWI ACGGA 1 cut(s) 1011
TspRI CASTG 1 cut(s) 330
VpaK11BI GGWCC 2 cut(s) 482, 596
VspI ATTAAT 1 cut(s) 132
XapI RAATTY 3 cut(s) 313, 356, 366
XceI RCATGY 2 cut(s) 263, 742
XcmI CCANNNNNNNNNTGG 2 cut(s) 934, 982
XmaJI CCTAGG 2 cut(s) 980, 1009
XspI CTAG 4 cut(s) 177, 843, 981, 1010
Zsp2I ATGCAT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.