RchiOBHm_Chr2g0106121

Domain of unknown function (DUF4216)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001541
Physical Location & Seq
Forward (+)
17531628 .. 17537656
6029 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48023

Sequence Viewer

Length: 903 bp
ATGGACCGAAGTTGGATAGAACACGAGGCTGAACTAAAGCAAACAAGTAATGCCAATTTATATAAAAGACAAGAAGAAGAGTTTCCTACTTGGTTTGAAAAACGAATGAGTCACTTAGTCCAAAGTGGATTGGTGGATGCAACTGATCAACTTTATTGTTTGGCTTGTAAACCCGATGTGCGTGCCCATACATATACTGGTTGTATTGTTGATGGGGTTAGGTTCCACACAAAAGATCTAGATGATCGTCGCACCACACAAAATAGTGGTATATATGTCCCTGGAGTTTTTGTTGGTGGAACACATGATTATTATGGTGTTTTGCTAAATATTGTGAGATTAAGGTACGTAGATATGCATCACATTTTTCTATTCAAATGCAAGTGGTTCAACACAGACGAAACAAAGAGAGACGCGAGAAAAAGAGTACAACGTGATTATAATCTTATGAGCATTAATTCAAGTTTTGTCTGGTATGAGGATGAACCTTTTATACTTGCTAGTGAAGCGCATCAAGTGTTTTATTTAGATGATTATAAGTTGGGCCCTGAATGGAAAGTTGTCCAAAAAGTGCAAATGAGACATGTATGGGATGTCCCAGAGGTAGATGACGAGGATGAAGAGGTAGATGAGGAAAATTTTGATCCAGTCGTGGCTAATAATGATGTATATCAGCAAGATGAATTCAATGAAATTCAATGGTCAGTTCAAGAAGAGGGTCTTGAAAATCCTCAATTACATCAAGAGGATATTGAGCCTGAAGTCATTGCTTCAAATGTCGAGAGTGACGAATTGATAGATGATAGTAGGCTTAATGATTTCATCTGTGATGATGTTGAAGAAAACGATACTGATTATGATGACAATGAAGAAATCATTTCAAGTGATGACAATAGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transposase_21

Protein Analysis

300

Amino Acids

35.42

Weight (kDa)

4.3

Isoelectric Point (pI)

50.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4216 PF13952 113 - 188 1.8e-19 Domain of unknown function (DUF4216)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 441, 537
AccII CGCG 1 cut(s) 416
AclWI GGATC 1 cut(s) 638
AcsI RAATTY 3 cut(s) 637, 683, 693
AcuI CTGAAG 1 cut(s) 780
AfaI GTAC 2 cut(s) 347, 429
AflIII ACRYGT 1 cut(s) 583
AjnI CCWGG 1 cut(s) 280
Alw26I GTCTC 2 cut(s) 405, 574
AlwI GGATC 1 cut(s) 638
AoxI GGCC 1 cut(s) 544
ApaI GGGCCC 1 cut(s) 548
ApoI RAATTY 3 cut(s) 637, 683, 693
AseI ATTAAT 1 cut(s) 456
Asp700I GAANNNNTTC 1 cut(s) 81
AspLEI GCGC 1 cut(s) 511
AspS9I GGNCC 3 cut(s) 4, 544, 545
AvaII GGWCC 1 cut(s) 4
BaeGI GKGCMC 2 cut(s) 187, 548
BanII GRGCYC 1 cut(s) 548
BauI CACGAG 1 cut(s) 23
BccI CCATC 1 cut(s) 206
BcgI CGANNNNNNTGC 2 cut(s) 164, 198
BciT130I CCWGG 1 cut(s) 282
BclI TGATCA 1 cut(s) 145
BcoDI GTCTC 2 cut(s) 405, 574
BfaI CTAG 2 cut(s) 239, 501
BglII AGATCT 1 cut(s) 235
Bme1390I CCNGG 1 cut(s) 282
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 3 cut(s) 4, 544, 545
BmiI GGNNCC 2 cut(s) 224, 546
BmrFI CCNGG 1 cut(s) 282
BmsI GCATC 3 cut(s) 127, 367, 520
BpmI CTGGAG 1 cut(s) 303
BsaAI YACGTR 1 cut(s) 349
BsaBI GATNNNNATC 3 cut(s) 357, 441, 669
BsaJI CCNNGG 1 cut(s) 280
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bse1I ACTGG 2 cut(s) 202, 647
Bse3DI GCAATG 1 cut(s) 765
Bse8I GATNNNNATC 3 cut(s) 357, 441, 669
BseBI CCWGG 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 4 cut(s) 142, 487, 598, 622
BseJI GATNNNNATC 3 cut(s) 357, 441, 669
BseMI GCAATG 1 cut(s) 765
BseNI ACTGG 2 cut(s) 202, 647
BseSI GKGCMC 2 cut(s) 187, 548
Bsh1236I CGCG 1 cut(s) 416
BshFI GGCC 1 cut(s) 546
BslFI GGGAC 2 cut(s) 263, 581
BsmAI GTCTC 2 cut(s) 405, 574
BsmBI CGTCTC 1 cut(s) 405
BsmFI GGGAC 2 cut(s) 263, 581
BsnI GGCC 1 cut(s) 546
Bsp120I GGGCCC 1 cut(s) 544
Bsp1286I GDGCHC 2 cut(s) 187, 548
Bsp143I GATC 4 cut(s) 145, 235, 244, 643
BspANI GGCC 1 cut(s) 546
BspFNI CGCG 1 cut(s) 416
BspLI GGNNCC 2 cut(s) 224, 546
BspPI GGATC 1 cut(s) 638
BsrDI GCAATG 1 cut(s) 765
BsrI ACTGG 2 cut(s) 202, 647
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 4 cut(s) 145, 235, 244, 643
BssSI CACGAG 1 cut(s) 23
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 1 cut(s) 282
Bst6I CTCTTC 3 cut(s) 72, 615, 708
BstBAI YACGTR 1 cut(s) 349
BstC8I GCNNGC 1 cut(s) 183
BstDEI CTNAG 1 cut(s) 115
BstF5I GGATG 4 cut(s) 142, 487, 598, 622
BstFNI CGCG 1 cut(s) 416
BstHHI GCGC 1 cut(s) 511
BstKTI GATC 4 cut(s) 148, 238, 247, 646
BstMAI GTCTC 2 cut(s) 405, 574
BstMBI GATC 4 cut(s) 145, 235, 244, 643
BstMWI GCNNNNNNNGC 1 cut(s) 506
BstNI CCWGG 1 cut(s) 282
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 1 cut(s) 280
BstSLI GKGCMC 2 cut(s) 187, 548
BstSNI TACGTA 1 cut(s) 349
BstUI CGCG 1 cut(s) 416
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
BsuRI GGCC 1 cut(s) 546
BtsCI GGATG 4 cut(s) 142, 487, 598, 622
Cac8I GCNNGC 1 cut(s) 183
CfoI GCGC 1 cut(s) 511
Cfr13I GGNCC 3 cut(s) 4, 544, 545
CseI GACGC 1 cut(s) 422
Csp6I GTAC 2 cut(s) 346, 428
CviAII CATG 2 cut(s) 305, 584
CviJI RGCY 6 cut(s) 29, 164, 546, 656, 757, 811
CviKI_1 RGCY 6 cut(s) 29, 164, 546, 656, 757, 811
CviQI GTAC 2 cut(s) 346, 428
DdeI CTNAG 1 cut(s) 115
DpnI GATC 4 cut(s) 147, 237, 246, 645
DpnII GATC 4 cut(s) 145, 235, 244, 643
Eam1104I CTCTTC 3 cut(s) 72, 615, 708
EarI CTCTTC 3 cut(s) 72, 615, 708
Eco105I TACGTA 1 cut(s) 349
Eco24I GRGCYC 1 cut(s) 548
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 780
EcoO109I RGGNCCY 1 cut(s) 545
EcoRI GAATTC 1 cut(s) 683
EcoRII CCWGG 1 cut(s) 280
EcoT22I ATGCAT 1 cut(s) 360
EcoT38I GRGCYC 1 cut(s) 548
Esp3I CGTCTC 1 cut(s) 405
FaeI CATG 2 cut(s) 308, 587
FalI AAGNNNNNCTT 2 cut(s) 705, 737
FaqI GGGAC 2 cut(s) 263, 581
FatI CATG 2 cut(s) 304, 583
FbaI TGATCA 1 cut(s) 145
FokI GGATG 4 cut(s) 149, 494, 605, 629
FriOI GRGCYC 1 cut(s) 548
FspBI CTAG 2 cut(s) 239, 501
GlaI GCGC 1 cut(s) 510
GsuI CTGGAG 1 cut(s) 303
HaeIII GGCC 1 cut(s) 546
HgaI GACGC 1 cut(s) 422
HhaI GCGC 1 cut(s) 511
Hin1II CATG 2 cut(s) 308, 587
Hin6I GCGC 1 cut(s) 509
HinP1I GCGC 1 cut(s) 509
HinfI GANTC 1 cut(s) 109
Hpy166II GTNNAC 1 cut(s) 170
Hpy188III TCNNGA 5 cut(s) 239, 710, 722, 743, 781
Hpy8I GTNNAC 1 cut(s) 170
Hpy99I CGWCG 1 cut(s) 252
HpyCH4IV ACGT 2 cut(s) 348, 433
HpyCH4V TGCA 4 cut(s) 140, 358, 381, 574
HpyF10VI GCNNNNNNNGC 1 cut(s) 506
HpyF3I CTNAG 1 cut(s) 115
HpySE526I ACGT 2 cut(s) 348, 433
Hsp92II CATG 2 cut(s) 308, 587
HspAI GCGC 1 cut(s) 509
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 4 cut(s) 145, 235, 244, 643
LpnPI CCDG 8 cut(s) 183, 267, 294, 457, 561, 612, 660, 771
LweI GCATC 3 cut(s) 127, 367, 520
MaeI CTAG 2 cut(s) 239, 501
MaeII ACGT 2 cut(s) 348, 433
MaeIII GTNAC 2 cut(s) 110, 785
MalI GATC 4 cut(s) 147, 237, 246, 645
MboI GATC 4 cut(s) 145, 235, 244, 643
MboII GAAGA 6 cut(s) 86, 89, 632, 725, 851, 881
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 2 cut(s) 187, 548
MluCI AATT 7 cut(s) 55, 457, 637, 683, 693, 734, 791
MlyI GAGTC 1 cut(s) 118
MnlI CCTC 9 cut(s) 19, 472, 595, 607, 616, 625, 709, 739, 741
Mph1103I ATGCAT 1 cut(s) 360
MroXI GAANNNNTTC 1 cut(s) 81
MseI TTAA 3 cut(s) 341, 456, 813
MspR9I CCNGG 1 cut(s) 282
MvaI CCWGG 1 cut(s) 282
MvnI CGCG 1 cut(s) 416
MwoI GCNNNNNNNGC 1 cut(s) 506
NdeII GATC 4 cut(s) 145, 235, 244, 643
NlaIII CATG 2 cut(s) 308, 587
NlaIV GGNNCC 2 cut(s) 224, 546
NmuCI GTSAC 2 cut(s) 110, 785
NsiI ATGCAT 1 cut(s) 360
NspI RCATGY 1 cut(s) 587
PciI ACATGT 1 cut(s) 583
PcsI WCGNNNNNNNCGW 1 cut(s) 786
PdmI GAANNNNTTC 1 cut(s) 81
PleI GAGTC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 117
Ppu21I YACGTR 1 cut(s) 349
PscI ACATGT 1 cut(s) 583
PshBI ATTAAT 1 cut(s) 456
PsiI TTATAA 2 cut(s) 441, 537
Psp6I CCWGG 1 cut(s) 280
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 2 cut(s) 224, 546
PspOMI GGGCCC 1 cut(s) 544
PspPI GGNCC 3 cut(s) 4, 544, 545
PsrI GAACNNNNNNTAC 2 cut(s) 477, 509
PsuI RGATCY 1 cut(s) 235
RsaI GTAC 2 cut(s) 347, 429
RsaNI GTAC 2 cut(s) 346, 428
SaqAI TTAA 3 cut(s) 341, 456, 813
Sau3AI GATC 4 cut(s) 145, 235, 244, 643
Sau96I GGNCC 3 cut(s) 4, 544, 545
SchI GAGTC 1 cut(s) 118
ScrFI CCNGG 1 cut(s) 282
SduI GDGCHC 2 cut(s) 187, 548
SetI ASST 7 cut(s) 224, 347, 351, 436, 490, 606, 627
SfaNI GCATC 3 cut(s) 127, 367, 520
SinI GGWCC 1 cut(s) 4
SnaBI TACGTA 1 cut(s) 349
Sse9I AATT 7 cut(s) 55, 457, 637, 683, 693, 734, 791
SspI AATATT 1 cut(s) 331
SspMI CTAG 2 cut(s) 239, 501
StyD4I CCNGG 1 cut(s) 280
TaiI ACGT 2 cut(s) 351, 436
TaqI TCGA 1 cut(s) 780
TaqII GACCGA 1 cut(s) 21
TasI AATT 7 cut(s) 55, 457, 637, 683, 693, 734, 791
TatI WGTACW 1 cut(s) 427
Tru1I TTAA 3 cut(s) 341, 456, 813
Tru9I TTAA 3 cut(s) 341, 456, 813
TseFI GTSAC 2 cut(s) 110, 785
Tsp45I GTSAC 2 cut(s) 110, 785
TspDTI ATGAA 6 cut(s) 498, 633, 696, 705, 811, 882
VpaK11BI GGWCC 1 cut(s) 4
VspI ATTAAT 1 cut(s) 456
XapI RAATTY 3 cut(s) 637, 683, 693
XbaI TCTAGA 1 cut(s) 238
XceI RCATGY 1 cut(s) 587
XcmI CCANNNNNNNNNTGG 1 cut(s) 194
XmnI GAANNNNTTC 1 cut(s) 81
XspI CTAG 2 cut(s) 239, 501
Zsp2I ATGCAT 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.