pycom02g19080

gag-polypeptide of LTR copia-type

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001471
Physical Location & Seq
Forward (+)
17089239 .. 17097376
8138 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 6954 bp
ATGGCACTCCTAACCTCGACTCTCTCCTTATCTCACACCACCAGGCACCAGGGTTTAAGATATCTCCGCCACTTCTTCCAACCCCAACATTTCTTCACTCTCTCTCTCCGCAGCTCCTCTAAAACCCCTTTACCTTTGTTAGAGCTCTGCCATGGCTTGCATTTTCGTCACACTACCAACCCCTTTGGCGTACAAACCCAACACTGCTTCTCTGTCCAACGGCCAGAAGCTACTAGGAGGTGTGTTGAATTTACCTCCTTTCCAAACGCTTTTCTTTCTTTTTATGAAATGTGGTTAATTTTAATGAATTGTGAAATGGGTTTCGCTCAAGTCTGCGAAGGAACAATTGAGAGTCAACGCAATTGCCACGAAATGGGAACCCACCAAGGTTTTTCGGGGCTCTTGCATTATCGGAGTTTTTTTATTTTTGTGGTGGTGATTTGTGGGTGTCAGTTGGTTCCGCAAGCTGACACAGTGCTTATTCTGGCAACTTTATGCGATTATTTTTTATTAGTCCGAATCCAAAATATCTGTGGCAATGGTTATTTAGTTGAGCATTTGAGCTTTTGGACTGTGATAGTTGTGAAGATCCGTAACATTGTCAAATGGCTTAATGTTGATAGGGATTATAATGAAAGACAGAAAGATGTACTAGCTTGGAGGTTAAGAAAATATACTGAAGATTACAGTTTCTATACTTCTCTCTCTCTAAATTCTCTCGACTTTCTCTCTCTATATTTCTGTCTATATTACCAAGTCTTCCGCAGCTCTGATTCTTCGTCTTCATCTCTCTTTCTGCTTCTTGCTGGTTATTTCTGGTTGAATTCTTTGTGTTTACTCTGCAATCTAGGGTTTATCTTCATCCCTAGGGTTCTGCAACTGTACTACTGGTTGCGATTCGTCTTCTTCAATTCGTGCACTCCAAGTCAGTCTTCGACCTGCACGAGTGTAAAAATGGTAACTGCCTCTCAATTACAGCTTCTTCAATCTCCGATAACTGCATTGGTGTCATCGATTTCCACATCTGTGAGTGTTAAACTGGATGATTCGAATTATCTGAATTGGCATTTTCAAATGAAATTACTACTTGAAAGTAATGGAATTCTAGGGTTTGTTGATGGATCTCATCCCTGTCCTGCTCGATTTAGCGCAATATCTGGCGATTCTGTTGATAATTTGTTGTCTTCCACTGATAGTGATGAGTATTTGGTTTGGATGATGCATGATAAAGCCTTGATGCAATTACTTATTGCATCCTTGAGTCCTGTAGCCATGTCTTGTGCGATTGGGAGTACCAGTTCTCGAGAGCTCTGGATTCGATTACAAGAGCAGTTTTCCACTGTCTCGAAGACTAGCATTTTTCAGATGAAATCGAATCTACAAACAATTAAGAAGGGTTCTGATTCCATTACTCAGTATTTGCAGAAGATAAAGGAAGCCAGAGATTATCTTTCTGCTGCTGGGGTTAATTTTCTGGATGAGGACATTGCTATACTTGCACTCAATGGTTTACCCGCAGAGTACAATACTTTTCGGACTGTGATAAGGGGACGTGAGAGTGTCATTTCTCTGAAGGAATTTCGGACACAATTACTGGCTGAAGAAGCTATTCTCGAGTCCACTATCAATGCACCATTTATGACAGCCATGGTTGCCAATACTCCACCTCCGAGAGGATCCTCTCACCCAAACTATGTACCTGGTGGTTTCAAGCCGTTCCCTGTTAATAAGAACAAGGGCAAGGGCAGGTTCCATCACTACACCAGGACTTTTTCACCTCGCCAATATATGCCTACTCAGACACATGTCATTCAAACTCCAAGTCCTGGTATTCTTGGTCAATTTCCCAATACACAGCAGCCTATTCTTTCTGCTCCACCTGAGAAGTCAAGGTGTCAAATTTGTGGCCGATTCAATCACACTACATGGTTCTGTTTTTATAATGAGAAGGGTCCCAACTATATTGGTGCTGCTGCTCATCCTGCCTATTCCCCGATGACGTCCTCTTCACAGCAAGGGTATCAGTACTCTCCAGTTGCTGCCTATCCGTCACAAAGGCCTCACTCACAGGCTCCCCTGCATGCTTATCATACTATGGTATCACAATCAACTCCTTCACCTGCTTCTACCTCTAATTCCCAAATGTGGATCACTGATTCTGGCGCCACGAATCATATGACTGCTGATCTCTCCAACTTATCACTGGCATCTCCTTATCCTACTAATGAAGTCATCCAAACTGCAAATGGTGAAGGTTTGCATGTATCTCACATTGGTAGCACTGTTCTTAACCCTTCTGCTTATCCTATCCACTTGAAATCTGTGTTATATGTTCCCAAATTAACACAGAATCTTCTTTCGGTCCATAGGATATGTTTGGACAATAACTGTTGGCTTATTTTCGATGCCTTCTGTTTTTGGATTCAGGACAAGGCCACAGGGAGGATTCTATACAAAGGGCTGTGCAGTAATGGACTCTATCCCATTTCTTCCCCTGCCACTGTCTGTCCTGCAACTTCTCACAGTTCTGTCCCATCTAAAGCTTTCATTGGTCAGCTTGTTAACTCTAATGTCTGGCACAATCGATTAGGACATCCATCCAATTCTGTTGTATCTCTCATGTTGCATAAGTCTAATATTGTTTTTCCCAAAGACTCTAGTCCTGTCATGTGTCAAAGTTGTTTAGAGGGAAAATTTTGCAAACTCCCTTTTTCTTCCAGTGTCAATAAATCTGTAATTCCATTTGAAGTGGTGCATAGTGATCTTTGGGGTCCTGCACCTTGTAAATCTGTGGATGGGTATCGGTATTATGTTGTATTCATTGATGAATGTACCAGGTATTCCTGGATTTTCCCATTGGTTAATAAATCTGATCTTTGTGCCATTTTTGTTGGGTTTCATGCTTATGTTCTCACCCAATTTTCTGCAAGTATCAAAGTTTTGCAAAGTGATGGGGGTGGTGAATACATTAGCAAATCTCTTCAATCCTTTCTTTTACAAAAAGGCATTTCCCATCAGAAATCATGCCCTTACACCCCTGAACAAAATGGCATGGCTGAACGCAAGCATAGACATCTCATTGAAACCACAGTCACCCTGTTACAAACAGCCAAAATACCCTCCCAGTTTTGGTCTTTTGCATGTCAAACAGCTCTCTATCTTATTAATAGGATGCCTACTCCCATATTGTCTAACAAGTCTCCCTTTGAAGTCCTATATGGTGCTGTCCCAGTCCTTACTCACTTGAGAATTTTTGGGTGTGCTTGTTATCCTTTATTACGCCCCTATAACTCCACTAAACTTCAAGCCAAGACTACCAAGTGTGTCTTCTTGGGTTATGCTTCCAAATACAAAGGGTACATTTGTTATCTTGTGTCTCAACATAAATTCTTTGTGTCTAGACATGTGATGTTTAATGAGTATGAATTTCCTTATACAACTCTTTTGGCACAAACACAAACCCAATCCTCTGCCTCATTTGATGGTCTTTTGTCATCTCATGTGGTCCCATCTCAGAATATAGTCACATCTCCTTCTCCACTTATTAATTCCAGCCCTTCAAGTCCCTCTGAACCATCCCCCAGTCCACCATCTGTTGTGTCTTCCTCCATACCTCAATCCATTACTGGTGCAGTTTCAGCCACATCTCCCATCCCTGTGGCTCCTGATCATAGTCCAGATATTGTACACGTGGTTTCCTCTATCCCGCAGTTCAACTTACATCCGATGCAAACTCGAAGTAAGAGTGGTATTATAAAGAAGAAGGTGTTCCTTTCCACTCTTGAGACCTCTAATGATATTGATCTGTCCCTAAGTGAACCAGCCTCATATAAATCTGCTCTTAAGAGTTCCGTGTGGTCTGAAGCAATGAAGGATGAGATCAGTGCTCTACATAAGCAAGGGACATGGAGTTTAGTCTCTTTACCAGCCAATAAAAACTTAGTGGGCTGTAAATGGATCTTCAAAATCAAAAGGCATTCAGATGGGTCCATAGCAAGGCACAAAGCTCGCTTAGTTGCCAAAGGCTTTAGTCAAGAACCGGGTCTTGATTACAGTGAAACGTTTAGCCCAGTTGTCAAACCCACCACAGTGAGGATTGTTTTAGCTCTTGCTGCACATTTCAATTGGTCACTCCATCAACTCGATGTCAAGAATGCCTTTCTTCATGGCATTCTGCAAGAAGAAGTATACATGGCCCAGCCACCTGGATTTGAGGATCCTCAGAATCTGTCTTTGGTCTGCAAGTTACATAAATCTCTTTATGGCTTGAAGCAGGCACCCAGGGCATGGAATGAAAGGTTCACTTCTTTCTTGCCTCGGTTGGGATTTCATAACACTTACTCTGATTCCTCCTTATTTGTGAAGACTGTTGGTGCCCATACTGTGATATTGTTGCTCTATGTTGATGACATTATCCTCACAGGCAGTGATCTTCCTGAGCTCCAACAAGTGATTCGGGATTTAACTTCTGAATTTGATATCAAGGACTTAGGTCCCCTTCACTTCTTTCTGGGCATTCAGATTTCTAAAACCCCAAACGGTCTGTTTCTGTCACAACAGAAATATGTGCAGGATTTGCTTGCTAAAACAGACATGCTTGAGTCCAAGCCGTGTGATACACCCTGTCTTCCATACACCAGATTACTCACGGATGATGGGGTACCATATGCTAATCCTACCATGTATAGAAGTGTAGTGGGCGCACTTCAATACCTTACATTTACCAGGCCGGATATTGCCTTTTCTGTGCACCAAGTTTGCCAGTTCATGCAGAATCCGATGGTATCTCATTTCACTACTGTCAAAAGGATTTTGCGCTATTTGAAAGGCACGATGGAGTTTGGTATCAACTACTCTAAGGGTGATTTGACTTTACATGCGTTTAGCGATGCTGATTGGGCGGGGGATCCCAATGATCGCCGGTCAACAACTGGTCTGGCTGTGTTCTTAGGCAATAACCCTATCTCCTGGTCATCCAAGAAGCAACAAACAGTTTCACGATCCTCCACTGAGGCGGAATATCGTGCTTTATCTACCACGGCAGCTGAACTGGACTGGATTCAACAGATTCTTACTTTCCTGCATATTCCCATCTCTGCGCCACCTGTGCTGTTTTGTGACAACCTTTCTGCTATTGCCTTGTCTTTCAACCCAGTTCAACATCAACGGACCAAACACATTGAAATTGATGTGCACTTTGTGCGGGAGAGGGTTGCCAAACACAAGCTACTGGTGCAGTTTGTATCCTCCAAAGAACAGTTTGCTGATGTTTTAACCAAAGGCCTCAGTGCCCCTTTGTTTCGCTTTCATTGTACCAATCTCATGTTAGTATCCTCCAAACATGATGTTGACACTTGTATGGAGATTAGAGAGTTAGTTAAGTTGTTAGTTTCTTGGAGGTTAAGAAAATATACTGAAGATTACAGTTTCTATACTTCTCTCTCTCTAAATTCTCTCGACTTTCTCTCTCTATATTTCTTCCTACGACAGTTTCTGACCATTTGGATTTTTTTTTTCTTCTTTCAAATATATATAAATGAATCATTTCCTTTTAAACATAAATATGTATGTTTTGTTAGTTCATTTCATCTTTATGTGGTGAAGATCGACCCTAAAAATCATAAAAAGATGATTATATTTGTATATCATCTCTACGGTATCCTCCCACTTATCATATCGTTCCCATGTTATCATGGTTGTTGTATGTTATCCTTTTGTATGTTCTATGTAGAAATTTTCTATGAACTTAAGCCATTGTATGAGGGCCTGGGAAATGGGATGAGTGCCATTGGAGGAAACTTGACAGTGGAATGGTACCTGGTCAGAGGGGTTTTTATAATTCATTGGGAATACAGTGCGATAGCAGTAGATATAATTCTTCTTTGCGGTCTCGTAGTTCTTCCTTCTAACTTTAATCAAAAGATTTTTCAGCCCTGTCTTAATTTTATATTCTGGCAGGATTTGTGTCGGATTTCTTTTTTACGTGTGACTCCTCGATGTTCAAATTCAAGTAGTTTGATAATAGGTGGTTACAAAGTGCAAACTATACTAACAATCTTGAAATCGATCAGGTTAAAAAGCCAAGTTCGCATTAGAGGCATTGTAATTAGGTATAACCTTTGGAGTTTAAGGGTTGGTTGTAAGGTTTATGTATTGCCTGATTTTTTATCCTTCACTTTTTGGTTAATTAGCATTTATGCTTTGAAAGCAGTTGGTTTTAAAACGGACTTCATTGTTTACTTTTCCTTTTTTGTTATTATATCAAATGGAAAAATATGTAAAACGCTATGCCACAGAAATCAGCTGGAGGAGTTTTGCTGCCCAAGTCAGCTGTTAAATTTGAACGCTATCTTATGGGAGAAGTGTGCCTACTCTTTCTTTTTTGTATGGATAATTGAACAAGCATTGTTATTCTCAGATTCTTTCTGTTGGTTAAAACGTTCCCAAATAATGATCACACTTTTCTGGTGTGAATTAGTTTGTCTATTGTTTAAAACTTTTTTAAACTTTTATCCCGAAGTAGTTCTTTTTTCGGACATAAATGCATATGAGGTAAATAGAATTAAGTTCATAAACATTAAGGAATGGACTTCCTCCATTTTGTATGAGTTATTCTGCTTATCTTTTGGTTTTCCTGTGACTTTATTCCATGACACTGTTAACCTAATCCTGGCTGTCCTAGCATTATTAGTGGTTTTCAATTTCCCGCTCCCCCTCCACACCTATGTCGCCGGGCCACCTTTCTTTCATTATTCAATCCAGAGGGTCTGTGATACAGTCACAACCTATCTTCTGACACAAAATCGCCACACCTTGCTGTGTACGCCAATAACGATCGACTCTAAAATATGGAACGGAGTAGAGACAGAATATACTCCATGTAAACTGAAGTCTGTTGTGGAAATGTTCTACTGTGATCCATGTAATGGCTGCATATTGATGCTTACACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 gag_pre-integrs

Protein Analysis

2318

Amino Acids

262.59

Weight (kDa)

8.75

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 359 - 536 8.8e-19 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 716 - 791 4.6e-14 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 825 - 898 1.5e-08 GAG-pre-integrase domain
rve PF00665 914 - 1013 3.1e-10 Integrase core domain
SH3_retrovirus PF25597 1087 - 1147 4.7e-20 Retroviral polymerase SH3-like domain
RVT_2 PF07727 1302 - 1543 3.7e-76 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 630, 1941, 3755, 5837
AarI CACCTGC 1 cut(s) 2128
AatII GACGTC 1 cut(s) 2003
Acc36I ACCTGC 3 cut(s) 947, 1737, 2128
Acc65I GGTACC 2 cut(s) 4660, 5814
AccB1I GGYRCC 6 cut(s) 45, 2162, 4276, 4373, 4660, 5814
AccB7I CCANNNNNTGG 4 cut(s) 373, 1826, 4287, 6926
AccBSI CCGCTC 1 cut(s) 6709
AccI GTMKAC 1 cut(s) 4188
AclI AACGTT 2 cut(s) 4061, 6439
AcoI YGGCCR 2 cut(s) 221, 1906
AcuI CTGAAG 6 cut(s) 699, 1592, 1620, 3882, 5436, 6908
AcvI CACGTG 1 cut(s) 3691
AcyI GRCGYC 2 cut(s) 2000, 2163
AdeI CACNNNGTG 1 cut(s) 5200
AflII CTTAAG 2 cut(s) 3842, 5747
AflIII ACRYGT 4 cut(s) 1804, 3403, 3688, 5983
AjiI CACGTC 1 cut(s) 1553
AjuI GAANNNNNNNTTGG 8 cut(s) 916, 948, 3311, 3343, 4217, 4249, 4437, 4469
AleI CACNNNNGTG 2 cut(s) 1025, 4088
AloI GAACNNNNNNTCC 2 cut(s) 1282, 1314
Alw21I GWGCWC 7 cut(s) 147, 920, 1311, 3889, 4443, 4752, 5196
Alw26I GTCTC 7 cut(s) 1348, 3204, 3381, 3779, 3922, 5894, 6857
Alw44I GTGCAC 3 cut(s) 916, 4748, 5192
AlwNI CAGNNNCTG 3 cut(s) 2039, 4228, 5494
Ama87I CYCGRG 2 cut(s) 1302, 1613
AoxI GGCC 9 cut(s) 221, 1906, 2057, 2433, 4194, 4727, 5281, 5764, 6734
ApaLI GTGCAC 3 cut(s) 916, 4748, 5192
ArsI GACNNNNNNTTYG 2 cut(s) 1807, 1839
AseI ATTAAT 2 cut(s) 3165, 3546
Asp700I GAANNNNTTC 4 cut(s) 1608, 3767, 5544, 6522
Asp718I GGTACC 2 cut(s) 4660, 5814
AspA2I CCTAGG 1 cut(s) 866
AspLEI GCGC 5 cut(s) 1151, 2165, 4703, 4818, 5101
AsuC2I CCSGG 2 cut(s) 4041, 6733
AsuII TTCGAA 1 cut(s) 1049
AvaI CYCGRG 2 cut(s) 1302, 1613
AvaII GGWCC 7 cut(s) 1952, 2362, 2771, 3505, 3987, 4493, 5169
AvrII CCTAGG 1 cut(s) 866
BaeGI GKGCMC 5 cut(s) 920, 4378, 4752, 5196, 5293
BaeI ACNNNNGTAYC 4 cut(s) 2082, 2115, 3349, 3382
BamHI GGATCC 3 cut(s) 1676, 4216, 4906
BanI GGYRCC 6 cut(s) 45, 2162, 4276, 4373, 4660, 5814
BanII GRGCYC 4 cut(s) 147, 402, 1311, 4443
BarI GAAGNNNNNNTAC 2 cut(s) 6353, 6385
BauI CACGAG 1 cut(s) 943
BbrPI CACGTG 1 cut(s) 3691
Bbv12I GWGCWC 7 cut(s) 147, 920, 1311, 3889, 4443, 4752, 5196
BceAI ACGGC 4 cut(s) 236, 1699, 4594, 5055
BcgI CGANNNNNNTGC 2 cut(s) 3989, 4023
BciVI GTATCC 3 cut(s) 5254, 5341, 5669
BclI TGATCA 2 cut(s) 3667, 6453
BcnI CCSGG 2 cut(s) 4041, 6733
BcoDI GTCTC 7 cut(s) 1348, 3204, 3381, 3779, 3922, 5894, 6857
BfaI CTAG 9 cut(s) 234, 653, 848, 867, 1106, 1353, 2658, 3399, 6680
BfmI CTRYAG 1 cut(s) 1266
BfoI RGCGCY 1 cut(s) 2166
BfrI CTTAAG 2 cut(s) 3842, 5747
BfuAI ACCTGC 3 cut(s) 947, 1737, 2128
BfuI GTATCC 3 cut(s) 5254, 5341, 5669
BlnI CCTAGG 1 cut(s) 866
BmcAI AGTACT 1 cut(s) 2027
Bme18I GGWCC 7 cut(s) 1952, 2362, 2771, 3505, 3987, 4493, 5169
BmeT110I CYCGRG 2 cut(s) 1302, 1613
BmgBI CACGTC 1 cut(s) 1553
BmrI ACTGGG 5 cut(s) 3118, 3224, 3576, 4064, 5147
BmsI GCATC 9 cut(s) 1209, 1227, 1262, 2216, 2395, 3162, 3717, 4879, 6930
BmuI ACTGGG 5 cut(s) 3118, 3224, 3576, 4064, 5147
BoxI GACNNNNGTC 3 cut(s) 1805, 2658, 4491
BpmI CTGGAG 2 cut(s) 2016, 6326
Bpu10I CCTNAGC 1 cut(s) 4437
Bpu14I TTCGAA 1 cut(s) 1049
BpuEI CTTGAG 5 cut(s) 312, 1279, 3265, 3803, 4619
BpuMI CCSGG 2 cut(s) 4041, 6733
Bsa29I ATCGAT 3 cut(s) 1013, 2584, 6065
BsaAI YACGTR 2 cut(s) 3691, 5984
BsaBI GATNNNNATC 2 cut(s) 2799, 3801
BsaHI GRCGYC 2 cut(s) 2000, 2163
BsaI GGTCTC 2 cut(s) 3779, 5894
BsaXI ACNNNNNCTCC 6 cut(s) 1282, 1312, 1651, 1681, 4949, 4979
Bse118I RCCGGY 1 cut(s) 4920
Bse3DI GCAATG 3 cut(s) 544, 1485, 3873
Bse8I GATNNNNATC 2 cut(s) 2799, 3801
BseCI ATCGAT 3 cut(s) 1013, 2584, 6065
BseJI GATNNNNATC 2 cut(s) 2799, 3801
BseMI GCAATG 3 cut(s) 544, 1485, 3873
BseMII CTCAG 9 cut(s) 1427, 1811, 1872, 3527, 4235, 4428, 5001, 5299, 6429
BseRI GAGGAG 3 cut(s) 106, 5982, 6323
BseSI GKGCMC 5 cut(s) 920, 4378, 4752, 5196, 5293
BseYI CCCAGC 2 cut(s) 1460, 4197
BsgI GTGCAG 7 cut(s) 925, 2485, 2760, 3651, 4098, 4589, 5255
Bsh1285I CGRYCG 1 cut(s) 6837
BshFI GGCC 9 cut(s) 223, 1908, 2059, 2435, 4196, 4729, 5283, 5766, 6736
BshNI GGYRCC 6 cut(s) 45, 2162, 4276, 4373, 4660, 5814
BshVI ATCGAT 3 cut(s) 1013, 2584, 6065
BsiEI CGRYCG 1 cut(s) 6837
BsiHKAI GWGCWC 7 cut(s) 147, 920, 1311, 3889, 4443, 4752, 5196
BsiHKCI CYCGRG 2 cut(s) 1302, 1613
BsiSI CCGG 4 cut(s) 4040, 4730, 4921, 6732
BslFI GGGAC 9 cut(s) 1563, 1938, 2516, 3212, 3491, 3549, 3793, 3916, 4479
BsmAI GTCTC 7 cut(s) 1348, 3204, 3381, 3779, 3922, 5894, 6857
BsmFI GGGAC 9 cut(s) 1563, 1938, 2516, 3212, 3491, 3549, 3793, 3916, 4479
BsmI GAATGC 4 cut(s) 3976, 4159, 4170, 4515
BsnI GGCC 9 cut(s) 223, 1908, 2059, 2435, 4196, 4729, 5283, 5766, 6736
Bso31I GGTCTC 2 cut(s) 3779, 5894
BsoBI CYCGRG 2 cut(s) 1302, 1613
Bsp119I TTCGAA 1 cut(s) 1049
Bsp1407I TGTACA 1 cut(s) 3685
Bsp19I CCATGG 2 cut(s) 151, 1647
BspANI GGCC 9 cut(s) 223, 1908, 2059, 2435, 4196, 4729, 5283, 5766, 6736
BspCNI CTCAG 9 cut(s) 1426, 1810, 1873, 3526, 4234, 4429, 5002, 5298, 6428
BspDI ATCGAT 3 cut(s) 1013, 2584, 6065
BspMI ACCTGC 3 cut(s) 947, 1737, 2128
BspT104I TTCGAA 1 cut(s) 1049
BspT107I GGYRCC 6 cut(s) 45, 2162, 4276, 4373, 4660, 5814
BspTI CTTAAG 2 cut(s) 3842, 5747
BspTNI GGTCTC 2 cut(s) 3779, 5894
BsrBI CCGCTC 1 cut(s) 6709
BsrDI GCAATG 3 cut(s) 544, 1485, 3873
BsrFI RCCGGY 1 cut(s) 4920
BsrGI TGTACA 1 cut(s) 3685
BssAI RCCGGY 1 cut(s) 4920
BssNAI GTATAC 1 cut(s) 4189
BssNI GRCGYC 2 cut(s) 2000, 2163
BssSI CACGAG 1 cut(s) 943
BssT1I CCWWGG 4 cut(s) 151, 385, 866, 1647
Bst1107I GTATAC 1 cut(s) 4189
Bst2BI CACGAG 1 cut(s) 943
Bst6I CTCTTC 2 cut(s) 2011, 2985
BstACI GRCGYC 2 cut(s) 2000, 2163
BstAFI CTTAAG 2 cut(s) 3842, 5747
BstAPI GCANNNNNTGC 2 cut(s) 4576, 5200
BstAUI TGTACA 1 cut(s) 3685
BstBAI YACGTR 2 cut(s) 3691, 5984
BstBI TTCGAA 1 cut(s) 1049
BstC8I GCNNGC 7 cut(s) 158, 465, 2082, 3065, 4009, 4275, 4581
BstDSI CCRYGG 3 cut(s) 151, 1647, 5037
BstENI CCTNNNNNAGG 1 cut(s) 1671
BstH2I RGCGCY 1 cut(s) 2166
BstHHI GCGC 5 cut(s) 1151, 2165, 4703, 4818, 5101
BstMAI GTCTC 7 cut(s) 1348, 3204, 3381, 3779, 3922, 5894, 6857
BstMCI CGRYCG 1 cut(s) 6837
BstNSI RCATGY 7 cut(s) 1808, 2084, 2264, 3144, 3407, 4597, 4880
BstPAI GACNNNNGTC 3 cut(s) 1805, 2658, 4491
BstSFI CTRYAG 1 cut(s) 1266
BstSLI GKGCMC 5 cut(s) 920, 4378, 4752, 5196, 5293
BstX2I RGATCY 6 cut(s) 588, 1121, 1676, 3957, 4216, 4906
BstXI CCANNNNNNTGG 2 cut(s) 3658, 4205
BstYI RGATCY 6 cut(s) 588, 1121, 1676, 3957, 4216, 4906
BstZ17I GTATAC 1 cut(s) 4189
Bsu15I ATCGAT 3 cut(s) 1013, 2584, 6065
BsuI GTATCC 3 cut(s) 5254, 5341, 5669
BsuRI GGCC 9 cut(s) 223, 1908, 2059, 2435, 4196, 4729, 5283, 5766, 6736
BsuTUI ATCGAT 3 cut(s) 1013, 2584, 6065
BtgI CCRYGG 3 cut(s) 151, 1647, 5037
BtgZI GCGATG 1 cut(s) 4902
BtrI CACGTC 1 cut(s) 1553
BtsI GCAGTG 2 cut(s) 202, 4432
BveI ACCTGC 3 cut(s) 947, 1737, 2128
Cac8I GCNNGC 7 cut(s) 158, 465, 2082, 3065, 4009, 4275, 4581
CaiI CAGNNNCTG 3 cut(s) 2039, 4228, 5494
CfoI GCGC 5 cut(s) 1151, 2165, 4703, 4818, 5101
Cfr10I RCCGGY 1 cut(s) 4920
ClaI ATCGAT 3 cut(s) 1013, 2584, 6065
CsiI ACCWGGT 3 cut(s) 1699, 2834, 5817
DinI GGCGCC 1 cut(s) 2164
DraI TTTAAA 4 cut(s) 5554, 6220, 6493, 6504
DraIII CACNNNGTG 1 cut(s) 5200
EaeI YGGCCR 2 cut(s) 221, 1906
Eam1104I CTCTTC 2 cut(s) 2011, 2985
EarI CTCTTC 2 cut(s) 2011, 2985
EciI GGCGGA 2 cut(s) 56, 5030
Ecl136II GAGCTC 3 cut(s) 145, 1309, 4441
Eco130I CCWWGG 4 cut(s) 151, 385, 866, 1647
Eco147I AGGCCT 2 cut(s) 2059, 5283
Eco24I GRGCYC 4 cut(s) 147, 402, 1311, 4443
Eco31I GGTCTC 2 cut(s) 3779, 5894
Eco32I GATATC 2 cut(s) 62, 4480
Eco47I GGWCC 7 cut(s) 1952, 2362, 2771, 3505, 3987, 4493, 5169
Eco53kI GAGCTC 3 cut(s) 145, 1309, 4441
Eco57I CTGAAG 6 cut(s) 699, 1592, 1620, 3882, 5436, 6908
Eco72I CACGTG 1 cut(s) 3691
Eco88I CYCGRG 2 cut(s) 1302, 1613
EcoICRI GAGCTC 3 cut(s) 145, 1309, 4441
EcoNI CCTNNNNNAGG 1 cut(s) 1671
EcoO109I RGGNCCY 4 cut(s) 1952, 2771, 4493, 5764
EcoRI GAATTC 2 cut(s) 823, 1101
EcoRV GATATC 2 cut(s) 62, 4480
EcoT14I CCWWGG 4 cut(s) 151, 385, 866, 1647
EcoT22I ATGCAT 2 cut(s) 1224, 6547
EcoT38I GRGCYC 4 cut(s) 147, 402, 1311, 4443
EgeI GGCGCC 1 cut(s) 2164
EheI GGCGCC 1 cut(s) 2164
ErhI CCWWGG 4 cut(s) 151, 385, 866, 1647
FaqI GGGAC 9 cut(s) 1563, 1938, 2516, 3212, 3491, 3549, 3793, 3916, 4479
FauI CCCGC 5 cut(s) 1522, 3714, 4894, 5196, 6714
FauNDI CATATG 3 cut(s) 2175, 4666, 6547
FbaI TGATCA 2 cut(s) 3667, 6453
FblI GTMKAC 1 cut(s) 4188
FriOI GRGCYC 4 cut(s) 147, 402, 1311, 4443
FspBI CTAG 9 cut(s) 234, 653, 848, 867, 1106, 1353, 2658, 3399, 6680
GlaI GCGC 5 cut(s) 1150, 2164, 4702, 4817, 5100
GsaI CCCAGC 2 cut(s) 1464, 4201
GsuI CTGGAG 2 cut(s) 2016, 6326
HaeII RGCGCY 1 cut(s) 2166
HaeIII GGCC 9 cut(s) 223, 1908, 2059, 2435, 4196, 4729, 5283, 5766, 6736
HapII CCGG 4 cut(s) 4040, 4730, 4921, 6732
HhaI GCGC 5 cut(s) 1151, 2165, 4703, 4818, 5101
Hin1I GRCGYC 2 cut(s) 2000, 2163
Hin6I GCGC 5 cut(s) 1149, 2163, 4701, 4816, 5099
HinP1I GCGC 5 cut(s) 1149, 2163, 4701, 4816, 5099
HincII GTYRAC 5 cut(s) 356, 2563, 4926, 5350, 6661
HindII GTYRAC 5 cut(s) 356, 2563, 4926, 5350, 6661
HindIII AAGCTT 1 cut(s) 2541
HpaI GTTAAC 2 cut(s) 2563, 6661
HpaII CCGG 4 cut(s) 4040, 4730, 4921, 6732
HpyCH4IV ACGT 6 cut(s) 1552, 2000, 3690, 4061, 5983, 6439
HpySE526I ACGT 6 cut(s) 1552, 2000, 3690, 4061, 5983, 6439
Hsp92I GRCGYC 2 cut(s) 2000, 2163
HspAI GCGC 5 cut(s) 1149, 2163, 4701, 4816, 5099
KasI GGCGCC 1 cut(s) 2162
KflI GGGWCCC 1 cut(s) 1952
KpnI GGTACC 2 cut(s) 4664, 5818
Ksp22I TGATCA 2 cut(s) 3667, 6453
KspAI GTTAAC 2 cut(s) 2563, 6661
LmnI GCTCC 6 cut(s) 119, 1879, 2077, 3667, 4446, 6714
LweI GCATC 9 cut(s) 1209, 1227, 1262, 2216, 2395, 3162, 3717, 4879, 6930
MabI ACCWGGT 3 cut(s) 1699, 2834, 5817
MaeI CTAG 9 cut(s) 234, 653, 848, 867, 1106, 1353, 2658, 3399, 6680
MaeII ACGT 6 cut(s) 1552, 2000, 3690, 4061, 5983, 6439
MbiI CCGCTC 1 cut(s) 6709
MfeI CAATTG 3 cut(s) 345, 361, 4123
MflI RGATCY 6 cut(s) 588, 1121, 1676, 3957, 4216, 4906
Mly113I GGCGCC 1 cut(s) 2163
MlyI GAGTC 9 cut(s) 13, 361, 1270, 1625, 2469, 2648, 4610, 5983, 6833
MmeI TCCRAC 5 cut(s) 103, 241, 2217, 4468, 5948
Mph1103I ATGCAT 2 cut(s) 1224, 6547
MroXI GAANNNNTTC 4 cut(s) 1608, 3767, 5544, 6522
MspA1I CMGCKG 3 cut(s) 5045, 6304, 6331
MspCI CTTAAG 2 cut(s) 3842, 5747
MspI CCGG 4 cut(s) 4040, 4730, 4921, 6732
MunI CAATTG 3 cut(s) 345, 361, 4123
Mva1269I GAATGC 4 cut(s) 3976, 4159, 4170, 4515
NarI GGCGCC 1 cut(s) 2163
NciI CCSGG 2 cut(s) 4041, 6733
NcoI CCATGG 2 cut(s) 151, 1647
NdeI CATATG 3 cut(s) 2175, 4666, 6547
NsiI ATGCAT 2 cut(s) 1224, 6547
NspI RCATGY 7 cut(s) 1808, 2084, 2264, 3144, 3407, 4597, 4880
NspV TTCGAA 1 cut(s) 1049
OliI CACNNNNGTG 2 cut(s) 1025, 4088
PaeI GCATGC 1 cut(s) 2084
PaeR7I CTCGAG 2 cut(s) 1302, 1613
PaqCI CACCTGC 1 cut(s) 2128
PasI CCCWGGG 1 cut(s) 4281
PceI AGGCCT 2 cut(s) 2059, 5283
PciI ACATGT 2 cut(s) 1804, 3403
PcsI WCGNNNNNNNCGW 2 cut(s) 941, 6830
PctI GAATGC 4 cut(s) 3976, 4159, 4170, 4515
PdmI GAANNNNTTC 4 cut(s) 1608, 3767, 5544, 6522
PflMI CCANNNNNTGG 4 cut(s) 373, 1826, 4287, 6926
PfoI TCCNGGA 1 cut(s) 2843
Ple19I CGATCG 1 cut(s) 6837
PleI GAGTC 9 cut(s) 13, 360, 1269, 1624, 2469, 2648, 4609, 5983, 6833
PluTI GGCGCC 1 cut(s) 2166
PmaCI CACGTG 1 cut(s) 3691
PmlI CACGTG 1 cut(s) 3691
PpsI GAGTC 9 cut(s) 13, 360, 1269, 1624, 2469, 2648, 4609, 5983, 6833
Ppu21I YACGTR 2 cut(s) 3691, 5984
PpuMI RGGWCCY 3 cut(s) 1952, 2771, 4493
PscI ACATGT 2 cut(s) 1804, 3403
PshAI GACNNNNGTC 3 cut(s) 1805, 2658, 4491
PshBI ATTAAT 2 cut(s) 3165, 3546
PsiI TTATAA 4 cut(s) 630, 1941, 3755, 5837
Psp124BI GAGCTC 3 cut(s) 147, 1311, 4443
Psp1406I AACGTT 2 cut(s) 4061, 6439
Psp5II RGGWCCY 3 cut(s) 1952, 2771, 4493
PspCI CACGTG 1 cut(s) 3691
PspFI CCCAGC 2 cut(s) 1460, 4197
PspPPI RGGWCCY 3 cut(s) 1952, 2771, 4493
PstNI CAGNNNCTG 3 cut(s) 2039, 4228, 5494
PsuI RGATCY 6 cut(s) 588, 1121, 1676, 3957, 4216, 4906
PvuI CGATCG 1 cut(s) 6837
PvuII CAGCTG 3 cut(s) 5045, 6304, 6331
SacI GAGCTC 3 cut(s) 147, 1311, 4443
ScaI AGTACT 1 cut(s) 2027
SchI GAGTC 9 cut(s) 13, 361, 1270, 1625, 2469, 2648, 4610, 5983, 6833
SexAI ACCWGGT 3 cut(s) 1699, 2834, 5817
SfaNI GCATC 9 cut(s) 1209, 1227, 1262, 2216, 2395, 3162, 3717, 4879, 6930
SfcI CTRYAG 1 cut(s) 1266
SfoI GGCGCC 1 cut(s) 2164
Sfr274I CTCGAG 2 cut(s) 1302, 1613
SfuI TTCGAA 1 cut(s) 1049
SinI GGWCC 7 cut(s) 1952, 2362, 2771, 3505, 3987, 4493, 5169
SlaI CTCGAG 2 cut(s) 1302, 1613
SmlI CTYRAG 9 cut(s) 327, 1258, 1302, 1613, 3244, 3782, 3842, 4598, 5747
SmoI CTYRAG 9 cut(s) 327, 1258, 1302, 1613, 3244, 3782, 3842, 4598, 5747
SphI GCATGC 1 cut(s) 2084
SseBI AGGCCT 2 cut(s) 2059, 5283
SspDI GGCGCC 1 cut(s) 2162
SspI AATATT 1 cut(s) 2638
SspMI CTAG 9 cut(s) 234, 653, 848, 867, 1106, 1353, 2658, 3399, 6680
SstI GAGCTC 3 cut(s) 147, 1311, 4443
StuI AGGCCT 2 cut(s) 2059, 5283
StyI CCWWGG 4 cut(s) 151, 385, 866, 1647
TaiI ACGT 6 cut(s) 1555, 2003, 3693, 4064, 5986, 6442
TaqII GACCGA 1 cut(s) 2350
TatI WGTACW 5 cut(s) 649, 882, 1521, 2025, 3685
TspGWI ACGGA 7 cut(s) 581, 2037, 3841, 4664, 5182, 6239, 6870
Van91I CCANNNNNTGG 4 cut(s) 373, 1826, 4287, 6926
Vha464I CTTAAG 2 cut(s) 3842, 5747
VneI GTGCAC 3 cut(s) 916, 4748, 5192
VpaK11BI GGWCC 7 cut(s) 1952, 2362, 2771, 3505, 3987, 4493, 5169
VspI ATTAAT 2 cut(s) 3165, 3546
XagI CCTNNNNNAGG 1 cut(s) 1671
XbaI TCTAGA 1 cut(s) 3398
XceI RCATGY 7 cut(s) 1808, 2084, 2264, 3144, 3407, 4597, 4880
XcmI CCANNNNNNNNNTGG 3 cut(s) 530, 2200, 2243
XhoI CTCGAG 2 cut(s) 1302, 1613
XmaJI CCTAGG 1 cut(s) 866
XmiI GTMKAC 1 cut(s) 4188
XmnI GAANNNNTTC 4 cut(s) 1608, 3767, 5544, 6522
XspI CTAG 9 cut(s) 234, 653, 848, 867, 1106, 1353, 2658, 3399, 6680
ZraI GACGTC 1 cut(s) 2001
ZrmI AGTACT 1 cut(s) 2027
Zsp2I ATGCAT 2 cut(s) 1224, 6547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.