pycom03g18890

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001544
Physical Location & Seq
Forward (+)
19717547 .. 19720056
2510 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1461 bp
ATGAAGAACTTGGCGGCAACTCTTCACATCTCACAAATGGCTTCCTTCTCCACCTTGAACCTTTCACCAAGTCCAAAACCCACAAAACCATTACCCCATTCTCTTCTAAAACCACTGATTCCGGCACCCGAAATCGATACCCGAACTCAAATCCAAACCCCTTACCCGAAGCTCATACTCACCGGCCCTTCCCAGCTCTCCGGCCATGTACCCATCAGCGGCTCCAAGAACTCAGCTTTGCCGATTCTGGCGGCGACCCTTTGCTGCTCAGGCACTTCAAGGATTCAAAACGTGCCCGATTTGTTCGATACTCGCACAATGGCTTCGGTTTTAGTGTCTCTTGGAGCTAAAGTTGAAGTCTTGGACAACGAAATGTTGGTTAACACCAATGGGGTTGGCTCAGTTGAGCCTAATGCGGATGAAATTCAAAAGATCAGAGGTGGGTTTTTCGTCATTGGGCCGTTGGTTGCCCGATTCGGGGAGGCGGTGGTTGCATTACCCGGTGGCTGCAATATTGGGGCTCGACCAGTGGACCTATACATTCGGGGTCTTCAATGTCTTGGCGCTGTTGTTGAATTGAGGGATGGGAAAGTTCAGGCATATGCCGCAAATGGAAGAGGATTAGTTGGAGGAAGTTTCCAACTTGATTACCCGAGTGTGGGGGCAACGGAAACTCTTATGATGGCTGCATGTATGGCTGATGGAAAAACTGTACTGTCCAATGTTGCCAGAGAACCAGAGGTGGTTGATCTTGCTAGGTTTCTTACCAACTGTGGGGCCTGTGTGGATGGAGCAGGCAGCAACAAGCTTGTTATCAAGGGAAAATCCCAGTTGCATGGTTGTGAATGCGTTGTTGCACCTGATCGTATTGAAGCAGGTACATTTATGCTTGCTGCAGCTATTACTCGCTCATGCATCTCGATATCACCTGTCATACCTTGCCAAATGTCCTCTTTGATACACAAACTATCGGCCGTTGGTTGCAAAATCAAGAAATGTTCTCATGATACCTTGGAAGTTTCAGCAGTGTCCGGACATGGGGGTGCAAATTTGCGAAGCGTTGTGGTTAAGACAGGCCCATATCCTGGGTTTCCCACAGACCTCCAACCACAAATAATGGCTTTTCTGACCACGTGCAATGGTTTAAGTTCTGTAGAGGAATCTGTTTTTGACAAACGCATGAGTCATGTAAGCGAACTGCTAAAGCTTGGAGCAAAGATTCAGGTCTGTGCGAGCACTGCTCTGGTTTATGGGAAAGACAATGGAAGTGTCTTGAATGGTTCCTCGCTTGTTGCAAATGACATTAGGGGTGGGATGTCACTGGTATTAGCTGGGTTGGCCGCAGAAGGCACTACAGAGATCAGCGGTGTTGCTCATATTGACCGTGGTTATGAGAATTTAGATATGAAACTTCGTTTTCTGGGATCTGATGTCAAAAGATTAATGCCTCTTGCATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

487

Amino Acids

50.82

Weight (kDa)

8.3

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 61 - 468 1.9e-91 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 866
AccB1I GGYRCC 1 cut(s) 124
AccB7I CCANNNNNTGG 1 cut(s) 1085
AccIII TCCGGA 1 cut(s) 1031
AciI CCGC 8 cut(s) 14, 219, 251, 416, 485, 606, 1341, 1365
AclWI GGATC 1 cut(s) 1432
AcoI YGGCCR 3 cut(s) 202, 972, 1338
AcsI RAATTY 3 cut(s) 423, 1048, 1396
AcvI CACGTG 1 cut(s) 1134
AfaI GTAC 3 cut(s) 210, 714, 880
AfiI CCNNNNNNNGG 8 cut(s) 218, 477, 478, 658, 659, 774, 1038, 1085
AgsI TTSAA 9 cut(s) 58, 279, 287, 356, 428, 554, 575, 872, 1276
AjnI CCWGG 1 cut(s) 1084
AluBI AGCT 8 cut(s) 172, 196, 236, 347, 808, 899, 1207, 1331
AluI AGCT 8 cut(s) 172, 196, 236, 347, 808, 899, 1207, 1331
Alw21I GWGCWC 1 cut(s) 1238
Alw26I GTCTC 1 cut(s) 342
AlwI GGATC 1 cut(s) 1432
Ama87I CYCGRG 1 cut(s) 652
Aor13HI TCCGGA 1 cut(s) 1031
AoxI GGCC 7 cut(s) 184, 202, 458, 777, 972, 1075, 1338
ApeKI GCWGC 6 cut(s) 264, 507, 686, 798, 893, 896
ApoI RAATTY 3 cut(s) 423, 1048, 1396
AseI ATTAAT 1 cut(s) 1442
AspLEI GCGC 1 cut(s) 566
AspS9I GGNCC 5 cut(s) 185, 458, 532, 777, 1076
AsuC2I CCSGG 1 cut(s) 501
AsuHPI GGTGA 3 cut(s) 57, 172, 918
AvaI CYCGRG 1 cut(s) 652
AvaII GGWCC 1 cut(s) 532
BaeGI GKGCMC 1 cut(s) 297
BanI GGYRCC 1 cut(s) 124
BanII GRGCYC 1 cut(s) 523
BbrPI CACGTG 1 cut(s) 1134
BbsI GAAGAC 1 cut(s) 542
Bbv12I GWGCWC 1 cut(s) 1238
BbvI GCAGC 6 cut(s) 251, 494, 673, 810, 880, 908
BccI CCATC 5 cut(s) 221, 578, 676, 695, 782
BceAI ACGGC 2 cut(s) 445, 959
BciT130I CCWGG 1 cut(s) 1086
BcnI CCSGG 1 cut(s) 501
BcoDI GTCTC 1 cut(s) 342
BfaI CTAG 1 cut(s) 756
BfmI CTRYAG 3 cut(s) 894, 1152, 1353
BfoI RGCGCY 1 cut(s) 567
BfuAI ACCTGC 1 cut(s) 866
Bme1390I CCNGG 2 cut(s) 501, 1086
Bme18I GGWCC 1 cut(s) 532
BmeT110I CYCGRG 1 cut(s) 652
BmgT120I GGNCC 5 cut(s) 185, 458, 532, 777, 1076
BmiI GGNNCC 4 cut(s) 126, 223, 778, 1282
BmrFI CCNGG 2 cut(s) 501, 1086
BmrI ACTGGG 1 cut(s) 823
BmsI GCATC 1 cut(s) 924
BmuI ACTGGG 1 cut(s) 823
BpiI GAAGAC 1 cut(s) 542
BplI GAGNNNNNCTC 2 cut(s) 1225, 1257
Bpu10I CCTNAGC 1 cut(s) 268
BpuMI CCSGG 1 cut(s) 501
Bsa29I ATCGAT 1 cut(s) 135
BsaAI YACGTR 1 cut(s) 1134
BsaJI CCNNGG 3 cut(s) 1011, 1085, 1384
BsaWI WCCGGW 1 cut(s) 1031
BsaXI ACNNNNNCTCC 2 cut(s) 336, 366
Bsc4I CCNNNNNNNGG 8 cut(s) 218, 477, 478, 658, 659, 774, 1038, 1085
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 3 cut(s) 527, 829, 1326
Bse3DI GCAATG 1 cut(s) 1144
BseAI TCCGGA 1 cut(s) 1031
BseBI CCWGG 1 cut(s) 1086
BseCI ATCGAT 1 cut(s) 135
BseDI CCNNGG 3 cut(s) 1011, 1085, 1384
BseGI GGATG 5 cut(s) 424, 589, 793, 1320, 1454
BseLI CCNNNNNNNGG 8 cut(s) 218, 477, 478, 658, 659, 774, 1038, 1085
BseMI GCAATG 1 cut(s) 1144
BseMII CTCAG 3 cut(s) 246, 282, 414
BseNI ACTGG 3 cut(s) 527, 829, 1326
BseSI GKGCMC 1 cut(s) 297
BseX3I CGGCCG 1 cut(s) 972
BseXI GCAGC 6 cut(s) 251, 494, 673, 810, 880, 908
BseYI CCCAGC 2 cut(s) 192, 1331
Bsh1285I CGRYCG 1 cut(s) 975
BshFI GGCC 7 cut(s) 186, 204, 460, 779, 974, 1077, 1340
BshNI GGYRCC 1 cut(s) 124
BshVI ATCGAT 1 cut(s) 135
BsiEI CGRYCG 1 cut(s) 975
BsiHKAI GWGCWC 1 cut(s) 1238
BsiHKCI CYCGRG 1 cut(s) 652
BsiSI CCGG 5 cut(s) 122, 183, 201, 501, 1032
BslI CCNNNNNNNGG 8 cut(s) 218, 477, 478, 658, 659, 774, 1038, 1085
BsmAI GTCTC 1 cut(s) 342
BsmI GAATGC 1 cut(s) 851
BsnI GGCC 7 cut(s) 186, 204, 460, 779, 974, 1077, 1340
BsoBI CYCGRG 1 cut(s) 652
Bsp1286I GDGCHC 3 cut(s) 297, 523, 1238
Bsp13I TCCGGA 1 cut(s) 1031
Bsp143I GATC 5 cut(s) 432, 748, 862, 1359, 1424
BspACI CCGC 8 cut(s) 14, 219, 251, 416, 485, 606, 1341, 1365
BspANI GGCC 7 cut(s) 186, 204, 460, 779, 974, 1077, 1340
BspCNI CTCAG 3 cut(s) 245, 281, 413
BspDI ATCGAT 1 cut(s) 135
BspEI TCCGGA 1 cut(s) 1031
BspHI TCATGA 1 cut(s) 1003
BspLI GGNNCC 4 cut(s) 126, 223, 778, 1282
BspMAI CTGCAG 1 cut(s) 898
BspMI ACCTGC 1 cut(s) 866
BspPI GGATC 1 cut(s) 1432
BspT107I GGYRCC 1 cut(s) 124
BsrDI GCAATG 1 cut(s) 1144
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 3 cut(s) 527, 829, 1326
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 3 cut(s) 1011, 1085, 1384
BssMI GATC 5 cut(s) 432, 748, 862, 1359, 1424
BssT1I CCWWGG 1 cut(s) 1011
Bst2UI CCWGG 1 cut(s) 1086
Bst4CI ACNGT 4 cut(s) 712, 717, 773, 1385
Bst6I CTCTTC 3 cut(s) 27, 108, 610
BstBAI YACGTR 1 cut(s) 1134
BstC8I GCNNGC 3 cut(s) 796, 891, 1234
BstDEI CTNAG 3 cut(s) 232, 268, 400
BstDSI CCRYGG 1 cut(s) 1384
BstF5I GGATG 5 cut(s) 424, 589, 793, 1320, 1454
BstH2I RGCGCY 1 cut(s) 567
BstHHI GCGC 1 cut(s) 566
BstKTI GATC 5 cut(s) 435, 751, 865, 1362, 1427
BstMAI GTCTC 1 cut(s) 342
BstMBI GATC 5 cut(s) 432, 748, 862, 1359, 1424
BstMCI CGRYCG 1 cut(s) 975
BstMWI GCNNNNNNNGC 6 cut(s) 270, 491, 605, 695, 1238, 1337
BstNI CCWGG 1 cut(s) 1086
BstNSI RCATGY 1 cut(s) 693
BstSCI CCNGG 2 cut(s) 499, 1084
BstSFI CTRYAG 3 cut(s) 894, 1152, 1353
BstSLI GKGCMC 1 cut(s) 297
BstV1I GCAGC 6 cut(s) 251, 494, 673, 810, 880, 908
BstV2I GAAGAC 1 cut(s) 542
BstX2I RGATCY 1 cut(s) 1424
BstXI CCANNNNNNTGG 1 cut(s) 836
BstYI RGATCY 1 cut(s) 1424
BstZI CGGCCG 1 cut(s) 972
Bsu15I ATCGAT 1 cut(s) 135
BsuRI GGCC 7 cut(s) 186, 204, 460, 779, 974, 1077, 1340
BsuTUI ATCGAT 1 cut(s) 135
BtgI CCRYGG 1 cut(s) 1384
BtsCI GGATG 5 cut(s) 424, 589, 793, 1320, 1454
BtsI GCAGTG 2 cut(s) 1032, 1236
BtsIMutI CAGTG 5 cut(s) 113, 534, 1032, 1236, 1319
BveI ACCTGC 1 cut(s) 866
Cac8I GCNNGC 3 cut(s) 796, 891, 1234
CciI TCATGA 1 cut(s) 1003
CfoI GCGC 1 cut(s) 566
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 5 cut(s) 185, 458, 532, 777, 1076
ClaI ATCGAT 1 cut(s) 135
Csp6I GTAC 3 cut(s) 209, 713, 879
CviAII CATG 8 cut(s) 206, 690, 836, 912, 1004, 1037, 1180, 1187
CviQI GTAC 3 cut(s) 209, 713, 879
DdeI CTNAG 3 cut(s) 232, 268, 400
DpnI GATC 5 cut(s) 434, 750, 864, 1361, 1426
DpnII GATC 5 cut(s) 432, 748, 862, 1359, 1424
EaeI YGGCCR 3 cut(s) 202, 972, 1338
EagI CGGCCG 1 cut(s) 972
Eam1104I CTCTTC 3 cut(s) 27, 108, 610
EarI CTCTTC 3 cut(s) 27, 108, 610
EclXI CGGCCG 1 cut(s) 972
Eco130I CCWWGG 1 cut(s) 1011
Eco24I GRGCYC 1 cut(s) 523
Eco32I GATATC 1 cut(s) 924
Eco47I GGWCC 1 cut(s) 532
Eco52I CGGCCG 1 cut(s) 972
Eco72I CACGTG 1 cut(s) 1134
Eco88I CYCGRG 1 cut(s) 652
EcoO109I RGGNCCY 1 cut(s) 777
EcoRII CCWGG 1 cut(s) 1084
EcoRV GATATC 1 cut(s) 924
EcoT14I CCWWGG 1 cut(s) 1011
EcoT22I ATGCAT 1 cut(s) 917
EcoT38I GRGCYC 1 cut(s) 523
ErhI CCWWGG 1 cut(s) 1011
FaeI CATG 8 cut(s) 209, 693, 839, 915, 1007, 1040, 1183, 1190
FatI CATG 8 cut(s) 205, 689, 835, 911, 1003, 1036, 1179, 1186
FauNDI CATATG 1 cut(s) 601
FokI GGATG 5 cut(s) 431, 596, 800, 1327, 1441
FriOI GRGCYC 1 cut(s) 523
FspBI CTAG 1 cut(s) 756
GlaI GCGC 1 cut(s) 565
GsaI CCCAGC 2 cut(s) 196, 1335
HaeII RGCGCY 1 cut(s) 567
HaeIII GGCC 7 cut(s) 186, 204, 460, 779, 974, 1077, 1340
HapII CCGG 5 cut(s) 122, 183, 201, 501, 1032
HhaI GCGC 1 cut(s) 566
Hin1II CATG 8 cut(s) 209, 693, 839, 915, 1007, 1040, 1183, 1190
Hin6I GCGC 1 cut(s) 564
HinP1I GCGC 1 cut(s) 564
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HindIII AAGCTT 2 cut(s) 806, 1205
HinfI GANTC 7 cut(s) 118, 244, 283, 474, 1160, 1183, 1219
HpaI GTTAAC 1 cut(s) 382
HpaII CCGG 5 cut(s) 122, 183, 201, 501, 1032
HphI GGTGA 3 cut(s) 57, 172, 918
Hpy166II GTNNAC 2 cut(s) 382, 532
Hpy188I TCNGA 3 cut(s) 437, 1128, 1429
Hpy188III TCNNGA 5 cut(s) 919, 991, 1004, 1032, 1273
Hpy8I GTNNAC 2 cut(s) 382, 532
HpyAV CCTTC 3 cut(s) 55, 198, 1340
HpyCH4III ACNGT 4 cut(s) 712, 717, 773, 1385
HpyCH4IV ACGT 2 cut(s) 291, 1133
HpyF10VI GCNNNNNNNGC 6 cut(s) 270, 491, 605, 695, 1238, 1337
HpyF3I CTNAG 3 cut(s) 232, 268, 400
HpySE526I ACGT 2 cut(s) 291, 1133
Hsp92II CATG 8 cut(s) 209, 693, 839, 915, 1007, 1040, 1183, 1190
HspAI GCGC 1 cut(s) 564
Kpn2I TCCGGA 1 cut(s) 1031
KspAI GTTAAC 1 cut(s) 382
Kzo9I GATC 5 cut(s) 432, 748, 862, 1359, 1424
LmnI GCTCC 4 cut(s) 227, 344, 791, 1211
Lsp1109I GCAGC 6 cut(s) 251, 494, 673, 810, 880, 908
LweI GCATC 1 cut(s) 924
MaeI CTAG 1 cut(s) 756
MaeII ACGT 2 cut(s) 291, 1133
MaeIII GTNAC 1 cut(s) 1317
MalI GATC 5 cut(s) 434, 750, 864, 1361, 1426
MboI GATC 5 cut(s) 432, 748, 862, 1359, 1424
MboII GAAGA 5 cut(s) 14, 16, 95, 542, 627
MflI RGATCY 1 cut(s) 1424
MhlI GDGCHC 3 cut(s) 297, 523, 1238
MluCI AATT 4 cut(s) 423, 575, 1048, 1396
MlyI GAGTC 1 cut(s) 1192
MmeI TCCRAC 3 cut(s) 607, 664, 1129
Mph1103I ATGCAT 1 cut(s) 917
MroI TCCGGA 1 cut(s) 1031
MseI TTAA 4 cut(s) 381, 1068, 1145, 1442
MslI CAYNNNNRTG 2 cut(s) 840, 1041
MspA1I CMGCKG 2 cut(s) 219, 1365
MspI CCGG 5 cut(s) 122, 183, 201, 501, 1032
MspR9I CCNGG 2 cut(s) 501, 1086
Mva1269I GAATGC 1 cut(s) 851
MvaI CCWGG 1 cut(s) 1086
MwoI GCNNNNNNNGC 6 cut(s) 270, 491, 605, 695, 1238, 1337
NciI CCSGG 1 cut(s) 501
NdeI CATATG 1 cut(s) 601
NdeII GATC 5 cut(s) 432, 748, 862, 1359, 1424
NlaIII CATG 8 cut(s) 209, 693, 839, 915, 1007, 1040, 1183, 1190
NlaIV GGNNCC 4 cut(s) 126, 223, 778, 1282
NmuCI GTSAC 1 cut(s) 1317
NsiI ATGCAT 1 cut(s) 917
NspI RCATGY 1 cut(s) 693
PagI TCATGA 1 cut(s) 1003
PctI GAATGC 1 cut(s) 851
PfeI GAWTC 6 cut(s) 118, 244, 283, 474, 1160, 1219
PflMI CCANNNNNTGG 1 cut(s) 1085
PleI GAGTC 1 cut(s) 1191
PmaCI CACGTG 1 cut(s) 1134
PmlI CACGTG 1 cut(s) 1134
PpsI GAGTC 1 cut(s) 1191
Ppu21I YACGTR 1 cut(s) 1134
PshBI ATTAAT 1 cut(s) 1442
Psp6I CCWGG 1 cut(s) 1084
PspCI CACGTG 1 cut(s) 1134
PspFI CCCAGC 2 cut(s) 192, 1331
PspGI CCWGG 1 cut(s) 1084
PspN4I GGNNCC 4 cut(s) 126, 223, 778, 1282
PspPI GGNCC 5 cut(s) 185, 458, 532, 777, 1076
PstI CTGCAG 1 cut(s) 898
PsuI RGATCY 1 cut(s) 1424
RsaI GTAC 3 cut(s) 210, 714, 880
RsaNI GTAC 3 cut(s) 209, 713, 879
RseI CAYNNNNRTG 2 cut(s) 840, 1041
SaqAI TTAA 4 cut(s) 381, 1068, 1145, 1442
Sau3AI GATC 5 cut(s) 432, 748, 862, 1359, 1424
Sau96I GGNCC 5 cut(s) 185, 458, 532, 777, 1076
SchI GAGTC 1 cut(s) 1192
ScrFI CCNGG 2 cut(s) 501, 1086
SduI GDGCHC 3 cut(s) 297, 523, 1238
SfaNI GCATC 1 cut(s) 924
SfcI CTRYAG 3 cut(s) 894, 1152, 1353
SinI GGWCC 1 cut(s) 532
SmiMI CAYNNNNRTG 2 cut(s) 840, 1041
Sse9I AATT 4 cut(s) 423, 575, 1048, 1396
SsiI CCGC 8 cut(s) 14, 219, 251, 416, 485, 606, 1341, 1365
SspI AATATT 1 cut(s) 514
SspMI CTAG 1 cut(s) 756
StyD4I CCNGG 2 cut(s) 499, 1084
StyI CCWWGG 1 cut(s) 1011
TaaI ACNGT 4 cut(s) 712, 717, 773, 1385
TaiI ACGT 2 cut(s) 294, 1136
TaqI TCGA 4 cut(s) 135, 306, 523, 920
TasI AATT 4 cut(s) 423, 575, 1048, 1396
TatI WGTACW 1 cut(s) 712
TauI GCSGC 5 cut(s) 17, 222, 254, 608, 1343
TfiI GAWTC 6 cut(s) 118, 244, 283, 474, 1160, 1219
Tru1I TTAA 4 cut(s) 381, 1068, 1145, 1442
Tru9I TTAA 4 cut(s) 381, 1068, 1145, 1442
TscAI CASTG 5 cut(s) 120, 534, 1032, 1243, 1326
TseFI GTSAC 1 cut(s) 1317
TseI GCWGC 6 cut(s) 264, 507, 686, 798, 893, 896
Tsp45I GTSAC 1 cut(s) 1317
TspDTI ATGAA 3 cut(s) 17, 435, 1421
TspGWI ACGGA 1 cut(s) 683
TspRI CASTG 5 cut(s) 120, 534, 1032, 1243, 1326
Van91I CCANNNNNTGG 1 cut(s) 1085
VpaK11BI GGWCC 1 cut(s) 532
VspI ATTAAT 1 cut(s) 1442
XapI RAATTY 3 cut(s) 423, 1048, 1396
XceI RCATGY 1 cut(s) 693
XspI CTAG 1 cut(s) 756
Zsp2I ATGCAT 1 cut(s) 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.