pycom10g07050

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000590
Physical Location & Seq
Forward (+)
8257680 .. 8258987
1308 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1086 bp
ATGGGAGAGATTAATCCAGCTTTCATCCAAGATCCCGAGCACCGGCCAAAACTCTCGATCATCGAAGCTGATGGCATACCATTAATTGACCTGTCTCCCGTAAACTCCGCAGACAACTCTTCTGATCCAAAAGCCCTTGAAAAACTTGTTAGAGAGATCGGCAATGCATGCAAAGACTGGGGGTTCTTCCAAGTGATCAACCATGGAGTACAACTGGATACGCTCGGAAAGACTGAGGCTGCTGCTCGGAAGTTCTTTGATCTGCCTTCGGAGGAGAAAAGGCGGATTTGGAGGGATGAAAAAAGTGTGTTGGGTTACTACGATACTGAGCATACCAAGAACGTACGAGACTGGAAGGAGGTGTTCGATTTCACAGTGGAGGAACCTATGTTAATGCCCGTTTCAGCTGATCCTGCGGACAACGAAGAGATAGAGTGGACTAACCAATGGCCTGAGCAACTTCCAGAACTAAGGGTGTTGTGTGATGAATACGCTCGAGAAGTAGAAAAACTAGCTCTCAAGTTGTTGGGACTTATTGCCTTGAGCCTAGGCTTGCCAGAAAACAGGTTCAACAGCTACTTCAAAGACCAGACAAGTTTTATCAGGCTCAATCACTATCCACCTTGCCCTTCCCCTCAGTTAGCTCTTGGTGTTGGTCGCCACAAGGACAGCGGTGCTCTAACCGTGCTGTCTCAGGATGATGTCGGAGGACTGGAGGTGAAGAGAAAAGCAGACGGAGAGTGGATTCCGGTTAAACCCACCTCAAATGCCTATATCATCAATGTTGGTGACATTCTTCAGGTTCCCCTCTACAAACTTAATACTAAGTTTGGAGCAATGATAGATATGAGTGTGGAACACAGAGCGGTGGTGAATACGGAGAAGGAAAGGTTTTCCATTCCGTTCTTCGTCAACCCCGCACACTACACCGTGGTCATGCCCATGGAGGAGCTGACAGATGAACAGAACCCGGCGAAATACAAGCCGTACAACTGGGGCAAGTTCTTGAGTCACCGAAAGCTGACTAATTTCAAGAAACACAGTGCTGAAAACATCCAGGTTTCTCATTTCAGGGTAGAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

362

Amino Acids

41.28

Weight (kDa)

5.35

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 26 - 153 3.8e-32 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 199 - 306 7.6e-26 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19000 AT3G19000
fragaria_vesca FvH4_2g07071 FvH4_2g07080 FvH4_2g07080 FvH4_2g07081 FvH4_2g07082 FvH4_2g07082
malus_domestica MD05G1074000.v1.1 MD10G1088100.v1.1 MD10G1088200.v1.1 MD14G1207600.v1.1
prunus_persica Prupe.8G114100_v2.0.a1 Prupe.8G114200_v2.0.a1 Prupe.8G114300_v2.0.a1 Prupe.8G114500_v2.0.a1 Prupe.8G114800_v2.0.a1 Prupe.8G115100_v2.0.a1
pyrus_communis pycom05g06640 pycom05g06660 pycom05g06670 pycom10g07050
rosa_chinensis RchiOBHm_Chr6g0244141 RchiOBHm_Chr6g0259511 RchiOBHm_Chr6g0259521 RchiOBHm_Chr6g0259531 RchiOBHm_Chr6g0259551 RchiOBHm_Chr6g0259561
rosa_laevigata RLG00000014522 RLG00000014523 RLG00000014524 RLG00000014525 RLG00000014526 RLG00000014527
rosa_multiflora Rmu_ssc0000050.1_g000019 Rmu_ssc0000050.1_g000020 Rmu_ssc0000050.1_g000026 Rmu_ssc0000050.1_g000027 Rmu_ssc0000050.1_g000028
rosa_roxburghii Rroxscaffold_7G00206780 Rroxscaffold_7G00206790 Rroxscaffold_7G00206850 Rroxscaffold_7G00206860 Rroxscaffold_7G00206870 Rroxscaffold_7G00206880
rosa_rugosa Rorug05G0589100 Rorug05G0589200.1 Rorug05G0589300 Rorug05G0589400 Rorug05G0589500 Rorug05G0589600
rosa_samantha Rh4CG096000 Rh5BG231700 Rh6AG104900 Rh6AG105000 Rh6AG105100 Rh6AG105500 Rh6AG105600 Rh6BG098500 Rh6BG098700 Rh6BG098800 Rh6BG099000 Rh6BG099100 Rh6CG094200 Rh6CG094300 Rh6CG094400 Rh6CG094500 Rh6CG094600 Rh6DG088300 Rh6DG088400 Rh6DG088500 Rh6DG088600 Rh6DG088700
rosa_wichuraiana Rw0G014270 Rw0G014280 Rw0G014290 Rw6G009080 Rw6G009090 Rw6G009100 Rw6G009110 Rw6G009120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 866
AciI CCGC 6 cut(s) 108, 283, 416, 672, 866, 918
AclWI GGATC 3 cut(s) 26, 119, 404
AcoI YGGCCR 1 cut(s) 44
AcuI CTGAAG 1 cut(s) 782
AfaI GTAC 3 cut(s) 210, 345, 989
AfiI CCNNNNNNNGG 1 cut(s) 42
AgsI TTSAA 4 cut(s) 140, 571, 583, 1033
AjnI CCWGG 1 cut(s) 1056
AluBI AGCT 8 cut(s) 20, 68, 407, 515, 576, 644, 952, 1021
AluI AGCT 8 cut(s) 20, 68, 407, 515, 576, 644, 952, 1021
Alw21I GWGCWC 2 cut(s) 42, 679
Alw26I GTCTC 3 cut(s) 99, 342, 696
AlwI GGATC 3 cut(s) 26, 119, 404
Ama87I CYCGRG 2 cut(s) 35, 495
AoxI GGCC 2 cut(s) 44, 449
ApeKI GCWGC 2 cut(s) 239, 242
AseI ATTAAT 2 cut(s) 12, 83
AspA2I CCTAGG 1 cut(s) 547
AsuC2I CCSGG 1 cut(s) 971
AsuHPI GGTGA 4 cut(s) 730, 800, 883, 1004
AvaI CYCGRG 2 cut(s) 35, 495
AvrII CCTAGG 1 cut(s) 547
Bbv12I GWGCWC 2 cut(s) 42, 679
BbvI GCAGC 2 cut(s) 226, 229
BccI CCATC 1 cut(s) 65
BceAI ACGGC 1 cut(s) 970
BciT130I CCWGG 1 cut(s) 1058
BciVI GTATCC 1 cut(s) 211
BclI TGATCA 1 cut(s) 195
BcnI CCSGG 1 cut(s) 971
BcoDI GTCTC 3 cut(s) 99, 342, 696
BfaI CTAG 2 cut(s) 512, 548
BfuI GTATCC 1 cut(s) 211
BisI GCNGC 2 cut(s) 240, 243
BlnI CCTAGG 1 cut(s) 547
BlsI GCNGC 2 cut(s) 241, 244
Bme1390I CCNGG 2 cut(s) 971, 1058
BmeT110I CYCGRG 2 cut(s) 35, 495
BmiI GGNNCC 2 cut(s) 384, 804
BmrFI CCNGG 2 cut(s) 971, 1058
BmrI ACTGGG 2 cut(s) 187, 1003
BmuI ACTGGG 2 cut(s) 187, 1003
BpmI CTGGAG 1 cut(s) 734
Bpu10I CCTNAGC 1 cut(s) 453
BpuEI CTTGAG 3 cut(s) 503, 562, 1027
BpuMI CCSGG 1 cut(s) 971
BsaJI CCNNGG 4 cut(s) 202, 547, 930, 942
BsaWI WCCGGW 1 cut(s) 748
Bsc4I CCNNNNNNNGG 1 cut(s) 42
Bse118I RCCGGY 1 cut(s) 42
Bse1I ACTGG 5 cut(s) 182, 219, 356, 717, 998
Bse3DI GCAATG 2 cut(s) 169, 843
BseBI CCWGG 1 cut(s) 1058
BseDI CCNNGG 4 cut(s) 202, 547, 930, 942
BseGI GGATG 4 cut(s) 24, 301, 703, 1053
BseLI CCNNNNNNNGG 1 cut(s) 42
BseMI GCAATG 2 cut(s) 169, 843
BseMII CTCAG 5 cut(s) 225, 318, 444, 650, 707
BseNI ACTGG 5 cut(s) 182, 219, 356, 717, 998
BseRI GAGGAG 2 cut(s) 287, 962
BseXI GCAGC 2 cut(s) 226, 229
BshFI GGCC 2 cut(s) 46, 451
BsiHKAI GWGCWC 2 cut(s) 42, 679
BsiHKCI CYCGRG 2 cut(s) 35, 495
BsiSI CCGG 3 cut(s) 43, 749, 971
BsiWI CGTACG 1 cut(s) 343
BslFI GGGAC 1 cut(s) 543
BslI CCNNNNNNNGG 1 cut(s) 42
BsmAI GTCTC 3 cut(s) 99, 342, 696
BsmFI GGGAC 1 cut(s) 543
BsnI GGCC 2 cut(s) 46, 451
BsoBI CYCGRG 2 cut(s) 35, 495
Bsp1286I GDGCHC 2 cut(s) 42, 679
Bsp143I GATC 7 cut(s) 31, 57, 124, 156, 195, 259, 409
Bsp19I CCATGG 2 cut(s) 202, 942
BspACI CCGC 6 cut(s) 108, 283, 416, 672, 866, 918
BspANI GGCC 2 cut(s) 46, 451
BspCNI CTCAG 5 cut(s) 226, 319, 445, 649, 706
BspLI GGNNCC 2 cut(s) 384, 804
BspPI GGATC 3 cut(s) 26, 119, 404
BsrBI CCGCTC 1 cut(s) 866
BsrDI GCAATG 2 cut(s) 169, 843
BsrFI RCCGGY 1 cut(s) 42
BsrI ACTGG 5 cut(s) 182, 219, 356, 717, 998
BssAI RCCGGY 1 cut(s) 42
BssECI CCNNGG 4 cut(s) 202, 547, 930, 942
BssMI GATC 7 cut(s) 31, 57, 124, 156, 195, 259, 409
BssT1I CCWWGG 3 cut(s) 202, 547, 942
Bst2UI CCWGG 1 cut(s) 1058
Bst4CI ACNGT 4 cut(s) 376, 685, 931, 1043
Bst6I CTCTTC 3 cut(s) 124, 420, 716
BstAPI GCANNNNNTGC 1 cut(s) 168
BstC8I GCNNGC 2 cut(s) 169, 554
BstDEI CTNAG 7 cut(s) 234, 327, 453, 470, 636, 693, 825
BstDSI CCRYGG 3 cut(s) 202, 930, 942
BstF5I GGATG 4 cut(s) 24, 301, 703, 1053
BstKTI GATC 7 cut(s) 34, 60, 127, 159, 198, 262, 412
BstMAI GTCTC 3 cut(s) 99, 342, 696
BstMBI GATC 7 cut(s) 31, 57, 124, 156, 195, 259, 409
BstMWI GCNNNNNNNGC 2 cut(s) 168, 413
BstNI CCWGG 1 cut(s) 1058
BstNSI RCATGY 1 cut(s) 171
BstSCI CCNGG 2 cut(s) 969, 1056
BstV1I GCAGC 2 cut(s) 226, 229
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
BsuI GTATCC 1 cut(s) 211
BsuRI GGCC 2 cut(s) 46, 451
BtgI CCRYGG 3 cut(s) 202, 930, 942
BtsCI GGATG 4 cut(s) 24, 301, 703, 1053
BtsIMutI CAGTG 2 cut(s) 381, 1048
Cac8I GCNNGC 2 cut(s) 169, 554
Cfr10I RCCGGY 1 cut(s) 42
Csp6I GTAC 3 cut(s) 209, 344, 988
CviAII CATG 4 cut(s) 168, 203, 937, 943
CviQI GTAC 3 cut(s) 209, 344, 988
DdeI CTNAG 7 cut(s) 234, 327, 453, 470, 636, 693, 825
DpnI GATC 7 cut(s) 33, 59, 126, 158, 197, 261, 411
DpnII GATC 7 cut(s) 31, 57, 124, 156, 195, 259, 409
EaeI YGGCCR 1 cut(s) 44
Eam1104I CTCTTC 3 cut(s) 124, 420, 716
EarI CTCTTC 3 cut(s) 124, 420, 716
EciI GGCGGA 1 cut(s) 298
Eco130I CCWWGG 3 cut(s) 202, 547, 942
Eco57I CTGAAG 1 cut(s) 782
Eco88I CYCGRG 2 cut(s) 35, 495
EcoRII CCWGG 1 cut(s) 1056
EcoT14I CCWWGG 3 cut(s) 202, 547, 942
EcoT22I ATGCAT 1 cut(s) 169
ErhI CCWWGG 3 cut(s) 202, 547, 942
FaeI CATG 4 cut(s) 171, 206, 940, 946
FaiI YATR 9 cut(s) 77, 169, 204, 333, 389, 774, 848, 938, 944
FaqI GGGAC 1 cut(s) 543
FatI CATG 4 cut(s) 167, 202, 936, 942
FauI CCCGC 1 cut(s) 925
FbaI TGATCA 1 cut(s) 195
Fnu4HI GCNGC 2 cut(s) 240, 243
FokI GGATG 4 cut(s) 11, 308, 710, 1040
Fsp4HI GCNGC 2 cut(s) 240, 243
FspBI CTAG 2 cut(s) 512, 548
GluI GCNGC 2 cut(s) 240, 243
GsuI CTGGAG 1 cut(s) 734
HaeIII GGCC 2 cut(s) 46, 451
HapII CCGG 3 cut(s) 43, 749, 971
Hin1II CATG 4 cut(s) 171, 206, 940, 946
HincII GTYRAC 1 cut(s) 913
HindII GTYRAC 1 cut(s) 913
HinfI GANTC 2 cut(s) 745, 1009
HpaII CCGG 3 cut(s) 43, 749, 971
HphI GGTGA 4 cut(s) 730, 800, 883, 1004
Hpy166II GTNNAC 3 cut(s) 103, 438, 913
Hpy188I TCNGA 5 cut(s) 124, 227, 249, 271, 707
Hpy188III TCNNGA 7 cut(s) 35, 55, 464, 497, 695, 1006, 1033
Hpy8I GTNNAC 3 cut(s) 103, 438, 913
HpyAV CCTTC 4 cut(s) 276, 349, 639, 877
HpyCH4III ACNGT 4 cut(s) 376, 685, 931, 1043
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 2 cut(s) 167, 171
HpyF10VI GCNNNNNNNGC 2 cut(s) 168, 413
HpyF3I CTNAG 7 cut(s) 234, 327, 453, 470, 636, 693, 825
HpySE526I ACGT 1 cut(s) 342
Hsp92II CATG 4 cut(s) 171, 206, 940, 946
Ksp22I TGATCA 1 cut(s) 195
Kzo9I GATC 7 cut(s) 31, 57, 124, 156, 195, 259, 409
LmnI GCTCC 2 cut(s) 833, 949
Lsp1109I GCAGC 2 cut(s) 226, 229
MaeI CTAG 2 cut(s) 512, 548
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 3 cut(s) 314, 788, 1010
MalI GATC 7 cut(s) 33, 59, 126, 158, 197, 261, 411
MbiI CCGCTC 1 cut(s) 866
MboI GATC 7 cut(s) 31, 57, 124, 156, 195, 259, 409
MboII GAAGA 6 cut(s) 111, 178, 437, 733, 788, 898
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 2 cut(s) 42, 679
MluCI AATT 2 cut(s) 84, 1027
MlyI GAGTC 1 cut(s) 1018
MmeI TCCRAC 1 cut(s) 685
Mph1103I ATGCAT 1 cut(s) 169
MseI TTAA 5 cut(s) 12, 83, 392, 753, 819
MslI CAYNNNNRTG 1 cut(s) 941
MspA1I CMGCKG 2 cut(s) 407, 672
MspI CCGG 3 cut(s) 43, 749, 971
MspR9I CCNGG 2 cut(s) 971, 1058
MvaI CCWGG 1 cut(s) 1058
MwoI GCNNNNNNNGC 2 cut(s) 168, 413
NciI CCSGG 1 cut(s) 971
NcoI CCATGG 2 cut(s) 202, 942
NdeII GATC 7 cut(s) 31, 57, 124, 156, 195, 259, 409
NlaIII CATG 4 cut(s) 171, 206, 940, 946
NlaIV GGNNCC 2 cut(s) 384, 804
NmuCI GTSAC 2 cut(s) 788, 1010
NsiI ATGCAT 1 cut(s) 169
NspI RCATGY 1 cut(s) 171
PaeI GCATGC 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 495
PfeI GAWTC 1 cut(s) 745
Pfl23II CGTACG 1 cut(s) 343
PkrI GCNGC 2 cut(s) 241, 244
PleI GAGTC 1 cut(s) 1017
PpsI GAGTC 1 cut(s) 1017
PshBI ATTAAT 2 cut(s) 12, 83
Psp6I CCWGG 1 cut(s) 1056
PspGI CCWGG 1 cut(s) 1056
PspLI CGTACG 1 cut(s) 343
PspN4I GGNNCC 2 cut(s) 384, 804
PsuI RGATCY 1 cut(s) 31
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 3 cut(s) 210, 345, 989
RsaNI GTAC 3 cut(s) 209, 344, 988
RseI CAYNNNNRTG 1 cut(s) 941
SaqAI TTAA 5 cut(s) 12, 83, 392, 753, 819
SatI GCNGC 2 cut(s) 240, 243
Sau3AI GATC 7 cut(s) 31, 57, 124, 156, 195, 259, 409
SchI GAGTC 1 cut(s) 1018
ScrFI CCNGG 2 cut(s) 971, 1058
SduI GDGCHC 2 cut(s) 42, 679
Sfr274I CTCGAG 1 cut(s) 495
SlaI CTCGAG 1 cut(s) 495
SmiMI CAYNNNNRTG 1 cut(s) 941
SmlI CTYRAG 4 cut(s) 495, 518, 541, 1006
SmoI CTYRAG 4 cut(s) 495, 518, 541, 1006
SphI GCATGC 1 cut(s) 171
Sse9I AATT 2 cut(s) 84, 1027
SsiI CCGC 6 cut(s) 108, 283, 416, 672, 866, 918
SspMI CTAG 2 cut(s) 512, 548
StyD4I CCNGG 2 cut(s) 969, 1056
StyI CCWWGG 3 cut(s) 202, 547, 942
TaaI ACNGT 4 cut(s) 376, 685, 931, 1043
TaiI ACGT 1 cut(s) 345
TaqI TCGA 4 cut(s) 56, 63, 366, 496
TasI AATT 2 cut(s) 84, 1027
TatI WGTACW 1 cut(s) 208
TfiI GAWTC 1 cut(s) 745
Tru1I TTAA 5 cut(s) 12, 83, 392, 753, 819
Tru9I TTAA 5 cut(s) 12, 83, 392, 753, 819
TscAI CASTG 2 cut(s) 381, 1048
TseFI GTSAC 2 cut(s) 788, 1010
TseI GCWGC 2 cut(s) 239, 242
Tsp45I GTSAC 2 cut(s) 788, 1010
TspDTI ATGAA 4 cut(s) 13, 312, 501, 975
TspGWI ACGGA 3 cut(s) 750, 891, 893
TspRI CASTG 2 cut(s) 381, 1048
VspI ATTAAT 2 cut(s) 12, 83
XceI RCATGY 1 cut(s) 171
XhoI CTCGAG 1 cut(s) 495
XmaJI CCTAGG 1 cut(s) 547
XspI CTAG 2 cut(s) 512, 548
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.