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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1325 |
| AarI | CACCTGC | 1 cut(s) | 441 |
| Acc16I | TGCGCA | 3 cut(s) | 444, 3897, 4892 |
| Acc36I | ACCTGC | 1 cut(s) | 441 |
| Acc65I | GGTACC | 1 cut(s) | 2101 |
| AccB1I | GGYRCC | 7 cut(s) | 1698, 1716, 2101, 3856, 4603, 4837, 4860 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 2635, 3345 |
| AccI | GTMKAC | 3 cut(s) | 756, 4451, 4673 |
| AccII | CGCG | 4 cut(s) | 406, 548, 619, 639 |
| AccIII | TCCGGA | 1 cut(s) | 1233 |
| AciI | CCGC | 7 cut(s) | 637, 1686, 1841, 3028, 4159, 4490, 4518 |
| AclI | AACGTT | 1 cut(s) | 3520 |
| AcoI | YGGCCR | 3 cut(s) | 1762, 2887, 3241 |
| AcsI | RAATTY | 9 cut(s) | 180, 625, 1282, 1787, 3097, 3233, 3540, 3798, 3877 |
| AcuI | CTGAAG | 2 cut(s) | 1791, 3622 |
| AcyI | GRCGYC | 4 cut(s) | 3857, 4007, 4604, 4838 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 1074, 2275, 2635, 2921, 3212, 3345, 4549, 4939 |
| AflII | CTTAAG | 1 cut(s) | 4745 |
| AflIII | ACRYGT | 1 cut(s) | 3459 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 4546 |
| AhlI | ACTAGT | 1 cut(s) | 130 |
| AjnI | CCWGG | 4 cut(s) | 1014, 2097, 3205, 4667 |
| AleI | CACNNNNGTG | 1 cut(s) | 3297 |
| AluBI | AGCT | 6 cut(s) | 858, 1181, 1553, 1940, 2251, 2679 |
| AluI | AGCT | 6 cut(s) | 858, 1181, 1553, 1940, 2251, 2679 |
| Alw21I | GWGCWC | 4 cut(s) | 539, 1143, 3664, 4598 |
| Alw26I | GTCTC | 3 cut(s) | 2030, 2287, 2981 |
| Alw44I | GTGCAC | 2 cut(s) | 535, 3660 |
| Ama87I | CYCGRG | 3 cut(s) | 2033, 2058, 4578 |
| Aor13HI | TCCGGA | 1 cut(s) | 1233 |
| AoxI | GGCC | 9 cut(s) | 569, 1018, 1251, 1295, 1762, 2419, 2887, 3241, 4915 |
| ApaLI | GTGCAC | 2 cut(s) | 535, 3660 |
| ApeKI | GCWGC | 3 cut(s) | 631, 4514, 4520 |
| ApoI | RAATTY | 9 cut(s) | 180, 625, 1282, 1787, 3097, 3233, 3540, 3798, 3877 |
| AseI | ATTAAT | 6 cut(s) | 1749, 1968, 2454, 2574, 3080, 3762 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 3555, 4900 |
| Asp718I | GGTACC | 1 cut(s) | 2101 |
| AspS9I | GGNCC | 8 cut(s) | 106, 1018, 1236, 1296, 3140, 4540, 4574, 4916 |
| AsuC2I | CCSGG | 1 cut(s) | 4589 |
| AvaI | CYCGRG | 3 cut(s) | 2033, 2058, 4578 |
| AvaII | GGWCC | 5 cut(s) | 106, 1236, 3140, 4540, 4574 |
| AxyI | CCTNAGG | 1 cut(s) | 4401 |
| BaeGI | GKGCMC | 6 cut(s) | 539, 1701, 3034, 3664, 4255, 4863 |
| BalI | TGGCCA | 3 cut(s) | 1764, 2889, 3243 |
| BamHI | GGATCC | 1 cut(s) | 4638 |
| BanI | GGYRCC | 7 cut(s) | 1698, 1716, 2101, 3856, 4603, 4837, 4860 |
| BanII | GRGCYC | 1 cut(s) | 1340 |
| BbsI | GAAGAC | 4 cut(s) | 2060, 2825, 3501, 4899 |
| Bbv12I | GWGCWC | 4 cut(s) | 539, 1143, 3664, 4598 |
| BbvI | GCAGC | 3 cut(s) | 618, 4501, 4507 |
| BccI | CCATC | 8 cut(s) | 117, 263, 894, 1751, 2629, 2679, 3261, 4640 |
| BceAI | ACGGC | 5 cut(s) | 542, 633, 970, 1310, 3844 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 526, 560, 3994, 4028 |
| BciT130I | CCWGG | 4 cut(s) | 1016, 2099, 3207, 4669 |
| BcnI | CCSGG | 1 cut(s) | 4589 |
| BcoDI | GTCTC | 3 cut(s) | 2030, 2287, 2981 |
| BcuI | ACTAGT | 1 cut(s) | 130 |
| BfoI | RGCGCY | 3 cut(s) | 3860, 4607, 4841 |
| BfrI | CTTAAG | 1 cut(s) | 4745 |
| BfuAI | ACCTGC | 1 cut(s) | 441 |
| BisI | GCNGC | 5 cut(s) | 632, 637, 4515, 4518, 4521 |
| BlsI | GCNGC | 5 cut(s) | 633, 638, 4516, 4519, 4522 |
| Bme1390I | CCNGG | 5 cut(s) | 1016, 2099, 3207, 4589, 4669 |
| Bme18I | GGWCC | 5 cut(s) | 106, 1236, 3140, 4540, 4574 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 4546 |
| BmeT110I | CYCGRG | 3 cut(s) | 2033, 2058, 4578 |
| BmgT120I | GGNCC | 8 cut(s) | 106, 1018, 1236, 1296, 3140, 4540, 4574, 4916 |
| BmrFI | CCNGG | 5 cut(s) | 1016, 2099, 3207, 4589, 4669 |
| BmsI | GCATC | 4 cut(s) | 432, 2070, 3934, 4640 |
| BpiI | GAAGAC | 4 cut(s) | 2060, 2825, 3501, 4899 |
| BplI | GAGNNNNNCTC | 8 cut(s) | 1125, 1157, 1165, 1197, 1215, 1247, 1686, 1718 |
| BpmI | CTGGAG | 2 cut(s) | 2294, 4512 |
| BpuEI | CTTGAG | 3 cut(s) | 2000, 2295, 4640 |
| BpuMI | CCSGG | 1 cut(s) | 4589 |
| Bsa29I | ATCGAT | 3 cut(s) | 112, 1417, 3689 |
| BsaAI | YACGTR | 3 cut(s) | 530, 2369, 4951 |
| BsaBI | GATNNNNATC | 5 cut(s) | 1410, 1827, 2211, 3357, 3567 |
| BsaHI | GRCGYC | 4 cut(s) | 3857, 4007, 4604, 4838 |
| BsaI | GGTCTC | 2 cut(s) | 2030, 2287 |
| BsaJI | CCNNGG | 6 cut(s) | 935, 1015, 1248, 3205, 4587, 4912 |
| BsaWI | WCCGGW | 3 cut(s) | 1233, 2647, 3472 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1835, 1865 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 1074, 2275, 2635, 2921, 3212, 3345, 4549, 4939 |
| Bse118I | RCCGGY | 1 cut(s) | 4600 |
| Bse21I | CCTNAGG | 1 cut(s) | 4401 |
| Bse3DI | GCAATG | 6 cut(s) | 372, 1911, 2657, 4010, 4080, 4356 |
| Bse8I | GATNNNNATC | 5 cut(s) | 1410, 1827, 2211, 3357, 3567 |
| BseAI | TCCGGA | 1 cut(s) | 1233 |
| BseBI | CCWGG | 4 cut(s) | 1016, 2099, 3207, 4669 |
| BseCI | ATCGAT | 3 cut(s) | 112, 1417, 3689 |
| BseDI | CCNNGG | 6 cut(s) | 935, 1015, 1248, 3205, 4587, 4912 |
| BseGI | GGATG | 7 cut(s) | 1211, 1762, 2656, 2818, 2989, 3034, 3759 |
| BseJI | GATNNNNATC | 5 cut(s) | 1410, 1827, 2211, 3357, 3567 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 1074, 2275, 2635, 2921, 3212, 3345, 4549, 4939 |
| BseMI | GCAATG | 6 cut(s) | 372, 1911, 2657, 4010, 4080, 4356 |
| BseMII | CTCAG | 5 cut(s) | 774, 2187, 3642, 4247, 4929 |
| BseRI | GAGGAG | 2 cut(s) | 2087, 4101 |
| BseSI | GKGCMC | 6 cut(s) | 539, 1701, 3034, 3664, 4255, 4863 |
| BseXI | GCAGC | 3 cut(s) | 618, 4501, 4507 |
| BseYI | CCCAGC | 1 cut(s) | 3103 |
| Bsh1236I | CGCG | 4 cut(s) | 406, 548, 619, 639 |
| BshFI | GGCC | 9 cut(s) | 571, 1020, 1253, 1297, 1764, 2421, 2889, 3243, 4917 |
| BshNI | GGYRCC | 7 cut(s) | 1698, 1716, 2101, 3856, 4603, 4837, 4860 |
| BshVI | ATCGAT | 3 cut(s) | 112, 1417, 3689 |
| BsiHKAI | GWGCWC | 4 cut(s) | 539, 1143, 3664, 4598 |
| BsiHKCI | CYCGRG | 3 cut(s) | 2033, 2058, 4578 |
| BsiSI | CCGG | 5 cut(s) | 1234, 2648, 3473, 4589, 4601 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 1074, 2275, 2635, 2921, 3212, 3345, 4549, 4939 |
| BsmAI | GTCTC | 3 cut(s) | 2030, 2287, 2981 |
| BsmI | GAATGC | 1 cut(s) | 2785 |
| BsnI | GGCC | 9 cut(s) | 571, 1020, 1253, 1297, 1764, 2421, 2889, 3243, 4917 |
| Bso31I | GGTCTC | 2 cut(s) | 2030, 2287 |
| BsoBI | CYCGRG | 3 cut(s) | 2033, 2058, 4578 |
| Bsp1286I | GDGCHC | 9 cut(s) | 539, 1143, 1340, 1701, 3034, 3664, 4255, 4598, 4863 |
| Bsp13I | TCCGGA | 1 cut(s) | 1233 |
| Bsp1407I | TGTACA | 1 cut(s) | 1513 |
| Bsp19I | CCATGG | 1 cut(s) | 4912 |
| BspACI | CCGC | 7 cut(s) | 637, 1686, 1841, 3028, 4159, 4490, 4518 |
| BspANI | GGCC | 9 cut(s) | 571, 1020, 1253, 1297, 1764, 2421, 2889, 3243, 4917 |
| BspCNI | CTCAG | 5 cut(s) | 773, 2188, 3641, 4248, 4930 |
| BspDI | ATCGAT | 3 cut(s) | 112, 1417, 3689 |
| BspEI | TCCGGA | 1 cut(s) | 1233 |
| BspFNI | CGCG | 4 cut(s) | 406, 548, 619, 639 |
| BspMI | ACCTGC | 1 cut(s) | 441 |
| BspT107I | GGYRCC | 7 cut(s) | 1698, 1716, 2101, 3856, 4603, 4837, 4860 |
| BspTI | CTTAAG | 1 cut(s) | 4745 |
| BspTNI | GGTCTC | 2 cut(s) | 2030, 2287 |
| BsrDI | GCAATG | 6 cut(s) | 372, 1911, 2657, 4010, 4080, 4356 |
| BsrFI | RCCGGY | 1 cut(s) | 4600 |
| BsrGI | TGTACA | 1 cut(s) | 1513 |
| BssAI | RCCGGY | 1 cut(s) | 4600 |
| BssECI | CCNNGG | 6 cut(s) | 935, 1015, 1248, 3205, 4587, 4912 |
| BssNAI | GTATAC | 2 cut(s) | 757, 4674 |
| BssNI | GRCGYC | 4 cut(s) | 3857, 4007, 4604, 4838 |
| BssT1I | CCWWGG | 3 cut(s) | 935, 1248, 4912 |
| Bst1107I | GTATAC | 2 cut(s) | 757, 4674 |
| Bst2UI | CCWGG | 4 cut(s) | 1016, 2099, 3207, 4669 |
| Bst4CI | ACNGT | 6 cut(s) | 176, 292, 437, 704, 1091, 2855 |
| Bst6I | CTCTTC | 6 cut(s) | 1160, 1338, 3111, 3953, 4045, 4119 |
| BstACI | GRCGYC | 4 cut(s) | 3857, 4007, 4604, 4838 |
| BstAFI | CTTAAG | 1 cut(s) | 4745 |
| BstAUI | TGTACA | 1 cut(s) | 1513 |
| BstBAI | YACGTR | 3 cut(s) | 530, 2369, 4951 |
| BstC8I | GCNNGC | 3 cut(s) | 1839, 3030, 4594 |
| BstDSI | CCRYGG | 1 cut(s) | 4912 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 2273, 4937 |
| BstF5I | GGATG | 7 cut(s) | 1211, 1762, 2656, 2818, 2989, 3034, 3759 |
| BstFNI | CGCG | 4 cut(s) | 406, 548, 619, 639 |
| BstH2I | RGCGCY | 3 cut(s) | 3860, 4607, 4841 |
| BstMAI | GTCTC | 3 cut(s) | 2030, 2287, 2981 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 279, 554, 577, 645, 1026, 1187, 3380, 4091, 4520 |
| BstNI | CCWGG | 4 cut(s) | 1016, 2099, 3207, 4669 |
| BstNSI | RCATGY | 2 cut(s) | 3463, 4450 |
| BstSCI | CCNGG | 5 cut(s) | 1014, 2097, 3205, 4587, 4667 |
| BstSLI | GKGCMC | 6 cut(s) | 539, 1701, 3034, 3664, 4255, 4863 |
| BstSNI | TACGTA | 1 cut(s) | 530 |
| BstUI | CGCG | 4 cut(s) | 406, 548, 619, 639 |
| BstV1I | GCAGC | 3 cut(s) | 618, 4501, 4507 |
| BstV2I | GAAGAC | 4 cut(s) | 2060, 2825, 3501, 4899 |
| BstX2I | RGATCY | 5 cut(s) | 1225, 1348, 2232, 2911, 4638 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1753 |
| BstYI | RGATCY | 5 cut(s) | 1225, 1348, 2232, 2911, 4638 |
| BstZ17I | GTATAC | 2 cut(s) | 757, 4674 |
| Bsu15I | ATCGAT | 3 cut(s) | 112, 1417, 3689 |
| Bsu36I | CCTNAGG | 1 cut(s) | 4401 |
| BsuRI | GGCC | 9 cut(s) | 571, 1020, 1253, 1297, 1764, 2421, 2889, 3243, 4917 |
| BsuTUI | ATCGAT | 3 cut(s) | 112, 1417, 3689 |
| BtgI | CCRYGG | 1 cut(s) | 4912 |
| BtgZI | GCGATG | 1 cut(s) | 2825 |
| BtsCI | GGATG | 7 cut(s) | 1211, 1762, 2656, 2818, 2989, 3034, 3759 |
| BtsI | GCAGTG | 1 cut(s) | 4067 |
| BtsIMutI | CAGTG | 6 cut(s) | 1160, 1945, 3662, 4067, 4253, 4935 |
| BveI | ACCTGC | 1 cut(s) | 441 |
| Cac8I | GCNNGC | 3 cut(s) | 1839, 3030, 4594 |
| Cfr10I | RCCGGY | 1 cut(s) | 4600 |
| Cfr13I | GGNCC | 8 cut(s) | 106, 1018, 1236, 1296, 3140, 4540, 4574, 4916 |
| ClaI | ATCGAT | 3 cut(s) | 112, 1417, 3689 |
| CpoI | CGGWCCG | 1 cut(s) | 3140 |
| CseI | GACGC | 2 cut(s) | 586, 4015 |
| CsiI | ACCWGGT | 1 cut(s) | 2097 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1288, 1323 |
| CspI | CGGWCCG | 1 cut(s) | 3140 |
| DinI | GGCGCC | 3 cut(s) | 3858, 4605, 4839 |
| DraI | TTTAAA | 9 cut(s) | 118, 951, 1317, 2751, 2808, 3834, 3883, 4288, 4879 |
| DriI | GACNNNNNGTC | 1 cut(s) | 4546 |
| EaeI | YGGCCR | 3 cut(s) | 1762, 2887, 3241 |
| Eam1104I | CTCTTC | 6 cut(s) | 1160, 1338, 3111, 3953, 4045, 4119 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 4546 |
| EarI | CTCTTC | 6 cut(s) | 1160, 1338, 3111, 3953, 4045, 4119 |
| Eco105I | TACGTA | 1 cut(s) | 530 |
| Eco130I | CCWWGG | 3 cut(s) | 935, 1248, 4912 |
| Eco147I | AGGCCT | 1 cut(s) | 2421 |
| Eco24I | GRGCYC | 1 cut(s) | 1340 |
| Eco31I | GGTCTC | 2 cut(s) | 2030, 2287 |
| Eco32I | GATATC | 2 cut(s) | 139, 243 |
| Eco47I | GGWCC | 5 cut(s) | 106, 1236, 3140, 4540, 4574 |
| Eco57I | CTGAAG | 2 cut(s) | 1791, 3622 |
| Eco81I | CCTNAGG | 1 cut(s) | 4401 |
| Eco88I | CYCGRG | 3 cut(s) | 2033, 2058, 4578 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 2273, 4937 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1018, 4574 |
| EcoRI | GAATTC | 2 cut(s) | 3097, 3233 |
| EcoRII | CCWGG | 4 cut(s) | 1014, 2097, 3205, 4667 |
| EcoRV | GATATC | 2 cut(s) | 139, 243 |
| EcoT14I | CCWWGG | 3 cut(s) | 935, 1248, 4912 |
| EcoT22I | ATGCAT | 2 cut(s) | 3927, 4448 |
| EcoT38I | GRGCYC | 1 cut(s) | 1340 |
| EgeI | GGCGCC | 3 cut(s) | 3858, 4605, 4839 |
| EheI | GGCGCC | 3 cut(s) | 3858, 4605, 4839 |
| ErhI | CCWWGG | 3 cut(s) | 935, 1248, 4912 |
| FauI | CCCGC | 2 cut(s) | 3021, 4152 |
| FauNDI | CATATG | 2 cut(s) | 82, 4545 |
| FblI | GTMKAC | 3 cut(s) | 756, 4451, 4673 |
| Fnu4HI | GCNGC | 5 cut(s) | 632, 637, 4515, 4518, 4521 |
| FokI | GGATG | 7 cut(s) | 1218, 1769, 2663, 2825, 2996, 3021, 3766 |
| FriOI | GRGCYC | 1 cut(s) | 1340 |
| Fsp4HI | GCNGC | 5 cut(s) | 632, 637, 4515, 4518, 4521 |
| FspI | TGCGCA | 3 cut(s) | 444, 3897, 4892 |
| GluI | GCNGC | 5 cut(s) | 632, 637, 4515, 4518, 4521 |
| GsaI | CCCAGC | 1 cut(s) | 3107 |
| GsuI | CTGGAG | 2 cut(s) | 2294, 4512 |
| HaeII | RGCGCY | 3 cut(s) | 3860, 4607, 4841 |
| HaeIII | GGCC | 9 cut(s) | 571, 1020, 1253, 1297, 1764, 2421, 2889, 3243, 4917 |
| HapII | CCGG | 5 cut(s) | 1234, 2648, 3473, 4589, 4601 |
| HgaI | GACGC | 2 cut(s) | 586, 4015 |
| Hin1I | GRCGYC | 4 cut(s) | 3857, 4007, 4604, 4838 |
| HincII | GTYRAC | 2 cut(s) | 2471, 4707 |
| HindII | GTYRAC | 2 cut(s) | 2471, 4707 |
| HindIII | AAGCTT | 1 cut(s) | 1938 |
| HpaI | GTTAAC | 1 cut(s) | 4707 |
| HpaII | CCGG | 5 cut(s) | 1234, 2648, 3473, 4589, 4601 |
| Hpy99I | CGWCG | 6 cut(s) | 411, 467, 548, 624, 4009, 4109 |
| HpyAV | CCTTC | 7 cut(s) | 550, 1815, 2132, 3048, 3599, 4531, 4911 |
| HpyCH4III | ACNGT | 6 cut(s) | 176, 292, 437, 704, 1091, 2855 |
| HpyCH4IV | ACGT | 8 cut(s) | 409, 462, 529, 1545, 2368, 3520, 3680, 4950 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 279, 554, 577, 645, 1026, 1187, 3380, 4091, 4520 |
| HpySE526I | ACGT | 8 cut(s) | 409, 462, 529, 1545, 2368, 3520, 3680, 4950 |
| Hsp92I | GRCGYC | 4 cut(s) | 3857, 4007, 4604, 4838 |
| KasI | GGCGCC | 3 cut(s) | 3856, 4603, 4837 |
| Kpn2I | TCCGGA | 1 cut(s) | 1233 |
| KpnI | GGTACC | 1 cut(s) | 2105 |
| KspAI | GTTAAC | 1 cut(s) | 4707 |
| LmnI | GCTCC | 2 cut(s) | 1343, 4114 |
| Lsp1109I | GCAGC | 3 cut(s) | 618, 4501, 4507 |
| LweI | GCATC | 4 cut(s) | 432, 2070, 3934, 4640 |
| MabI | ACCWGGT | 1 cut(s) | 2097 |
| MaeII | ACGT | 8 cut(s) | 409, 462, 529, 1545, 2368, 3520, 3680, 4950 |
| MaeIII | GTNAC | 8 cut(s) | 248, 559, 598, 665, 1906, 2500, 2953, 4492 |
| MfeI | CAATTG | 2 cut(s) | 2977, 3620 |
| MflI | RGATCY | 5 cut(s) | 1225, 1348, 2232, 2911, 4638 |
| MhlI | GDGCHC | 9 cut(s) | 539, 1143, 1340, 1701, 3034, 3664, 4255, 4598, 4863 |
| MlsI | TGGCCA | 3 cut(s) | 1764, 2889, 3243 |
| MluNI | TGGCCA | 3 cut(s) | 1764, 2889, 3243 |
| Mly113I | GGCGCC | 3 cut(s) | 3857, 4604, 4838 |
| MlyI | GAGTC | 7 cut(s) | 1964, 2050, 2936, 3619, 3911, 4590, 4805 |
| MmeI | TCCRAC | 4 cut(s) | 1315, 2630, 3407, 4279 |
| Mox20I | TGGCCA | 3 cut(s) | 1764, 2889, 3243 |
| Mph1103I | ATGCAT | 2 cut(s) | 3927, 4448 |
| MroI | TCCGGA | 1 cut(s) | 1233 |
| MroXI | GAANNNNTTC | 2 cut(s) | 3555, 4900 |
| MscI | TGGCCA | 3 cut(s) | 1764, 2889, 3243 |
| MslI | CAYNNNNRTG | 6 cut(s) | 81, 1473, 1521, 2478, 2621, 3297 |
| Msp20I | TGGCCA | 3 cut(s) | 1764, 2889, 3243 |
| MspA1I | CMGCKG | 1 cut(s) | 4520 |
| MspCI | CTTAAG | 1 cut(s) | 4745 |
| MspI | CCGG | 5 cut(s) | 1234, 2648, 3473, 4589, 4601 |
| MspR9I | CCNGG | 5 cut(s) | 1016, 2099, 3207, 4589, 4669 |
| MteI | GCGCNGCGC | 1 cut(s) | 637 |
| MunI | CAATTG | 2 cut(s) | 2977, 3620 |
| Mva1269I | GAATGC | 1 cut(s) | 2785 |
| MvaI | CCWGG | 4 cut(s) | 1016, 2099, 3207, 4669 |
| MvnI | CGCG | 4 cut(s) | 406, 548, 619, 639 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 279, 554, 577, 645, 1026, 1187, 3380, 4091, 4520 |
| NarI | GGCGCC | 3 cut(s) | 3857, 4604, 4838 |
| NciI | CCSGG | 1 cut(s) | 4589 |
| NcoI | CCATGG | 1 cut(s) | 4912 |
| NdeI | CATATG | 2 cut(s) | 82, 4545 |
| NmeAIII | GCCGAG | 1 cut(s) | 1107 |
| NmuCI | GTSAC | 4 cut(s) | 598, 665, 2500, 4492 |
| NsbI | TGCGCA | 3 cut(s) | 444, 3897, 4892 |
| NsiI | ATGCAT | 2 cut(s) | 3927, 4448 |
| NspI | RCATGY | 2 cut(s) | 3463, 4450 |
| OliI | CACNNNNGTG | 1 cut(s) | 3297 |
| PacI | TTAATTAA | 1 cut(s) | 1365 |
| PaeR7I | CTCGAG | 1 cut(s) | 2058 |
| PaqCI | CACCTGC | 1 cut(s) | 441 |
| PceI | AGGCCT | 1 cut(s) | 2421 |
| PciI | ACATGT | 1 cut(s) | 3459 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 109, 3686 |
| PctI | GAATGC | 1 cut(s) | 2785 |
| PdmI | GAANNNNTTC | 2 cut(s) | 3555, 4900 |
| PflFI | GACNNNGTC | 2 cut(s) | 2992, 3506 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 2635, 3345 |
| PkrI | GCNGC | 5 cut(s) | 633, 638, 4516, 4519, 4522 |
| PleI | GAGTC | 7 cut(s) | 1963, 2050, 2935, 3618, 3910, 4589, 4804 |
| PluTI | GGCGCC | 3 cut(s) | 3860, 4607, 4841 |
| PpsI | GAGTC | 7 cut(s) | 1963, 2050, 2935, 3618, 3910, 4589, 4804 |
| Ppu21I | YACGTR | 3 cut(s) | 530, 2369, 4951 |
| PpuMI | RGGWCCY | 1 cut(s) | 4574 |
| PscI | ACATGT | 1 cut(s) | 3459 |
| PshBI | ATTAAT | 6 cut(s) | 1749, 1968, 2454, 2574, 3080, 3762 |
| PsiI | TTATAA | 1 cut(s) | 1325 |
| Psp1406I | AACGTT | 1 cut(s) | 3520 |
| Psp5II | RGGWCCY | 1 cut(s) | 4574 |
| Psp6I | CCWGG | 4 cut(s) | 1014, 2097, 3205, 4667 |
| PspFI | CCCAGC | 1 cut(s) | 3103 |
| PspGI | CCWGG | 4 cut(s) | 1014, 2097, 3205, 4667 |
| PspPI | GGNCC | 8 cut(s) | 106, 1018, 1236, 1296, 3140, 4540, 4574, 4916 |
| PspPPI | RGGWCCY | 1 cut(s) | 4574 |
| PsuI | RGATCY | 5 cut(s) | 1225, 1348, 2232, 2911, 4638 |
| PsyI | GACNNNGTC | 2 cut(s) | 2992, 3506 |
| RseI | CAYNNNNRTG | 6 cut(s) | 81, 1473, 1521, 2478, 2621, 3297 |
| Rsr2I | CGGWCCG | 1 cut(s) | 3140 |
| RsrII | CGGWCCG | 1 cut(s) | 3140 |
| SatI | GCNGC | 5 cut(s) | 632, 637, 4515, 4518, 4521 |
| Sau96I | GGNCC | 8 cut(s) | 106, 1018, 1236, 1296, 3140, 4540, 4574, 4916 |
| SchI | GAGTC | 7 cut(s) | 1964, 2050, 2936, 3619, 3911, 4590, 4805 |
| ScrFI | CCNGG | 5 cut(s) | 1016, 2099, 3207, 4589, 4669 |
| SduI | GDGCHC | 9 cut(s) | 539, 1143, 1340, 1701, 3034, 3664, 4255, 4598, 4863 |
| SexAI | ACCWGGT | 1 cut(s) | 2097 |
| SfaNI | GCATC | 4 cut(s) | 432, 2070, 3934, 4640 |
| SfoI | GGCGCC | 3 cut(s) | 3858, 4605, 4839 |
| Sfr274I | CTCGAG | 1 cut(s) | 2058 |
| SgrAI | CRCCGGYG | 1 cut(s) | 4600 |
| SinI | GGWCC | 5 cut(s) | 106, 1236, 3140, 4540, 4574 |
| SlaI | CTCGAG | 1 cut(s) | 2058 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 81, 1473, 1521, 2478, 2621, 3297 |
| SmlI | CTYRAG | 5 cut(s) | 1979, 2058, 2310, 4655, 4745 |
| SmoI | CTYRAG | 5 cut(s) | 1979, 2058, 2310, 4655, 4745 |
| SnaBI | TACGTA | 1 cut(s) | 530 |
| SpeI | ACTAGT | 1 cut(s) | 130 |
| SseBI | AGGCCT | 1 cut(s) | 2421 |
| SsiI | CCGC | 7 cut(s) | 637, 1686, 1841, 3028, 4159, 4490, 4518 |
| SspDI | GGCGCC | 3 cut(s) | 3856, 4603, 4837 |
| SspI | AATATT | 4 cut(s) | 123, 799, 946, 4730 |
| StuI | AGGCCT | 1 cut(s) | 2421 |
| StyD4I | CCNGG | 5 cut(s) | 1014, 2097, 3205, 4587, 4667 |
| StyI | CCWWGG | 3 cut(s) | 935, 1248, 4912 |
| TaaI | ACNGT | 6 cut(s) | 176, 292, 437, 704, 1091, 2855 |
| TaiI | ACGT | 8 cut(s) | 412, 465, 532, 1548, 2371, 3523, 3683, 4953 |
| TaqII | GACCGA | 2 cut(s) | 2331, 3157 |
| TatI | WGTACW | 6 cut(s) | 917, 1513, 1919, 2968, 3221, 4771 |
| TauI | GCSGC | 2 cut(s) | 639, 4520 |
| TscAI | CASTG | 6 cut(s) | 1160, 1952, 3662, 4074, 4260, 4942 |
| TseFI | GTSAC | 4 cut(s) | 598, 665, 2500, 4492 |
| TseI | GCWGC | 3 cut(s) | 631, 4514, 4520 |
| Tsp45I | GTSAC | 4 cut(s) | 598, 665, 2500, 4492 |
| TspGWI | ACGGA | 9 cut(s) | 30, 47, 1146, 1948, 2597, 3309, 3551, 4089, 4093 |
| TspRI | CASTG | 6 cut(s) | 1160, 1952, 3662, 4074, 4260, 4942 |
| Tth111I | GACNNNGTC | 2 cut(s) | 2992, 3506 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 2635, 3345 |
| Vha464I | CTTAAG | 1 cut(s) | 4745 |
| VneI | GTGCAC | 2 cut(s) | 535, 3660 |
| VpaK11BI | GGWCC | 5 cut(s) | 106, 1236, 3140, 4540, 4574 |
| VspI | ATTAAT | 6 cut(s) | 1749, 1968, 2454, 2574, 3080, 3762 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 2273, 4937 |
| XapI | RAATTY | 9 cut(s) | 180, 625, 1282, 1787, 3097, 3233, 3540, 3798, 3877 |
| XbaI | TCTAGA | 2 cut(s) | 1891, 3400 |
| XceI | RCATGY | 2 cut(s) | 3463, 4450 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 3206 |
| XhoI | CTCGAG | 1 cut(s) | 2058 |
| XmiI | GTMKAC | 3 cut(s) | 756, 4451, 4673 |
| XmnI | GAANNNNTTC | 2 cut(s) | 3555, 4900 |
| Zsp2I | ATGCAT | 2 cut(s) | 3927, 4448 |