RchiOBHm_Chr3g0470061

Cell division

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001165
Physical Location & Seq
Reverse (-)
16027417 .. 16032486
5070 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2424 bp
ATGACCGATAAACCTGAATCGTCCGACCCTAAGGGGACCAAGAGGGATTTCAGCACTGCGATTCTCGAGCGGAAGAAGGCGGCGAATCGCCTCGTCGTCGATGAGGCCATCAACGATGACAACTCCGTCGTCGCGCTCCACCCTGAGACCATGGAGAAGCTCCAGCTCTTCAGGGGCGACACAATCCTCATCAAGGGAAAGAAAAGGAAGGATACAATCTGCATTGCCCTTGCTGATGATACTTGTGACGAACCAAAGATTAGGATGAACAAGGTTGTGAGGAGCAATCTAAGGGTTAGGCTCGGGGATGTTGTTTCTGTTCACCAATGTGCTGATGTCAAGTACGGGAAGCGTGTGCACATTCTCCCTGTGGATGACACTATTGAAGGGGTTACTGGAAGTCTGTTTGATGCCTACTTGAAACCGTATTTCCTGGAGGCCTACCGCCCAGTGAGAAAGGGTGATCTCTTCCTCGTGAGAGGAGGAATGAGGAGTGTAGAGTTCAAGGTTATTGAGACTGATCCTTCTGAGTACTGTGTTGTTGCTCCAGACACTGAAATCTTCTGTGAGGGGGAGCCAGTAAGAAGGGAGGATGAGGATAGATTAGATGAGGTTGGTTATGATGATGTTGGTGGTGTCAGGAAGCAGATGGCTCAGATTCGTGAATTAGTGGAGCTGCCACTAAGGCATCCTCAGCTCTTTAAATCAATTGGTGTGAAGCCACCAAAGGGTATTCTGTTGTATGGGCCACCTGGATCTGGTAAAACTCTAATTGCCAGAGCTGTCGCGAATGAAACTGGGGCATTCTTTTTCTGCATCAATGGACCTGAAATCATGTCAAAATTGGCTGGGGAGAGTGAAAGCAACCTCAGGAAAGCTTTTGAGGAAGCAGAGAAGAATGCACCATCAATTATCTTTATTGATGAGATTGATTCAATTGCTCCCAAGAGAGAGAAGACACATGGAGAAGTTGAGAGGAGAATTGTGTCCCAGCTCTTGACACTTATGGATGGACTTAAATCCCGTGCACATGTAATTGTTATGGGGGCTACAAATCGTCCAAACAGTATTGACCCAGCTCTCAGAAGGTTTGGTAGATTTGATAGGGAGATAGATATTGGTGTTCCAGATGAAGTTGGGCGTCTTGAGGTCCTTCGTATTCATACAAAGAACATGAAGCTTGCTGAAGATGTTGATCTGGAAAAGATTGGCAAGGAGACACATGGTTATGTTGGTGCTGATCTTGCTGCTTTATGTACTGAGGCTGCACTCCAGTGCATCAGAGAGAAGATGGATGTCATTGACTTGGAAGATGAGGAAATAGATGCTGAGATACTCAACTCAATGGCAGTTACAAATGAACACTTCTCGACTGCTCTTGGAACAAGTAACCCATCTGCTCTTCGTGAAACGGTTGTTGAAGTGCCCAATGTTTCCTGGGATGCTATAGGAGGTCTTGAGAATGTTAAGAGAGAACTTCAAGAGACGGTTCAATATCCTGTTGAGCATCCTGAGAAATTTGAGAAATTTGGGATGTCGCCCTCAAAGGGAGTTCTATTCTATGGCCCTCCTGGGTGTGGTAAAACACTTCTTGCCAAGGCAATTGCCAATGAGTGCCAGGCAAACTTCATCAGTATCAAGGGACCTGAATTGCTTACTATGTGGTTTGGAGAGAGTGAGGCCAATGTGCGTGAAATCTTTGACAAGGCTCGTGGATCTGCTCCATGTGTCCTGTTCTTCGACGAACTTGATTCCATTGCTACTCAGAGAGGTAGCAGTTCAGGTGATGCCGGAGGTGCTGCAGATAGGGTTTTAAACCAACTCCTTACAGAGATGGACGGAATGTCCGCAAAGAAGACTGTTTTCATTATCGGCGCCACCAACAGACCTGACATTATAGACCCTGCACTTCTACGTCCGGGCCGTCTGGATCAATTGATATACATCCCTCTTCCTGATGAGGAATCCCGCCTTCAAATATTCAAGGCAGCCTTGAGGAAGTCTCCAGTTTCCAAAGAAGTGGACCTTACCGCCCTTGCCAAGTACACCCAAGGTTTCAGCGGTGCTGATATCACAGAAATTTGCCAGCGTGCTGTCAAATATGCCATCAGAGAGAATATTGAGAAGGATATTGAGAGGGAGAGGAGGAGAAGCGAGAATCCTGAGGCTATGGAGGAAGACGTGGAGGATGAAGTTCCTGAAATCAGGGCAGCTCACTTTGAGGAATCAATGAAGTTTGCTCGTCGCAGTGTTAGTGATGCGGATATCCGCAAATACCAGGCATTTGCTCAGACATTGCAGCAGTCGAGAGGATTTGGAACAGAGTTTAGGTTTCCAGAAAGTGGCTCTGCAGCAGCCGCCACTGGATCTGACCCTTTTGCAACTAGTGGTGCCGGGGCTGATGAAGATGACCTTTACAATTAG

Protein Analysis

807

Amino Acids

89.48

Weight (kDa)

5.07

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC48_N PF02359 30 - 111 1.5e-23 Cell division protein 48 (CDC48), N-terminal domain
CDC48_2 PF02933 132 - 195 1.9e-11 Cell division protein 48 (CDC48), domain 2
AAA PF00004 245 - 374 1.4e-47 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 397 - 440 2.5e-16 AAA+ lid domain
AAA PF00004 518 - 651 4.6e-47 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 673 - 712 6.8e-12 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AccB1I GGYRCC 2 cut(s) 1874, 2390
AccB7I CCANNNNNTGG 1 cut(s) 2342
AccBSI CCGCTC 1 cut(s) 70
AccII CGCG 2 cut(s) 134, 788
AclWI GGATC 5 cut(s) 515, 763, 1723, 1938, 2374
AcsI RAATTY 3 cut(s) 1517, 1526, 2079
AcuI CTGAAG 2 cut(s) 154, 1206
AcyI GRCGYC 2 cut(s) 1141, 1875
AfaI GTAC 4 cut(s) 344, 533, 1258, 2045
AfiI CCNNNNNNNGG 6 cut(s) 193, 728, 758, 1547, 1577, 2342
AflIII ACRYGT 1 cut(s) 1030
AgsI TTSAA 9 cut(s) 386, 421, 505, 936, 1421, 1481, 1493, 1976, 1984
AhdI GACNNNNNGTC 1 cut(s) 1843
AhlI ACTAGT 1 cut(s) 2384
AjiI CACGTC 1 cut(s) 2182
AjnI CCWGG 6 cut(s) 432, 751, 1436, 1570, 1617, 2277
AjuI GAANNNNNNNTTGG 2 cut(s) 32, 64
AleI CACNNNNGTG 2 cut(s) 327, 1273
Alw21I GWGCWC 2 cut(s) 360, 1030
Alw26I GTCTC 5 cut(s) 140, 509, 1211, 1478, 2007
Alw44I GTGCAC 2 cut(s) 356, 1026
AlwI GGATC 5 cut(s) 515, 763, 1723, 1938, 2374
AlwNI CAGNNNCTG 1 cut(s) 554
Ama87I CYCGRG 2 cut(s) 65, 302
AoxI GGCC 6 cut(s) 105, 438, 746, 1564, 1680, 1921
ApaLI GTGCAC 2 cut(s) 356, 1026
ApeKI GCWGC 9 cut(s) 676, 1247, 1265, 1799, 1988, 2210, 2299, 2351, 2354
ApoI RAATTY 3 cut(s) 1517, 1526, 2079
AspLEI GCGC 2 cut(s) 136, 1877
AspS9I GGNCC 8 cut(s) 36, 746, 824, 1150, 1565, 1643, 1921, 2023
AsuC2I CCSGG 2 cut(s) 1920, 2395
AsuHPI GGTGA 3 cut(s) 314, 473, 1796
AvaI CYCGRG 2 cut(s) 65, 302
AvaII GGWCC 5 cut(s) 36, 824, 1150, 1643, 2023
AxyI CCTNAGG 3 cut(s) 30, 869, 2163
BaeGI GKGCMC 3 cut(s) 360, 1030, 1428
BanI GGYRCC 2 cut(s) 1874, 2390
BauI CACGAG 2 cut(s) 473, 1710
BbsI GAAGAC 3 cut(s) 962, 1862, 2184
Bbv12I GWGCWC 2 cut(s) 360, 1030
BbvCI CCTCAGC 1 cut(s) 693
BbvI GCAGC 9 cut(s) 663, 1234, 1252, 1786, 2000, 2222, 2311, 2363, 2366
BccI CCATC 8 cut(s) 116, 643, 913, 1004, 1285, 1402, 1828, 2114
BceAI ACGGC 1 cut(s) 1908
BciT130I CCWGG 6 cut(s) 434, 753, 1438, 1572, 1619, 2279
BciVI GTATCC 1 cut(s) 205
BcnI CCSGG 2 cut(s) 1920, 2395
BcoDI GTCTC 5 cut(s) 140, 509, 1211, 1478, 2007
BcuI ACTAGT 1 cut(s) 2384
BfaI CTAG 1 cut(s) 2385
BfmI CTRYAG 3 cut(s) 1446, 1800, 2349
BfoI RGCGCY 1 cut(s) 1878
BfuI GTATCC 1 cut(s) 205
BglI GCCNNNNNGGC 1 cut(s) 685
BmcAI AGTACT 1 cut(s) 533
Bme1390I CCNGG 8 cut(s) 434, 753, 1438, 1572, 1619, 1920, 2279, 2395
Bme18I GGWCC 5 cut(s) 36, 824, 1150, 1643, 2023
BmeRI GACNNNNNGTC 1 cut(s) 1843
BmeT110I CYCGRG 2 cut(s) 65, 302
BmgBI CACGTC 1 cut(s) 2182
BmgT120I GGNCC 8 cut(s) 36, 746, 824, 1150, 1565, 1643, 1921, 2023
BmiI GGNNCC 5 cut(s) 37, 576, 1644, 1876, 2392
BmrFI CCNGG 8 cut(s) 434, 753, 1438, 1572, 1619, 1920, 2279, 2395
BmrI ACTGGG 2 cut(s) 443, 807
BmsI GCATC 9 cut(s) 400, 697, 825, 1287, 1315, 1432, 1516, 1777, 2248
BmuI ACTGGG 2 cut(s) 443, 807
BpiI GAAGAC 3 cut(s) 962, 1862, 2184
BplI GAGNNNNNCTC 2 cut(s) 1987, 2019
BpmI CTGGAG 5 cut(s) 146, 455, 531, 1256, 1989
Bpu10I CCTNAGC 1 cut(s) 693
BpuEI CTTGAG 3 cut(s) 1166, 1478, 2014
BpuMI CCSGG 2 cut(s) 1920, 2395
BsaBI GATNNNNATC 2 cut(s) 1194, 1943
BsaHI GRCGYC 2 cut(s) 1141, 1875
BsaI GGTCTC 1 cut(s) 140
BsaJI CCNNGG 6 cut(s) 150, 1437, 1571, 1596, 2050, 2394
Bsc4I CCNNNNNNNGG 6 cut(s) 193, 728, 758, 1547, 1577, 2342
Bse1I ACTGG 7 cut(s) 400, 449, 578, 802, 1273, 2006, 2368
Bse21I CCTNAGG 3 cut(s) 30, 869, 2163
Bse3DI GCAATG 3 cut(s) 222, 1755, 2294
Bse8I GATNNNNATC 2 cut(s) 1194, 1943
BseBI CCWGG 6 cut(s) 434, 753, 1438, 1572, 1619, 2279
BseDI CCNNGG 6 cut(s) 150, 1437, 1571, 1596, 2050, 2394
BseJI GATNNNNATC 2 cut(s) 1194, 1943
BseLI CCNNNNNNNGG 6 cut(s) 193, 728, 758, 1547, 1577, 2342
BseMI GCAATG 3 cut(s) 222, 1755, 2294
BseNI ACTGG 7 cut(s) 400, 449, 578, 802, 1273, 2006, 2368
BseRI GAGGAG 6 cut(s) 295, 495, 505, 991, 2158, 2161
BseSI GKGCMC 3 cut(s) 360, 1030, 1428
BseXI GCAGC 9 cut(s) 663, 1234, 1252, 1786, 2000, 2222, 2311, 2363, 2366
BseYI CCCAGC 3 cut(s) 848, 990, 1075
BsgI GTGCAG 2 cut(s) 1251, 1890
Bsh1236I CGCG 2 cut(s) 134, 788
BshFI GGCC 6 cut(s) 107, 440, 748, 1566, 1682, 1923
BshNI GGYRCC 2 cut(s) 1874, 2390
BsiHKAI GWGCWC 2 cut(s) 360, 1030
BsiHKCI CYCGRG 2 cut(s) 65, 302
BsiSI CCGG 3 cut(s) 1791, 1919, 2394
BslFI GGGAC 3 cut(s) 49, 973, 1656
BslI CCNNNNNNNGG 6 cut(s) 193, 728, 758, 1547, 1577, 2342
BsmAI GTCTC 5 cut(s) 140, 509, 1211, 1478, 2007
BsmBI CGTCTC 1 cut(s) 1478
BsmFI GGGAC 3 cut(s) 49, 973, 1656
BsmI GAATGC 2 cut(s) 803, 904
BsnI GGCC 6 cut(s) 107, 440, 748, 1566, 1682, 1923
Bso31I GGTCTC 1 cut(s) 140
BsoBI CYCGRG 2 cut(s) 65, 302
Bsp1286I GDGCHC 3 cut(s) 360, 1030, 1428
Bsp143I GATC 8 cut(s) 463, 520, 755, 1195, 1240, 1715, 1930, 2366
Bsp19I CCATGG 1 cut(s) 150
Bsp68I TCGCGA 1 cut(s) 788
BspANI GGCC 6 cut(s) 107, 440, 748, 1566, 1682, 1923
BspFNI CGCG 2 cut(s) 134, 788
BspLI GGNNCC 5 cut(s) 37, 576, 1644, 1876, 2392
BspMAI CTGCAG 2 cut(s) 1804, 2353
BspPI GGATC 5 cut(s) 515, 763, 1723, 1938, 2374
BspQI GCTCTTC 2 cut(s) 173, 1407
BspT107I GGYRCC 2 cut(s) 1874, 2390
BspTNI GGTCTC 1 cut(s) 140
BsrBI CCGCTC 1 cut(s) 70
BsrDI GCAATG 3 cut(s) 222, 1755, 2294
BsrI ACTGG 7 cut(s) 400, 449, 578, 802, 1273, 2006, 2368
BssECI CCNNGG 6 cut(s) 150, 1437, 1571, 1596, 2050, 2394
BssMI GATC 8 cut(s) 463, 520, 755, 1195, 1240, 1715, 1930, 2366
BssNI GRCGYC 2 cut(s) 1141, 1875
BssSI CACGAG 2 cut(s) 473, 1710
BssT1I CCWWGG 3 cut(s) 150, 1596, 2050
Bst2BI CACGAG 2 cut(s) 473, 1710
Bst2UI CCWGG 6 cut(s) 434, 753, 1438, 1572, 1619, 2279
Bst4CI ACNGT 6 cut(s) 426, 536, 1067, 1414, 1489, 1861
Bst6I CTCTTC 4 cut(s) 173, 473, 1407, 1956
BstACI GRCGYC 2 cut(s) 1141, 1875
BstC8I GCNNGC 3 cut(s) 1182, 2087, 2091
BstDSI CCRYGG 1 cut(s) 150
BstENI CCTNNNNNAGG 1 cut(s) 191
BstFNI CGCG 2 cut(s) 134, 788
BstH2I RGCGCY 1 cut(s) 1878
BstHHI GCGC 2 cut(s) 136, 1877
BstKTI GATC 8 cut(s) 466, 523, 758, 1198, 1243, 1718, 1933, 2369
BstMAI GTCTC 5 cut(s) 140, 509, 1211, 1478, 2007
BstMBI GATC 8 cut(s) 463, 520, 755, 1195, 1240, 1715, 1930, 2366
BstMWI GCNNNNNNNGC 5 cut(s) 685, 694, 1244, 1796, 2357
BstNI CCWGG 6 cut(s) 434, 753, 1438, 1572, 1619, 2279
BstNSI RCATGY 1 cut(s) 1034
BstSCI CCNGG 8 cut(s) 432, 751, 1436, 1570, 1617, 1918, 2277, 2393
BstSFI CTRYAG 3 cut(s) 1446, 1800, 2349
BstSLI GKGCMC 3 cut(s) 360, 1030, 1428
BstUI CGCG 2 cut(s) 134, 788
BstV1I GCAGC 9 cut(s) 663, 1234, 1252, 1786, 2000, 2222, 2311, 2363, 2366
BstV2I GAAGAC 3 cut(s) 962, 1862, 2184
BstX2I RGATCY 3 cut(s) 755, 1715, 2366
BstXI CCANNNNNNTGG 1 cut(s) 2020
BstYI RGATCY 3 cut(s) 755, 1715, 2366
Bsu36I CCTNAGG 3 cut(s) 30, 869, 2163
BsuI GTATCC 1 cut(s) 205
BsuRI GGCC 6 cut(s) 107, 440, 748, 1566, 1682, 1923
BtgI CCRYGG 1 cut(s) 150
BtrI CACGTC 1 cut(s) 2182
BtsI GCAGTG 2 cut(s) 54, 2254
BtsIMutI CAGTG 6 cut(s) 54, 456, 552, 1280, 2254, 2361
BtuMI TCGCGA 1 cut(s) 788
Cac8I GCNNGC 3 cut(s) 1182, 2087, 2091
CaiI CAGNNNCTG 1 cut(s) 554
CfoI GCGC 2 cut(s) 136, 1877
Cfr13I GGNCC 8 cut(s) 36, 746, 824, 1150, 1565, 1643, 1921, 2023
CseI GACGC 1 cut(s) 1130
Csp6I GTAC 4 cut(s) 343, 532, 1257, 2044
CspCI CAANNNNNGTGG 2 cut(s) 1693, 1728
CviAII CATG 7 cut(s) 151, 835, 962, 1031, 1174, 1223, 1725
CviQI GTAC 4 cut(s) 343, 532, 1257, 2044
DinI GGCGCC 1 cut(s) 1876
DpnI GATC 8 cut(s) 465, 522, 757, 1197, 1242, 1717, 1932, 2368
DpnII GATC 8 cut(s) 463, 520, 755, 1195, 1240, 1715, 1930, 2366
DraI TTTAAA 2 cut(s) 703, 1815
DrdI GACNNNNNNGTC 1 cut(s) 125
DriI GACNNNNNGTC 1 cut(s) 1843
DseDI GACNNNNNNGTC 1 cut(s) 125
Eam1104I CTCTTC 4 cut(s) 173, 473, 1407, 1956
Eam1105I GACNNNNNGTC 1 cut(s) 1843
EarI CTCTTC 4 cut(s) 173, 473, 1407, 1956
Eco130I CCWWGG 3 cut(s) 150, 1596, 2050
Eco147I AGGCCT 1 cut(s) 440
Eco31I GGTCTC 1 cut(s) 140
Eco32I GATATC 2 cut(s) 2071, 2266
Eco47I GGWCC 5 cut(s) 36, 824, 1150, 1643, 2023
Eco57I CTGAAG 2 cut(s) 154, 1206
Eco81I CCTNAGG 3 cut(s) 30, 869, 2163
Eco88I CYCGRG 2 cut(s) 65, 302
EcoNI CCTNNNNNAGG 1 cut(s) 191
EcoO109I RGGNCCY 2 cut(s) 1150, 1643
EcoRII CCWGG 6 cut(s) 432, 751, 1436, 1570, 1617, 2277
EcoRV GATATC 2 cut(s) 2071, 2266
EcoT14I CCWWGG 3 cut(s) 150, 1596, 2050
EgeI GGCGCC 1 cut(s) 1876
EheI GGCGCC 1 cut(s) 1876
ErhI CCWWGG 3 cut(s) 150, 1596, 2050
Esp3I CGTCTC 1 cut(s) 1478
FaeI CATG 7 cut(s) 154, 838, 965, 1034, 1177, 1226, 1728
FalI AAGNNNNNCTT 5 cut(s) 1976, 2008, 2010, 2042, 2397
FaqI GGGAC 3 cut(s) 49, 973, 1656
FatI CATG 7 cut(s) 150, 834, 961, 1030, 1173, 1222, 1724
FauI CCCGC 1 cut(s) 1976
FspBI CTAG 1 cut(s) 2385
GlaI GCGC 2 cut(s) 135, 1876
GsaI CCCAGC 3 cut(s) 852, 994, 1079
GsuI CTGGAG 5 cut(s) 146, 455, 531, 1256, 1989
HaeII RGCGCY 1 cut(s) 1878
HaeIII GGCC 6 cut(s) 107, 440, 748, 1566, 1682, 1923
HapII CCGG 3 cut(s) 1791, 1919, 2394
HgaI GACGC 1 cut(s) 1130
HhaI GCGC 2 cut(s) 136, 1877
Hin1I GRCGYC 2 cut(s) 1141, 1875
Hin1II CATG 7 cut(s) 154, 838, 965, 1034, 1177, 1226, 1728
Hin6I GCGC 2 cut(s) 134, 1875
HinP1I GCGC 2 cut(s) 134, 1875
HindIII AAGCTT 2 cut(s) 876, 1178
HinfI GANTC 9 cut(s) 17, 61, 85, 658, 932, 1751, 1964, 2158, 2225
HpaII CCGG 3 cut(s) 1791, 1919, 2394
HphI GGTGA 3 cut(s) 314, 473, 1796
Hpy166II GTNNAC 5 cut(s) 322, 358, 1028, 2023, 2046
Hpy188I TCNGA 9 cut(s) 25, 529, 657, 1085, 1283, 1767, 2111, 2292, 2371
Hpy8I GTNNAC 5 cut(s) 322, 358, 1028, 2023, 2046
Hpy99I CGWCG 6 cut(s) 98, 101, 131, 134, 1745, 2247
HpyAV CCTTC 9 cut(s) 70, 202, 380, 534, 579, 1080, 1163, 1982, 2119
HpyCH4III ACNGT 6 cut(s) 426, 536, 1067, 1414, 1489, 1861
HpyCH4IV ACGT 2 cut(s) 1915, 2181
HpyF10VI GCNNNNNNNGC 5 cut(s) 685, 694, 1244, 1796, 2357
HpySE526I ACGT 2 cut(s) 1915, 2181
Hsp92I GRCGYC 2 cut(s) 1141, 1875
Hsp92II CATG 7 cut(s) 154, 838, 965, 1034, 1177, 1226, 1728
HspAI GCGC 2 cut(s) 134, 1875
KasI GGCGCC 1 cut(s) 1874
Kzo9I GATC 8 cut(s) 463, 520, 755, 1195, 1240, 1715, 1930, 2366
LguI GCTCTTC 2 cut(s) 173, 1407
LmnI GCTCC 8 cut(s) 141, 165, 282, 550, 574, 673, 946, 1726
Lsp1109I GCAGC 9 cut(s) 663, 1234, 1252, 1786, 2000, 2222, 2311, 2363, 2366
LweI GCATC 9 cut(s) 400, 697, 825, 1287, 1315, 1432, 1516, 1777, 2248
MaeI CTAG 1 cut(s) 2385
MaeII ACGT 2 cut(s) 1915, 2181
MaeIII GTNAC 4 cut(s) 245, 391, 1351, 1388
MalI GATC 8 cut(s) 465, 522, 757, 1197, 1242, 1717, 1932, 2368
MbiI CCGCTC 1 cut(s) 70
MboI GATC 8 cut(s) 463, 520, 755, 1195, 1240, 1715, 1930, 2366
MfeI CAATTG 4 cut(s) 708, 936, 1602, 1934
MflI RGATCY 3 cut(s) 755, 1715, 2366
MhlI GDGCHC 3 cut(s) 360, 1030, 1428
Mly113I GGCGCC 1 cut(s) 1875
MmeI TCCRAC 1 cut(s) 48
MseI TTAA 4 cut(s) 702, 1017, 1467, 1814
MslI CAYNNNNRTG 3 cut(s) 327, 1227, 1273
MspA1I CMGCKG 1 cut(s) 2061
MspI CCGG 3 cut(s) 1791, 1919, 2394
MspR9I CCNGG 8 cut(s) 434, 753, 1438, 1572, 1619, 1920, 2279, 2395
MunI CAATTG 4 cut(s) 708, 936, 1602, 1934
Mva1269I GAATGC 2 cut(s) 803, 904
MvaI CCWGG 6 cut(s) 434, 753, 1438, 1572, 1619, 2279
MvnI CGCG 2 cut(s) 134, 788
MwoI GCNNNNNNNGC 5 cut(s) 685, 694, 1244, 1796, 2357
NarI GGCGCC 1 cut(s) 1875
NciI CCSGG 2 cut(s) 1920, 2395
NcoI CCATGG 1 cut(s) 150
NdeII GATC 8 cut(s) 463, 520, 755, 1195, 1240, 1715, 1930, 2366
NlaIII CATG 7 cut(s) 154, 838, 965, 1034, 1177, 1226, 1728
NlaIV GGNNCC 5 cut(s) 37, 576, 1644, 1876, 2392
NmuCI GTSAC 1 cut(s) 245
NruI TCGCGA 1 cut(s) 788
NspI RCATGY 1 cut(s) 1034
OliI CACNNNNGTG 2 cut(s) 327, 1273
PaeR7I CTCGAG 1 cut(s) 65
PceI AGGCCT 1 cut(s) 440
PciI ACATGT 1 cut(s) 1030
PciSI GCTCTTC 2 cut(s) 173, 1407
PcsI WCGNNNNNNNCGW 1 cut(s) 1921
PctI GAATGC 2 cut(s) 803, 904
PfeI GAWTC 9 cut(s) 17, 61, 85, 658, 932, 1751, 1964, 2158, 2225
PflMI CCANNNNNTGG 1 cut(s) 2342
PfoI TCCNGGA 1 cut(s) 432
PluTI GGCGCC 1 cut(s) 1878
PpuMI RGGWCCY 2 cut(s) 1150, 1643
PscI ACATGT 1 cut(s) 1030
Psp5II RGGWCCY 2 cut(s) 1150, 1643
Psp6I CCWGG 6 cut(s) 432, 751, 1436, 1570, 1617, 2277
PspFI CCCAGC 3 cut(s) 848, 990, 1075
PspGI CCWGG 6 cut(s) 432, 751, 1436, 1570, 1617, 2277
PspN4I GGNNCC 5 cut(s) 37, 576, 1644, 1876, 2392
PspPI GGNCC 8 cut(s) 36, 746, 824, 1150, 1565, 1643, 1921, 2023
PspPPI RGGWCCY 2 cut(s) 1150, 1643
PstI CTGCAG 2 cut(s) 1804, 2353
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 3 cut(s) 755, 1715, 2366
RruI TCGCGA 1 cut(s) 788
RsaI GTAC 4 cut(s) 344, 533, 1258, 2045
RsaNI GTAC 4 cut(s) 343, 532, 1257, 2044
RseI CAYNNNNRTG 3 cut(s) 327, 1227, 1273
SapI GCTCTTC 2 cut(s) 173, 1407
SaqAI TTAA 4 cut(s) 702, 1017, 1467, 1814
Sau3AI GATC 8 cut(s) 463, 520, 755, 1195, 1240, 1715, 1930, 2366
Sau96I GGNCC 8 cut(s) 36, 746, 824, 1150, 1565, 1643, 1921, 2023
ScaI AGTACT 1 cut(s) 533
ScrFI CCNGG 8 cut(s) 434, 753, 1438, 1572, 1619, 1920, 2279, 2395
SduI GDGCHC 3 cut(s) 360, 1030, 1428
SfaNI GCATC 9 cut(s) 400, 697, 825, 1287, 1315, 1432, 1516, 1777, 2248
SfcI CTRYAG 3 cut(s) 1446, 1800, 2349
SfoI GGCGCC 1 cut(s) 1876
Sfr274I CTCGAG 1 cut(s) 65
SinI GGWCC 5 cut(s) 36, 824, 1150, 1643, 2023
SlaI CTCGAG 1 cut(s) 65
SmiMI CAYNNNNRTG 3 cut(s) 327, 1227, 1273
SmlI CTYRAG 4 cut(s) 65, 1145, 1457, 1993
SmoI CTYRAG 4 cut(s) 65, 1145, 1457, 1993
SpeI ACTAGT 1 cut(s) 2384
SseBI AGGCCT 1 cut(s) 440
SspDI GGCGCC 1 cut(s) 1874
SspI AATATT 2 cut(s) 1980, 2119
SspMI CTAG 1 cut(s) 2385
StuI AGGCCT 1 cut(s) 440
StyD4I CCNGG 8 cut(s) 432, 751, 1436, 1570, 1617, 1918, 2277, 2393
StyI CCWWGG 3 cut(s) 150, 1596, 2050
TaaI ACNGT 6 cut(s) 426, 536, 1067, 1414, 1489, 1861
TaiI ACGT 2 cut(s) 1918, 2184
TaqI TCGA 5 cut(s) 66, 99, 1370, 1740, 2306
TaqII GACCGA 1 cut(s) 20
TatI WGTACW 3 cut(s) 531, 1256, 2043
TauI GCSGC 2 cut(s) 83, 2360
TfiI GAWTC 9 cut(s) 17, 61, 85, 658, 932, 1751, 1964, 2158, 2225
Tru1I TTAA 4 cut(s) 702, 1017, 1467, 1814
Tru9I TTAA 4 cut(s) 702, 1017, 1467, 1814
TscAI CASTG 6 cut(s) 61, 456, 559, 1280, 2254, 2368
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 9 cut(s) 676, 1247, 1265, 1799, 1988, 2210, 2299, 2351, 2354
Tsp45I GTSAC 1 cut(s) 245
TspGWI ACGGA 2 cut(s) 115, 1854
TspRI CASTG 6 cut(s) 61, 456, 559, 1280, 2254, 2368
Van91I CCANNNNNTGG 1 cut(s) 2342
VneI GTGCAC 2 cut(s) 356, 1026
VpaK11BI GGWCC 5 cut(s) 36, 824, 1150, 1643, 2023
XagI CCTNNNNNAGG 1 cut(s) 191
XapI RAATTY 3 cut(s) 1517, 1526, 2079
XceI RCATGY 1 cut(s) 1034
XhoI CTCGAG 1 cut(s) 65
XspI CTAG 1 cut(s) 2385
ZrmI AGTACT 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.