RchiOBHm_Chr1g0339121

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001471
Physical Location & Seq
Reverse (-)
30410604 .. 30411162
559 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
ATGCATCAACCTCAGGGTTTCACTGATCCTGTTCATCCTGATTATGTTTGCCACTTGAAGAAATCCTTATATGGATTGAAGCAAGCTCCTCGTGCTTGGAATGAAAAATTCACTTGCTTCCTTCCTACACTAGGTTTCAAATTCTCTAATTCTGATCCTAGTCTATTTGTGAAAATAACTGATCATGGTGTGATAGCTTTGCTATTATATGTGGATGATATTGTTATAACAGGCTCAGATAAACTTGGGATCACATCTATTATTTCTGAATTAAGTGATGTGTTTGACATGAAAGATCTTGGTCCATTGTCATTCTTTCTGGGAATTGGAATTAGGTATAAAGAGAAAGGACTTTTCTTGTCTCAGGAAAAGTATGCCAATGAGCTTATTCAGAAGGCTGGTCTAGAAACCTGTAGAGACTGCAATACACCATGTCTACCTCATGCTCAGTTGCTTAAAGATGAAGGCACAACATTGCTTATGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2

Protein Analysis

162

Amino Acids

18.14

Weight (kDa)

5.92

Isoelectric Point (pI)

20.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 1 - 146 3.3e-37 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 227
AccI GTMKAC 1 cut(s) 436
AclWI GGATC 3 cut(s) 20, 149, 257
AcsI RAATTY 2 cut(s) 107, 140
AfiI CCNNNNNNNGG 1 cut(s) 131
AgsI TTSAA 3 cut(s) 58, 79, 139
AluBI AGCT 3 cut(s) 86, 197, 385
AluI AGCT 3 cut(s) 86, 197, 385
Alw26I GTCTC 2 cut(s) 366, 411
AlwI GGATC 3 cut(s) 20, 149, 257
ApoI RAATTY 2 cut(s) 107, 140
AspS9I GGNCC 1 cut(s) 302
AvaII GGWCC 1 cut(s) 302
AxyI CCTNAGG 1 cut(s) 12
BauI CACGAG 1 cut(s) 90
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BclI TGATCA 1 cut(s) 181
BcoDI GTCTC 2 cut(s) 366, 411
BfaI CTAG 3 cut(s) 131, 159, 404
BfmI CTRYAG 1 cut(s) 412
BglII AGATCT 1 cut(s) 295
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 302
BmsI GCATC 1 cut(s) 13
Bsc4I CCNNNNNNNGG 1 cut(s) 131
Bse21I CCTNAGG 1 cut(s) 12
Bse3DI GCAATG 1 cut(s) 473
BseGI GGATG 2 cut(s) 34, 220
BseLI CCNNNNNNNGG 1 cut(s) 131
BseMI GCAATG 1 cut(s) 473
BseMII CTCAG 4 cut(s) 26, 249, 377, 461
BseRI GAGGAG 1 cut(s) 78
BslI CCNNNNNNNGG 1 cut(s) 131
BsmAI GTCTC 2 cut(s) 366, 411
Bsp143I GATC 5 cut(s) 25, 154, 181, 249, 295
BspCNI CTCAG 4 cut(s) 25, 248, 376, 460
BspPI GGATC 3 cut(s) 20, 149, 257
BsrDI GCAATG 1 cut(s) 473
BssMI GATC 5 cut(s) 25, 154, 181, 249, 295
BssSI CACGAG 1 cut(s) 90
Bst2BI CACGAG 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 4 cut(s) 12, 235, 363, 447
BstENI CCTNNNNNAGG 1 cut(s) 129
BstF5I GGATG 2 cut(s) 34, 220
BstKTI GATC 5 cut(s) 28, 157, 184, 252, 298
BstMAI GTCTC 2 cut(s) 366, 411
BstMBI GATC 5 cut(s) 25, 154, 181, 249, 295
BstMWI GCNNNNNNNGC 1 cut(s) 92
BstSFI CTRYAG 1 cut(s) 412
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
Bsu36I CCTNAGG 1 cut(s) 12
BtsCI GGATG 2 cut(s) 34, 220
BtsIMutI CAGTG 1 cut(s) 21
Cac8I GCNNGC 1 cut(s) 84
Cfr13I GGNCC 1 cut(s) 302
CviAII CATG 4 cut(s) 185, 289, 432, 443
CviJI RGCY 5 cut(s) 86, 197, 234, 385, 398
CviKI_1 RGCY 5 cut(s) 86, 197, 234, 385, 398
DdeI CTNAG 4 cut(s) 12, 235, 363, 447
DpnI GATC 5 cut(s) 27, 156, 183, 251, 297
DpnII GATC 5 cut(s) 25, 154, 181, 249, 295
Eco47I GGWCC 1 cut(s) 302
Eco81I CCTNAGG 1 cut(s) 12
EcoNI CCTNNNNNAGG 1 cut(s) 129
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 4 cut(s) 188, 292, 435, 446
FalI AAGNNNNNCTT 2 cut(s) 50, 82
FatI CATG 4 cut(s) 184, 288, 431, 442
FbaI TGATCA 1 cut(s) 181
FblI GTMKAC 1 cut(s) 436
FokI GGATG 2 cut(s) 21, 227
FspBI CTAG 3 cut(s) 131, 159, 404
Hin1II CATG 4 cut(s) 188, 292, 435, 446
Hpy166II GTNNAC 1 cut(s) 437
Hpy188I TCNGA 4 cut(s) 154, 238, 268, 393
Hpy188III TCNNGA 3 cut(s) 38, 365, 404
Hpy8I GTNNAC 1 cut(s) 437
HpyAV CCTTC 3 cut(s) 131, 388, 458
HpyCH4V TGCA 2 cut(s) 4, 423
HpyF10VI GCNNNNNNNGC 1 cut(s) 92
HpyF3I CTNAG 4 cut(s) 12, 235, 363, 447
Hsp92II CATG 4 cut(s) 188, 292, 435, 446
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 5 cut(s) 25, 154, 181, 249, 295
LmnI GCTCC 1 cut(s) 91
LpnPI CCDG 7 cut(s) 42, 51, 216, 305, 350, 384, 424
LweI GCATC 1 cut(s) 13
MaeI CTAG 3 cut(s) 131, 159, 404
MalI GATC 5 cut(s) 27, 156, 183, 251, 297
MboI GATC 5 cut(s) 25, 154, 181, 249, 295
MboII GAAGA 1 cut(s) 70
MflI RGATCY 1 cut(s) 295
MluCI AATT 6 cut(s) 107, 140, 148, 269, 324, 330
MnlI CCTC 3 cut(s) 21, 99, 450
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 272, 456
MwoI GCNNNNNNNGC 1 cut(s) 92
NdeII GATC 5 cut(s) 25, 154, 181, 249, 295
NlaIII CATG 4 cut(s) 188, 292, 435, 446
NsiI ATGCAT 1 cut(s) 6
PsiI TTATAA 1 cut(s) 227
PspPI GGNCC 1 cut(s) 302
PsuI RGATCY 1 cut(s) 295
SaqAI TTAA 2 cut(s) 272, 456
Sau3AI GATC 5 cut(s) 25, 154, 181, 249, 295
Sau96I GGNCC 1 cut(s) 302
SetI ASST 8 cut(s) 13, 88, 136, 199, 338, 387, 413, 442
SfaNI GCATC 1 cut(s) 13
SfcI CTRYAG 1 cut(s) 412
SinI GGWCC 1 cut(s) 302
Sse9I AATT 6 cut(s) 107, 140, 148, 269, 324, 330
SspMI CTAG 3 cut(s) 131, 159, 404
TasI AATT 6 cut(s) 107, 140, 148, 269, 324, 330
Tru1I TTAA 2 cut(s) 272, 456
Tru9I TTAA 2 cut(s) 272, 456
TscAI CASTG 1 cut(s) 28
TspDTI ATGAA 4 cut(s) 23, 117, 305, 477
TspRI CASTG 1 cut(s) 28
VpaK11BI GGWCC 1 cut(s) 302
XagI CCTNNNNNAGG 1 cut(s) 129
XapI RAATTY 2 cut(s) 107, 140
XbaI TCTAGA 1 cut(s) 403
XmiI GTMKAC 1 cut(s) 436
XspI CTAG 3 cut(s) 131, 159, 404
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.